CN102258017A - Photosynthetic bacterium detoxification yield increasing agent and production method thereof - Google Patents
Photosynthetic bacterium detoxification yield increasing agent and production method thereof Download PDFInfo
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Abstract
The invention relates to a photosynthetic bacterium detoxification yield increasing agent which is a viscous brown liquid. The liquid contains a microorganism complexing agent of three strains PSB-B4, PSB-1 and PSB-4 of photosynthetic bacteria (PSB); and the ratio of the three strains is 1: 1: 1 based on weight of powder when preparation is carried out, the content of effective viable bacterium of photosynthetic bacteria in the liquid is 500 million pcs/g, the content of a synergistic material in the liquid is 0.1-2.0% based on the weight of the liquid as a reference, and the synergistic material is a substance capable of promoting crop growth and enhance antiretroviral capability. The photosynthetic bacterium detoxification yield increasing agent provided by the invention is suitably used as a plant detoxification yield increasing agent.
Description
Technical field
The present invention relates to complex micro organism fungicide and give birth to main method, especially supply the complex micro organism fungicide and the production method thereof of plant absorbing.
Background technology
Over nearly 20 years, chemical fertilizer industry sharply develops, and a large amount of inorganic fertilizer, chemical pesticides are manured into soil, agricultural produce has been played important function, but also caused simultaneously environmental pollution, grain, really, vegetable chemistry medicament residual quantity increases, the soil salinization, harden, adverse consequences such as fertility depletion.Especially crops, vegetables, fruit residue of pesticide exceed standard, and have a strong impact on the foreign exchange earning of people's healthy and agricultural product.Utilize the remains of pesticide in the biotechnology degraded soil, reduce the residue of pesticide of crops, vegetables, fruit and cultivated plant, produce the technology of nuisanceless green environment-friendly products, also rarely seen report.Photosynthetic bacteria (PhotosyntheticBacteria Abbr.name is called for short PSB) is to utilize luminous energy and carbonic acid gas to keep coloured bacterium of autotrophy life, at the occurring in nature ubiquity, be the general name of under anaerobic not putting the photosynthetic bacterium of oxygen, being a class carries out photosynthetic microorganism as the energy, the organic matter that can utilize occurring in nature under anaerobism illumination or aerobic dark condition, sulphide, ammonia etc. as the hydrogen donor carbon source of holding concurrently with light.Photosynthetic bacteria is distributed widely in natural soil, paddy field, marsh, lake, Jiang Hai etc. to be located, and mainly is distributed in the anoxic zone that the light transmissive arrives in the aquatic environment.Photosynthetic bacterial thallus is rich in the ubiquinone of amino acid, vitamin, higher concentration
10Etc. several physiological active substances and vitamin B group, especially Cobastab quite completely
12Content is 21%, protein content reaches 60%～65%, folic acid and vitamin h content are high especially, vitamin h content is 65%, also has bacteriochlorin, carotenoid, and some active bacterial strains also contain antiviral substance, even component also has spread effect to rhizobia outside the born of the same parents of thalline secretion, can significantly improve its nitrogenase activity.At natural world, photosynthetic bacteria carbon, nitrogen fix and the sulfur cycle of soil in play an important role.It is improving plant nutrition, increase soil fertility and is suppressing all to play an important role aspect the pathogenic microorganism growth.Have the growth of promotion, improve the effect of quality and volume increase.Have the formation that promotes root system and plant development, promotion bud and fruit, the effects such as validation that promote jarovize, promotion soil organic matter simultaneously.In addition, research thinks that also PSB can promote beneficial microorganism propagation, has the ability that suppresses pathogen, remains of pesticide, H in the soil of also can effectively degrading
2S, superfluous ammonia nitrogen etc., and can alleviate poisoning to a certain extent, so PSB is a good soil conditioner pollution-free, nuisanceless, that can purify soil.
Photosynthetic bacteria is as a kind of bio-fertilizer, and the application on agricultural more and more widely has some manufacturer production photosynthetic bacteria bacterial manure both at home and abroad and also puts goods on the market.In the world photosynthetic bacteria bacterial manure is worked out corresponding standard, it will have very big development prospect as microbial manure on agricultural.The Chinese patent application file of publication number CN_1145408A has been announced " a kind of bacterium for degradating residual agricultural chemical and production method thereof ", but because employed bacterial strain is the bacterial strain of the called after J# one [5] of bud pole Pseudomonas, thereby this product is mainly just better to removing the methamidophos residue effect, and application surface is limited.And at present in agricultural and gardening production, generally the agricultural chemicals of Shi Yonging also has multiple organophosphorus insecticide, chrysanthemum ester insecticide, carbamate insecticide and dicofol class except that orthene, and weed killer herbicide, bactericide or the like.Publication number provides a kind of bacterium for degradating residual agricultural chemical and production method thereof for the CN1386845A patent application, this microbial inoculum is a kind of single residual microbial inoculum that falls, after directly being applied to the crops of the various chemical pesticides of normal use in process of growth, can make the low respectively 20-70% of the residual residue of pesticide index that limits than country of agricultural chemicals such as hexachlorcyclohexane, DDT, cyanogen penta chrysanthemum vinegar, dicofol and acephatemet.But this product only has single degrading pesticide residues effect, without any synergistic matter, and degrade residual speed is too slow, bacterial strain falls behind and is single, and degradation is limited, and what can degrade all is the pesticide species that country eliminates, and vast farmers do not separated harmful restitution because of using the improper poisoning that causes of agricultural chemicals not have yet, can not regulate growth, improve output, also not possess effect with mixture of herbicides performance synergy, safety, low toxicity.
Study on brassinolide (BR) is by people such as U.S. doctor Mirchell 1970 isolated steroidal substances from rape pollen at first, it has strong facilitation to the elongation and the cell division of axis, and this discovery was subjected to plant physiology circle and relevant subject expert's extensive attention at that time.Study on brassinolide is called as the sixth-largest parahormone.Result of practical application shows that as a kind of novel steroidal plant hormone, study on brassinolide has effects such as promoting plant growth, the effect that increases crop yield, improves crop anti-adversity, alleviation poisoning, recovery growth, can improve the output of crops.Compound sodium nitrophenolate is to be mixed by a certain percentage by 5 nitroguaiacol sodium, sodium onitrophenol, three kinds of monomers of Sodium p-nitrophenoxide, every kind of monomer is brought into play not same-action, the energy rapid permeability is in plant corpus behind three's science compatibility, promote cellular plasm to flow, improve cell viability, promote robust growth.Because it has efficiently, low toxicity, noresidue, applicable crops are wide, have no side effect, the working concentration scope greatly, be applicable to an advantage such as cut plant, apply more than 40 countries and regions in the world.Tea polysaccharide is a kind of acidoglycoprotein, is combined with a large amount of mineral elements, is called as the tealeaves polysaccharide compound, is called for short tealeaves polysaccharide or tea polysaccharide (Tea Polysaccharide).Wherein protein part mainly is made up of about 20 kinds of common amino acid, and the part of sugar is mainly by arabinose, wood sugar, fucose, glucose, galactose etc., and mineral element mainly reaches a spot of trace element, rare earth element etc. by calcium, magnesium, iron, manganese etc.Tea polysaccharide has good effect to promoting crop internal metabolism, harmonic growth to grow, alleviate poisoning.Diethyl aminoethyl hexanoate (being called for short DA-6) is a kind of new plant growth regulator, it can promote absorption and the utilization of crop to the multiple nutrients composition, regulate growth, detoxifcation more wound, volume increase, degeneration-resistant, disease-resistant, to improve effects such as quality, precocity remarkable, and have efficient, wide spectrum, the characteristics of working concentration wide ranges.The polysaccharide that alginic acid is made up of D-mannuronic acid and L-guluronic acid, normality is a pale yellow powder, and suspending, thickening, emulsification, effect such as bonding are arranged.The alginic acid raw material is from natural wild marine alga, kept the distinctive pure natural physiological activator of marine alga, easily absorbed, have the flower bud differentiation of the crop of promotion, improve and sit flower, fruit-setting rate with precocious by crop, optimization fruit mouthfeel,, increase the effect of fresh keeping time and anti-accumulating, can also strengthen various crop disease-resistants, anti-adversity ability, realize that yellow leaf speed is green, leaflet becomes big, pre-anti-fall stem, leaf, flower, really have the effect of the dead seedling of prevention, mashed root.Xylo-oligosaccharide is with β-1 by 2-7 wood sugar molecule, the functional polymerization sugar of 4 glycosidic bond be combined intos, compare with the used soyabean oligosaccharides of common people, fructo-oligosaccharide (FOS), oligoisomaltose etc. and to have special advantages, its bifidobacterium factor functional be other polymerization carbohydrate 10-20 doubly, to improve resistance against disadvantage, firm cell membrane, improve adaptability, alleviate poisoning agricultural chemicals, recovering to grow vigorously after the poisoning has unique effect.
Compare with prior art, the invention belongs to a kind of safener, antipoison, volume increase agent, fall residual dose
Summary of the invention
The objective of the invention is photosynthetic microbial inoculum (PSB) and synergistic component are prepared into detoxifcation volume increase agent, this detoxifcation volume increase agent belongs to a plant nutrient liquor.
The present invention includes the production method of a kind of photosynthetic bacteria detoxifcation volume increase agent and this volume increase agent.
A kind of photosynthetic bacteria detoxifcation volume increase agent of the present invention, its special character is:
Described detoxifcation volume increase agent is thick brown liquid, and this liquid contains photosynthetic bacteria and belongs to (Photosynthetic Bacteria, the microorganism complex of PSB-B4 PSB), PSB-1, three kinds of bacterial strains of PSB-4; The ratio of described three kinds of bacterial strains with when preparation pulvis weight count 1: 1: 1, effective viable bacteria content of photosynthetic bacteria is 500,000,000 a/gram in the described liquid, with described liquid weight is that synergistic matter content is 0.1 in this liquid of benchmark---2.0%, and described synergistic matter is meant a class material of the enhancing anti-adversity ability that can promote plant growth.
The production method of photosynthetic bacteria detoxifcation volume increase agent of the present invention, its special character is may further comprise the steps:
(1) described three kinds of bacterial strain pulvis being mixed with 1: 1: 1 part by weight, is that the 0.3-2% of medium material gross weight is inoculated in the seed culture case and cultivates by the bacterial classification inoculation amount, is benchmark with medium weight, and described medium contains:
ZnSO
40.02-0.08%, (NH
4) SO
40.1～0.25%, NaCl 0.05～0.15%, NaHCO
30.05～0.1%, MgSO
40.02-0.08%, K
2HPO
40.02～0.1%, CH
3COONa0.1～0.2%, dregs of beans 0.005～0.1%, peptone 0.002～0.1%, surplus is a water;
At PH6.5-7.5, temperature 25-35 ℃, illumination 2500～6000Lux cultivated 120-300 hour down, obtained thick seed culture fluid;
(2) material that step (1) is obtained is inoculated in to produce and cultivates breeding in the jar, and medium is that benchmark contains with its weight:
Dregs of beans 0.005～0.1%, yeast extract 0.001～0.005%, peptone 0.001～0.005%, ZnSO
40.02-0.08%, MgCl
20.002～0.005%, NaCl0.04～0.1%, K
2HPO
40.01～0.05%, CaCO
30.05～0.15%, surplus is the molasses waste liquid;
Water blowback circulation oxygen, illumination 2500～6000Lux cultivated 100～250 hours, centrifugal filtration, the brown liquid that obtains clarifying, productive rate account for the 95-97% that this step is cultivated the material gross weight;
(3) add synergistic matter, synergistic matter is meant and can promotes class material plant growth, that strengthen anti-adversity ability that synergistic matter content is 0.1～2.0% of step (2) gained liquid weight; Mix the back direct packaging and become fluid product.
Synergistic matter of the present invention is one or more combinations in tea polysaccharide, sodium alginate, lentinan, xylo-oligosaccharide, shitosan, compound sodium nitrophenolate, study on brassinolide, the diethyl aminoethyl hexanoate.
Molasses waste liquid of the present invention is the molasses waste liquid of cane mill or Alcohol Plant's fermentation process, and it is as a kind of carrier of medium.This molasses waste liquid derives from the last molasses waste liquid in sugar refinery, and the main component of molasses is a carbohydrate, and cane molasses contains the about 24%-36% of sucrose, the about 12%-24% of other sugar; Molasses contain a small amount of thick protein, are generally 3%-6%, belong to the non protein nitrogen class more, and as ammonia, acid amides and nitrate etc., and amino-acid nitrogen only accounts for 38%-50%.。
Product of the present invention can be used as herbicides and safeners and weed killer herbicide is used; function with safety, synergy, degraded pedo relict chemical agent; after directly being applied to the crops of the various chemical pesticides of the normal use of crop growth period; can make the residual of agricultural chemicals such as miticides such as organophosphorus insecticide, chrysanthemum ester insecticide, Avermectin, dicofol and weed killer herbicide, bactericide; hang down 25～75% respectively than the residue of pesticide index that country limits; this is to improving quality of agricultural product, and protection environment and people's health plays an important role.For have more creation usefulness of tangible detoxifcation because of use of insecticide, bactericide, the improper poisoning that causes of hormone.In addition, can be used as a kind of volume increase agent, use separately, can improve crop yield.
Compare with prior art, the invention belongs to a kind of safener, antipoison, volume increase agent, fall residual dose, outstanding advantage is to have characteristics such as green, environmental protection, efficient, low cost, and it will produce significant impact to the raising of level of human health and the protection of ecotope.Detoxifcation of the present invention volume increase agent is widely applicable, and the effect brilliance has broken through the efficacy effect of single degrade residual, and it is fast to have a detoxifcation, and degradation is strong, and can regulate growth, alleviates poisoning, significantly improves features such as output.It is residual that the present invention not only can reduce medicine, mixes use with weed killer herbicide, insecticide, has safe, efficient, residual low, good characteristics such as poisoning is light, can be used as the chemistry security agent and especially be used with weed killer herbicide.
Embodiment
Following examples are used to illustrate the present invention.
Embodiment one:
(1) with the bacterial classification complexing agent, be that 1.2% of medium material gross weight is inoculated in the seed culture case and cultivates by the bacterial classification inoculation amount, medium is:
ZnSO
40.06%, (NH
4) SO
40.15%, NaCl 0.1%, NaHCO
30.08%, MgSO
40.05%, K
2HPO
40.08%, CH
3COONa 0.15%, dregs of beans 0.008%, peptone 0.009%;
At PH6.5-7.5, temperature 25-35 ℃, water blowback circulation oxygen, illumination 2500～6000Lux light was cultivated 200 hours.
(2) the resulting seed culture fluid of step (1) directly is inoculated in cultivation breeding in the production jar, medium is:
The molasses waste liquid of cane mill or Alcohol Plant's fermentation process is a carrier, dregs of beans 0.008%, yeast extract 0.0025%, peptone 0.003%, ZnSO
40.04%, MgCl
20.004%, NaCl0.07%, K
2HPO
40.04%, CaCO
30.08%.
Water blowback circulation gas adds illumination 5000～6000Lux light and cultivates more than 200 hours.
After cultivation in 200 hours, adopt centrifugation, the sepia viscous liquid that obtains clarifying accounts for 96% of this material gross weight.
(3) add synergistic matter, tea polysaccharide 0.08%, sodium alginate 0.3%, lentinan 0.1%, xylo-oligosaccharide 0.40%, compound sodium nitrophenolate 0.09%; Study on brassinolide 0.0004%, diethyl aminoethyl hexanoate 0.6% mixes.
Wherein, the culture fluid addition of step (2) gained is 98.43%, and 1.57%. directly mixes.
Finish the present invention after culture fluid added synergistic matter, direct packaging becomes fluid product.
Embodiment two
1, spawn culture and fermentation process step are with embodiment one
2, add synergistic matter, shitosan 0.4%, lentinan 0.1%, xylo-oligosaccharide 0.80%, diethyl aminoethyl hexanoate 0.6%.Surplus is the culture fluid of step (2) gained, accounts for 98.1%.Mix with synergistic matter and to get final product.
Finish the present invention after culture fluid added synergistic matter, direct packaging becomes fluid product.
Embodiment three
1, spawn culture and fermentation process step are with embodiment one
2, add synergistic matter, lentinan 0.1%, xylo-oligosaccharide 0.5%, compound sodium nitrophenolate 0.09%; Study on brassinolide 0.0006% mixes.Surplus is the culture fluid of step (2) gained, accounts for 99.31%, mixes with synergistic matter to get final product.
Finish the present invention after culture fluid added synergistic matter, direct packaging becomes fluid product.
Embodiment four
1, spawn culture and fermentation process step are with embodiment one
2, add synergistic matter, sodium alginate 0.6%, lentinan 0.2%, xylo-oligosaccharide 0.6%, compound sodium nitrophenolate 0.09%; Study on brassinolide 0.0006% mixes the culture fluid that surplus is step (a 2) gained, accounts for 98.51%, mixes with synergistic matter to get final product.
Finish the present invention after culture fluid added synergistic matter, direct packaging becomes fluid product.
Embodiment five:
Test method: the product that example 2 is obtained is applied to the soybean plant strain that sprayed multiple chemical pesticide and insecticide.Using method is that 50ml converts water 15kg, every mu of spraying liquid 45kg, and contrasting is to spray clear water., test area is 100 square metres of every processing, test period is behind the spraying pesticide 15 days, brings into use product of the present invention.
Test is divided into six groups of experiments promptly:
First group was used the malathion;
Second group was used dichlorvos;
The 3rd group was used sumicidin;
The 4th group was used dicofol;
The 5th group was used fomesafen;
The 6th group was used carbendazim
After soybean is gathered, detect its seed persticide residue through Zhejiang University, and with national GB2715-2005 " grain sanitary standard " Pesticide Residue index contrast, situation following (detection method: GB/T217332-1998):
| Title | Testing result | The Pesticide Residue index | Be lower than limit index |
| The malathion | 2mg/kg | ≤8mg/kg | 75％ |
| Dichlorvos | 0.06mg/kg | ≤0.1mg/kg | 40％ |
| Fenvalerate | 0.06mg/kg | ≤0.2mg/kg | 70％ |
| Dicofol | 0.08mg/kg | ≤0.1-0.2mg/kg | 20-60％ |
| Fomesafen | 0.035mg/kg | ≤0.1mg/kg | 65％ |
| Carbendazim | 0.095mg/kg | ≤0.2mg/kg | 52.5％ |
Embodiment six:
For 667 square metres of examination soybean areas, the experiment place is positioned at town Wu Putou village on May Day, has used weed killer herbicide beans sulphur grand before this crop sprays.At first, get the embodiment of the invention one product 50ml, convert 30 kilograms in water, evenly spray the face of blade and the leaf back side in soybean seedling, the spray clear water is contrast; Initial bloom stage is got this product 100ml, converts water and sprays once for 60 kilograms, evenly sprays blade face anyway, sprays twice altogether, and clear water is contrast.Other management conditions are with general land for growing field crops.Real the producing of results back meter.Output is 218.5 kilograms/667M after use this product
2, contrast is 185 kilograms/667M
2, 33.5 kilograms/667M of volume increase
2, amount of increase in production 18.8%.
Above embodiment is only for the present invention is further illustrated, and scope of the present invention is not subjected to the limitation of illustrated embodiment.
Claims (3)
1. photosynthetic bacteria detoxifcation volume increase agent is characterized in that:
Described detoxifcation volume increase agent is thick brown liquid, and this liquid contains photosynthetic bacteria and belongs to (Photosynthetic Bacteria, the microorganism complex of PSB-B4 PSB), PSB-1, three kinds of bacterial strains of PSB-4; The ratio of described three kinds of bacterial strains with when preparation pulvis weight count 1: 1: 1, effective viable bacteria content of photosynthetic bacteria is 500,000,000 a/gram in the described liquid, with described liquid weight is that synergistic matter content is 0.1 in this liquid of benchmark---2.0%, and described synergistic matter is meant a class material of the enhancing anti-adversity ability that can promote plant growth.
2. the production method of a kind of photosynthetic bacteria detoxifcation volume increase agent as claimed in claim 1 is characterized in that may further comprise the steps:
(1) described three kinds of bacterial strain pulvis being mixed with 1: 1: 1 part by weight, is that the 0.3-2% of medium material gross weight is inoculated in the seed culture case and cultivates by the bacterial classification inoculation amount, is benchmark with medium weight, and described medium contains:
ZnSO
40.02-0.08%, (NH4) SO
40.1～0.25%, NaCl 0.05～0.15%, NaHCO
30.05～0.1%, MgSO
40.02-0.08%, K
2HPO
40.02～0.1%, CH
3COONa0.1～0.2%, dregs of beans 0.005～0.1%, peptone 0.002～0.1%, surplus is a water;
At PH6.5-7.5, temperature 25-35 ℃, illumination 2500～6000Lux cultivated 120-300 hour down, obtained thick seed culture fluid;
(2) material that step (1) is obtained is inoculated in to produce and cultivates breeding in the jar, and medium is that benchmark contains with its weight:
Dregs of beans 0.005～0.1%, yeast extract 0.001～0.005%, peptone 0.001～0.005%, ZnSO
40.02-0.08%, MgCl
20.002～0.005%, NaCl0.04～0.1%, K
2HPO
40.01～0.05%, CaCO
30.05～0.15%, surplus is the molasses waste liquid;
Water blowback circulation oxygen, illumination 2500～6000Lux cultivated 100～250 hours, centrifugal filtration, the brown liquid that obtains clarifying, productive rate account for the 95-97% that this step is cultivated the material gross weight;
(3) add synergistic matter, synergistic matter is meant and can promotes class material plant growth, that strengthen anti-adversity ability that synergistic matter content is 0.1～2.0% of step (2) gained liquid weight; Mix the back direct packaging and become fluid product.
3. the production method of a kind of photosynthetic bacteria detoxifcation volume increase agent as claimed in claim 2 is characterized in that:
Described synergistic matter is one or more combinations in tea polysaccharide, sodium alginate, lentinan, xylo-oligosaccharide, shitosan, compound sodium nitrophenolate, study on brassinolide, the diethyl aminoethyl hexanoate.
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|---|---|---|---|---|
| CN102703413A (en) * | 2012-06-11 | 2012-10-03 | 沈阳大学 | Method for immobilizing photosynthetic bacteria by using mycelium pellets as biological carrier |
| CN102823594A (en) * | 2012-08-31 | 2012-12-19 | 广东植物龙生物技术有限公司 | Plant virus disease resistance composition comprising diethyl aminoethyl hexanoate and lentinan |
| CN109880779A (en) * | 2019-04-24 | 2019-06-14 | 安徽黄河水处理科技股份有限公司 | A kind of preparation method of photosynthetic bacteria liquid |
| CN110915520A (en) * | 2019-12-10 | 2020-03-27 | 安徽裕民生态农业有限公司 | Camellia oleifera seedling growing method |
| CN111499455A (en) * | 2020-04-23 | 2020-08-07 | 合水县振恺肥业有限公司 | Bio-organic fertilizer and preparation method and application thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102703413A (en) * | 2012-06-11 | 2012-10-03 | 沈阳大学 | Method for immobilizing photosynthetic bacteria by using mycelium pellets as biological carrier |
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| CN102823594A (en) * | 2012-08-31 | 2012-12-19 | 广东植物龙生物技术有限公司 | Plant virus disease resistance composition comprising diethyl aminoethyl hexanoate and lentinan |
| CN102823594B (en) * | 2012-08-31 | 2014-07-09 | 广东植物龙生物技术有限公司 | Plant virus disease resistance composition comprising diethyl aminoethyl hexanoate and lentinan |
| CN109880779A (en) * | 2019-04-24 | 2019-06-14 | 安徽黄河水处理科技股份有限公司 | A kind of preparation method of photosynthetic bacteria liquid |
| CN110915520A (en) * | 2019-12-10 | 2020-03-27 | 安徽裕民生态农业有限公司 | Camellia oleifera seedling growing method |
| CN111499455A (en) * | 2020-04-23 | 2020-08-07 | 合水县振恺肥业有限公司 | Bio-organic fertilizer and preparation method and application thereof |
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