CN109906842A - Application of the super quick albumen complex enzyme formulation in prevention and treatment yellow twig - Google Patents
Application of the super quick albumen complex enzyme formulation in prevention and treatment yellow twig Download PDFInfo
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- CN109906842A CN109906842A CN201910194471.3A CN201910194471A CN109906842A CN 109906842 A CN109906842 A CN 109906842A CN 201910194471 A CN201910194471 A CN 201910194471A CN 109906842 A CN109906842 A CN 109906842A
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- 102000004190 Enzymes Human genes 0.000 title claims abstract description 92
- 108090000790 Enzymes Proteins 0.000 title claims abstract description 92
- 239000000203 mixture Substances 0.000 title claims abstract description 45
- 238000009472 formulation Methods 0.000 title claims abstract description 32
- 230000002265 prevention Effects 0.000 title claims abstract description 10
- 229940088598 enzyme Drugs 0.000 claims abstract description 91
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 25
- 239000004365 Protease Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229910021536 Zeolite Inorganic materials 0.000 claims abstract description 22
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000000843 powder Substances 0.000 claims abstract description 22
- 239000010457 zeolite Substances 0.000 claims abstract description 22
- 239000008103 glucose Substances 0.000 claims abstract description 21
- 108010004032 Bromelains Proteins 0.000 claims abstract description 16
- 235000019835 bromelain Nutrition 0.000 claims abstract description 16
- 102000004882 Lipase Human genes 0.000 claims abstract description 15
- 108090001060 Lipase Proteins 0.000 claims abstract description 15
- 239000004367 Lipase Substances 0.000 claims abstract description 15
- 235000019421 lipase Nutrition 0.000 claims abstract description 15
- 108090000526 Papain Proteins 0.000 claims abstract description 14
- 229940055729 papain Drugs 0.000 claims abstract description 14
- 235000019834 papain Nutrition 0.000 claims abstract description 14
- 102000019197 Superoxide Dismutase Human genes 0.000 claims abstract description 13
- 108010012715 Superoxide dismutase Proteins 0.000 claims abstract description 13
- 238000000855 fermentation Methods 0.000 claims abstract description 11
- 230000004151 fermentation Effects 0.000 claims abstract description 11
- 238000002360 preparation method Methods 0.000 claims abstract description 9
- 239000004382 Amylase Substances 0.000 claims abstract description 6
- 102000013142 Amylases Human genes 0.000 claims abstract description 6
- 108010065511 Amylases Proteins 0.000 claims abstract description 6
- 235000019418 amylase Nutrition 0.000 claims abstract description 6
- 238000010564 aerobic fermentation Methods 0.000 claims description 12
- 229920002472 Starch Polymers 0.000 claims description 10
- 230000005070 ripening Effects 0.000 claims description 10
- 239000008107 starch Substances 0.000 claims description 10
- 235000019698 starch Nutrition 0.000 claims description 10
- QGMRQYFBGABWDR-UHFFFAOYSA-M Pentobarbital sodium Chemical compound [Na+].CCCC(C)C1(CC)C(=O)NC(=O)[N-]C1=O QGMRQYFBGABWDR-UHFFFAOYSA-M 0.000 claims description 7
- 229960001780 bromelains Drugs 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 229920002678 cellulose Polymers 0.000 claims description 5
- 239000001913 cellulose Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 238000006213 oxygenation reaction Methods 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 3
- 108010059892 Cellulase Proteins 0.000 abstract description 11
- 229940106157 cellulase Drugs 0.000 abstract description 11
- 241000207199 Citrus Species 0.000 abstract description 7
- 235000020971 citrus fruits Nutrition 0.000 abstract description 7
- 241001478315 Candidatus Liberibacter asiaticus Species 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- 230000009471 action Effects 0.000 abstract description 2
- 238000000354 decomposition reaction Methods 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 239000002689 soil Substances 0.000 description 6
- 235000013399 edible fruits Nutrition 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- -1 oligosaccharidase Proteins 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 244000189799 Asimina triloba Species 0.000 description 2
- 235000006264 Asimina triloba Nutrition 0.000 description 2
- 235000009467 Carica papaya Nutrition 0.000 description 2
- 108091005804 Peptidases Proteins 0.000 description 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 238000006555 catalytic reaction Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 235000019419 proteases Nutrition 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000003421 catalytic decomposition reaction Methods 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 241001233061 earthworms Species 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002015 leaf growth Effects 0.000 description 1
- 239000002068 microbial inoculum Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229920001542 oligosaccharide Polymers 0.000 description 1
- 150000002482 oligosaccharides Chemical class 0.000 description 1
- 239000002420 orchard Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0155—Litter comprising organic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
- A01K1/0152—Litter
- A01K1/0154—Litter comprising inorganic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/28—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture specially adapted for farming
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Organic Chemistry (AREA)
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Abstract
The present patent application is 201811529411.4 divisional application.The invention discloses application of the super quick albumen complex enzyme formulation in prevention and treatment yellow twig, belong to enzyme preparation technical field.The albumen complex enzyme formulation is prepared by complex enzyme, zeolite powder, glucose and water by fermentation；The complex enzyme is by superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain composition.The albumen complex enzyme formulation has very Johnson & Johnson's object catalyticing decomposition action, also has the effect of preventing and treating yellow twig, is able to solve citrus and easily occurs the problem of yellow twig in planting process, the prevention and treatment suitable for Citrus Huanglongbing pathogen.
Description
The application is application number 201811529411.4,13 days December 2018 applying date, a kind of denomination of invention " super quick egg
The divisional application of white complex enzyme formulation and the preparation method and application thereof ".
Technical field
The present invention relates to application of the super quick albumen complex enzyme formulation in prevention and treatment yellow twig, belong to enzyme preparation technical field.
Background technique
Easily there is yellow twig in planting process in citrus, brings loss, therefore the prevention and treatment pair of Citrus Huanglongbing pathogen to peasant household
It is of great significance in citrus plantation.
Summary of the invention
To solve the problems, such as that yellow twig easily occurs in citrus, the present invention provides super quick albumen complex enzyme formulations in prevention and treatment Huanglong
Application in disease, the technical solution adopted is as follows:
The present invention provides application of the albumen complex enzyme formulation in prevention and treatment yellow twig, especially in prevention and treatment Citrus Huanglongbing pathogen
In application, the albumen complex enzyme formulation is prepared by complex enzyme, zeolite powder, glucose and water by fermentation；The complex enzyme
By superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain composition.
Preferably, the complex enzyme, zeolite powder, glucose and water are matched according to following parts by weight: 5.72 parts by weight~
8.82 parts by weight complex enzyme；3.8 parts by weight~5.7 parts by weight zeolite powders；0.39 parts by weight~1.14 parts by weight glucose；7.6
Parts by weight~11.4 parts by weight water；Wherein: 5.72 parts by weight~8.82 parts by weight complex enzymes are by 0.1 parts by weight~0.15 weight
Amount part superoxide dismutase, 1.5 parts by weight~2.5 parts by weight bromelains, 2 parts by weight~3 part by weight of cellulose enzymes, 1
Parts by weight~1.5 parts by weight lipase, 1 parts by weight~1.5 weight starch enzymes, 0.05 parts by weight~0.07 parts by weight oligosaccharide
Enzyme and 0.07 parts by weight~0.1 parts by weight papain composition.
It is highly preferred that the complex enzyme, zeolite powder, glucose and water are matched according to following parts by weight: complex enzyme 7.26
Parts by weight；4.2 parts by weight of zeolite powder；0.78 parts by weight of glucose；9.4 parts by weight of water；Wherein: the 7.26 parts by weight complex enzyme
By 0.12 parts by weight superoxide dismutase, 2 parts by weight bromelains, 2.5 part by weight of cellulose enzymes, 1.3 parts by weight fat
Enzyme, 1.2 weight starch enzymes, 0.06 parts by weight oligosaccharidase and 0.08 parts by weight papain composition.
Preferably, the preparation method of the albumen complex enzyme formulation is by complex enzyme, and oxygenation stirs after zeolite powder and water mixing
It mixes uniformly, aerobic fermentation 10-12 days under conditions of 35 DEG C -37 DEG C, pH are 5.7, then adjusts pH to 6.5-6.8, Portugal is added
Grape sugar continues aerobic fermentation and carries out the cultivation of after ripening phase to the 45-50 days, stands after the completion of cultivating, filters to obtain albumen complex enzyme
Preparation.
It is highly preferred that the number of days of the aerobic fermentation is 10 days.
It is stirred once every 15h during fermentation it is highly preferred that the after ripening phase cultivates, stirs 30min every time.
It is highly preferred that the time of the standing is 45 days.
Super quick albumen complex enzyme formulation of the invention is a kind of super quick compound enzyme product of albumen, is existed with protein form, no
It is microbial inoculum, which has powerful biocatalysis decomposition, passes through beneficial microbe in catalysis biological body
Positive reaction, catalysis inhibits harmful microbe negative reaction in organism, realizes that " Tiny ecosystem " is in balance, can activated cell it is living
Property, repel foreign protei, substantially enhance the immunity energy of organism, the albumen complex enzyme formulation stability is very strong, anti-alien bacteria
Interference performance is strong, not by the infringement of alien species, has bio-diversity, the fast feature of catalytic.
The invention has the advantages that:
The present invention provides a kind of albumen complex enzyme formulation, which there is very Johnson & Johnson's object catalytic decomposition to make
With.Albumen complex enzyme formulation of the present invention also has the effect of preventing and treating yellow twig.
Specific embodiment
The present invention will be further described combined with specific embodiments below, but the present invention should not be limited by the examples.
Embodiment 1:
A kind of albumen complex enzyme formulation is present embodiments provided, the albumen complex enzyme formulation is by complex enzyme, zeolite powder, grape
Sugar and water by fermentation are prepared；The complex enzyme is by superoxide dismutase, bromelain, cellulase, lipase, starch
Enzyme, oligosaccharidase and papain composition；Wherein:
5.72 parts by weight complex enzyme；3.8 parts by weight zeolite powders；0.39 parts by weight glucose；7.6 parts by weight water；
5.72 parts by weight complex enzymes are by 0.1 parts by weight superoxide dismutase, 1.5 parts by weight bromelains, 2 parts by weight
Cellulase, 1 parts by weight lipase, 1 weight starch enzyme, 0.05 parts by weight oligosaccharidase and 0.07 parts by weight papain
Composition.
The present embodiment additionally provides a kind of preparation method of above-mentioned albumen complex enzyme formulation, which is by super oxygen
Object mutase, bromelain, cellulase, lipase, amylase, oligosaccharidase, papain, zeolite powder, glucose
With water mixing after oxygenation stir evenly, 35 DEG C -37 DEG C, pH be 5.7 under conditions of aerobic fermentation 10 days after be added glucose after
Continue aerobic fermentation to the 45th day, then adjusts pH to 6.5-6.8 and carry out the cultivation of after ripening phase, stirred during fermentation every 15h
Once, 30min is stirred every time, is stood 45 days after the completion of the after ripening phase cultivates, is filtered to obtain albumen complex enzyme formulation.
Embodiment 2
A kind of albumen complex enzyme formulation is present embodiments provided, the albumen complex enzyme formulation is by complex enzyme, zeolite powder, grape
Sugar and water by fermentation are prepared；The complex enzyme is by superoxide dismutase, bromelain, cellulase, lipase, starch
Enzyme, oligosaccharidase and papain composition；Wherein:
8.82 parts by weight complex enzyme；5.7 parts by weight zeolite powders；1.14 parts by weight glucose；11.4 parts by weight water；
8.82 parts by weight complex enzymes are by 0.15 parts by weight superoxide dismutase, 2.5 parts by weight bromelains, 3 weight
Part cellulase, 1.5 parts by weight lipase, 1.5 weight starch enzymes, 0.07 parts by weight oligosaccharidase and 0.1 parts by weight pawpaw
Protease composition.
The present embodiment additionally provides a kind of preparation method of above-mentioned albumen complex enzyme formulation, which is by super oxygen
Object mutase, bromelain, cellulase, lipase, amylase, oligosaccharidase, papain, zeolite powder, glucose
With water mixing after oxygenation stir evenly, 35 DEG C -37 DEG C, pH be 5.7 under conditions of aerobic fermentation 10 days after be added glucose after
Continue aerobic fermentation to the 45th day, then adjusts pH to 6.5-6.8 and carry out the cultivation of after ripening phase, stirred during fermentation every 15h
Once, 30min is stirred every time, is stood 45 days after the completion of the after ripening phase cultivates, is filtered to obtain albumen complex enzyme formulation.
Embodiment 3
A kind of albumen complex enzyme formulation is present embodiments provided, the albumen complex enzyme formulation is by complex enzyme, zeolite powder, grape
Sugar and water by fermentation are prepared；The complex enzyme is by superoxide dismutase, bromelain, cellulase, lipase, starch
Enzyme, oligosaccharidase and papain composition；Wherein:
7.26 parts by weight of complex enzyme；4.2 parts by weight of zeolite powder；0.78 parts by weight of glucose；9.4 parts by weight of water；
7.26 parts by weight complex enzymes are by 0.12 parts by weight superoxide dismutase, 2 parts by weight bromelains, 2.5 weight
Part cellulase, 1.3 parts by weight lipase, 1.2 weight starch enzymes, 0.06 parts by weight oligosaccharidase and 0.08 parts by weight pawpaw
Protease composition.
The present embodiment additionally provides a kind of preparation method of above-mentioned albumen complex enzyme formulation, which is by super oxygen
Object mutase, bromelain, cellulase, lipase, amylase, oligosaccharidase, papain, zeolite powder, glucose
With water mixing after oxygenation stir evenly, 35 DEG C -37 DEG C, pH be 5.7 under conditions of aerobic fermentation 10 days after be added glucose after
Continue aerobic fermentation to the 45th day, then adjusts pH to 6.5-6.8 and carry out the cultivation of after ripening phase, stirred during fermentation every 15h
Once, 30min is stirred every time, is stood 45 days after the completion of the after ripening phase cultivates, is filtered to obtain albumen complex enzyme formulation.
In order to verify albumen complex enzyme to sugar orange yellow twig control efficiency, Xing Huazhen, Jiangkou town in Guangdong Province Fengkai County
It is tested.3 albumen complex enzyme formulation of embodiment is mixed with water according to mass ratio for 1:300, it is compound to be configured to albumen
Enzyme solutions make albumen composite enzyme solution prepared with embodiment 3 to sugar orange fruit tree Baconic, observe hair root situation, and observe soil
Earth improves organic matter and increases situation.The variation of wood louse survival volume is checked and examined after spraying blade face with albumen composite enzyme solution, observes the spring tip
Growth change and control shoot effect observe passages through which vital energy circulates and blade variation, measure the amount of blooming and fruit-setting rate, observe branch blade, fruit hair
Educate whether normally equal every detection, observation, evaluation work.Testing mu number is 9 mu.
Test result: the soil organism increases after soil improvement, and soil property is soft, increases gas permeability, and harmful virus becomes extinct, and three
Soil restoration life after a month has in soil many earthworms to occur.Main root restores life, it is seen that a large amount of hair roots.Shoot and leaf growth, leaf
Piece increases, and greening, fruit-setting rate greatly improves.After complex enzyme 1 year, fruit-setting rate is improved, autumn completely filled fruit, and moisture is sufficient,
Sugar increases, and does not find yellow twig virus after detecting.Complex enzyme obtains in administering sugar orange yellow twig experimentation can
Like achievement, plant under the action of complex enzyme, grow thickly by root system, flourishing, and fruit-setting rate restores very fast, completely filled fruit, sugar compared with
Height, taste is preferable, the commendation by local orchard worker.
Although the present invention has been disclosed in the preferred embodiment as above, it is not intended to limit the invention, any to be familiar with this
The people of technology can do various changes and modification, therefore protection of the invention without departing from the spirit and scope of the present invention
Range should subject to the definition of the claims.
Claims (7)
1. application of the albumen complex enzyme formulation in prevention and treatment yellow twig, which is characterized in that the albumen complex enzyme formulation is by compound
Enzyme, zeolite powder, glucose and water by fermentation are prepared；The complex enzyme is by superoxide dismutase, bromelain, cellulose
Enzyme, lipase, amylase, oligosaccharidase and papain composition.
2. application according to claim 1, which is characterized in that the complex enzyme, zeolite powder, glucose and water are according to as follows
Parts by weight proportion: 5.72 parts by weight~8.82 parts by weight complex enzymes；3.8 parts by weight~5.7 parts by weight zeolite powders；0.39 weight
Part~1.14 parts by weight glucose；7.6 parts by weight~11.4 parts by weight water；Wherein: 5.72 parts by weight~8.82 parts by weight
Complex enzyme is by 0.1 parts by weight~0.15 parts by weight superoxide dismutase, 1.5 parts by weight~2.5 parts by weight bromelains, and 2
Parts by weight~3 part by weight of cellulose enzymes, 1 parts by weight~1.5 parts by weight lipase, 1 parts by weight~1.5 weight starch enzymes,
0.05 parts by weight~0.07 parts by weight oligosaccharidase and 0.07 parts by weight~0.1 parts by weight papain composition.
3. application according to claim 2, which is characterized in that the complex enzyme, zeolite powder, glucose and water are according to as follows
Parts by weight proportion: 7.26 parts by weight of complex enzyme；4.2 parts by weight of zeolite powder；0.78 parts by weight of glucose；9.4 parts by weight of water；
Wherein: the 7.26 parts by weight complex enzyme is by 0.12 parts by weight superoxide dismutase, 2 parts by weight bromelains, and 2.5
Part by weight of cellulose enzyme, 1.3 parts by weight lipase, 1.2 weight starch enzymes, 0.06 parts by weight oligosaccharidase and 0.08 parts by weight
Papain composition.
4. application according to claim 1 to 3, which is characterized in that the preparation method of the albumen complex enzyme formulation is
By complex enzyme, oxygenation is stirred evenly after zeolite powder and water mixing, aerobic fermentation 10- under conditions of 35 DEG C -37 DEG C, pH are 5.7
12 days, then adjust pH to 6.5-6.8, be added glucose continue aerobic fermentation carry out the cultivation of after ripening phase to the 45-50 days, to
It is stood after the completion of cultivating, filters to obtain albumen complex enzyme formulation.
5. application according to claim 4, which is characterized in that the number of days of the aerobic fermentation is 10 days.
6. application according to claim 4, which is characterized in that the after ripening phase cultivates and stirs during fermentation every 15h
It mixes once, stirs 30min every time.
7. application according to claim 4, which is characterized in that the time of the standing is 45 days.
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| CN201910194471.3A CN109906842B (en) | 2018-12-13 | 2018-12-13 | Application of hypersensitive protein complex enzyme preparation in preventing and treating huanglongbing |
| CN201811529411.4A CN109618944B (en) | 2018-12-13 | 2018-12-13 | Hypersensitive protein complex enzyme preparation as well as preparation method and application thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109618944A (en) * | 2018-12-13 | 2019-04-16 | 哈尔滨森荞生物科技有限公司 | Hypersensitive protein complex enzyme preparation and preparation method and application thereof |
| CN110800761A (en) * | 2019-11-01 | 2020-02-18 | 潍坊科技学院 | Signal protein-containing composition for preventing and treating root-knot nematode as well as preparation method and application thereof |
| CN115282264A (en) * | 2022-08-31 | 2022-11-04 | 北京志宣生物科技有限公司 | Compound preparation for reducing blood sugar and improving immunity and preparation method thereof |
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| CN109618944A (en) | 2019-04-16 |
| CN109618944B (en) | 2022-05-13 |
| CN109906842B (en) | 2022-03-11 |
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