CN106800490A - A kind of red kiwi fruit biological compound fertilizer for increasing fruit sweetness - Google Patents
A kind of red kiwi fruit biological compound fertilizer for increasing fruit sweetness Download PDFInfo
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- CN106800490A CN106800490A CN201710117956.3A CN201710117956A CN106800490A CN 106800490 A CN106800490 A CN 106800490A CN 201710117956 A CN201710117956 A CN 201710117956A CN 106800490 A CN106800490 A CN 106800490A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention belongs to plant composite fertilizer's applied technical field, disclosing a kind of red kiwi fruit increases the biological compound fertilizer of fruit sweetness, including following parts by weight component：Escherichia coli, bacillus, sulfur oxidizing bacterium, nitrobacteria, Dry chick faeces just, dry cow dung just, moisture；Also disclose it and specifically include following preparation process：The first step：Escherichia coli and the culture of sulfur oxidizing bacterium；Second step：Bacillus and the culture of nitrobacteria；3rd step：Mixed culture.The present invention passes through fermentation of the biological flora organic complex to organic fertilizer, obtain composite microbiological fertilizer, both the bioavailability of composite microbic bacterial fertilizer can have been improved, it is capable of the resistance of plant again, reduce the P of plant, the sugariness and yield of fruit are improved, the economic benefit of red kiwi fruit plantation is improved.
Description
Technical field
It is combined with the biology for increasing fruit sweetness the present invention relates to agricultural fertilizer field, more particularly to a kind of red kiwi fruit
Fertilizer, also discloses its preparation method.
Background technology
Red kiwi fruit also known as red heart Chinese grooseberry/red kiwifruit, characteristic are big in fruit, neat；Fruit is short cylindrical
Shape, pericarp is in green and brown color, without hair.Containing rich in sugar, rich in the several mineral materials such as calcium, iron, potassium and 17 kinds of amino acid, Vc is up to
135.Pulp is emerald green, and the sweet acid of fruit juice is moderate, and pleasant to the palate, quality is extremely excellent.It is direct-edible, can also be adapted to manufacture craft dish
Meat and fish dishes.
The growing environment of Kiwi berry, distribution of the root system in hard soil layer is shallower, can be distributed in loose soil compared with
Deep characteristic, can well-grown in acid, subacidity (soil pH 5.5-6.5) or neutral soil.Existing plurality area
The Kiwi berry of plantation, because of improper administration chemical fertilizer, agricultural chemicals and swelling agent year after year, often leads to that fruit quality is poor, and yielding ability is not strong, produces
Amount is relatively low, and disease resistance poor performance, and such as bacterial canker and fungal leaf spot have a strong impact on the yield and product of Kiwi berry
Matter.
Chinese invention patent CN102060629A is related to a kind of special medicated fertilizer for potatoes and preparation method thereof, the medicine fertilizer to be
It is mixed into agricultural chemicals in organic fertilizer to be made, the formula composition of the agricultural chemicals is：2% kasugarnycin wettable powder, DT wettable powders
Agent, 50% Phoxim emulsion and chlopyrifos emulsion, the mass fraction of each composition is 25-35 parts of 2% kasugarnycin wettable powder,
25-35 parts of DT wettable powders, 50% Phoxim emulsion 15-25 parts and 15-25 parts of chlopyrifos emulsion.By by fertilizer and agricultural chemicals
Organically it is combined together, using more convenient.The underground such as potato black rot and cutworm pest and disease damage can be significantly prevented and treated, is had
Effect ground improves the commodity of potato emergence rate and potato, but the present invention does not have particularity for cauliflower growth, no
Beneficial to the growth demand of cauliflower, while inorganic and organic agricultural chemicals composition is added in the invention, it is unfavorable for the improvement of soil quality, it is right
Ecological environment causes certain adverse effect.
Chinese invention patent CN101817707A discloses a kind of bioorganic pesticide fertilizer and its production method, the biological organic
Medicine fertilizer is the agricultural crop straw or fermentation bed for pig raising bedding and padding become thoroughly decomposed by 70-80%, 5-10% medical stones, the inorganic compounds of 15-25%
Fertilizer, the mix products of gained after being fermented through effective microbe, effective microbe quantity is 2-3 × 108CFU/g after fermentation.By corruption
After ripe agricultural crop straw or fermentation bed for pig raising bedding and padding mixes with medical stone, the inorganic composite mixed fertilizer of 5-10%, Ran Houtian are first added
Fermented at normal temperatures added with effect microorganism fungus kind, the inorganic composite mixed fertilizer of remaining 10-15% is finally added to above-mentioned hair
Obtained final product in ferment thing.The bioorganic pesticide fertilizer of inventive method production can improve the yield of crop, can be to crucifer disease
Evil plays preferable prevention effect, additionally it is possible to reduce the nitrate content in brassicaceous vegetable, improving quality, production mistake
Agricultural wastes are further converted in journey, the invention biological organic fertilizer is mainly by effective flora to Cruciferae fabric
Good antibacterial resistant effect is produced, does not have good preventive and therapeutic effect for the disease that the insect pests such as cabbageworm, aphid are produced.
The content of the invention
The problem that the present invention exists for prior art, there is provided a kind of red kiwi fruit is answered with the biology for increasing fruit sweetness
Hefei, by the fermentation after the organic complex of biological flora to organic fertilizer, obtains composite microbiological fertilizer, can both improve multiple
The bioavailability of microbial-bacterial fertilizer is closed, the resistance of plant is capable of again, reduce the P of plant, improve the sweet of fruit
Degree, while a plant yield can also be improved, improves the economic benefit of red kiwi fruit plantation.
The present invention provides following technical scheme：
A kind of red kiwi fruit biological compound fertilizer for increasing fruit sweetness, including following components：
Escherichia coli, bacillus, sulfur oxidizing bacterium, nitrobacteria, Dry chick faeces just, dry cow dung just, water；
Specifically include following preparation process：
The first step：Escherichia coli and the culture of sulfur oxidizing bacterium：
15g nutrient broth mediums are weighed in 500ml distilled water, 0.1-1g metronidazole solids are added, stirred through vibration
After be put into sterilizing in vertical pressure steam sterilizer, to open and open ultraviolet after superclean bench and carry out sterilized 10-
20min, is placed into superclean bench after culture medium after high pressure sterilization, is taken out, and waits the temperature of culture medium to drop to a 60-70 DEG C of left side
The right side, under sterile conditions, Escherichia coli is inoculated into culture medium, has been inoculated with Escherichia coli and has been continued to be inoculated with sulfur oxidizing bacterium, so
After be put into biochemical cultivation case and incubator temperature be adjusted to 37-38 DEG C, cultivate 8-10h；
Second step：Bacillus and the culture of nitrobacteria：
15g nutrient broth mediums are weighed in 500ml distilled water, vertical pressure steam sterilizing is put into after vibration stirring
Sterilizing in device, opening ultraviolet carries out sterilized 20-30min after opening superclean bench, after after high pressure sterilization, takes out culture
Base is placed into superclean bench, waits the temperature of culture medium to drop to 50-60 DEG C or so, under sterile conditions, by bacillus
It is inoculated into culture medium, the mixing of 0.1-1g nikethamidums solution is added after being inoculated with bacillus, after continuing to be inoculated with nitrobacteria
It is put into biochemical cultivation case and incubator temperature is adjusted to 35-37 DEG C, cultivates 8-10h；
3rd step：Mixed culture：
Viable count is more than or equal to 1 × 10 in nutrient solution to be mixed8After individual/ml, taken out with pipettor be inoculated into drying respectively
Chicken manure with dry cow dung just and mixed fertilizer that water is mixed in, be well mixed, be collectively disposed in 37 DEG C of incubator and train
Sealed after supporting 3-5 days and be made.
Preferably, in the incubation of the Escherichia coli and sulfur oxidizing bacterium, the Escherichia coli connect with sulfur oxidizing bacterium
The amount of kind is 10：1.
Preferably, in the incubation of the bacillus and nitrobacteria, the bacillus and nitrobacteria
Inoculum concentration is 1:10.
Preferably, 15g nutrient broth mediums are weighed in the first step preparation process in 500ml distilled water, is added
0.1-0.5g metronidazole solids.
Preferably, addition 0.5-1g nikethamidums solution is mixed after bacillus has been inoculated with the second step preparation process
It is even, continue to be inoculated with nitrobacteria.
Preferably, Escherichia coli and the inoculum concentration of sulfur oxidizing bacterium are 1g/Kg, bacillus in the 3rd step preparation process
And the inoculum concentration of nitrobacteria is 1.5g/Kg..
The characteristics of biological organic fertilizer：1 improves chemical fertilizer utilization ratio, and biological organic fertilizer has fixed nitrogen, phosphorus decomposing, potassium decomposing etc. to act on, can
To greatly improve the utilization rate of chemical fertilizer.2 improved soils, increase soil fertility, not only organic component content is rich in biological organic fertilizer
Richness, the beneficial bacterium for being also conducive to soil environment to improve in addition.After soil application biological organic fertilizer, largely increased
The content of the nutriments such as organic matter, nitrogen, phosphorus, the potassium of soil, phosphorus decomposing, potassium solubilizing bacteria in fertilizer played in soil its phosphorus decomposing,
Potassium decomposing is acted on, and the P elements and potassium element in soil are discharged, and increased the available content of mineral substances of plant in soil.
On the other hand, because biological organic fertilizer is applied in soil, the organic matter content in soil is increased, soil is become thin
Pine, nutrient laden, the original flora lived in soil obtains amount reproduction, so as to ensure that the micro-ecological environment of soil.3 mitigate
Environmental pollution.4 improve crop quality, and biological organic fertilizer is rich in the multiple nutritional components having and imparts the special fertilizer efficiency of fertilizer, passes through
Research has shown that biological organic fertilizer can improve the qualities such as color and luster, mouthfeel and the growth vigor of crop.5 mitigate crop pests, one
It is the bacteriostasis of biological organic fertilizer itself, two is effect of biological organic fertilizer on crops growth, antibacterial, disease-resistant facilitation.
Metronidazole is nitro imidazole derivatives, can suppress Amoeba redox reaction, protozoon nitrogen chain occurs disconnected
Split.In vitro test is proved, when drug concentration is l-2mg/L, amoeba histolytica can occur morphologic change, 24 in 6-20 hours
All it is killed in hour, when concentration is 0.2g/ml, amoeba histolytica can be killed in 72 hours.
Metronidazole plays the role of powerful killing trichomonad, and its mechanism is unknown.Antimicrobial spectrum includes bacteroides fragilis and other classes
Bacillus, fusiform bacilarmature, aerogenesis bacillus, Eubacterium, Peptococcus and Peptostreptococcus etc..Its bacteriocidal concentration is slightly higher
In Mlc.Metronidazole plays killing action to the cell grown under anaerobic condition and anaerobe, and it is reduced in human body
The metabolin of Shi Shengcheng, it may have anaerobe resistant is acted on, but to aerobic bacteria and facultative anaerobic bacteria without effect.The nitro of anaerobic bacteria is also
Protoenzyme is played an important role in the energetic supersession of sensitive strain, and metronidazole is applied to the present invention incubation of microorganism, warp
The metronidazole crossed after high-temperature process does not only play a part of Developing restraint to Escherichia coli and sulfur oxidizing bacterium, and serves
Promote it to grow, and improve Escherichia coli and decomposition of the sulfur oxidizing bacterium to animal wastes organic fertilizer, improve most
There is fabulous facilitation in whole composite fertilizer to the growth-promoting effect of kiwi fruit plant for the sugariness of raising kiwifruit fruit.
Nikethamidum is bulbar excitant.There is direct excitation to respiratory center, deepen accelerated breathing, its effect is fast
Fast, gentle, safe range is larger, also has weaker excitation to cerebral cortex, vasomotor center etc..For a variety of causes
The respiration inhibition for causing, confrontation morphinism effect is preferable；Stopping caused by Central nervous dyshaemia such as wound or operation
Gram, the heart failure of acute infectious disease and collapse etc.；Can also be used for asphyxia neonatorum, premature's expiratory dyspnea and various chronic cardiacs
Illness, expiratory dyspnea etc..Nikethamidum is applied to agricultural planting fertilizer utilization field by the present invention, by inoculation of medium gemma
0.1-1g nikethamidums solution is added after bacillus to mix, continues to be inoculated with nitrobacteria, can improve bacillus and nitrobacteria
The activity for coexisting culture efficiency, improving thalline, improve efficiency of the mixed bacterial for animal wastes compost fermentation, improve high temperature
The temperature and time length of phase, increases fermentation process of the flora for organic substance, improves final products for kiwi fruit plant
Growth-promoting effect, reduce plant the incidence of disease, improve Kiwi berry plantation economic benefit and ecological benefits.
Beneficial effects of the present invention are：
1. the present invention provides a kind of biological compound fertilizer of red kiwi fruit with fruit sweetness is increased, by having for biological flora
Fermentation after machine compounding to organic fertilizer, obtains composite microbiological fertilizer, can both improve the biology profit of composite microbic bacterial fertilizer
With rate, resistance of the microbial-bacterial fertilizer for plant can be improved again, reduce the P of plant, improve red kiwi fruit kind
The economic benefit of plant.
2. metronidazole is applied to the present invention incubation of microorganism, is not had not only by the metronidazole after high-temperature process
Escherichia coli and sulfur oxidizing bacterium are played a part of with Developing restraint, and it grows to serve promotion, and improve large intestine
Bacillus and decomposition of the sulfur oxidizing bacterium to animal wastes organic fertilizer, improve growth of the final composite fertilizer to kiwi fruit plant and promote
Enter effect, the sugariness for improving kiwifruit fruit has fabulous facilitation.
3. nikethamidum is applied to agricultural planting fertilizer utilization field by the present invention, after inoculation of medium bacillus
Addition 0.1-1g nikethamidums solution is mixed, and continues to be inoculated with nitrobacteria, can improve coexisting for bacillus and nitrobacteria
Culture efficiency, improves the activity of thalline, improves efficiency of the mixed bacterial for animal wastes compost fermentation, improves the temperature of megathermal period
Degree and time span, increase fermentation process of the flora for organic substance, improve growth of the final products for kiwi fruit plant
Facilitation, improves fruit sweetness, reduces the incidence of disease of plant, improves the economic benefit and ecological benefits of Kiwi berry plantation.
4. composite microbiological fertilizer of the present invention is fermented by During High-Temperature Composting and is made, and can promote kiwi fruit plant to nutrition unit
Element absorb and tangerine resists the pathogenic effects of some pathogenic microorganisms, mitigate the pest and disease damage in Kiwi berry planting process, carry
Height plantation quality and yield, improve economy and zoology benifit.
Specific embodiment
Embodiments of the invention are described in detail below, the present embodiment is carried out under premised on inventive technique scheme
Implement, give detailed implementation method and specific operating process, but protection scope of the present invention is not limited to following realities
Apply example.The experimental program of unreceipted actual conditions in embodiment, generally according to the condition proposed by normal condition or manufacturer
Implement.
A kind of red kiwi fruit biological compound fertilizer for increasing fruit sweetness, including following components：
Escherichia coli, bacillus, sulfur oxidizing bacterium, nitrobacteria, Dry chick faeces just, dry cow dung just, water.
Embodiment one
Specifically include following preparation process：
The first step：Escherichia coli and the culture of sulfur oxidizing bacterium：
15g nutrient broth mediums are weighed in 500ml distilled water, 0.1g metronidazole solids are added, after vibration stirring
Sterilizing in vertical pressure steam sterilizer is put into, opening ultraviolet carries out sterilized 10min after opening superclean bench, treats height
After pressure sterilization, take out culture medium and be placed into superclean bench, wait the temperature of culture medium to drop to 60 DEG C or so, in aseptic condition
Under, Escherichia coli are inoculated into culture medium, it has been inoculated with Escherichia coli and has continued to be inoculated with sulfur oxidizing bacterium, it is then placed in biochemical cultivation case
In and incubator temperature is adjusted to 37 DEG C, cultivate 8h；
Second step：Bacillus and the culture of nitrobacteria：
15g nutrient broth mediums are weighed in 500ml distilled water, vertical pressure steam sterilizing is put into after vibration stirring
Sterilizing in device, opening ultraviolet carries out sterilized 20min after opening superclean bench, after after high pressure sterilization, takes out culture medium
It is placed into superclean bench, waits the temperature of culture medium to drop to 50 DEG C or so, under sterile conditions, bacillus is inoculated into
In culture medium, the mixing of 0.1g nikethamidums solution is added after being inoculated with bacillus, continue to be put into biochemistry after being inoculated with nitrobacteria
35 DEG C are adjusted in incubator and by incubator temperature, 8h is cultivated；
3rd step：Mixed culture：
Viable count is more than or equal to 1 × 10 in nutrient solution to be mixed8After individual/ml, taken out with pipettor be inoculated into drying respectively
Chicken manure with dry cow dung just and mixed fertilizer that water is mixed in, be well mixed, be collectively disposed in 37 DEG C of incubator and train
Sealed after supporting 3 days and be made.
Embodiment two
Specifically include following preparation process：
The first step：Escherichia coli and the culture of sulfur oxidizing bacterium：
15g nutrient broth mediums are weighed in 500ml distilled water, 1g metronidazole solids are added, put after vibration stirring
Enter sterilizing in vertical pressure steam sterilizer, opening ultraviolet carries out sterilized 20min after opening superclean bench, treats high pressure
After sterilization, take out culture medium and be placed into superclean bench, wait the temperature of culture medium to drop to 70 DEG C or so, in aseptic condition
Under, Escherichia coli are inoculated into culture medium, it has been inoculated with Escherichia coli and has continued to be inoculated with sulfur oxidizing bacterium, it is then placed in biochemical cultivation case
In and incubator temperature is adjusted to 38 DEG C, cultivate 10h；
Second step：Bacillus and the culture of nitrobacteria：
15g nutrient broth mediums are weighed in 500ml distilled water, vertical pressure steam sterilizing is put into after vibration stirring
Sterilizing in device, opening ultraviolet carries out sterilized 30min after opening superclean bench, after after high pressure sterilization, takes out culture medium
It is placed into superclean bench, waits the temperature of culture medium to drop to 60 DEG C or so, under sterile conditions, bacillus is inoculated into
In culture medium, the mixing of 1g nikethamidums solution is added after being inoculated with bacillus, continue to be put into biochemical training after being inoculated with nitrobacteria
37 DEG C are adjusted in foster case and by incubator temperature, 10h is cultivated；
3rd step：Mixed culture：
Viable count is more than or equal to 1 × 10 in nutrient solution to be mixed8After individual/ml, taken out with pipettor be inoculated into drying respectively
Chicken manure with dry cow dung just and mixed fertilizer that water is mixed in, be well mixed, be collectively disposed in 37 DEG C of incubator and train
Sealed after supporting 5 days and be made.
Embodiment three
Specifically include following preparation process：
The first step：Escherichia coli and the culture of sulfur oxidizing bacterium：
15g nutrient broth mediums are weighed in 500ml distilled water, 0.5g metronidazole solids are added, after vibration stirring
Sterilizing in vertical pressure steam sterilizer is put into, opening ultraviolet carries out sterilized 10min after opening superclean bench, treats height
After pressure sterilization, take out culture medium and be placed into superclean bench, wait the temperature of culture medium to drop to 70 DEG C or so, in aseptic condition
Under, Escherichia coli are inoculated into culture medium, it has been inoculated with Escherichia coli and has continued to be inoculated with sulfur oxidizing bacterium, it is then placed in biochemical cultivation case
In and incubator temperature is adjusted to 37 DEG C, cultivate 10h；
Second step：Bacillus and the culture of nitrobacteria：
15g nutrient broth mediums are weighed in 500ml distilled water, vertical pressure steam sterilizing is put into after vibration stirring
Sterilizing in device, opening ultraviolet carries out sterilized 20min after opening superclean bench, after after high pressure sterilization, takes out culture medium
It is placed into superclean bench, waits the temperature of culture medium to drop to 60 DEG C or so, under sterile conditions, bacillus is inoculated into
In culture medium, the mixing of 0.1g nikethamidums solution is added after being inoculated with bacillus, continue to be put into biochemistry after being inoculated with nitrobacteria
37 DEG C are adjusted in incubator and by incubator temperature, 8h is cultivated；
3rd step：Mixed culture：
Viable count is more than or equal to 1 × 10 in nutrient solution to be mixed8After individual/ml, taken out with pipettor be inoculated into drying respectively
Chicken manure with dry cow dung just and mixed fertilizer that water is mixed in, be well mixed, be collectively disposed in 37 DEG C of incubator and train
Sealed after supporting 5 days and be made.
Example IV
Specifically include following preparation process：
The first step：Escherichia coli and the culture of sulfur oxidizing bacterium：
15g nutrient broth mediums are weighed in 500ml distilled water, 0.5g metronidazole solids are added, after vibration stirring
Sterilizing in vertical pressure steam sterilizer is put into, opening ultraviolet carries out sterilized 15min after opening superclean bench, treats height
After pressure sterilization, take out culture medium and be placed into superclean bench, wait the temperature of culture medium to drop to 70 DEG C or so, in aseptic condition
Under, Escherichia coli are inoculated into culture medium, it has been inoculated with Escherichia coli and has continued to be inoculated with sulfur oxidizing bacterium, it is then placed in biochemical cultivation case
In and incubator temperature is adjusted to 37 DEG C, cultivate 10h；
Second step：Bacillus and the culture of nitrobacteria：
15g nutrient broth mediums are weighed in 500ml distilled water, vertical pressure steam sterilizing is put into after vibration stirring
Sterilizing in device, opening ultraviolet carries out sterilized 20min after opening superclean bench, after after high pressure sterilization, takes out culture medium
It is placed into superclean bench, waits the temperature of culture medium to drop to 60 DEG C or so, under sterile conditions, bacillus is inoculated into
In culture medium, the mixing of 0.1g nikethamidums solution is added after being inoculated with bacillus, continue to be put into biochemistry after being inoculated with nitrobacteria
37 DEG C are adjusted in incubator and by incubator temperature, 8h is cultivated；
3rd step：Mixed culture：
Viable count is more than or equal to 1 × 10 in nutrient solution to be mixed8After individual/ml, taken out with pipettor be inoculated into drying respectively
Chicken manure with dry cow dung just and mixed fertilizer that water is mixed in, be well mixed, be collectively disposed in 37 DEG C of incubator and train
Sealed after supporting 5 days and be made.
Embodiment five
Specifically include following preparation process：
The first step：Escherichia coli and the culture of sulfur oxidizing bacterium：
15g nutrient broth mediums are weighed in 500ml distilled water, 0.5g metronidazole solids are added, after vibration stirring
Sterilizing in vertical pressure steam sterilizer is put into, opening ultraviolet carries out sterilized 15min after opening superclean bench, treats height
After pressure sterilization, take out culture medium and be placed into superclean bench, wait the temperature of culture medium to drop to 65 DEG C or so, in aseptic condition
Under, Escherichia coli are inoculated into culture medium, it has been inoculated with Escherichia coli and has continued to be inoculated with sulfur oxidizing bacterium, it is then placed in biochemical cultivation case
In and incubator temperature is adjusted to 38 DEG C, cultivate 9h；
Second step：Bacillus and the culture of nitrobacteria：
15g nutrient broth mediums are weighed in 500ml distilled water, vertical pressure steam sterilizing is put into after vibration stirring
Sterilizing in device, opening ultraviolet carries out sterilized 25min after opening superclean bench, after after high pressure sterilization, takes out culture medium
It is placed into superclean bench, waits the temperature of culture medium to drop to 55 DEG C or so, under sterile conditions, bacillus is inoculated into
In culture medium, the mixing of 0.5g nikethamidums solution is added after being inoculated with bacillus, continue to be put into biochemistry after being inoculated with nitrobacteria
37 DEG C are adjusted in incubator and by incubator temperature, 9h is cultivated；
3rd step：Mixed culture：
Viable count is more than or equal to 1 × 10 in nutrient solution to be mixed8After individual/ml, taken out with pipettor be inoculated into drying respectively
Chicken manure with dry cow dung just and mixed fertilizer that water is mixed in, be well mixed, be collectively disposed in 37 DEG C of incubator and train
Sealed after supporting 5 days and be made.
Embodiment six
Specifically include following preparation process：
The first step：Escherichia coli and the culture of sulfur oxidizing bacterium：
15g nutrient broth mediums are weighed in 500ml distilled water, 0.6g metronidazole solids are added, after vibration stirring
Sterilizing in vertical pressure steam sterilizer is put into, opening ultraviolet carries out sterilized 15min after opening superclean bench, treats height
After pressure sterilization, take out culture medium and be placed into superclean bench, wait the temperature of culture medium to drop to 70 DEG C or so, in aseptic condition
Under, Escherichia coli are inoculated into culture medium, it has been inoculated with Escherichia coli and has continued to be inoculated with sulfur oxidizing bacterium, it is then placed in biochemical cultivation case
In and incubator temperature is adjusted to 37 DEG C, cultivate 9h；
Second step：Bacillus and the culture of nitrobacteria：
15g nutrient broth mediums are weighed in 500ml distilled water, vertical pressure steam sterilizing is put into after vibration stirring
Sterilizing in device, opening ultraviolet carries out sterilized 25min after opening superclean bench, after after high pressure sterilization, takes out culture medium
It is placed into superclean bench, waits the temperature of culture medium to drop to 55 DEG C or so, under sterile conditions, bacillus is inoculated into
In culture medium, the mixing of 0.6g nikethamidums solution is added after being inoculated with bacillus, continue to be put into biochemistry after being inoculated with nitrobacteria
36 DEG C are adjusted in incubator and by incubator temperature, 10h is cultivated；
3rd step：Mixed culture：
Viable count is more than or equal to 1 × 10 in nutrient solution to be mixed8After individual/ml, taken out with pipettor be inoculated into drying respectively
Chicken manure with dry cow dung just and mixed fertilizer that water is mixed in, be well mixed, be collectively disposed in 37 DEG C of incubator and train
Sealed after supporting 3 days and be made.
Comparative example one
Specifically include following preparation process：
The first step：Escherichia coli and the culture of sulfur oxidizing bacterium：
15g nutrient broth mediums are weighed in 500ml distilled water, vertical pressure steam sterilizing is put into after vibration stirring
Sterilizing in device, opening ultraviolet carries out sterilized 10min after opening superclean bench, after after high pressure sterilization, takes out culture medium
It is placed into superclean bench, waits the temperature of culture medium to drop to 70 DEG C or so, under sterile conditions, Escherichia coli is inoculated into
In culture medium, Escherichia coli have been inoculated with it and have continued to be inoculated with sulfur oxidizing bacterium, has been then placed in biochemical cultivation case and adjusts incubator temperature
To 37 DEG C, 10h is cultivated；
Second step：Bacillus and the culture of nitrobacteria：
15g nutrient broth mediums are weighed in 500ml distilled water, vertical pressure steam sterilizing is put into after vibration stirring
Sterilizing in device, opening ultraviolet carries out sterilized 20min after opening superclean bench, after after high pressure sterilization, takes out culture medium
It is placed into superclean bench, waits the temperature of culture medium to drop to 50 DEG C or so, under sterile conditions, bacillus is inoculated into
In culture medium, it has been inoculated with after the follow-up continued access kind nitrobacteria of bacillus and has been put into biochemical cultivation case and is adjusted to incubator temperature
37 DEG C, cultivate 9h；
3rd step：Mixed culture：
Viable count is more than or equal to 1 × 10 in nutrient solution to be mixed8After individual/ml, taken out with pipettor be inoculated into drying respectively
Chicken manure with dry cow dung just and mixed fertilizer that water is mixed in, be well mixed, be collectively disposed in 37 DEG C of incubator and train
Support 4 days.
Comparative example two
Specifically include following preparation process：
The first step：Escherichia coli and the culture of sulfur oxidizing bacterium：
15g nutrient broth mediums are weighed in 500ml distilled water, vertical pressure steam sterilizing is put into after vibration stirring
Sterilizing in device, opening ultraviolet carries out sterilized 10min after opening superclean bench, after after high pressure sterilization, takes out culture medium
It is placed into superclean bench, waits the temperature of culture medium to drop to 70 DEG C or so, under sterile conditions, Escherichia coli is inoculated into
In culture medium, Escherichia coli have been inoculated with it and have continued to be inoculated with sulfur oxidizing bacterium, has been then placed in biochemical cultivation case and adjusts incubator temperature
To 37 DEG C, 10h is cultivated；
Second step：Bacillus and the culture of nitrobacteria：
15g nutrient broth mediums are weighed in 500ml distilled water, vertical pressure steam sterilizing is put into after vibration stirring
Sterilizing in device, opening ultraviolet carries out sterilized 20min after opening superclean bench, after after high pressure sterilization, takes out culture medium
It is placed into superclean bench, waits the temperature of culture medium to drop to 50 DEG C or so, under sterile conditions, bacillus is inoculated into
In culture medium, the mixing of 0.5g nikethamidums solution is added after being inoculated with bacillus, continue to be put into biochemistry after being inoculated with nitrobacteria
37 DEG C are adjusted in incubator and by incubator temperature, 9h is cultivated；
3rd step：Mixed culture：
Viable count is more than or equal to 1 × 10 in nutrient solution to be mixed8After individual/ml, taken out with pipettor be inoculated into drying respectively
Chicken manure with dry cow dung just and mixed fertilizer that water is mixed in, be well mixed, be collectively disposed in 37 DEG C of incubator and train
Support 4 days.
Comparative example three
Specifically include following preparation process：
The first step：Escherichia coli and the culture of sulfur oxidizing bacterium：
15g nutrient broth mediums are weighed in 500ml distilled water, 0.5g metronidazole solids are added, after vibration stirring
Sterilizing in vertical pressure steam sterilizer is put into, opening ultraviolet carries out sterilized 10min after opening superclean bench, treats height
After pressure sterilization, take out culture medium and be placed into superclean bench, wait the temperature of culture medium to drop to 70 DEG C or so, in aseptic condition
Under, Escherichia coli are inoculated into culture medium, it has been inoculated with Escherichia coli and has continued to be inoculated with sulfur oxidizing bacterium, it is then placed in biochemical cultivation case
In and incubator temperature is adjusted to 37 DEG C, cultivate 10h；
Second step：Bacillus and the culture of nitrobacteria：
15g nutrient broth mediums are weighed in 500ml distilled water, vertical pressure steam sterilizing is put into after vibration stirring
Sterilizing in device, opening ultraviolet carries out sterilized 20min after opening superclean bench, after after high pressure sterilization, takes out culture medium
It is placed into superclean bench, waits the temperature of culture medium to drop to 50 DEG C or so, under sterile conditions, bacillus is inoculated into
In culture medium, it has been inoculated with after the follow-up continued access kind nitrobacteria of bacillus and has been put into biochemical cultivation case and is adjusted to incubator temperature
37 DEG C, cultivate 9h；
3rd step：Mixed culture：
Viable count is more than or equal to 1 × 10 in nutrient solution to be mixed8After individual/ml, taken out with pipettor be inoculated into drying respectively
Chicken manure with dry cow dung just and mixed fertilizer that water is mixed in, be well mixed, be collectively disposed in 37 DEG C of incubator and train
Support 4 days.
In the incubation of the Escherichia coli and sulfur oxidizing bacterium, the Escherichia coli are with the inoculum concentration of sulfur oxidizing bacterium
10：1.
In the incubation of the bacillus and nitrobacteria, the bacillus is with the inoculum concentration of nitrobacteria
1:10。
Escherichia coli and the inoculum concentration of sulfur oxidizing bacterium are 1g/Kg, bacillus and nitrification in the 3rd step preparation process
The inoculum concentration of bacterium is 1.5g/Kg.
Planting experiment one
Ningbo of Zhejiang north logical sequence planting base carries out 5 years seedling planting experiments of red kiwi fruit, and amount of application is 1kg/ plants.Will experiment
Field is randomly divided into 90 parts, then it is one group to extract 10 parts in 90 parts at random, and experimental plot is divided into 9 groups altogether, and embodiment is denoted as respectively
One to six group and comparative example one, comparative example two, comparative example three, the average strain yield of kiwi fruit plant of statistics use different fertilizer,
The incidence of disease and pol content.
Table one：Ningbo red kiwi fruit yield, the disease incidence of disease and pol
| Planting experiment one | Yield (kilogram/plant) | The incidence of disease (%) | Pol (%) |
| Embodiment one | 46.52 | 0.53 | 14.79 |
| Embodiment two | 47.67 | 0.87 | 15.25 |
| Embodiment three | 50.00 | 0.98 | 16.20 |
| Example IV | 48.65 | 0.69 | 16.61 |
| Embodiment five | 49.97 | 0.90 | 16.53 |
| Embodiment six | 50.07 | 0.57 | 15.71 |
| Comparative example one | 30.53 | 1.92 | 12.04 |
| Comparative example two | 31.37 | 2.51 | 11.65 |
| Comparative example three | 40.36 | 1.02 | 11.36 |
Planting experiment two
Carried out in Suzhou Dongshan, 4 years seedling planting experiments of red kiwi fruit, amount of application is 1kg/ plants.Experimental plot is divided at random
90 parts, then at random in 90 parts extract 10 parts be one group, experimental plot is divided into 9 groups altogether, be denoted as respectively the group of embodiment one to six and
Comparative example one, comparative example two, comparative example three, statistics use the average strain yield of kiwi fruit plant, the incidence of disease and the sugar of different fertilizer
Degree content.
Table two：Suzhou red kiwi fruit yield, the disease incidence of disease and pol
Above content is only better embodiment of the invention, for one of ordinary skill in the art, according to the present invention
Thought, will change in specific embodiments and applications, this specification content should not be construed as to this hair
Bright limitation.
Claims (6)
1. a kind of red kiwi fruit use increases the biological compound fertilizer of fruit sweetness, it is characterised in that：
Including following components：
Escherichia coli, bacillus, sulfur oxidizing bacterium, nitrobacteria, Dry chick faeces just, dry cow dung just, water；
Specifically include following preparation process：
The first step：Escherichia coli and the culture of sulfur oxidizing bacterium：
15g nutrient broth mediums are weighed in 500ml distilled water, 0.1-1g metronidazole solids are added, put after vibration stirring
Enter sterilizing in vertical pressure steam sterilizer, opening ultraviolet carries out sterilized 10-20min after opening superclean bench, treats
After high pressure sterilization, take out culture medium and be placed into superclean bench, wait the temperature of culture medium to drop to 60-70 DEG C or so, aseptic
Under conditions of, Escherichia coli are inoculated into culture medium, it has been inoculated with Escherichia coli and has continued to be inoculated with sulfur oxidizing bacterium, it is then placed in biochemistry
37-38 DEG C is adjusted in incubator and by incubator temperature, 8-10h is cultivated；
Second step：Bacillus and the culture of nitrobacteria：
15g nutrient broth mediums are weighed in 500ml distilled water, is put into vertical pressure steam sterilizer after vibration stirring
Sterilizing, opening ultraviolet carries out sterilized 20-30min after opening superclean bench, is put after culture medium after high pressure sterilization, is taken out
Put in superclean bench, wait the temperature of culture medium to drop to 50-60 DEG C or so, under sterile conditions, bacillus is inoculated with
To in culture medium, the mixing of 0.1-1g nikethamidums solution is added after being inoculated with bacillus, continue to be put into after being inoculated with nitrobacteria
35-37 DEG C is adjusted in biochemical cultivation case and by incubator temperature, 8-10h is cultivated；
3rd step：Mixed culture：
Viable count is more than or equal to 1 × 10 in nutrient solution to be mixed8After individual/ml, taken out with pipettor be inoculated into Dry chick faeces just respectively
With dry cow dung just and mixed fertilizer that water is mixed in, be well mixed, be collectively disposed in 37 DEG C of incubator and cultivate 3-5 days
Sealing afterwards is made.
2. a kind of red kiwi fruit use according to claim 1 increases the biological compound fertilizer of fruit sweetness, it is characterised in that：
In the incubation of the Escherichia coli and sulfur oxidizing bacterium, the Escherichia coli are 10 with the inoculum concentration of sulfur oxidizing bacterium：1.
3. a kind of red kiwi fruit use according to claim 1 increases the biological compound fertilizer of fruit sweetness, it is characterised in that：
In the incubation of the bacillus and nitrobacteria, the bacillus is 1 with the inoculum concentration of nitrobacteria:
10。
4. a kind of red kiwi fruit use according to claim 1 increases the biological compound fertilizer of fruit sweetness, it is characterised in that：
15g nutrient broth mediums are weighed in the first step preparation process in 500ml distilled water, 0.1-0.5g first nitre is added
Azoles solid.
5. a kind of red kiwi fruit use according to claim 1 increases the biological compound fertilizer of fruit sweetness, it is characterised in that：
The mixing of 0.5-1g nikethamidums solution is added after bacillus has been inoculated with the second step preparation process, continues to be inoculated with nitre
Change bacterium.
6. a kind of red kiwi fruit use according to claim 1 increases the biological compound fertilizer of fruit sweetness, it is characterised in that：
Escherichia coli and the inoculum concentration of sulfur oxidizing bacterium are 1g/Kg, bacillus and nitrobacteria in the 3rd step preparation process
Inoculum concentration be 1.5g/Kg.
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Cited By (1)
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| CN105481580A (en) * | 2015-12-29 | 2016-04-13 | 广元市牛牛生物科技有限公司 | Novel organic fertilizer for kiwifruits and preparation method thereof |
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| CN101348388A (en) * | 2008-07-25 | 2009-01-21 | 李海泉 | Preparation of flavoured bacterial manure containing fermented chicken manure |
| CN103725634A (en) * | 2013-12-31 | 2014-04-16 | 陕西万源生物农业科技有限公司 | Compound probiotics for planting kiwi fruits |
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