CN101837962A - Method for leaching phosphorus from low-grade phosphate ore by thiobacillus thiooxidans - Google Patents
Method for leaching phosphorus from low-grade phosphate ore by thiobacillus thiooxidans Download PDFInfo
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Abstract
The invention relates to microbial leaching technology, in particular to a microbial leaching method for low-grade phosphate ore by thiobacillus thiooxidans separated from a copper ore soil sample in Tongshankou in Hubei Province. The method comprises the following steps of: 1) sampling; 2) enrichment, separation and domestication of bacterial strain; and 3) leaching method for phosphorus. The bacterial strain which is obtained by screening and domesticating makes the phosphorus leaching rate over 30 percent higher than that in the conventional reports. By using pyrite as a leaching aid, the leaching method reduces the addition of a great amount of expensive phosphorous compounds and monomer sulfur, is favorable for cycle and high-efficiency utilization of accompanying resources in the phosphorite, reduces the environmental pollution and the investment cost, has lower production cost, and can realize sustainable development of the resource development and environmental protection.
Description
Technical field
The present invention relates to a kind of microbial leaching technology, specifically relate to obtain a strain thiobacillus thiooxidans a kind of from the brass hill mouth copper mine soil sample of Hubei, the separation, be applied to the microbe leaching-out method of the low-grade phosphate ore of low-grade phosphate ore leaching.
Background technology
China's phosphate rock resource is abundant, and about 50,000,000,000 tons of total resources is distributed in 26 provinces, concentrates on relatively within cloud, expensive, river, Hubei Province, 5 provinces in Hunan, accounts for 4/5 of national total reserves.The characteristics of the phosphate rock resource of China: (1) aboundresources, but it is too concentrated to distribute; (2) mid low grade phosphate rock is many, rich ore is few; (3) difficult ore dressing is many, and easily ore dressing is few; Be suitable for lacking of extensive high strength exploitation.Along with the development of process of industrialization, people constantly enlarge the demand of raw material, and the higher-grade rich ore has been developed totally, and we are obliged to go to utilize a large amount of low-grade, unmanageable lean ore resources.
Traditional dressing and smelting process shows as that efficient is low, long flow path, production cost height, environmental pollution be serious when handling the low-grade complex Mineral resources.And the technology of a kind of just low cost of biological metallurgy technology (claiming the bioleaching technology), environmental friendliness, highly-efficient processing Mineral resources.It is oxidation, reduction, dissolving, the absorption effects such as (absorption) that utilizes certain micro-organisms or its meta-bolites that some mineral (being mainly sulfide mineral) and element are had, and moltenly from ore soaks metal or reclaim the hydrometallurgy process that (removing) has valency (being harmful to) metal from water.
The history of the existing many decades of the development of biological metallurgy technology, at present microbial leaching economically valuable be copper, uranium and gold.Particularly extract copper with the bacteria heap leaching method, be microorganism in a most successful direction of mining and metallurgy industrial application, countries such as the U.S., Chile, Australia, Canada all once carried out the production that bacteria heap leaching reclaims copper mine stone in low grade ore or the difficult stone of digging up mine.The U.S. and Chile utilize biological hydrometallurgy to carry copper and account for more than 11% and 20% of the copper total amount of producing of this country respectively.At home, the research of microbial leaching originates in nineteen sixties the earliest, and institute of microbiology of the Chinese Academy of Sciences has carried out experimental study to copper official mountain copper mine.80 to the nineties, and Institute of Micro-biology of the Chinese Academy of Sciences, chemical industry metallurgical institute of the Chinese Academy of Sciences etc. has carried out extensive studies to the biological extraction of low grade ores such as copper, nickel and the theory and the technology of high-Arsen gold ore preoxidation respectively.The nineties middle and later periods, low-grade copper mine biological extraction technology is in Jiangxi Copper company Dexing copper mine successful Application, and builds up the dump leaching factory that produces 2000 tons of electrolytic coppers per year, is the factory that first biology that China builds up is carried copper.
The development and use research work of the low-grade phosphate ore of China at present relatively lags behind, along with the dilution of phosphate rock resource with to high-purity and high added value phosphorus chemical product increase in demand, problem how to utilize low-grade phosphate ore to produce high-quality product also just displays, and utilizes the method for microbial leaching to handle can yet be regarded as the choosing of going up in the method for low-grade phosphate ore.
Microorganism about phosphorus ore is leached, and some reports have been arranged both at home and abroad.According to physio-biochemical characteristics, the microorganism that can be used for the phosphorus ore leaching can be divided into chemoheterotrophy microorganism and chemoautotrophy microorganism.About the molten phosphorus of heterotrophic bacterium, carried out a large amount of research both at home and abroad.The result who has obtained shows that the organic acid acidity that the heterotrophic bacterium metabolism produces is not strong, and the speed of decomposing phosphate rock is slow, and leaching yield is low, and the experiment suitability for industrialized production is very difficult, is mainly used in the phosphate fertilizer of biologically active.And about the molten phosphorus of autotrophic bacteria, report both domestic and external is less, mainly is to utilize the iron of thiobacillus ferrooxidans's redox state or sulphur to produce sulfuric acid the dissolving phosphorus ore.Because sulfuric acid has stronger molten phosphorus effect, and the cultivation of autotrophic bacteria do not need organic nutrient substance, and is energy autophyting growth with reduced sulfur or iron mineral association in the phosphorus ore or that add, therefore has the possibility of suitability for industrialized production.
Thiobacillus has certain achievement to the leaching of phosphorus ore, and according to nearest achievements in research such as Gong Wenqi, the phosphorus rate of soaking of thiobacillus thiooxidans can reach 57.7% on the basis of having added Tween60, and general bacterium is soaked the phosphorus rate and all remains on 40%-60%.But this technology extraction time is long, and for 15-20d soaks the ore deposit, people such as Wang Guanghua study 3 kinds of molten phosphorus fungi dissolved phosphorus breeze abilities different nitrogen sources, and its test strain is a fungi, and lacks the test to processing parameter.
In a word, it is few to utilize thiobacillus thiooxidans to carry out the research that phosphorus ore leaches, and its maximum shortcoming is that the production cycle is long, and ore-leaching speed is slow, and dump leaching, original place leaching generally need some months even several years.Improve speed and leaching yield particularly important that bacterium is leached mineral, and the seed selection strain excellent is to improve the key of soaking the ore deposit rate, simultaneously, the deposit impregnating technology condition and the technical parameter of optimization also are absolutely necessary, and this is the purpose of patent of the present invention.
Summary of the invention
The objective of the invention is to that separation and purification obtains a strain thiobacillus thiooxidans from the brass hill mouth copper mine soil sample of Hubei, be used to leach the microbe leaching-out method of low-grade phosphate ore.
A kind of thiobacillus thiooxidant of the present invention leaches phosphorus from low-grade phosphate ore method is achieved by following technical proposals: the bioleaching process of a kind of low-grade phosphate ore of the present invention comprises the steps:
1) sampling: the sampling jug and the sample scoop autoclave autoclaving that will tie with kraft paper, open kraft paper at sampling spot, burn bottleneck with spirit lamp, with sample scoop from Hubei brass hill mouth copper mine soil sampling to sampling jug 1/2nd, tying kraft paper rapidly takes back, take by weighing the 10g soil sample in the 100mL sterilized water, add magnetic stir bar and stirred 1 hour, leave standstill then;
2) enrichment, separation and purifying: draw above-mentioned supernatant liquor 10mL in the 250mL Erlenmeyer flask that fills the sterilized Waksman substratum of 100mL, in 30 ℃, the constant temperature shaking table of 120r/min, this process need 7d; Bacterium liquid 10mL after absorption is cultivated for the first time is in the 250mL Erlenmeyer flask that fills 100mL Waksman substratum, shaking culture once more under the situation identical with above-mentioned condition, shaking culture is 5 times so repeatedly, then bacterium liquid is adopted the separation of solid medium dilution spread plate method, purifying; The purifying bacterial classification small colonies about circular protrusions shape diameter 1mm occurs cultivating 7-10d on the Starkey solid medium, independent small colonies is provoked, be inoculated in the fine taper bottle or test tube that the 10mL liquid nutrient medium is housed, seal with gauze, culture condition is the same, and is evenly muddy up to the liquid of fine taper bottle or test tube; Repeat in the liquid medium within enlarged culturing and on flat board, separate repeatedly 4 times, isolate the purifying bacterial strain at last and carry out molecules again and identify and confirm as thiobacillus thiooxidans;
3) domestication: imbitition is cultivated bacterium liquid 10mL in the 250mL Erlenmeyer flask that fills the sterilized Waksman substratum of 90mL, add ground phosphate rock and each 1g of pyrite, shaking culture 10-15d in 30 ℃, the constant temperature shaking table of 120r/min, taking-up 10mL domestication liquid is forwarded in the fresh Wksman substratum to be cultivated, when the oyster white phenomenon appears in the culture medium solution color, illustrate that this bacterial classification can tolerate top pulp density and the wherein contained objectionable impurities that has, this process need is cultivated 5-10d; In former domestication liquid, add each 1g of ground phosphate rock and pyrite, repeat the above-mentioned steps shaking culture, when the oyster white phenomenon appears in solution colour once more, progressively increase ground phosphate rock again and pyrite is tamed repeatedly, add in the Erlenmeyer flask all to increase by 1 gram pyrite and phosphorus ore amount at every turn, carry out altogether 4 times; The volume that switching reduces in the former domestication liquid and the volume of evaporation do not have the Wksman culture medium supplemented of S with fresh no P;
Liquid nutrient medium adopts the Waksman substratum: (NH
4)
2SO
40.2g/L, K
2HPO
43H
2O 3.0g/L, MgSO
47H
2O 0.5g/L, CaCl
22H
2O 0.126g/L, S
010.0g/L distilled water 1000mL uses 50%H
2SO
4Adjust pH to 2.0.
Solid medium adopts Starky-Na
2S
2O
3-nutrient agar: (NH
4)
2SO
42.0g/L, MgSO
47H
2O 0.5g/L, CaCl
22H
2O 0.25g/L, KH
2PO
43.0g/L, FeSO
47H
2O0.001g/L, Na
2S
2O
310g/L, agar 3.0g/L, distilled water 1000mL;
Soak the ore deposit substratum: promptly the Waksman substratum does not add K
2HPO
43H
2O and S
0
4) soak the phosphorus method: in filling the 250mL Erlenmeyer flask that 100mL soaks the ore deposit substratum, the bacterial classification v/v that needs to insert the process domestication according to experiment, 20%～30%, adding a certain amount of pyrite 0.5g～2.0g and corresponding ore grinding time respectively is 10～20min levigated phosphorus ore 0.5g～2.0g, regulate initial pH1.0～2.5 with 1+1 sulfuric acid, in the constant temperature shaking table of 30 ℃ of design temperatures and rotating speed 120r/min, cultivated 7 days, get the 1mL leach liquor from Erlenmeyer flask, concentration with solubility total phosphorus in the yellow colorimetric method for determining solution of phosphorus molybdenum, according to the variation of phosphorus content in the solution before and after the experiment, the leaching yield of calculating phosphorus in the Rock Phosphate (72Min BPL) is 89.27%～95.67%.
A kind of thiobacillus thiooxidant of the present invention leaches phosphorus from low-grade phosphate ore method has following beneficial effect compared with prior art: the bacterial strain that the present invention's screening obtains not only soaks the phosphorus rate compared with the bacterial strain that occurs in the existing report and tests more than 30% apparently higher than other; and the condition that phosphorus ore leaches relatively meets actual production technology; selecting for use as initial pH is 2.0; the ore grinding time is selected 20min for use; soak phosphorus time 5d and shorten 5-10 days than other extraction time etc.; the present invention utilizes in sulphur in the pyrite (or ore self association) of adding and the phosphorus ore phosphorus as bacterial growth energy thing and nutrition; substitute sulphur and the element phosphor that needs adding in addition in the Waksman substratum respectively; the phosphorus compound a large amount of and expensive in the substratum and the adding of free sulfur have been reduced; the circulation and efficient utilization that help associated resources in the phosphorus ore; reduce environmental pollution; reduce cost of investment; production cost is lower, can realize the sustainable development of development of resources and environment protection.
Embodiment
The technical scheme that a kind of thiobacillus thiooxidant of the present invention is leached the method for phosphorus below in conjunction with embodiment from low-grade phosphate ore be further described as.
Thiobacillus thiooxidant of the present invention leaches phosphorus from low-grade phosphate ore method comprises the steps:
1) sampling: the sampling jug and the sample scoop autoclave autoclaving that will tie with kraft paper, open kraft paper at sampling spot, burn bottleneck with spirit lamp, with sample scoop from Hubei brass hill mouth copper mine soil sampling to sampling jug 1/2nd, tying kraft paper rapidly takes back, take by weighing the 10g soil sample in the 100mL sterilized water, add magnetic stir bar and stirred 1 hour, leave standstill then;
2) enrichment, separation and purifying: draw above-mentioned supernatant liquor 10mL in the 250mL Erlenmeyer flask that fills the sterilized Waksman substratum of 100mL, in 30 ℃, the constant temperature shaking table of 120r/min, this process need 7d.Bacterium liquid 10mL after absorption is cultivated for the first time is in the 250mL Erlenmeyer flask that fills 100mL Waksman substratum, shaking culture once more under the situation identical with above-mentioned condition, shaking culture is 5 times so repeatedly, then bacterium liquid is adopted the separation of solid medium dilution spread plate method, purifying.The purifying bacterial classification small colonies about circular protrusions shape diameter 1mm occurs cultivating 7-10d on the Starkey solid medium, independent small colonies is provoked, be inoculated in the fine taper bottle or test tube that the 10mL liquid nutrient medium is housed, seal with gauze, culture condition is the same, and is evenly muddy up to the liquid of fine taper bottle or test tube; Repeat in the liquid medium within enlarged culturing and on flat board, separate repeatedly 4 times, isolate strain excellent at last.
3) domestication: imbitition is cultivated bacterium liquid 10mL in the 250mL Erlenmeyer flask that fills the sterilized Waksman substratum of 90mL, add ground phosphate rock and each 1g of pyrite, shaking culture 10-15d in 30 ℃, the constant temperature shaking table of 120r/min, taking-up 10mL domestication liquid is forwarded in the fresh Wksman substratum to be cultivated, when the oyster white phenomenon appears in the culture medium solution color, illustrate that this bacterial classification can tolerate top pulp density and the wherein contained objectionable impurities that has, this process need is cultivated 5-10d.In former domestication liquid, add each 1g of ground phosphate rock and pyrite, repeat the above-mentioned steps shaking culture, when the oyster white phenomenon appears in solution colour once more, progressively increase ground phosphate rock and pyrite again and tame (all increasing by 1 gram) repeatedly at every turn, carry out altogether 4 times.The volume that switching reduces in the former domestication liquid and the volume of evaporation do not have the Wksman culture medium supplemented of S with fresh no P.
Liquid nutrient medium adopts the Waksman substratum: (NH
4)
2SO
40.2g/L, K
2HPO
43H
2O 3.0g/L, MgSO
47H
2O 0.5g/L, CaCl
22H
2O 0.126g/L, S
010.0g/L, distilled water 1000mL 50%H
2SO
4Adjust pH to 2.0.
Solid medium adopts Starky-Na
2S
2O
3-nutrient agar: (NH
4)
2SO
42.0g/L, MgSO
47H
2O 0.5g/L, CaCl
22H
2O 0.25g/L, KH
2PO
43.0g/L, FeSO
47H
2O0.001g/L, Na
2S
2O
310g/L, agar 3.0g/L, distilled water 1000mL.
Soak the ore deposit substratum: promptly the Waksman substratum does not add K
2HPO
43H
2O and S
0
4) soak the phosphorus method: in filling the 250mL Erlenmeyer flask that 100mL soaks the ore deposit substratum, bacterial classification (the v/v that needs to insert the process domestication according to experiment, 20%～30%), (0.5g～2.0g) and corresponding ore grinding time are (10～20min) levigated phosphorus ores (0.5g～2.0g) to add a certain amount of pyrite respectively, regulate initial pH (1.0～2.5) with 1+1 sulfuric acid, in the constant temperature shaking table of 30 ℃ of design temperatures and rotating speed 120r/min, cultivated 7 days, get the 1mL leach liquor from Erlenmeyer flask, concentration with solubility total phosphorus in the yellow colorimetric method for determining solution of phosphorus molybdenum, according to the variation of phosphorus content in the solution before and after the experiment, the leaching yield of calculating phosphorus in the Rock Phosphate (72Min BPL) is 89.27%～95.67%.
Described processing condition are:
Not having P at 100mL does not have sulphur Waksman and soaks in the substratum of ore deposit, insert the thiobacillus thiooxidant 30mL after taming, pH=2.0, the ore grinding time is that the ground phosphate rock add-on of 20min granularity is 1.0g, pyritous dosage is 1.0g, in temperature is 30 ℃, and rotating speed is to cultivate in the constant temperature shaking table of 120r/min to soak phosphorus 7d, and soaking the phosphorus rate is 94.37%.
Embodiment 1
The ore character of present embodiment is collophanite, contains P
2Be 21.98%, belong to low-grade, the chemical ingredients of other elements and content are (wt%): SiO in the phosphorus ore
224.94, Fe
2O
32.52 CaO 33.55, MgO 1.27, Al
2O
35.08 S 1.45, F 2.09 etc.; Main thing phase composite is (wt%) phosphatic rock+collophanite 53.00, pyrite 3.85, and carbonaceous 2.50, feldspar+quartz 30.50 in addition, also has calcite, rhombspar, bast etc.TFe38.77% in the pyrite (wt), granularity accounts for 90.2% for-200 orders.
The technical matters condition of present embodiment is: not having P at 100mL does not have sulphur Waksman and soaks in the substratum of ore deposit, insert the thiobacillus thiooxidant 20mL after taming, initial pH is 1.0, phosphorus ore concentration is 0.5g, granularity is ore grinding time 10min, and the pyrite add-on is 0.5g, and the setting rotating speed is 120r/min, temperature is to cultivate in 30 ℃ the constant temperature shaking table to leach 7d, and the phosphorus rate of soaking that records reaches 89.27%.
Embodiment 2
The pyrite of ore character, mineral composition and the use of present embodiment is identical with embodiment 1.
The technical matters condition of present embodiment is: not having P at 100mL does not have sulphur Waksman and soaks in the substratum of ore deposit, insert the thiobacillus thiooxidant 25mL after taming, initial pH is 2.0, phosphorus ore concentration is 2.0g, granularity is ore grinding time 20min, and the pyrite add-on is 1.0g, is 120r/min at rotating speed, temperature is to soak ore deposit 7d in 30 ℃ the constant temperature shaking table, soaks the phosphorus rate and reaches 90.71%.
Embodiment 3
The pyrite of ore character, mineral composition and the use of present embodiment is identical with embodiment 1.
The art processing condition of present embodiment are: not having P at 100mL does not have sulphur Waksman and soaks in the substratum of ore deposit, insert the thiobacillus thiooxidant 30mL after taming, pH=2.5, ore grinding time 15min, phosphorus ore concentration is 1.0g/L, and the pyrite add-on is 1.0g, is 30 ℃ in temperature, rotating speed is to cultivate in the constant temperature shaking table of 120r/min to soak phosphorus 7d, and soaking the phosphorus rate is 95.67%.
Embodiment 4.
The pyrite of ore character, mineral composition and the use of present embodiment is identical with embodiment 1.
The art processing condition of present embodiment are: not having P at 100mL does not have sulphur Waksman and soaks in the substratum of ore deposit, insert the thiobacillus thiooxidant 30mL after taming, pH=2.0, the ore grinding time is that the ground phosphate rock add-on of 20min granularity is 1.0g, and pyritous dosage is 1.0g, is 30 ℃ in temperature, rotating speed is to cultivate in the constant temperature shaking table of 120r/min to soak phosphorus 7d, and soaking the phosphorus rate is 94.37%.
Claims (3)
1. a thiobacillus thiooxidant leaches the method for phosphorus from low-grade phosphate ore, it is characterized in that described method comprises the steps:
1) sampling: the sampling jug and the sample scoop autoclave autoclaving that will tie with kraft paper, open kraft paper at sampling spot, burn bottleneck with spirit lamp, with sample scoop from Hubei brass hill mouth copper mine soil sampling to sampling jug 1/2nd, tying kraft paper rapidly takes back, take by weighing the 10g soil sample in the 100mL sterilized water, add magnetic stir bar and stirred 1 hour, leave standstill then;
2) enrichment, separation and purifying: draw above-mentioned supernatant liquor 10mL in the 250mL Erlenmeyer flask that fills the sterilized Waksman substratum of 100mL, in 30 ℃, the constant temperature shaking table of 120r/min, this process need 7d; Bacterium liquid 10mL after absorption is cultivated for the first time is in the 250mL Erlenmeyer flask that fills 100mL Waksman substratum, shaking culture once more under the situation identical with above-mentioned condition, shaking culture is 5 times so repeatedly, then bacterium liquid is adopted the separation of solid medium dilution spread plate method, purifying; The purifying bacterial classification small colonies about circular protrusions shape diameter 1mm occurs cultivating 7-10d on the Starkey solid medium, independent small colonies is provoked, be inoculated in the fine taper bottle or test tube that the 10mL liquid nutrient medium is housed, seal with gauze, culture condition is the same, and is evenly muddy up to the liquid of fine taper bottle or test tube; Repeat in the liquid medium within enlarged culturing and on flat board, separate repeatedly 4 times, isolate the purifying bacterial strain at last and carry out molecules again and identify and confirm as thiobacillus thiooxidans;
3) domestication: imbitition is cultivated bacterium liquid 10mL in the 250mL Erlenmeyer flask that fills the sterilized Waksman substratum of 90mL, add ground phosphate rock and each 1g of pyrite, shaking culture 10-15d in 30 ℃, the constant temperature shaking table of 120r/min, taking-up 10mL domestication liquid is forwarded in the fresh Wksman substratum to be cultivated, when the oyster white phenomenon appears in the culture medium solution color, illustrate that this bacterial classification can tolerate top pulp density and the wherein contained objectionable impurities that has, this process need is cultivated 5-10d; In former domestication liquid, add each 1g of ground phosphate rock and pyrite, repeat the above-mentioned steps shaking culture, when the oyster white phenomenon appears in solution colour once more, progressively increase ground phosphate rock again and pyrite is tamed repeatedly, add in the Erlenmeyer flask all to increase by 1 gram pyrite and phosphorus ore amount at every turn, carry out altogether 4 times; The volume that switching reduces in the former domestication liquid and the volume of evaporation do not have the Wksman culture medium supplemented of S with fresh no P;
Liquid nutrient medium adopts the Waksman substratum: (NH
4)
2SO
40.2g/L, K
2HPO
43H
2O 3.0g/L, MgSO
47H
2O 0.5g/L, CaCl
22H
2O 0.126g/L, S
010.0g/L, distilled water 1000mL.
Solid medium adopts Starky-Na
2S
2O
3-nutrient agar: (NH
4)
2SO
42.0g/L, MgSO
47H
2O 0.5g/L, CaCl
22H
2O 0.25g/L, KH
2PO
43.0g/L, FeSO
47H
2O0.001g/L, Na
2S
2O
310g/L, agar 3.0g/L, distilled water 1000mL;
Soak the ore deposit substratum: promptly the Waksman substratum does not add K
2HPO
43H
2O and S
0
4) soak the phosphorus method: in filling the 250mL Erlenmeyer flask that 100mL soaks the ore deposit substratum, the bacterial classification v/v that needs to insert the process domestication according to experiment, 20%～30%, adding a certain amount of pyrite 0.5g～2.0g and corresponding ore grinding time respectively is 10～20min levigated phosphorus ore 0.5g～2.0g, regulate initial pH1.0～2.5 with 1+1 sulfuric acid, in the constant temperature shaking table of 30 ℃ of design temperatures and rotating speed 120r/min, cultivated 7 days, get the 1mL leach liquor from Erlenmeyer flask, concentration with solubility total phosphorus in the yellow colorimetric method for determining solution of phosphorus molybdenum, variation according to phosphorus content in the solution before and after the experiment, calculate the leaching yield of phosphorus in the Rock Phosphate (72Min BPL), 7 days soak the phosphorus rate is 89.27～95.67%.
2. thiobacillus thiooxidant according to claim 1 leaches the method for phosphorus from low-grade phosphate ore, it is characterized in that: described inoculum size 30% is meant that the bacterium liquid with 30mL is inoculated into soaking in the substratum of ore deposit of 100mL; The described ore deposit substratum that soaks: promptly the Waksman substratum does not add K
2HPO
43H
2O and S
0
3. thiobacillus thiooxidant according to claim 1 leaches the method for phosphorus from low-grade phosphate ore, it is characterized in that described processing condition are: not having P at 100mL does not have sulphur Waksman and soaks in the substratum of ore deposit, insert the thiobacillus thiooxidant 30mL after taming, pH=2.0, the ore grinding time is that the ground phosphate rock add-on of 20min granularity is 1.0g, pyritous dosage is 1.0g, in temperature is 30 ℃, and rotating speed is to cultivate in the constant temperature shaking table of 120r/min to soak phosphorus 7d, and soaking the phosphorus rate is 94.37%.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102173399A (en) * | 2011-03-08 | 2011-09-07 | 武汉工程大学 | Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile |
| CN102432363A (en) * | 2011-09-09 | 2012-05-02 | 西南科技大学 | Method for preparing bacterial composite phosphate fertilizer with medium and low grade phosphate ores |
| CN105502318A (en) * | 2016-01-29 | 2016-04-20 | 武汉工程大学 | Method for dissolving low- and medium-grade phosphorite by utilizing phosphorite pit water microbial flora |
| CN108753615A (en) * | 2018-05-24 | 2018-11-06 | 江口县旭辉生态农业科技有限公司 | A method of dissolving low grade rock phosphate using Haloragidaceae Myriophyllum spicatum endophyte |
| CN109517874A (en) * | 2018-11-27 | 2019-03-26 | 湖北中烟工业有限责任公司 | A kind of antibacterial agent screening technique extending cigarette berries extract shelf life |
| CN110624949A (en) * | 2019-09-30 | 2019-12-31 | 武汉工程大学 | Method for repairing excessive phosphorus pollution of phosphorite waste land by combining indigenous microorganisms and plants |
| CN114230023A (en) * | 2021-12-21 | 2022-03-25 | 常州纺织服装职业技术学院 | Method for treating sulfur-containing solid waste by microorganisms |
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2009
- 2009-12-01 CN CN200910216639A patent/CN101837962A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102173399A (en) * | 2011-03-08 | 2011-09-07 | 武汉工程大学 | Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile |
| CN102173399B (en) * | 2011-03-08 | 2012-11-21 | 武汉工程大学 | Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile |
| CN102432363A (en) * | 2011-09-09 | 2012-05-02 | 西南科技大学 | Method for preparing bacterial composite phosphate fertilizer with medium and low grade phosphate ores |
| CN102432363B (en) * | 2011-09-09 | 2013-07-17 | 西南科技大学 | Method for preparing bacterial composite phosphate fertilizer with medium and low grade phosphate ores |
| CN105502318A (en) * | 2016-01-29 | 2016-04-20 | 武汉工程大学 | Method for dissolving low- and medium-grade phosphorite by utilizing phosphorite pit water microbial flora |
| CN108753615A (en) * | 2018-05-24 | 2018-11-06 | 江口县旭辉生态农业科技有限公司 | A method of dissolving low grade rock phosphate using Haloragidaceae Myriophyllum spicatum endophyte |
| CN109517874A (en) * | 2018-11-27 | 2019-03-26 | 湖北中烟工业有限责任公司 | A kind of antibacterial agent screening technique extending cigarette berries extract shelf life |
| CN110624949A (en) * | 2019-09-30 | 2019-12-31 | 武汉工程大学 | Method for repairing excessive phosphorus pollution of phosphorite waste land by combining indigenous microorganisms and plants |
| CN110624949B (en) * | 2019-09-30 | 2021-10-12 | 武汉工程大学 | Method for repairing excessive phosphorus pollution of phosphorite waste land by combining indigenous microorganisms and plants |
| CN114230023A (en) * | 2021-12-21 | 2022-03-25 | 常州纺织服装职业技术学院 | Method for treating sulfur-containing solid waste by microorganisms |
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Application publication date: 20100922 |