CN115708516B - Rooting agent for flower and seedling cuttage and preparation method and application thereof - Google Patents
Rooting agent for flower and seedling cuttage and preparation method and application thereof Download PDFInfo
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- 239000003795 chemical substances by application Substances 0.000 claims abstract description 54
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Abstract
The invention provides a rooting agent for flower and seedling cuttage, and a preparation method and application thereof. The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15-30 parts of indolebutyric acid, 10-20 parts of boric acid, 10-30 parts of naphthylacetic acid, 40-80 parts of salicylic acid, 3-5 parts of brassinolide, 20-30 parts of sugar, 20-30 parts of vitamin, 5-10 parts of inorganic salt, 10-20 parts of 6-benzyl aminopurine, 10-25 parts of traditional Chinese medicine compound extract, 15-20 parts of bitter lignin, 10-15 parts of myrcene and 50-80 parts of deionized water. The rooting agent for flower and seedling cuttage provided by the invention can effectively improve rooting quality and seedling rate, inhibit bacterial growth, reduce morbidity and improve survival rate, and is low in cost, high in rooting rate and survival rate, and free of environmental pollution.
Description
Technical Field
The invention relates to the field of flower and seedling propagation, in particular to a rooting agent for flower and seedling cuttage, and a preparation method and application thereof.
Background
Cutting is also called cutting, and is a common propagation method for cultivating plants, and the stems, leaves, roots, buds and the like of the plants (called cutting in gardening) can be cut, or the plants can be inserted into soil, sand or soaked in water, and the plants can be planted after rooting, so that the plants become independent new plants. In agriculture and forestry production, different plants have different requirements on conditions during cutting, and the requirements of the plants are known and matched, so that a higher breeding success rate can be obtained. The plant bar is very simple in the operation process of cutting propagation, and the cutting propagation needs low cost, consumes less time and the like, so the plant bar is the most common and most extensive asexual propagation method at present. The plant cutting propagation history is very long, and the salix psammophila Fan Mian is discussed as early as 'poem' but the initial cutting technology is only applicable to tree species which are easy to root and cut to survive. With the development of auxin analogues and exogenous rooting promoting agents, the rooting survival of plant cuttage is obviously promoted, and cuttage propagation of a plurality of tree species difficult to root is greatly successful.
The rooting agent is a growth regulator capable of promoting synthesis of endogenous hormones in plants and supplementing exogenous hormones, and can enhance the splitting capacity of roots and promote germination of adventitious root primordia by regulating and controlling important enzyme activities, so that root damage caused by cutting and transplanting measures is reduced, a seedling recovery period is shortened, the adaptability to the environment is improved, and the purposes of improving the healing rate of seedlings, survival rate, crop yield and quality and enhancing resistance are achieved. At present, rooting agents are widely applied to the propagation of the seed-inserted seedlings of the bar, and are also widely applied to field crops.
Chinese patent No. 10462832B discloses a cutting propagation method of ancient arborvitae, which comprises sequentially treating cutting slips derived from ancient arborvitae with potassium permanganate, sodium lignin and hormone to obtain treated cutting slips, cutting and cultivating the treated cutting slips in a matrix to realize cutting propagation of ancient arborvitae, shortening rooting time of ancient arborvitae, and simultaneously treatingAccording to the method, 600-4000mg/L of naphthalene acetic acid and indolebutyric acid are used as hormone to treat the cutting slips, the concentration of the naphthalene acetic acid and indolebutyric acid is greatly higher than the proper concentration, the high-concentration auxin can interfere the normal protein and nucleic acid metabolic process of the cutting slips, so that the growth of organisms is slowed down and even stopped, dead seedlings can be caused, the auxin can be photo-oxidized and decomposed, the cutting slips are suddenly contacted with the auxin with high concentration in the initial stage, the organisms grow slowly, and no auxin is available in the later stage, so that the cutting survival rate of the method is not high. The patent with application number 201210001504.6 discloses a combined rooting agent of gamma-polyglutamic acid and naphthylacetic acid and a use method thereof, wherein the rooting agent contains pure biological fermentation active ingredients of gamma-polyglutamic acid, naphthylacetic acid, indolebutyric acid and MgSO 4 、MnSO 4 、HBO 3 Na and Na 2 MoO 4 The rooting agent has high activity biological function, can accelerate plant root cell division speed, promote rooting, strengthen root and improve the absorption capacity of root system to nutrition, is suitable for transplanting vegetable seedlings such as tomatoes, green vegetables, cucumbers, peppers, rapes and the like, cutting or transplanting woody trees and flowers with difficult rooting, but has slower rooting speed, can not meet the requirements of practical application, and has the survival rate to be further improved.
Therefore, in order to meet the demands of practical application, it is necessary to provide a rooting agent for flowers and seedlings cutting, which has the advantages of low cost, high rooting rate, high survival rate and no pollution.
Disclosure of Invention
Aiming at the problems in the prior art, the invention aims to provide the rooting agent for flowers and seedlings cutting, which has the advantages of low cost, high rooting rate, high survival rate and no pollution, so as to meet the requirements of practical application and environmental protection.
To achieve the purpose, the technical scheme of the invention is as follows:
the invention provides a rooting agent for flower and seedling cuttage, which comprises the following raw materials in parts by weight: 15-30 parts of indolebutyric acid, 10-20 parts of boric acid, 10-30 parts of naphthylacetic acid, 40-80 parts of salicylic acid, 3-5 parts of brassinolide, 20-30 parts of sugar, 20-30 parts of vitamin, 5-10 parts of inorganic salt, 10-20 parts of 6-benzyl aminopurine, 10-25 parts of traditional Chinese medicine compound extract, 15-20 parts of bitter lignin, 10-15 parts of myrcene and 50-80 parts of deionized water.
In some embodiments of the invention, the sugar is selected from one or more of sucrose, oligosaccharides, stevioside.
In some embodiments of the invention, the vitamin is selected from one or more of vitamin B, vitamin C, vitamin H.
In some embodiments of the invention, the vitamin B is selected from vitamin B 2 Vitamin B 6 Vitamin B 12 One or more combinations of the above).
In some embodiments of the invention, the inorganic salt is selected from sodium nitrate or sodium dihydrogen phosphate.
In some embodiments of the present invention, the compound extract of traditional Chinese medicine is a mixed solution of hibiscus extract, red sage extract and salsola collina extract, wherein the volume ratio of hibiscus extract, red sage extract and salsola collina extract is 2-3:1-2:1 (for example, can be 2:1:1, 2:1.5:1, 2:2:1, 3:1:1, 3:1.5:1, 3:2:1 or any value thereof).
In some embodiments of the present invention, the preparation method of the hibiscus syriacus/red sage/salsola collina extract comprises the following steps:
(1) Pulverizing Hibiscus syriacus/Saviae Miltiorrhizae radix/herba Salsolae Collinae to obtain Hibiscus syriacus/Saviae Miltiorrhizae radix/herba Salsolae Collinae powder;
(2) Adding white sugar and hibiscus syriacus/red sage root/salsola collina powder into water, and stirring and mixing uniformly to obtain a fermentation base material;
(3) And (3) adding probiotics into the fermentation base material obtained in the step (2) for sealing and fermenting at constant temperature, collecting fermentation products for solid-liquid separation, and collecting filtrate to obtain hibiscus syriacus/red sage root/swamp cabbage extract.
In the preparation method of the hibiscus syriacus/red sage/salsola collina extract of the present invention, the mass ratio of the hibiscus syriacus/red sage/salsola collina powder, white sugar and water in the step (2) is 10-20:5-10:100 (for example, may be 10:5:100, 11:6:100, 12:7:100, 13:8:100, 14:9:100, 15:5:100, 16:6:100, 17:7:100, 18:8:100, 19:9:100, 20:10:100 or any value thereof).
In the preparation method of the hibiscus syriacus/red sage root/salsola collina extract, the probiotics in the step (3) comprise one or more of lactobacillus plantarum, lactobacillus bulgaricus, streptococcus thermophilus and lactobacillus acidophilus; the fermentation substrate inoculum size is 0.5% -1% (e.g. may be 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1% or any value therein); the sealed constant temperature fermentation temperature is 28-35deg.C (for example, 28deg.C, 29 deg.C, 30deg.C, 31deg.C, 32deg.C, 33deg.C, 35deg.C or any value thereof), and the fermentation time is 15-20 days (for example, 15 days, 16 days, 17 days, 18 days, 19 days, 20 days or any value thereof).
The preparation method of the rooting agent for flower and seedling cuttage comprises the following steps:
(1) Adding salicylic acid, indolebutyric acid, naphthylacetic acid and boric acid into deionized water, and fully stirring for standby;
(2) Adding brassinolide, sugar, vitamins, inorganic salt, 6-benzyl amino purine and bitter lignin into the solution obtained in the step (1), and stirring and fully mixing;
(3) Adding the traditional Chinese medicine compound extract and myrcene into the solution obtained in the step (2), and continuously stirring to obtain a product, namely the rooting agent.
The application of the rooting agent for flower and seedling cuttage in the field of flower and seedling propagation, and the application method in practical application comprises the following steps:
(1) Selecting current annual tillering shoots or current annual semi-lignified branches from plants which grow robustly and have no diseases and insect pests as cuttage branches for later use;
(2) Soaking the base part of the cuttage branch obtained in the step (1) in the rooting agent to obtain a soaked branch;
(3) Inserting the soaked branches obtained in the step (2) into a cutting matrix, culturing, transplanting after culturing, and continuously spraying rooting agent stock solution for 1-2 times within 2 weeks after transplanting.
In some embodiments of the invention, the length of the cutting shoots is 5-15 cm (e.g., can be 5cm, 6cm, 7cm, 8cm, 9cm, 10cm, 11cm, 12cm, 13cm, 14cm, 15cm, or any value therein), and 3-5 (e.g., can be 3, 4, or 5) leaves are retained at the top of each cutting shoot.
In some embodiments of the invention, the culturing period is 2-3 weeks (e.g., may be 2 weeks, 3 weeks, or any value therein), the culturing environment temperature is 20-25 ℃ (e.g., may be 20 ℃, 21 ℃, 22 ℃, 23 ℃, 24 ℃, 25 ℃, or any value therein), the culturing environment relative humidity is 65% -85% (e.g., may be 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, or any value therein), flowers and seedlings are sprayed with the rooting agent once every week during culturing, and the spraying period is in the morning or evening with a concentration of 30% -40% (e.g., may be 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, or any value therein).
In some embodiments of the invention, the cutting substrate is peatmoss, and the transplanting substrate is selected from one or more of loess, peatmoss, diatomaceous earth.
Compared with the prior art, the invention has the following beneficial effects:
(1) The rooting agent for flower and seedling cuttage provided by the invention is used by matching salicylic acid with boric acid, can effectively promote the formation of lateral root primordia, improve the rooting quality and seedling rate, improve the root activity, promote the absorption and utilization of nutrient substances, can regulate the absorption and utilization of nutrient substances by flowers and seedlings in adverse circumstances, and can provide nutrient substances to promote root growth by being supplemented with sugar, vitamins and inorganic salts in the formula, thereby having better rooting rate.
(2) The rooting agent for flower and seedling cuttage provided by the invention is prepared by mixing the hibiscus extract, the red sage extract and the swamp cabbage extract, and can effectively inhibit bacterial growth, reduce morbidity and promote survival rate by inhibiting bacterial hypha growth and spore germination.
(3) According to the rooting agent for flower and seedling cuttage, the added myrcene plays a role in resisting bacteria and viruses under the synergistic effect of the myrcene and the traditional Chinese medicine compound extract, so that root hair cell division is promoted, and the survival rate of flowers and seedlings is improved.
(4) The rooting agent for flower and seedling cuttage provided by the invention has the advantages of simple preparation method, low cost and no chemical pollution to the environment.
Detailed Description
The invention will be described below in connection with specific embodiments. The following examples are illustrative of the present invention and are not intended to limit the present invention. Other combinations and various modifications within the spirit of the invention may be made without departing from the spirit or scope of the invention. In the examples below, the compounds and related reagents used are commercially available.
Preparation of extract of hibiscus syriacus
The preparation method comprises the following steps:
(1) Pulverizing Hibiscus syriacus to obtain Hibiscus syriacus powder;
(2) Adding 10g of white sugar and 150g of hibiscus powder into 1kg of water, and stirring and uniformly mixing to obtain a fermentation base material;
(3) Adding lactobacillus bulgaricus accounting for 0.5 percent of the mass of the fermentation base material obtained in the step (2), sealing and fermenting for 15 days at the temperature of 28 ℃, collecting fermentation products, carrying out solid-liquid separation, and collecting filtrate to obtain the hibiscus syriacus extract.
Preparation of red sage root extract
The preparation method comprises the following steps:
(1) Pulverizing Saviae Miltiorrhizae radix to obtain Saviae Miltiorrhizae radix powder;
(2) Adding 10g of white sugar and 150g of red sage root powder into 1kg of water, and stirring and uniformly mixing to obtain a fermentation base material;
(3) Adding lactobacillus bulgaricus accounting for 0.5 percent of the mass of the fermentation base material obtained in the step (2), sealing and fermenting for 15 days at the temperature of 28 ℃, collecting fermentation products, carrying out solid-liquid separation, and collecting filtrate to obtain the red sage root extract.
Preparation of herba Salsolae Collinae extract
The preparation method comprises the following steps:
(1) Pulverizing herba Salsolae Collinae to obtain herba Salsolae Collinae powder;
(2) Adding 10g of white sugar and 150g of salsola collina powder into 1kg of water, and stirring and uniformly mixing to obtain a fermentation base material;
(3) Adding lactobacillus bulgaricus accounting for 0.5 percent of the mass of the fermentation base material obtained in the step (2), sealing and fermenting for 15 days at the temperature of 28 ℃, collecting fermentation products, performing solid-liquid separation, and collecting filtrate to obtain the herba Salsolae Collinae extract.
Example 1
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 30 parts of indolebutyric acid, 20 parts of boric acid, 30 parts of naphthylacetic acid, 80 parts of salicylic acid, 5 parts of brassinolide, 30 parts of sucrose and vitamin B 2 30 parts of sodium nitrate 10 parts, 20 parts of 6-benzyl aminopurine, 25 parts of a traditional Chinese medicine compound extract (the volume ratio of hibiscus extract, red sage extract and salsola collina extract is 3:2:1), 20 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water.
The preparation method comprises the following steps:
(1) Adding salicylic acid, indolebutyric acid, naphthylacetic acid and boric acid into deionized water, and fully stirring for standby;
(2) Adding brassinolide, sucrose and vitamin B into the solution obtained in the step (1) 2 Sodium nitrate, 6-benzyl amino purine and bitter lignin are stirred and fully mixed;
(3) And (3) adding the traditional Chinese medicine composite extract and myrcene into the solution obtained in the step (2), and continuously stirring to obtain the rooting agent for flower and seedling cuttage.
Example 2
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 20 parts of boric acid, 30 parts of naphthylacetic acid, 80 parts of salicylic acid, 5 parts of brassinolide, 30 parts of sucrose and vitaminsB 2 30 parts of sodium nitrate 10 parts, 20 parts of 6-benzyl aminopurine, 25 parts of a traditional Chinese medicine compound extract (the volume ratio of hibiscus extract, red sage extract and salsola collina extract is 3:2:1), 20 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water.
The preparation method is the same as in example 1.
Example 3
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 10 parts of boric acid, 30 parts of naphthylacetic acid, 80 parts of salicylic acid, 5 parts of brassinolide, 30 parts of sucrose and vitamin B 2 30 parts of sodium nitrate 10 parts, 20 parts of 6-benzyl aminopurine, 25 parts of a traditional Chinese medicine compound extract (the volume ratio of hibiscus extract, red sage extract and salsola collina extract is 3:2:1), 20 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water.
Example 4
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 10 parts of boric acid, 10 parts of naphthylacetic acid, 80 parts of salicylic acid, 5 parts of brassinolide, 30 parts of sucrose and vitamin B 2 30 parts of sodium nitrate 10 parts, 20 parts of 6-benzyl aminopurine, 25 parts of a traditional Chinese medicine compound extract (the volume ratio of hibiscus extract, red sage extract and salsola collina extract is 3:2:1), 20 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water.
The preparation method is the same as in example 1.
Example 5
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 10 parts of boric acid, 10 parts of naphthylacetic acid, 40 parts of salicylic acid, 5 parts of brassinolide, 30 parts of sucrose and vitamin B 2 30 parts of sodium nitrate 10 parts, 20 parts of 6-benzyl aminopurine, 25 parts of a traditional Chinese medicine compound extract (the volume ratio of hibiscus extract, red sage extract and salsola collina extract is 3:2:1), 20 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water.
The preparation method is the same as in example 1.
Example 6
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 10 parts of boric acid, 10 parts of naphthylacetic acid, 40 parts of salicylic acid, 3 parts of brassinolide, 30 parts of sucrose and vitamin B 2 30 parts of sodium nitrate 10 parts, 20 parts of 6-benzyl aminopurine, 25 parts of a traditional Chinese medicine compound extract (the volume ratio of hibiscus extract, red sage extract and salsola collina extract is 3:2:1), 20 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water.
The preparation method is the same as in example 1.
Example 7
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 10 parts of boric acid, 10 parts of naphthylacetic acid, 40 parts of salicylic acid, 3 parts of brassinolide, 20 parts of sucrose and vitamin B 2 30 parts of sodium nitrate 10 parts, 20 parts of 6-benzyl aminopurine, 25 parts of a traditional Chinese medicine compound extract (the volume ratio of hibiscus extract, red sage extract and salsola collina extract is 3:2:1), 20 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water.
The preparation method is the same as in example 1.
Example 8
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 10 parts of boric acid, 10 parts of naphthylacetic acid, 40 parts of salicylic acid, 3 parts of brassinolide, 20 parts of sucrose and vitamin B 2 20 parts of sodium nitrate 10 parts, 20 parts of 6-benzyl aminopurine, 25 parts of a traditional Chinese medicine compound extract (the volume ratio of hibiscus extract, red sage extract and salsola collina extract is 3:2:1), 20 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water.
The preparation method is the same as in example 1.
Example 9
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 10 parts of boric acid, 10 parts of naphthylacetic acid,40 parts of salicylic acid, 3 parts of brassinolide, 20 parts of sucrose and vitamin B 2 20 parts of sodium nitrate 5 parts, 20 parts of 6-benzyl aminopurine, 25 parts of a traditional Chinese medicine compound extract (the volume ratio of hibiscus extract, red sage extract and salsola collina extract is 3:2:1), 20 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water.
The preparation method is the same as in example 1.
Example 10
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 10 parts of boric acid, 10 parts of naphthylacetic acid, 40 parts of salicylic acid, 3 parts of brassinolide, 20 parts of sucrose and vitamin B 2 20 parts of sodium nitrate 5 parts, 10 parts of 6-benzyl aminopurine, 25 parts of a traditional Chinese medicine compound extract (the volume ratio of a hibiscus extract, a red sage extract and a salsola collina extract is 3:2:1), 20 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water.
The preparation method is the same as in example 1.
Example 11
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 10 parts of boric acid, 10 parts of naphthylacetic acid, 40 parts of salicylic acid, 3 parts of brassinolide, 20 parts of sucrose and vitamin B 2 20 parts of sodium nitrate 5 parts, 10 parts of 6-benzyl aminopurine, 10 parts of a traditional Chinese medicine compound extract (the volume ratio of a hibiscus extract, a red sage extract and a salsola collina extract is 3:2:1), 20 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water.
The preparation method is the same as in example 1.
Example 12
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 10 parts of boric acid, 10 parts of naphthylacetic acid, 40 parts of salicylic acid, 3 parts of brassinolide, 20 parts of sucrose and vitamin B 2 20 parts of sodium nitrate 5 parts, 10 parts of 6-benzyl aminopurine, 10 parts of traditional Chinese medicine compound extract (the volume ratio of hibiscus syriacus extract, red sage extract and sallow greenbrier extract is 2:1:1)20 parts of element, 15 parts of myrcene and 80 parts of deionized water.
The preparation method is the same as in example 1.
Example 13
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 10 parts of boric acid, 10 parts of naphthylacetic acid, 40 parts of salicylic acid, 3 parts of brassinolide, 20 parts of sucrose and vitamin B 2 20 parts of sodium nitrate 5 parts, 10 parts of 6-benzyl aminopurine, 10 parts of a traditional Chinese medicine compound extract (the volume ratio of a hibiscus extract, a red sage extract and a salsola collina extract is 2:1:1), 15 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water.
The preparation method is the same as in example 1.
Example 14
The rooting agent for flower and seedling cuttage comprises the following raw materials in parts by weight: 15 parts of indolebutyric acid, 10 parts of boric acid, 10 parts of naphthylacetic acid, 40 parts of salicylic acid, 3 parts of brassinolide, 20 parts of sucrose and vitamin B 2 20 parts of sodium nitrate 5 parts, 10 parts of 6-benzyl aminopurine, 10 parts of a traditional Chinese medicine compound extract (the volume ratio of a hibiscus extract, a red sage extract and a salsola collina extract is 2:1:1), 15 parts of kudzein, 10 parts of myrcene and 80 parts of deionized water.
The preparation method is the same as in example 1.
Comparative example 1
The difference from example 1 is that: salicylic acid and boric acid are not added.
Comparative example 2
The difference from example 1 is that: salicylic acid is not added.
Comparative example 3
The difference from example 1 is that: boric acid is not added.
Comparative example 4
The difference from example 1 is that: the traditional Chinese medicine compound extract is not added.
Comparative example 5
The difference from example 1 is that: no myrcene was added.
Comparative example 6
The difference from example 1 is that: the traditional Chinese medicine compound extract and myrcene are not added.
Selecting the current annual branch of a healthy and strong improved variety of camellia oleifera as a cutting seedling, wherein the length of the cutting branch is 10cm, 3 leaves are reserved at the top of the branch, transplanting the branch into loess after cultivating the branch in peatmoss for 3 weeks, the cultivation temperature is 25 ℃, the relative humidity is 70%, and the rooting agent for flower and seedling cutting prepared by each example and comparative example with the concentration of 40% is used for the cutting seedling during the cultivation, and the rooting agent stock solution is sprayed twice on the 7 th day and the 14 th day after transplanting, and the performance test is carried out by sampling on the 20 th day, wherein the test method comprises the following steps:
(1) Lateral root primordium: measuring by using a Feulgen staining method;
(2) Root system activity: reduction strength by tetrazole (μg.g FW) measured by TTC method -1 ·h -1 ) To represent root system activity;
(3) Survival = survival number/total number of plants planted 100%;
(4) Rotten root rate = 100% of total number of plants/plants with rotten root.
The measurement results are shown in Table 1:
TABLE 1 rooting agent Performance test results for example, comparative example flowers and seedlings
As can be seen from comparison of the test results of comparative examples 1, 2 and 3 and example 1, the rooting agent for cutting flowers and seedlings prepared in example 1 effectively improves the root activity and the lateral root primordial density of cutting seedlings by adding salicylic acid and boric acid; as can be seen from the comparison of the test results of comparative example 4 and example 1, by adding the Chinese medicinal compound extract, the growth of hypha and spore germination of pathogenic bacteria can be effectively inhibited, and the disease rate is reduced; as is clear from comparison of comparative examples 5 and 6 with example 1, the rotten root rate can be significantly reduced by adding the Chinese medicinal compound extract and myrcene. As can be seen from comprehensive comparison of test results of each example and comparative examples, the rooting agent for flower and seedling cuttage provided by the invention effectively improves rooting quality and seedling rate by adding salicylic acid and boric acid, and meanwhile, by adding the traditional Chinese medicine compound extract and myrcene, bacterial growth can be effectively inhibited, morbidity is reduced, and survival rate is improved.
The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the present invention and to implement it, but not limit the scope of the present invention, and all equivalent changes or modifications made according to the spirit of the present invention should be included in the scope of the present invention.
Claims (6)
1. The rooting agent for flower and seedling cuttage is characterized by comprising the following raw materials in parts by weight: 30 parts of indolebutyric acid, 20 parts of boric acid, 30 parts of naphthylacetic acid, 80 parts of salicylic acid, 5 parts of brassinolide, 30 parts of sucrose and vitamin B 2 30 parts of sodium nitrate 10 parts, 20 parts of 6-benzyl aminopurine, 25 parts of a traditional Chinese medicine compound extract, 20 parts of kudzein, 15 parts of myrcene and 80 parts of deionized water;
the traditional Chinese medicine compound extract is a mixed solution of hibiscus extract, red sage extract and salsola collina extract, and the volume ratio of the hibiscus extract to the red sage extract to the salsola collina extract is 3:1;
the preparation method of the hibiscus syriacus/red sage root/swamp cabbage extracting solution comprises the following steps:
(1) Pulverizing Hibiscus syriacus/Saviae Miltiorrhizae radix/herba Salsolae Collinae to obtain Hibiscus syriacus/Saviae Miltiorrhizae radix/herba Salsolae Collinae powder;
(2) Adding white sugar and hibiscus syriacus/red sage root/salsola collina powder into water, and stirring and mixing uniformly to obtain a fermentation base material;
(3) Adding probiotics into the fermentation base material obtained in the step (2) for sealing and constant-temperature fermentation, collecting fermentation products for solid-liquid separation, and collecting filtrate to obtain hibiscus syriacus/red sage root/swamp cabbage extract;
in the step (2), the mass ratio of the hibiscus syriacus/red sage root/salsola collina powder to the white sugar to the water is 15:10:100; the probiotics in the step (3) are lactobacillus bulgaricus; the inoculation amount of the fermentation substrate is 0.5%; the sealed constant-temperature fermentation temperature is 28 ℃, and the fermentation time is 15 days.
2. A method for preparing the rooting agent for flower and seedling cuttage according to claim 1, which comprises the following steps:
(1) Adding salicylic acid, indolebutyric acid, naphthylacetic acid and boric acid into deionized water, and fully stirring for standby;
(2) Adding brassinolide, sucrose and vitamin B into the solution obtained in the step (1) 2 Sodium nitrate, 6-benzyl amino purine and bitter lignin are stirred and fully mixed;
(3) Adding the traditional Chinese medicine compound extract and myrcene into the solution obtained in the step (2), and continuously stirring to obtain a product, namely the rooting agent.
3. The application of the rooting agent for flowers and seedlings cutting according to claim 1 in the field of flowers and seedlings reproduction, wherein the application method comprises the following steps:
(1) Selecting current annual tillering shoots or current annual semi-lignified branches from plants which grow robustly and have no diseases and insect pests as cuttage branches for later use;
(2) Soaking the base part of the cuttage branch obtained in the step (1) in the rooting agent to obtain a soaked branch;
(3) Inserting the soaked branches obtained in the step (2) into a cutting matrix, culturing, transplanting after culturing, and continuously spraying rooting agent stock solution for 1-2 times within 2 weeks after transplanting.
4. The rooting agent for flowers and seedlings cutting according to claim 3, wherein the length of the cutting branches is 5-15 cm, and 3-5 leaves are reserved at the top of each cutting branch.
5. The rooting agent for flower and seedling cuttage according to claim 3, wherein the cultivation time is 2-3 weeks, the cultivation environment temperature is 20-25 ℃, the cultivation environment relative humidity is 65% -85%, the rooting agent for flower and seedling cuttage with the concentration of 30% -40% is used for one time every week in the cultivation period, and the spraying time is morning or evening.
6. The rooting agent for flower and seedling cuttage according to claim 3, wherein the cuttage matrix is turfy soil, and the transplanting matrix is one or a combination of more of loess, turfy soil and diatomite.
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