CN105646085A - Liquid microbial organic fertilizer - Google Patents
Liquid microbial organic fertilizer Download PDFInfo
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- CN105646085A CN105646085A CN201610116361.1A CN201610116361A CN105646085A CN 105646085 A CN105646085 A CN 105646085A CN 201610116361 A CN201610116361 A CN 201610116361A CN 105646085 A CN105646085 A CN 105646085A
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- 239000007788 liquid Substances 0.000 title claims abstract description 58
- 230000000813 microbial effect Effects 0.000 title claims abstract description 27
- 239000003895 organic fertilizer Substances 0.000 title claims abstract description 22
- 239000003337 fertilizer Substances 0.000 claims abstract description 60
- 239000011573 trace mineral Substances 0.000 claims abstract description 33
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 33
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 25
- 150000003839 salts Chemical class 0.000 claims abstract description 18
- 239000013522 chelant Substances 0.000 claims abstract description 15
- 239000000203 mixture Substances 0.000 claims abstract description 14
- 239000002921 fermentation waste Substances 0.000 claims abstract description 8
- 239000002699 waste material Substances 0.000 claims description 35
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 20
- 230000032823 cell division Effects 0.000 claims description 20
- 239000003002 pH adjusting agent Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 239000012530 fluid Substances 0.000 claims description 19
- 229910021538 borax Inorganic materials 0.000 claims description 17
- 239000004328 sodium tetraborate Substances 0.000 claims description 17
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 17
- YTSDVWYUJXKXCC-UHFFFAOYSA-L zinc;2-[2-[carboxylatomethyl(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetate Chemical compound [Zn+2].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O YTSDVWYUJXKXCC-UHFFFAOYSA-L 0.000 claims description 15
- 239000011259 mixed solution Substances 0.000 claims description 11
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 10
- 239000004202 carbamide Substances 0.000 claims description 10
- 235000013877 carbamide Nutrition 0.000 claims description 10
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 10
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 10
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 10
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 claims description 10
- 229910052939 potassium sulfate Inorganic materials 0.000 claims description 10
- 235000011151 potassium sulphates Nutrition 0.000 claims description 10
- 241000193755 Bacillus cereus Species 0.000 claims description 9
- 239000000084 colloidal system Substances 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 241000194107 Bacillus megaterium Species 0.000 claims description 8
- 238000010438 heat treatment Methods 0.000 claims description 8
- 239000000375 suspending agent Substances 0.000 claims description 8
- 244000063299 Bacillus subtilis Species 0.000 claims description 7
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 7
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- -1 count in mass ratio Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 5
- 239000005416 organic matter Substances 0.000 claims description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 2
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 2
- 150000001413 amino acids Chemical class 0.000 claims description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 2
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 2
- 159000000007 calcium salts Chemical class 0.000 claims description 2
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 2
- 229940099596 manganese sulfate Drugs 0.000 claims description 2
- 239000011702 manganese sulphate Substances 0.000 claims description 2
- 235000007079 manganese sulphate Nutrition 0.000 claims description 2
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 2
- 229910017604 nitric acid Inorganic materials 0.000 claims description 2
- 239000001117 sulphuric acid Substances 0.000 claims description 2
- 235000011149 sulphuric acid Nutrition 0.000 claims description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 2
- 229960001763 zinc sulfate Drugs 0.000 claims description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 2
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 16
- 239000002994 raw material Substances 0.000 abstract description 10
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 230000004083 survival effect Effects 0.000 abstract description 4
- 235000016709 nutrition Nutrition 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 230000001580 bacterial effect Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 235000013379 molasses Nutrition 0.000 description 18
- 244000005700 microbiome Species 0.000 description 16
- 239000000376 reactant Substances 0.000 description 15
- 239000011790 ferrous sulphate Substances 0.000 description 14
- 235000003891 ferrous sulphate Nutrition 0.000 description 14
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 14
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 13
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 11
- 235000010413 sodium alginate Nutrition 0.000 description 11
- 229940005550 sodium alginate Drugs 0.000 description 11
- 239000000661 sodium alginate Substances 0.000 description 11
- 230000001105 regulatory effect Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 8
- 235000015097 nutrients Nutrition 0.000 description 6
- 210000003608 fece Anatomy 0.000 description 5
- 239000010871 livestock manure Substances 0.000 description 4
- 241000233866 Fungi Species 0.000 description 3
- 244000068988 Glycine max Species 0.000 description 3
- 235000010469 Glycine max Nutrition 0.000 description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000019355 sepiolite Nutrition 0.000 description 3
- 229910052624 sepiolite Inorganic materials 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000003247 decreasing effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 1
- OORIKNJWZHTXDC-UHFFFAOYSA-N CCCC(CC)C(CC)(C(=O)OCC)N Chemical compound CCCC(CC)C(CC)(C(=O)OCC)N OORIKNJWZHTXDC-UHFFFAOYSA-N 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 238000005056 compaction Methods 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- ONCZQWJXONKSMM-UHFFFAOYSA-N dialuminum;disodium;oxygen(2-);silicon(4+);hydrate Chemical compound O.[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[Na+].[Na+].[Al+3].[Al+3].[Si+4].[Si+4].[Si+4].[Si+4] ONCZQWJXONKSMM-UHFFFAOYSA-N 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229960001781 ferrous sulfate Drugs 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 238000009655 industrial fermentation Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 229910001410 inorganic ion Inorganic materials 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 150000002823 nitrates Chemical class 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 229940080314 sodium bentonite Drugs 0.000 description 1
- 229910000280 sodium bentonite Inorganic materials 0.000 description 1
- 239000004016 soil organic matter Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/27—Dispersions, e.g. suspensions or emulsions
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention provides a liquid microbial organic fertilizer, which comprises a mixture containing a chelate, a microbial inoculum and a pH regulator, wherein the mixture containing the chelate comprises the following components in percentage by mass: bacterial agent: and (3) pH regulator (19-41): 5-15: 0.5-2, wherein the pH value of the liquid microbial organic fertilizer is 5.5-6.5, the mixture containing the chelate comprises medium and trace element salts and fermentation waste liquid, and the medium and trace element salts are calculated according to the mass ratio: and (3) setting the fermentation waste liquid as 4-11: 15-30, the liquid microbial organic fertilizer takes the fermentation waste liquid as a strain carrier, has low cost, safe and pollution-free raw materials and high strain survival rate, can prolong the quality guarantee period of the fertilizer, and is a liquid microbial fertilizer which has comprehensive nutrition, a large number of effective viable bacteria, a long quality guarantee period, stable fertilizer efficiency and good environmental protection.
Description
Technical field
The present invention relates to fertilizer field, be specifically related to a kind of liquid microbial organic fertilizer.
Background technology
At present, China is in agricultural production, and some areas land management is improper, and soil organic matter content reduces year by year, causes plant nutrient substance seriously uneven, soil compaction and catabiosis, seriously governs the development of China's agricultural.
Microbial manure is to make crop obtain a kind of goods of specific fertilizer effect with the vital movement of microorganism, is the one using fertilizer in agricultural production. It has the history of nearly 50 years in China. Its effect is mainly conditioning soil; Reduce fertilizer application amount; Improve quality of agricultural product; Strengthen crop resistance and resistance; Strengthen the crop integrated campaign ability to poor environment simultaneously. It has been experienced that, ecological organic fertilier can make nitrate salts content of vegetable on average reduce by 48.3��87.7%, nitrogen, phosphorus, potassium content raising 5��20% etc. Microbial manure, to people, animal, Environmental security, nontoxic, is a kind of environment-friendly type fertilizer.
Primary track occasion etc. disclose a kind of method (CN104529626A) of liquid microbe fertilizer, adopt feces of livestock and poultry, Fructus Hordei Germinatus oligose, molasses etc. to do raw material, but fertilizer content is low, and cost is high. Industrial fermentation waste water is rich in a large amount of water-soluble organic matters, and especially molasses containing waste water, the content of organic matter is higher than 40%, and total nutrient accounts for 6%. These waste water are unprocessed is directly discharged into water body, causes the destruction of environmental pollution and ecosystem while wasting resource.
The preparation method of existing microbial manure is to make carrier with raw materials such as pig manure, paddy skin, filter mud of sugarcanes, is added to beneficial microbe and makes. The key problem of microorganism ubiquity the following aspects that this method is produced: (1) effectively bacterium number is very little; (2) shelf-life is short; (3) available nutrient is single.
Summary of the invention
For solving the problems referred to above, the present invention provides a kind of liquid microbe fertilizer, the fermented waste fluid utilizing low cost does microbe carrier and prepares liquid microbe fertilizer, is the liquid microbe fertilizer that comprehensive nutrition, living bacteria count are many, long shelf-life, fertilizer efficiency stable and the feature of environmental protection is good.
A kind of liquid microbial organic fertilizer, including the mixture containing chelate, microbial inoculum, pH adjusting agent, count in mass ratio, mixture containing chelate: microbial inoculum: pH adjusting agent=19��41:5��15:0.5��2, the pH value of described liquid microbial organic fertilizer is 5.5��6.5, the described mixture containing chelate includes medium trace element salt and fermented waste fluid, count in mass ratio, medium trace element salt: fermented waste fluid=4��11:15��30, with fermented waste fluid for strain carrier, cost is low, materials safety is pollution-free, strain survival rate is high, the fertilizer shelf-life can be extended.
Preferably, the described mixture containing chelate also includes inorganic fertilizer, suspending agent, counts in mass ratio, medium trace element salt: fermented waste fluid: inorganic fertilizer: suspending agent=4��11:15��30:25��50:0.1��0.5.
Preferably, the preparation method of the described mixture containing chelate is as follows:
(1) inorganic fertilizer is mixed, add water and stir and grind, form mixed solution;
(2) in mixed solution, medium trace element salt and suspending agent are added;
(3) heating is to 50��100 DEG C, adds fermented waste fluid, reacts 0.5��2 hour.
Step (1) adds the amount of water for count in mass ratio, inorganic fertilizer: water=25��50:10��15.
Preferably, described inorganic fertilizer is two or more in carbamide, ammonium nitrate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium sulfate, ammonium sulfate.
Preferably, described inorganic fertilizer is counted in mass ratio, carbamide: ammonium nitrate: potassium dihydrogen phosphate: potassium sulfate=5��15:5��10:10��15:5��10.
Preferably, described suspending agent is conventional suspending agent in agricultural or fertilizer field, for one or more in sodium alginate, meerschaum, kieselguhr, sodium bentonite or active hargil;
Preferably, possibly together with the basic element of cell division in described liquid microbial organic fertilizer, by the mass ratio of strain, the addition of the described basic element of cell division is, microbial inoculum: the basic element of cell division=5��15:0.01��0.03.
Preferably, possibly together with synergist in described liquid microbial organic fertilizer, described synergist is one or more in diethyl aminoethyl hexanoate, naphthalene acetic acid, heteroauxing, polymerization glutamic acid.
Preferably, the strain in described microbial inoculum is one or more in bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering, colloid bacillus cereus, described microbial inoculum viable count is 20,000,000��200,000,000/gram.
Preferably, described pH adjusting agent is one or more in potassium hydroxide, sodium hydroxide, concentrated sulphuric acid, concentrated nitric acid.
Preferably, described medium trace element salt includes one or more in Borax, EDTA-zinc, zinc sulfate, ferrous sulfate, manganese sulfate, calcium salt, mantoquita.
Preferably, by weight, described Borax: trace element salt=2��4:2��7, it is preferred that the ferrous sulfate in described trace element salt: EDTA-zinc=1��3:1��4.
Preferably, described fermented waste fluid is molasses alcohol fermentation waste liquid and/or amino acid fermentation waste liquid, and the quality of organic matter content in described fermented waste fluid is 25%��60%.
Beneficial effects of the present invention:
(1) the medium trace element salt in the present invention and fermented waste fluid are easier to chelating under preparation method of the present invention, and the mixture containing chelate obtained is easier to utilization absorbed by crops;
(2) after the medium trace element salt in the present invention is chelated, decrease the inorganic ion injury to strain, make effective bacterium number of strain increase;
(3) pH value regulated described in the liquid microbial organic fertilizer of the present invention is 5.5��6.5, and within the scope of this pH, strain preferably is survived, and extends the shelf life;
(4) product nutrient of the present invention is complete and content is high, dispersion effect is good, and microorganism fungus kind survival rate is high, and this fertilizer is the comprehensive nutrient needed for plant growing offer rich in microorganism fungus kind and organic mass-energy, utilization rate and the crop quality of fertilizer and environmental protection and public nuisance free can be improved.
Detailed description of the invention
Below the specific embodiment of the present invention is described further:
Embodiment 1
According to the mass fraction, proportioning raw materials: microbial inoculum 10 parts (bacillus subtilis 3 parts, bacillus megaterium 2 parts, bacillusmusilaginosiengineering 2 parts, colloid bacillus cereus 3 parts, living bacteria count 100,000,000/gram), molasses fermented waste liquid 25 parts (being 60% containing organic mass percent), 37 parts of inorganic fertilizer (10 parts of carbamide, ammonium nitrate 8 parts, potassium dihydrogen phosphate 12 parts, potassium sulfate 7 parts), medium trace element 7.5 parts (Borax 3 parts, 2 parts of ferrous sulfate, 2.5 parts of EDTA-zinc), sodium alginate 0.2 part, the basic element of cell division 0.02 part, pH adjusting agent 1.25 parts, 23 parts of water.
Step:
(1) inorganic fertilizer is mixed, add water and stir and grind, form solution;
(2) in mixed solution, Borax, ferrous sulfate, EDTA-zinc, sodium alginate it are sequentially added into;
(3) heating is to 75 DEG C, adds molasses fermented waste liquid, reacts 1.5 hours;
(4) reactant liquor is down to room temperature;
(5) adding pH adjusting agent, regulating reactant liquor pH is 6, is slowly added to microbial inoculum, the basic element of cell division, obtains liquid microbial organic fertilizer A.
Comparative example 1
According to the mass fraction, comparative example 1 uses the molasses fermented waste liquid of 5 parts, proportioning raw materials: microbial inoculum 10 parts (bacillus subtilis 3 parts, bacillus megaterium 2 parts, bacillusmusilaginosiengineering 2 parts, colloid bacillus cereus 3 parts, living bacteria count 100,000,000/gram), molasses fermented waste liquid 5 parts (being 60% containing organic mass percent), 37 parts of inorganic fertilizer (10 parts of carbamide, ammonium nitrate 8 parts, potassium dihydrogen phosphate 12 parts, potassium sulfate 7 parts), medium trace element 7.5 parts (Borax 3 parts, 2 parts of ferrous sulfate, 2.5 parts of EDTA-zinc), sodium alginate 0.2 part, the basic element of cell division 0.02 part, pH adjusting agent 1.25 parts, 23 parts of water.
Step:
(1) inorganic fertilizer is mixed, add water and stir and grind, form solution;
(2) in mixed solution, Borax, ferrous sulfate, EDTA-zinc, sodium alginate it are sequentially added into;
(3) heating is to 75 DEG C, adds molasses fermented waste liquid, reacts 1.5 hours;
(4) reactant liquor is down to room temperature;
(5) adding pH adjusting agent, regulating reactant liquor pH is 6, is slowly added to microbial inoculum, the basic element of cell division, obtains liquid microbial organic fertilizer A1.
Comparative example 2
Medium trace element in comparative example 2 increases to 20 parts, according to the mass fraction, proportioning raw materials: microbial inoculum 10 parts (bacillus subtilis 3 parts, bacillus megaterium 2 parts, bacillusmusilaginosiengineering 2 parts, colloid bacillus cereus 3 parts, living bacteria count 100,000,000/gram), molasses fermented waste liquid 25 parts (being 60% containing organic mass percent), 37 parts of inorganic fertilizer (10 parts of carbamide, ammonium nitrate 8 parts, potassium dihydrogen phosphate 12 parts, potassium sulfate 7 parts), medium trace element 20 parts (Borax 8 parts, 7 parts of ferrous sulfate, 5 parts of EDTA-zinc), sodium alginate 0.2 part, the basic element of cell division 0.02 part, pH adjusting agent 1.25 parts, 23 parts of water.
Step:
(1) inorganic fertilizer is mixed, add water and stir and grind, form solution;
(2) in mixed solution, Borax, ferrous sulfate, EDTA-zinc, sodium alginate it are sequentially added into;
(3) heating is to 75 DEG C, adds molasses fermented waste liquid, reacts 1.5 hours;
(4) reactant liquor is down to room temperature;
(5) adding pH adjusting agent, regulating reactant liquor pH is 6, is slowly added to microbial inoculum, the basic element of cell division, obtains liquid microbial organic fertilizer A2.
Comparative example 3
The pH value of reactant liquor is regulated to 9, in alkalescence. according to the mass fraction, proportioning raw materials: microbial inoculum 10 parts (bacillus subtilis 3 parts, bacillus megaterium 2 parts, bacillusmusilaginosiengineering 2 parts, colloid bacillus cereus 3 parts, living bacteria count 100,000,000/gram), molasses fermented waste liquid 25 parts (being 60% containing organic mass percent), 37 parts of inorganic fertilizer (10 parts of carbamide, ammonium nitrate 8 parts, potassium dihydrogen phosphate 12 parts, potassium sulfate 7 parts), medium trace element 7.5 parts (Borax 3 parts, 2 parts of ferrous sulfate, 2.5 parts of EDTA-zinc), sodium alginate 0.2 part, the basic element of cell division 0.02 part, pH adjusting agent 3 parts, 23 parts of water.
Step:
(1) inorganic fertilizer is mixed, add water and stir and grind, form solution;
(2) in mixed solution, Borax, ferrous sulfate, EDTA-zinc, sodium alginate it are sequentially added into;
(3) heating is to 75 DEG C, adds molasses fermented waste liquid, reacts 1.5 hours;
(4) reactant liquor is down to room temperature;
(5) adding pH adjusting agent, regulating reactant liquor pH is 9, is slowly added to microbial inoculum, the basic element of cell division, obtains liquid microbial organic fertilizer A3.
Comparative example 4
Compared with comparative example 1, it is adopt to add molasses fermented waste liquid under room temperature in step (3). according to the mass fraction, proportioning raw materials: microbial inoculum 10 parts (bacillus subtilis 3 parts, bacillus megaterium 2 parts, bacillusmusilaginosiengineering 2 parts, colloid bacillus cereus 3 parts, living bacteria count 100,000,000/gram), molasses fermented waste liquid 25 parts (being 60% containing organic mass percent), 37 parts of inorganic fertilizer (10 parts of carbamide, ammonium nitrate 8 parts, potassium dihydrogen phosphate 12 parts, potassium sulfate 7 parts), medium trace element 7.5 parts (Borax 3 parts, 2 parts of ferrous sulfate, 2.5 parts of EDTA-zinc), sodium alginate 0.2 part, the basic element of cell division 0.02 part, pH adjusting agent 1.25 parts, 23 parts of water.
Step:
(1) inorganic fertilizer is mixed, add water and stir and grind, form solution;
(2) in mixed solution, Borax, ferrous sulfate, EDTA-zinc, sodium alginate it are sequentially added into;
(3) add molasses fermented waste liquid under room temperature, react 1.5 hours;
(4) reactant liquor is down to room temperature;
(5) adding pH adjusting agent, regulating reactant liquor pH is 6, is slowly added to microbial inoculum, the basic element of cell division, obtains liquid microbial organic fertilizer A4.
The liquid microbial organic fertilizer that above-described embodiment 1 and comparative example 1-4 obtain has been made living bacteria count, the microorganism shelf-life, and the detection of the utilization rate of medium trace element, the detection method reference NYT2321-2013 to the viable count in microbial fermentation solution, table specific as follows:
Table 1
It is appreciated that from the data result of table 1, embodiment 1 and comparative example 1 are contrasted, living bacteria count in the microorganism fertilizer A prepared than embodiment 1 by the living bacteria count in the microorganism fertilizer A1 that the method for comparative example 1 prepares is few, this point can illustrate, the molasses fermented waste liquid adopting proportioning number of the present invention provides existence nutrient for microorganism fungus kind, it is more conducive to strain survival and reproduction, and the living bacteria count in microorganism fertilizer A is still a lot of after 12 months, in the present invention, the shelf-life of microorganism fertilizer is longer;
Embodiment 1 and comparative example 2 contrast, microorganism fertilizer A and microorganism fertilizer A2 is applied on soybean crops, under equal application conditions, preparation process with the addition of the microorganism fertilizer A2 of excessive medium trace element, medium trace element utilization rate have dropped on the contrary after application, this illustrate excessive medium trace element can not fully with the molasses fermented waste liquid chelating of proportioning of the present invention, the chelation percent of medium trace element declines;
Embodiment 1 and comparative example 3 contrast, and living bacteria count is decreased obviously, and regulate the pH value of microorganism fertilizer to 9, under this alkaline environment, are unfavorable for that strain is survived;
Embodiment 1 and comparative example 4 contrast, microorganism fertilizer A and microorganism fertilizer A4 is applied on soybean crops, under equal application conditions, add molasses fermented waste liquid at normal temperatures, the utilization rate of medium trace element is decreased obviously, and this illustrates that the temperature selected under the present invention is more conducive to molasses fermented waste liquid and medium trace element chelating, and chelate is more conducive to soybean crops and absorbs.
Embodiment 2
According to the mass fraction, proportioning raw materials: microbial inoculum 5 parts (bacillusmusilaginosiengineering 2 parts, colloid bacillus cereus 3 parts, living bacteria count 100,000,000/gram), fermented waste fluid 15 parts, inorganic fertilizer (5 parts of carbamide, ammonium nitrate 5 parts, potassium dihydrogen phosphate 10 parts, potassium sulfate 5 parts), medium trace element 4 (Borax 2 parts, 1 part of ferrous sulfate, 1 part of EDTA-zinc), 0.4 part of kieselguhr, the basic element of cell division 0.01 part, pH adjusting agent 0.5 part, 10 parts of water.
Step:
(1) inorganic fertilizer is mixed, add water and stir and grind, form solution;
(2) in mixed solution, Borax, trace element salt, kieselguhr it are sequentially added into;
(3) heating is to 50 DEG C, adds fermented waste fluid, reacts 0.5 hour;
(4) reactant liquor is down to room temperature;
(5) adding pH adjusting agent, regulating reactant liquor pH is 5.5, is slowly added to microbial inoculum, the basic element of cell division, obtains liquid microbial organic fertilizer.
Embodiment 3
According to the mass fraction, proportioning raw materials: microbial inoculum 15 parts (bacillus megaterium 5 parts, bacillusmusilaginosiengineering 5 parts, colloid bacillus cereus 5 parts, living bacteria count 100,000,000/gram), fermented waste fluid 30 parts, inorganic fertilizer (15 parts of carbamide, ammonium nitrate 10 parts, potassium dihydrogen phosphate 15 parts, potassium sulfate 10 parts), medium trace element 11 (Borax 4 parts, 3 parts of ferrous sulfate, 4 parts of EDTA-zinc), meerschaum 0.4 part, the basic element of cell division 0.03 part, pH adjusting agent 2 parts, 15 parts of water.
Step:
(1) inorganic fertilizer is mixed, add water and stir and grind, form solution;
(2) in mixed solution, Borax, trace element salt, meerschaum it are sequentially added into;
(3) heating is to 100 DEG C, adds fermented waste fluid, reacts 2 hours;
(4) reactant liquor is down to room temperature;
(5) adding pH adjusting agent, regulating reactant liquor pH is 6.5, is slowly added to microbial inoculum, the basic element of cell division, obtains liquid microbial organic fertilizer.
The announcement of book and instruction according to the above description, above-mentioned embodiment can also be modified and revise by those skilled in the art in the invention. Therefore, the invention is not limited in detailed description of the invention disclosed and described above, some modifications and changes of invention be should also be as falling in the scope of the claims of the present invention. Although additionally, employ some specific terms in this specification, but these terms are intended merely to convenient explanation, and the present invention does not constitute any restriction.
Claims (9)
1. a liquid microbial organic fertilizer, it is characterized in that, including containing the mixture of chelate, microbial inoculum, pH adjusting agent, count in mass ratio, mixture containing chelate: microbial inoculum: pH adjusting agent=19��41:5��15:0.5��2, the pH value of described liquid microbial organic fertilizer is 5.5��6.5, and the described mixture containing chelate includes medium trace element salt and fermented waste fluid, count in mass ratio, medium trace element salt: fermented waste fluid=4��11:15��30.
2. Liquid Fertilizer according to claim 1, it is characterized in that, the described mixture containing chelate also includes inorganic fertilizer, suspending agent, counts in mass ratio, medium trace element salt: fermented waste fluid: inorganic fertilizer: suspending agent=4��11:15��30:25��50:0.1��0.5.
3. Liquid Fertilizer according to claim 2, it is characterised in that the preparation method of the described mixture containing chelate is as follows:
(1) inorganic fertilizer is mixed, add water and stir and grind, form mixed solution;
(2) in mixed solution, medium trace element salt and suspending agent are added;
(3) heating is to 50��100 DEG C, adds fermented waste fluid, reacts 0.5��2 hour.
4. the Liquid Fertilizer according to Claims 2 or 3, it is characterised in that described inorganic fertilizer is two or more in carbamide, ammonium nitrate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, potassium sulfate, ammonium sulfate.
5. Liquid Fertilizer according to claim 1, it is characterised in that possibly together with the basic element of cell division in described liquid microbial organic fertilizer, by the mass ratio of strain, the addition of the described basic element of cell division is, microbial inoculum: the basic element of cell division=5��15:0.01��0.03.
6. Liquid Fertilizer according to claim 1, it is characterized in that, strain in described microbial inoculum is one or more in bacillus subtilis, bacillus megaterium, bacillusmusilaginosiengineering, colloid bacillus cereus, described microbial inoculum viable count is 20,000,000��200,000,000/gram.
7. Liquid Fertilizer according to claim 1, it is characterised in that described pH adjusting agent is one or more in potassium hydroxide, sodium hydroxide, concentrated sulphuric acid, concentrated nitric acid.
8. Liquid Fertilizer according to claim 1, it is characterised in that described medium trace element salt includes one or more in Borax, EDTA-zinc, zinc sulfate, ferrous sulfate heptahydrate, manganese sulfate, calcium salt, mantoquita.
9. Liquid Fertilizer according to claim 1, it is characterised in that described fermented waste fluid be molasses alcohol fermentation waste liquid and or amino acid fermentation waste liquid, the quality of organic matter content in described fermented waste fluid is 25%��60%.
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| CN106278628A (en) * | 2016-08-17 | 2017-01-04 | 常熟理工学院 | A kind of improve the method for strain survival rate in micro organism composite fertilizer |
| CN106699450A (en) * | 2016-12-15 | 2017-05-24 | 深圳市芭田生态工程股份有限公司 | Tuble vegetable function fertilizer |
| CN108178708A (en) * | 2018-01-25 | 2018-06-19 | 深圳市芭田生态工程股份有限公司 | A kind of selenium-rich organic carbon fertilizer and preparation method thereof |
| CN108383596A (en) * | 2018-04-03 | 2018-08-10 | 山东黎昊源生物工程有限公司 | A kind of liquid microbe fertilizers and preparation method thereof instead of chemical fertilizer |
| CN109180359A (en) * | 2018-11-18 | 2019-01-11 | 湖南海化生物肥业有限公司 | A kind of bio-feritlizer spray |
| CN109593013A (en) * | 2019-02-21 | 2019-04-09 | 中国科学院青海盐湖研究所 | Composite liquid fertilizer and preparation method thereof |
| CN112299924A (en) * | 2020-10-30 | 2021-02-02 | 广西百龙腾生物科技有限责任公司 | Novel efficient biochemical potassium fulvate and preparation method thereof |
| CN113135792A (en) * | 2021-04-28 | 2021-07-20 | 武汉富田生物科技有限公司 | Novel microbial liquid fertilizer |
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| CN106699450A (en) * | 2016-12-15 | 2017-05-24 | 深圳市芭田生态工程股份有限公司 | Tuble vegetable function fertilizer |
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| CN108383596A (en) * | 2018-04-03 | 2018-08-10 | 山东黎昊源生物工程有限公司 | A kind of liquid microbe fertilizers and preparation method thereof instead of chemical fertilizer |
| CN109180359A (en) * | 2018-11-18 | 2019-01-11 | 湖南海化生物肥业有限公司 | A kind of bio-feritlizer spray |
| CN109593013A (en) * | 2019-02-21 | 2019-04-09 | 中国科学院青海盐湖研究所 | Composite liquid fertilizer and preparation method thereof |
| CN112299924A (en) * | 2020-10-30 | 2021-02-02 | 广西百龙腾生物科技有限责任公司 | Novel efficient biochemical potassium fulvate and preparation method thereof |
| CN113135792A (en) * | 2021-04-28 | 2021-07-20 | 武汉富田生物科技有限公司 | Novel microbial liquid fertilizer |
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