CN109906842B - Application of hypersensitive protein complex enzyme preparation in preventing and treating huanglongbing - Google Patents

Application of hypersensitive protein complex enzyme preparation in preventing and treating huanglongbing Download PDF

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CN109906842B
CN109906842B CN201910194471.3A CN201910194471A CN109906842B CN 109906842 B CN109906842 B CN 109906842B CN 201910194471 A CN201910194471 A CN 201910194471A CN 109906842 B CN109906842 B CN 109906842B
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CN109906842A (en
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于凯波
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Yu Kaibo
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K1/00—Housing animals; Equipment therefor
    • A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
    • A01K1/0152—Litter
    • A01K1/0155—Litter comprising organic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005—Cultivation methods
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20—Cereals
    • A01G22/22—Rice
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K1/00—Housing animals; Equipment therefor
    • A01K1/015—Floor coverings, e.g. bedding-down sheets ; Stable floors
    • A01K1/0152—Litter
    • A01K1/0154—Litter comprising inorganic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/28—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture specially adapted for farming

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Abstract

The present application is a divisional application of 201811529411.4. The invention discloses an application of a hypersensitive protein complex enzyme preparation in preventing and treating huanglongbing, belonging to the technical field of enzyme preparations. The protein complex enzyme preparation is prepared by fermenting complex enzyme, zeolite powder, glucose and water; the compound enzyme consists of superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain. The protein complex enzyme preparation has strong biocatalytic decomposition effect, also has the effect of preventing and treating the huanglongbing, can solve the problem that the citrus is very easy to have the huanglongbing in the planting process, and is suitable for preventing and treating the citrus huanglongbing.

Description

Application of hypersensitive protein complex enzyme preparation in preventing and treating huanglongbing
The application is a divisional application with the application number of 201811529411.4, application date of 2018, 12 months and 13 days, and the invention name of 'a hypersensitive protein complex enzyme preparation and a preparation method and application thereof'.
Technical Field
The invention relates to an application of a hypersensitive protein complex enzyme preparation in preventing and treating huanglongbing, belonging to the technical field of enzyme preparations.
Background
The citrus greening disease is very easy to appear in the planting process of the citrus, and loss is brought to farmers, so that the prevention and control of the citrus greening disease are of great significance to citrus planting.
Disclosure of Invention
In order to solve the problem that citrus is easy to have yellow shoot, the invention provides the application of the hypersensitive protein complex enzyme preparation in preventing and treating the yellow shoot, and the adopted technical scheme is as follows:
the invention provides an application of a protein complex enzyme preparation in preventing and treating huanglongbing, in particular to an application in preventing and treating citrus huanglongbing, wherein the protein complex enzyme preparation is prepared by fermenting complex enzyme, zeolite powder, glucose and water; the compound enzyme consists of superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain.
Preferably, the complex enzyme, the zeolite powder, the glucose and the water are mixed according to the following parts by weight: 5.72 to 8.82 parts by weight of complex enzyme; 3.8 to 5.7 parts by weight of zeolite powder; 0.39 to 1.14 parts by weight of glucose; 7.6 to 11.4 parts by weight of water; wherein: the 5.72-8.82 parts by weight of complex enzyme is composed of 0.1-0.15 part by weight of superoxide dismutase, 1.5-2.5 parts by weight of bromelain, 2-3 parts by weight of cellulase, 1-1.5 parts by weight of lipase, 1-1.5 parts by weight of amylase, 0.05-0.07 part by weight of oligosaccharidase and 0.07-0.1 part by weight of papain.
More preferably, the complex enzyme, the zeolite powder, the glucose and the water are mixed according to the following parts by weight: 7.26 parts of compound enzyme; 4.2 parts of zeolite powder; 0.78 weight part of glucose; 9.4 parts by weight of water; wherein: the 7.26 parts by weight of complex enzyme consists of 0.12 part by weight of superoxide dismutase, 2 parts by weight of bromelain, 2.5 parts by weight of cellulase, 1.3 parts by weight of lipase, 1.2 parts by weight of amylase, 0.06 part by weight of oligosaccharidase and 0.08 part by weight of papain.
Preferably, the preparation method of the protein complex enzyme preparation comprises the steps of mixing the complex enzyme, the zeolite powder and water, adding oxygen, stirring uniformly, carrying out aerobic fermentation for 10-12 days at the temperature of 35-37 ℃ and under the condition that the pH value is 5.7, then adjusting the pH value to 6.5-6.8, adding glucose, continuing aerobic fermentation, then carrying out post-maturation culture for 45-50 days, standing after the culture is finished, and filtering to obtain the protein complex enzyme preparation.
More preferably, the number of days of aerobic fermentation is 10 days.
More preferably, the post maturation incubation is stirred every 15h during fermentation for 30min each time.
More preferably, the time of standing is 45 days.
The invention relates to a hypersensitive protein complex enzyme preparation which exists in a protein form and is not a microbial inoculum, the protein complex enzyme preparation has a strong biocatalytic decomposition effect, the negative reaction of harmful microorganisms in organisms is catalyzed and inhibited by catalyzing the positive reaction of beneficial microorganisms in the organisms, the balance of microecology is realized, the cell activity can be excited, heterologous proteins are rejected, the immunity of the organisms is greatly enhanced, the protein complex enzyme preparation has strong stability and strong anti-interference capability against foreign bacteria, is not damaged by foreign species, and has the characteristics of biological diversity and high catalytic reaction speed.
The invention has the beneficial effects that:
the invention provides a protein complex enzyme preparation which has strong biocatalytic decomposition effect. The protein complex enzyme preparation also has the effect of preventing and treating the huanglongbing.
Detailed Description
The present invention will be further described with reference to the following specific examples, but the present invention is not limited to these examples.
Example 1:
the embodiment provides a protein complex enzyme preparation, which is prepared by fermenting complex enzyme, zeolite powder, glucose and water; the compound enzyme consists of superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain; wherein:
5.72 parts of complex enzyme; 3.8 parts by weight of zeolite powder; 0.39 parts by weight of glucose; 7.6 parts by weight of water;
5.72 parts by weight of complex enzyme consists of 0.1 part by weight of superoxide dismutase, 1.5 parts by weight of bromelain, 2 parts by weight of cellulase, 1 part by weight of lipase, 1 part by weight of amylase, 0.05 part by weight of oligosaccharidase and 0.07 part by weight of papain.
The embodiment also provides a preparation method of the protein complex enzyme preparation, which comprises the steps of mixing superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase, papain, zeolite powder, glucose and water, then adding oxygen and stirring uniformly, carrying out aerobic fermentation for 10 days at the temperature of 35-37 ℃ and under the condition that the pH value is 5.7, then adding glucose to continue carrying out aerobic fermentation for 45 days, then adjusting the pH value to 6.5-6.8 to carry out post-maturation stage cultivation, stirring once every 15 hours during the fermentation period, stirring for 30min every time, standing for 45 days after the post-maturation stage cultivation is finished, and filtering to obtain the protein complex enzyme preparation.
Example 2
The embodiment provides a protein complex enzyme preparation, which is prepared by fermenting complex enzyme, zeolite powder, glucose and water; the compound enzyme consists of superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain; wherein:
8.82 parts of complex enzyme; 5.7 parts by weight of zeolite powder; 1.14 parts by weight of glucose; 11.4 parts by weight of water;
8.82 parts by weight of complex enzyme consists of 0.15 part by weight of superoxide dismutase, 2.5 parts by weight of bromelain, 3 parts by weight of cellulase, 1.5 parts by weight of lipase, 1.5 parts by weight of amylase, 0.07 part by weight of oligosaccharidase and 0.1 part by weight of papain.
The embodiment also provides a preparation method of the protein complex enzyme preparation, which comprises the steps of mixing superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase, papain, zeolite powder, glucose and water, then adding oxygen and stirring uniformly, carrying out aerobic fermentation for 10 days at the temperature of 35-37 ℃ and under the condition that the pH value is 5.7, then adding glucose to continue carrying out aerobic fermentation for 45 days, then adjusting the pH value to 6.5-6.8 to carry out post-maturation stage cultivation, stirring once every 15 hours during the fermentation period, stirring for 30min every time, standing for 45 days after the post-maturation stage cultivation is finished, and filtering to obtain the protein complex enzyme preparation.
Example 3
The embodiment provides a protein complex enzyme preparation, which is prepared by fermenting complex enzyme, zeolite powder, glucose and water; the compound enzyme consists of superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain; wherein:
7.26 parts of compound enzyme; 4.2 parts of zeolite powder; 0.78 weight part of glucose; 9.4 parts by weight of water;
7.26 parts by weight of complex enzyme consists of 0.12 part by weight of superoxide dismutase, 2 parts by weight of bromelain, 2.5 parts by weight of cellulase, 1.3 parts by weight of lipase, 1.2 parts by weight of amylase, 0.06 part by weight of oligosaccharidase and 0.08 part by weight of papain.
The embodiment also provides a preparation method of the protein complex enzyme preparation, which comprises the steps of mixing superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase, papain, zeolite powder, glucose and water, then adding oxygen and stirring uniformly, carrying out aerobic fermentation for 10 days at the temperature of 35-37 ℃ and under the condition that the pH value is 5.7, then adding glucose to continue carrying out aerobic fermentation for 45 days, then adjusting the pH value to 6.5-6.8 to carry out post-maturation stage cultivation, stirring once every 15 hours during the fermentation period, stirring for 30min every time, standing for 45 days after the post-maturation stage cultivation is finished, and filtering to obtain the protein complex enzyme preparation.
In order to verify the prevention and treatment effect of the protein complex enzyme on the salsa orange yellow dragon disease, experiments are carried out in apricot blossom town and Jiangkou town of Guangdong province, Mingkong county. Mixing the protein complex enzyme preparation in example 3 with water according to a mass ratio of 1:300 to prepare a protein complex enzyme solution, carrying out root cultivation on the citrus arenaria by using the protein complex enzyme solution prepared in example 3, observing the condition of hairy roots, and observing the condition of increasing organic matters for soil improvement. After the compound protease solution is sprayed on the leaf surfaces, the change of the survival rate of the psyllids is detected, the growth change and the tip control effect of the spring tips are observed, the change of channels and leaves is observed, the flowering quantity and the fruit setting rate are measured, and the detection, observation and evaluation work such as the condition that the branch leaves and the fruit development are normal is observed is carried out. The experimental acre is 9 mu.
And (3) test results: after the soil is improved, the organic matters of the soil are increased, the soil is soft, the air permeability is increased, harmful viruses are eliminated, the soil is recovered to be alive after three months, and a plurality of earthworms appear in the soil. The main root recovers the vitality and a large amount of hairy roots are visible. The branches and leaves grow, the leaves grow bigger and turn green, and the fruit setting rate is greatly improved. After the compound enzyme is used for one year, the fruit setting rate is improved, the fruit is full in autumn, the water content is sufficient, the sugar content is increased, and no yellow dragon disease virus is found after detection. The compound enzyme obtains favorable performance in the experimental process of treating the salose orange yellow dragon disease, and plants have clustered roots, luxuriant branches and leaves, quick recovery of fruit setting rate, full fruits, higher sugar content and better taste under the action of the compound enzyme and are praised by local fruit growers.
Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (2)

1. The application of the protein complex enzyme preparation in preventing and treating the huanglongbing is characterized in that the protein complex enzyme preparation is prepared by fermenting complex enzyme, zeolite powder, glucose and water; the compound enzyme consists of superoxide dismutase, bromelain, cellulase, lipase, amylase, oligosaccharidase and papain; the compound enzyme, the zeolite powder, the glucose and the water are mixed according to the following weight parts: 7.26 parts of compound enzyme; 4.2 parts of zeolite powder; 0.78 weight part of glucose; 9.4 parts by weight of water; wherein: the 7.26 parts by weight of complex enzyme consists of 0.12 part by weight of superoxide dismutase, 2 parts by weight of bromelain, 2.5 parts by weight of cellulase, 1.3 parts by weight of lipase, 1.2 parts by weight of amylase, 0.06 part by weight of oligosaccharidase and 0.08 part by weight of papain; the preparation method of the protein complex enzyme preparation comprises the steps of mixing the complex enzyme, zeolite powder and water, adding oxygen, stirring uniformly, carrying out aerobic fermentation for 10-12 days at the temperature of 35-37 ℃ and under the condition that the pH value is 5.7, adding glucose, continuing the aerobic fermentation, then carrying out post-maturation stage cultivation for 45-50 days, then adjusting the pH value to 6.5-6.8, standing after the cultivation is finished, and filtering to obtain the protein complex enzyme preparation; the post maturation stage cultivation is stirred once every 15h during fermentation, and each stirring is carried out for 30 min; the standing time was 45 days.
2. Use according to claim 1, wherein the number of days of aerobic fermentation is 10 days.
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CN109906842B (en) * 2018-12-13 2022-03-11 于凯波 Application of hypersensitive protein complex enzyme preparation in preventing and treating huanglongbing
CN110051828A (en) * 2019-04-23 2019-07-26 深圳前海千水生物科技有限公司 A kind of plant complex enzyme gynaecology detoxicating lotion
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