CN114230023A - Method for treating sulfur-containing solid waste by microorganisms - Google Patents
Method for treating sulfur-containing solid waste by microorganisms Download PDFInfo
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- CN114230023A CN114230023A CN202111568969.5A CN202111568969A CN114230023A CN 114230023 A CN114230023 A CN 114230023A CN 202111568969 A CN202111568969 A CN 202111568969A CN 114230023 A CN114230023 A CN 114230023A
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- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 title claims abstract description 125
- 229910052717 sulfur Inorganic materials 0.000 title claims abstract description 125
- 239000011593 sulfur Substances 0.000 title claims abstract description 125
- 239000002910 solid waste Substances 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 34
- 244000005700 microbiome Species 0.000 title claims abstract description 23
- 241000605222 Acidithiobacillus ferrooxidans Species 0.000 claims abstract description 36
- 241000605272 Acidithiobacillus thiooxidans Species 0.000 claims abstract description 35
- 230000015556 catabolic process Effects 0.000 claims abstract description 32
- 238000006731 degradation reaction Methods 0.000 claims abstract description 32
- 239000007788 liquid Substances 0.000 claims description 99
- 230000001580 bacterial effect Effects 0.000 claims description 96
- 239000001963 growth medium Substances 0.000 claims description 35
- 239000002245 particle Substances 0.000 claims description 27
- 238000002156 mixing Methods 0.000 claims description 24
- 238000003756 stirring Methods 0.000 claims description 20
- 238000006477 desulfuration reaction Methods 0.000 claims description 15
- 230000023556 desulfurization Effects 0.000 claims description 15
- 238000011282 treatment Methods 0.000 claims description 11
- 239000012153 distilled water Substances 0.000 claims description 9
- 239000010802 sludge Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 238000012216 screening Methods 0.000 claims description 8
- 238000012258 culturing Methods 0.000 claims description 7
- 238000002360 preparation method Methods 0.000 claims description 7
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 6
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 229910052564 epsomite Inorganic materials 0.000 claims description 3
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 3
- 229910052603 melanterite Inorganic materials 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 238000012546 transfer Methods 0.000 claims description 3
- 230000000813 microbial effect Effects 0.000 claims 4
- 230000002906 microbiologic effect Effects 0.000 claims 3
- 239000000463 material Substances 0.000 claims 2
- 230000001590 oxidative effect Effects 0.000 abstract description 4
- 230000000593 degrading effect Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 239000007787 solid Substances 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 2
- 239000010920 waste tyre Substances 0.000 description 22
- 238000006243 chemical reaction Methods 0.000 description 13
- 239000002699 waste material Substances 0.000 description 8
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000000354 decomposition reaction Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 238000003916 acid precipitation Methods 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 238000009388 chemical precipitation Methods 0.000 description 1
- 238000002485 combustion reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000003546 flue gas Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000005342 ion exchange Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 230000033116 oxidation-reduction process Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/345—Biological treatment of water, waste water, or sewage characterised by the microorganisms used for biological oxidation or reduction of sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/02—Aerobic processes
- C02F3/12—Activated sludge processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/346—Iron bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/10—Biological treatment of water, waste water, or sewage
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
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- Processing Of Solid Wastes (AREA)
Abstract
The invention relates to a method for treating sulfur-containing solid waste by microorganisms, wherein Fe is added into thiobacillus ferrooxidans in the growth process2+Is directly oxidized to generate Fe3+Followed by Fe having strong oxidizing property3+Continuously oxidizing low-valence sulfur in sulfur-containing solid waste into SO4 2‑SO as to realize the degradation of the solid sulfur in the sulfur-containing solid waste, and the thiobacillus thiooxidans can directly oxidize the low-valence sulfur in the sulfur-containing solid waste into high-valence SO4 2‑The synergistic effect of the thiobacillus thiooxidans and the thiobacillus ferrooxidans can ensure that the degradation rate of sulfur in the sulfur-containing solid waste reaches 94 percent, the effect of degrading the sulfur-containing solid waste is obvious, and the method for treating the sulfur-containing solid waste is compared with methods such as an incineration method, a landfill method and the likeIs more environment-friendly and does not produce secondary pollution to the environment.
Description
Technical Field
The invention belongs to the technical field of waste treatment, and particularly relates to a method for treating sulfur-containing solid waste by using microorganisms.
Background
At present, the disposal of the sulfur-containing solid waste mainly comprises a burning method, a landfill method and a physical and chemical method. SO is generated in combustion flue gas of sulfur-containing solid wastes2，SO2Can cause the problems of air pollution, acid rain and the like, and further corrode vegetation, buildings and the like. With SO2Also can cause pneumonia, bronchitis and SO2Is inhaled by human bodyCan cause toxic effect on various tissues and organs along with blood. The tail gas requires additional treatment. The landfill method has longer degradation period and is easy to pollute soil. The physical and chemical treatments mainly include neutralization, oxidation-reduction, dissolution, chemical precipitation, adsorption, ion exchange, etc. The resulting wastewater requires additional treatment.
Disclosure of Invention
The technical problem to be solved by the invention is as follows: provides a method for treating sulfur-containing solid waste by microorganisms, which can effectively degrade the sulfur-containing solid waste.
The technical scheme adopted by the invention for solving the technical problems is as follows: a method for treating sulfur-containing solid waste by microorganisms comprises the following steps:
screening aerobic microorganisms from the activated sludge and carrying out enrichment culture to generate a desulfurization bacterial liquid, wherein the aerobic microorganisms comprise thiobacillus ferrooxidans and thiobacillus thiooxidans, and the desulfurization bacterial liquid is thiobacillus ferrooxidans bacterial liquid or thiobacillus thiooxidans bacterial liquid or a mixture of the thiobacillus ferrooxidans bacterial liquid and the thiobacillus thiooxidans bacterial liquid;
crushing the sulfur-containing solid waste into sulfur-containing solid waste particles;
mixing the desulfurization bacterial liquid and the crushed sulfur-containing solid waste particles according to a certain proportion, wherein the mixing proportion of the sulfur-containing solid waste is 10-30%, and then stirring for 2-15 days at the stirring speed of 75-90 r/min at the temperature of 25-35 ℃;
and (4) collecting the desulfurized sulfur-containing solid waste particles, and detecting the degradation rate of the sulfur-containing solid waste particles.
Further, the screening steps of the thiobacillus ferrooxidans are as follows:
adding 80ml of 9K culture medium and 20ml of activated sludge into 10 250ml conical flasks respectively, then putting 10 conical flasks into a constant-temperature air shaking incubator, adjusting the rotation speed to be 120r/min-200r/min, the temperature to be 20-40 ℃, taking out the conical flasks after 5 days, measuring the pH value of the internal bacterial liquid, taking out 10% of bacterial liquid from the conical flasks with the pH value lower than 5 respectively, transferring the bacterial liquid into new 9K culture medium respectively, culturing for a period of time, transferring 10% of the bacterial liquid in the culture medium to the new 9K culture medium, repeating the transfer for seven times until the color of the bacterial liquid in the culture medium is dark brown and the pH value is within the range of 1.8-2.2, determining 16S rRNA of microorganisms in the screened bacterial liquid, determining thiobacillus ferrooxidans, finally enriching and culturing the thiobacillus ferrooxidans, and forming a thiobacillus ferrooxidans bacterial liquid for later use.
Further, the thiobacillus thiooxidans screening steps are as follows:
adding 180ml of Waksman culture medium and 20ml of activated sludge into 10 250ml conical flasks respectively, putting the conical flasks into a constant-temperature air shaking incubator, adjusting the rotation speed to be 60r/min-100r/min, preferably 80r/min, the temperature to be 25-30 ℃, preferably 28 ℃, taking out the conical flasks after 6 days, measuring the pH of bacterial liquid in the conical flasks, transferring the bacterial liquid in the conical flasks of which the pH is 10% or less to a new Waksman culture medium, culturing for a period of time, transferring 10% of the bacterial liquid in the culture medium to the new Waksman culture medium until the bacterial liquid is in a uniform light yellow suspension state, measuring 16S rRNA of microorganisms in the screened bacterial liquid, identifying the rRNA as thiobacillus thiooxidans, and finally carrying out enrichment culture on the screened thiobacillus thiooxidans to form a thiooxidans bacterial liquid for later use.
Further, the 9K culture medium comprises a solution A and a solution B, and the steps for preparing the 9K culture medium are as follows: preparation of solution A: 3.0g (NH)4)2SO4、0.1gKCl、0.15gK2HPO4、0.5gMgSO4·7H2O and 0.01gCa (NO)3)2Dissolved in 700ml of distilled water and then treated with 5mol/L H2SO4Adjusting the pH to 2.0; and (3) preparation of a liquid B: 44.2g of FeSO4·7H2O is dissolved in 300ml of distilled water and then mixed with 5mol/L H2SO4Adjusting the pH to 2.0; finally, the solution A and the solution B are sterilized respectively and then mixed in a 1000ml Erlenmeyer flask for later use.
Further, the preparation of the Waksman medium was as follows: 0.2g of (NH)4)2SO4、3.00g K2HPO4、0.50g MgSO4·7H2O, 10g Sulfur and 0.25g CaCl2·2H2O is dissolved in 1000mL of distilled water and then mixed with 5mol/LH2SO4Adjusting pH to 4.00 for use.
Further, the temperature of the sulfur-containing solid waste and the desulfurization bacterial liquid is 30 ℃.
Further, the stirring speed of the sulfur-containing solid waste and the desulfurization bacterial liquid is 80 r/min.
Further, the stirring time of the sulfur-containing solid waste and the desulfurization bacterial liquid is 14 days.
Further, the mixing ratio of the sulfur-containing solid waste is 15%.
Further, the particle size of the sulfur-containing solid waste particles is 1000-1500 μm.
The invention has the beneficial effects that:
the invention relates to a method for treating sulfur-containing solid waste by microorganisms, wherein Fe is added into thiobacillus ferrooxidans in the growth process2+Is directly oxidized to generate Fe3+Followed by Fe having strong oxidizing property3+Continuously oxidizing low-valence sulfur in sulfur-containing solid waste into SO4 2-SO as to realize the degradation of the solid sulfur in the sulfur-containing solid waste, and the thiobacillus thiooxidans can directly oxidize the low-valence sulfur in the sulfur-containing solid waste into high-valence SO4 2-The synergistic effect of the thiobacillus thiooxidans and the thiobacillus ferrooxidans can ensure that the degradation rate of sulfur in the sulfur-containing solid waste reaches 94 percent, the effect of degrading the sulfur-containing solid waste is obvious, and compared with methods such as an incineration method and a landfill method, the method for treating the sulfur-containing solid waste is more environment-friendly and does not generate secondary pollution to the environment.
Drawings
The invention is further illustrated by the following figures and examples.
FIG. 1 is a graph showing the trend of the sulfur decomposition rate of Thiobacillus ferrooxidans, Thiobacillus thiooxidans and a mixed bacterium of Thiobacillus ferrooxidans and Thiobacillus thiooxidans with time;
FIG. 2 is a graph showing the relationship between sulfur-containing waste tires of different mixing ratios and the sulfur decomposition rate.
Detailed Description
The present invention will now be described in detail with reference to the accompanying drawings. This figure is a simplified schematic diagram, and merely illustrates the basic structure of the present invention in a schematic manner, and therefore it shows only the constitution related to the present invention.
The invention provides a method for treating sulfur-containing solid waste by microorganisms, which comprises the following steps:
screening aerobic microorganisms from the activated sludge and carrying out enrichment culture to generate a desulfurization bacterial liquid, wherein the aerobic microorganisms comprise thiobacillus ferrooxidans and thiobacillus thiooxidans, the desulfurization bacterial liquid can be any one of a thiobacillus ferrooxidans bacterial liquid or a thiobacillus thiooxidans bacterial liquid, and can also be a mixture of the thiobacillus ferrooxidans bacterial liquid and the thiobacillus thiooxidans bacterial liquid;
the screening steps of the thiobacillus ferrooxidans are as follows:
firstly, preparing a 9K culture medium, wherein the 9K culture medium comprises a solution A and a solution B, and the preparation of the solution A: 3.0g (NH)4)2SO4、0.1gKCl、0.15gK2HPO4、0.5gMgSO4·7H2O and 0.01gCa (NO)3)2Dissolved in 700ml of distilled water and then treated with 5mol/L H2SO4Adjusting the pH to 2.0; and (3) preparation of a liquid B: 44.2g of FeSO4·7H2O is dissolved in 300ml of distilled water and then mixed with 5mol/L H2SO4Adjusting the pH to 2.0; finally, the solution A and the solution B are sterilized respectively and then mixed in a 1000ml Erlenmeyer flask for later use.
Then adding 80ml of 9K culture medium and 20ml of activated sludge into 10 250ml conical flasks respectively, then putting 10 conical flasks into a constant-temperature air shaking incubator, adjusting the rotation speed to be 120r/min-200r/min, preferably 160r/min, the temperature to be 20 ℃ -40 ℃, preferably 30 ℃, taking out the conical flasks after 5 days, measuring the pH value of the internal bacterial liquid, taking out 10% bacterial liquid from the conical flasks with the pH value lower than 5 respectively, then transferring the bacterial liquid into a new 9K culture medium respectively, after a period of culture, transferring 10% of the bacterial liquid in the culture medium to the new 9K culture medium, repeating the transfer for seven times until the color of the bacterial liquid in the culture medium is dark brown and the pH value is within the range of 1.8-2.2, then measuring 16S rRNA of microorganisms in the screened bacterial liquid, identifying the microorganisms as thiobacillus ferrooxidans, finally screening the thiobacillus ferrooxidans for enrichment culture, and forming a thiobacillus ferrooxidans bacterial liquid for later use.
The thiobacillus thiooxidans screening procedure is as follows:
firstly, preparing a Waksman liquid culture medium, comprising the following steps: 0.2g of (NH)4)2SO4、3.00g K2HPO4、0.50g MgSO4·7H2O, 10g Sulfur and 0.25g CaCl2·2H2O is dissolved in 1000mL of distilled water and then mixed with 5mol/LH2SO4Adjusting pH to 4.00 for use.
Then, respectively adding 180ml of Waksman culture medium and 20ml of activated sludge into 10 250ml conical flasks, putting the conical flasks into a constant-temperature air shaking incubator, adjusting the rotation speed to be 60r/min-100r/min, preferably 80r/min, the temperature to be 25-30 ℃, preferably 28 ℃, taking out the conical flasks after 6 days, measuring the pH of bacterial liquid in the conical flasks, transferring the bacterial liquid in the conical flasks of which the pH is 10% or less to a new Waksman culture medium, culturing for a period of time, transferring 10% of the bacterial liquid in the culture medium to the new Waksman culture medium until the bacterial liquid is in a uniform light yellow suspension state, then measuring 16S rRNA of microorganisms in the screened bacterial liquid, identifying the bacterial liquid as thiobacillus thiooxidans, and finally carrying out enrichment culture on the screened thiobacillus thiooxidans to form a thiobacillus thiooxidans bacterial liquid for later use.
And (2) crushing the sulfur-containing solid waste, and sorting the crushed sulfur-containing solid waste by using a sieve of 80-120 meshes, preferably 100 meshes to obtain particles with the particle size of 1000-1500 mu m for later use.
And (3) mixing the desulfurization bacterial liquid and the crushed sulfur-containing solid waste particles according to a certain proportion, wherein the mixing proportion of the sulfur-containing solid waste is 10-30%, preferably 15%, and then stirring for 2-15 days, preferably 14 days, at the temperature of 25-35 ℃, preferably 30 ℃ and at the stirring speed of 75-90 r/min, preferably 80 r/min.
And (4) collecting the desulfurized sulfur-containing solid waste, and detecting the degradation rate of the desulfurized sulfur-containing solid waste, wherein the degradation rate is obtained by sequentially detecting the sulfur content of the sulfur-containing solid waste before desulfurization and the sulfur content of the desulfurized sulfur-containing solid waste through a sulfur detector and calculating.
Example 1
85ml of the thiobacillus ferrooxidans bacterial liquid and 15g of the sulfur-containing waste tire particles are sequentially added into a reaction vessel, wherein the mixing proportion of the sulfur-containing waste tire is 15%, the stirring speed is adjusted to 80r/min, the temperature is controlled to be 30 ℃, the reaction lasts for 14 days, and then the degradation rate of sulfur in the waste tire particles is monitored, and the result is shown in figure 1.
Example 2
Adding 85ml of thiobacillus thiooxidans bacterial liquid and 15g of sulfur-containing waste tire particles into a reaction container in sequence, wherein the mixing proportion of the sulfur-containing waste tire is 15%, adjusting the stirring speed to 80r/min, controlling the temperature to be 30 ℃, reacting for 14 days, and then monitoring the degradation rate of sulfur in the waste tire particles, wherein the result is shown in figure 1.
Example 3
Adding 85ml of a mixed bacterial liquid of thiobacillus ferrooxidans bacterial liquid and thiobacillus thiooxidans bacterial liquid and 15g of sulfur-containing waste tire particles into a reaction container in sequence, wherein the mixing proportion of the sulfur-containing waste tire is 15%, the stirring speed is adjusted to 80r/min, the temperature is controlled to be 30 ℃, the reaction lasts for 14 days, and then monitoring the degradation rate of sulfur in the waste tire particles, and the result is shown in figure 1.
As shown in fig. 1, in examples 1 to 3, the degradation rate of sulfur increased as the stirring time increased, the degradation rate of sulfur increased more slowly in the interval of 0 to 4, the degradation rate of sulfur increased rapidly in the interval of 4 to 10, the trend of the increase of the degradation rate of sulfur in the interval of 10 to 14 gradually decreased and tended to be gentle, the degradation rate of sulfur reached the maximum on day 14, and the degradation rate of sulfur in the mixed bacterial liquid was always greater than the degradation rate of sulfur in the thiobacillus ferrooxidans bacterial liquid and the degradation rate of sulfur in the thiobacillus thiooxidans bacterial liquid in the same period of time, so that it was found that the mixed bacterial liquid had the highest degradation rate.
Example 4
Adding 95ml of a mixed bacterial liquid of the thiobacillus ferrooxidans bacterial liquid and the thiobacillus thiooxidans bacterial liquid and 5g of sulfur-containing waste tire particles into a reaction container in sequence, wherein the mixing proportion of the sulfur-containing waste tires is 5%, adjusting the stirring speed to 80r/min, controlling the temperature to be 30 ℃, reacting for 14 days, and finally measuring the degradation rate of sulfur, wherein the result is shown in figure 2.
Example 5
90ml of a mixed bacterial liquid of the thiobacillus ferrooxidans bacterial liquid and the thiobacillus thiooxidans bacterial liquid and 10g of sulfur-containing waste tire particles are sequentially added into a reaction vessel, wherein the mixing proportion of the sulfur-containing waste tires is 10%, the stirring speed is adjusted to 80r/min, the temperature is controlled to be 30 ℃, the reaction lasts for 14 days, and finally the degradation rate of sulfur is measured, and the result is shown in figure 2.
Example 6
Adding 85ml of mixed bacterial liquid of the thiobacillus ferrooxidans bacterial liquid and the thiobacillus thiooxidans bacterial liquid and 15g of sulfur-containing waste tire particles into a reaction container in sequence, wherein the mixing proportion of the sulfur-containing waste tires is 15%, adjusting the stirring speed to 80r/min, controlling the temperature to be 30 ℃, reacting for 14 days, and finally measuring the degradation rate of sulfur, wherein the result is shown in figure 2.
Example 7
Adding 80ml of a mixed bacterial liquid of the thiobacillus ferrooxidans bacterial liquid and the thiobacillus thiooxidans bacterial liquid and 20g of sulfur-containing waste tire particles into a reaction container in sequence, wherein the mixing proportion of the sulfur-containing waste tires is 20%, adjusting the stirring speed to 80r/min, controlling the temperature to be 30 ℃, reacting for 14 days, and finally measuring the degradation rate of sulfur, wherein the result is shown in figure 2.
Example 8
Adding 75ml of a mixed bacterial liquid of the thiobacillus ferrooxidans bacterial liquid and the thiobacillus thiooxidans bacterial liquid and 25g of sulfur-containing waste tire particles into a reaction container in sequence, wherein the mixing proportion of the sulfur-containing waste tires is 25%, adjusting the stirring speed to 80r/min, controlling the temperature to be 30 ℃, reacting for 14 days, and finally measuring the degradation rate of sulfur, wherein the result is shown in figure 2.
Example 9
70ml of a mixed bacterial liquid of the thiobacillus ferrooxidans bacterial liquid and the thiobacillus thiooxidans bacterial liquid and 30g of sulfur-containing waste tire particles are sequentially added into a reaction vessel, wherein the mixing proportion of the sulfur-containing waste tires is 30%, the stirring speed is adjusted to 80r/min, the temperature is controlled to be 30 ℃, the reaction lasts for 14 days, and finally the degradation rate of sulfur is measured, and the result is shown in figure 2.
As shown in fig. 2, in examples 4 to 9, the degradation rate of sulfur gradually decreased as the mixing ratio of the sulfur-containing waste increased, and the degradation rate of sulfur tended to decrease slowly when the mixing ratio of the sulfur-containing waste increased from 5% to 15%, and the degradation rate of sulfur decreased greatly when the mixing ratio of the sulfur-containing waste increased from 15% to 30%, and the degradation rate of sulfur was not the highest when the mixing ratio of the sulfur-containing waste was 15%, but from the viewpoint of overall efficiency, the degradation rate reached 94% when the mixing ratio of the sulfur-containing waste was 15%, and relatively large amounts of sulfur-containing waste were degraded, and thus it was found that the advantage was the best when the mixing ratio of the sulfur-containing waste was 15%.
In light of the foregoing description of preferred embodiments in accordance with the invention, it is to be understood that numerous changes and modifications may be made by those skilled in the art without departing from the scope of the invention. The technical scope of the present invention is not limited to the contents of the specification, and must be determined according to the scope of the claims.
Claims (10)
1. A method for treating sulfur-containing solid waste by microorganisms comprises the following steps:
screening aerobic microorganisms from the activated sludge and carrying out enrichment culture to generate a desulfurization bacterial liquid, wherein the aerobic microorganisms comprise thiobacillus ferrooxidans and thiobacillus thiooxidans, and the desulfurization bacterial liquid is thiobacillus ferrooxidans bacterial liquid or thiobacillus thiooxidans bacterial liquid or a mixture of the thiobacillus ferrooxidans bacterial liquid and the thiobacillus thiooxidans bacterial liquid;
crushing the sulfur-containing solid waste into sulfur-containing solid waste particles;
mixing the desulfurization bacterial liquid and the crushed sulfur-containing solid waste particles according to a certain proportion, wherein the mixing proportion of the sulfur-containing solid waste is 10-30%, and then stirring for 2-15 days at the stirring speed of 75-90 r/min at the temperature of 25-35 ℃;
and (4) collecting the desulfurized sulfur-containing solid waste particles, and detecting the degradation rate of the sulfur-containing solid waste particles.
2. The method for the microbiological treatment of sulfur-containing solid waste material as claimed in claim 1, wherein said Thiobacillus ferrooxidans is selected by the following steps:
adding 80ml of 9K culture medium and 20ml of activated sludge into 10 250ml conical flasks respectively, then putting 10 conical flasks into a constant-temperature air shaking incubator, adjusting the rotation speed to be 120r/min-200r/min, the temperature to be 20-40 ℃, taking out the conical flasks after 5 days, measuring the pH value of the internal bacterial liquid, taking out 10% of bacterial liquid from the conical flasks with the pH value lower than 5 respectively, transferring the bacterial liquid into new 9K culture medium respectively, culturing for a period of time, transferring 10% of the bacterial liquid in the culture medium to the new 9K culture medium, repeating the transfer for seven times until the color of the bacterial liquid in the culture medium is dark brown and the pH value is within the range of 1.8-2.2, determining 16S rRNA of microorganisms in the screened bacterial liquid, determining thiobacillus ferrooxidans, finally enriching and culturing the thiobacillus ferrooxidans, and forming a thiobacillus ferrooxidans bacterial liquid for later use.
3. The method for the microbiological treatment of sulfur-containing solid waste as claimed in claim 1, wherein the thiobacillus thiooxidans is selected by the following steps:
adding 180ml of Waksman culture medium and 20ml of activated sludge into 10 250ml conical flasks respectively, putting the conical flasks into a constant-temperature air shaking incubator, adjusting the rotation speed to be 60r/min-100r/min, the temperature to be 25-30 ℃, taking out the conical flasks after 6 days, measuring the pH of bacterial liquid in the conical flasks, taking bacterial liquid in the conical flasks of which the pH is reduced to be below 2.0, transferring the bacterial liquid into a new Waksman culture medium, culturing for a period of time, transferring 10% of the bacterial liquid in the culture medium into the new Waksman culture medium until the bacterial liquid is in a uniform light yellow suspension state, measuring 16S rRNA of microorganisms in the screened bacterial liquid, identifying the bacterial liquid as thiobacillus thiooxidans, and finally carrying out enrichment culture on the screened thiobacillus thiooxidans to form a standby bacterial liquid of the thiobacillus thiooxidans.
4. A method of treating sulfur-containing solid waste by microorganisms according to claim 2The method of (3), wherein the 9K culture medium comprises solution A and solution B, and the steps for preparing the 9K culture medium are as follows: preparation of solution A: 3.0g (NH)4)2SO4、0.1gKCl、0.15gK2HPO4、0.5gMgSO4·7H2O and 0.01gCa (NO)3)2Dissolved in 700ml of distilled water and then treated with 5mol/L H2SO4Adjusting the pH to 2.0; and (3) preparation of a liquid B: 44.2g of FeSO4·7H2O is dissolved in 300ml of distilled water and then mixed with 5mol/L H2SO4Adjusting the pH to 2.0; finally, the solution A and the solution B are sterilized respectively and then mixed in a 1000ml Erlenmeyer flask for later use.
5. The method for the microbial treatment of sulfur-containing solid waste according to claim 3, wherein the Waksman culture medium is prepared by the following steps: 0.2g of (NH)4)2SO4、3.00g K2HPO4、0.50g MgSO4·7H2O, 10g Sulfur and 0.25g CaCl2·2H2O is dissolved in 1000mL of distilled water and then mixed with 5mol/LH2SO4Adjusting pH to 4.00 for use.
6. The method of claim 1, wherein the temperature of the sulfur-containing solid waste and the temperature of the desulfurized bacterial liquid are 30 ℃.
7. The method for the microbial treatment of sulfur-containing solid waste as claimed in claim 1, wherein the stirring speed of the sulfur-containing solid waste and the desulfurization bacterial liquid is 80 r/min.
8. The method for the microbial treatment of sulfur-containing solid waste as claimed in claim 1, wherein the stirring time of the sulfur-containing solid waste and the desulfurization bacterial liquid is 14 days.
9. The method for the microbial treatment of sulfur-containing solid waste as claimed in claim 1, wherein the mixing ratio of said sulfur-containing solid waste is 15%.
10. The method for the microbiological treatment of sulfur-containing solid waste material as claimed in claim 1, wherein said sulfur-containing solid waste particles have a particle size of 1000 μm to 1500 μm.
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