CN102173399B - Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile - Google Patents
Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile Download PDFInfo
- Publication number
- CN102173399B CN102173399B CN201110054814XA CN201110054814A CN102173399B CN 102173399 B CN102173399 B CN 102173399B CN 201110054814X A CN201110054814X A CN 201110054814XA CN 201110054814 A CN201110054814 A CN 201110054814A CN 102173399 B CN102173399 B CN 102173399B
- Authority
- CN
- China
- Prior art keywords
- bacterium liquid
- thermoacidophile
- substratum
- low grade
- phosphate rock
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile. The method is characterized by comprising the following steps: 1) activating the original thermoacidophile liquid to obtain activated thermoacidophile liquid; 2) domesticating the obtain activated thermoacidophile liquid to obtain domesticated thermoacidophile liquid; and 3) putting a culture medium into a container and adding pyrite powder and mid-low-grade rock phosphate to obtain culture liquid C, adjusting the pH value of the culture liquid C to be 2.0-3.0 with sulfuric acid with concentration being 10-20wt% and then adding the domesticated thermoacidophile liquid; and then putting the container in a shaker at the constant temperature of 40-55 DEG C and carrying out shaking culture at a speed of 120-180r/min for 15-20 days to obtain soluble phosphorus. The method has the characteristics of high phosphorus solubility, simple process and low production cost.
Description
Technical field
The present invention relates to a kind of method of utilizing thermoacidophile dissolving mid low grade phosphate rock powder.
Background technology
In recent years, utilize the research of microbial technique processing Mineral resources very active, wherein the mikrobe of copper, uranium, gold is leached and has realized industrial application.Mikrobe is leached as a kind of environmental protection, and metallurgical method has efficiently become the main flow of modern metallurgical technology research gradually, has advantages such as low cost, simple to operate, environmental protection.
Phosphorus is very important nutritive element in plant-growth and the growth, is present in a large number in the phosphoric acid salt rock, and its main mineral forms is hydroxylapatite, fluorapatite and carbapatite etc., and they have constituted the staple of Rock Phosphate (72Min BPL).These ores are that the production of phosphate fertilizer provides cheap raw material, are the extraordinary substitutes of chemical phosphatic ferfilizer.According to statistics, about in the world Rock Phosphate (72Min BPL) of about 90% is used to produce various phosphate fertilizer, and working method mainly contains two kinds of wet method and Re Fa.Yet; No matter which kind of method of employing; Its raw material usually all need be than the phosphorus concentrate of higher-grade; And in the ore dressing and the course of processing, need huge facility investment and productive expense, this huge consumption and the environmental pollution that brings have thus caused great obstruction for the phosphate fertilizer industry in the whole world especially developing country.
China is the abundant country of a phosphate rock resource, but major part all is a mid low grade phosphate rock.These mid low grade phosphate rocks are to be in the status that goes out of use aspect the development and use of phosphate rock resource in China at present basically, and its reason just is that these mid low grade phosphate rock difficulties are adopted, difficult choosing, and foreign matter content is high.These factors have determined that Production Flow Chart is long when adopting conventional wet or thermal process phosphatic fertilizer production technique to handle these mid low grade phosphate rock resources, and production cost is high, and is low in economic efficiency.In view of this, the mikrobe leaching-out technique has received the many attention of People more and more gradually in the application aspect the phosphorus ore dissolving, understands to dissolve the phosphorus mikrobe and in the effect aspect the phosphorus ore dissolving development and utilization mid low grade phosphate rock resource is had crucial meaning.
Present research report has been found that multiple mikrobe such as bacterium, fungi and actinomycetes etc. all have the ability of dissolving insoluble inorganic phosphate compounds such as tricalcium phosphate, Lin Suanergai, hydroxylapatite and ground phosphate rock.But mentioned microorganism all belongs to organic chmosynthetic heterotrophs mikrobe (heterotrophic bacterium), and the mechanism of this quasi-microorganism decomposing phosphate rock is the solubilizing reaction that the microbiological deterioration organic carbon source produces some organic acid and phosphorus ore generation.In this process, phosphorus ore decomposes very slow, and pH value of solution only reaches 4.0～5.0, and molten phosphorus rate is lower.In addition, the culture condition of mikrobe is harsh, and substratum costs an arm and a leg and also limited the widespread usage of heterotrophic bacterium aspect the phosphorus ore dissolving.
Use for reference the mikrobe leaching-out technique, utilize the research of inorganic chemosynthetic autotroph mikrobe (autotrophic bacteria) dissolving phosphorus ore more and more to receive people's attention, like the Thiobacillus mikrobe etc.Yet; Utilize the research of autotrophic bacteria dissolving mid low grade phosphate rock also to be in the starting stage at present; The bacterial classification that uses also only limits to have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans), have a liking for acid oxidase sulphur thiobacillus (Acidithiobacillus thiooxidans) etc. have a liking in warm wtih; It is on the low side to dissolve the phosphorus rate, and the molten phosphorus cycle is longer.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing thermoacidophile dissolving mid low grade phosphate rock powder, this method has the advantages that molten phosphorus rate is high, technology is simple, production cost is low.
To achieve these goals, technical scheme of the present invention is: a kind of method of utilizing thermoacidophile dissolving mid low grade phosphate rock powder is characterized in that it comprises the steps:
1) activation of the original bacterium liquid of thermoacidophile:, obtain thermoacidophile activation bacterium liquid with the activation of the original bacterium liquid of thermoacidophile; The original bacterium liquid of described thermoacidophile is one of following 2 kinds: the original bacterium liquid of 1. thermophilic sulphur oxidation of sulfureted bacillus (Sulfobacillus thermosulfidooxidans); The mixed bacteria liquid of original bacterium liquid of 2. thermophilic sulphur oxidation of sulfureted bacillus and the original bacterium liquid of acidophilic thermophilic thiobacillus (Acidithiobacillus caldus), the volume proportion of original bacterium liquid of thermophilic sulphur oxidation of sulfureted bacillus and the original bacterium liquid of acidophilic thermophilic thiobacillus is 1 in the mixed bacteria liquid: (0.5～1.5);
2) domestication of thermoacidophile activation bacterium liquid:, obtain thermoacidophile domestication bacterium liquid with the domestication of thermoacidophile activation bacterium liquid;
3) thermoacidophile domestication bacterium liquid dissolving mid low grade phosphate rock powder: the add-on by thermoacidophile domestication bacterium liquid is 10～20% of a culture volume, chooses thermoacidophile domestication bacterium liquid and substratum; The proportioning of pressing pyritic ashes and substratum is (20～30) g: 1000mL, chooses pyritic ashes; The proportioning of pressing mid low grade phosphate rock powder and substratum is (10～20) g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, using concentration is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution C is 2.0～3.0, adds thermoacidophile again and tames bacterium liquid; Then container is placed 40～55 ℃ of constant temperature shaking tables, shaking culture is 15～20 days under 120～180 rev/mins of conditions, obtains titanium pigment.
According to technique scheme, the activation of the original bacterium liquid of described thermoacidophile is: the add-on by the original bacterium liquid of thermoacidophile is 10～20% of a culture volume, chooses original bacterium liquid of thermoacidophile and substratum; Substratum is packed in the container, and using concentration is that the pH that the sulfuric acid of 10～20wt% is regulated substratum is 2.0～3.0, adds the original bacterium liquid of thermoacidophile again; Then container is placed 40～55 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 100～180 rev/mins of conditions, obtains activation bacterium liquid A;
Add-on by activation bacterium liquid A is 10～20% of a culture volume again, chooses activation bacterium liquid A and substratum; Substratum is packed in the container, and using concentration is that the pH that the sulfuric acid of 10～20wt% is regulated substratum is 2.0～3.0, adds activation bacterium liquid A again; Then container is placed 40～55 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 100～180 rev/mins of conditions, obtains activatory activation bacterium liquid A once more; Inoculate activation so repeatedly 3～5 times, obtain thermoacidophile activation bacterium liquid.
According to technique scheme, being domesticated for of described thermoacidophile activation bacterium liquid: the add-on by thermoacidophile activation bacterium liquid is 20～30% of a culture volume, chooses thermoacidophile activation bacterium liquid and substratum; The proportioning of pressing pyritic ashes and substratum is (10～15) g: 1000mL, chooses pyritic ashes; The proportioning of pressing mid low grade phosphate rock powder and substratum is (5～10) g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, using concentration is that the pH of the sulfuric acid adjusting nutrient solution B of 10～20wt% is 2.0～3.0, adds thermoacidophile activation bacterium liquid again; Then container is placed 40～55 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 100～140 rev/mins of conditions, obtains taming bacterium liquid B;
Add-on by domestication bacterium liquid B is 20～30% of a culture volume again, chooses domestication bacterium liquid B and substratum; The proportioning of pressing pyritic ashes and substratum is (10～15) g: 1000mL, chooses pyritic ashes; The proportioning of pressing mid low grade phosphate rock powder and substratum is (5～10) g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, using concentration is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution B is 2.0～3.0, adds and tames bacterium liquid B; Then container is placed 40～55 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 100～140 rev/mins of conditions, the domestication bacterium liquid B that is tamed once more;
The inoculation domestication is 3～5 times so repeatedly; Each content of carrying pyritic ashes and mid low grade phosphate rock powder among the nutrient solution B gradually; In last prepared culture B: the proportioning of pyritic ashes and substratum=(20～30) g: 1000mL, the proportioning of mid low grade phosphate rock powder and substratum=(10～20) g: 1000mL; Obtain thermoacidophile domestication bacterium liquid.
Described thermophilic sulphur oxidation of sulfureted bacillus (Sulfobacillus thermosulfidooxidans), acidophilic thermophilic thiobacillus (Acidithiobacillus caldus) extensively are present in high-temperature acidic environment such as acid hot spring, acidic mine water, spontaneous combustion dump, sulfide ore stack or the high temperature bioleaching reactor drum, can obtain through buying from the nature separation screening or from DSMZ.
Described nutrient media components is: 3.0g (NH
4)
2SO
4, 1.0g MgSO
4.7H
2O, 0.2g K
2HPO
4, 0.2g KCl, 0.02gCa (NO
3)
2, 0.2g yeast extract, 30g FeSO
4.7H
2O and 1000mL sterilized water.
P in the described mid low grade phosphate rock powder
2O
5Mass content be lower than 30%.
Described pyritic ashes and mid low grade phosphate rock powder are the ore through market purchase or mine collection, and sieving through grinding makes its granularity reach 50～200 orders.
The invention has the beneficial effects as follows:
1, among the present invention with relative low price and the pyrite that obtains easily as the energy derive of thermoacidophile in process of growth, practiced thrift production cost, and positive effect also played in pyritous scientific and effective utilization.
2, the suitable temp of thermoacidophile growth is 40～55 ℃ among the present invention; Have than warm wtih in having a liking for as having a liking for the ferrous thiobacillus of acid oxidase, having a liking for stronger ferrous or sulphur oxidation capacities such as acid oxidase sulphur thiobacillus; Not only can improve the dissolved efficiency of mid low grade phosphate rock powder; Can also save simultaneously the refrigeration costs of system, increase economic efficiency.
3, this method can reach 60～80% to the molten phosphorus rate of mid low grade phosphate rock; Can utilize the mid low grade phosphate rock resource of China's abundant comparatively fully, for the scientific utilization resource, improve the level of resources utilization, keep China's agricultural sustainable development and phosphate fertilizer industrial environment and administer and have important practical significance.
4, technology of the present invention is simple, environmental friendliness.
Embodiment
In order to understand the present invention better, further illustrate content of the present invention below in conjunction with embodiment, but content of the present invention not only is confined to following embodiment.
Nutrient media components described in following examples is: 3.0g (NH
4)
2SO
4, 1.0g MgSO
4.7H
2O, 0.2g K
2HPO
4, 0.2gKCl, 0.02g Ca (NO
3)
2, 0.2g yeast extract, 30g FeSO
4.7H
2O and 1000mL sterilized water.
Embodiment 1:
A kind of method of utilizing thermoacidophile dissolving mid low grade phosphate rock powder, it comprises the steps:
1) activation of the original bacterium liquid of thermoacidophile: the add-on by the original bacterium liquid of thermoacidophile is 15% of a culture volume, chooses original bacterium liquid of thermoacidophile and substratum; Substratum is packed in the container (like triangular flask), and the pH that uses concentration to regulate substratum as the sulfuric acid of 10wt% is 2.5, adds the original bacterium liquid of thermoacidophile again; Then container is placed 45 ℃ of constant temperature shaking tables, shaking culture is 5 days under 140 rev/mins of conditions, obtains activation bacterium liquid A (the 1st activatory activation bacterium liquid A);
Add-on by activation bacterium liquid A is 15% of a culture volume again, chooses activation bacterium liquid A and substratum; Substratum is packed in the container, and the pH that uses concentration to regulate substratum as the sulfuric acid of 10wt% is 2.5, adds activation bacterium liquid A again; Then container is placed 45 ℃ of constant temperature shaking tables, shaking culture is 5 days under 140 rev/mins of conditions, obtains once more activatory activation bacterium liquid A (the 2nd activatory activation bacterium liquid A);
Be 15% of culture volume by the add-on of activatory activation bacterium liquid A once more again, choose activatory activation bacterium liquid A and substratum once more; Substratum is packed in the container, and the pH that uses concentration to regulate substratum as the sulfuric acid of 10wt% is 2.5, adds activatory activation bacterium liquid A once more again; Then container is placed 45 ℃ of constant temperature shaking tables, shaking culture is 5 days under 140 rev/mins of conditions, obtains activatory activation bacterium liquid A the 3rd time;
Add-on by the 3rd activatory activation bacterium liquid A is 15% of a culture volume again, chooses the 3rd activatory activation bacterium liquid A and substratum; Substratum is packed in the container, and the pH that uses concentration to regulate substratum as the sulfuric acid of 10wt% is 2.5, adds activatory activation bacterium liquid A again the 3rd time; Then container is placed 45 ℃ of constant temperature shaking tables, shaking culture is 5 days under 140 rev/mins of conditions, obtains thermoacidophile activation bacterium liquid (promptly inoculating activation 4 times so repeatedly);
The original bacterium liquid of described thermoacidophile is the original bacterium liquid of thermophilic sulphur oxidation of sulfureted bacillus (Sulfobacillus thermosulfidooxidans);
2) domestication of thermoacidophile activation bacterium liquid: the add-on by thermoacidophile activation bacterium liquid is 30% of a culture volume, chooses thermoacidophile activation bacterium liquid and substratum; Proportioning by pyritic ashes (200 order) and substratum is 15g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (50 order) and substratum is 5g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container (like triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, using concentration is 2.5 as the pH of the sulfuric acid adjusting nutrient solution B of 10wt%, adds thermoacidophile activation bacterium liquid again; Then container is placed 45 ℃ of constant temperature shaking tables, shaking culture is 3 days under 100 rev/mins of conditions, obtains taming bacterium liquid B;
Add-on (being inoculum size) by domestication bacterium liquid B is 30% of culture volume again, chooses domestication bacterium liquid B and substratum; Proportioning by pyritic ashes (200 order) and substratum is 18g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (50 order) and substratum is 7g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, the pH that uses concentration to regulate nutrient solution B as the sulfuric acid of 10wt% is 2.5, and bacterium liquid B is tamed in adding again; Then container is placed 45 ℃ of constant temperature shaking tables, shaking culture is 3 days under 100 rev/mins of conditions, the domestication bacterium liquid B that is tamed once more;
5 times (domestication 2 times have been inoculated in the front in the inoculation domestication so repeatedly; The repeated inoculation domestication is 3 times again); Each content of carrying pyritic ashes and mid low grade phosphate rock powder among the nutrient solution B gradually; In last prepared culture B: the proportioning=30g of pyritic ashes and substratum: 1000mL, the proportioning=20g of mid low grade phosphate rock powder and substratum: 1000mL; Obtain thermoacidophile domestication bacterium liquid;
[annotate, in the 3rd the inoculation domestication: the proportioning by pyritic ashes and substratum is 22g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder and substratum is 10g: 1000mL, chooses the mid low grade phosphate rock powder; In the 4th inoculation domestication: the proportioning by pyritic ashes and substratum is 25g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder and substratum is 15g: 1000mL, chooses the mid low grade phosphate rock powder; The difference of raising amount is gradually at every turn had no particular limits];
3) thermoacidophile domestication bacterium liquid dissolving mid low grade phosphate rock powder: the add-on by thermoacidophile domestication bacterium liquid is 12% of a culture volume, chooses thermoacidophile domestication bacterium liquid and substratum; Proportioning by pyritic ashes (200 order) and substratum is 30g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (50 order) and substratum is 20g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, using concentration is 2.5 as the pH of the sulfuric acid adjusting nutrient solution C of 10wt%, adds thermoacidophile again and tames bacterium liquid; Then container is placed 45 ℃ of constant temperature shaking tables, shaking culture is 18 days under 160 rev/mins of conditions, obtains titanium pigment.
P in the described mid low grade phosphate rock powder of present embodiment
2O
5Mass content be 20.8%, dissolve the phosphorus rate and reach 78.9%.
Embodiment 2:
A kind of method of utilizing thermoacidophile dissolving mid low grade phosphate rock powder, it comprises the steps:
1) activation of the original bacterium liquid of thermoacidophile: the add-on by the original bacterium liquid of thermoacidophile is 20% of a culture volume, chooses original bacterium liquid of thermoacidophile and substratum; Substratum is packed in the container (like triangular flask), and the pH that uses concentration to regulate substratum as the sulfuric acid of 15wt% is 2.8, adds the original bacterium liquid of thermoacidophile again; Then container is placed 50 ℃ of constant temperature shaking tables, shaking culture is 4 days under 120 rev/mins of conditions, obtains activation bacterium liquid A;
Add-on by activation bacterium liquid A is 20% of a culture volume again, chooses activation bacterium liquid A and substratum; Substratum is packed in the container, and the pH that uses concentration to regulate substratum as the sulfuric acid of 15wt% is 2.8, adds activation bacterium liquid A again; Then container is placed 50 ℃ of constant temperature shaking tables, shaking culture is 4 days under 120 rev/mins of conditions, obtains activatory activation bacterium liquid A once more; Inoculate activation so repeatedly 5 times (activation has been inoculated 2 times in the front, and the repeated inoculation activation is 3 times again), obtain thermoacidophile activation bacterium liquid.
The original bacterium liquid of described thermoacidophile is the original bacterium liquid of thermophilic sulphur oxidation of sulfureted bacillus (Sulfobacillus thermosulfidooxidans);
2) domestication of thermoacidophile activation bacterium liquid: the add-on by thermoacidophile activation bacterium liquid is 25% of a culture volume, chooses thermoacidophile activation bacterium liquid and substratum; Proportioning by pyritic ashes (100 order) and substratum is 10g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (100 order) and substratum is 8g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, using concentration is 2.8 as the pH of the sulfuric acid adjusting nutrient solution B of 15wt%, adds thermoacidophile activation bacterium liquid again; Then container is placed 50 ℃ of constant temperature shaking tables, shaking culture is 4 days under 120 rev/mins of conditions, obtains taming bacterium liquid B;
Add-on (being inoculum size) by domestication bacterium liquid B is 25% of culture volume again, chooses domestication bacterium liquid B and substratum; Proportioning by pyritic ashes (100 order) and substratum is 13g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (100 order) and substratum is 10g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, the pH that uses concentration to regulate nutrient solution B as the sulfuric acid of 15wt% is 2.8, and bacterium liquid B is tamed in adding again; Then container is placed 50 ℃ of constant temperature shaking tables, shaking culture is 4 days under 120 rev/mins of conditions, the domestication bacterium liquid B that is tamed once more;
4 times (domestication 2 times have been inoculated in the front in the inoculation domestication so repeatedly; The repeated inoculation domestication is 2 times again); Each content of carrying pyritic ashes and mid low grade phosphate rock powder among the nutrient solution B gradually; In last prepared culture B: the proportioning=20g of pyritic ashes and substratum: 1000mL, the proportioning=15g of mid low grade phosphate rock powder and substratum: 1000mL; Obtain thermoacidophile domestication bacterium liquid;
[annotate, in the 3rd the inoculation domestication: the proportioning by pyritic ashes and substratum is 17g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder and substratum is 12g: 1000mL, chooses the mid low grade phosphate rock powder; The difference of raising amount is gradually at every turn had no particular limits];
3) thermoacidophile domestication bacterium liquid dissolving mid low grade phosphate rock powder: the add-on by thermoacidophile domestication bacterium liquid is 15% of a culture volume, chooses thermoacidophile domestication bacterium liquid and substratum; Proportioning by pyritic ashes (100 order) and substratum is 20g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (100 order) and substratum is 15g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, using concentration is 2.8 as the pH of the sulfuric acid adjusting nutrient solution C of 15wt%, adds thermoacidophile again and tames bacterium liquid; Then container is placed 50 ℃ of constant temperature shaking tables, shaking culture is 15 days under 180 rev/mins of conditions, obtains titanium pigment.
P in the described mid low grade phosphate rock powder of present embodiment
2O
5Mass content be 17.5%, dissolve the phosphorus rate and reach 65.7%.
Embodiment 3:
A kind of method of utilizing thermoacidophile dissolving mid low grade phosphate rock powder, it comprises the steps:
1) activation of the original bacterium liquid of thermoacidophile: the add-on by the original bacterium liquid of thermoacidophile is 12% of a culture volume, chooses original bacterium liquid of thermoacidophile and substratum; Substratum is packed in the container, and the pH that uses concentration to regulate substratum as the sulfuric acid of 20wt% is 2.2, adds the original bacterium liquid of thermoacidophile again; Then container is placed 42 ℃ of constant temperature shaking tables, shaking culture is 4 days under 130 rev/mins of conditions, obtains activation bacterium liquid A;
Add-on by activation bacterium liquid A is 12% of a culture volume again, chooses activation bacterium liquid A and substratum; Substratum is packed in the container, and the pH that uses concentration to regulate substratum as the sulfuric acid of 20wt% is 2.2, adds activation bacterium liquid A again; Then container is placed 42 ℃ of constant temperature shaking tables, shaking culture is 4 days under 130 rev/mins of conditions, obtains activatory activation bacterium liquid A once more; Inoculate activation so repeatedly 5 times (activation has been inoculated 2 times in the front, and the repeated inoculation activation is 3 times again), obtain thermoacidophile activation bacterium liquid.
The original bacterium liquid of described thermoacidophile is the mixed bacteria liquid of original bacterium liquid of thermophilic sulphur oxidation of sulfureted bacillus (Sulfobacillusthermosulfidooxidans) and the original bacterium liquid of acidophilic thermophilic thiobacillus (Acidi thiobacillus caldus), and the volume ratio of original bacterium liquid of thermophilic sulphur oxidation of sulfureted bacillus and the original bacterium liquid of acidophilic thermophilic thiobacillus is 1: 1 in the mixed bacteria liquid;
2) domestication of thermoacidophile activation bacterium liquid: the add-on by thermoacidophile activation bacterium liquid is 25% of a culture volume, chooses thermoacidophile activation bacterium liquid and substratum; Proportioning by pyritic ashes (100 order) and substratum is 12g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (50 order) and substratum is 10g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, using concentration is 2.2 as the pH of the sulfuric acid adjusting nutrient solution B of 20wt%, adds thermoacidophile activation bacterium liquid again; Then container is placed 42 ℃ of constant temperature shaking tables, shaking culture is 4 days under 140 rev/mins of conditions, obtains taming bacterium liquid B;
The 2nd inoculation domestication: the add-on (being inoculum size) by domestication bacterium liquid B is 25% of culture volume, chooses domestication bacterium liquid B and substratum; Proportioning by pyritic ashes (100 order) and substratum is 15g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (50 order) and substratum is 12g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, the pH that uses concentration to regulate nutrient solution B as the sulfuric acid of 20wt% is 2.2, and bacterium liquid B is tamed in adding again; Then container is placed 42 ℃ of constant temperature shaking tables, shaking culture is 4 days under 140 rev/mins of conditions, obtains the domestication bacterium liquid B of the 2nd domestication;
The 3rd inoculation domestication: the add-on (being inoculum size) by the domestication bacterium liquid B of the 2nd domestication is 25% of culture volume, chooses the domestication bacterium liquid B and the substratum of taming for the 2nd time; Proportioning by pyritic ashes (100 order) and substratum is 20g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (50 order) and substratum is 15g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, using concentration is 2.2 as the pH of the sulfuric acid adjusting nutrient solution B of 20wt%, adds the domestication bacterium liquid B that tames for the 2nd time again; Then container is placed 42 ℃ of constant temperature shaking tables, shaking culture is 4 days under 140 rev/mins of conditions, obtains thermoacidophile domestication bacterium liquid (promptly inoculation is tamed 3 times so repeatedly);
3) thermoacidophile domestication bacterium liquid dissolving mid low grade phosphate rock powder: the add-on by thermoacidophile domestication bacterium liquid is 18% of a culture volume, chooses thermoacidophile domestication bacterium liquid and substratum; Proportioning by pyritic ashes (100 order) and substratum is 20g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (50 order) and substratum is 15g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, using concentration is 2.2 as the pH of the sulfuric acid adjusting nutrient solution C of 20wt%, adds thermoacidophile again and tames bacterium liquid; Then container is placed 42 ℃ of constant temperature shaking tables, shaking culture is 20 days under 160 rev/mins of conditions, obtains titanium pigment.
P in the described mid low grade phosphate rock powder of present embodiment
2O
5Mass content be 21.6%, dissolve the phosphorus rate and reach 72.3%.
Embodiment 4:
A kind of method of utilizing thermoacidophile dissolving mid low grade phosphate rock powder, it comprises the steps:
1) activation of the original bacterium liquid of thermoacidophile: the add-on by the original bacterium liquid of thermoacidophile is 15% of a culture volume, chooses original bacterium liquid of thermoacidophile and substratum; Substratum is packed in the container, and the pH that uses concentration to regulate substratum as the sulfuric acid of 10wt% is 2.0, adds the original bacterium liquid of thermoacidophile again; Then container is placed 40 ℃ of constant temperature shaking tables, shaking culture is 5 days under 140 rev/mins of conditions, obtains activation bacterium liquid A;
Add-on by activation bacterium liquid A is 15% of a culture volume again, chooses activation bacterium liquid A and substratum; Substratum is packed in the container, and the pH that uses concentration to regulate substratum as the sulfuric acid of 10wt% is 2.0, adds activation bacterium liquid A again; Then container is placed 40 ℃ of constant temperature shaking tables, shaking culture is 5 days under 140 rev/mins of conditions, obtains activatory activation bacterium liquid A once more; Inoculate activation so repeatedly 4 times (activation has been inoculated 2 times in the front, and the repeated inoculation activation is 2 times again), obtain thermoacidophile activation bacterium liquid.
The original bacterium liquid of described thermoacidophile is the mixed bacteria liquid of original bacterium liquid of thermophilic sulphur oxidation of sulfureted bacillus (Sulfobacillusthermosulfidooxidans) and the original bacterium liquid of acidophilic thermophilic thiobacillus (Acidi thiobacillus caldus), and the volume proportion of original bacterium liquid of thermophilic sulphur oxidation of sulfureted bacillus and the original bacterium liquid of acidophilic thermophilic thiobacillus is 1: 1.2 in the mixed bacteria liquid;
2) domestication of thermoacidophile activation bacterium liquid: the add-on by thermoacidophile activation bacterium liquid is 30% of a culture volume, chooses thermoacidophile activation bacterium liquid and substratum; Proportioning by pyritic ashes (200 order) and substratum is 15g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (50 order) and substratum is 5g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, using concentration is 2.0 as the pH of the sulfuric acid adjusting nutrient solution B of 10wt%, adds thermoacidophile activation bacterium liquid again; Then container is placed 40 ℃ of constant temperature shaking tables, shaking culture is 3 days under 100 rev/mins of conditions, obtains taming bacterium liquid B;
Add-on (being inoculum size) by thermoacidophile domestication bacterium liquid B is 30% of culture volume again, chooses domestication bacterium liquid B and substratum; Proportioning by pyritic ashes (200 order) and substratum is 20g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (50 order) and substratum is 8g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, the pH that uses concentration to regulate nutrient solution B as the sulfuric acid of 10wt% is 2.0, and bacterium liquid B is tamed in adding again; Then container is placed 40 ℃ of constant temperature shaking tables, shaking culture is 3 days under 100 rev/mins of conditions, the domestication bacterium liquid B that is tamed once more;
4 times (domestication 2 times have been inoculated in the front in the inoculation domestication so repeatedly; The repeated inoculation domestication is 2 times again); Each content of carrying pyritic ashes and mid low grade phosphate rock powder among the nutrient solution B gradually; In last prepared culture B: the proportioning=28g of pyritic ashes and substratum: 1000mL, the proportioning=16g of mid low grade phosphate rock powder and substratum: 1000mL; Obtain thermoacidophile domestication bacterium liquid;
[annotate, in the 3rd the inoculation domestication: the proportioning by pyritic ashes and substratum is 25g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder and substratum is 12g: 1000mL, chooses the mid low grade phosphate rock powder; The difference of raising amount is gradually at every turn had no particular limits];
3) thermoacidophile domestication bacterium liquid dissolving mid low grade phosphate rock powder: the add-on by thermoacidophile domestication bacterium liquid is 10% of a culture volume, chooses thermoacidophile domestication bacterium liquid and substratum; Proportioning by pyritic ashes (200 order) and substratum is 30g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (50 order) and substratum is 16g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, using concentration is 2.0 as the pH of the sulfuric acid adjusting nutrient solution C of 10wt%, adds thermoacidophile again and tames bacterium liquid; Then container is placed 40 ℃ of constant temperature shaking tables, shaking culture is 15 days under 120 rev/mins of conditions, obtains titanium pigment.
P in the described mid low grade phosphate rock powder of present embodiment
2O
5Mass content be 23.1%, dissolve the phosphorus rate and reach 69.4%.
Embodiment 5:
A kind of method of utilizing thermoacidophile dissolving mid low grade phosphate rock powder, it comprises the steps:
1) activation of the original bacterium liquid of thermoacidophile: the add-on by the original bacterium liquid of thermoacidophile is 20% of a culture volume, chooses original bacterium liquid of thermoacidophile and substratum; Substratum is packed in the container, and the pH that uses concentration to regulate substratum as the sulfuric acid of 20wt% is 3.0, adds the original bacterium liquid of thermoacidophile again; Then container is placed 55 ℃ of constant temperature shaking tables, shaking culture is 5 days under 140 rev/mins of conditions, obtains activation bacterium liquid A;
Add-on by activation bacterium liquid A is 20% of a culture volume again, chooses activation bacterium liquid A and substratum; Substratum is packed in the container, and the pH that uses concentration to regulate substratum as the sulfuric acid of 20wt% is 3.0, adds activation bacterium liquid A again; Then container is placed 55 ℃ of constant temperature shaking tables, shaking culture is 5 days under 140 rev/mins of conditions, obtains activatory activation bacterium liquid A once more; Inoculate activation so repeatedly 3 times (activation has been inoculated 2 times in the front, and the repeated inoculation activation is 1 time again), obtain thermoacidophile activation bacterium liquid.
The original bacterium liquid of described thermoacidophile is the mixed bacteria liquid of original bacterium liquid of thermophilic sulphur oxidation of sulfureted bacillus (Sulfobacillusthermosulfidooxidans) and the original bacterium liquid of acidophilic thermophilic thiobacillus (Acidi thiobacillus caldus), and the volume proportion of original bacterium liquid of thermophilic sulphur oxidation of sulfureted bacillus and the original bacterium liquid of acidophilic thermophilic thiobacillus is 1: 0.5 in the mixed bacteria liquid;
2) domestication of thermoacidophile activation bacterium liquid: the add-on by thermoacidophile activation bacterium liquid is 25% of a culture volume, chooses thermoacidophile activation bacterium liquid and substratum; Proportioning by pyritic ashes (50 order) and substratum is 15g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (100 order) and substratum is 10g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, using concentration is 3.0 as the pH of the sulfuric acid adjusting nutrient solution B of 20wt%, adds thermoacidophile activation bacterium liquid again; Then container is placed 55 ℃ of constant temperature shaking tables, shaking culture is 3 days under 120 rev/mins of conditions, obtains taming bacterium liquid B;
The 2nd inoculation domestication: the add-on (being inoculum size) by domestication bacterium liquid B is 25% of culture volume, chooses domestication bacterium liquid B and substratum; Proportioning by pyritic ashes (50 order) and substratum is 22g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (100 order) and substratum is 12g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, the pH that uses concentration to regulate nutrient solution B as the sulfuric acid of 20wt% is 3.0, and bacterium liquid B is tamed in adding again; Then container is placed 55 ℃ of constant temperature shaking tables, shaking culture is 3 days under 120 rev/mins of conditions, the domestication bacterium liquid B that is tamed once more;
The 3rd inoculation domestication: the add-on (being inoculum size) by the domestication bacterium liquid B that tames once more is 25% of culture volume, chooses the domestication bacterium liquid B and the substratum of domestication once more; Proportioning by pyritic ashes (50 order) and substratum is 25g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (100 order) and substratum is 15g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, the pH that uses concentration to regulate nutrient solution B as the sulfuric acid of 20wt% is 3.0, again the domestication bacterium liquid B that tames once more of adding; Then container is placed 55 ℃ of constant temperature shaking tables, shaking culture is 3 days under 120 rev/mins of conditions, obtains thermoacidophile domestication bacterium liquid (promptly inoculation is tamed 3 times so repeatedly);
3) thermoacidophile domestication bacterium liquid dissolving mid low grade phosphate rock powder: the add-on by thermoacidophile domestication bacterium liquid is 10% of a culture volume, chooses thermoacidophile domestication bacterium liquid and substratum; Proportioning by pyritic ashes (50 order) and substratum is 25g: 1000mL, chooses pyritic ashes; Proportioning by mid low grade phosphate rock powder (100 order) and substratum is 10g: 1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, using concentration is 3.0 as the pH of the sulfuric acid adjusting nutrient solution C of 20wt%, adds thermoacidophile again and tames bacterium liquid; Then container is placed 55 ℃ of constant temperature shaking tables, shaking culture is 15 days under 160 rev/mins of conditions, obtains titanium pigment.
P in the described mid low grade phosphate rock powder of present embodiment
2O
5Mass content be 23.5%, dissolve the phosphorus rate and reach 70.4%.
The bound of each raw material that the present invention is cited, interval value, and the bound of processing parameter (like temperature, time etc.), interval value can both realize the present invention, do not enumerate embodiment one by one at this.
Claims (4)
1. a method of utilizing thermoacidophile dissolving mid low grade phosphate rock powder is characterized in that it comprises the steps:
1) activation of the original bacterium liquid of thermoacidophile:, obtain thermoacidophile activation bacterium liquid with the activation of the original bacterium liquid of thermoacidophile; The original bacterium liquid of described thermoacidophile is one of following 2 kinds: 1. thermophilic sulphur oxidation of sulfureted bacillus (
Sulfobacillus thermosulfidooxidans) original bacterium liquid, original bacterium liquid of 2. thermophilic sulphur oxidation of sulfureted bacillus and acidophilic thermophilic thiobacillus (
Acidithiobacillus caldus) mixed bacteria liquid of original bacterium liquid, the volume proportion of original bacterium liquid of thermophilic sulphur oxidation of sulfureted bacillus and the original bacterium liquid of acidophilic thermophilic thiobacillus is 1 in the mixed bacteria liquid: (0.5～1.5);
The activation of the original bacterium liquid of described thermoacidophile is: the add-on by the original bacterium liquid of thermoacidophile is 10～20% of a culture volume, chooses original bacterium liquid of thermoacidophile and substratum; Substratum is packed in the container, and using concentration is that the pH that the sulfuric acid of 10～20wt% is regulated substratum is 2.0～3.0, adds the original bacterium liquid of thermoacidophile again; Then container is placed 40～55 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 100～180 rev/mins of conditions, obtains activation bacterium liquid A;
Add-on by activation bacterium liquid A is 10～20% of a culture volume again, chooses activation bacterium liquid A and substratum; Substratum is packed in the container, and using concentration is that the pH that the sulfuric acid of 10～20wt% is regulated substratum is 2.0～3.0, adds activation bacterium liquid A again; Then container is placed 40～55 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 100～180 rev/mins of conditions, obtains activatory activation bacterium liquid A once more; Inoculate activation so repeatedly 3～5 times, obtain thermoacidophile activation bacterium liquid;
2) domestication of thermoacidophile activation bacterium liquid:, obtain thermoacidophile domestication bacterium liquid with the domestication of thermoacidophile activation bacterium liquid;
Being domesticated for of described thermoacidophile activation bacterium liquid: the add-on by thermoacidophile activation bacterium liquid is 20～30% of a culture volume, chooses thermoacidophile activation bacterium liquid and substratum; The proportioning of pressing pyritic ashes and substratum is (10～15) g:1000mL, chooses pyritic ashes; The proportioning of pressing mid low grade phosphate rock powder and substratum is (5～10) g:1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution B, using concentration is that the pH of the sulfuric acid adjusting nutrient solution B of 10～20wt% is 2.0～3.0, adds thermoacidophile activation bacterium liquid again; Then container is placed 40～55 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 100～140 rev/mins of conditions, obtains taming bacterium liquid B;
The inoculation domestication is 3～5 times so repeatedly; Each content that improves pyritic ashes and mid low grade phosphate rock powder among the nutrient solution B gradually; In last prepared culture B: the proportioning of pyritic ashes and substratum=(20～30) g:1000mL, the proportioning of mid low grade phosphate rock powder and substratum=(10～20) g:1000mL; Obtain thermoacidophile domestication bacterium liquid;
3) thermoacidophile domestication bacterium liquid dissolving mid low grade phosphate rock powder: the add-on by thermoacidophile domestication bacterium liquid is 10～20% of a culture volume, chooses thermoacidophile domestication bacterium liquid and substratum; The proportioning of pressing pyritic ashes and substratum is (20～30) g:1000mL, chooses pyritic ashes; The proportioning of pressing mid low grade phosphate rock powder and substratum is (10～20) g:1000mL, chooses the mid low grade phosphate rock powder; Substratum is packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, using concentration is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution C is 2.0～3.0, adds thermoacidophile again and tames bacterium liquid; Then container is placed 40～55 ℃ of constant temperature shaking tables, shaking culture is 15～20 days under 120～180 rev/mins of conditions, obtains titanium pigment.
2. a kind of method of utilizing thermoacidophile dissolving mid low grade phosphate rock powder according to claim 1, it is characterized in that: described nutrient media components is: 3.0g (NH
4)
2SO
4, 1.0g MgSO
47H
2O, 0.2g K
2HPO
4, 0.2g KCl, 0.02g Ca (NO
3)
2, 0.2g yeast extract, 30g FeSO
47H
2O and 1000mL sterilized water.
3. a kind of method of utilizing thermoacidophile dissolving mid low grade phosphate rock powder according to claim 1 is characterized in that: the P in the described mid low grade phosphate rock powder
2O
5Mass content be lower than 30%, the particle diameter of mid low grade phosphate rock powder is 50～200 orders.
4. a kind of method of utilizing thermoacidophile dissolving mid low grade phosphate rock powder according to claim 1, it is characterized in that: the particle diameter of pyritic ashes is 50～200 orders.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110054814XA CN102173399B (en) | 2011-03-08 | 2011-03-08 | Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110054814XA CN102173399B (en) | 2011-03-08 | 2011-03-08 | Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102173399A CN102173399A (en) | 2011-09-07 |
| CN102173399B true CN102173399B (en) | 2012-11-21 |
Family
ID=44516707
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201110054814XA Expired - Fee Related CN102173399B (en) | 2011-03-08 | 2011-03-08 | Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102173399B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CL2011003066A1 (en) * | 2011-12-02 | 2012-06-29 | Biosigma Sa | Method for detecting the sulfo-oxidant activity of a microbiological process, based on detecting the presence in the extracellular environment of the biomarker spermidine produced by the sulfo-oxidant microorganisms participating in said process. |
| CN106148244B (en) * | 2016-08-30 | 2019-04-09 | 重庆工商大学 | It is a kind of degrade waste lubricating oil Acidovorax avenae and purposes |
| CN108753615A (en) * | 2018-05-24 | 2018-11-06 | 江口县旭辉生态农业科技有限公司 | A method of dissolving low grade rock phosphate using Haloragidaceae Myriophyllum spicatum endophyte |
| CN109576173B (en) * | 2018-12-04 | 2020-05-08 | 江南大学 | Acidithiobacillus caldus and application thereof |
| CN111069237B (en) * | 2019-12-20 | 2021-07-13 | 武汉工程大学 | Medium-low grade phosphorite heap leaching method combining composite strain with waste biomass |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1827805A (en) * | 2006-03-23 | 2006-09-06 | 福州大学 | Sulfide ore treatment technology by thermoacidophile |
| CN101191152A (en) * | 2006-11-28 | 2008-06-04 | 北京有色金属研究总院 | Biological stirring cobalt-dipping technique for concentrate containing cobalt and sulfur |
| CN101434917A (en) * | 2007-11-13 | 2009-05-20 | 北京有色金属研究总院 | Acidophilic leaching-ore bacteria, heap bioleaching process for low grade phosphate ore by using the same |
| CN101837962A (en) * | 2009-12-01 | 2010-09-22 | 西部矿业股份有限公司 | Method for leaching phosphorus from low-grade phosphate ore by thiobacillus thiooxidans |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AUPQ265199A0 (en) * | 1999-09-03 | 1999-09-30 | Pacific Ore Technology Limited | Improved bacterial oxidation of sulphide ores and concentrates |
-
2011
- 2011-03-08 CN CN201110054814XA patent/CN102173399B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1827805A (en) * | 2006-03-23 | 2006-09-06 | 福州大学 | Sulfide ore treatment technology by thermoacidophile |
| CN101191152A (en) * | 2006-11-28 | 2008-06-04 | 北京有色金属研究总院 | Biological stirring cobalt-dipping technique for concentrate containing cobalt and sulfur |
| CN101434917A (en) * | 2007-11-13 | 2009-05-20 | 北京有色金属研究总院 | Acidophilic leaching-ore bacteria, heap bioleaching process for low grade phosphate ore by using the same |
| CN101837962A (en) * | 2009-12-01 | 2010-09-22 | 西部矿业股份有限公司 | Method for leaching phosphorus from low-grade phosphate ore by thiobacillus thiooxidans |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102173399A (en) | 2011-09-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102329957B (en) | Method for continuously leaching sulfide ore by using synergy of autotrophic ore leaching bacteria and heterotrophic ore leaching bacteria | |
| CN102173399B (en) | Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile | |
| CN106008101A (en) | Composite microbial organic fertilizer for saline-alkali soil improvement and preparation method thereof | |
| CN104788265A (en) | Saline-alkali soil biological improved fertilizer based on high-sulfur gangue and preparation method thereof | |
| Li et al. | Evaluation and improvement of phosphate solubilization by an isolated bacterium Pantoea agglomerans ZB | |
| CN105646085A (en) | Liquid organic microbial fertilizer | |
| CN101628830B (en) | Method for reducing nitrogen loss during composting process | |
| CN101328089B (en) | Method for producing composite microorganism phosphate using low grade ground phosphate rock | |
| CN105016849A (en) | Sulphur-based gamma-biological compound fertilizer and preparation method thereof | |
| CN103014336B (en) | Preparation and method for treating high-arsenic and high-sulfur gold ore | |
| CN103013847A (en) | Ammonia-producing mineral leaching bacterium JAT-1 as well as culture method and application of ammonia-producing mineral leaching bacterium JAT-1 | |
| CN114375797A (en) | Ecological remediation soil matrix and preparation method and application thereof | |
| CN103031434A (en) | Refractory gold ore desulfurizing and dearsenifying method | |
| Xiao et al. | Characterization of tricalcium phosphate solubilization by Stenotrophomonas maltophilia YC isolated from phosphate mines | |
| CN102432363B (en) | Method for preparing bacterial composite phosphate fertilizer with medium and low grade phosphate ores | |
| CN100345962C (en) | Method for transferring phosphor of phosphorite to soluble phosphor using thiobacillus microorganism | |
| CN102173878A (en) | Method for dissolving medium/low grade phosphate ore powder by utilizing acidophilic heterotroph and acidophilic autotroph | |
| CN102174585B (en) | Method for dissolving mid-low-grade rock phosphate by utilizing mixed bacterial liquid | |
| CN103088222B (en) | Process for treating refractory gold ores | |
| CN105859081B (en) | A kind of nutrient special and its production method for sludge organism processing | |
| CN101356890A (en) | Tobacco floating-seedling substrate, preparation method and use thereof | |
| CN116042408A (en) | Phosphate-solubilizing bacterium and application thereof | |
| CN101597037B (en) | Method for bioleaching phosphor in iron ore containing phosphor | |
| CN103205571B (en) | Treatment process for high-carbon high-arsenic gold ores | |
| CN103205381B (en) | Decarbonizing and desulphurizing bacterium agent for difficultly-selected gold ore and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20121121 Termination date: 20160308 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |