CN101603019A - A kind of high-inorganic nitrogen-sourced biological compound fertilizer and preparation method thereof - Google Patents
A kind of high-inorganic nitrogen-sourced biological compound fertilizer and preparation method thereof Download PDFInfo
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- CN101603019A CN101603019A CNA200910089775XA CN200910089775A CN101603019A CN 101603019 A CN101603019 A CN 101603019A CN A200910089775X A CNA200910089775X A CN A200910089775XA CN 200910089775 A CN200910089775 A CN 200910089775A CN 101603019 A CN101603019 A CN 101603019A
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- microbial inoculum
- composite fertilizer
- inorganic nitrogen
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 89
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 150000001875 compounds Chemical class 0.000 title claims abstract description 6
- 239000002131 composite material Substances 0.000 claims abstract description 53
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 31
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 15
- 241000186660 Lactobacillus Species 0.000 claims abstract description 14
- 241000187759 Streptomyces albus Species 0.000 claims abstract description 14
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 14
- 241000235395 Mucor Species 0.000 claims abstract description 12
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 10
- 239000002068 microbial inoculum Substances 0.000 claims description 51
- 238000000855 fermentation Methods 0.000 claims description 20
- 230000004151 fermentation Effects 0.000 claims description 20
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 claims description 19
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 14
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 13
- 239000004202 carbamide Substances 0.000 claims description 13
- 238000005507 spraying Methods 0.000 claims description 13
- 239000002054 inoculum Substances 0.000 claims description 9
- 239000000463 material Substances 0.000 claims description 9
- 239000011368 organic material Substances 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims description 3
- 230000004048 modification Effects 0.000 claims description 3
- 238000012986 modification Methods 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 12
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 6
- 238000002474 experimental method Methods 0.000 abstract description 2
- 241000499436 Brassica rapa subsp. pekinensis Species 0.000 description 17
- 235000010149 Brassica rapa subsp chinensis Nutrition 0.000 description 15
- 235000000536 Brassica rapa subsp pekinensis Nutrition 0.000 description 15
- 241000196324 Embryophyta Species 0.000 description 14
- 230000009849 deactivation Effects 0.000 description 11
- 230000001580 bacterial effect Effects 0.000 description 10
- 238000012545 processing Methods 0.000 description 8
- 238000004519 manufacturing process Methods 0.000 description 7
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 239000008272 agar Substances 0.000 description 5
- 238000011081 inoculation Methods 0.000 description 5
- 244000005700 microbiome Species 0.000 description 5
- 229910052700 potassium Inorganic materials 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 4
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 4
- 210000003608 fece Anatomy 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000010871 livestock manure Substances 0.000 description 4
- 229910052698 phosphorus Inorganic materials 0.000 description 4
- 239000011591 potassium Substances 0.000 description 4
- 238000011218 seed culture Methods 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 3
- 230000003321 amplification Effects 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 238000003199 nucleic acid amplification method Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000011574 phosphorus Substances 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000228245 Aspergillus niger Species 0.000 description 2
- 241000235646 Cyberlindnera jadinii Species 0.000 description 2
- 244000168141 Geotrichum candidum Species 0.000 description 2
- 235000017388 Geotrichum candidum Nutrition 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 240000006024 Lactobacillus plantarum Species 0.000 description 2
- 235000013965 Lactobacillus plantarum Nutrition 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- LPQOADBMXVRBNX-UHFFFAOYSA-N ac1ldcw0 Chemical compound Cl.C1CN(C)CCN1C1=C(F)C=C2C(=O)C(C(O)=O)=CN3CCSC1=C32 LPQOADBMXVRBNX-UHFFFAOYSA-N 0.000 description 2
- 230000009418 agronomic effect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 229940072205 lactobacillus plantarum Drugs 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 235000021049 nutrient content Nutrition 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000012807 shake-flask culturing Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
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- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 description 1
- 241000157304 Prauserella rugosa Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
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- 241000235345 Schizosaccharomycetaceae Species 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 241000223261 Trichoderma viride Species 0.000 description 1
- 241000588902 Zymomonas mobilis Species 0.000 description 1
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- KLOIYEQEVSIOOO-UHFFFAOYSA-N carbocromen Chemical compound CC1=C(CCN(CC)CC)C(=O)OC2=CC(OCC(=O)OCC)=CC=C21 KLOIYEQEVSIOOO-UHFFFAOYSA-N 0.000 description 1
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- 230000036541 health Effects 0.000 description 1
- 125000004029 hydroxymethyl group Chemical group [H]OC([H])([H])* 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000010815 organic waste Substances 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
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Images
Abstract
The invention provides a kind of high-inorganic nitrogen-sourced biological compound fertilizer and preparation method thereof, it contains the following function bacterium: bacillus megaterium, Bacillus subtilus, yeast saccharomyces cerevisiae, plant lactobacillus, volume Mucor, streptomyces albus.The content of inorganic nitrogen in of composite fertilizer of the present invention can be up to 35%, and this moment, viable count still can reach 2 * 10
10Cfu/kg.Experiment shows that the field fertilizer efficiency of composite fertilizer of the present invention significantly is improved.
Description
Technical field
The present invention relates to a kind of composite fertilizer, the composite bio-fertilizer under specifically a kind of high nitrogenous source the invention still further relates to its preparation method.
Background technology
Fertilizer industry always is divided into three kinds of fertilizer, inorganic fertilizer and microorganism fertilizers, and in recent years for the concern to environment, agricultural-food health and pollution by chemical fertilizer, many producers begin to produce various composite fertilizers, and main products comprises two kinds of composite bio-fertilizer and compoiste fertilizers.Wherein biological organic fertilizer is to adopt microbiobacterial agent that organic waste is carried out the fermentative production fertilizer, and its biological activity is higher but available nutrient content is lower, and is subjected to the unsettled restriction of bio-fertilizer fertilizer efficiency; Compoiste fertilizer be after the organic castoff compost processing with chemical fertilizer mixing production composite fertilizer, be characterized in the equilibrium of organic-inorganic nutrient, available nutrient content height, but biological activity is lower, and its major cause is because be subjected to influence in high-inorganic nitrogen-sourced biological activity down.For addressing this problem, adopt some Inoculants during fertilizer and bio-fertilizer are produced at present, these Inoculants mainly are some single microbial inoculums, as vinelandii, P and K decomposing bacterium, photosynthetic bacterium etc., these bacterial classifications generally show action effect instability, weakness that fertilizer efficiency is lower.
CN1442394A (calling original application in the following text) discloses a kind of high nitrogen source fermentation method, it passes through screening VT microbial inoculum, and tames under high nitrogenous source, and therefrom screening obtains one group of function yeast, this group function yeast is sprayed onto high-inorganic nitrogen-sourced material surface, and this group function yeast comprises:
Plant lactobacillus (Lactobacillus plantarum) 10%, eight spore fission yeasts (Schizosaccharomyces octosporus) 15%, Candida utilis (Candida utilis) 20%, geotrichum candidum (Geotrichum candidum) 15%, have a liking for sour red Zymomonas mobilis (Rhodopeusdomaonas acidophila) 10%, aspergillus niger (Aspergillus niger) 10%, viride (Trichoderma viride) 10%, coarse promise Salmonella (Nocardia rugosa) 10%.
Through test in a few years, this method is still remaining to be improved aspect bacterial classification formation and the composite fertilizer's fertilizer efficiency, special this invention of proposition.
Summary of the invention
The object of the present invention is to provide a kind of composite bio-fertilizer preparation method under high-inorganic nitrogen-sourced, microorganism active and fertilizer efficiency are difficult to unified deficiency in the existing fertilizer product to overcome.
Another object of the present invention is to provide the method for the above-mentioned high nitrogenous source composite bio-fertilizer of preparation.
For achieving the above object, the present invention at first screens acquisition can keep active function yeast for one group in the presence of inorganic high nitrogenous source, this group function yeast can tolerate the urea more than 35%, specifically comprises: bacillus megaterium (Bacillus megaterium), yeast saccharomyces cerevisiae (Saccharomycescerevisiae), subtilis (Bacillus subtilis Cohn), plant lactobacillus (Lactobacillus plantarum), volume Mucor (Mucor abundans), streptomyces albus (Streptomyces albus).
The above-mentioned functions bacterium can be mixed with the microbial inoculum of following composition: bacillus megaterium 1.0 * 10
6～1.0 * 10
10Cfu/ml; Subtilis 1.0 * 10
6～1.0 * 10
10Cfu/ml; Yeast saccharomyces cerevisiae 1.0 * 10
3～1.0 * 10
8Cfu/ml; Plant lactobacillus 1.0 * 10
3～1.0 * 10
6Cfu/ml; Volume Mucor 1.0 * 10
2～1.0 * 10
4Cfu/ml; Streptomyces albus 1.0 * 10
3～1.0 * 10
6Cfu/ml.Preferred bacillus megaterium 1.0 * 10
8Cfu/ml, Bacillus subtilus 1.0 * 10
8Cfu/ml, yeast saccharomyces cerevisiae 1.0 * 10
4Cfu/ml, plant lactobacillus 1.0 * 10
4Cfu/ml, volume Mucor 1.0 * 10
3Cfu/ml, streptomyces albus 1.0 * 10
4Cfu/ml.
And then, the invention provides a kind of composite bio-fertilizer that contains the above-mentioned functions bacterium.The content of inorganic nitrogen in of this composite fertilizer can be up to 35%, and the total amount of the contained above-mentioned functions bacterium of every gram composite fertilizer can be 1 * 10
7~ 1 * 10
8Cfu is preferably 2 * 10
7Cfu.
The content of inorganic full nitrogen is preferably 5%～35% (amount by urea is calculated) in the composite fertilizer of the present invention.
The present invention also provides a kind of method for preparing inorganic high nitrogenous source composite bio-fertilizer, comprises microbial inoculum is mixed with the fertilizer that contains inorganic high nitrogenous source, preferably includes following steps:
1, makes above-mentioned each function yeast respectively by fermentation culture, and proportionally mix;
2, urea is carried out modification: the ratio in fertilizer (weight ratio) 5% ~ 35% is directly added urea in the organic materials abundant mixing, directly carry out expanded behind the mixing, its bulking pressure 2.1Mpa～3.0MPa, 95 ℃～120 ℃ of temperature of charge, 30 seconds～60 seconds time length.
3, material spraying: with carboxymethyl starch is bonding carrier, and the above-mentioned functions bacterium evenly is sprayed between expanded good material particles surface and the expanded slit, and the spraying inoculum size is with the total restatement 1%-4% of material.
Above-mentioned organic materials can be one or more in soya-bean cake, meat meal tankage, chicken manure, alfalfa meal, Semen Maydis powder, peat soil, furfural dregs, the pine needle powder etc., and through fermentative processing, the killing pathogenic bacteria worm's ovum is crossed 30 mesh sieves and obtained after the drying and crushing.
The content of inorganic nitrogen in of composite fertilizer of the present invention can be up to 35%, and this moment, viable count still can reach 2 * 10
10Cfu/kg.Experiment shows that the field fertilizer efficiency of composite fertilizer of the present invention significantly is improved.
Description of drawings
Fig. 1 is the flow process of each the function yeast single culture that the present invention relates to.
Embodiment
Following examples further specify content of the present invention, but should not be construed as limitation of the present invention.Without departing from the spirit and substance of the case in the present invention, modification or replacement to the inventive method, step or condition are done all belong to scope of the present invention.
If do not specialize the conventional means that used technique means is well known to those skilled in the art among the embodiment.
The preparation of embodiment 1 anti-inorganic high nitrogenous source microbial inoculum
1, anti-inorganic high nitrogenous source bacterium domestication
The substratum configuration:
The Gause I substratum:
KNO
3?1.0g
NaCL?0.5g
K
2HPO
4?0.5g
MgSO
47H
2O?0.5g
FeSO
4?0.01g
Zulkovsky starch 20g
Agar 20g
Water 1000ml
PH7.2～7.4
Beef-protein medium:
Extractum carnis 5g
Peptone 10g
NaCL??????5g
Agar 20g
Water 1000ml
PH7.4～7.6
Potato culture:
Potato 200g
Glucose 20g
Agar 20g
Water 1000ml
MRS substratum: casein peptone: 10g
Extractum carnis: 10g
Yeast extract paste: 5g
Glucose: 5g
Sodium acetate: 5g
Diammonium hydrogen citrate: 2g
MgSO4·7H
2O：?0.2g
MnSO4·H
2O：??0.05g
K
2HPO
4·H
2O：?2g
Tween80：?????1g
CaCO
3：???????20g
Agar 15g
Distilled water 1000ml
PH6.8
Do not add agar in above-mentioned each substratum, be liquid nutrient medium separately.
Acclimation method:
At first each function yeast is cultivated (bacillus megaterium, subtilis use Gause I substratum with the individual plant form on solid medium, streptomyces albus uses beef-protein medium, yeast saccharomyces cerevisiae uses potato culture, plant lactobacillus uses the MRS substratum, bacillus megaterium and subtilis are cultivated down at 37 ℃, other are in following 30 ℃ of cultivations), after waiting to produce born of the same parents internal bud cell type spore, collect thalline, join respectively in the corresponding liquid substratum, thalline is made 10
6～10
9Cfu/ml bacterium liquid, carry out shake-flask culture, then the different concns nitrogenous source is progressively added in the bacterium liquid, and carry out shake-flask culture, the concentration that adds nitrogenous source is progressively increased by lower concentration and (at first adds 5% urea, treat to add again after bacterium grows fine 10% urea, reach 35% until final urea content according to this method), be up to 35%.Three repetitions of each bacterial classification, the final naturalized strain that all obtains a plurality of anti-nitrogenous sources.
2, the function microbial inoculum is cultivated
Bacterial strain after the domestication is cultivated respectively as follows:
1) bacillus megaterium, subtilis
Bacterial classification is inoculated into seed culture medium by 5%, carries out the shake-flask seed fermentation culture, culture temperature is 37 ℃, cultivates 36 hours; By inoculum size 5% inoculation fermentation substratum, in fermentor tank, carry out fermentation culture, culture condition: 37 ℃, 200 rev/mins, cultivate and promptly got function yeast in 72 hours.
2) streptomyces albus
Bacterial classification is inoculated into seed culture medium by 5%, carries out the shake-flask seed fermentation culture, culture temperature is 30 ℃, cultivates 96 hours; By inoculum size 5% inoculation fermentation substratum, in fermentor tank, carry out fermentation culture, culture condition: 30 ℃, 150 rev/mins, cultivate and promptly got function yeast in 168 hours.
3) yeast saccharomyces cerevisiae
Bacterial classification is inoculated into the PDA substratum by 5%, carries out the shake-flask seed fermentation culture, culture temperature is 30 ℃, cultivates 36 hours; By inoculum size 5% inoculation fermentation substratum, in fermentor tank, carry out fermentation culture, culture condition: 30 ℃, 150 rev/mins, cultivate and promptly got function yeast in 48 hours.
4) plant lactobacillus
Bacterial classification is inoculated into the MRS substratum by 5%, carries out the shake-flask seed fermentation culture, culture temperature is 30 ℃, cultivates 48 hours; By inoculum size 5% inoculation fermentation substratum, in fermentor tank, carry out fermentation culture, culture condition: 30 ℃, leave standstill cultivation, cultivate and promptly got function yeast in 48 hours.
5) volume Mucor
Bacterial classification is inoculated into seed culture medium by 5%, carries out the shake-flask seed fermentation culture, culture temperature is 30 ℃, cultivates 36 hours; By inoculum size 5% inoculation fermentation substratum, in fermentor tank, carry out fermentation culture, culture condition: 30 ℃, 150 rev/mins, cultivate and promptly got function yeast in 168 hours.
Above-mentioned fermention medium is identical with seed culture medium.
3, the microorganism collection that step 2 is obtained is to phosphoric acid buffer (0.1M concentration pH6.8, contain 1% solubility monose) in (microorganism collection uses modes such as centrifugal settling, filtration, absorption, to collect thalline then and claim wet bacterium weight, use phosphoric acid buffer dilution 5-10 doubly), measure viable count 10
3-10
10Cfu/ml, method is used microscopic count method or colony counting method.
4, preparation microbial inoculum spray coating liquor
Microorganism collection is contained in the 1% solubility monose to 0.1M pH6.8 phosphoric acid buffer, following ratio (v/v): bacillus megaterium 20%, subtilis 20%, streptomyces albus 20%, yeast saccharomyces cerevisiae 15%, plant lactobacillus 15%, volume Mucor 10%, mix the back and add 2% (w/w) hydroxymethyl starch, make the microbial inoculum spray coating liquor.
Prepare the spray coating liquor of following different size as stated above
Spray coating liquor constitutes 1
Bacillus megaterium 1.0 * 10
8Cfu/ml
Bacillus subtilus 1.0 * 10
8Cfu/ml
Yeast saccharomyces cerevisiae 1.0 * 10
4Cfu/ml
Plant lactobacillus 1.0 * 10
4Cfu/ml
Volume Mucor 1.0 * 10
3Cfu/ml
Streptomyces albus 1.0 * 10
4Cfu/ml
Spray coating liquor constitutes 2
Bacillus megaterium 1.0 * 10
10Cfu/ml
Bacillus subtilus 5.0 * 10
7Cfu/ml
Yeast saccharomyces cerevisiae 1.8 * 10
6Cfu/ml
Plant lactobacillus 1.0 * 10
6Cfu/ml
Volume Mucor 1.0 * 10
4Cfu/ml
Streptomyces albus 1.0 * 10
3Cfu/ml
Spray coating liquor constitutes 3
Bacillus megaterium 1.0 * 10
6Cfu/ml
Bacillus subtilus 1.0 * 10
10Cfu/ml
Yeast saccharomyces cerevisiae 1.0 * 10
3Cfu/ml
Plant lactobacillus 1.0 * 10
3Cfu/ml
Volume Mucor 1.0 * 10
4Cfu/ml
Streptomyces albus 1.0 * 10
6Cfu/ml
Spray coating liquor constitutes 4
Bacillus megaterium 5.0 * 10
9Cfu/ml
Bacillus subtilus 1.0 * 10
6Cfu/ml
Yeast saccharomyces cerevisiae 5.0 * 10
4Cfu/ml
Plant lactobacillus 5.5 * 10
3Cfu/ml
Volume Mucor 1.5 * 10
3Cfu/ml
Streptomyces albus 2.0 * 10
5Cfu/ml
The preparation of the high nitrogenous source of embodiment 2 composite fertilizer
1) fertilizer preparation: purchase commercially available compound and dash fertilising: nitrogenous (N) 10%, phosphorus (P
2O
5) 4%, potassium (K
2O) 6%, packing specifications 50Kg/ bag.Component is urea, potassium primary phosphate, Repone K, and indicating total nutrient is 20% (nitrogen, phosphorus, potassium amount to).The microbial inoculum for preparing in proportion sprays to the compound fertilizer granule surface with 8% (weight ratio), treat the fertilizer drying after, the pack, promptly get high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer.
2) manure trial
Test site: Hainan Province decides the Dingan County cities and towns and carries out, and experimental field soil property is a sandy loam, and soil fertility is medium, and preceding crop is a cucumber.
Test kind: the bluish white dish in Shanghai
For trying fertilizer: " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " contains effective viable count 〉=0.2 hundred million/gram, N+P
2O
5+ K
2O=20
Test design: 3 processing are established in test altogether, and each is handled 3 times and repeats random alignment, sub-district area 20M
2Treatment Design is as follows:
Handle one: use high nitrogenous source bio-feritlizer
Handle two: the composite fertilizer of using deactivation
Handle three: blank (not executing any fertilizer)
The cultivation management situation: Chinese cabbage was sowed on December 15th, 2006, thinning on January 13 in 2007, and mu is planted 8.8 ten thousand strains, and February 13 gathered in the crops.Handle one mu and execute " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " 100Kg and make base manure and once apply, handle two mu of " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " 100Kg that execute deactivation and make base manure and once apply, handle three and do not execute any fertilizer.
Result and analysis: use " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " influence to the Chinese cabbage Other Main Agronomic Characters.
As can be seen from Table 1, the plant height of handling a Chinese cabbage is respectively than handling two, handling three increase by 12.8% and 24.7%, and blade length increases by 13.6% and 20.7% respectively, and width increases 9.6% and 32.1% respectively; Handling two plant heights increases by 10.6% than handling three, and blade length increases by 6.2%, and width increases 20.5%.Explanation is compared with the blank that does not apply fertilizer, " the high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " that use " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " and deactivation all can promote the Chinese cabbage growth, increase Chinese cabbage plant height, blade length and width, the increasing degree of using " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " is greater than " the high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " that use deactivation
Table 1 is used " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " influence to the Chinese cabbage Other Main Agronomic Characters
Use " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " influence to Chinese cabbage output
As can be seen from Table 2, the Chinese cabbage poor heteropole between processing one and processing two, the processing three is remarkable.It is the highest to handle a Chinese cabbage output, handles two and takes second place, and handles three minimum.
Use 58.4 kilograms of the processing one Chinese cabbage sub-district mean yields of " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer ", 1946.7 kilograms of folding per mu yields increase 183.4 kilograms than handling two mu, and amplification 10.4% is increased production extremely remarkable; Increase 836.7 kilograms than handling three, amplification 75.4% is increased production extremely remarkable.Use 52.9 kilograms of the processing two Chinese cabbage sub-district mean yields of " the high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " of deactivation, 1763.3 kilograms of folding per mu yields increase 653.3 kilograms than handling three mu, and amplification 58.8% is increased production extremely remarkable.As seen compare with the blank that does not apply fertilizer, " the high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " that use " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " and deactivation all can improve Chinese cabbage output, and the ratio of using " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " is used " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " increase yield significantly of deactivation.
Table 2 is used " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " influence to Chinese cabbage output
Annotate: the different lowercases in data back are represented to check significant difference through LSR in the table; Different capitalizations are represented through LSR check difference extremely remarkable.
Conclusion
Use " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " and deactivation " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " and all can promote the Chinese cabbage growth, increase Chinese cabbage plant height, blade length and width, it is bigger than " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " increasing degree of using deactivation to use " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer ".
Chinese Cabbage than using deactivation " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " volume increase 10.4%, is increased production extremely remarkable with " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer "; Than the blank volume increase 75.4% of not applying fertilizer, increase production extremely remarkable.Than the blank volume increase 58.8% of not applying fertilizer, effect of increasing production is extremely remarkable with deactivation " high-inorganic nitrogen-sourced biological microbial inoculum composite fertilizer " for Chinese Cabbage.
The preparation of the high nitrogenous source of embodiment 3 composite fertilizer
1), organic materials preparation
Batching: dried poultrymanure 60% (weight ratio), alditol slag 30%, dregs of beans 10%
Above-mentioned material is ground into 5mm left and right sides particle, inserts 1-2 ‰ (weight ratio) microbial inoculum of the present invention, moisture is transferred to about 60%, piles the 0.8-1m height, wide 0.5-1m, and solid fermentation 7-10 days, fermentation ends, drying and crushing is crossed 30 mesh sieves, obtains organic materials.
2), mixture puffing
Urea and organic materials are mixed according to weight ratio 15%, and directly expanded, bulking pressure is 2.3Mpa, and 105 ℃ of temperature of charge continue 35 seconds.
3), microbial inoculum spraying
With the carboxymethyl starch aqueous solution microbial inoculum of the present invention is modulated into and contains viable count about 10
9Cfu/ml liquid uses atomizer directly to spray, and spraying bacterium liquid measure is a weight of material 6%, treat that moisture is qualified after, the packing warehouse-in.
Microbial inoculum of the present invention is optimized through the bacterial classification adjustment, and the microbial inoculum contrast with in the original application microbial inoculum has (1) and utilizes high nitrogenous source ability; (2) produce born of the same parents' Endospore, viability height; (3) have phosphorus decomposing, potassium decomposing function (seeing Table 3).
Microbial inoculum among table 3 the present invention and original application microbial inoculum are relatively
| Microbial inoculum | Effective bacterium number (cfu/ml) | The thalline Endospore forms per-cent (%) | Validity period (moon) under the 15% high nitrogenous source condition |
| The microbial inoculum of implementing | ??10 6-10 10 | ??40-60 | ??18 |
| The original application microbial inoculum | ??10 5-10 8 | ??15-40 | ??9 |
The composite bio-fertilizer for preparing 35% inorganic high nitrogenous source as stated above, initial total viable count is about 3.0 * 10
10Cfu/kg, normal temperature placed after 2 months, detected viable count still up to 2.6 * 10
10Cfu/kg.
Claims (9)
1, a kind of microbial inoculum, it contains the following function yeast of anti-35% above urea after taming: bacillus megaterium 1.0 * 10
6～1.0 * 10
10Cfu/ml, Bacillus subtilus 1.0 * 10
6～1.0 * 10
10Cfu/ml, yeast saccharomyces cerevisiae 1.0 * 10
3～1.0 * 10
8Cfu/ml, plant lactobacillus 1.0 * 10
3～1.0 * 10
6Cfu/ml, volume Mucor 1.0 * 10
2～1.0 * 10
4Cfu/ml and streptomyces albus 1.0 * 10
3～1.0 * 10
6Cfu/ml.
2, microbial inoculum as claimed in claim 1, it contains following function yeast: bacillus megaterium 1.0 * 10
8Cfu/ml, Bacillus subtilus 1.0 * 10
8Cfu/ml, yeast saccharomyces cerevisiae 1.0 * 10
4Cfu/ml, plant lactobacillus 1.0 * 10
4Cfu/ml, volume Mucor 1.0 * 10
3Cfu/ml and streptomyces albus 10
4Cfu/ml.
3, claim 1 or the 2 described function yeast application in the inorganic high nitrogenous source composite bio-fertilizer of preparation.
4, a kind of high-inorganic nitrogen-sourced biological compound fertilizer, it contains claim 1 or 2 described microbial inoculums.
5, composite fertilizer as claimed in claim 4, its inorganic nitrogen-sourced content is 5%～35%.
6, as claim 4 or 5 described composite fertilizers, it is characterized in that the viable count 1.0 * 10 in the composite fertilizer
7～1.0 * 10
11Cfu/kg.
7, composite fertilizer as claimed in claim 6 is characterized in that, the viable count 2.0 * 10 in the composite fertilizer
10Cfu/kg.
8, a kind of method for preparing each described composite fertilizer of claim 4～7, it comprises claim 1 or 2 described microbial inoculums is mixed with the fertilizer that contains inorganic high nitrogenous source.
9, method as claimed in claim 8, it comprises the steps:
1). make described each function yeast of claim 1 respectively by independent fermentation culture, and proportionally mix, get microbial inoculum;
2). urea is carried out modification: the ratio that accounts for total amount 5%～35% in the urea addition is directly added urea in the organic materials abundant mixing, directly carry out expanded behind the mixing, its bulking pressure 2.1 Mpa～3.0 MPa, 95 ℃～120 ℃ of temperature of charge, 30 seconds～60 seconds time length;
3). the material spraying: with carboxymethyl starch is bonding carrier, and the microbial inoculum that step 1) is made evenly is sprayed between expanded good material particles surface and the expanded slit, and the spraying inoculum size is with the total restatement 1%～4% of material.
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