CN107118982A - A kind of Thiobacillus ferrooxidans and its application - Google Patents
A kind of Thiobacillus ferrooxidans and its application Download PDFInfo
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- CN107118982A CN107118982A CN201710312729.6A CN201710312729A CN107118982A CN 107118982 A CN107118982 A CN 107118982A CN 201710312729 A CN201710312729 A CN 201710312729A CN 107118982 A CN107118982 A CN 107118982A
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- 241000605222 Acidithiobacillus ferrooxidans Species 0.000 title claims abstract description 21
- 239000007789 gas Substances 0.000 claims abstract description 16
- 230000001580 bacterial effect Effects 0.000 claims abstract description 11
- 239000007788 liquid Substances 0.000 claims description 52
- 239000001963 growth medium Substances 0.000 claims description 18
- 241000894006 Bacteria Species 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 239000012153 distilled water Substances 0.000 claims description 13
- 238000000034 method Methods 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 7
- 239000007787 solid Substances 0.000 claims description 7
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 6
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 6
- 229910052564 epsomite Inorganic materials 0.000 claims description 6
- 229910052603 melanterite Inorganic materials 0.000 claims description 6
- 229920000936 Agarose Polymers 0.000 claims description 5
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 5
- 230000001954 sterilising effect Effects 0.000 claims description 5
- 238000004659 sterilization and disinfection Methods 0.000 claims description 5
- 239000000945 filler Substances 0.000 claims description 4
- 239000002773 nucleotide Substances 0.000 claims description 4
- 125000003729 nucleotide group Chemical group 0.000 claims description 4
- 239000005864 Sulphur Substances 0.000 claims description 3
- 238000001816 cooling Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 238000012216 screening Methods 0.000 claims description 3
- 108020004465 16S ribosomal RNA Proteins 0.000 claims description 2
- 239000008246 gaseous mixture Substances 0.000 claims description 2
- 238000009629 microbiological culture Methods 0.000 claims description 2
- 238000004821 distillation Methods 0.000 claims 2
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims 1
- 239000011248 coating agent Substances 0.000 claims 1
- 238000000576 coating method Methods 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 abstract description 19
- 229910000037 hydrogen sulfide Inorganic materials 0.000 abstract description 19
- 238000006477 desulfuration reaction Methods 0.000 abstract description 5
- 230000023556 desulfurization Effects 0.000 abstract description 4
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 4
- 238000000746 purification Methods 0.000 abstract 1
- 239000010865 sewage Substances 0.000 abstract 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 5
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 5
- 230000003647 oxidation Effects 0.000 description 5
- 238000007254 oxidation reaction Methods 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000002054 inoculum Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000009423 ventilation Methods 0.000 description 3
- 241000266272 Acidithiobacillus Species 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000001822 immobilized cell Anatomy 0.000 description 2
- 229910000474 mercury oxide Inorganic materials 0.000 description 2
- UKWHYYKOEPRTIC-UHFFFAOYSA-N mercury(ii) oxide Chemical compound [Hg]=O UKWHYYKOEPRTIC-UHFFFAOYSA-N 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000007493 shaping process Methods 0.000 description 2
- 238000011282 treatment Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 229910001448 ferrous ion Inorganic materials 0.000 description 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- -1 iron ion Chemical class 0.000 description 1
- 229910000360 iron(III) sulfate Inorganic materials 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000003345 natural gas Substances 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/46—Removing components of defined structure
- B01D53/48—Sulfur compounds
- B01D53/52—Hydrogen sulfide
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D53/00—Separation of gases or vapours; Recovering vapours of volatile solvents from gases; Chemical or biological purification of waste gases, e.g. engine exhaust gases, smoke, fumes, flue gases, aerosols
- B01D53/34—Chemical or biological purification of waste gases
- B01D53/74—General processes for purification of waste gases; Apparatus or devices specially adapted therefor
- B01D53/84—Biological processes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/20—Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/59—Biological synthesis; Biological purification
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- General Health & Medical Sciences (AREA)
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Abstract
The present invention relates to a kind of Thiobacillus ferrooxidans Acidithiobacillus ferrooxidans ZJ 2 and its application in desulfurization；The bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center at present, and deposit number is CGMCC No.12388；Bacterial strain ZJ 2 optimum growth temperature is 28 DEG C, and the most suitable growth pH is 2.5；The strain of the present invention can be used in removing the hydrogen sulfide in all kinds of mixed gas, such as remove the hydrogen sulfide in biogas, improve the content of methane in sewage gas, the purification to biogas plays an important roll.
Description
Technical field
The invention belongs to biological treatment field, and in particular to a kind of to remove micro- life of hydrogen sulfide in all kinds of mixed gas
Thing and its application field.
Background technology
The hydrogen sulfide contained in natural gas, biogas and all kinds of foul gas generally has foul odour, hypertoxicity and strong corruption
Corrosion, is that one of main component of control is needed in the gas pollutant discharge standard of countries in the world.It is de- compared to traditional chemistry
Sulphur method, biological desulfurization can overcome the high cost of chemical method, the shortcoming of high energy consumption, with cost is low, desulfuration selectivity is high, without useless
The advantages of material is discharged, operation maintenance is simple, has become the focus of Recent study.
Because the direct oxidation effect of bacterium is not suitable for the H of higher concentration2S gas treatments, its indirectly-acting is profit
With the immobilized cell biological oxidation Fe in Biological Oxidation Tower2+, the Fe of regeneration3+Solution is in H2H is carried out in S absorption towers2S oxidation
Removing, this process can not only effectively remove the H of higher concentration2S gases, and the circulation of iron ion can be realized.
Operation principle：
Bioprocesses 4FeSO4+2H2SO4+O2→2Fe2(SO4)3+2H2O
Chemical reaction process H2S+Fe2(SO4)3→S↓+2FeSO4+H2SO4
The content of the invention
Goal of the invention：Mainly for the hydrogen sulfide contained in all kinds of mixed gas, and current minimizing technology high energy consumption,
The problem of cost is high, and a kind of microorganism that can efficiently remove hydrogen sulfide provided and low cost, efficient method, carry
For removal methods of the bacterial strain to hydrogen sulfide in all kinds of mixed gas.
Technical scheme：
A kind of Thiobacillus ferrooxidans, it is characterised in that：Classification And Nomenclature is Acidithiobacillus
FerrooxidansZJ-2, is preserved in China Microbial Culture Preservation Commission's common micro-organisms on April 21st, 2016
The heart, deposit number is CGMCC No.12388, and preservation address is the Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences
Institute of microbiology.
Bacterial strain ZJ-2 of the present invention, its 16s RNA nucleotide sequence is as shown in table 1.
Acidithiobacillus ferrooxidans ZJ-2 of the present invention screening technique is as follows：
1st, 10mL mud samples are measured first, are added in 90mL liquid 9k culture mediums, 30 DEG C, 150r/min cultures 3-
4d。
2nd, the culture medium for being enriched 3-4d is inoculated according to 10% inoculum concentration in new 100mL liquid 9k culture mediums,
30 DEG C, 150r/min cultivates 2-3d again, so repeats 3-5 times.It is enriched with during primary dcreening operation, liquid color can be by light green in bottle
It is changed into bronzing, yellow mercury oxide occurs in bottom.
3rd, the culture medium dilution spread completed will be enriched with solid 9k culture mediums, cultivated in 30 DEG C of incubators, cultivate 4d
Afterwards, advantage shaping bacterium colony is selected, cultivates and is verified whether as required bacterial strain in liquid 9k culture mediums.
Specifically, 9k culture medium prescriptions described above are as follows：
Liquid 9k culture mediums：
A liquid：(NH4)2SO43.0g, KCl 0.1g, K2HPO40.5g, Ca (NO3)20.01g, MgSO4·7H2O 0.5g,
Distilled water 800mL, pH 2.4；
B liquid：FeSO4·7H2O 22.1g, distilled water 200mL, pH 2.4；
120 DEG C of moist heat sterilization half an hour of A liquid, B liquid is put half an hour under super-clean bench ultraviolet light, mixes standby after the cooling of A liquid
With.
Solid 9k culture mediums：
A liquid：(NH4)2SO43.0g, KCl 0.1g, K2HPO40.5g, Ca (NO3)20.01g, MgSO4·7H2O 0.5g,
Distilled water 300mL, pH 2.4；
B liquid：FeSO4·7H2O 22.1g, distilled water 200mL, pH 2.4；
C liquid：Agarose (Agarose) 4g, distilled water 500mL, pH2.4；
A liquid and C liquid separate and put half an hour under moist heat sterilization, B liquid super-clean bench ultraviolet lights, are cooled to 60 DEG C, three mixes,
It is down flat plate rapidly.
The method that hydrogen sulfide in mixed gas is removed with bacterial strain ZJ-2：
1st, bacterial strain ZJ-2 activation and expansion culture：From picking single bacterium colony on 9k solid mediums, 20mL liquid is inoculated in
In 9k culture mediums, in 30 DEG C, 150r/min shaking table cultures 3 days or so, it was observed that liquid is changed into bronzing.By the seed liquor of culture
It is inoculated in by 10% inoculum concentration in fresh 100mL culture mediums, in 30 DEG C, 150r/min shaking table cultures 3 days or so, it was observed that
Obvious reddish-brown precipitation.
2nd, trickled-bed biofilter is built and biofilm：The reactor such as Fig. 2 is built, fills it up with and fills out into biological respinse tower
Material, contains bacterium culture medium according to the liquid 9k culture mediums of 10% bacterium solution+90% configuration 1L, makes culture medium in biological respinse tower inner recirculation flow
It is dynamic, temperature of reactor and air inlet amount are kept, microorganism is enriched with and is adsorbed on filler in biological respinse tower, culture medium
Using to wherein Fe2+During content deficiency original 5%, the fresh bacterium culture medium that contains is changed, is so repeated 5-6 times, until on filler
Bacterial strain can stablize consumption ferrous ion.
3rd, removings of the bacterial strain ZJ-2 to hydrogen sulfide：What into chemical reaction tower, injection biofilm was completed is rich in Fe3+Culture medium,
Culture medium is circulated between Liang Ta, hydrogen sulfide containing gas can be reached by the tower that chemically reacts and remove hydrogen sulfide
Effect.Measure air inlet and the concentration of hydrogen sulfide of gas outlet determine the removal efficiency of hydrogen sulfide respectively.
Beneficial effect：1) elemental sulfur is generated in the method course of reaction utilized in the present invention, elemental sulfur is easier from liquid phase
Middle separation, and biological sulphur is generated using upper more superior in nature；2) desulphurization cost is low, process simplification；3) to desulfurization
Selectivity is higher；4) reaction does not produce waste material, waste liquid, non-secondary pollution；5) react and efficiently carry out.
Brief description of the drawings
Table 1 is Acidithiobacillus ferrooxidans ZJ-2 16sRNA nucleotides sequence list
Fig. 1 is Acidithiobacillus ferrooxidans ZJ-2 chadogram
Fig. 2 is the structure chart of trickled-bed biofilter
Fig. 3 is the apparent figure of ZJ-2 cultures
Fig. 4 is the microbial morphology after Gram's staining
Fig. 5 is the variation diagram of hydrogen sulfide removal rate
Embodiment
Explanation and specific embodiment are described further to technical scheme below in conjunction with the accompanying drawings, but are not construed as
Limiting the scope of the invention.Experimental method used in following embodiments is conventional method without specified otherwise.
Experiment reagent consumptive material used in following embodiments etc. can be bought without specified otherwise from commercial use.
Embodiment 1
Acidithiobacillus ferrooxidans Acidithiobacillus ferrooxidans ZJ-2 separation screening；
1st, 10mL mud samples are measured first, are added in 90mL liquid 9k culture mediums, 30 DEG C, 150r/min cultures 3-
4d。
2nd, the culture medium for being enriched 3-4d is inoculated according to 10% inoculum concentration in new 100mL liquid 9k culture mediums,
30 DEG C, 150r/min cultivates 2-3d again, so repeats 3-5 times.It is enriched with during primary dcreening operation, liquid color can be by light green in bottle
It is changed into bronzing, yellow mercury oxide occurs in bottom.
3rd, the culture medium dilution spread completed will be enriched with solid 9k culture mediums, cultivated in 30 DEG C of incubators, cultivate 4d
Afterwards, advantage shaping bacterium colony is selected, cultivates and is verified whether as required bacterial strain in liquid 9k culture mediums.
Specifically, aforesaid liquid 9k culture mediums are included：A liquid：(NH4)2SO43.0g, KCl 0.1g, K2HPO40.5g, Ca
(NO3)20.01g, MgSO4·7H2O 0.5g, distilled water 800mL；B liquid：FeSO4·7H2O 22.1g, distilled water 200mL；A、B
Liquid pH uses 10mol H2SO4It is adjusted to 2.4；120 DEG C of moist heat sterilization half an hour of A liquid, it is small that B liquid puts half under super-clean bench ultraviolet light
When, mixing for standby use after the cooling of A liquid.
Solid 9k culture mediums are included：A liquid：(NH4)2SO43.0g, KCl 0.1g, K2HPO40.5g, Ca (NO3)2
0.01g, MgSO4·7H2O 0.5g, distilled water 300mL；B liquid：FeSO4·7H2O 22.1g, distilled water 200mL；C liquid：Agar
Sugar (Agarose) 4g, distilled water 500mL；The liquid of A, B, C tri- uses 10mol H2SO4It is adjusted to pH2.4；A liquid and C liquid separate damp and hot go out
Half an hour is put under bacterium, B liquid super-clean bench ultraviolet lights, 60 DEG C are cooled to, three's mixing is down flat rapidly plate.
Embodiment 2
Acidithiobacillus ferrooxidans Acidithiobacillus ferrooxidans ZJ-2 are in bioreactor
Biofilm fix；
Liquid 9k culture mediums are added, the Acidithiobacillus in logarithmic growth latter stage is accessed by 10% inoculum concentration
Ferrooxidans ZJ-2 bacterium solutions, then control to carry out under conditions of 30 DEG C of temperature, pH value 2.4, Ventilation Rate 0.4L/min
The culture of Acidithiobacillus ferrooxidans ZJ-2 bacterium.The Fe in reactor is regularly determined in incubation2+It is dense
Degree, works as Fe2+When conversion ratio is up to more than 95%, stop ventilation, reactor stands 6~8h, so that the somatic cells of growth and maturity exist
Carrier surface is substantially effectively adsorbed, and carries out the immobilization of cell.Fresh culture and renewed vaccination bacterium solution are changed afterwards, are repeated
Above-mentioned steps.Bacterium solution is not inoculated when the 5th and its afterwards replacing culture medium, under the conditions of said temperature, pH value, Ventilation Rate
Being fixed of cell for being adsorbed in carrier surface is cultivated, Fe in culture medium is during which monitored2+Change in concentration.Fe2+Conversion ratio reaches
Change culture medium when more than 95% to be cultivated again, until Fe2+Oxidation rate reaches constant, now thinks
Acidithiobacillus ferrooxidans ZJ-2 bacterium complete in the immobilization process of carrier surface, and immobilized cell enters
Enter stationary phase.Aforesaid operations, which are repeated, when changing kind of carrier carries out Acidithiobacillus ferrooxidans ZJ-2 bacterium
Immobilization.As shown in Figure 3, Figure 4.
Embodiment 3
Removal effect of the trickled-bed biofilter (as shown in Figure 2) to hydrogen sulfide in gaseous mixture；
After biofilm is stable in bioreactor, added into chemical reactor certain altitude react rich in Fe3+
Culture medium, and allow culture medium to be circulated between bioreactor and chemical reactor, it is ensured that temperature of reactor is 30 DEG C,
PH2.4, pending gas is passed through into chemical reactor, concentration of hydrogen sulfide 1200ppm, gas flow rate 5L/min determine air inlet
Mouth and the concentration of hydrogen sulfide of gas outlet, calculate the clearance of hydrogen sulfide.As shown in Figure 5.
The Acidithiobacillus ferrooxidans ZJ-2 of table 1 16sRNA nucleotides sequence lists
AGGAATCTGTCTTTTAGTGGGGGACAACCCAGGGAAACTTGGGCTAATACCGCATGAGCCCTGAGGGGG
AAAGCGGGGGATCTTCGGACCTCGCGCTAAGGGAAGAGCCTACGTCTGATTAGCTAGTTGGTAGGGTAAAGGCCTAC
CAAGGCGACGATCAGTAGCTGGTCTGAGAGGACGACCAGCCACACTGGGACTGAGACACGGCCCAGACTCCTACGGG
AGGCAGCAGTGGGGAATTTTTCGCAATGGGGGCAACCCTGACGAAGCAATGCCGCGTGGATGAAGAAGGCCTTCGGG
TTGTAAAGTCCTTTCGTGGGGGACGAAAAGGCGGGTCCTAATACGATCTGCTGTTGACGTGAACCCAAGAAGAAGCA
CCGGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGGGGGTGCAAGCGTTAATCGGAATCACTGGGCGTAAAGGGTG
CGTAGGCGGTACGTTAGGTCTGTCGTGAAATCCCCGGGCTCAACCTGGGAATGGCGGTAGAAACCGGCGCACTAGAG
TATGGGAGAGGGTGGTGGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAACATCAGTGGCGAAGGCG
GCCACCTGGCCCAATACTGACGCTGAGGCACGAAAGCGTGGGGAGCAAACAGGATTAGATACCCTGGTAGTCCACGC
CCTAAACGATGAATACTAGATGTTTGGTACCTAGCGTACTGAGTGTCGTAGCTAACGCGATAAGTATTCCGCCTGGG
AAGTACGGCCGCAAGGTTAAAACTCAAAGGAATTGACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGA
TGCAACGCGAAGAACCTTACCTGGGCTTGACATGTCCGGAATTCTGCAGAGATGCGGGAGTGCCCTTCGGGGAATCG
GAACACAGGTGCTGCATGGCTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGCGCAACCCTT
GTCCTTAGTTGCCAGCGGTTCGGCCGGGCACTCTAGGGAGACTGCCGGTGACAAACCGGAGGAAGGTGGGGATGACG
TCAAGTCCTCATGGCCTTTATGTCCAGGGCTACACACGTGCTACAATGGCGCGTACAGAGGGAAGCCAAGCCGCGAG
GTGGAGCAGACCCCAGAAAGCGCGTCGTAGTTCGGATTGCAGTCTGCAACTCGACTGCATGAAGTCGGAATCGCTAG
TAATCGCGGATCAGCATGCCGCGGTGAATACGTTCCCGGGCCTTGTACACACCGCCCG
SEQUENCE LISTING
<110> Nanjing Tech University
<120>One plant of Thiobacillus ferrooxidans and its application in desulfurization
<130> 20170427
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1282
<212> DNA
<213> Acidithiobacillus ferrooxidans ZJ-2
<400> 1
aggaatctgt cttttagtgg gggacaaccc agggaaactt gggctaatac cgcatgagcc 60
ctgaggggga aagcggggga tcttcggacc tcgcgctaag ggaagagcct acgtctgatt 120
agctagttgg tagggtaaag gcctaccaag gcgacgatca gtagctggtc tgagaggacg 180
accagccaca ctgggactga gacacggccc agactcctac gggaggcagc agtggggaat 240
ttttcgcaat gggggcaacc ctgacgaagc aatgccgcgt ggatgaagaa ggccttcggg 300
ttgtaaagtc ctttcgtggg ggacgaaaag gcgggtccta atacgatctg ctgttgacgt 360
gaacccaaga agaagcaccg gctaactccg tgccagcagc cgcggtaata cggggggtgc 420
aagcgttaat cggaatcact gggcgtaaag ggtgcgtagg cggtacgtta ggtctgtcgt 480
gaaatccccg ggctcaacct gggaatggcg gtagaaaccg gcgcactaga gtatgggaga 540
gggtggtgga attccaggtg tagcggtgaa atgcgtagag atctggagga acatcagtgg 600
cgaaggcggc cacctggccc aatactgacg ctgaggcacg aaagcgtggg gagcaaacag 660
gattagatac cctggtagtc cacgccctaa acgatgaata ctagatgttt ggtacctagc 720
gtactgagtg tcgtagctaa cgcgataagt attccgcctg ggaagtacgg ccgcaaggtt 780
aaaactcaaa ggaattgacg ggggcccgca caagcggtgg agcatgtggt ttaattcgat 840
gcaacgcgaa gaaccttacc tgggcttgac atgtccggaa ttctgcagag atgcgggagt 900
gcccttcggg gaatcggaac acaggtgctg catggctgtc gtcagctcgt gtcgtgagat 960
gttgggttaa gtcccgcaac gagcgcaacc cttgtcctta gttgccagcg gttcggccgg 1020
gcactctagg gagactgccg gtgacaaacc ggaggaaggt ggggatgacg tcaagtcctc 1080
atggccttta tgtccagggc tacacacgtg ctacaatggc gcgtacagag ggaagccaag 1140
ccgcgaggtg gagcagaccc cagaaagcgc gtcgtagttc ggattgcagt ctgcaactcg 1200
actgcatgaa gtcggaatcg ctagtaatcg cggatcagca tgccgcggtg aatacgttcc 1260
cgggccttgt acacaccgcc cg 1282
Claims (5)
1. a kind of Thiobacillus ferrooxidans, it is characterised in that：Classification And Nomenclature is Acidithiobacillus ferrooxidans
ZJ-2, is preserved in China Microbial Culture Preservation Commission's common micro-organisms center, deposit number is on April 21st, 2016
CGMCC No.12388。
2. Thiobacillus ferrooxidans according to claim 1, it is characterised in that its 16s RNA nucleotide sequence such as table 1
It is shown.
3. the screening technique of the Thiobacillus ferrooxidans described in claim 1, it is characterised in that：First with liquid 9k culture mediums
Strain is enriched with and primary dcreening operation, repeated after enrichment primary dcreening operation 3-4 time, the bacterium solution that primary dcreening operation is obtained is dilute in the progress of solid 9k culture mediums
Release and cultivate 3-4d at coating, 30 DEG C, the bacterium colony grown fine is selected from culture medium.
It is prepared by liquid 9k culture mediums：
A liquid：(NH4)2SO43.0g, KCl 0.1g, K2HPO40.5g, Ca (NO3)20.01g, MgSO4·7H2O 0.5g, distillation
Water 800mL, pH 2.4；
B liquid：FeSO4·7H2O 22.1g, distilled water 200mL, pH 2.4；
120 DEG C of moist heat sterilization half an hour of A liquid, B liquid is put half an hour under super-clean bench ultraviolet light, mixing for standby use after the cooling of A liquid；
It is prepared by solid 9k culture mediums：
A liquid：(NH4)2SO43.0g, KCl 0.1g, K2HPO40.5g, Ca (NO3)20.01g, MgSO4·7H2O 0.5g, distillation
Water 300mL, pH 2.4；
B liquid：FeSO4·7H2O 22.1g, distilled water 200mL, pH 2.4；
C liquid：Agarose (Agarose) 4g, distilled water 500mL, pH2.4；
A liquid and C liquid separate and put half an hour under moist heat sterilization, B liquid super-clean bench ultraviolet lights, are cooled to 60 DEG C, three is well mixed
It is down flat plate rapidly afterwards.
4. the application of the Thiobacillus ferrooxidans described in claim 1 or 2, it is characterised in that remove the sulphur in all kinds of gaseous mixtures
Change hydrogen.
5. the application of Thiobacillus ferrooxidans described in claim 4, it is characterised in that applying step is as follows：First, life is built
Thing drop filter reactor, filler is filled in biological respinse tower, by the bacterial strain biofilm on filler, after pending stabilised efficiency, to
The culture medium reacted is injected in chemical reaction tower, then is passed through the gas that need to be handled thereto.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108660091A (en) * | 2018-03-27 | 2018-10-16 | 常州赛尔斯生物科技有限公司 | A kind of mixed culture for removing hydrogen sulfide in H 2 S-containing gas |
| CN111265980A (en) * | 2020-01-11 | 2020-06-12 | 江苏春都环保科技有限公司 | High-efficient desulfurizing tower |
| CN114230023A (en) * | 2021-12-21 | 2022-03-25 | 常州纺织服装职业技术学院 | Method for treating sulfur-containing solid waste by microorganisms |
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| CN103184175A (en) * | 2011-12-30 | 2013-07-03 | 中国石油天然气股份有限公司 | Microbial strain used for purifying natural gas and application |
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| CN103184175A (en) * | 2011-12-30 | 2013-07-03 | 中国石油天然气股份有限公司 | Microbial strain used for purifying natural gas and application |
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| 吴莉莉等: "生物间接氧化法脱除硫化氢的研究", 《天津理工大学学报》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108660091A (en) * | 2018-03-27 | 2018-10-16 | 常州赛尔斯生物科技有限公司 | A kind of mixed culture for removing hydrogen sulfide in H 2 S-containing gas |
| CN111265980A (en) * | 2020-01-11 | 2020-06-12 | 江苏春都环保科技有限公司 | High-efficient desulfurizing tower |
| CN114230023A (en) * | 2021-12-21 | 2022-03-25 | 常州纺织服装职业技术学院 | Method for treating sulfur-containing solid waste by microorganisms |
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