CN110157644A - A kind of purposes of microbial deoderizer - Google Patents
A kind of purposes of microbial deoderizer Download PDFInfo
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- CN110157644A CN110157644A CN201910456325.3A CN201910456325A CN110157644A CN 110157644 A CN110157644 A CN 110157644A CN 201910456325 A CN201910456325 A CN 201910456325A CN 110157644 A CN110157644 A CN 110157644A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/20—Nature of the water, waste water, sewage or sludge to be treated from animal husbandry
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/02—Odour removal or prevention of malodour
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
- C12R2001/11—Bacillus megaterium
Abstract
The invention discloses a kind of bacillus megaterium Z3 (Bacillus megaterium Z3) for being CCTCC NO:M 2018660 including the Bacillus flexus Z2 (Bacillus flexus Z2) that deposit number is CCTCC NO:M 2018659 or/and its culture or/and its machining object or/and deposit number or/and its culture or/and its machining objects.Above-mentioned two bacterial strain has oxidation, and high temperature resistant to ammonia and hydrogen sulfide.Bacillus flexus dry powder or bacillus megaterium dry powder made of above-mentioned bacterial strains or Bacillus flexus culture solution or bacillus megaterium culture solution impose in feces of livestock and poultry or landfill leachate or Bacillus flexus is mixed according to the ratio uniform of 0~100:0~100 with bacillus megaterium and imposed in the object to be processed for needing biological deodorizing.
Description
Technical field
The present invention relates to microorganisms technical fields, more particularly, to a kind of purposes of microbial deoderizer.
Background technique
Feces of livestock and poultry can generate a large amount of pernicious gases in composting process, and wherein ammonia and hydrogen sulfide are most important stenches
Substance jeopardizes the health of surrounding resident, also results in livestock and poultry cultivation ring if dealing with improperly can cause seriously to pollute to ambient atmosphere
Border deteriorates, and directly affects the sound development of aquaculture.The release of ammonia and hydrogen sulfide can also reduce nitrogen and sulphur in compost product
The content of element, influences quality of compost products.Therefore it should adopt an effective measure and reduce the release of ammonia and hydrogen sulfide in composting process,
Convert it into available substance.
Many deodorization bacterium of domestic screening are tested at normal temperature at present shows preferable deodorizing effect, and feces of livestock and poultry
When compost, hot stage is the critical stage of high temperature aerobic compostingization processing organic solid castoff, and most of organic matter is herein
It is oxidized decomposition, ammonia and hydrogen sulfide burst size highest in the process, however the high temperature resistance of current deodorization bacterium is poor, organic
Application is not high in compost field.
Summary of the invention
One of the objects of the present invention is to provide a kind of pair of ammonias and hydrogen sulfide to have oxidation, and resistant to high temperature
Microbial deoderizer.
The second object of the present invention is mentioned microorganism deodorizing microorganism being used for biological deodorizing.
To achieve the above object, the technical scheme adopted by the invention is as follows: a kind of microbial deoderizer contains deposit number
For the Bacillus flexus Z2 (Bacillus flexus Z2) of CCTCC NO:M 2018659 or/and its culture or/and its
Machining object.The deposit number of Bacillus flexus Z2 (Bacillus flexus Z2) are as follows: CCTCC NO:M 2018659；Classification
Name are as follows: Bacillus flexus Z2 (Bacillus flexus Z2)；Preservation date are as follows: on September 30th, 2018；Depositary institution
Are as follows: China typical culture collection center (CCTCC)；Depositary institution address are as follows: the Chinese Wuhan Wuhan University.
A kind of microbial deoderizer further includes the bacillus megaterium Z3 that deposit number is CCTCC NO:M 2018660
(Bacillus megaterium Z3) or/and its culture or/and its machining object.Bacillus megaterium Z3 (Bacillus
Megaterium Z3) deposit number are as follows: CCTCC NO:M 2018660；Classification naming are as follows: bacillus megaterium Z3
(Bacillus megaterium Z3)；Preservation date are as follows: on September 30th, 2018；Depositary institution are as follows: Chinese Typical Representative culture is protected
Hiding center (CCTCC)；Depositary institution address are as follows: the Chinese Wuhan Wuhan University.
Bacillus flexus Z2 (Bacillus flexus Z2) resistance to 85 DEG C high temperature.
Bacillus megaterium Z3 (Bacillus megaterium Z3) resistance to 85 DEG C high temperature.
A kind of microbial deoderizer, the Bacillus flexus Z2 for being CCTCC NO:M 2018659 containing deposit number
(Bacillus flexus Z2) or/and its culture or/and its machining object, and containing deposit number is CCTCC NO:M
2018660 bacillus megaterium Z3 (Bacillus megaterium Z3) or/and its culture or/and its machining object.
Bacillus flexus Z2 (Bacillus flexus Z2) or/and bacillus megaterium Z3 (Bacillus
Megaterium Z3) it is used for biological deodorizing.
Bacillus flexus Z2 (Bacillus flexus Z2) dry powder or bacillus megaterium Z3 (Bacillus
Megaterium Z3) dry powder or Bacillus flexus Z2 (Bacillus flexus Z2) culture solution or bacillus megaterium Z3
(Bacillus megaterium Z3) culture solution or Bacillus flexus Z2 (Bacillus flexus Z2) and huge gemma
The mixed liquor that bacillus Z3 (Bacillus megaterium Z3) is mixed to get according to the ratio uniform of 0~100:0~100, is applied
In the object to be processed for needing biological deodorizing.
Compared with prior art, the invention has the benefit that
1, the feces of livestock and poultry in animal house handle not in time can be decomposed by the microorganisms generate pernicious gas (mainly include ammonia and
Hydrogen sulfide).When ammonia level is more than 26mg/m in animal house air3, livestock and poultry can be made to generate disease, the serious growth for reducing livestock and poultry
The utilization rate of speed and feed；The content of Air Hydrogen Sulfide is more than 10mg/m in giving up3, livestock and poultry can be made to be poisoned or cause tissue
Anoxic can also make livestock and poultry generate the illnesss such as slow poisoning even the hydrogen sulfide of low concentration, under long term.The present invention is bent bud
Spore bacillus and bacillus megaterium can the efficient oxidation ammonia and hydrogen sulfide gas, provide excellent environment for growth of animals or poultry, improve
The immunity and the speed of growth of livestock and poultry；
2, it can be generated during livestock excrement composting being produced organic fertilizer and largely have ammonia and hydrogen sulfide frowzy,
Environmental pollution is not only caused, the loss of N element and S element is also resulted in.Bacillus flexus of the present invention and bacillus megaterium
Can the efficient oxidation ammonia, wherein some by exhaustive oxidation at nitrate nitrogen reduce N element loss, some is by oxygen
It is melted into nitrogen or NOXThe gaseous compound of odorless escapes, and the yield for the nitrite nitrogen being more toxic is few, is both able to achieve
Part fixed nitrogen can effectively remove the ammonia with stink and toxicity again；Bacillus flexus of the present invention and bacillus megaterium can
It is the sulfur-containing compounds such as elemental sulfur, thiosulfate, sulphite and sulfate by Oxidation of Hydrogen Sulfide, prevents the stream of S element
It loses, had not only been able to achieve solid sulphur but also the hydrogen sulfide gas with stink and toxicity can be effectively removed.In addition, Bacillus flexus and huge
Bacillus can also promote the raising of livestock excrement composting temperature, have facilitation to compost maturity, shorten the life of organic fertilizer
Produce the time；
3, it can be generated during handling landfill leachate or sewage and largely have ammonia and hydrogen sulfide frowzy, it will
Bacillus flexus of the present invention and bacillus megaterium are inoculated in membrane reactor, and Bacillus flexus and bacillus megaterium exist
The filler surface of membrane reactor forms advantage group and quickly generates biomembrane, biomembrane in water ammonia and hydrogen sulfide give birth to
Object oxidative degradation effectively removes the stink in landfill leachate.
Specific embodiment
Below with reference to embodiment, the present invention is described in further detail, and embodiments of the present invention are not limited thereto.
Embodiment 1: the screening and identification of bacterial strain
NH3Selective medium: sucrose 50.0g, ammonium hydroxide 10.0mL, KH2PO42.0g, MgSO4·7H2O 0.5g,FeSO4
0.1g, 1%ZnSO45.0m L, NaCl 2.0g, distilled water 1000mL, 121 DEG C of sterilizing 15min.
H2S selective medium: glucose 5.0g, K2HPO40.5g, KNO31.0g, MgCl20.5g, NaCl 0.5g,
NH4Cl 0.5g, Na2CO31.0g, FeCl20.01g, distilled water 1000mL, pH are naturally, 121 DEG C of sterilizing 15min.
The screening of deodorization bacterium:
1) pig manure 10g and percolate 10mL are weighed, 100mL NH is respectively connected to3And H2The selective medium of S, 30 DEG C,
220r/min shaking table culture replaces culture medium after 2d, draws 10mL domestication culture solution and the fresh selective medium of 100mL is added
Middle progress 2nd generation domestication, continuous acclimating 4 times.It takes pregnant solution 10mL in the triangular flask for filling 90mL sterile water, is put into Sheng
In the triangular flask for having 90mL sterile water, vibrate about 20min, as 10-1Sample diluting liquid, by 10-1、 10-2、10-3Three kinds are not
Dilution with gradient is coated on NH3On selective medium, picking colony is in NH after cultivating 14d3It is carried out on selective medium
Scribing line separation, purifies repeatedly.Pig manure 10g and percolate 10mL are weighed, is put into the triangular flask for filling 90mL sterile water, oscillation is about
20 minutes, as 10-1Sample diluting liquid, by 10-1、10-2、10-3The dilution of three kinds of different gradients is coated on H2S selectivity
On culture medium, picking colony is in H after cultivating 14d2Scribing line separation is carried out on S selective medium, is purified repeatedly.Aforesaid operations one
8 plants of bacterial strains are obtained altogether is successively denoted as Z1, Z2, Z3, S1, S2, S3, S4, S5.
2) pig manure 500g is weighed, is fitted into the beaker of 2000mL, 3% cultured bacterium solution is added and is uniformly mixed, at sealing
Manage 2h.Each strain does 3 repetitions, meanwhile, one group of blank control for only adding sterile water, any microorganism being not added is done, with preliminary
Sensory testing simultaneously records as a result, the results are shown in Table 1, wherein there is insufferable stink to be denoted as +++ ++, have very smelly
Smell be denoted as ++++, have very smelly smell be denoted as +++, have general stink be denoted as ++, have less malodor be denoted as+.
1 sensory testing's bacterial strain deodorizing effect of table
The 16SrDNA of Bacillus flexus Z2 (Bacillus flexus Z2) is identified: extracting Bacillus flexus Z2
The genomic DNA of (Bacillus flexus Z2) is expanded with bacteria 16 S rRNA genes amplification universal primer 27F/1492R
To PCR product, and send to Shanghai Mei Ji biological medicine science and technology Sheng Gong biotechnology Services Co., Ltd (Guangzhou Branch)
Sequence is carried out, sequencing result is as shown in SEQ ID NO:1.
The Physiology and biochemistry test result of Bacillus flexus Z2 (Bacillus flexus Z2): it for aerobic bacteria, can decompose
Starch utilizes glucose, Xi Mengshi citrate production alkali, V-P test, lysozyme, catalase, gelatin liquefaction and power examination
It the measurement results such as tests to be positive, it is then negative that nitrate reduction, mannose, which produce the test results such as acid and indoles,.
The 16SrDNA of bacillus megaterium Z3 (Bacillus megaterium Z3) is identified: extracting bacillus megaterium
The genomic DNA of Z3 (Bacillus megaterium Z3) expands universal primer 27F/1492R with bacteria 16 S rRNA genes
Amplification obtains PCR product, and send limited to Hai Shenggong biotechnology Services Co., Ltd Shanghai Mei Ji biological medicine science and technology
Company's (Guangzhou Branch) carries out sequence, and sequencing result is as shown in SEQ ID NO:2.
The Physiology and biochemistry test result of bacillus megaterium Z3 (Bacillus megaterium Z3): for aerobic bacteria, ovum
Yellow reaction, nitrate reduction, indole test and V-P measurement are feminine gender, and catalase, mannose produce acid test and be positive, can
Milk is peptonized, solation can be made, it can starch-splitting.
Embodiment 2: single bacterium deodorization
Single bacterium liquid culture: Bacillus flexus Z2 (Bacillus flexus Z2) is inoculated into NA culture medium, 40~
45 DEG C of culture 14d, stir evenly, are bent bacillus culture solution；By bacillus megaterium Z3 (Bacillus
Megaterium Z3) it is inoculated into NA culture medium, 30 DEG C of culture 14d are stirred evenly, obtain bacillus megaterium culture solution, two
Kind bacterial strain bacterium solution bacterium number reaches 108cfu/mL。
Test material is fresh swine excrement, is derived from Zhejiang Academy of Agricultural Science pig farm, the fresh swine excrement of 10kg is divided into
10 parts,
Control group 1: the fresh swine excrement of 1kg is placed in Flat bottom container, is shakeout then uniformly sprinkling 10mL NA culture
Liquid.The 50mL small beaker equipped with 2% boric acid solution of 20mL is placed in Flat bottom container absorbs ammonia, bottle sealing；
Control group 2: the fresh swine excrement of 1kg is placed in Flat bottom container, is shakeout then uniformly sprinkling 10mL NA culture
Liquid.The 50mL small beaker equipped with 20mL zinc ammonium complex salt solution is placed in Flat bottom container absorbs hydrogen sulfide, bottle sealing；
Test group 1: the fresh swine excrement of 1kg is placed in Flat bottom container, and it is above-mentioned curved to be shakeout then uniform sprinkling 10mL
Bent bacillus culture solution.The 50mL small beaker equipped with 2% boric acid solution of 20mL is placed in Flat bottom container absorbs ammonia, bottleneck
Sealing；
Test group 2: the fresh swine excrement of 1kg is placed in Flat bottom container, and it is above-mentioned curved to be shakeout then uniform sprinkling 10mL
Bent bacillus culture solution.The 50mL small beaker equipped with 20mL zinc ammonium complex salt solution is placed in Flat bottom container absorbs hydrogen sulfide, bottle
Mouth sealing；
Test group 3: the fresh swine excrement of 1kg is placed in Flat bottom container, and it is above-mentioned huge to be shakeout then uniform sprinkling 10mL
Bacterium anthracoides culture solution.The 50mL small beaker equipped with 2% boric acid solution of 20mL is placed in Flat bottom container absorbs ammonia, bottleneck
Sealing；
Test group 4: the fresh swine excrement of 1kg is placed in Flat bottom container, and it is above-mentioned huge to be shakeout then uniform sprinkling 10mL
Bacterium anthracoides culture solution.The 50mL small beaker equipped with 20mL zinc ammonium complex salt solution is placed in Flat bottom container absorbs hydrogen sulfide, bottle
Mouth sealing；
Test group 5: the fresh swine excrement of 1kg is placed in Flat bottom container, is shakeout and then uniformly sprayed above-mentioned bending bud
Spore bacillus dry powder, bacterial strain bacterium number is 10 in dry powder8cfu/mL.The 50mL that 2% boric acid solution of 20mL is housed is placed in Flat bottom container
Small beaker absorbs ammonia, bottle sealing；
Test group 6: the fresh swine excrement of 1kg is placed in Flat bottom container, is shakeout and then uniformly sprayed above-mentioned bending bud
Spore bacillus dry powder, bacterial strain bacterium number is 10 in dry powder8cfu/mL.The 50mL that 20mL zinc ammonium complex salt solution is housed is placed in Flat bottom container
Small beaker absorbs hydrogen sulfide, bottle sealing；
Test group 7: the fresh swine excrement of 1kg is placed in Flat bottom container, is shakeout and then uniformly sprayed above-mentioned huge bud
Spore bacillus dry powder, bacterial strain bacterium number is 10 in dry powder8cfu/mL.The 50mL that 2% boric acid solution of 20mL is housed is placed in Flat bottom container
Small beaker absorbs ammonia, bottle sealing；
Test group 8: the fresh swine excrement of 1kg is placed in Flat bottom container, is shakeout and then uniformly sprayed above-mentioned huge bud
Spore bacillus dry powder, bacterial strain bacterium number is 10 in dry powder8cfu/mL.The 50mL that 20mL zinc ammonium complex salt solution is housed is placed in Flat bottom container
Small beaker absorption hydrogen sulfide, bottle sealing,
It measures ammonia level in above-mentioned group Flat bottom container and uses national food safety standard " GB 5009.228-2016 food
The measurement of Volatile Base Nitrogen in product " in microdiffusion, respectively in 1,3,7,14,21d, in above-mentioned group Flat bottom container
Ammonia level is measured, and each period is detected 3 times, is averaged and calculates ammonia removal rate, measurement result such as 2 institute of table
Show；
Hydrogen sulfide content in above-mentioned group Flat bottom container is measured to produce just using Shenzhen Ji Shunan Science and Technology Ltd.
The formula H2S detector of taking is detected, respectively in 1,3,7,14,21d, to hydrogen sulfide content in above-mentioned group Flat bottom container into
Row measurement, each period are detected 3 times, are averaged and calculate hydrogen sulfide removal rate, measurement result is as shown in table 3.
Table 2 Bacillus flexus Z2 and bacillus megaterium Z3 detects the removal rate of ammonia
Table 3 Bacillus flexus Z2 and bacillus megaterium Z3 detects the removal rate of hydrogen sulfide
Referring specifically to table 2, after Bacillus flexus culture solution is to fresh swine manure treatment 1d, except ammonia rate reaches 44.59%；Place
It manages after 3 d except ammonia rate reaches 81.34%；Except ammonia rate reaches 91.67% after processing 7d, it can thus be appreciated that Bacillus flexus culture solution
The ammonia that fresh pig manure generates can be removed substantially.Except ammonia rate reaches 93.67% after processing 14d；Except ammonia rate reaches after processing 21d
93.91%, it can thus be appreciated that Bacillus flexus forms advantage group in pig manure, pig manure can be maintained to be in substantially not for a long time
Escape ammonia state.In addition during Bacillus flexus culture solution is to fresh swine manure treatment, fresh pig manure generates high
Warm (45 DEG C~58 DEG C) but remain to maintain not escaping out ammonia state substantially in Flat bottom container, it is high to illustrate that Bacillus flexus adapts to
Temperature, can be applied to that livestock excrement composting deodorization etc. needs to adapt to high temperature needs field of deodorization.Bacillus megaterium culture solution is to new
After fresh pig dung handles 1d, except ammonia rate reaches 33.45%；Except ammonia rate reaches 76.38% after processing 3d, it follows that huge gemma
Bacillus culture solution is for fresh pig manure except ammonia effect will be inferior to Bacillus flexus culture solution.But except ammonia rate reaches after processing 7d
90.21%；Except ammonia rate reaches 93.57% after processing 14d；Except ammonia rate reaches 94.42% after processing 21d, as long as it follows that place
The reason time be longer than 7d bacillus megaterium culture solution for fresh pig manure removing except ammonia effect and Bacillus flexus culture solution
Ammonia effect is suitable.By the data of test group 5 and test group 7 it is found that processing initial stage, Bacillus flexus dry powder and huge gemma bar
Bacterium dry powder except ammonia effect to be inferior to Bacillus flexus culture solution and bacillus megaterium culture solution except ammonia effect, but as long as
The processing time is longer than 14d, dry powder and culture solution except ammonia effect it is suitable.This is because dry powder handles fresh pig manure, in dry powder
Strain has activation stage.
Referring specifically to table 3, after Bacillus flexus culture solution is to fresh swine manure treatment 1d, vulcanisation hydrogen rate reaches
85.71%；Except ammonia rate reaches 100.00% after processing 3d, it can thus be appreciated that Bacillus flexus culture solution can generate fresh pig manure
Hydrogen sulfide all remove.Except ammonia rate reaches 100.00% after processing 7d；Except ammonia rate reaches 100.00% after processing 14d；Processing
Except ammonia rate reaches 100.00% after 21d, it can thus be appreciated that Bacillus flexus forms advantage group in pig manure, can tie up for a long time
Hold pig manure be in do not escape out hydrogen sulfide state.By table 2 and table 3 it is found that Bacillus flexus and bacillus megaterium are either dry
Powder or culture solution can carry out deodorization to fresh pig manure well, and two kinds of bacterium just adapt to hot environment, can be applied to raise
What poultry manure compost deodorizing etc. needed to adapt to high temperature needs field of deodorization.
Embodiment 3: Mixed Microbes deodorization
Test material is fresh swine excrement, and the fresh swine excrement of 10kg is divided into 10 parts,
Test group 9: by the Bacillus flexus culture solution cultivated in embodiment 1 and bacillus megaterium culture solution by
Mixed-culture medium 1 is mixed to get according to 1:1.The fresh swine excrement of 1kg is placed in Flat bottom container, uniformly sprinkling is shakeout then
The above-mentioned mixed-culture medium 1 of 10mL.The 50mL small beaker equipped with 2% boric acid solution of 20mL is placed in Flat bottom container absorbs ammonia,
Bottle sealing；
Test group 10: by the Bacillus flexus culture solution cultivated in embodiment 1 and bacillus megaterium culture solution
Mixed-culture medium 1 is mixed to get according to 1:1.The fresh swine excrement of 1kg is placed in Flat bottom container, uniformly spray is shakeout then
Spill the above-mentioned mixed-culture medium 1 of 10mL.The 50mL small beaker equipped with 20mL zinc ammonium complex salt solution is placed in Flat bottom container absorbs vulcanization
Hydrogen, bottle sealing；
Test group 11: by the Bacillus flexus culture solution cultivated in embodiment 1 and bacillus megaterium culture solution
Mixed-culture medium 2 is mixed to get according to 2:3.The fresh swine excrement of 1kg is placed in Flat bottom container, uniformly spray is shakeout then
Spill the above-mentioned mixed-culture medium 2 of 10mL.The 50mL small beaker absorbing ammonia that 2% boric acid solution of 20mL is housed is placed in Flat bottom container
Gas, bottle sealing；
Test group 12: by the Bacillus flexus culture solution cultivated in embodiment 1 and bacillus megaterium culture solution
Mixed-culture medium 2 is mixed to get according to 2:3.The fresh swine excrement of 1kg is placed in Flat bottom container, uniformly spray is shakeout then
Spill the above-mentioned mixed-culture medium 2 of 10mL.The 50mL small beaker equipped with 20mL zinc ammonium complex salt solution is placed in Flat bottom container absorbs vulcanization
Hydrogen, bottle sealing；
Test group 13: by the Bacillus flexus culture solution cultivated in embodiment 1 and bacillus megaterium culture solution
Mixed-culture medium 3 is mixed to get according to 3:2.The fresh swine excrement of 1kg is placed in Flat bottom container, uniformly spray is shakeout then
Spill the above-mentioned mixed-culture medium 3 of 10mL.The 50mL small beaker absorbing ammonia that 2% boric acid solution of 20mL is housed is placed in Flat bottom container
Gas, bottle sealing；
Test group 14: by the Bacillus flexus culture solution cultivated in embodiment 1 and bacillus megaterium culture solution
Mixed-culture medium 3 is mixed to get according to 3:2.The fresh swine excrement of 1kg is placed in Flat bottom container, uniformly spray is shakeout then
Spill the above-mentioned mixed-culture medium 3 of 10mL.The 50mL small beaker equipped with 20mL zinc ammonium complex salt solution is placed in Flat bottom container absorbs vulcanization
Hydrogen, bottle sealing；
It measures ammonia level in above-mentioned group Flat bottom container and uses national food safety standard " GB 5009.228-2016 food
The measurement of Volatile Base Nitrogen in product " in microdiffusion, respectively in 1,3,7,14,21d, in above-mentioned group Flat bottom container
Ammonia level is measured, and each period is detected 3 times, is averaged and calculates ammonia removal rate, measurement result such as 4 institute of table
Show；
Hydrogen sulfide content in above-mentioned group Flat bottom container is measured to produce just using Shenzhen Ji Shunan Science and Technology Ltd.
The formula H2S detector of taking is detected, respectively in 1,3,7,14,21d, to hydrogen sulfide content in above-mentioned group Flat bottom container into
Row measurement, each period are detected 3 times, are averaged and calculate hydrogen sulfide removal rate, measurement result is as shown in table 5.
4 Mixed Microbes of table detect the removal rate of ammonia
5 Mixed Microbes of table detect the removal rate of hydrogen sulfide
Referring specifically to table 4, after mixed-culture medium 1 is to fresh swine manure treatment 1d, except ammonia rate reaches 58.11%；After handling 3d
Except ammonia rate reaches 85.71%；Except ammonia rate reaches 92.99% after processing 7d；Except ammonia rate reaches 95.42% after processing 14d, processing
Except ammonia rate reaches 95.91% after 21d.After mixed-culture medium 2 is to fresh swine manure treatment 1d, except ammonia rate reaches 50.34%；Handle 3d
Afterwards except ammonia rate reaches 82.22%；Except ammonia rate reaches 91.53% after processing 7d；Except ammonia rate reaches 93.57% after processing 14d, processing
Except ammonia rate reaches 94.43% after 21d.After mixed-culture medium 3 is to fresh swine manure treatment 1d, except ammonia rate reaches 43.58%；Handle 3d
Afterwards except ammonia rate reaches 80.76%；Except ammonia rate reaches 88.36% after processing 7d；Except ammonia rate reaches 93.57% after processing 14d, processing
Except ammonia rate reaches 93.91% after 21d.It follows that in above-mentioned Bacillus flexus culture solution and bacillus megaterium culture solution
Mixed proportion in 1:1 mixed effect it is best.Referring specifically to table 2, table 4, Bacillus flexus culture solution is to fresh swine manure treatment
After 1d, except ammonia rate reaches 44.59%；Except ammonia rate reaches 81.34% after processing 3d；Except ammonia rate reaches 91.67% after processing 7d；
Except ammonia rate reaches 93.67% after processing 14d；Except ammonia rate reaches 93.91% after processing 21d.Bacillus megaterium culture solution is to new
After fresh pig dung handles 1d, except ammonia rate reaches 33.45%；Except ammonia rate reaches 76.38% after processing 3d；Except ammonia rate reaches after processing 7d
To 90.21%；Except ammonia rate reaches 93.57% after processing 14d；Except ammonia rate reaches 94.42% after processing 21d.It follows that bending
Bacillus culture solution will be since single bacterium liquid be except ammonia is imitated according to the ammonia effect of removing that 1:1 is mixed with bacillus megaterium culture solution
Fruit.
Referring specifically to table 5, after mixed-culture medium 1 and mixed-culture medium 2 are to fresh swine manure treatment 1d, vulcanisation hydrogen rate just reaches
To 100.00%；3d, 7d, 14d and 21d are always maintained at vulcanisation hydrogen rate 100.00% later, it can thus be appreciated that bending gemma
Bacillus mixes according to 1:1 and 2:3 with bacillus megaterium and can quickly, thoroughly remove the hydrogen sulfide that fresh pig manure generates.
Although Bacillus flexus is mixed with bacillus megaterium according to 3:2 just reach 85.71%, 3d when handling 1d after just reach
100.00%, vulcanisation hydrogen effect is also fine.Referring specifically to table 3, Bacillus flexus culture solution is to fresh swine manure treatment 1d
Afterwards, vulcanisation hydrogen rate reaches 85.71%；Except ammonia rate reaches 100.00% after processing 3d, ammonia rate is removed after processing 7d and is reached
100.00%；Except ammonia rate reaches 100.00% after processing 14d；Except ammonia rate reaches 100.00% after processing 21d.Bacillus megaterium
After culture solution is to fresh swine manure treatment 1d, vulcanisation hydrogen rate reaches 71.43%；Except ammonia rate reaches 100.00% after processing 3d, place
Except ammonia rate reaches 100.00% after reason 7d；Except ammonia rate reaches 100.00% after processing 14d；Except ammonia rate reaches after processing 21d
100.00%.By table 3 and table 5 it is found that either Bacillus flexus and bacillus megaterium single bacterium liquid or two kinds of bacterium solutions is pressed
It is mixed according to 1:1,2:3,3:2, it can be well by the hydrogen sulfide removal in pig manure.Except the present embodiment enumerate it is several specific
Ratio, Bacillus flexus Z2 and bacillus megaterium Z3 matched according to the ratio of 0~100:0~100, equal energy
Well by the ammonia and hydrogen sulfide removal in pig manure, other ratios are herein without enumerating.
Embodiment 4:
Bacillus flexus Z2 is grown in culture medium of the ammonium chloride as only nitrogen source and deamination ability quantitative detection:
Culture medium prescription of the ammonium chloride as only nitrogen source: sodium succinate 2.5g, trisodium citrate dihydrate 2.5g, NH4Cl
0.0636 g, K2HPO41g, KH2PO41g, MgSO4·7H2O 0.2g, pH 7.4 is settled to 1000mL, packing to 250mL tri-
In the bottle of angle, every bottle of 100mL.121 DEG C, 20min, autoclave sterilization.Compounded carbons 1% after addition aseptic filtration.Composite carbon
Source: D-Glucose 6.9g, D-Fructose 6.9g, D- lactose 6.9g, 90% lactic acid 6.4mL, mannitol 7g, sodium acetate 9.5g, glycerol
6.3mL, dehydrated alcohol 7mL, salicylic acid 4.6g, sodium benzoate 4.8g are dissolved in 500mL water, 7.4,0.22 μm of membrane filtration of pH
Degerming.
Bacillus flexus Z2 is seeded in above-mentioned culture medium, 30 DEG C, 120rpm shaking table culture, respectively culture 0,8,
12,16,19,22,25, after 28h, sampling, centrifuging and taking supernatant, quantitative detection ammonium ion concentration (Na's reagent).Measurement knot
Fruit is as shown in table 6.
Bacillus megaterium Z3 is grown in culture medium of the ammonium sulfate as only nitrogen source and deamination ability quantitative detection:
Culture medium prescription of the ammonium chloride as only nitrogen source: sodium succinate 2.5g, trisodium citrate dihydrate 2.5g, (NH4)2SO40.0916g, K2HPO41g, KH2PO41g, MgSO4·7H2O 0.2g, pH 7.4 is settled to 1000mL, packing to 250
In mL triangular flask, every bottle of 100mL.121 DEG C, 20min, autoclave sterilization.Compounded carbons 1% after addition aseptic filtration.It is multiple
Close carbon source: D-Glucose 6.9g, D-Fructose 6.9g, D- lactose 6.9g, 90% lactic acid 6.4mL, mannitol 7g, sodium acetate 9.5g,
Glycerol 6.3mL, dehydrated alcohol 7mL, salicylic acid 4.6g, sodium benzoate 4.8g are dissolved in 500mL water, 7.4,0.22 μm of pH filter
Film filtration sterilization.
Bacillus megaterium Z3 is seeded in above-mentioned culture medium, 30 DEG C, 120rpm shaking table culture, respectively culture 0,8,
12,16,19,22,25, after 28h, sampling, centrifuging and taking supernatant, quantitative detection ammonium ion concentration (Na's reagent).Measurement knot
Fruit is as shown in table 7.
6 Bacillus flexus Z2 deamination ability quantitative detection of table
7 bacillus megaterium Z3 deamination ability quantitative detection of table
Referring specifically to table 6 and table 7, initial concentration in culture medium can be 25mg/L when cultivating 22h by Bacillus flexus
Ammonium ion degradation completely, by blood counting chamber count find its bacterium number be 6.9 × 107A/mL；Bacillus megaterium exists
When cultivating 25h, the ammonium ion that initial concentration in culture medium is 25mg/L can be degraded completely, be counted and sent out by blood counting chamber
Its existing bacterium number is 8.5 × 108A/mL.It follows that Bacillus flexus and bacillus megaterium are to the ammonium ion in solution
There is good degradation effect and strain growth is in order.
Embodiment 5:
Culture medium prescription: peptone 10g, beef extract 5g, glucose 5g, agar powder 20g are settled to 1000mL, and packing is extremely
In 250mL triangular flask, every bottle of 100mL.121 DEG C, 20min, autoclave sterilization.The preparation of 0.05MPBS: solution A: second liquid 1:4
Ratio prepare, solution A 9.465g sodium dihydrogen phosphate adds water to 1000mL, and second liquid 9.27g hydrogen dipotassium of recasting adds water to 1000mL.
1) sample pretreatment: Bacillus flexus Z2 bacterium powder 5g is weighed in 500mL band bead shaking flask, is then shaken at this
90mL sterile water is added in bottle, shaking flask is placed on 120rpm on shaking table, shakes under the conditions of 30 DEG C uniformly, is finally placed in shaking flask,
30 DEG C of water-bath inside holdings are stand-by, using count plate and record the bacterium number in shaking flask；
2) dilution of sample: being separately added into 9mL PBS into 20 sterile test tubes, label sample number and dilution times on test tube
Digital sample, operation carry out on superclean bench.1.0mL sample suspension is drawn in first test tube, then from first test tube
Middle absorption 1.0mL sample suspension successively carries out 10 times and is diluted to the 6th test tube (the 6th test tube need to be done in second test tube
Repeat twice), then be added separately in remaining 13 test tubes from two the 6th test tubes points of 13 each 1.0mL that draw.(every time
When sample solution dilutes, the test tube of sample-adding need to shake at least 30~60s on miniature vortex mixed instrument, then be grasped next time
Make.)；
3) pyrometry: taking 12 test tubes to be put into water-bath in 85 DEG C of water-baths from last 13 test tubes mixed,
It is cooling (until 60min) to take out a test tube by every 5min after equalized temperature.Then every test tube exists again again respectively
It is uniformly mixed on whirlpool mixed instrument.13rd test tube is not heat-treated after adding sample suspension.With 1.0mL Sterile pipette from this ten
1.0mL sample solution accurately to be drawn in three test tubes respectively to be added in a set of sterile petri dish, every test tube makees 4 culture dishes,
That is 4 repetitions.Above-mentioned culture medium is kept the temperature in water-bath to 85 DEG C, the above-mentioned culture medium of 9mL is poured into each culture dish, and
Bacteria suspension is uniformly mixed with culture medium rapidly, then culture dish is placed in constant incubator after cultivating 48h using plate meter
Number method counts and records viable count；
4) it calculates: the bacterium number N=K*10n/M of Bacillus flexus Z2 in sample
K is the average value that 4 culture dish viable counts of 1.0mL are drawn in every test tube of 13 test tubes in formula；
10n is the extension rate (n=8) of sample；
M is the weighed quality of sample, and unit is gram (M=5)
The death rate (Q1)=(N0-N1)/N0*100%
Q1 is the death rate of the test tube sample of water-bath 5min in 13 test tubes in formula；
N0 is the viable count of the test tube sample of not water-bath in 13 test tubes；
N1 is the viable count of the test tube sample of water-bath 5min in 13 test tubes；
Q2~Q12 is ibid calculated below, and calculated result is as shown in table 8.
Bacillus flexus Z2 bacterium powder is replaced with into bacillus megaterium Z3 bacterium powder and repeats above-mentioned experiment, calculated result such as table
Shown in 9.
The detection of 8 Bacillus flexus Z2 high temperature resistance of table
The detection of 9 bacillus megaterium Z3 high temperature resistance of table
Referring specifically to table 8 and table 9, in 85 DEG C of hot environment, Bacillus flexus Z2 and bacillus megaterium Z3's
The death rate is lower, illustrates that Bacillus flexus Z2 and bacillus megaterium Z3 can high temperature resistants.
Embodiment 6:
Bacillus flexus Z2 (Bacillus flexus Z2) is in sulfur-bearing (S2-) grow in culture medium and desulphurizing ability is fixed
Amount detection:
Sulfur-bearing (S2-) culture medium prescription: Na2S2O3·5H2O 600mg/L,NaHCO3 1.0g/L,KH2PO4 1.0g/L,
K2HPO4 1.0g/L,MgCl2·6H2O 0.8g/L,NH4Cl 0.4g/L, trace element solution 1mL；Finally, pH value is adjusted to 7.0.
121 DEG C, 20min, autoclave sterilization.
Bacillus flexus Z2 is seeded in above-mentioned culture medium, 30 DEG C, 120rpm shaking table culture, respectively culture 0,
0.5,1,1.5,2,2.5,3, after 3.5h, sampling, centrifuging and taking supernatant, quantitative detection sulfide concentration simultaneously calculates sulfide removal
Rate.Measurement result is as shown in table 6.
Bacillus megaterium Z3 is in sulfur-bearing (S2-) grow in culture medium and desulphurizing ability quantitative detection:
Sulfur-bearing (S2-) culture medium prescription: Na2S2O3·5H2O 600mg/L,NaHCO3 1.0g/L,KH2PO4 1.0g/L,
K2HPO4 1.0 g/L,MgCl2·6H2O 0.8g/L,NH4Cl 0.4g/L, trace element solution 1mL；Finally, pH value is adjusted to
7.0.121 DEG C, 20min, autoclave sterilization.
Bacillus megaterium Z3 is seeded in above-mentioned culture medium, 30 DEG C, 120rpm shaking table culture, respectively culture 0,
0.5,1,1.5,2,2.5,3, after 3.5h, sampling, centrifuging and taking supernatant, quantitative detection sulfide concentration simultaneously calculated kind to above-mentioned culture
In base, 30 DEG C, 120rpm shaking table culture, respectively culture 0,0.5,1,1.5,2,2.5,3, after 3.5h, sampling, in centrifuging and taking
Clearly, quantitative detection sulfide concentration and conversion ratio and concentration of hydrogen sulfide are calculated.Measurement result is as shown in table 7.
Removal rate of the 10 Bacillus flexus Z2 of table to sulfide
Removal rate of the 11 bacillus megaterium Z3 of table to sulfide
Referring specifically to table 10 and table 11, it is known that Bacillus flexus and bacillus megaterium are to sulfide with good
Removal effect, and being capable of removing sulfide quickly and efficiently.Therefore Bacillus flexus and huge gemma bar of the invention
Bacterium is suitable for the object to be processed such as landfill leachate of complicated component.
Embodiment 7:
Test material is landfill leachate, and landfill leachate is derived from Hangzhou refuse landfill, and 12kg landfill leachate is equal
It is divided into 4 parts,
Control group 3: 4kg landfill leachate is poured into Flat bottom container, then 10mL NA culture solution is also added flat
In container, it is uniformly mixed.The 50mL small beaker equipped with 2% boric acid solution of 20mL is placed in Flat bottom container and absorbs ammonia, and bottleneck is close
Envelope；
Control group 4: 4kg landfill leachate is poured into Flat bottom container, then 10mL NA culture solution is also added flat
In container, it is uniformly mixed.The 50mL small beaker equipped with 20mL zinc ammonium complex salt solution is placed in Flat bottom container absorbs hydrogen sulfide, bottleneck
Sealing；
Test group 15: by the Bacillus flexus culture solution cultivated in embodiment 1 and bacillus megaterium culture solution
Mixed-culture medium 4 is mixed to get according to 1:1.4kg landfill leachate is poured into Flat bottom container, then 10mL is mixed and is trained
Nutrient solution 4 is also added in Flat bottom container, is uniformly mixed.The small burning of 50mL that 2% boric acid solution of 20mL is housed is placed in Flat bottom container
Cup absorbs ammonia, bottle sealing；
Test group 16: by the Bacillus flexus culture solution cultivated in embodiment 1 and bacillus megaterium culture solution
Mixed-culture medium 4 is mixed to get according to 1:1.4kg landfill leachate is poured into Flat bottom container, then 10mL is mixed and is trained
Nutrient solution 4 is also added in Flat bottom container, is uniformly mixed.The small burning of 50mL that 20mL zinc ammonium complex salt solution is housed is placed in Flat bottom container
Cup absorbs hydrogen sulfide, bottle sealing.
It measures ammonia level in above-mentioned group Flat bottom container and uses national food safety standard " GB 5009.228-2016 food
The measurement of Volatile Base Nitrogen in product " in microdiffusion, respectively in 1,3,7,14,21d, in above-mentioned group Flat bottom container
Ammonia level is measured, and each period is detected 3 times, is averaged and calculates ammonia removal rate, measurement result such as 12 institute of table
Show.
Hydrogen sulfide content in above-mentioned group Flat bottom container is measured to produce just using Shenzhen Ji Shunan Science and Technology Ltd.
The formula H2S detector of taking is detected, and respectively in 1d, 3d, 7d, 14d, 21d, is contained to hydrogen sulfide in above-mentioned group Flat bottom container
Amount is measured, and each period is detected 3 times, is averaged and is calculated hydrogen sulfide removal rate, measurement result is as shown in table 13.
Ammonia removal rate measures in 12 landfill leachate of table
Hydrogen sulfide removal rate measures in 13 landfill leachate of table
Referring specifically to table 12, mixed-culture medium 4 is when handling landfill leachate 7d, except ammonia rate reaches 91.99%, later
14d and 21d, mixed-culture medium 4 respectively reach 92.35% and 93.63% to the ammonia rate of removing of landfill leachate.It follows that mixed
Culture solution 4 is closed to landfill leachate except ammonia effect is good, and in the landfill leachate shape of the strain in mixed-culture medium 4
At advantage group, landfill leachate can be maintained to be in the state for not escaping out ammonia substantially for a long time, may be used on practical rubbish and seep
In the deodorization work of filtrate.Referring specifically to table 13, when handling landfill leachate 14d, vulcanisation hydrogen rate reaches mixed-culture medium 4
100.00%, 21d later, mixed-culture medium 4 reach 100.00% to the vulcanisation hydrogen rate of landfill leachate.It follows that
Mixed-culture medium 4 can maintain landfill leachate to be in the vulcanisation hydrogen effect of landfill leachate for a long time and not escape out vulcanization very much
The state of hydrogen has very high application value in the deodorization work of landfill leachate.
Bacillus flexus Z2 and bacillus megaterium Z3, which is also applied to other, to be needed in the object to be processed of biological deodorizing,
The present invention is herein without enumerating.
The embodiments of the present invention have been described in detail above, but content is only the preferred embodiment of the present invention,
It should not be considered as limiting the scope of the invention.Any changes and modifications in accordance with the scope of the present application,
It should still be within the scope of the patent of the present invention.
Sequence table
<110>Zhejiang Academy of Agricultural Science
<120>a kind of purposes of microbial deoderizer
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1421
<212> DNA
<213>Bacillus flexus Z2 (Bacillus flexus Z2)
<400> 1
gcagtcgagc gaactgatta gaagcttgct tctatgacgt tagcggcgga cgggtgagta 60
acacgtgggc aacctgcctg taagactggg ataacttcgg gaaaccgaag ctaataccgg 120
ataggatctt ctccttcatg ggagatgatt gaaagatggt ttcggctatc acttacagat 180
gggcccgcgg tgcattagct agttggtgag gtaacggctc accaaggcaa cgatgcatag 240
ccgacctgag agggtgatcg gccacactgg gactgagaca cggcccagac tcctacggga 300
ggcagcagta gggaatcttc cgcaatggac gaaagtctga cggagcaacg ccgcgtgagt 360
gatgaaggct ttcgggtcgt aaaactctgt tgttagggaa gaacaagtac aagagtaact 420
gcttgtacct tgacggtacc taaccagaaa gccacggcta actacgtgcc agcagccgcg 480
gtaatacgta ggtggcaagc gttatccgga attattgggc gtaaagcgcg cgcaggcggt 540
ttcttaagtc tgatgtgaaa gcccacggct caaccgtgga gggtcattgg aaactgggga 600
acttgagtgc agaagagaaa agcggaattc cacgtgtagc ggtgaaatgc gtagagatgt 660
ggaggaacac cagtggcgaa ggcggctttt tggtctgtaa ctgacgctga ggcgcgaaag 720
cgtggggagc aaacaggatt agataccctg gtagtccacg ccgtaaacga tgagtgctaa 780
gtgttagagg gtttccgccc tttagtgctg cagctaacgc attaagcact ccgcctgggg 840
agtacggtcg caagactgaa actcaaagga attgacgggg gcccgcacaa gcggtggagc 900
atgtggttta attcgaagca acgcgaagaa ccttaccagg tcttgacatc ctctgacaac 960
tctagagata gagcgttccc cttcggggga cagagtgaca ggtggtgcat ggttgtcgtc 1020
agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaaccctt gatcttagtt 1080
gccagcattc agttgggcac tctaaggtga ctgccggtga caaaccggag gaaggtgggg 1140
atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgctac aatggatggt 1200
acaaagggct gcaagaccgc gaggtcaagc caatcccata aaaccattct cagttcggat 1260
tgtaggctgc aactcgccta catgaagctg gaatcgctag taatcgcgga tcagcatgcc 1320
gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag agtttgtaac 1380
acccgaagtc ggtggagtaa ccgtaaggag ctagccgcct a 1421
<210> 2
<211> 1415
<212> DNA
<213>bacillus megaterium Z3 (Bacillus megaterium Z3)
<400> 2
gttacgactt ggttaccttg ttacgacttg gttaccttgt tacgacttgg ttaccttgtt 60
acgacttggt taccttgtta cgacttggtt accttgttac gacttggtta ccttgttacg 120
acttggttac cttgttacga cagttgcagc ctgtatccga actgataatg gttttatggg 180
attggcttga cctcgcggtc ttgcagccct ttgtaccatc cattgtagca cgtgtgtagc 240
ccaggtcata aggggcatga tgatttgacg tcatccccac cttcctccgg tttgtcaccg 300
gcagtcacct tagagtgccc aactaaatgc tggcaactaa gatcaagggt tgcgctcgtt 360
gcgggactta acccaacatc tcacgacacg agctgacgac aaccatgcac cacctgtcac 420
tctgtccccc gaaggggaac gctctatctc tagagttgtc agaggatgtc aagacctggt 480
aaggttcttc gcgttgcttc aaattaaacc acatgctcca ccgcttgtgc gggcccccgt 540
caattccttt gagtttcagt cttgcgaccg tactccccag gcggagtgct taatgcgtta 600
gctgcagcac taaagggcgg aaaccctcta acacttagca ctcatcgttt acggcgtgga 660
ctaccagggt atctaatcct gtttgctccc cacgctttcg cgcctcagcg tcagttacag 720
accaaaaagc cgccttcgcc actggtgttc ctccacatct ctacgcattt caccgctaca 780
cgtggaattc cgcttttctc ttctgcactc aagttcccca gtttccaatg accctccacg 840
gttgagccgt gggctttcac atcagactta agaaaccgcc tgcgcgcgct ttacgcccaa 900
taattccgga taacgcttgc cacctacgta ttaccgcggc tgctggcacg tagttagccg 960
tggctttctg gttaggtacc gtcaaggtac aagcagttac tcttgtactt gttcttccct 1020
aacaacagag ttttacgacc cgaaagcctt catcactcac gcggcgttgc tccgtcagac 1080
tttcgtccat tgcggaagat tccctactgc tgcctcccgt aggagtctgg gccgtgtctc 1140
agtcccagtg tggccgatca ccctctcagg tcggctatgc atcgttgcct tggtgagccg 1200
ttacctcacc aactagctaa tgcaccgcgg gcccatctgt aagtgatagc cgaaaccatc 1260
tttcaatcat ctcccatgaa ggagaagatc ctatccggta ttagcttcgg tttcccgaag 1320
tcatcccagt cttacaggca cgttgcccac gtgttactcc cccgtccgcc gctaacgtca 1380
tagaagcaag ctgagtaatc gatcaaactc tgagc 1415
Claims (7)
1. a kind of microbial deoderizer, it is characterised in that be deposit number containing deposit number be CCTCC NO:M 2018660
Bacillus megaterium Z3 (Bacillus megaterium Z3) or/and its culture or/and its machining object.
2. a kind of microbial deoderizer according to claim 1, it is characterised in that also contain CCTCC NO:M
2018659 Bacillus flexus Z2 (Bacillus flexus Z2) or/and its culture or/and its machining object.
3. a kind of microbial deoderizer according to claim 1, it is characterised in that the Bacillus flexus Z2
(Bacillus flexus Z2) resistance to 85 DEG C high temperature.
4. a kind of microbial deoderizer according to claim 2, it is characterised in that the bacillus megaterium Z3
(Bacillus megaterium Z3) resistance to 85 DEG C high temperature.
5. a kind of microbial deoderizer according to claim 2, it is characterised in that Bacillus flexus Z2 (Bacillus
Flexus Z2) and bacillus megaterium Z3 (Bacillus megaterium Z3) mixed proportion be 0~100:0~100.
6. a kind of purposes of microbial deoderizer, it is characterised in that remove a kind of microorganism described in claims 1 or 2 or 5
Smelly microbial inoculum is used for biological deodorizing.
7. a kind of purposes of microbial deoderizer according to claim 6, it is characterised in that Bacillus flexus Z2
(Bacillus flexus Z2) dry powder or bacillus megaterium Z3 (Bacillus megaterium Z3) dry powder or bending bud
Spore bacillus Z2 (Bacillus flexus Z2) culture solution or bacillus megaterium Z3 (Bacillus megaterium Z3) training
Nutrient solution or Bacillus flexus Z2 (Bacillus flexus Z2) and bacillus megaterium Z3 (Bacillus megaterium
Z3) the mixed liquor being mixed to get according to the ratio uniform of 0~100:0~100 imposes in the object to be processed for needing biological deodorizing.
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