CN109880779A - A kind of preparation method of photosynthetic bacteria liquid - Google Patents
A kind of preparation method of photosynthetic bacteria liquid Download PDFInfo
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- CN109880779A CN109880779A CN201910333075.4A CN201910333075A CN109880779A CN 109880779 A CN109880779 A CN 109880779A CN 201910333075 A CN201910333075 A CN 201910333075A CN 109880779 A CN109880779 A CN 109880779A
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Abstract
The invention discloses a kind of preparation methods of photosynthetic bacteria liquid, belong to bacterium solution preparation technical field.For photosynthetic bacteria higher cost is prepared in the prior art and the lower problem of photosynthetic bacteria obtained activity, the present invention provide a kind of preparation method of photosynthetic bacteria liquid, it includes the preparation of photosynthetic bacteria basic culture solution；The preparation of dregs of beans paste and it is inoculated with photosynthetic bacteria on the basic culture solution of preparation, and adds dregs of beans paste, basic culture solution is placed under illumination condition and is cultivated, after photosynthetic bacteria basic culture solution is in peony, dregs of beans paste is added, when photosynthetic bacteria is in thick shape, culture is completed.Raw material needed for the present invention prepares photosynthetic bacteria is less, avoids the cumbersome place of high cost and multiple compounds the production culture medium using yeast extract；Use dregs of beans for raw material, dregs of beans contains higher protein, can provide nutrition for the culture of photosynthetic bacteria, and cost is relatively low；Viable count superelevation in products obtained therefrom photosynthetic bacteria liquid is in thick shape as blood.
Description
Technical field
The invention belongs to bacterium solution preparation technical fields, more specifically to a kind of preparation method of photosynthetic bacteria liquid.
Background technique
The photosynthetic bacteria such as Rhodopseudomonas palustris, pod membrane of Rhodospirillaceae (Rhodospirillaceae) pseudomonas
Red pseudomonas, Rhodopseudomonas spheroides are the beneficial microbe being commonly used in aquatic products, environmental protection and feed, studies have shown that light
B family vitamin abundant, carotenoid, Co-Q10, folic acid etc., therefore, as dynamic can be generated by closing the metabolism of bacterium own growth
Object feed addictive has very high nutritive value.In addition, photosynthetic bacteria can effectively remove organic matter in waste water and
Nitrogen phosphate and sulfur compound is commonly used in aquaculture field to reduce the ammonia nitrogen in pool, nitrite and organic matter.Its culture medium
Formula also there are many kinds of, but comprehensive general view, for commercial product culture medium due to using more yeast extract, cost is relatively high, self-control
The growth concentration of culture medium bacterium is insufficient, and is less able to reach the bacterium number of mark in the bacterium solution of final output, usually light in mark
Combined bacteria bacterium number is 10~5,000,000,000 cfu (bacterium solution), 100~20,000,000,000 cfu (bacterium powder), but finds few can exceed that in finished product detection
The photosynthetic bacteria bacterium solution product of 2000000000 cfu, in commodity photosynthetic bacteria bacterium powder, also devitalization due to dry of most of viable bacteria.
It is also correspondingly improved regarding to the issue above, it is open such as Chinese Patent Application No. CN201310036820.1
Day the steps include: that (1) will be equipped with light which disclose a kind of method for preparing photosynthetic bacteria liquid on June 12nd, 2013
The cryopreservation tube for closing bacteria culture carries out dissolution recovery, and dissolved bacterium solution becomes recovery bacterium solution；(2) it on superclean bench, uses
Recovery bacterium solution is crossed on Solid media for plates, and scribed Solid media for plates is stood in 30 DEG C of constant incubators
After culture grows single colonie in 24~36 hours, with the single colonie on oese picking Solid media for plates on slant medium
Scribing line, scribed slant medium 24~36 hours bacterium colonies of stationary culture in 30 DEG C of incubators are grown；(3) bacterium colony is grown
Afterwards, the bacterium colony on slant medium is eluted as bacterium colony solution with photosynthetic bacteria liquid culture medium, bacterium colony solution carries out just
Secondary culture obtains cultivating bacterium solution for the first time, and first culture bacterium solution carries out switching culture and obtains switching culture bacterium solution.The present invention has training
The basigamy system of supporting and incubation are simple, and bacterial concentration is high, high-quality feature.The patent is disadvantageous in that: although preparation
Bacterial concentration higher cost that is high, but preparing, and error of crossing on culture medium is larger.
For another example Chinese Patent Application No. CN201210556654.3, publication date are on June 25th, 2014, which disclose
A kind of solution of photosynthetic bacteria consolidates the preparation method of double fermentation high concentration probiotics, including plating medium culture, test tube training
It supports, triangular flask culture, the preparation of secondary seed, the preparation of three-level seed, the preparation of finished product and etc..The shortcoming of the patent
It is: although processing cost is lower, is not easy to contaminate miscellaneous bacteria, preparation step is more, and efficiency is lower.
Summary of the invention
1. solving the problems, such as
For preparing photosynthetic bacteria higher cost and the lower problem of photosynthetic bacteria obtained activity, this hair in the prior art
It is bright that a kind of preparation method of photosynthetic bacteria liquid is provided.Raw material needed for the present invention prepares photosynthetic bacteria is less, avoids use
The cumbersome place of high cost and multiple compounds the production culture medium of yeast extract；Use dregs of beans for raw material, dregs of beans contains higher
Protein can provide nutrition for the culture of photosynthetic bacteria, and cost is relatively low；Viable count is super in products obtained therefrom photosynthetic bacteria liquid
Height is in thick shape as blood.
2. technical solution
To solve the above-mentioned problems, the technical solution adopted in the present invention is as follows:
A kind of preparation method of photosynthetic bacteria liquid, comprising the following steps:
(1) photosynthetic bacteria basic culture solution is prepared: including 2.0~2.5g/L of anhydrous sodium acetate, ammonium hydrogen carbonate 2.0~
2.5g/L, 0.3~0.5g/L of bitter salt, 0.5~1g/L of potassium dihydrogen phosphate；
(2) it prepares dregs of beans paste: dregs of beans being beaten into powder is added to the water and boiled, cool down；
(3) inoculated and cultured photosynthetic bacteria: the photosynthetic bacteria basic culture solution prepared is fitted into transparent culture vessel,
It is inoculated with photosynthetic bacteria strain in culture vessel, and adds dregs of beans paste, has all been added to photosynthetic bacteria strain and dregs of beans paste
Culture vessel is placed under illumination condition and cultivates by Cheng Hou, after photosynthetic bacteria basic culture solution is in peony, adds dregs of beans
Paste, when photosynthetic bacteria is in thick shape, culture is completed.
Further, dregs of beans beats powder and is sieved through 80 mesh holes in the step (2), the bean cake powder after sieve be added to the water into
Row is boiled, is cooled down.
Further, the bean cake powder after sieve is added to the water with the amount of 80~100g/L is boiled, is cooled down.
Further, the inoculum concentration of culture vessel inoculation photosynthetic bacteria is 12.5%~25% in the step (3).
Further, the additional amount of dregs of beans paste is 20ml.
Further, the liquid in culture vessel is not filled with the entire space in culture vessel, in culture vessel on
There are spaces in portion.
Further, the culture vessel is plastic bottle or culture dish.
Further, intensity of illumination is 2000~3000lux.
Further, dregs of beans paste was added every 3~4 days after photosynthetic bacteria basic culture solution is in peony.
3. beneficial effect
Compared with the prior art, the invention has the benefit that
(1) raw material needed for present invention preparation photosynthetic bacteria is less, avoids the photosynthetic bacteria culture fluid of business and uses yeast extract
High cost and Various Complex compound production culture medium cumbersome place；Use dregs of beans for one of raw material, dregs of beans contain compared with
High protein can provide nutrition for the culture of photosynthetic bacteria, and cost is relatively low；The preparation method step of whole photosynthetic bacteria
Relatively simple, operability is high, viable count superelevation in products obtained therefrom photosynthetic bacteria liquid, is in thick shape as blood；
(2) dregs of beans beat after powder and is sieved through 80 mesh holes by the present invention, is easy to be mixed into during dregs of beans carries out and beats powder
On the one hand the impurity of larger particles, the dregs of beans progress 80 mesh holes sieving after beating powder effectively prevent impurity and enter in bean cake powder
Influence the use of later period bean cake powder；On the other hand thinner dregs of beans powder can be obtained so that final obtained dregs of beans paste compared with
Be it is uniform, facilitate the use in later period；
(3) bean cake powder after screening is added to the water by the present invention with the amount of 80~100g/L is boiled, is cooled down；So that
The phenomenon that dregs of beans paste obtained is more uniform, is not susceptible to internal bond；Later period dregs of beans paste is facilitated to add into culture vessel
Add, so that the process of addition is more smooth；
(4) present invention in culture vessel internal upper part there are the space for accounting for entire culture vessel volume 3~8%, there are air,
Facilitate micro- aerobic photosynthetic bacteria to can be realized long period fast-growth, ensure that the close friend of photosynthetic bacterium growth environment, it is right
Facilitation is played in the growth of photosynthetic bacteria；
(5) intensity of illumination that the present invention takes is 2000~3000lux, the too low life for being unfavorable for photosynthetic bacteria of intensity of illumination
It is long, the excessively high quick diffusion for being unfavorable for photosynthetic bacteria of intensity of illumination, and too strong intensity of illumination may be such that photosynthetic bacteria sends out
Life and death is died；When intensity of illumination is between 2000~3000lux, so that the growth metabolism of photosynthetic bacteria is accelerated, promote photosynthetic bacteria
Growth；
(6) illumination that uses of the present invention is mainly sunshine on daytime, allow photosynthetic bacteria be suitable for daytime and night from
Right environment improves its adaptability to environment, is more advantageous to subsequent use, revert to the culture of photosynthetic bacteria naturally, most
Original state, can be effectively avoided in photosynthetic bacteria incubation it is possible that variation or degradation phenomena.
Detailed description of the invention
Fig. 1 is the process schematic of invention.
Specific embodiment
Nowadays, photosynthetic bacteria is the beneficial microbe being commonly used in aquatic products, environmental protection and feed, but its culture medium is matched
Due to using more yeast extract, cost is relatively high for side, and the growth concentration of self-made medium bacterium is insufficient, and final output
It is less able to reach the bacterium number of mark in bacterium solution, usually photosynthetic bacteria bacterium number is 10~5,000,000,000 cfu (bacterium solution) 100~200 in mark
Hundred million cfu (bacterium powder), but few photosynthetic bacteria bacterium solution products that can exceed that 2,000,000,000 cfu, commodity photosynthetic bacteria are found in finished product detection
In bacterium powder, also devitalization due to dry of most of viable bacteria.The continuous experiment of inventor's experience and analysis, provide a kind of light
Close the preparation method of bacterial solution.
As shown in Figure 1, a kind of preparation method of photosynthetic bacteria liquid, comprising the following steps:
(1) photosynthetic bacteria basic culture solution is prepared: including 2.0~2.5g/L of anhydrous sodium acetate, ammonium hydrogen carbonate 2.0~
2.5g/L, 0.3~0.5g/L of bitter salt, 0.5~1g/L of potassium dihydrogen phosphate；It is provided comprehensively for the culture of photosynthetic bacteria
Nutriment is basic, and photosynthetic bacteria basic culture solution described in the present embodiment is not necessarily to organic nitrogen source, and sodium acetate can effectively press down
The growth of miscellaneous bacteria processed, whole photosynthetic bacteria basic culture solution nutriment is comprehensive and environmental-friendly, and raw material is simple and cost is relatively low；
(2) it prepares dregs of beans paste: dregs of beans being beaten into powder is added to the water and boiled, cool down；Preferably, the dregs of beans beats powder warp
80 mesh holes are sieved, and the bean cake powder after sieve, which is added to the water, to be boiled, is cooled down；Be easy to be mixed into during dregs of beans carries out and beats powder compared with
The impurity of bulky grain, the dregs of beans after beating powder carries out the sieving of 80 mesh holes, and on the one hand effectively preventing impurity enters shadow in bean cake powder
Ring the use of later period bean cake powder；On the other hand thinner dregs of beans powder can be obtained, so that final dregs of beans paste obtained is more
Uniformly, facilitate the use in later period；Boiled in water if dregs of beans powder is relatively thick it is cooling after dregs of beans paste obtained be easy to happen it is viscous
Knot forms the use that bulk is unfavorable for later period dregs of beans paste；Further, by the bean cake powder after screening with 80~100g/L's
Amount, which is added to the water, is boiled, is cooled down；So that dregs of beans paste obtained is more uniform, the phenomenon that being not susceptible to internal bond；
Later period dregs of beans paste is facilitated to add into culture vessel, so that the process of addition is more smooth；
(3) inoculated and cultured photosynthetic bacteria: the photosynthetic bacteria basic culture solution prepared is fitted into transparent culture vessel,
Photosynthetic bacteria strain is inoculated in transparent culture vessel, the inoculum concentration of the photosynthetic bacteria strain is 12.5%~25%, and
Dregs of beans paste is added, the additional amount of the dregs of beans paste is 20ml, and excessive dregs of beans paste, which is added, will lead to photosynthetic bacteria consumption
Endless easy growth miscellaneous bacteria；The speed that very few dregs of beans paste will lead to photosynthetic bacteria growth is more slow, is unfavorable for photosynthetic thin
The culture of bacterium；After the completion of photosynthetic bacteria strain and dregs of beans paste are all added, by transparent culture vessel be placed under illumination condition into
Row culture；
The illumination condition is to use sunshine daytime, and night is irradiated using incandescent lamp or other light sources, and daytime is mainly day
According to allowing photosynthetic bacteria to be suitable for the natural environment at daytime and night, improve its adaptability to environment, be more advantageous to subsequent
It uses, so that the culture of photosynthetic bacteria is revert to the state naturally, most original, can be effectively avoided in photosynthetic bacteria incubation
It is possible that variation or degradation phenomena；For the intensity of illumination used for 2000~3000lux, intensity of illumination is too low to be unfavorable for light
The growth of bacterium, the excessively high quick diffusion for being unfavorable for photosynthetic bacteria of intensity of illumination are closed, and too strong intensity of illumination may make
It obtains photosynthetic bacteria and death occurs；When intensity of illumination is between 2000~3000lux, so that the growth metabolism of photosynthetic bacteria is accelerated,
Promote the growth of photosynthetic bacteria；After photosynthetic bacteria basic culture solution is in peony, dregs of beans paste 20ml was added every 3~4 days,
When carrying out adding dregs of beans paste, transparent culture vessel is not rocked, light is allowed to shine transparent culture vessel bottom as far as possible
Layer, until major part is all the photosynthetic bacteria flocculated bacteria of thick shape in entire culture vessel after adding 10 times or more, i.e., 30~40 days
Group, culture are completed；It is to constantly increase to the photosynthetic bacteria strain in culture vessel that the phase, which constantly adds dregs of beans paste, after incubation
Add nutrition material, so that photosynthetic bacteria is increased；
As shown in table 1,4 times for selecting 2 kinds of commodity photosynthetic bacteria bacterium solutions and method of the present invention being used to amplify are photosynthetic
In the bacterium number testing experiment of bacterium bacterium solution, by double-layer plate method of dilution butteron on plate, the bacterium number numerical value measured is as follows:
12 kinds of commodity photosynthetic bacterias of table and 4 Counting alive microbial results from training photosynthetic bacteria test bacteria liquid
It can be seen that by 1 measured value of table, actual measurement bacterium number can not show a candle to the numerical value in mark in commodity photosynthetic bacteria, and be significantly lower than
From training photosynthetic bacteria bacterium solution；Raw material needed for preparing photosynthetic bacteria using method of the present invention is less, and it is photosynthetic to avoid business
Bacteria culture fluid uses the high cost of yeast extract and the cumbersome place of the compound production culture medium of Various Complex；Use dregs of beans for original
One of material, dregs of beans contains higher protein, can provide nutrition for the culture of photosynthetic bacteria, cost is relatively low；It is whole photosynthetic thin
The preparation method step of bacterium is relatively simple, and operability is high, viable count superelevation in products obtained therefrom photosynthetic bacteria liquid, in as blood
Thick shape.
The present invention is further described below combined with specific embodiments below.
Embodiment 1
As shown in Figure 1, a kind of preparation method of photosynthetic bacteria liquid, comprising the following steps:
(1) photosynthetic bacteria basic culture solution is prepared: including anhydrous sodium acetate 2.0g/L, ammonium hydrogen carbonate 2.0g/L, seven hydrations
Magnesium sulfate 0.3g/L, potassium dihydrogen phosphate 0.5g/L；Comprehensive nutriment basis, the present embodiment are provided for the culture of photosynthetic bacteria
The photosynthetic bacteria basic culture solution is not necessarily to organic nitrogen source, and sodium acetate can effectively inhibit the growth of miscellaneous bacteria, whole photosynthetic
Bacterium basic culture solution nutriment is comprehensive and environmental-friendly, and raw material is simple and cost is relatively low；
(2) it prepares dregs of beans paste: dregs of beans being beaten into powder is added to the water and boiled, cool down；Preferably, the dregs of beans beats powder warp
80 mesh holes are sieved, and the bean cake powder after sieve, which is added to the water, to be boiled, is cooled down；Be easy to be mixed into during dregs of beans carries out and beats powder compared with
The impurity of bulky grain, the dregs of beans after beating powder carries out the sieving of 80 mesh holes, and on the one hand effectively preventing impurity enters shadow in bean cake powder
Ring the use of later period bean cake powder；On the other hand thinner dregs of beans powder can be obtained, so that final dregs of beans paste obtained is more
Uniformly, facilitate the use in later period；Boiled in water if dregs of beans powder is relatively thick it is cooling after dregs of beans paste obtained be easy to happen it is viscous
Knot forms the use that bulk is unfavorable for later period dregs of beans paste；Further, by the bean cake powder after screening with 80~100g/L's
Amount, which is added to the water, is boiled, is cooled down；So that dregs of beans paste obtained is more uniform, the phenomenon that being not susceptible to internal bond；
Later period dregs of beans paste is facilitated to add into culture vessel, so that the process of addition is more smooth；
(3) inoculated and cultured photosynthetic bacteria: the photosynthetic bacteria basic culture solution prepared is fitted into transparent culture vessel,
Preferably, the culture vessel is transparent plastic bottle, and materials are simple, reduces the toxigenic capacity of photosynthetic bacteria, is worthy to be popularized；Saturating
Photosynthetic bacteria strain is inoculated in bright plastic bottle, the inoculum concentration of the photosynthetic bacteria strain is 12.5%~25%, and adds dregs of beans
Paste, the additional amount of the dregs of beans paste are 20ml；After the completion of photosynthetic bacteria strain and dregs of beans paste are all added, by transparent modeling
Material bottle, which is placed under illumination condition, to be cultivated；Further, the liquid in transparent plastic bottle is not filled with entire inside plastic bottle
Space, in transparent plastic bottle internal upper part, there are the spaces for accounting for entire transparent plastic bottle volume 3~8%, and there are air, and it is micro- good to facilitate
The photosynthetic bacteria of oxygen can be realized long period fast-growth, ensure that the close friend of photosynthetic bacterium growth environment, to photosynthetic bacteria
Growth play facilitation；Other transparent containers can be used in the present invention；
The illumination condition is to use sunshine daytime, and night is irradiated using incandescent lamp or other light sources, and daytime is mainly day
According to allowing photosynthetic bacteria to be suitable for the natural environment at daytime and night, improve its adaptability to environment, be more advantageous to subsequent
It uses, so that the culture of photosynthetic bacteria is revert to the state naturally, most original, can be effectively avoided in photosynthetic bacteria incubation
It is possible that variation or degradation phenomena；For the intensity of illumination used for 2000~3000lux, intensity of illumination is too low to be unfavorable for light
The growth of bacterium, the excessively high quick diffusion for being unfavorable for photosynthetic bacteria of intensity of illumination are closed, and too strong intensity of illumination may make
It obtains photosynthetic bacteria and death occurs；When intensity of illumination is between 2000~3000lux, so that the growth metabolism of photosynthetic bacteria is accelerated,
Promote the growth of photosynthetic bacteria；After photosynthetic bacteria basic culture solution is in peony, dregs of beans paste 20ml was added every 3~4 days,
When carrying out adding dregs of beans paste, not rock transparent plastic bottle, make transparent plastic bottle upper layer keep transparency, allow light as far as possible
Shine transparent culture vessel bottom, it is most of in entire plastic bottle to be all until after adding 10 times or more, i.e., 30~40 days
The photosynthetic bacteria flocculation cenobium of thick shape, culture are completed.
Embodiment 2
Substantially with embodiment 1, a kind of preparation method of photosynthetic bacteria liquid, comprising the following steps:
(1) photosynthetic bacteria basic culture solution is prepared: including anhydrous sodium acetate 2.25g/L, ammonium hydrogen carbonate 2.25g/L, seven water
Close magnesium sulfate 0.4g/L, potassium dihydrogen phosphate 0.75g/L；Comprehensive nutriment basis, this reality are provided for the culture of photosynthetic bacteria
Photosynthetic bacteria basic culture solution described in example is applied without nitrogen, and sodium acetate can effectively inhibit the growth of miscellaneous bacteria, whole light
It is comprehensive and environmental-friendly to close bacterium basic culture solution nutriment, raw material is simple and cost is relatively low；
(2) it prepares dregs of beans paste: dregs of beans being beaten into powder is added to the water and boiled, cool down；Preferably, the dregs of beans beats powder warp
80 mesh holes are sieved, and the bean cake powder after sieve, which is added to the water, to be boiled, is cooled down；Be easy to be mixed into during dregs of beans carries out and beats powder compared with
The impurity of bulky grain, the dregs of beans after beating powder carries out the sieving of 80 mesh holes, and on the one hand effectively preventing impurity enters shadow in bean cake powder
Ring the use of later period bean cake powder；On the other hand thinner dregs of beans powder can be obtained, so that final dregs of beans paste obtained is more
Uniformly, facilitate the use in later period；Boiled in water if dregs of beans powder is relatively thick it is cooling after dregs of beans paste obtained be easy to happen it is viscous
Knot forms the use that bulk is unfavorable for later period dregs of beans paste；Further, the bean cake powder after screening is added with the amount of 80g/L
It boiled, cooled down into water；So that dregs of beans paste obtained is more uniform, the phenomenon that being not susceptible to internal bond；After convenient
Phase dregs of beans paste is added into culture vessel, so that the process of addition is more smooth；
(3) inoculated and cultured photosynthetic bacteria: the photosynthetic bacteria basic culture solution prepared is fitted into transparent culture vessel,
Preferably, the culture vessel is transparent plastic bottle, and materials are simple, reduces the toxigenic capacity of photosynthetic bacteria, is worthy to be popularized；Saturating
Photosynthetic bacteria strain is inoculated in bright plastic bottle, the inoculum concentration of the photosynthetic bacteria strain is 12.5%, and adds dregs of beans paste, institute
The additional amount for stating dregs of beans paste is 20ml；After the completion of photosynthetic bacteria strain and dregs of beans paste are all added, plastic bottle is placed in light
It is cultivated according under the conditions of；Further, the liquid in transparent plastic bottle is not filled with entire space inside plastic bottle, transparent
Plastic bottle internal upper part is there are the space for accounting for entire transparent plastic bottle volume 3~8%, there are air, facilitates micro- aerobic photosynthetic thin
Bacterium can be realized long period fast-growth, ensure that the close friend of photosynthetic bacterium growth environment, play to the growth of photosynthetic bacteria
Facilitation；Other transparent containers can be used in the present invention；
The illumination condition is to use sunshine daytime, and night is irradiated using incandescent lamp or other light sources, and daytime is mainly day
According to allowing photosynthetic bacteria to be suitable for the natural environment at daytime and night, improve its adaptability to environment, be more advantageous to subsequent
It uses, so that the culture of photosynthetic bacteria is revert to the state naturally, most original, can be effectively avoided in photosynthetic bacteria incubation
It is possible that variation or degradation phenomena；For the intensity of illumination used for 2000lux, intensity of illumination is too low to be unfavorable for photosynthetic bacteria
Growth, the excessively high quick diffusion for being unfavorable for photosynthetic bacteria of intensity of illumination, and too strong intensity of illumination may be such that it is photosynthetic
Bacterium occurs dead；When intensity of illumination is 2000lux, so that the growth metabolism of photosynthetic bacteria is accelerated, promote the growth of photosynthetic bacteria；
After photosynthetic bacteria basic culture solution is in peony, dregs of beans paste 20ml was added every 3 days, when carrying out adding dregs of beans paste,
Transparent plastic bottle is not rocked, light is allowed to shine transparent plastic bottle bottom as far as possible, until adding 10 times, i.e., 30 days
It afterwards, is all largely the photosynthetic bacteria flocculation cenobium of thick shape in entire plastic bottle, culture is completed.
Embodiment 3
Substantially with embodiment 1, a kind of preparation method of photosynthetic bacteria liquid, comprising the following steps:
(1) photosynthetic bacteria basic culture solution is prepared: including anhydrous sodium acetate 2.5g/L, ammonium hydrogen carbonate 2.5g/L, seven hydrations
Magnesium sulfate 0.5g/L, potassium dihydrogen phosphate 1g/L；Comprehensive nutriment basis, the present embodiment institute are provided for the culture of photosynthetic bacteria
The photosynthetic bacteria basic culture solution stated is not necessarily to nitrogen, and sodium acetate can effectively inhibit the growth of miscellaneous bacteria, whole photosynthetic bacteria
Basic culture solution nutriment is comprehensive and environmental-friendly, and raw material is simple and cost is relatively low；
(2) it prepares dregs of beans paste: dregs of beans being beaten into powder is added to the water and boiled, cool down；Preferably, the dregs of beans beats powder warp
80 mesh holes are sieved, and the bean cake powder after sieve, which is added to the water, to be boiled, is cooled down；Be easy to be mixed into during dregs of beans carries out and beats powder compared with
The impurity of bulky grain, the dregs of beans after beating powder carries out the sieving of 80 mesh holes, and on the one hand effectively preventing impurity enters shadow in bean cake powder
Ring the use of later period bean cake powder；On the other hand thinner dregs of beans powder can be obtained, so that final dregs of beans paste obtained is more
Uniformly, facilitate the use in later period；Boiled in water if dregs of beans powder is relatively thick it is cooling after dregs of beans paste obtained be easy to happen it is viscous
Knot forms the use that bulk is unfavorable for later period dregs of beans paste；Further, the bean cake powder after screening is added with the amount of 100g/L
Enter and is boiled, cooled down into water；So that dregs of beans paste obtained is more uniform, the phenomenon that being not susceptible to internal bond；It is convenient
Later period dregs of beans paste is added into culture vessel, so that the process of addition is more smooth；
(3) inoculated and cultured photosynthetic bacteria: the photosynthetic bacteria basic culture solution prepared is fitted into transparent culture vessel,
Preferably, the culture vessel is transparent plastic bottle, and materials are simple, is reduced while solving transparent plastic bottle pollution photosynthetic thin
The toxigenic capacity of bacterium, is worthy to be popularized；Middle inoculation photosynthetic bacteria strain, the inoculation of the photosynthetic bacteria strain in transparent plastic bottle
Amount is 25%, and adds dregs of beans paste, and the additional amount of the dregs of beans paste is 20ml；All to photosynthetic bacteria strain and dregs of beans paste
After the completion of addition, transparent plastic bottle is placed under illumination condition and is cultivated；Further, the liquid in transparent plastic bottle is not
Full of space entire inside plastic bottle, in transparent plastic bottle internal upper part, there are the skies for accounting for entire transparent plastic bottle volume 3~8%
Between, there are air, facilitate micro- aerobic photosynthetic bacteria to can be realized long period fast-growth, ensure that photosynthetic bacterium growth ring
The close friend in border plays facilitation to the growth of photosynthetic bacteria；Other transparent containers can be used in the present invention；
The illumination condition is to use sunshine daytime, and night is irradiated using incandescent lamp or other light sources, and daytime is mainly day
According to allowing photosynthetic bacteria to be suitable for the natural environment at daytime and night, improve its adaptability to environment, be more advantageous to subsequent
It uses, so that the culture of photosynthetic bacteria is revert to the state naturally, most original, can be effectively avoided in photosynthetic bacteria incubation
It is possible that variation or degradation phenomena；For the intensity of illumination used for 3000lux, intensity of illumination is too low to be unfavorable for photosynthetic bacteria
Growth, the excessively high quick diffusion for being unfavorable for photosynthetic bacteria of intensity of illumination, and too strong intensity of illumination may be such that it is photosynthetic
Bacterium occurs dead；When intensity of illumination is 3000lux, so that the growth metabolism of photosynthetic bacteria is accelerated, promote the growth of photosynthetic bacteria；
After photosynthetic bacteria basic culture solution is in peony, dregs of beans paste 20ml was added every 4 days, when carrying out adding dregs of beans paste,
Transparent plastic bottle is not rocked, light is allowed to shine transparent plastic bottle bottom as far as possible, until adding 10 times, i.e., 40 days
It afterwards, is all largely the photosynthetic bacteria flocculation cenobium of thick shape in entire plastic bottle, culture is completed.
Example of the present invention is only that preferred embodiments of the present invention will be described, not to present inventive concept and
Range is defined, and under the premise of not departing from design philosophy of the present invention, this field engineers and technicians are to technology of the invention
The various changes and improvements that scheme is made should all fall into protection scope of the present invention.
Claims (9)
1. a kind of preparation method of photosynthetic bacteria liquid, it is characterised in that: the following steps are included:
(1) photosynthetic bacteria basic culture solution is prepared: including 2.0~2.5g/L of anhydrous sodium acetate, 2.0~2.5g/L of ammonium hydrogen carbonate,
0.3~0.5g/L of bitter salt, 0.5~1g/L of potassium dihydrogen phosphate；
(2) it prepares dregs of beans paste: dregs of beans being beaten into powder is added to the water and boiled, cool down；
(3) inoculated and cultured photosynthetic bacteria: the photosynthetic bacteria basic culture solution prepared is fitted into transparent culture vessel, is being trained
It supports and is inoculated with photosynthetic bacteria strain in container, and add dregs of beans paste, after the completion of photosynthetic bacteria strain and dregs of beans paste are all added,
Culture vessel is placed under illumination condition and is cultivated, after photosynthetic bacteria basic culture solution is in peony, adds dregs of beans paste,
When photosynthetic bacteria is in thick shape, culture is completed.
2. a kind of preparation method of photosynthetic bacteria liquid according to claim 1, it is characterised in that: in the step (2)
Dregs of beans beats powder and is sieved through 80 mesh holes, and the bean cake powder after sieve, which is added to the water, to be boiled, is cooled down.
3. a kind of preparation method of photosynthetic bacteria liquid according to claim 2, it is characterised in that: bean cake powder after sieve with
The amount of 80~100g/L, which is added to the water, is boiled, is cooled down.
4. a kind of preparation method of photosynthetic bacteria liquid according to claim 1, it is characterised in that: in the step (3)
The inoculum concentration that culture vessel is inoculated with photosynthetic bacteria is 12.5%~25%.
5. a kind of preparation method of photosynthetic bacteria liquid according to claim 4, it is characterised in that: the addition of dregs of beans paste
Amount is 20ml.
6. a kind of preparation method of photosynthetic bacteria liquid according to claim 5, it is characterised in that: the liquid in culture vessel
Body is not filled with the entire space in culture vessel, and in culture vessel internal upper part, there are spaces.
7. a kind of preparation method of photosynthetic bacteria liquid according to claim 6, it is characterised in that: the culture vessel is
Plastic bottle or culture dish.
8. a kind of preparation method of photosynthetic bacteria liquid according to claim 7, it is characterised in that: intensity of illumination 2000
~3000lux.
9. a kind of preparation method of photosynthetic bacteria liquid according to claim 8, it is characterised in that: to photosynthetic bacteria basis
Culture solution added dregs of beans paste every 3~4 days in after peony.
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