Special complete first biological organic fertilizer of a kind of leaf vegetables and preparation method thereof
Technical field
The present invention relates to agricultural biological technical fields, and in particular to a kind of special complete first biological organic fertilizer of leaf vegetables and its preparation
Method.
Background technology
Leaf vegetables is using the tender blade of vegetable fertilizer and petiole as the vegetables of Leaf-feeding insects, and leaf vegetables type is more, plantation
Period is short, and planting patterns is flexible, planting season wide adaptability, and planting benefit is high, therefore leaf vegetables crop production is China's plantation
The important component of industry is to improve agricultural production efficiency, one of important channel increased farmers' income.As China's agricultural is fast
Speed development, leaf vegetables large-scale planting quickly grows, at present leaf vegetables apply fertilizer mainly and include inorganic fertilizer, organic fertilizer and
Microorganism fertilizer.Wherein, inorganic fertilizer is rich in quick-acting nitrogen, phosphorus, potassium, and fertilizer efficiency is fast, effect of increasing production is apparent, is used in leaf vegetables is planted
It is larger, but long-term single use chemical fertilizer, it be easy to cause soil hardening, soil physico-chemical property change, quality of agricultural product decline etc. no
Good consequence.Application of organic fertilizers can soil fertility raising, improvement physicochemical character, prevention plant soil-borne diseases, raising agricultural output
With quality, but available nutrient content is relatively low in organic fertilizer, and fertilizer efficiency is slower, it is difficult to meet the growth demand of crop.Microorganism fertilizer
The conversion of soil nutrient can be accelerated by including functional microbial bacterial agent, enhance soil fertility, but growth, the breeding of microorganism need
Just work under the conditions of characteristic, thus cause in actual application effect it is unstable.
The existing plant source organic matter of full member biological organic fertilizer, and have animal sources organic matter, while being added to complex microorganism
Therefore microbial inoculum and inorganic fertilizer concentrate organic and inorganic, microorganism fertilizer advantage in one, and overcome organic and inorganic, micro- life
The disadvantage of object fertilizer has the characteristics that full member, and fertilizer efficiency is long and fast, disclosure satisfy that the full nutrition needs of crop, and crop quality is turned up,
It realizes crop yield, increase income purpose.Therefore, complete first biological organic fertilizer has huge application prospect and value.
Currently, the type of complete first biological organic fertilizer in the market is more, but production cost is generally higher, and product standard is not
It is unified, lack a kind of complete first biological organic fertilizer being exclusively used in leaf vegetables.It is therefore desirable to develop a kind of production cost it is low and
Complete first biological organic fertilizer suitable for leaf vegetables plantation.
Invention content
The purpose of the present invention is to provide special complete first biological organic fertilizers of a kind of leaf vegetables and preparation method thereof, which contains
Complex micro organism fungicide, organic fertilizer, inorganic composite fertilizer, synergist, simple production process can make disposable base manure in fertilising
Apply, also can top dressing in the future as needed, meet leaf vegetables growth needs, achieve the effect that volume increase, increase income.
Technical solution of the present invention is as follows:
A kind of special complete first biological organic fertilizer of leaf vegetables, the fertilizer are grouped as by the group of following parts by weight:Composite microbial bacteria
5~15 parts of agent, 50~75 parts of organic fertilizer, 15~40 parts of inorganic composite fertilizer, 0.3~0.8 part of synergist.
Preferably, the special complete first biological organic fertilizer of the leaf vegetables, is grouped as by the group of following parts by weight:Complex microorganism
8~12 parts of microbial inoculum, 55~65 parts of organic fertilizer, 25~30 parts of inorganic composite fertilizer, 0.5~0.7 part of synergist.
It is highly preferred that the special complete first biological organic fertilizer of the leaf vegetables, is grouped as by the group of following parts by weight:Composite microbial
10 parts of object microbial inoculum, 65 parts of organic fertilizer, 24.4 parts of inorganic composite fertilizer, 0.6 part of synergist.
In the present invention, the complex micro organism fungicide by bacillus amyloliquefaciens, Trichoderma harzianum, Rhodopseudomonas palustris,
It is obtained by mixing according to the ratio between living bacteria count 7: 1: 2, the total living bacteria count of complex micro organism fungicide is 10~3,000,000,000 cfu/g, excellent
Selection of land, the total living bacteria count of complex micro organism fungicide is 15~2,500,000,000 cfu/g, it is highly preferred that the composite microbial bacteria
The total living bacteria count of agent is 2,000,000,000 cfu/g.
In the present invention, the organic fertilizer is 45~70 parts of pig manure, 30~55 parts of rice straw, under fermenting agent effect,
Thoroughly decomposed, gained after sieving powder;
Wherein, the microorganism in the fermenting agent is bacillus subtilis, Trichoderma harzianum, and total living bacteria count is 100
~300 hundred million cfu/g, total living bacteria count in the preferably described fermenting agent are 150~25,000,000,000 cfu/g, more preferably it is total effectively
Viable count is 20,000,000,000 cfu/g.
In the present invention, the inorganic composite fertilizer is by 30~45 parts of urea, 30~45 parts of monoammonium phosphate, potassium sulfate 20~35
Part mixture, working process form.
In the present invention, the synergist is mixed by compound sodium nitrophenolate, amine fresh fat (DA-6), humic acid;
Wherein, synergist is preferably as following formula:1~5 part of compound sodium nitrophenolate, 6~12 parts of amine fresh fat, 88~92 parts of humic acid.
The present invention also provides a kind of preparation methods of the special complete first biological organic fertilizer of leaf vegetables, include the following steps:
(1) prepared by complex micro organism fungicide
Wherein, the number of bacillus amyloliquefaciens is ACCC10619, the number of Trichoderma harzianum is ACCC31707, marsh is red
The number of pseudomonad is ACCC10649;
It is prepared by I bacillus amyloliquefaciens microbial inoculums:Aseptically by bacillus amyloliquefaciens strain, respectively by inclined-plane
Activation, shaking table seed culture, fermentation tank seed culture, fermentation tank expand culture, wait for that the gemma rate in zymotic fluid is 90% or more
When fermentation ends, bacillus amyloliquefaciens microbial inoculums then is made by filtering, centrifuging with after spray drying in zymotic fluid;
It is prepared by II Trichoderma harzianum conidia powders:Aseptically by Trichoderma harzianum original strain, it is oblique to pass through eggplant type bottle respectively
After face culture, shaking flask Liquid Culture, fermentor liquid culture, solid fermentation culture production spore, the culture of complete production spore is dry,
Trichoderma harzianum conidia powder is obtained after smashing;
It is prepared by III Rhodopseudomonas palustris bacterium powders:By Rhodopseudomonas palustris strain, aseptically, by inclined-plane
Activation, seed culture, fluid enlargement culture, are waited for after fermentation, and zymotic fluid is carried out centrifugal concentrating, according to proper proportion and load
After body rice husk powder combination drying is prepared into bacterium powder drying, Rhodopseudomonas palustris agent is obtained;
By bacillus amyloliquefaciens, Trichoderma harzianum, Rhodopseudomonas palustris according to the cfu/ of total living bacteria count 10~2,000,000,000
G, the ratio that the ratio between living bacteria count is 7: 1: 2 mix to obtain complex micro organism fungicide;
(2) prepared by organic fertilizer:After mixing by pig manure, rice straw, then in the mixture according to 2~4 ‰ matter
Amount is piled 0.8~1.2 meter of height, adjusts and control moisture 50~60%, fermented using trough composting than addition compost bacterium
Technique measures fermentation temperature daily, and when temperature reaches 60 DEG C, horse back turning ensures 50~60 degree or so of fermentation temperature 10 days
More than, then increase turning number, accelerates moisture evaporation, detection moisture is primary within 2 days or so;When moisture be reduced to 30% hereinafter,
When temperature of charge is room temperature, fermentation ends smash, sieving, obtain powdery organic fertilizer;
(3) prepared by inorganic composite fertilizer:Proportionally after mixing by urea, monoammonium phosphate, potassium sulfate, be sieved to get
Inorganic composite fertilizer;
(4) prepared by synergist:Compound sodium nitrophenolate, DA-6, humic acid are proportionally uniformly mixed to get synergist;
(5) in proportion that the complex micro organism fungicide of step (1)~(4) kind, organic fertilizer, inorganic composite fertilizer, synergist is mixed
It closes uniformly to get the special complete first biological organic fertilizer of leaf vegetables.
The beneficial effects of the present invention are:
The present invention provides a kind of special complete first biological organic of leaf vegetables according to the growth characteristics and nutrient uptake feature of leaf vegetables
Fertilizer and preparation method thereof, after compost bacterium is added in agricultural solid residue (pig manure and rice straw), using slot type high temperature nuclear reactor
Fertilizer technology makes fermentation be brought rapidly up, and maintains 50~60 degree 10 days or more, kills pathogenic microorganism, roundworm egg etc. in compost
Keep its innoxious, and organic materials is decomposed into the small-molecule substance conducive to plant absorption, obtains organic fertilizer.Again by composite microbial
Leaf vegetables dedicated fertilizer is made in object microbial inoculum, inorganic composite fertilizer, synergist after mixing according to a certain percentage.
Fertilizer provided by the invention integrates the advantage of organic and inorganic microorganism fertilizer, synergist, and overcome it is organic,
The shortcomings that inorganic, microorganism fertilizer, has the characteristics that full member, and fertilizer efficiency is long and fast, disclosure satisfy that full nutrition needs of crop, raising
Crop quality realizes leaf vegetables crop volume increase, increases income purpose.Wherein, complex micro organism fungicide can improve soil nutrient, microorganism
The dissolving and release that can promote difficultly soluble nutrient in soil, improve the deliverability of soil nutrient and meanwhile be added microorganism also
The generation of crop soil-borne disease can effectively be prevented.Organic fertilizer has improvement soil, culture fertility, enhances plant resistance, carries
The effect of high crop quality.By the effect for combining microorganism with organic fertilizer, edaphon growth and numerous can be promoted
It grows, increases population diversity, improve enzymatic activity, and accelerate decomposition, the conversion of organic matter in the case where they are acted on, improve organic fat
Effect etc..Inorganic composite fertilizer in the present invention is mainly the easily scarce element of some leaf vegetables good quality and high outputs, organic fertilizer and chemical fertilizer
It mutually promotes, not only contributes to crop absorption, improve the utilization rate of fertilizer, moreover it is possible to adjust soil physico-chemical property and biology
Matter such as improves retention capacity of soil, to further be beneficial to the growth and breeding of microorganism, further promotes fertilizer efficiency.The present invention
In also added synergist and answer DA-6 and nitre phenol sodium, DA-6 is a kind of efficient plant growth substance, is had to multiple kinds of crops aobvious
Work increases production, is degeneration-resistant, is disease-resistant, improving quality effect, and compound sodium nitrophenolate is a kind of efficient plant integrating nutrition, adjusting, diseases prevention
Object growth regulator can accelerate the speed of growth, improve Crops production and quality, when being compounded with full-elementary fertilizer, can release fertilizer
Between antagonism, and make full-elementary fertilizer while being absorbed and used by plants, and compound sodium nitrophenolate all has broad spectrum activity and length with DA-6
Phase usability, high efficiency, nontoxic, nuisanceless, noresidue advantage, therefore can greatly increase fertilizer as leaf vegetables fertilizer synergist
Fertilizer efficiency.The present invention mixes complex micro organism fungicide, organic fertilizer, inorganic composite fertilizer, synergist according to a certain percentage so that specially
Be able to organically combine the effect of various composition in fertilizer, it is synergistic, realize the target of " high yield, high-quality, efficient ".
Specific implementation mode
Reagent described in embodiment and material commercially obtain unless otherwise specified, or can be in conventional manner
It prepares.
Embodiment 1
The special complete first biological organic fertilizer of leaf vegetables contains the raw material of following parts by weight meter:
10 parts of complex micro organism fungicide;
65 parts of organic fertilizer;
24.4 parts of inorganic composite fertilizer;
0.6 part of synergist.
The complex micro organism fungicide is by bacillus amyloliquefaciens, Trichoderma harzianum, Rhodopseudomonas palustris, according to effective living
The ratio between bacterium number 7: 1: 2 is obtained by mixing, total 2,000,000,000 cfu/g of living bacteria count of complex micro organism fungicide.
The organic fertilizer is 60 parts of pig manure, 40 parts of rice straw, in the case where adding the fermenting agent effect of 4 ‰ mass ratioes, hair
Thorough decomposed, gained after sieving the powder of ferment.
The inorganic composite fertilizer by 35 parts of urea, 35 parts of monoammonium phosphate, 30 parts of mixtures of potassium sulfate, be sieved.
The synergist is mixed by following weight meter raw material:3 parts of compound sodium nitrophenolate, 9 parts of DA-6,88 parts of humic acid.
Embodiment 2
The special complete first biological organic fertilizer of leaf vegetables contains the raw material of following parts by weight meter:
5 parts of complex micro organism fungicide;
55 parts of organic fertilizer;
39.6 parts of inorganic composite fertilizer;
0.4 part of synergist.
The complex micro organism fungicide is by bacillus amyloliquefaciens, Trichoderma harzianum, Rhodopseudomonas palustris, according to effective living
The ratio between bacterium number 7: 1: 2 is obtained by mixing, total 1,000,000,000 cfu/g of living bacteria count of complex micro organism fungicide.
The organic fertilizer is 45 parts of pig manure, 55 parts of rice straw, in the case where adding the fermenting agent effect of 6 ‰ mass ratioes, fermentation
Thoroughly decomposed, gained after sieving powder.
The complex micro organism fungicide is by bacillus amyloliquefaciens, Trichoderma harzianum, Rhodopseudomonas palustris, according to effective living
The ratio between bacterium number 7: 1: 2 is obtained by mixing, total 2,500,000,000 cfu/g of living bacteria count of complex micro organism fungicide.
The inorganic composite fertilizer by 35 parts of urea, 35 parts of monoammonium phosphate, 30 parts of mixtures of potassium sulfate, be sieved.
The synergist is mixed by following weight meter raw material:2 parts of compound sodium nitrophenolate, DA-68 parts, 92 parts of humic acid.
Embodiment 3
The special complete first biological organic fertilizer of leaf vegetables contains the raw material of following parts by weight meter:
8 parts of complex micro organism fungicide;
75 parts of organic fertilizer;
16.7 parts of inorganic composite fertilizer;
0.3 part of synergist.
The complex micro organism fungicide is by bacillus amyloliquefaciens, Trichoderma harzianum, Rhodopseudomonas palustris, according to effective living
The ratio between bacterium number 7: 1: 2 is obtained by mixing, total 2,500,000,000 cfu/g of living bacteria count of complex micro organism fungicide.
The organic fertilizer is 70 parts of pig manure, 30 parts of rice straw, in the case where adding the fermenting agent effect of 4 ‰ mass ratioes, hair
Thorough decomposed, gained after sieving the powder of ferment.
The inorganic composite fertilizer by 45 parts of urea, 35 parts of monoammonium phosphate, 20 parts of mixtures of potassium sulfate, be sieved.
The synergist is mixed by following weight meter raw material:5 parts of compound sodium nitrophenolate, 12 parts of DA-6,83 parts of humic acid.
Test example 1
Application effect of the special complete first biological organic fertilizer of leaf vegetables on Brassica rapa L
1. test objective:Verify the effect that the special complete first biological organic fertilizer of leaf vegetables promotes leaf vegetables yield.
2. subjects and kind:" golden product summer song " Brassica rapa L
3. testing site:Positioned at the liuyang hunan city towns Bei Sheng proving ground
4. experimental design
6 groups of processing are arranged in 4.1 experiments altogether, each handle 3 cells, are 90 square metres per plot area, minizone is set
Aisle, surrounding setting protection row, 0.5kg/ mus of application rate.Hillslope processes and check plot, are randomly assigned, and mark.Experiment
Processing is as follows:
Test group (TR):The complete first biological organic fertilizer 120kg prepared according to the fertilising embodiment of the present invention 1 per acre.
Control group (CK1):It does not apply fertilizer.
Control group (CK2):Using chemical fertilizer (urea and compound fertilizer, the use suggested according to producer of addition common fertilizer
Amount application).
Control group (CK3):120kg composite fertilizers 1, the full member biology that the formula of composite fertilizer 1 is prepared according to embodiment 1 are applied per acre
Organic fertilizer formula, but it is added without the formula of 65 parts of organic fertilizers, it compounds.
Control group (CK4):120kg composite fertilizers 2, the full member biology that the formula of composite fertilizer 2 is prepared according to embodiment 1 are applied per acre
Organic fertilizer formula, but it is added without the formula of 24.4 parts of inorganic composite fertilizers, it compounds.
Control group (CK5):120kg composite fertilizers 3, the full member biology that the formula of composite fertilizer 3 is prepared according to embodiment 1 are applied per acre
Organic fertilizer formula, but it is added without the formula of 0.6 part of synergist, it compounds.
Above 6 groups of processing make base manure application when being site preparation bedding prior to seeding.
4.2 experiments are implemented
It is executed according to 4.1, Routine Management.Periodic measurement Brassica rapa L growth index harvests for 50 days after field planting.
5 test results
Influence of 1 different disposal of table to Brassica rapa L biological character and structure of output
As seen from the results in Table 1, applied the plant height of the Brassica rapa L of complete first biological organic fertilizer of the present invention, single-strain blade number,
Vc contents, yield are higher than do not apply fertilizer control (CK1) and control group (CK2-CK5).The complete first biology that applied the present invention has
The Brassica rapa L of machine fertilizer, yield reach 2568.5Kg/ mus, and compared to the 1813.7Kg/ mus of (CK1) of not applying fertilizer, amount of increase in production reaches
41.6%;Compared to the 2053.7Kg/ mus of application common fertilizer (CK2), amount of increase in production is up to 25.1%.It can also be seen that by table 1
By working in coordination between complex micro organism fungicide, organic fertilizer, inorganic fertilizer, synergist, there is cooperative synergism effect, either
Plant height, single-strain blade number, Vc contents or per mu yield, indices have reached each group highest.The test result shows to apply
Complete first biological organic fertilizer provided by the present invention can significantly improve the yield and quality of leaf vegetables crop.
The above various embodiments is used to illustrate the present invention, but is not limited to the scope of the present invention.Without departing substantially from the present invention
In the case of spirit and essence, to modifications or substitutions made by the method for the present invention, step or condition, the protection of the present invention is belonged to
Range.