CN102925385A - Compound microorganism bacterium agent for treating poultry excrement to prepare composts and preparation method of compound microorganism bacterium agent - Google Patents
Compound microorganism bacterium agent for treating poultry excrement to prepare composts and preparation method of compound microorganism bacterium agent Download PDFInfo
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- CN102925385A CN102925385A CN2012104028187A CN201210402818A CN102925385A CN 102925385 A CN102925385 A CN 102925385A CN 2012104028187 A CN2012104028187 A CN 2012104028187A CN 201210402818 A CN201210402818 A CN 201210402818A CN 102925385 A CN102925385 A CN 102925385A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
The invention discloses a compound microorganism bacterium agent for treating poultry excrement to prepare composts and a preparation method of the compound microorganism bacterium agent. The compound microorganism bacterium agent is prepared from bacillus megaterium, Saccharomyces cerevisiae, bacillus licheniformis, streptomyces albogriseolus and lactobacillus plantarum. The preparation method comprises the following steps of: A. cultivation of the bacillus megaterium and the bacillus licheniformis, to be specific, inoculating the bacillus megaterium and the bacillus licheniformis to a beef extract peptone solid medium for cultivation; B. cultivation of the Saccharomyces cerevisiae, to be specific, inoculating the Saccharomyces cerevisiae to a lactic acid-potato-glucose solid medium for cultivation; C. cultivation of the lactobacillus plantarum, to be specific, inoculating the lactobacillus plantarum on an MRS (De Man, Rogosa and Sharpe) medium to a solid medium for cultivation; D. cultivation of the streptomyces albogriseolus, to be specific, inoculating the streptomyces albogriseolus to a solid medium for cultivation; and E. stirring to mix the cultivated bacterium agent, and ventilating and drying the bacterium agent at a low temperature to obtain the compound microorganism preparation. The compound microorganism bacterium agent for treating the poultry excrement to prepare the composts and the preparation method of the compound microorganism bacterium agent disclosed by the invention have the advantages of reasonable formula, capability of quickly removing smell of the poultry excrement during the treatment and producing organic fertilizers through decomposed conversion, easy implementation as well as simplicity and convenience for operation.
Description
Technical field
The invention belongs to microbial technology field, more specifically relate to a kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, the preparation method who also relates to simultaneously this biotechnological formulation, said preparation can be widely used in the processing of large-scale plant feces of livestock and poultry, the speed of becoming thoroughly decomposed is fast, easy to implement the method, easy and simple to handle.
Background technology
In recent years, livestock and poultry cultivation is already from traditional courtyard-style cultivation to intensive, mass-producing, commercialization future development.The existing pig in the whole nation, ox, chicken three major types livestock and poultry large-scale cultivation field be 3,900,000 places approximately, and wherein, all kinds of livestock and poultry large-scale cultivations residential quarter reaches more than 40,000, produces approximately 3,000,000,000 tons of feces of livestock and poultry total amounts per year.The Hubei Province live pig was delivered 3,790 ten thousand for sale in 2007,2,705 ten thousand of livestock on hands; Poultry is delivered 43,340 ten thousand for sale, 30,406 ten thousand of livestock on hands; Ox is delivered 1,400,000 for sale, 3,860,000 of livestock on hands.These livestock and poultry farms are when building, and most not enough to the dirty processing and utilizing facility consideration of its excrement, 70% ight soil is without effective processing, and just arbitrarily discharging has caused the problem of environmental pollution that is on the rise.At present, the prevention and cure of pollution of livestock and poultry cultivation are processed and are included in the whole nation " the moderately well-off environmental action plan in rural area " of Eleventh Five-Year Plan enforcement as Important Project.Feces of livestock and poultry is waste, also is the huge manure resources of agriculture production, and allowing it run off in a large number or giving it up is the huge waste of resource.Utilizing feces of livestock and poultry to produce biological organic fertilizer is that minimizing is polluted, one of best way turns waste into wealth.
Enter the eighties in last century, begun in the world to be used for the research of the organism of fermentation of organic fertilizer production, purpose is in order to shorten fermentation period, to reach the organic materials quick composting, take Japanese EM, BYM(enzymatic microorganism) begin to use in organic fertilizer production as a batch fermentation Inoculant of representative.
The exploitation of Chinese biological Industrialization of Organic Fertilizer is started late, and the introduction of EM, BYM has expedited the emergence of the rise of Chinese biological Organic fertilizer industry.The Chinese biological fertilizer has been established the thinking of development that is converted into purpose take the recycling of organic waste as basis, industrialization production as approach, product ommercialization, base oneself upon distinct Chinese characteristics, research and development and the industrialization of technology, technique, equipment, microorganism advance synchronously, all obtain certain achievement at aspects such as biological organic fertilizer zymotechnique level, equipment and industrialization productions.
New development mainly is During High-Temperature Composting microbial inoculant and bacterial manure development and application aspect microbial technique.The one, Inoculant improves the research of raw material degradation speed.The 2nd, the research that Inoculant improves fertilizer efficiency and produces bacterial manure.The 3rd, Inoculant improves the research of compost net effect.
At present, the development of Chinese biological fertilizer industry also begins to take shape, yet because the microorganism kind is relatively single, the control of the parameter of production process is incomplete, industry engineer equipment level is lower, become the key of restriction Industry Quick Development, the biological organic fertilizer technology trends will be from addressing the above problem the development of starting with to promote industry.1. the research and development of strengthening function property complex micro organism fungicide, the development of microbiobacterial agent will compound towards bacterial strain, numerous in variety future development.2. setting up the process control parameters model that is fit to enterprise's production and quality control, is the key that guarantees enterprise product characteristic, stabilized product quality.
Summary of the invention
The objective of the invention is to be to provide a kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, prescription rationally, easy to use, this microbial inoculum is mainly used in the feces of livestock and poultry of vast plant and processes, and is translated into the biological organic fertilizer of efficient, safety, environmental protection.Energy quick composting feces of livestock and poultry can will become thoroughly decomposed fully at 7-10 days, solve feces of livestock and poultry and process problem difficult, environmental pollution, be converted into organic fertilizer, improve soil organic matter content, played the effect of " land used is increased soil fertility ", had significant economic benefit, social benefit and environmental benefit.
Another object of the present invention is to be to provide a kind of preparation method who makes the complex microorganism preparations of compost for the treatment of feces of livestock and poultry, and easy to implement the method, easy and simple to handle, production cost is low.
In order to realize above-mentioned purpose, the present invention adopts following technical measures:
Technical conceive of the present invention is: this project adopts modern microbial fermentation engineering technology, the multiple function stem that can efficiently produce proteolytic enzyme, cellulase, hemicellulase and lignoenzyme of screening and culturing is made up cultivation and becomes a kind of complex micro organism fungicide, mainly consisted of by 5 kinds of multi-functional bacterial strains such as bacillus megaterium, Bacillus licheniformis, yeast saccharomyces cerevisiae, plant lactobacillus, white light streptomycetes, have that strain combinations is new, structure is steady, live strong, bacterium of enzyme counts high.
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it is formed by the preparation of raw material of following weight per-cent:
Described bacillus megaterium derives from Chinese Typical Representative culture collection center (Wuhan University preservation center is purchased)
Described yeast saccharomyces cerevisiae derives from Chinese Typical Representative culture collection center (Wuhan University preservation center is purchased)
Described Bacillus licheniformis derives from Chinese Typical Representative culture collection center (Wuhan University preservation center is purchased)
Described white light Streptomyces is (Wuhan University preservation center is purchased) in Chinese Typical Representative culture collection center
Described plant lactobacillus derives from Chinese Typical Representative culture collection center (Wuhan University preservation center is purchased)
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it forms (preferable range) by the preparation of raw material of following weight per-cent:
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it forms (optimum range) by the preparation of raw material of following weight per-cent:
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it forms (best proportioning) by the preparation of raw material of following weight per-cent:
A kind of complex microorganism preparations method of making compost for the treatment of feces of livestock and poultry the steps include:
The cultivation of A, bacillus megaterium, Bacillus licheniformis: bacillus megaterium, Bacillus licheniformis are seeded in respectively on the beef-protein medium and (see " Microbiology Experiment ", extractum carnis 3g, peptone 10g, sodium-chlor 5g, agar 20g, distilled water 1000mL, pH 7.0～8.0), 28-30 ° of C, making primary inclined plane cultivates, then be inoculated into and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the beef extract-peptone solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium.
The cultivation of B, yeast saccharomyces cerevisiae: yeast saccharomyces cerevisiae (is seen " Microbiology Experiment " on lactic acid-potato-dextrose culture-medium, lactic acid (5ml), potato (200 gram), glucose (20 gram), agar (15-20 gram), distilled water (1000ml)), 28-30 ° of C, making primary inclined plane cultivates, then be inoculated into and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
The cultivation of C, plant lactobacillus: on the MRS substratum, (see " Microbiology Experiment ", the lactic-acid-bacterium substratum), 28-30 ° of C, making primary inclined plane cultivates, then be inoculated into and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-5.0 * 10
7Individual/the gram solid medium;
The cultivation of D, white light streptomycete: white light streptomycete at first (sees " Microbiology Experiment ") on the Gause I substratum, 28-30 ° of C, making primary inclined plane cultivates, then be inoculated into and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium.
E, cultured microbial inoculum in above-mentioned A, B, C, the D step is mixed by certain mass ratio, low temperature (10-60 ° of C) air seasoning, moisture content is controlled at below 30%, just obtains complex microorganism preparations.
The present invention fills a prescription rationally, and the processing feces of livestock and poultry is eliminated stink fast, becoming thoroughly decomposed is converted into organic fertilizer, easy to implement the method, easy and simple to handle.
This microbial inoculum is mainly used in the feces of livestock and poultry of vast plant and processes, and is translated into the biological organic fertilizer of efficient, safety, environmental protection.The product energy quick composting feces of livestock and poultry of our company's research and development, can will become thoroughly decomposed fully at 7-10 days, solve feces of livestock and poultry and processed problem difficult, environmental pollution, be converted into organic fertilizer, improve soil organic matter content, play the effect of " land used is increased soil fertility ", have significant economic benefit, social benefit and environmental benefit.
The present invention compared with prior art has the following advantages and effect:
1. all can start fast under low temperature (0 ℃) or high temperature, the use of can becoming thoroughly decomposed throughout the year is not subject to seasonal restrictions.
2. the compound proportioning science of bacterial classification, and can be according to the nutrient allotment bacterial classification kind of different material, pointed strong, the characteristics such as the time of becoming thoroughly decomposed is short.
3. can suitably add the use of " primer-detonators " such as glucose, sucrose, molasses, protein.
4. accelerate the formation of the organic materials decomposition conversion rate such as feces of livestock and poultry and soil ulmin.
5. discharge available nutrient in the digest process, produce the material of the multiple Promoting plant growths such as a large amount of amino acid, organic acid, VITAMIN, polysaccharide, enzyme, plant hormone.
6. after the fertilizer that becomes thoroughly decomposed is manured into soil, improve fast Soil structure, improve the soil water-reataining fertilizer-keeping ability; Enrich soil microflora, increase soil fertility.Can promote crop root to grow, robust growth strengthens drought resisting, the resistance such as disease-resistant, reduces disease to occur, and improves quality of agricultural product.
7. effectively kill disease worm's ovum, weed seed, dewater, deodorization.
Embodiment
Embodiment 1:
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it is formed by the preparation of raw material of following weight per-cent:
A kind of complex microorganism preparations method of making compost for the treatment of feces of livestock and poultry the steps include:
The cultivation of A, bacillus megaterium, Bacillus licheniformis: bacillus megaterium, Bacillus licheniformis are seeded in respectively on the beef-protein medium, 28 or 29 or 30 ° of C, making primary inclined plane cultivates, then be inoculated into and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium.
The cultivation of B, yeast saccharomyces cerevisiae: yeast saccharomyces cerevisiae is on lactic acid-potato-dextrose culture-medium, 28 or 29 or 30 ° of C, make primary inclined plane and cultivate, then be inoculated into and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium;
The cultivation of C, plant lactobacillus: on the MRS substratum, 28 or 29 or 30 ° of C make primary inclined plane and cultivate, and then are inoculated into and do vibration secondary liquid culture in the triangular flask, are inoculated at last to do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-5.0 * 10
7Individual/the gram solid medium;
The cultivation of D, white light streptomycete: white light streptomycete is at first on the Gause I substratum, 28 or 29 or 30 ° of C, make primary inclined plane and cultivate, then be inoculated into and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 10
8Individual/the gram solid medium.
E, cultured microbial inoculum in above-mentioned A, B, C, the D step is mixed by certain mass ratio, low temperature (10 or 15 or 25 or 35 or 46 or 54 or 60 ° of C) air seasoning, moisture content is controlled at below 30%, just obtains complex microorganism preparations.
The present invention fills a prescription rationally, and the processing feces of livestock and poultry is eliminated stink fast, becoming thoroughly decomposed is converted into organic fertilizer, easy to implement the method, easy and simple to handle.
Embodiment 2:
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it is formed by the preparation of raw material of following weight per-cent:
Its preparation process is identical with embodiment 1.
Embodiment 3:
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it is formed by the preparation of raw material of following weight per-cent:
Its preparation process is identical with embodiment 1.
Embodiment 4:
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it is formed by the preparation of raw material of following weight per-cent:
Its preparation process is identical with embodiment 1.
Embodiment 5:
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it is formed by the preparation of raw material of following weight per-cent:
Its preparation process is identical with embodiment 1.
Embodiment 6:
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it is formed by the preparation of raw material of following weight per-cent:
Its preparation process is identical with embodiment 1.
Embodiment 7:
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it is formed by the preparation of raw material of following weight per-cent:
Its preparation process is identical with embodiment 1.
Embodiment 8:
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it is formed by the preparation of raw material of following weight per-cent:
Its preparation process is identical with embodiment 1.
Embodiment 9:
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it is formed by the preparation of raw material of following weight per-cent:
Its preparation process is identical with embodiment 1.
Embodiment 10:
A kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it is formed by the preparation of raw material of following weight per-cent:
Its preparation process is identical with embodiment 1.
The explanation of product operation technique:
1, raw material is prepared: for the permeability that improves material, reduce cost etc., can increase an amount of crop material (corn stalk of 20-50%, wheat straw, rice straw etc.), it is brokenly mixed with ight soil.For subsequent use.
2, actication of culture (being mixed once): get 5 kilograms of compost fermentation agent and 95 kilograms of materials for subsequent use mix, add uniformly water simultaneously, water content is controlled at 50-65%, natural packing 24-48 hour.Stand-by.
3, composting and fermentation management
100 kilograms of materials after the activation are directly mixed with 900 kilograms of raw materials for subsequent use, and water content is controlled at 50-65%; Specification wide according to 1.5-2 rice, that 1.0-1.5 rice is high heap processed; Heap pond fermentation heap height is 1.0-1.2 rice, and sets up ventilating pit.Every day, the Timing measurement material temperature began the overturning cooling oxygen supply, once a day when material temperature is raised to 50 ℃, the heap temperature surpasses 65 ℃, increases the overturning number of times, keeps high temperature and becomes thoroughly decomposed approximately 3-7 days stage, until fully become thoroughly decomposed, sign: heap temperature drop low, material loosening, powdery, chocolate, be as good as stink.
Precaution:
1. deposit shady and cool dry place, forbid that high light is exposed to the sun,
2. avoid using with strong acid and strong base easy volatile chemical and mixed the putting of sterilant
3. according to different material, envrionment temperature, the conditions such as moisture content can be adjusted consumption as one sees fit.
Claims (10)
1. complex microorganism preparations of making compost for the treatment of feces of livestock and poultry, it is formed by the preparation of raw material of following weight per-cent:
Raw material weight per-cent
Bacillus megaterium 5%-50%
Yeast saccharomyces cerevisiae 2%-35%
Bacillus licheniformis 5%-30%
Bai Qianse streptomycete 2%-30%
Plant lactobacillus 2%-20%.
2. a kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry according to claim 1 is characterized in that:
Raw material weight per-cent
Bacillus megaterium 12%-45%
Yeast saccharomyces cerevisiae 8%-30%
Bacillus licheniformis 10%-25%
Bai Qianse streptomycete 5%-25%
Plant lactobacillus 5%-20%.
3. a kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry according to claim 1 is characterized in that:
Raw material weight per-cent
Bacillus megaterium 18%-35%
Yeast saccharomyces cerevisiae 15%-30%
Bacillus licheniformis 15%-25%
Bai Qianse streptomycete 10%-20%
Plant lactobacillus 10%-15%.
4. a kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry according to claim 1 is characterized in that:
Raw material weight per-cent
Bacillus megaterium 20%
Yeast saccharomyces cerevisiae 20%
Bacillus licheniformis 25%
Bai Qianse streptomycete 20%
Plant lactobacillus 15%.
5. a kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry according to claim 1 is characterized in that:
Raw material weight per-cent
Bacillus megaterium 25%
Yeast saccharomyces cerevisiae 25%
Bacillus licheniformis 20%
Bai Qianse streptomycete 15%
Plant lactobacillus 15%.
6. a kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry according to claim 1 is characterized in that:
Raw material weight per-cent
Bacillus megaterium 35%
Yeast saccharomyces cerevisiae 25%
Bacillus licheniformis 15%
Bai Qianse streptomycete 15%
Plant lactobacillus 10%.
7. a kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry according to claim 1 is characterized in that:
Raw material weight per-cent
Bacillus megaterium 30%
Yeast saccharomyces cerevisiae 25%
Bacillus licheniformis 20%
Bai Qianse streptomycete 15%
Plant lactobacillus 10%.
8. a kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry according to claim 1 is characterized in that:
Raw material weight per-cent
Bacillus megaterium 33%
Yeast saccharomyces cerevisiae 27%
Bacillus licheniformis 18%
Bai Qianse streptomycete 16%
Plant lactobacillus 6%.
9. a kind of complex microorganism preparations of making compost for the treatment of feces of livestock and poultry according to claim 1 is characterized in that:
Raw material weight per-cent
Bacillus megaterium 25%
Yeast saccharomyces cerevisiae 15%
Bacillus licheniformis 25%
Bai Qianse streptomycete 20%
Plant lactobacillus 15%.
10. a kind of complex microorganism preparations method of making compost for the treatment of feces of livestock and poultry claimed in claim 1 the steps include:
The cultivation of A, bacillus megaterium, Bacillus licheniformis: bacillus megaterium, Bacillus licheniformis are seeded in respectively on the beef-protein medium: extractum carnis 3g, peptone 10g, sodium-chlor 5g, agar 20g, distilled water 1000mL, pH 7.0～8.0,28-300C, making primary inclined plane cultivates, then be inoculated into and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the beef extract-peptone solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 108/gram solid medium;
The cultivation of B, yeast saccharomyces cerevisiae: yeast saccharomyces cerevisiae is on lactic acid-potato-dextrose culture-medium: lactic acid 5ml, potato 200 grams, glucose 20 grams, agar 15-20 gram, distilled water 1000ml, 28-300C, making primary inclined plane cultivates, then be inoculated into and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 108/gram solid medium;
The cultivation of C, plant lactobacillus: on the MRS substratum, 28-300C, make primary inclined plane and cultivate, then be inoculated into and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-5.0 * 107/gram solid medium;
The cultivation of D, white light streptomycete: white light streptomycete is at first on the Gause I substratum, 28-300C, making primary inclined plane cultivates, then be inoculated into and do vibration secondary liquid culture in the triangular flask, be inoculated at last and do three grades on the solid medium and be cultured to the bacterium number and reach 1.0-2.5 * 108/gram solid medium;
E, cultured microbial inoculum in above-mentioned A, B, C, the D step is mixed in mass ratio, low-temperature aeration is dry, and moisture content is controlled at below 30%, obtains complex microorganism preparations.
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