CN103011917A - Multi-resistant bio-organic fertilizer and preparation method thereof - Google Patents
Multi-resistant bio-organic fertilizer and preparation method thereof Download PDFInfo
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- CN103011917A CN103011917A CN2011102869499A CN201110286949A CN103011917A CN 103011917 A CN103011917 A CN 103011917A CN 2011102869499 A CN2011102869499 A CN 2011102869499A CN 201110286949 A CN201110286949 A CN 201110286949A CN 103011917 A CN103011917 A CN 103011917A
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Abstract
The invention relates to a bio-organic fertilizer, especially to a multi-resistant bio-organic fertilizer and a preparation method thereof. The multi-resistant bio-organic fertilizer is prepared by mixing and fermenting mushroom residue, humic acid, bovine blood polypeptide and high-efficiency microbial agents with a weight part ratio of 1 : 2 : 1 : 2. The organic fertilizer provided by the invention has comprehensive and lasting nutrients, can continuously transform soil nutrients, and has a fertilizer efficiency as long as 120 days; meanwhile, the organic fertilizer contains a lot of beneficial microbes, and thus can secrete biologically-active substance, improve soil structure, solve a problem of soil hardening, and recover soil self-production capacity; and is beneficial for exchanging of water, fertilizer, gas and heat in the soil, can reduce pesticide residues, and improves living environment of the plants.
Description
Technical field
The present invention relates to biological organic fertilizer, specifically a kind of many antibionts fertilizer and preparation method thereof.
Background technology
Present stage, aquaculture exceeded the several times of the highest standard of World Health Organization's regulation to the harm of HUMAN HEALTH by seriously polluted to soil and environment of the fertilizer of Chemical Composition, and for this reason, the scientist of countries in the world is for improving the global environment effort.
The Ministry of Agriculture of the People's Republic of China, MOA advocates production and the application of development biological organic fertilizer energetically in the meeting of " ten large practical techniques are promoted in outstanding ten major tasks ", improve environment and guarantee that the safety of food has become the vital task of whole society's development.
Organic fertilizer is national key new product, product is that fertile medicine economic benefits and social benefits are arranged, comprehensively and nutrition, the outstanding feature of environmental protection. collection is controlled the efficacy of bacterium, the synergy of the quick-acting of mineral manure, trace element and plant physiology material with bacterium, quaternity, widely applicable. the peculiar effect of the fertile medicine economic benefits and social benefits of obvious effect of increasing production, particularly product fills the domestic gaps, being in world lead level, is the optimum fertilizer of efficient green agricultural.
Summary of the invention
The object of the invention is to provide a kind of many antibionts fertilizer and preparation method thereof.
For achieving the above object, the technical solution used in the present invention is:
A kind of many antibionts fertilizer comprises nitrogen, phosphorus, potassium, and many antibionts fertilizer is formed by bacterium chaff, humic acids, ox blood polypeptide and the by weight mixed fermentation of high-effective microorganism microbial inoculum; Count by weight bacterium chaff: humic acids: ox blood polypeptide: high-effective microorganism microbial inoculum=1: 2: 1: 2
Described bacterium chaff is the waste residue behind the main raw material culturing edible fungus from production mushroom, cotton seed hulls, wood sawdust, straw, corn cob and multiple kinds of crops stalk, industrial waste vinasse; Contain humic acids total amount 〉=40% in the humic acids, ph value＜7.0, humic acids is 50 orders.
Described ox blood polypeptide prepares as follows:
1) get fresh virus-free ox blood centrifuging and taking supernatant serum, then regulate pH3.4, stand-by;
2) add composite hydrolytic enzyme in the serum after will regulating, in 45-40 ℃ of lower insulation hydrolysis 8-6h, will be hydrolyzed rear serum and be adjusted to neutrality; (this is right, and amount of hydrolysis is different under different temperature)
3) be heated to 85-70 ℃ with being adjusted to neutral serum after the said hydrolyzed, keep 30-25min, then be cooled to room temperature, the centrifuging and taking supernatant liquor, through the ultra-filtration membrane ultrafiltration, gained filtrate is the ox blood polypeptide.
Centrifugal condition in described step 1 and the step 3 is 5000-4000rpm, 30-25min.Described step 1) add-on of composite hydrolytic enzyme is the 0.2-0.1% (W/V) of serum in the serum.Described step 3) centrifugal rear supernatant liquor is through the ultrafiltration of 10k ultra-filtration membrane.
The preparation method of many antibionts fertilizer: with ox blood polypeptide and high-effective microorganism microbial inoculum mix and blend fermentation 7-10 days, regulate water content and bacterium chaff and humic acids sneaked into to stir to the 25-20% and namely obtain many antibionts fertilizer.
Described bacterium chaff is the waste residue behind the main raw material culturing edible fungus from production mushroom, cotton seed hulls, wood sawdust, straw, corn cob and multiple kinds of crops stalk, industrial waste vinasse; Contain humic acids total amount 〉=40% in the humic acids, ph value＜7.0, humic acids is 50 orders.
Described ox blood polypeptide prepares as follows: 1) gets fresh virus-free ox blood centrifuging and taking supernatant serum, then regulates pH3.4-2, and stand-by; 2) add composite hydrolytic enzyme in the serum after will regulating, in 45-40 ℃ of lower insulation hydrolysis 8-6h, will be hydrolyzed rear serum and be adjusted to neutrality; 3) be heated to 85-70 ℃ with being adjusted to neutral serum after the said hydrolyzed, keep 30-25min, then be cooled to room temperature, the centrifuging and taking supernatant liquor, through the ultra-filtration membrane ultrafiltration, gained filtrate is the ox blood polypeptide.
Described gained biological organic fertilizer also can add other multiple-microorganism preparations to be mixed by drying, but makes the fertilizer water content reach granulation below 20% or pack obtains finished product.
When adopting fertilizer of the present invention as base manure: the cash crop such as vegetables are used 80-150 kg/acre fertilizer of the present invention, grain is used 80-100 kg/acre fertilizer of the present invention, tea tree is used 80-100 kg/acre fertilizer of the present invention, fruit tree use the 1-3 kilogram/fertilizer of the present invention, the lawn use the 0.5-1 kilogram/square metre fertilizer of the present invention; Then suitably impose as required, when making base manure simultaneously, the crop in 100 days vegetative period need not to topdress.
The present invention has advantages of:
1. many antibionts of the present invention fertilizer has fertilizer, medicine economic benefits and social benefits, full nutrition, three large characteristics of environmental protection, wherein probiotics is greater than 20,000,000,000/kilogram in the fertilizer, and total nutrient is greater than 12%, and middle trace element is greater than 5%, organic greater than 30%, humic acid is greater than 15%, and amino acid is rich in multivitamin greater than 5%, microbiotic, tethelin and high-efficiency activated enzyme.
2. many antibionts of the present invention fertilizer has preventive and therapeutic effect to blight, verticillium, damping-off, root rot, bacterial wilt, samping off, anthrax, sooty mold, climing post disease, gray mold, black root, soft rot, club root, leaf blight, yellowtop, epidemic disease etc.
3. fertilizer nutrient comprehensive and lasting of the present invention, can transform continuously soil nutrient, fertilizer efficiency reaches 120 days, contains simultaneously a large amount of beneficial microorganisms, can secrete biologically active substance, the improvement Soil structure, solve the soil compaction problem, recover soil oneself throughput, and be conducive to water, fertilizer, gas, heat and in soil, exchange, reduce farming residual, improve the plant life environment.
Embodiment
Embodiment 1
Many antibionts fertilizer, many antibionts fertilizer is formed by bacterium chaff, humic acids, ox blood polypeptide and the by weight mixed fermentation of high-effective microorganism microbial inoculum; Count by weight bacterium chaff: humic acids: ox blood polypeptide: high-effective microorganism microbial inoculum=1: 2: 1: 2.
Above-mentioned materials source is: its characteristics of high-effective microorganism microbial inoculum: firepower is strong, high temperature resistant, good stress resistance, in soil the survival time long microbial strains, contain various high-efficiency activated microorganisms and the biologically active substances such as biological and ecological methods to prevent plant disease, pests, and erosion, phosphorus decomposing, potassium decomposing, fixed nitrogen, thereby solved general boron, zinc, magnesium be manured into soil easily be fixed afterwards, drawback that utilization ratio is low, for crop provides good micro-ecological environment, improve soil, improve quality of agricultural product, strengthen farm crop resistance, promote healthy growth of crops.This product and various fertilizer are joined and are executed, can promote microorganism and the soil beneficial microorganism allocated into to breed, effectively prevent the loss, volatilization, and nitrated of nitrogen, reduce phosphorus potassium fixation and ineffective treatment, greatly improved the utilization ratio (available from the special bold and unconstrained biological company limited in Jiangsu) of fertilizer.
Contain total humic acid amount 〉=40% among the humic acids HA, derive from brown coal (weathered coal), ph value＜7.0. bacterium chaff is the waste residue behind the main raw material culturing edible fungus from production mushroom, cotton seed hulls, wood sawdust, straw, corn cob and multiple kinds of crops stalk, industrial waste vinasse.
The preparation of many antibionts fertilizer: water content the ox blood polypeptide below 60% and high-effective microorganism microbial inoculum mix and blend fermentation 7-10 days, is regulated after the water content to 25% bacterium chaff and humic acids sneaked into to stir namely obtaining many antibionts fertilizer.
Bacterium chaff, humic acids, ox blood polypeptide and high-effective microorganism microbial inoculum are counted by weight, bacterium chaff: humic acids: the ox blood polypeptide: high-effective microorganism microbial inoculum=1: 2: 1: 2.
The preparation of ox blood polypeptide:
1) get fresh ox arterial blood and do virus and detect, inactivation treatment then does after the inactivation treatment for the second time that virus detects, and guarantees in the blood virus-freely by 100 purpose nylon net filters, collects 0 ℃ of preservation of filtrate, and is stand-by.
2) with above-mentioned fresh, virus-free ox blood with 5000r/min, centrifugal 30min collects supernatant liquor and then regulates serum pH to 3.4 with hydrochloric acid, then is heated to 45 ℃;
3) 0.2%W/V by the serum total amount adds composite hydrolytic enzyme (a kind of complex enzyme preparation special for the animal proteinum hydrolysis of composite hydrolytic enzyme to regulating in the rear serum under agitation condition, it mainly is comprised of protein incision enzyme, excision enzyme and food flavor enzyme etc., cutting off the peptide chain of protein interior and excision enzyme from the centre by restriction endonuclease cuts off from the end of polypeptide chain and discharges amino acid, and food flavor enzyme plays optimization function to bitter taste and the local flavor that is hydrolyzed), in 45 ℃ of lower insulation hydrolysis 8h, serum is regulated pH value to 6.5 after will being hydrolyzed;
4) serum that is adjusted to pH value to 6.5 after the said hydrolyzed is passed into be steam heated to 85 ℃ and keep 30min, pass into the distilled water cool to room temperature behind the steam off at once; With hydrolyzed solution 5000r/min, centrifuging and taking supernatant under the 30min condition, again through the ultra-filtration membrane ultrafiltration of 10K, filtrate namely obtains the ox blood polypeptide, is dried to make its water content be lower than 60%, and is stand-by after supernatant being filtered again.
Application examples: with the fertilizer of above-described embodiment preparation, amount of application is 40 kg/acres within the vegetative period of paddy rice, disposable applying.Conventional fertile the processing used urea 19.34kg/ mu, double superhosphate 9.6kg/ mu, Repone K 8kg/ mu.Special fertilizer can improve number of productive ear, grain number per spike, thousand seed weight and plant height, increases by 8%, 20.8%, 0.5% and 9% than conventional fertilizer application than respectively, can improve output 10.9%, sees following table 1 for details.
The stable paddy rice-dedicated fertilizer test-results of table 1 table
| Process | Number of productive ear | Plant height | Spike length | Grain number per spike | Setting percentage | Thousand seed weight | Output | Stimulation ratio |
| (10 4/ mu) | (cm) | (cm) | (％) | (g) | (kg/ mu) | (％) | ||
| Conventional fertile | 37.28 | 78.83 | 14.75 | 76.12 | 95.97 | 25.88 | 599.80 | -- |
| Special fertilizer | 40.27 | 85.93 | 15.91 | 91.93 | 93.75 | 26.02 | 665.19 | 10.90 |
Claims (10)
1. antibiont fertilizer more than a kind comprises nitrogen, phosphorus, potassium, it is characterized in that: many antibionts fertilizer is formed by bacterium chaff, humic acids, ox blood polypeptide and the by weight mixed fermentation of high-effective microorganism microbial inoculum; Count by weight bacterium chaff: humic acids: ox blood polypeptide: high-effective microorganism microbial inoculum=1: 2: 1: 2.
2. by many antibionts fertilizer claimed in claim 1, it is characterized in that: described bacterium chaff is the waste residue behind the main raw material culturing edible fungus from production mushroom, cotton seed hulls, wood sawdust, straw, corn cob and multiple kinds of crops stalk, industrial waste vinasse; Contain humic acids total amount 〉=40% in the humic acids, ph value＜7.0, humic acids is 50 orders.
3. by many antibionts fertilizer claimed in claim 1, it is characterized in that: described ox blood polypeptide prepares as follows:
1) get fresh virus-free ox blood centrifuging and taking supernatant serum, then regulate pH3.4, stand-by; 2) add composite hydrolytic enzyme in the serum after will regulating, in 45-40 ℃ of lower insulation hydrolysis 8-6h, will be hydrolyzed rear serum and be adjusted to neutrality; 3) be heated to 85-70 ℃ with being adjusted to neutral serum after the said hydrolyzed, keep 30-25min, then be cooled to room temperature, the centrifuging and taking supernatant liquor, through the ultra-filtration membrane ultrafiltration, gained filtrate is the ox blood polypeptide.
4. by many antibionts fertilizer claimed in claim 3, it is characterized in that: the centrifugal condition in described step 1 and the step 3 is 5000-4000rpm, 30-25min.
5. by many antibionts fertilizer claimed in claim 3, it is characterized in that: described step 1) add-on of composite hydrolytic enzyme is the 0.2-0.1% (W/V) of serum in the serum.
6. by many antibionts fertilizer claimed in claim 3, it is characterized in that: described step 3) centrifugal rear supernatant liquor is through the ultrafiltration of 10k ultra-filtration membrane.
7. the preparation method of many antibionts fertilizer claimed in claim 1, it is characterized in that: with ox blood polypeptide and high-effective microorganism microbial inoculum mix and blend fermentation 7-10 days, regulate water content and bacterium chaff and humic acids sneaked into to stir to the 25-20% and namely obtain many antibionts fertilizer.
8. by the preparation method of many antibionts fertilizer claimed in claim 7, it is characterized in that: described bacterium chaff is the waste residue behind the main raw material culturing edible fungus from production mushroom, cotton seed hulls, wood sawdust, straw, corn cob and multiple kinds of crops stalk, industrial waste vinasse; Contain humic acids total amount 〉=40% in the humic acids, ph value＜7.0, humic acids is 50 orders.
9. by the preparation method of many antibionts fertilizer claimed in claim 7, it is characterized in that: described ox blood polypeptide prepares as follows: 1) get fresh virus-free ox blood centrifuging and taking supernatant serum, then regulate pH3.4-2, and stand-by; 2) add composite hydrolytic enzyme in the serum after will regulating, in 45-40 ℃ of lower insulation hydrolysis 8-6h, will be hydrolyzed rear serum and be adjusted to neutrality; 3) be heated to 85-70 ℃ with being adjusted to neutral serum after the said hydrolyzed, keep 30-25min, then be cooled to room temperature, the centrifuging and taking supernatant liquor, through the ultra-filtration membrane ultrafiltration, gained filtrate is the ox blood polypeptide.
10. press the preparation method of many antibionts fertilizer claimed in claim 7; it is characterized in that: described gained biological organic fertilizer also can add other multiple-microorganism preparations to be mixed by drying, but makes the fertilizer water content reach granulation below 20% or pack obtains finished product.
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| CN2011102869499A CN103011917A (en) | 2011-09-23 | 2011-09-23 | Multi-resistant bio-organic fertilizer and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103396228A (en) * | 2013-07-31 | 2013-11-20 | 常熟市常福有机复合肥有限公司 | Biological active peptide organic fertilizer |
| CN104003769A (en) * | 2014-06-05 | 2014-08-27 | 湖北大学 | Duck blood peptide preparation method and application of duck blood peptide in plantation of duck blood tea |
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| CN1129683A (en) * | 1995-04-14 | 1996-08-28 | 北京兆元技术发展公司 | Multiple component organic composite liquid fertilizer and preparation thereof |
| US20020053229A1 (en) * | 2000-06-01 | 2002-05-09 | Amir Varshovi | Organic-based fertilizer |
| CN1594232A (en) * | 2004-07-01 | 2005-03-16 | 李长江 | Composite amino acid ecological nutritious agent |
| CN1843376A (en) * | 2006-05-15 | 2006-10-11 | 锦州奥鸿药业有限责任公司 | Method for extracting and purifying calf serum protein-removing extract |
| CN101781149A (en) * | 2010-02-08 | 2010-07-21 | 张钧 | Bio-organic compound fertilizer |
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| CN1112537A (en) * | 1994-05-21 | 1995-11-29 | 北京兆元技术发展公司 | Processing method of compound organic liquid spray fertilizer |
| CN1129683A (en) * | 1995-04-14 | 1996-08-28 | 北京兆元技术发展公司 | Multiple component organic composite liquid fertilizer and preparation thereof |
| US20020053229A1 (en) * | 2000-06-01 | 2002-05-09 | Amir Varshovi | Organic-based fertilizer |
| CN1594232A (en) * | 2004-07-01 | 2005-03-16 | 李长江 | Composite amino acid ecological nutritious agent |
| CN1843376A (en) * | 2006-05-15 | 2006-10-11 | 锦州奥鸿药业有限责任公司 | Method for extracting and purifying calf serum protein-removing extract |
| CN101781149A (en) * | 2010-02-08 | 2010-07-21 | 张钧 | Bio-organic compound fertilizer |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103396228A (en) * | 2013-07-31 | 2013-11-20 | 常熟市常福有机复合肥有限公司 | Biological active peptide organic fertilizer |
| CN104003769A (en) * | 2014-06-05 | 2014-08-27 | 湖北大学 | Duck blood peptide preparation method and application of duck blood peptide in plantation of duck blood tea |
| CN104003769B (en) * | 2014-06-05 | 2016-05-04 | 湖北大学 | A kind of preparation method of duck hemepeptide and duck hemepeptide are in the application of plantation duck blood tea |
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Application publication date: 20130403 |