CN101235293A - Composite microorganism preparation for accelerating root system growing and reducing disease and preparing method thereof - Google Patents
Composite microorganism preparation for accelerating root system growing and reducing disease and preparing method thereof Download PDFInfo
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- CN101235293A CN101235293A CNA200810034166XA CN200810034166A CN101235293A CN 101235293 A CN101235293 A CN 101235293A CN A200810034166X A CNA200810034166X A CN A200810034166XA CN 200810034166 A CN200810034166 A CN 200810034166A CN 101235293 A CN101235293 A CN 101235293A
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Abstract
The invention relates to composite agent which is used to accelerate the growth of root of crop and the growth of plant, and is characterized in that bacillus licheniformis, brewers' yeast, rhizopus and streptomycete pass through the fermentation technology which is individually cultivated by liquid and is expansively cultivated by solid through adopting product augmentor and viable bacteria carrier, which can make the total beneficial viable bacteria reach 100 billion/g through and keep all active metabolite. After applied in soil, the agent can promote the growth of crop root, increases crop to absorb phosphorous and potassium, degrading toxic substances in soil, increases stress resistance of corps and promotes the formation of humus, which is a green soil modifying product which is safe, high efficiency and non residual.
Description
Technical field
The present invention relates to a kind of complex microorganism preparations and preparation method who promotes root system growing and reducing disease, this complex microorganism preparations can be used by root system of plant in soil, can promote plant root system development, reduce the root diseases generation and the environment of improving the soil, belong to the soil improvement technical field.
Background technology
Soil is the base camp of nature microorganism, it has possessed various microorganism growth and has grown conditions such as needed nutrient water air potential of hydrogen osmotic pressure and temperature, so soil is given the good environment of microorganism life, great majority can obtain by different selection substratum from soil in the existing microorganism, but, soil microorganisms is subjected to envrionment conditions as rainfall, soil property, temperature, the influence of rate of permeation, often can't provide plant growth required microorganism, carry out the conversion of matter and energies such as nitrogen phosphorus potassium, root system is that plant carries out the nutrition decomposition, utilize and be transported to the primary structure of each vegetative organ, its grow and on every side the microorganism growth environment directly have influence on the absorption of nutritive substance and grow, rhizosphere is a very special film micro area, it is because the influence of root system of plant, make around it little territory physical chemistry with biological aspect different with the soil main body, the organic substance from root system in the rhizosphere soil can account for about 30% of plant photosynthesis product; Its composition can be from simple carbohydrate amino acid to complexity growth substance etc., so rhizospheric microorganism is under normal operation than non-rhizosphere showed increased.
In recent years, for output is provided, plough and use chemical fertilizer in a large number, for reducing insect pest, crop is sprayed insecticide in a large number, chemical fertilizer makes potential of hydrogen acute variation in the soil solution, objectionable impurities has directly also been murdered most of beneficial microorganisms in the agricultural chemicals when killing and wounding insect, and these complicated industrial compounds can be preserved century-old in soil, and can't be by microbiological degradation, so destruction of the going to wreck property of microbial environment of soil root system, show: fertility descends year by year, and it is serious day by day to harden, and crop can't carry out continuous cropping, simultaneously using of agricultural chemicals makes that hazard residue increases year by year in the crop, and serious has caused some to threaten the disease of human healths.How recovering the normal microbial environment of soil, guarantee that root system carries out the conversion of matter and energies such as nitrogen phosphorus potassium, is the subject matter of current soil environmental modification technuiques.
In the time of applying pesticides or weedicide, the microorganism in the soil all is killed, and causes the decomposition of soil organic matter to be interrupted, and accounts for most spoilage organism at occurring in nature thereafter and also occupy advantage easily in soil.The pathogenic bacteria or the insect that have certain resistance are simultaneously just constantly evolved in this multiple environment, and residual toxicity is strong more, just may promote the resistant gene reorganization of pathogenic bacteria or insect more.
At present, a disclosed International PCT patent, " plant growth promoter and method of promoting plant growth " by name, notification number is CN 1449251A, and it is the microorganism that comprises the microorganism of the microbial plant growth promoter of the microorganism of Staphylococcus and brevibacterium sp and Staphylococcus and brevibacterium sp is added in the soil and to promote the method for plant-growth.Even can make plant be unsuitable on the bad fertility bed soil of growth and development of plants, also can be steady in a long-term grow, it is simply to be mixed by the bacterial classification that two bacterioids belong to, there are not other carriers, can't in different edatopes, normally survive, simultaneously also can't produce antibiotic and resist the erosion of disease,, be difficult to play the net effect that promotes growth and defence disease so be a kind of primary growth stimulant to root system of plant.
Summary of the invention
The purpose of this invention is to provide a kind of micro-ecological environment that can effectively improve crop root, promote the beneficial microorganism growth and breeding, suppress simultaneously and kill and wound to be harmful to humic bacterium or disease and pest, promote the normal development of root system, thereby promote plant growth to reduce or stop applying pesticides, reach a kind of complex microorganism preparations and preparation method of safe, green planting effect.
For realizing above purpose, technical scheme of the present invention provides a kind of complex microorganism preparations that promotes root system growing and reducing disease, it is characterized in that, is made up of following weight part raw material: 40 parts-60 parts of microbial strain culture, 60 parts-40 parts in beneficial microorganism carrier.
The bacterial classification of described microbial strain culture is made up of following weight part raw material: 10 parts-15 parts of Bacillus licheniformis, 6 parts-8 parts of saccharomyces cerevisiaes, 3 parts-5 parts of rhizopus, 2 parts-3 parts of streptomycetes.
Described beneficial microorganism carrier is zeolite, bentonitic a kind of or its mixture.
A kind of preparation method who promotes the complex microorganism preparations of root system growing and reducing disease is characterized in that, making processes of the present invention is:
The first step. take by weighing bacterial classification: take by weighing each bacterial classification according to following weight part: 10 parts-15 parts of Bacillus licheniformis,
6 parts-8 parts of saccharomyces cerevisiaes, 3 parts-5 parts of rhizopus, 2 parts-3 parts of streptomycetes;
Second step. the liquid culture of Bacillus licheniformis, saccharomyces cerevisiae
A. mixed strains: the Bacillus licheniformis and the saccharomyces cerevisiae that take by weighing in the step 1 are mixed;
B. liquid culture: liquid nutrient medium 120 ℃-130 ℃, through 30 minutes-45 minutes high-temperature sterilizations, in substratum, add the tween of 0.3%-0.5%, with mixed bacterial classification in 1: the ratio of 25-30 is inoculated in the liquid nutrient medium, in fermentor tank, stirred 20 minutes, its rotating speed is 220-240 rev/min, put into sterilization back plastic tank after stirring and left standstill 5-7 days, leave standstill the gas of measuring Ph value and release output in the process every day; Sampling is 3 times in the fermenting process, and microscopically detects the thalli growth situation and measures Ph value, stops to ferment when 1,000,000,000/ml concentration and Ph value reach 3.0-4.0 when total viable count reaches;
The 3rd step. the solid enlarged culturing of bacterial classification
A. with the bacterium liquid of the compound cultivation of step 2 in 1: the ratio of 25-30 inserts in the solid enlarged culturing base of 120 ℃ of-130 ℃, 30 minutes-45 minutes high-temperature sterilizations, add rhizopus in the step 1, streptomycete simultaneously, stir 20 minutes-30 minutes to even, pour sealing and fermenting in the double-deck feedbag of sterilization into, temperature is 30 ℃-35 ℃, 7 days-9 days time;
B. take a sample three times in the fermenting process, measure moisture and Ph value, take a sample after the fermentation ends, mensuration moisture is that 35%-40%, Ph value are 4.5-6.0; Total viable count 1,000,000,000/g secondary fermentation finishes;
The 4th step. the dry carrier that adds
A. dry: the product of step 3 fermentation finished thoroughly1 is in chronological sequence put into the incubator drying, and temperature is controlled at 42 ℃-45 ℃, 10 hours-12 hours time, and the material moisture content that drying is finished is controlled at≤and 25%;
B. add carrier: dried microbial strain culture is added carrier, its weight part is: microbial strain culture 40-60%, carrier 60-40%, pour in the stirrer and stir, changing pulverizer then over to pulverizes, temperature of charge is controlled at below 45 ℃, material after the pulverizing is crossed 50 eye mesh screens, and sieve back coarse fodder is pulverized again;
The 5th step. packing
Product after sieving is incorporated with in the packing bag of individual event purging valve, preserves at dry, lucifuge place.
Liquid nutrient medium in the described step 2 is that sterilized water adds at least three kinds of mixtures in bean cake powder, soyflour, W-Gum, bicarbonate of ammonia, molasses, potassium primary phosphate, the sal epsom again, and its weight percent is: bean cake powder 3%-5%, soyflour 2%-4%, W-Gum 1%-2%, bicarbonate of ammonia 0-0.3%, molasses 8%-14%, potassium primary phosphate 1.2%-3%, sal epsom 0-0.8%, sterilized water 71%-84%.
Solid enlarged culturing base in the described step 3 is that at least four kinds of mixtures that sterilized water adds in rice bran, soyflour, molasses, Semen Maydis powder, potassium primary phosphate, SODIUM PHOSPHATE, MONOBASIC, sal epsom, the garlic juice are again formed, and its weight percent proportioning is: rice bran 30%-35%, soyflour 20%-23%, molasses 8%-10%, Semen Maydis powder 7%-9%, potassium primary phosphate 0.5%-1%, SODIUM PHOSPHATE, MONOBASIC 1%-2%, sal epsom 0.3%-1%, garlic juice 0.5%-0.8%, sterilized water 18.2%-32.6%.
The present invention is made up of multiple beneficial microorganism (comprising bacterium and fungi), utilizes their phosphorus decomposing, potassium, degraded toxic substance, improves crop anti-adversity, promotes multiple efficacies such as soil ulmin formation.The carrier that also has special adsorption function simultaneously, can be in the medium-term and long-term survival of soil, can effectively improve the micro-ecological environment of crop root, promote the beneficial microorganism growth and breeding, suppress simultaneously and kill and wound to be harmful to humic bacterium or disease and pest, promote the normal development of root system, thereby promote plant growth to reduce or stop applying pesticides, reach a kind of safe, green planting effect, adopt the probiotics of normal growth in soil in the product, quantity is repaired modifying agent up to 1,000,000,000/g and the planting soil microbial ecological that contains the various active metabolic substd.After use, can form optimum ecotope in the crop soil, promote that crop transforms nutrient absorbing, improve Soil structure, suppress harmful growth of pathogenic bacteria and breeding, decompose the remaining agricultural chemicals in soil and the crop.
They can discharge the potassium of the invalid attitude of soil mineral and phosphorus for growth and development of plants and use to contain a lot of potassium bacteriums and phosphorus bacteria in the soil, the a lot of phosphate-solubilizing bacterias of report at present of the microbe species of phosphorus decomposing mainly contain bacillus (Bacillus) Rhodopseudomonas (Pseudomonas) Escherichia (Escherichia) etc., and the phosphorus decomposing ability of fungi generally is 10 times of bacterium.Thereby mechanism that it is generally acknowledged the microorganism phosphorus decomposing is that employing has the Bacillus licheniformis and the streptomycete of phosphate solubilization in the product of the present invention because microorganism secretion goes out these acid of organic acid can either reduce pH value and can combine with iron aluminium plasma again insoluble phosphate is dissolved.Bacillus licheniformis, saccharomyces cerevisiae also ferment in the present invention, and its quantity can be up to 10
9Individual/g.
Mycelia of some fungies and the root system of higher plant form a kind of association, are commonly called as " mycorhiza ", and it is that plant is in secular survival processes, with the common evolution results of mycorrhizal fungi.Its existence not only helps the existence of mycorrhizal fungi, also helps improving the resist ability of plant under poor environment, promotes plant-growth, improves the disease resistance that the plant rhizosphere environment strengthens plant, secretion growth hormone etc.Occurring in nature nearly all plant except that a few sections such as Cruciferae can both form mycorhiza.Mycorhiza mainly is divided into two kinds of ectomycorrhiza and endomycorrhizas, and endomycorrhiza is mainly by most kind of vesicles that form that belong in the Endogone, from dendritic mycorhiza (Vesiculararbuscularmycorrhiza) abbreviation VA mycorhiza.Product of the present invention in use can utilize soil ulmin and the pseudomonas combined action in fungies such as streptomycete, rhizopus and the soil, in root soil, form the mycelium of diameter 10-15cm effect, around root system of plant, set up radial network that a hypha,hyphae forms, network that these are formed by hypha,hyphae and the soil particle in the soil combine, formation is centered around plant stable soil microenvironment on every side, and then improving flowing of soil air, its effect and effect and mycorhiza are similar.Simultaneously, added a certain amount of natural garlic juice in the product of the present invention, its special smell can effectively be driven insect pest, contains a large amount of garlicins in the garlic juice simultaneously, is the important crude substance that suppresses the worm's ovum growth.
Because the discharging lack of standardization of life-time service chemical fertilizer and agricultural chemicals and some plant gas waste water and dregs, the organic-inorganic pollutent in the soil is increasing, and growth even poisonous plant that the fertility that has reduced soil suppresses plant simultaneously make it dead.Organic pesticide is some halogenated aryl hydrocarbons mostly, difficult degradation be some chlorinated hydrocarbon sterilants; As aldrin phenyl-hexachloride DDT Dieldrin-attapulgite mixture and phenoxy herbicides such as 24-D etc., some microorganisms in the soil then have the function of these organic pesticide of degraded, some microorganisms can be former in replacement with hydrogen the chlorine atom on the non-aromatic ring carbon, microorganism with this reduction dechlorination function has yeast proteus vulgaris serratia marcescens (Serratia marcescens), red flat Nocardia bacteria (Nocardia erythropolis), streptomycete and Aerobacter aerogenes (Aerobactteraerogenes) etc., and what adopt in the product of the present invention is saccharomyces cerevisiae and Streptomycin sulphate.
Soil microorganisms can impel the organism around the root system to form humic acid, organic matter in the soil is made up of the residue that is decomposing, the biological formed byproduct of undertaking decompose residues, microorganism itself and the stronger soil humic acid salt of resistance, Here it is soil ulmin, how many agrons is the most important sign of soil fertility size.Bacterium in the product of the present invention and fungi can both interact in normal soil and promote the formation of soil ulmin.It is generally acknowledged that nitrogen and carbohydrate in the humification compound partly are the plasmic chief components of microorganism.It is the humate that discharges from cell after microbial death.Containing a large amount of functional groups can improve the soil and can stimulate that increasing of humic acid directly promoting growth and development of plant in the plant growth soil.
Though above-mentioned different bacterium and fungi can play the part of a plurality of effects such as " phosphorus decomposings; potassium; degraded toxic substance; improve crop anti-adversity; promote soil ulmin " in planting soil, but microorganism is a kind of simple prokaryotic organism after all, its growth, pH value in the edatope is received in breeding, moisture, temperature, air content, Deng the suitable growth condition effect, the speed of growth of harmful simultaneously pathogenic bacteria such as putrefactive bacterium is faster than them, so the growing environment or the carrier of a safety are provided, it is the prerequisite of these beneficial microorganism effects of performance, some simple inorganicss both can be used as soil improvement agent and had used in soil in fact, can be used as the good carrier of beneficial microorganism again, the carrier among the present invention is by zeolite, wilkinite is formed.This class material has stronger adsorptive power, the dispersive grogs can be attached together, and forms soil agreegate, the effect of play water conservation, preserving moisture.
Advantage of the present invention is:
1. adopt and to promote the crop root growth and improve edatope multiple beneficial bacterium and fungi, reasonable compatibility, to promote crop root to grow, effects such as phosphorus decomposing, potassium, degraded toxic substance, raising crop anti-adversity, the formation of promotion soil ulmin organically combine;
2. adopt liquid single culture and solid complex ferment two-stage production technique, make effective microbe quantity reach 1,000,000,000/g, simultaneously its active metabolite is kept fully;
3. in liquid culture, add the promotor tween, in fermention medium, add natural pest-resistant harmful substances, can effectively reach the effect of pest control;
4. add carrier in product, the effect of existing absorption soil granular can allow beneficial microorganism and meta-bolites keep secular activity again, makes it bring into play permanent effect in soil;
5. adopting common grain crop and byproduct is main medium, and cost is low, product safety is reliable.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1
The first step. take by weighing bacterial classification: take by weighing each bacterial classification according to following weight part: 10 parts of Bacillus licheniformis, 6 parts of saccharomyces cerevisiaes, 3 parts of rhizopus, 2 parts of streptomycetes, above bacterial classification all can be bought in Chinese agriculture microbial strains preservation administrative center;
Second step. the liquid culture of Bacillus licheniformis, saccharomyces cerevisiae
A. mixed strains: the Bacillus licheniformis and the saccharomyces cerevisiae that take by weighing in the step 1 are mixed;
B. liquid culture: the ratio of mixed bacterial classification in 1: 25 is inoculated in the liquid nutrient medium of 125 ℃, 40 minutes high-temperature sterilizations, the tween (Tween) of interpolation 0.3% is done product promotor in the substratum, in fermentor tank, stirred 20 minutes, its rotating speed is 220 rev/mins, put into sterilization back plastic tank after stirring and left standstill 5 days, leave standstill the gas of measuring Ph value and release output in the process every day; Sampling is 3 times in the fermenting process, and microscopically detects the thalli growth situation and measures Ph value, stops to ferment when 1,000,000,000/ml concentration and Ph value reach 3.0-4.0 when total viable count reaches; The weight percent of liquid nutrient medium (%) ratio: bean cake powder 3, soyflour 2, W-Gum 1, molasses 10, potassium primary phosphate 1.2, sterilized water 82.8.
The 3rd step. the solid enlarged culturing of bacterial classification
A, with the bacterium liquid of the compound cultivation of step 2 mix the back by 1: 25 ratio access in the solid enlarged culturing base of 125 ℃, 4 minutes high-temperature sterilizations, add rhizopus and streptomycete simultaneously, stir 20 minutes, pour sealing and fermenting in the double-deck feedbag of sterilization into to evenly, 35 ℃ of temperature, 8 days time; Solid enlarged culturing basic weight amount per-cent (%) ratio: rice bran 30, soyflour 20, molasses 8, Semen Maydis powder 7, potassium primary phosphate 0.5, SODIUM PHOSPHATE, MONOBASIC 1, sal epsom 0.4, garlic juice 0.5, sterilized water 32.6.
Sampling is three times in b, the fermenting process, measures moisture and Ph value, takes a sample after the fermentation ends, and mensuration moisture is 35%, the Ph value is 5; Total viable count 1,000,000,000/g secondary fermentation finishes;
The 4th step. the dry carrier that adds
A. dry: the product of step 3 fermentation finished thoroughly1 is in chronological sequence put into the incubator drying, and temperature is controlled at 42-45 ℃, 10 hours time, and the material moisture content that drying is finished is controlled at≤and 25%;
B. add carrier: dried product is added carrier, carrier is that zeolite, wilkinite are formed, its weight proportion is: 40%: 60%, pour in the stirrer and stir, changing pulverizer then over to pulverizes, temperature of charge is controlled at below 45 ℃, and the material after the pulverizing is crossed 50 eye mesh screens, and sieve back coarse fodder is pulverized again;
The 5th step. packing
Product after sieving is incorporated with in the packing bag of individual event purging valve, preserves at dry, lucifuge place.
Embodiment 2
The first step. take by weighing bacterial classification: take by weighing each bacterial classification: 15 parts of Bacillus licheniformis, 8 parts of saccharomyces cerevisiaes, 5 parts of rhizopus, 3 parts of streptomycetes according to following weight part.
Second step. the liquid culture of Bacillus licheniformis, saccharomyces cerevisiae
A. mixed strains: with embodiment 1.
B. liquid culture: the ratio of mixed bacterial classification in 1: 30 is inoculated in the liquid nutrient medium of 130 ℃, 45 minutes high-temperature sterilizations, the tween (Tween) of interpolation 0.5% is done product promotor in the substratum, in fermentor tank, stirred 20 minutes, its rotating speed is 220 rev/mins, put into sterilization back plastic tank after stirring and left standstill 7 days, leave standstill the gas of measuring Ph value and release output in the process every day; Sampling is 3 times in the fermenting process, and microscopically detects the thalli growth situation and measures Ph value, stops to ferment when 1,000,000,000/ml concentration and Ph value reach 3.0-4.0 when total viable count reaches; Liquid nutrient medium weight percent (%) ratio: bean cake powder 5, soyflour 4, W-Gum 2, bicarbonate of ammonia 0.3, molasses 14, potassium primary phosphate 2, sal epsom 0.8, sterilized water 71.9.
The 3rd step. the solid enlarged culturing of bacterial classification
A. the ratio in 1: 30 after the bacterium liquid mixing of the compound cultivation of step 2 is inserted in the solid enlarged culturing base of 130 ℃, 45 minutes high-temperature sterilizations, all the other are with embodiment 1
B. with embodiment 1.
Solid enlarged culturing basic weight amount per-cent (%) ratio: rice bran 35, soyflour 23, molasses 10, Semen Maydis powder 9, potassium primary phosphate 1, SODIUM PHOSPHATE, MONOBASIC 2, sal epsom 1, garlic juice 0.8, sterilized water 18.2.
The 4th step. the dry carrier that adds
A. dry: with embodiment 1;
B. add carrier: dried product is added carrier, carrier is that zeolite, wilkinite are formed, its weight percent proportioning is: 60%: 40%, pour in the stirrer and stir, changing pulverizer then over to pulverizes, temperature of charge is controlled at below 45 ℃, and the material after the pulverizing is crossed 50 eye mesh screens, and sieve back coarse fodder is pulverized again;
The 5th step. packing: with embodiment 1.
In use, according to the different soils condition, product can use separately by different ratios, or mix the back with fertilizer and use, sowing or transplant before, by the quantity of about five grams of every strain plant this product is evenly placed bar ditch or hole bottom, on cover and loosen the soil about five centimeters, can sow or transplant, the relatively more serious plot of preceding stubble disease and pest is taken the circumstances into consideration dosage and is used.When using base manure, also this product evenly can be sneaked into organic fertilizer in 2 liters every mu ratio and use.Attention this product need be deposited in the hermetically drying place, avoid mixing stacking with agricultural chemicals, strong stimulation article etc., use as early as possible behind the Kaifeng, this product agriculture garden that advances in Songjiang, Shanghai after research and development are finished carries out crop simultaneous tests such as cucumber, tomato and finds, this biological soil fertilizer uses ensuing crop output to improve 12-17%, root length on average increases 3-8cm, and crop pest reduces 40-45%, does not re-use agricultural chemicals substantially.
Claims (6)
1. a complex microorganism preparations that promotes root system growing and reducing disease is characterized in that, is made up of following weight part raw material: 40 parts-60 parts of microbial strain culture, 60 parts-40 parts in beneficial microorganism carrier.
2. a kind of complex microorganism preparations that promotes root system growing and reducing disease according to claim 1, it is characterized in that the bacterial classification of described microbial strain culture is made up of following weight part raw material: 10 parts-15 parts of Bacillus licheniformis, 6 parts-8 parts of saccharomyces cerevisiaes, 3 parts-5 parts of rhizopus, 2 parts-3 parts of streptomycetes.
3. a kind of complex microorganism preparations that promotes root system growing and reducing disease according to claim 1 is characterized in that, described beneficial microorganism carrier is zeolite, bentonitic a kind of or its mixture.
4. a preparation method who promotes the complex microorganism preparations of root system growing and reducing disease is characterized in that, making processes of the present invention is:
The first step. take by weighing bacterial classification: take by weighing each bacterial classification: 10 parts-15 parts of Bacillus licheniformis, 6 parts-8 parts of saccharomyces cerevisiaes, 3 parts-5 parts of rhizopus, 2 parts-3 parts of streptomycetes according to following weight part;
Second step. the liquid culture of Bacillus licheniformis, saccharomyces cerevisiae
A. mixed strains: the Bacillus licheniformis and the saccharomyces cerevisiae that take by weighing in the step 1 are mixed;
B. liquid culture: liquid nutrient medium 120 ℃-130 ℃, through 30 minutes-45 minutes high-temperature sterilizations, in substratum, add the tween of 0.3%-0.5%, with mixed bacterial classification in 1: the ratio of 25-30 is inoculated in the liquid nutrient medium, in fermentor tank, stirred 20 minutes, its rotating speed is 220-240 rev/min, put into sterilization back plastic tank after stirring and left standstill 5-7 days, leave standstill the gas of measuring Ph value and release output in the process every day; Sampling is 3 times in the fermenting process, and microscopically detects the thalli growth situation and measures Ph value, stops to ferment when 1,000,000,000/ml concentration and Ph value reach 3.0-4.0 when total viable count reaches;
The 3rd step. the solid enlarged culturing of bacterial classification
A. with the bacterium liquid of the compound cultivation of step 2 in 1: the ratio of 25-30 inserts in the solid enlarged culturing base of 120 ℃ of-130 ℃, 30 minutes-45 minutes high-temperature sterilizations, add rhizopus in the step 1, streptomycete simultaneously, stir 20 minutes-30 minutes to even, pour sealing and fermenting in the double-deck feedbag of sterilization into, temperature is 30 ℃-35 ℃, 7 days-9 days time;
B. take a sample three times in the fermenting process, measure moisture and Ph value, take a sample after the fermentation ends, mensuration moisture is that 35%-40%, Ph value are 4.5-6.0; Total viable count 1,000,000,000/g secondary fermentation finishes;
The 4th step. the dry carrier that adds
A. dry: the product of step 3 fermentation finished thoroughly1 is in chronological sequence put into the incubator drying, and temperature is controlled at 42 ℃-45 ℃, 10 hours-12 hours time, and the material moisture content that drying is finished is controlled at≤and 25%;
B. add carrier: dried microbial strain culture is added carrier, its weight part is: microbial strain culture 40-60%, carrier 60-40%, pour in the stirrer and stir, changing pulverizer then over to pulverizes, temperature of charge is controlled at below 45 ℃, material after the pulverizing is crossed 50 eye mesh screens, and sieve back coarse fodder is pulverized again;
The 5th step. packing
Product after sieving is incorporated with in the packing bag of individual event purging valve, preserves at dry, lucifuge place.
5. a kind of preparation method who promotes the complex microorganism preparations of root system growing and reducing disease according to claim 4, it is characterized in that, liquid nutrient medium in the described step 2 is that sterilized water adds at least three kinds of mixtures in bean cake powder, soyflour, W-Gum, bicarbonate of ammonia, molasses, potassium primary phosphate, the sal epsom again, and its weight percent is: bean cake powder 3%-5%, soyflour 2%-4%, W-Gum 1%-2%, bicarbonate of ammonia 0-0.3%, molasses 8%-14%, potassium primary phosphate 1.2%-3%, sal epsom 0-0.8%, sterilized water 71%-84%.
6. a kind of preparation method who promotes the complex microorganism preparations of root system growing and reducing disease according to claim 4, it is characterized in that, solid enlarged culturing base in the described step 3 is that sterilized water adds rice bran again, soyflour, molasses, Semen Maydis powder, potassium primary phosphate, SODIUM PHOSPHATE, MONOBASIC, sal epsom, at least four kinds of mixtures in the garlic juice are formed, and its weight percent proportioning is: rice bran 30%-35%, soyflour 20%-23%, molasses 8%-10%, Semen Maydis powder 7%-9%, potassium primary phosphate 0.5%-1%, SODIUM PHOSPHATE, MONOBASIC 1%-2%, sal epsom 0.3%-1%, garlic juice 0.5%-0.8%, sterilized water 18.2%-32.6%.
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| CN104839228A (en) * | 2015-04-17 | 2015-08-19 | 赵德邦 | Crop yield increasing agent and preparation process thereof |
| CN111587895A (en) * | 2019-02-19 | 2020-08-28 | 安徽省农业科学院蚕桑研究所 | Biological bactericide for preventing and treating mulberry ring spot and preparation method thereof |
| CN112640755A (en) * | 2021-01-20 | 2021-04-13 | 中国农业科学院农业资源与农业区划研究所 | Method for transplanting tobacco seedlings by using mycelium nutrients |
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