CN104839228A - Crop yield increasing agent and preparation process thereof - Google Patents
Crop yield increasing agent and preparation process thereof Download PDFInfo
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- CN104839228A CN104839228A CN201510181852.XA CN201510181852A CN104839228A CN 104839228 A CN104839228 A CN 104839228A CN 201510181852 A CN201510181852 A CN 201510181852A CN 104839228 A CN104839228 A CN 104839228A
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- bacillus subtilis
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Abstract
The invention relates to a crop yield increasing agent and a preparation process thereof. In the prior art, the existing crop yield increasing agent has disadvantages of heavy environmental pollution, easy residue generation on crops, and harm to health. A purpose of the present invention is to solve the problems in the prior art. The yield increasing agent is prepared from Candida, Bacillus subtilis and a carrier. The preparation process comprises: 1, carrying out propagation on Candida and Bacillus subtilis; 2, inoculating the obtained Candida into a malt wort culture medium, inoculating the obtained Bacillus subtilis into a nutrient broth culture medium, and culturing to obtain a Candida liquid and a Bacillus subtilis liquid; 3, adding the Candida liquid to a carrier, uniformly stirring, fermenting, drying, adding the Bacillus subtilis liquid, fermenting, and drying to obtain bacterial powder; and 4, adding the bacterial powder to a NaOH aqueous solution, stirring, carrying out centrifugation, taking the supernatant, and adjusting the pH value so as to obtain the crop yield increasing agent. The crop yield increasing agent of the present invention is used for crop yield increasing.
Description
Technical field
The present invention relates to a kind of increasing crop yield agent and preparation technology thereof.
Background technology
Increasing agent refers to improve to crop yield the useful nutritional agents or the conditioning agent that have facilitation, is preparation agriculturally indispensable at present.But existing increasing crop yield agent mostly is chemical composition, environmental pollution is comparatively serious, and easily remains on crop, brings hidden danger to health of people.
Summary of the invention
The present invention will solve existing increasing crop yield agent environmental pollution comparatively seriously, and easily remains on crop, the problem of insalubrity, provides a kind of increasing crop yield agent and preparation technology thereof.
Increasing crop yield agent of the present invention is made up of candidiasis, bacillus subtilis and carrier, viable bacteria content>=5 × 10 of described candidiasis
7cfu/mL, viable bacteria content>=1 × 10 of described bacillus subtilis
9cfu/mL, described carrier by weight percentage by the corn flour of 80%, the dregs of beans of 10% and 10% wheat bran form, the weight of candidiasis accounts for 40% of total weight of carrier, and the weight of bacillus subtilis accounts for 40% of total weight of carrier.
Described candidiasis is bought from Institute of Microbiology, Heilongjiang Academy of Sciences, is candida utili (Candida utilis), is numbered 2.1180.
Described bacillus subtilis is bought from Institute of Microbiology, Heilongjiang Academy of Sciences, is bacillus subtilis (Bacillus Subtilis), is numbered 1.421.
The preparation technology of above-mentioned increasing crop yield agent, carries out according to the following steps:
One, candidiasis is inoculated on wort agar medium, cultivates 7 days at 27 DEG C, obtain expanding numerous candidiasis, bacillus subtilis is inoculated on nutrient broth medium, cultivate 6 days at 27 DEG C, obtain expanding numerous bacillus subtilis;
Two, be inoculated in malt extract medium by expanding the inoculum concentration of numerous candidiasis according to 2%, be inoculated in nutrient broth medium by expanding the inoculum concentration of numerous bacillus subtilis according to 2%, in 27 DEG C of shaking table shaken cultivation 4 days, shaking speed is 90 ~ 100 turns/min, obtains candidiasis bacterium liquid and bacillus subtilis bacterium liquid;
Three, take by weight percentage the corn flour of 80%, the dregs of beans of 10% and 10% wheat bran mix, obtain carrier, in carrier, add candidiasis bacterium liquid stir, in 27 DEG C of fermentations 4 days, then 70 ~ 90 DEG C of oven dry, to water content lower than 10%, add bacillus subtilis bacterium liquid again to stir, in 27 DEG C of fermentations 4 days, then 90 ~ 100 DEG C of oven dry, to water content lower than 12%, obtain bacterium powder;
Four, bacterium powder being joined mass concentration is stir 50 minutes in the NaOH aqueous solution of 1%, then adjust ph to 7, leaves standstill centrifugal after 3 days, is get supernatant, then regulates the pH value to 3 of supernatant, namely obtain increasing crop yield agent after centrifugal.
Using method of the present invention: add water and mix in increasing crop yield agent during use, is then sprayed on blade face, at Crop development midterm spray.The volume ratio of increasing crop yield agent and water is 1:100.
Beneficial effect of the present invention:
1, increasing agent of the present invention is biologic product, without any chemical composition, pollution-free, noresidue;
2, increasing agent of the present invention can make corn yield increasing 15% ~ 20%, increasing production of rice 10% ~ 15%, soybean yield-increasing 10% ~ 15%, vegetable increase production more than 20%;
3, increasing agent of the present invention can urge precocious 7 ~ 10 days, full seed, and flat grain obviously reduces, and improves crop product quality, increases economic efficiency.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises any combination between each embodiment.
Embodiment one: the agent of present embodiment increasing crop yield is made up of candidiasis, bacillus subtilis and carrier, viable bacteria content>=5 × 10 of described candidiasis
7cfu/mL, viable bacteria content>=1 × 10 of described bacillus subtilis
9cfu/mL, described carrier by weight percentage by the corn flour of 80%, the dregs of beans of 10% and 10% wheat bran form, the weight of candidiasis accounts for 40% of total weight of carrier, and the weight of bacillus subtilis accounts for 40% of total weight of carrier.
Embodiment two: the preparation technology of present embodiment increasing crop yield agent, carry out according to the following steps:
One, candidiasis is inoculated on wort agar medium, cultivates 7 days at 27 DEG C, obtain expanding numerous candidiasis, bacillus subtilis is inoculated on nutrient broth medium, cultivate 6 days at 27 DEG C, obtain expanding numerous bacillus subtilis;
Two, be inoculated in malt extract medium by expanding the inoculum concentration of numerous candidiasis according to 2%, be inoculated in nutrient broth medium by expanding the inoculum concentration of numerous bacillus subtilis according to 2%, in 27 DEG C of shaking table shaken cultivation 4 days, shaking speed is 90 ~ 100 turns/min, obtains candidiasis bacterium liquid and bacillus subtilis bacterium liquid;
Three, take by weight percentage the corn flour of 80%, the dregs of beans of 10% and 10% wheat bran mix, obtain carrier, in carrier, add candidiasis bacterium liquid stir, in 27 DEG C of fermentations 4 days, then 70 ~ 90 DEG C of oven dry, to water content lower than 10%, add bacillus subtilis bacterium liquid again to stir, in 27 DEG C of fermentations 4 days, then 90 ~ 100 DEG C of oven dry, to water content lower than 12%, obtain bacterium powder;
Four, bacterium powder being joined mass concentration is stir 50 minutes in the NaOH aqueous solution of 1%, then adjust ph to 7, leaves standstill centrifugal after 3 days, is get supernatant, then regulates the pH value to 3 of supernatant, namely obtain increasing crop yield agent after centrifugal.
Embodiment three: present embodiment and embodiment two unlike: the wort agar medium in step one and nutrient broth medium are solid culture medium, and the malt extract medium in step 2 and nutrient broth medium are liquid nutrient medium.Other is identical with embodiment two.
Embodiment four: present embodiment and embodiment two or three are unlike viable bacteria content>=5 × 10 of the candidiasis bacterium liquid that step 2 obtains
7cfu/mL.Other is identical with embodiment two or three.
Embodiment five: one of present embodiment and embodiment two to four are unlike viable bacteria content>=1 × 10 of the bacillus subtilis bacterium liquid that step 2 obtains
9cfu/mL.Other is identical with one of embodiment two to four.
Embodiment six: one of present embodiment and embodiment two to five unlike: in step 3, the weight of candidiasis bacterium liquid accounts for 40% of vehicle weight, and the weight of bacillus subtilis bacterium liquid accounts for 40% of vehicle weight.Other is identical with one of embodiment two to five.
For verifying beneficial effect of the present invention, carry out following experiment:
Embodiment:
The preparation technology of the present embodiment increasing crop yield agent, carries out according to the following steps:
One, candidiasis is inoculated on wort agar medium, cultivates 7 days at 27 DEG C, obtain expanding numerous candidiasis, bacillus subtilis is inoculated on nutrient broth medium, cultivate 6 days at 27 DEG C, obtain expanding numerous bacillus subtilis;
Two, be inoculated in malt extract medium by expanding the inoculum concentration of numerous candidiasis according to 2%, be inoculated in nutrient broth medium by expanding the inoculum concentration of numerous bacillus subtilis according to 2%, in 27 DEG C of shaking table shaken cultivation 4 days, shaking speed is 100 turns/min, obtains candidiasis bacterium liquid and bacillus subtilis bacterium liquid;
Three, take by weight percentage the corn flour of 80%, the dregs of beans of 10% and 10% wheat bran mix, obtain carrier, in carrier, add candidiasis bacterium liquid stir, in 27 DEG C of fermentations 4 days, then 80 DEG C of oven dry, to water content lower than 10%, add bacillus subtilis bacterium liquid again to stir, in 27 DEG C of fermentations 4 days, then 90 DEG C of oven dry, to water content lower than 12%, obtain bacterium powder;
Four, bacterium powder being joined mass concentration is stir 50 minutes in the NaOH aqueous solution of 1%, then adjust ph to 7, leaves standstill centrifugal after 3 days, is get supernatant, then regulates the pH value to 3 of supernatant, namely obtain increasing crop yield agent after centrifugal.
Described candidiasis is bought from Institute of Microbiology, Heilongjiang Academy of Sciences, is candida utili (Candida utilis), is numbered 2.1180.
Described bacillus subtilis is bought from Institute of Microbiology, Heilongjiang Academy of Sciences, is bacillus subtilis (Bacillus Subtilis), is numbered 1.421.
Plant for paddy rice, corn, soybean and vegetables, cultivated area, for being 10 mu, arranges experimental group and control group, the increasing crop yield agent that experimental group is prepared at Crop development midterm spray the present embodiment, and control group sprays certain commercially available brand increasing agent.The using method of the present embodiment increasing crop yield agent: add water and mix in increasing crop yield agent, is then sprayed on blade face, at Crop development midterm spray.The volume ratio of increasing crop yield agent and water is 1:100.Experimental result is as shown in the table.
The plantation result of each crop of table 1 experimental group
The plantation result of each crop of table 2 control group
From the data in table 1 and table 2, the obvious effect of increasing production of increasing agent of the present invention, can make corn yield increasing 15% ~ 20%, increasing production of rice 10% ~ 15%, soybean yield-increasing 10% ~ 15%, vegetable increase production more than 20%; Increasing agent of the present invention can urge precocious 7 ~ 10 days, full seed, and flat grain obviously reduces, and improves crop product quality, increases economic efficiency.
Claims (6)
1. increasing crop yield agent, is characterized in that this increasing agent is made up of candidiasis, bacillus subtilis and carrier, viable bacteria content>=5 × 10 of described candidiasis
7cfu/mL, viable bacteria content>=1 × 10 of described bacillus subtilis
9cfu/mL, described carrier by weight percentage by the corn flour of 80%, the dregs of beans of 10% and 10% wheat bran form, the weight of candidiasis accounts for 40% of total weight of carrier, and the weight of bacillus subtilis accounts for 40% of total weight of carrier.
2. the preparation technology of increasing crop yield agent as claimed in claim 1, is characterized in that this technique is carried out according to the following steps:
One, candidiasis is inoculated on wort agar medium, cultivates 7 days at 27 DEG C, obtain expanding numerous candidiasis, bacillus subtilis is inoculated on nutrient broth medium, cultivate 6 days at 27 DEG C, obtain expanding numerous bacillus subtilis;
Two, be inoculated in malt extract medium by expanding the inoculum concentration of numerous candidiasis according to 2%, be inoculated in nutrient broth medium by expanding the inoculum concentration of numerous bacillus subtilis according to 2%, in 27 DEG C of shaking table shaken cultivation 4 days, shaking speed is 90 ~ 100 turns/min, obtains candidiasis bacterium liquid and bacillus subtilis bacterium liquid;
Three, take by weight percentage the corn flour of 80%, the dregs of beans of 10% and 10% wheat bran mix, obtain carrier, in carrier, add candidiasis bacterium liquid stir, in 27 DEG C of fermentations 4 days, then 70 ~ 90 DEG C of oven dry, to water content lower than 10%, add bacillus subtilis bacterium liquid again to stir, in 27 DEG C of fermentations 4 days, then 90 ~ 100 DEG C of oven dry, to water content lower than 12%, obtain bacterium powder;
Four, bacterium powder being joined mass concentration is stir 50 minutes in the NaOH aqueous solution of 1%, then adjust ph to 7, leaves standstill centrifugal after 3 days, is get supernatant, then regulates the pH value to 3 of supernatant, namely obtain increasing crop yield agent after centrifugal.
3. the preparation technology of increasing crop yield agent according to claim 2, it is characterized in that wort agar medium in step one and nutrient broth medium are solid culture medium, the malt extract medium in step 2 and nutrient broth medium are liquid nutrient medium.
4. the preparation technology of the increasing crop yield agent according to Claims 2 or 3, is characterized in that viable bacteria content>=5 × 10 of the candidiasis bacterium liquid that step 2 obtains
7cfu/mL.
5. the preparation technology of increasing crop yield agent according to claim 4, is characterized in that viable bacteria content>=1 × 10 of the bacillus subtilis bacterium liquid that step 2 obtains
9cfu/mL.
6. the preparation technology of increasing crop yield agent according to claim 5, it is characterized in that the weight of candidiasis bacterium liquid in step 3 accounts for 40% of vehicle weight, the weight of bacillus subtilis bacterium liquid accounts for 40% of vehicle weight.
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| CN101914447A (en) * | 2010-07-31 | 2010-12-15 | 大连三科生物工程有限公司 | Complex microbial inoculum 707 and preparation method and application thereof |
| CN103483020A (en) * | 2013-09-13 | 2014-01-01 | 成都天星农业科技有限公司 | Bio-organic fertilizer and method for preparing bio-organic fertilizer which prevents and treats soil insects |
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2015
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Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1618772A (en) * | 2003-11-20 | 2005-05-25 | 郭伟光 | High efficiency biological organic fertilizer and its production technology |
| CN101144098A (en) * | 2007-07-18 | 2008-03-19 | 武汉烁森生物科技有限公司 | Preparation method of nitrogen source-plant peptide for fermentation and application thereof |
| CN101235293A (en) * | 2008-03-03 | 2008-08-06 | 上海创博生态工程有限公司 | Composite microorganism preparation for accelerating root system growing and reducing disease and preparing method thereof |
| CN101619006A (en) * | 2009-05-17 | 2010-01-06 | 冯子龙 | Novel biological nitrogen fertilizer and fermentation technology thereof |
| CN101603019A (en) * | 2009-07-23 | 2009-12-16 | 北京沃土天地生物科技有限公司 | A kind of high-inorganic nitrogen-sourced biological compound fertilizer and preparation method thereof |
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| CN101830737A (en) * | 2010-04-09 | 2010-09-15 | 华南农业大学 | Chemical-biological straw decomposing inoculant and decomposing method thereof |
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Application publication date: 20150819 |