CN102173878A - Method for dissolving medium/low grade phosphate ore powder by utilizing acidophilic heterotroph and acidophilic autotroph - Google Patents
Method for dissolving medium/low grade phosphate ore powder by utilizing acidophilic heterotroph and acidophilic autotroph Download PDFInfo
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Abstract
The invention relates to a method for dissolving medium/low grade phosphate ore powder by utilizing acidophilic heterotroph and acidophilic autotroph. The method is characterized by comprising the following steps: 1) activating an acidophilic heterotroph original bacterial solution to obtain an acidophilic heterotroph activated bacterial solution; 2) activating an acidophilic autotroph original bacterial solution to obtain an acidophilic autotroph activated bacterial solution; 3) mixing the acidophilic heterotroph activated bacterial solution and the acidophilic autotroph activated bacterial solution to acclimate: mixing the two bacterial solutions according to a volume ratio of 1: (0.5-1.5) to obtain a mixed bacterial solution and acclimating the mixed bacterial solution to obtain an acclimated bacterial solution; and 4) performing the biological dissolution of medium/low grade phosphate ore powder: adjusting the pH value of the culture solution D, adding the acclimated bacterial solution, and then placing the container in a 30-35 DEG C thermostatic shaker to perform shaking cultivation at 120-180rpm for 15-20 days and obtain soluble phosphorus. The method is characterized by high phosphorus-dissolving rate, simple technology and low production cost.
Description
Technical field
The present invention relates to a kind of method of utilizing acidophilic heterotrophic bacteria and having a liking for sour autotrophic bacteria dissolving mid low grade phosphate rock powder.
Background technology
China is the abundant country of a phosphate rock resource, but major part all is a mid low grade phosphate rock.In China's phosphorus ore proven reserve, the phosphorus ore average grade is about about 17%, and ore grade is no more than 10% of total reserves greater than 30% rich ore.The mid low grade phosphate rock particle is trickle, embedding cloth is tight, is to be in the status that goes out of use basically aspect the development and use of phosphate rock resource at present in China, and its reason just is that these mid low grade phosphate rock difficulties are adopted, difficult choosing, and the foreign matter content height.These factors have determined that Production Flow Chart is long when adopting conventional wet or thermal process phosphatic fertilizer production technique to handle these mid low grade phosphate rock resources, and the production cost height is low in economic efficiency.
To the requirement of phosphate fertilizer industrial expansion, Eleventh Five-Year Plan end China phosphate fertilizer production will reach 1,500 ten thousand tons, estimates that the aggregate demand of Rock Phosphate (72Min BPL) will reach 6,500 ten thousand tons according to National Development and Reform Committee.If according to present use scale, the existing rich phosphate rock resource of China will be at several years internal consumptions totally, and the more weak problem of the phosphate rock resource supportability of China is with outstanding day by day.Ministry of Land and Resources can not satisfy one of 20 mineral of national economic development requirement after phosphorus ore having been classified as 2010.Therefore, how to strengthen development and use to China's mid low grade phosphate rock resource, for the scientific utilization resource, improve the level of resources utilization, the healthy Sustainable development of keeping domestic phosphate fertilizer industry is significant, also is the problem place of this research and inquirement.
Based on reducing cost, economize on resources and day by day serious environmental issue, many investigators are in the new way of actively seeking rationally to make full use of the mid low grade phosphate rock resource, such as biological method.In recent years, utilize microorganism to cause that as inoculum dissolving mid low grade phosphate rock and the research that is translated into titanium pigment people more and more pay close attention to.From present present Research, the microorganism with molten phosphorus effect is broadly divided into two classes, promptly organic chmosynthetic heterotrophs microorganism (heterotrophic bacterium) and inorganic chemosynthetic autotroph microorganism (autotrophic bacteria).
About the molten phosphorus of heterotrophic bacterium, existing a large amount of both at home and abroad research reports has found that multiple heterotrophic bacterium all has molten phosphorus effect, and its research relates to the many aspects such as separation, seed selection, ecological distribution, molten phosphorus characteristic and plant-growth promoter action of molten phosphorus heterotrophic bacterium.Yet though obtained some good progress in the research aspect the molten phosphorus of heterotrophic bacterium at present, a problem that can not be ignored is, the organic acid acidity that molten phosphorus heterotrophic bacterium metabolism produces is not strong, and the speed of its dissolved phosphorus breeze is slow, and molten phosphorus rate is lower.
About the thought of the molten phosphorus of autotrophic bacteria, derive from the successful Application of biological in recent years leaching-out technique in the hydrometallurgy field.Biological leaching-out technique has characteristics such as Production Flow Chart is short, cost is low, environmental friendliness, and especially in the development and use of low-grade, complicated, unmanageable Mineral resources, biological leaching-out technique demonstrates powerful advantages especially.In " Chinese science frontline technology " book that the Chinese Academy of Engineering publishes, biology is leached one of the field, 21 century science and technology forward position of classifying as.The present kind of reporting of autotrophic bacteria that is used for the mid low grade phosphate rock biological dissolution is few, mainly be to have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans) and have a liking for acid oxidase sulphur thiobacillus (Acidithiobacillus thiooxidans) etc., wherein having a liking for the ferrous thiobacillus of acid oxidase is first bacterial classification that can be used in biological leaching that is separated from acidic mine water, and be considered to the leading bacterial classification in the bioleaching process, have very strong ferrous oxidation ability.Result of study before us, in the presence of pyritous, having a liking for the ferrous thiobacillus of acid oxidase can produce sulfuric acid by oxidation pyrite, thereby further dissolves mid low grade phosphate rock, leaches titanium pigment wherein.(the patent No.: ZL200510019079.3), also set forth a kind of utilization and had a liking for Thiobacillus microorganism such as the ferrous thiobacillus of acid oxidase and make phosphorus in the phosphorus ore be converted into the method for titanium pigment such as the patent of invention of authorizing in 2005 at Chi Ruan etc.Yet, although utilize the research of having a liking for sour autotrophic bacteria dissolving mid low grade phosphate rock to obtain certain progress at present, also exist bigger defective, on the low side as molten phosphorus rate, the molten phosphorus cycle is longer, molten phosphorus effect instability etc.
Summary of the invention
The object of the present invention is to provide a kind of method of utilizing acidophilic heterotrophic bacteria and having a liking for sour autotrophic bacteria dissolving mid low grade phosphate rock powder, this method has molten phosphorus rate height, technology is simple, production cost is low characteristics.
To achieve these goals, technical scheme of the present invention is: utilize acidophilic heterotrophic bacteria and have a liking for the method that sour autotrophic bacteria dissolves the mid low grade phosphate rock powder, it is characterized in that it comprises the steps:
1) activation of the original bacterium liquid of acidophilic heterotrophic bacteria:, obtain acidophilic heterotrophic bacteria activation bacterium liquid with the original bacterium liquid activation of acidophilic heterotrophic bacteria;
2) have a liking for the activation of the original bacterium liquid of sour autotrophic bacteria: will have a liking for the original bacterium liquid activation of sour autotrophic bacteria, and obtain having a liking for sour autotrophic bacteria activation bacterium liquid;
3) acidophilic heterotrophic bacteria activation bacterium liquid and have a liking for the mixing and the domestication of sour autotrophic bacteria activation bacterium liquid: acidophilic heterotrophic bacteria is activated bacterium liquid activate bacterium liquid and mix, obtain mixed bacteria liquid by 1: 0.5～1.5 volume ratio with having a liking for sour autotrophic bacteria;
Add-on (being inoculum size) by mixed bacteria liquid is 10～20% (percent by volumes) of the basic salt culture medium volume of 9K, chooses the basic salt culture medium of mixed bacteria liquid and 9K; Press proportioning=(10～15) g: 1000mL of pyritic ashes (50～200 order) and the basic salt culture medium of 9K, choose pyritic ashes; Press proportioning=(5～10) g: 1000mL of mid low grade phosphate rock powder (50～200 order) and the basic salt culture medium of 9K, choose the mid low grade phosphate rock powder; The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, is that the pH of the sulfuric acid adjusting nutrient solution C of 10～20wt% is 2.0～3.0 with concentration, adds mixed bacteria liquid again; Then container (as triangular flask) is placed 30～35 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 100～140 rev/mins of conditions, obtains taming mixed solution;
Add-on (being inoculum size) by the domestication mixed solution is 10～20% (percent by volumes) of the basic salt culture medium volume of 9K again, chooses domestication mixed solution and the basic salt culture medium of 9K; Greater than the amount of last time (carrying the content of pyritic ashes and mid low grade phosphate rock powder among the nutrient solution B gradually) at every turn, choose pyritic ashes by the proportional quantity of pyritic ashes (50～200 order) and the basic salt culture medium of 9K; Greater than the amount of last time (carrying the content of pyritic ashes and mid low grade phosphate rock powder among the nutrient solution B gradually) at every turn, choose the mid low grade phosphate rock powder by the proportional quantity of mid low grade phosphate rock powder (50～200 order) and the basic salt culture medium of 9K; The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution C is 2.0～3.0 with concentration, adds and tames mixed solution; Then container (as triangular flask) is placed 30～35 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 100～140 rev/mins of conditions, the domestication mixed solution of being tamed once more;
The inoculation domestication is 3～5 times so repeatedly, each content that improves pyritic ashes and mid low grade phosphate rock powder among the nutrient solution C gradually, in last prepared culture C: proportioning=(20～30) g: 1000mL of pyritic ashes (50～200 order) and the basic salt culture medium of 9K, proportioning=(10～20) g: 1000mL of mid low grade phosphate rock powder (50～200 order) and the basic salt culture medium of 9K; Obtain taming bacterium liquid;
4) biological dissolution of mid low grade phosphate rock powder: the add-on (being inoculum size) by domestication bacterium liquid is 10～20% (percent by volumes) of the basic salt culture medium volume of 9K, chooses domestication bacterium liquid and the basic salt culture medium of 9K (component is the same); Press proportioning=(20～30) g: 1000mL of pyritic ashes (50～200 order) and the basic salt culture medium of 9K, choose pyritic ashes; Press proportioning=(10～20) g: 1000mL of mid low grade phosphate rock powder (50～200 order) and the basic salt culture medium of 9K, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution D, is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution D is 2.0～3.0 with concentration, adds and tames bacterium liquid; Then container (as triangular flask) is placed 30～35 ℃ of constant temperature shaking tables, shaking culture is 15～20 days under 120～180 rev/mins of conditions, obtains titanium pigment.
By technique scheme, the activation of the original bacterium liquid of described acidophilic heterotrophic bacteria is: the add-on (being inoculum size) by the original bacterium liquid of acidophilic heterotrophic bacteria is 10～20% (percent by volumes) of the basic salt culture medium volume of 9K, chooses original bacterium liquid of acidophilic heterotrophic bacteria and the basic salt culture medium of 9K; The component of the basic salt culture medium of described 9K comprises: 3.0 g/L (NH
4)
2SO
4, 0.5 g/LMgSO
4.7H
2O, 0.5 g/L K
2HPO
4, 0.1 g/L KCl, 0.01 g/L Ca (NO
3)
2With the 1000mL sterilized water; Press glucose: the basic salt culture medium of 9K=(0.5～1) g: 1000mL, choose glucose;
The basic salt culture medium of 9K is packed in the container (as triangular flask), add the nutraceutical matrix of glucose as the acidophilic heterotrophic bacteria growth, obtaining nutrient solution A, is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution A is 2.0～3.0 with concentration, adds the original bacterium liquid of acidophilic heterotrophic bacteria again; Then container (as triangular flask) is placed 30～35 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 140～180 rev/mins of conditions, obtains activating bacterium liquid A;
Add-on (being inoculum size) by activation bacterium liquid A is 10～20% (percent by volumes) of the basic salt culture medium volume of 9K again, choose activation bacterium liquid A and the basic salt culture medium of 9K, press glucose: the basic salt culture medium of 9K=(0.5～1) g: 1000mL, choose glucose; The basic salt culture medium of 9K being packed in the container (as triangular flask), add the nutraceutical matrix of glucose as the acidophilic heterotrophic bacteria growth, obtain nutrient solution A, is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution A is 2.0～3.0 with concentration, adds to activate bacterium liquid A again; Then container (as triangular flask) is placed 30～35 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 140～180 rev/mins of conditions, obtains once more activatory activation bacterium liquid A; Inoculate so repeatedly 3～5 times, obtain acidophilic heterotrophic bacteria activation bacterium liquid.
By technique scheme, the described activation of having a liking for the original bacterium liquid of sour autotrophic bacteria is: be 10～20% (percent by volumes) of the basic salt culture medium volume of 9K by the add-on of having a liking for the original bacterium liquid of sour autotrophic bacteria (being inoculum size), choose and have a liking for original bacterium liquid of sour autotrophic bacteria and the basic salt culture medium of 9K (component is the same); Press FeSO
4.7H
2The proportioning of O and the basic salt culture medium of 9K=(44～45) g: 1000mL is chosen FeSO
4.7H
2O;
The basic salt culture medium of 9K is packed in the container (as triangular flask), add FeSO
4.7H
2O obtains nutrient solution B as the energy of having a liking for the growth of sour autotrophic bacteria, is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution B is 2.0～3.0 with concentration, and the original bacterium liquid of sour autotrophic bacteria is had a liking in adding again; Then container (as triangular flask) is placed 30～35 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 140～180 rev/mins of conditions, obtains activating bacterium liquid B;
Add-on (being inoculum size) by activation bacterium liquid B is 10～20% (percent by volumes) of the basic salt culture medium volume of 9K again, chooses activation bacterium liquid B and the basic salt culture medium of 9K; Press FeSO
4.7H
2The proportioning of O and the basic salt culture medium of 9K=(44～45) g: 1000mL is chosen FeSO
4.7H
2O; The basic salt culture medium of 9K is packed in the container (as triangular flask), add FeSO
4.7H
2O obtains nutrient solution B as the energy of having a liking for the growth of sour autotrophic bacteria, is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution B is 2.0～3.0 with concentration, adds to activate bacterium liquid B again; Then container (as triangular flask) is placed 30～35 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 140～180 rev/mins of conditions, obtains once more activatory activation bacterium liquid B; Inoculate so repeatedly 3～5 times, obtain having a liking for sour autotrophic bacteria activation bacterium liquid.
Described acidophilic heterotrophic bacteria and have a liking for sour autotrophic bacteria and be distributed widely in various sulfurations mineral deposit, acidic mine water and the soil can obtain by buying from the nature separation screening or from DSMZ.
Described acidophilic heterotrophic bacteria is that the acidophilic bacteria genus is had a liking for acid kind (Acidiphilium acidophilum) or hiding acidophilic bacteria (Acidiphilium cryptum), or acidophilic bacteria belongs to and to have a liking for that acid is planted and the mixed strains of hiding acidophilic bacteria, and acidophilic bacteria belongs to that to have a liking for the volume ratio that acid plants with hiding acidophilic bacteria be 1 in the mixed strains: (0.5～1.5).Their main effect is that organic meta-bolites and the residual body that produces in the sour autotrophic bacteria metabolic process had a liking in digestion, to get rid of these organic meta-bolitess and residual body to having a liking for the influence of sour autotrophic bacteria growth.
Describedly have a liking for sour autotrophic bacteria for having a liking for ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans) or iron protoxide hook end spirobacteria (Leptospirillum ferrooxidans), or have a liking for the mixed strains of ferrous thiobacillus of acid oxidase and iron protoxide hook end spirobacteria, the volume ratio of having a liking for ferrous thiobacillus of acid oxidase and iron protoxide hook end spirobacteria in the mixed strains is 1: (0.5～1.5).Having a liking for sour autotrophic bacteria can produce sulfuric acid by biological oxidation oxidation pyrite in the presence of oxidizing mediums such as pyrite, thereby dissolves the titanium pigment in the mid low grade phosphate rock powder.
P in the described mid low grade phosphate rock powder
2O
5Mass content be lower than 30%.
Described pyritic ashes and mid low grade phosphate rock powder are by the ore that market is bought or gather in the mine, and sieving by grinding makes its particle diameter reach 50～200 orders.
The invention has the beneficial effects as follows:
1, has a liking for sour autotrophic bacteria among the present invention and in process of growth, do not need to add the relatively costly organism of price as nutraceutical matrix, but with relative low price and the pyrite that obtains easily as energy derive, saved production cost, reduced energy consumption, and pyritous scientific utilization has also been played positive effect.
2, acidophilic heterotrophic bacteria can digest have a liking for organic meta-bolites and the residual body that sour autotrophic bacteria produces in molten phosphorus process among the present invention, reduce organic meta-bolites and residual body to having a liking for the toxic action of sour autotrophic bacteria, the high ferro precipitation of having a liking for sour autotrophic bacteria oxidation pyrite generation can also be reduced into ferrous state simultaneously, provide electron donor for having a liking for sour autotrophic bacteria again conversely, thereby promoted to have a liking for the dissolved efficiency of sour autotrophic bacteria centering low grade rock phosphate, the molten phosphorus rate when having a liking for sour autotrophic bacteria than independent utilization exceeds 10～20%.
3, simple, the environmental friendliness of technology.This method can be utilized the mid low grade phosphate rock resource of China's abundant comparatively fully, for the scientific utilization resource, improve the level of resources utilization, keep China's agricultural sustainable development and phosphate fertilizer industrial environment and administer and have important practical significance.
Embodiment
In order to understand the present invention better, further illustrate content of the present invention below in conjunction with embodiment, but content of the present invention not only is confined to the following examples.
Embodiment 1:
Utilize acidophilic heterotrophic bacteria and have a liking for the method that sour autotrophic bacteria dissolves the mid low grade phosphate rock powder, it comprises the steps:
1) activation of the original bacterium liquid of acidophilic heterotrophic bacteria: the add-on (being inoculum size) by the original bacterium liquid of acidophilic heterotrophic bacteria is 10% (percent by volume) of the basic salt culture medium volume of 9K, chooses original bacterium liquid of acidophilic heterotrophic bacteria and the basic salt culture medium of 9K; The component of the basic salt culture medium of described 9K comprises: 3.0 g/L (NH
4)
2SO
4, 0.5 g/L MgSO
4.7H
2O, 0.5 g/L K
2HPO
4, 0.1g/L KCl, 0.01 g/L Ca (NO
3)
2With the 1000mL sterilized water; Press glucose: the basic salt culture medium=0.5g of 9K: 1000mL, choose glucose; Described acidophilic heterotrophic bacteria is that the acidophilic bacteria genus is had a liking for acid kind (Acidiphilium acidophilum);
The basic salt culture medium of 9K being packed in the container (as triangular flask), add the nutraceutical matrix of glucose as the acidophilic heterotrophic bacteria growth, obtain nutrient solution A, is that the pH of the sulfuric acid adjusting nutrient solution A of 20wt% is 2.5 with concentration, adds the original bacterium liquid of acidophilic heterotrophic bacteria again; Then container (as triangular flask) is placed 35 ℃ of constant temperature shaking tables, shaking culture is 5 days under 160 rev/mins of conditions, obtains activating bacterium liquid A;
Add-on (being inoculum size) by activation bacterium liquid A is 10% (percent by volume) of the basic salt culture medium volume of 9K again, chooses activation bacterium liquid A and the basic salt culture medium of 9K, presses glucose: the basic salt culture medium=0.5g of 9K: 1000mL, choose glucose; The basic salt culture medium of 9K being packed in the container (as triangular flask), add the nutraceutical matrix of glucose as the acidophilic heterotrophic bacteria growth, obtain nutrient solution A, is that the pH that the sulfuric acid of 20wt% is regulated nutrient solution A is 2.5 with concentration, adds to activate bacterium liquid A again; Then container (as triangular flask) is placed 35 ℃ of constant temperature shaking tables, shaking culture is 5 days under 160 rev/mins of conditions, obtains once more activatory activation bacterium liquid A; Inoculate so repeatedly 4 times (front has been inoculated 2 times, and repeated inoculation is 2 times again), obtain acidophilic heterotrophic bacteria activation bacterium liquid;
2) have a liking for the activation of the original bacterium liquid of sour autotrophic bacteria: be 15% (percent by volume) of the basic salt culture medium volume of 9K by the add-on of having a liking for the original bacterium liquid of sour autotrophic bacteria (being inoculum size), choose and have a liking for original bacterium liquid of sour autotrophic bacteria and the basic salt culture medium of 9K (component is the same); Press FeSO
4.7H
2Proportioning=the 45g of O and the basic salt culture medium of 9K: 1000mL chooses FeSO
4.7H
2O; Describedly have a liking for sour autotrophic bacteria for having a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans);
The basic salt culture medium of 9K is packed in the container (as triangular flask), add FeSO
4.7H
2O obtains nutrient solution B as the energy of having a liking for the growth of sour autotrophic bacteria, is that the pH that the sulfuric acid of 20wt% is regulated nutrient solution B is 2.5 with concentration, and the original bacterium liquid of sour autotrophic bacteria is had a liking in adding again; Then container (as triangular flask) is placed 35 ℃ of constant temperature shaking tables, shaking culture is 4 days under 160 rev/mins of conditions, obtains activating bacterium liquid B;
Add-on (being inoculum size) by activation bacterium liquid B is 15% (percent by volume) of the basic salt culture medium volume of 9K again, chooses activation bacterium liquid B and the basic salt culture medium of 9K (component is the same); Press FeSO
4.7H
2Proportioning=the 45g of O and the basic salt culture medium of 9K: 1000mL chooses FeSO
4.7H
2O; The basic salt culture medium of 9K is packed in the container (as triangular flask), add FeSO
4.7H
2O obtains nutrient solution B as the energy of having a liking for the growth of sour autotrophic bacteria, is that the pH that the sulfuric acid of 20wt% is regulated nutrient solution B is 2.5 with concentration, adds to activate bacterium liquid B again; Then container (as triangular flask) is placed 35 ℃ of constant temperature shaking tables, shaking culture is 4 days under 160 rev/mins of conditions, obtains once more activatory activation bacterium liquid B; Inoculate so repeatedly 5 times (front has been inoculated 2 times, and repeated inoculation is 3 times again), obtain having a liking for sour autotrophic bacteria activation bacterium liquid;
3) acidophilic heterotrophic bacteria activation bacterium liquid and have a liking for the mixing and the domestication of sour autotrophic bacteria activation bacterium liquid: acidophilic heterotrophic bacteria is activated bacterium liquid activate bacterium liquid and mix, obtain mixed bacteria liquid by 1: 1 volume ratio with having a liking for sour autotrophic bacteria;
Add-on (being inoculum size) by mixed bacteria liquid is 20% (percent by volume) of the basic salt culture medium volume of 9K, chooses the basic salt culture medium of mixed bacteria liquid and 9K (component is the same); Press the proportioning=15g of pyritic ashes (100 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=8g of mid low grade phosphate rock powder (100 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, is that the pH of the sulfuric acid adjusting nutrient solution C of 20wt% is 2.5 with concentration, adds mixed bacteria liquid again; Then container (as triangular flask) is placed 35 ℃ of constant temperature shaking tables, shaking culture is 3 days under 120 rev/mins of conditions, obtains taming mixed solution;
Add-on (being inoculum size) by the domestication mixed solution is 20% (percent by volume) of the basic salt culture medium volume of 9K again, chooses domestication mixed solution and the basic salt culture medium of 9K (component is the same); Press the proportioning=20g of pyritic ashes (100 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=12g of mid low grade phosphate rock powder (100 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder; The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, is that the pH that the sulfuric acid of 20wt% is regulated nutrient solution C is 2.5 with concentration, adds and tames mixed solution; Then container (as triangular flask) is placed 35 ℃ of constant temperature shaking tables, shaking culture is 3 days under 120 rev/mins of conditions, the domestication mixed solution of being tamed once more;
5 times (domestication 2 times have been inoculated in the front in the inoculation domestication so repeatedly, the repeated inoculation domestication is 3 times again), each content that improves pyritic ashes and mid low grade phosphate rock powder among the nutrient solution C gradually, in last prepared culture C (being the 5th): the proportioning=30g of pyritic ashes (100 order) and the basic salt culture medium of 9K: 1000mL, the proportioning=20g of mid low grade phosphate rock powder (100 order) and the basic salt culture medium of 9K: 1000mL; Obtain taming bacterium liquid (the domestication bacterium liquid that adapts to mid low grade phosphate rock dissolving environment);
[annotate, in the 3rd the inoculation domestication: the proportioning=23g that presses pyritic ashes (100 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=15g of mid low grade phosphate rock powder (100 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder; In the 4th inoculation domestication: the proportioning=26g that presses pyritic ashes (100 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=18g of mid low grade phosphate rock powder (100 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder; The difference of raising amount is gradually at every turn had no particular limits];
4) biological dissolution of mid low grade phosphate rock powder: the add-on (being inoculum size) by domestication bacterium liquid is 15% (percent by volume) of the basic salt culture medium volume of 9K, chooses domestication bacterium liquid and the basic salt culture medium of 9K (component is the same); Press the proportioning=30g of pyritic ashes (100 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=20g of mid low grade phosphate rock powder (100 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution D, is that the pH that the sulfuric acid of 20wt% is regulated nutrient solution D is 2.5 with concentration, adds and tames bacterium liquid; Then container (as triangular flask) is placed 35 ℃ of constant temperature shaking tables, shaking culture is 20 days under 160 rev/mins of conditions, obtains titanium pigment.
P in the described mid low grade phosphate rock powder of present embodiment
2O
5Mass content be 18.5%, molten phosphorus rate reaches 77.8%, the molten phosphorus rate when having a liking for sour autotrophic bacteria than independent utilization exceeds 17.2%.
Embodiment 2:
Utilize acidophilic heterotrophic bacteria and have a liking for the method that sour autotrophic bacteria dissolves the mid low grade phosphate rock powder, it comprises the steps:
1) activation of the original bacterium liquid of acidophilic heterotrophic bacteria: the add-on (being inoculum size) by the original bacterium liquid of acidophilic heterotrophic bacteria is 20% (percent by volume) of the basic salt culture medium volume of 9K, chooses original bacterium liquid of acidophilic heterotrophic bacteria and the basic salt culture medium of 9K; The component of the basic salt culture medium of described 9K comprises: 3.0 g/L (NH
4)
2SO
4, 0.5 g/L MgSO
4.7H
2O, 0.5 g/L K
2HPO
4, 0.1g/L KCl, 0.01 g/L Ca (NO
3)
2With the 1000mL sterilized water; Press glucose: the basic salt culture medium=0.8g of 9K: 1000mL, choose glucose; Described acidophilic heterotrophic bacteria is for hiding acidophilic bacteria (Acidiphilium cryptum);
The basic salt culture medium of 9K being packed in the container (as triangular flask), add the nutraceutical matrix of glucose as the acidophilic heterotrophic bacteria growth, obtain nutrient solution A, is that the pH of the sulfuric acid adjusting nutrient solution A of 10wt% is 2.8 with concentration, adds the original bacterium liquid of acidophilic heterotrophic bacteria again; Then container (as triangular flask) is placed 30 ℃ of constant temperature shaking tables, shaking culture is 4 days under 140 rev/mins of conditions, obtains activating bacterium liquid A;
Add-on (being inoculum size) by activation bacterium liquid A is 20% (percent by volume) of the basic salt culture medium volume of 9K again, chooses activation bacterium liquid A and the basic salt culture medium of 9K, presses glucose: the basic salt culture medium=0.8g of 9K: 1000mL, choose glucose; The basic salt culture medium of 9K being packed in the container (as triangular flask), add the nutraceutical matrix of glucose as the acidophilic heterotrophic bacteria growth, obtain nutrient solution A, is that the pH that the sulfuric acid of 10wt% is regulated nutrient solution A is 2.8 with concentration, adds to activate bacterium liquid A again; Container (as triangular flask) is placed 30 ℃ of constant temperature shaking tables, and shaking culture is 4 days under 140 rev/mins of conditions, obtains once more activatory activation bacterium liquid A; Inoculate so repeatedly 5 times (front has been inoculated 2 times, and repeated inoculation is 3 times again), obtain acidophilic heterotrophic bacteria activation bacterium liquid;
2) have a liking for the activation of the original bacterium liquid of sour autotrophic bacteria: be 10% (percent by volume) of the basic salt culture medium volume of 9K by the add-on of having a liking for the original bacterium liquid of sour autotrophic bacteria (being inoculum size), choose and have a liking for original bacterium liquid of sour autotrophic bacteria and the basic salt culture medium of 9K (component is the same); Press FeSO
4.7H
2Proportioning=the 44g of O and the basic salt culture medium of 9K: 1000mL chooses FeSO
4.7H
2O; It is described that to have a liking for sour autotrophic bacteria be iron protoxide hook end spirobacteria (Leptospirillum ferrooxidans);
The basic salt culture medium of 9K is packed in the container (as triangular flask), add FeSO
4.7H
2O obtains nutrient solution B as the energy of having a liking for the growth of sour autotrophic bacteria, is that the pH that the sulfuric acid of 10wt% is regulated nutrient solution B is 2.8 with concentration, and the original bacterium liquid of sour autotrophic bacteria is had a liking in adding again; Then container (as triangular flask) is placed 30 ℃ of constant temperature shaking tables, shaking culture is 5 days under 140 rev/mins of conditions, obtains activating bacterium liquid B;
Add-on (being inoculum size) by activation bacterium liquid B is 10% (percent by volume) of the basic salt culture medium volume of 9K again, chooses activation bacterium liquid B and the basic salt culture medium of 9K (component is the same); Press FeSO
4.7H
20 with the proportioning=44g of the basic salt culture medium of 9K: 1000mL, choose FeSO
4.7H
2O; The basic salt culture medium of 9K is packed in the container (as triangular flask), add FeSO
4.7H
2O obtains nutrient solution B as the energy of having a liking for the growth of sour autotrophic bacteria, is that the pH that the sulfuric acid of 10wt% is regulated nutrient solution B is 2.0～3.0 with concentration, adds to activate bacterium liquid B again; Container (as triangular flask) is placed 30 ℃ of constant temperature shaking tables, and shaking culture is 5 days under 140 rev/mins of conditions, obtains once more activatory activation bacterium liquid B; Inoculate so repeatedly 4 times (front has been inoculated 2 times, and repeated inoculation is 4 times again), obtain having a liking for sour autotrophic bacteria activation bacterium liquid;
3) acidophilic heterotrophic bacteria activation bacterium liquid and have a liking for the mixing and the domestication of sour autotrophic bacteria activation bacterium liquid: acidophilic heterotrophic bacteria is activated bacterium liquid activate bacterium liquid and mix, obtain mixed bacteria liquid by 1: 0.8 volume ratio with having a liking for sour autotrophic bacteria;
Add-on (being inoculum size) by mixed bacteria liquid is 15% (percent by volume) of the basic salt culture medium volume of 9K, chooses the basic salt culture medium of mixed bacteria liquid and 9K (component is the same); Press the proportioning=12g of pyritic ashes (50 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=10g of mid low grade phosphate rock powder (100 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, is that the pH of the sulfuric acid adjusting nutrient solution C of 10wt% is 2.8 with concentration, adds mixed bacteria liquid again; Then container (as triangular flask) is placed 30 ℃ of constant temperature shaking tables, shaking culture is 4 days under 140 rev/mins of conditions, obtains taming mixed solution;
Add-on (being inoculum size) by the domestication mixed solution is 15% (percent by volume) of the basic salt culture medium volume of 9K again, chooses domestication mixed solution and the basic salt culture medium of 9K (component is the same); Press the proportioning=15g of pyritic ashes (50 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=12g of mid low grade phosphate rock powder (100 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder; The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, is that the pH that the sulfuric acid of 10wt% is regulated nutrient solution C is 2.8 with concentration, adds and tames mixed solution; Then container (as triangular flask) is placed 30 ℃ of constant temperature shaking tables, shaking culture is 4 days under 140 rev/mins of conditions, the domestication mixed solution of being tamed once more;
4 times (domestication 2 times have been inoculated in the front in the inoculation domestication so repeatedly, the repeated inoculation domestication is 2 times again), each content that improves pyritic ashes and mid low grade phosphate rock powder among the nutrient solution C gradually, in last prepared culture C (being the 4th): the proportioning=25g of pyritic ashes (50 order) and the basic salt culture medium of 9K: 1000mL, the proportioning=18g of mid low grade phosphate rock powder (100 order) and the basic salt culture medium of 9K: 1000mL; Obtain taming bacterium liquid (the domestication bacterium liquid that adapts to mid low grade phosphate rock dissolving environment); [annotate, in the 3rd the inoculation domestication: the proportioning=20g that presses pyritic ashes (50 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=15g of mid low grade phosphate rock powder (100 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder; Raising amount is gradually at every turn had no particular limits]
4) biological dissolution of mid low grade phosphate rock powder: the add-on (being inoculum size) by domestication bacterium liquid is 18% (percent by volume) of the basic salt culture medium volume of 9K, chooses domestication bacterium liquid and the basic salt culture medium of 9K (component is the same); Press the proportioning=25g of pyritic ashes (50 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=18g of mid low grade phosphate rock powder (100 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution D, is that the pH that the sulfuric acid of 10wt% is regulated nutrient solution D is 2.8 with concentration, adds and tames bacterium liquid; Then container (as triangular flask) is placed 30 ℃ of constant temperature shaking tables, shaking culture is 18 days under 140 rev/mins of conditions, obtains titanium pigment.
P in the described mid low grade phosphate rock powder of present embodiment
2O
5Mass content be 25%, molten phosphorus rate reaches 72.5%, the molten phosphorus rate when having a liking for sour autotrophic bacteria than independent utilization exceeds 12.4%.
Embodiment 3:
Utilize acidophilic heterotrophic bacteria and have a liking for the method that sour autotrophic bacteria dissolves the mid low grade phosphate rock powder, it comprises the steps:
1) activation of the original bacterium liquid of acidophilic heterotrophic bacteria: the add-on (being inoculum size) by the original bacterium liquid of acidophilic heterotrophic bacteria is 20% (percent by volume) of the basic salt culture medium volume of 9K, chooses original bacterium liquid of acidophilic heterotrophic bacteria and the basic salt culture medium of 9K; Press glucose: the basic salt culture medium=1g of 9K: 1000mL, choose glucose;
The component of the basic salt culture medium of described 9K comprises: 3.0 g/L (NH
4)
2SO
4, 0.5 g/L MgSO
4.7H
2O, 0.5 g/L K
2HPO
4, 0.1 g/L KCl, 0.01 g/L Ca (NO
3)
2With the 1000mL sterilized water; Described acidophilic heterotrophic bacteria is that acidophilic bacteria belongs to and to have a liking for the mixed strains that (Acidiphilium acidophilum) and hiding acidophilic bacteria (Acidiphilium cryptum) are planted in acid, and acidophilic bacteria belongs to and has a liking for that acid is planted and the volume ratio of hiding acidophilic bacteria is 1: 1 in the mixed strains;
The basic salt culture medium of 9K being packed in the container (as triangular flask), add the nutraceutical matrix of glucose as the acidophilic heterotrophic bacteria growth, obtain nutrient solution A, is that the pH of the sulfuric acid adjusting nutrient solution A of 15wt% is 3.0 with concentration, adds the original bacterium liquid of acidophilic heterotrophic bacteria again; Then container (as triangular flask) is placed 32 ℃ of constant temperature shaking tables, shaking culture is 3 days under 180 rev/mins of conditions, obtains activating bacterium liquid A;
Add-on (being inoculum size) by activation bacterium liquid A is 20% (percent by volume) of the basic salt culture medium volume of 9K again, chooses activation bacterium liquid A and the basic salt culture medium of 9K, presses glucose: the basic salt culture medium=1g of 9K: 1000mL, choose glucose; The basic salt culture medium of 9K being packed in the container (as triangular flask), add the nutraceutical matrix of glucose as the acidophilic heterotrophic bacteria growth, obtain nutrient solution A, is that the pH that the sulfuric acid of 15wt% is regulated nutrient solution A is 3.0 with concentration, adds to activate bacterium liquid A again; Then container (as triangular flask) is placed 32 ℃ of constant temperature shaking tables, shaking culture is 3 days under 180 rev/mins of conditions, obtains once more activatory activation bacterium liquid A; Inoculate so repeatedly 4 times (front has been inoculated 2 times, and repeated inoculation is 2 times again), obtain acidophilic heterotrophic bacteria activation bacterium liquid;
2) have a liking for the activation of the original bacterium liquid of sour autotrophic bacteria: be 20% (percent by volume) of the basic salt culture medium volume of 9K by the add-on of having a liking for the original bacterium liquid of sour autotrophic bacteria (being inoculum size), choose and have a liking for original bacterium liquid of sour autotrophic bacteria and the basic salt culture medium of 9K (component is the same); Press FeSO
4.7H
2Proportioning=the 44g of O and the basic salt culture medium of 9K: 1000mL chooses FeSO
4.7H
2O;
Describedly have a liking for sour autotrophic bacteria for to have a liking for the mixed strains of ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans) and iron protoxide hook end spirobacteria (Leptospirillum ferrooxidans), the volume ratio of having a liking for ferrous thiobacillus of acid oxidase and iron protoxide hook end spirobacteria in the mixed strains is 1: 1;
The basic salt culture medium of 9K is packed in the container (as triangular flask), add FeSO
4.7H
2O obtains nutrient solution B as the energy of having a liking for the growth of sour autotrophic bacteria, is that the pH that the sulfuric acid of 15wt% is regulated nutrient solution B is 3.0 with concentration, and the original bacterium liquid of sour autotrophic bacteria is had a liking in adding again; Then container (as triangular flask) is placed 32 ℃ of constant temperature shaking tables, shaking culture is 4 days under 180 rev/mins of conditions, obtains activating bacterium liquid B;
Add-on (being inoculum size) by activation bacterium liquid B is 20% (percent by volume) of the basic salt culture medium volume of 9K again, chooses activation bacterium liquid B and the basic salt culture medium of 9K (component is the same); Press FeSO
4.7H
2Proportioning=the 44g of O and the basic salt culture medium of 9K: 1000mL chooses FeSO
4.7H
2O; The basic salt culture medium of 9K is packed in the container (as triangular flask), add FeSO
4.7H
2O obtains nutrient solution B as the energy of having a liking for the growth of sour autotrophic bacteria, is that the pH that the sulfuric acid of 15wt% is regulated nutrient solution B is 3.0 with concentration, adds to activate bacterium liquid B again; Then container (as triangular flask) is placed 32 ℃ of constant temperature shaking tables, shaking culture is 4 days under 180 rev/mins of conditions, obtains once more activatory activation bacterium liquid B; Inoculate so repeatedly 3 times (front has been inoculated 2 times, and repeated inoculation is 1 time again), obtain having a liking for sour autotrophic bacteria activation bacterium liquid;
3) acidophilic heterotrophic bacteria activation bacterium liquid and have a liking for the mixing and the domestication of sour autotrophic bacteria activation bacterium liquid: acidophilic heterotrophic bacteria is activated bacterium liquid activate bacterium liquid and mix, obtain mixed bacteria liquid by 1: 1 volume ratio with having a liking for sour autotrophic bacteria;
Add-on (being inoculum size) by mixed bacteria liquid is 20% (percent by volume) of the basic salt culture medium volume of 9K, chooses the basic salt culture medium of mixed bacteria liquid and 9K (component is the same); Press the proportioning=10g of pyritic ashes (200 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=7g of mid low grade phosphate rock powder (50 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, is that the pH of the sulfuric acid adjusting nutrient solution C of 15wt% is 3.0 with concentration, adds mixed bacteria liquid again; Container (as triangular flask) is placed 32 ℃ of constant temperature shaking tables, and shaking culture is 4 days under 100 rev/mins of conditions, obtains taming mixed solution;
The 2nd inoculation domestication: the add-on (being inoculum size) by the domestication mixed solution is 20% (percent by volume) of the basic salt culture medium volume of 9K again, chooses domestication mixed solution and the basic salt culture medium of 9K (component is the same); Press the proportioning=15g of pyritic ashes (200 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=10g of mid low grade phosphate rock powder (50 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder; The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C2, is that the pH that the sulfuric acid of 15wt% is regulated nutrient solution C2 is 3.0 with concentration, adds and tames mixed solution; Container (as triangular flask) is placed 32 ℃ of constant temperature shaking tables, and shaking culture is 4 days under 100 rev/mins of conditions, the domestication mixed solution of being tamed once more;
The 3rd inoculation domestication: the add-on (being inoculum size) by the domestication mixed solution of taming once more is 20% (percent by volume) of the basic salt culture medium volume of 9K once more, chooses the domestication mixed solution and the basic salt culture medium of 9K (component is the same) of domestication once more; Press the proportioning=20g of pyritic ashes (200 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=15g of mid low grade phosphate rock powder (50 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder; The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C3, is that the pH that the sulfuric acid of 15wt% is regulated nutrient solution C3 is 3.0 with concentration, adds the domestication mixed solution of taming once more again; Then container (as triangular flask) is placed 32 ℃ of constant temperature shaking tables, shaking culture is 4 days under 100 rev/mins of conditions, obtains taming bacterium liquid;
[annotate, inoculation has been tamed 3 times repeatedly, each content that improves pyritic ashes and mid low grade phosphate rock powder in each prepared culture gradually, in last prepared culture (promptly the 3rd time): the proportioning=20g of pyritic ashes (200 order) and the basic salt culture medium of 9K: 1000mL, the proportioning=15g of mid low grade phosphate rock powder (50 order) and the basic salt culture medium of 9K: 1000mL];
4) biological dissolution of mid low grade phosphate rock powder: the add-on (being inoculum size) by domestication bacterium liquid is 12% (percent by volume) of the basic salt culture medium volume of 9K, chooses domestication bacterium liquid and the basic salt culture medium of 9K (component is the same); Press the proportioning=20g of pyritic ashes (200 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=15g of mid low grade phosphate rock powder (50 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution D, is that the pH that the sulfuric acid of 15wt% is regulated nutrient solution D is 3.0 with concentration, adds and tames bacterium liquid; Then container (as triangular flask) is placed 32 ℃ of constant temperature shaking tables, shaking culture is 15 days under 180 rev/mins of conditions, obtains titanium pigment.
P in the described mid low grade phosphate rock powder of present embodiment
2O
5Mass content be 26.3%, molten phosphorus rate reaches 63.6%, the molten phosphorus rate when having a liking for sour autotrophic bacteria than independent utilization exceeds 15.7%.
Embodiment 4:
Utilize acidophilic heterotrophic bacteria and have a liking for the method that sour autotrophic bacteria dissolves the mid low grade phosphate rock powder, it comprises the steps:
1) activation of the original bacterium liquid of acidophilic heterotrophic bacteria:, obtain acidophilic heterotrophic bacteria activation bacterium liquid with the original bacterium liquid activation of acidophilic heterotrophic bacteria; Described acidophilic heterotrophic bacteria is that the acidophilic bacteria genus is had a liking for acid kind (Acidiphilium acidophilum);
2) have a liking for the activation of the original bacterium liquid of sour autotrophic bacteria: will have a liking for the original bacterium liquid activation of sour autotrophic bacteria, and obtain having a liking for sour autotrophic bacteria activation bacterium liquid; Describedly have a liking for sour autotrophic bacteria for having a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans);
3) acidophilic heterotrophic bacteria activation bacterium liquid and have a liking for the mixing and the domestication of sour autotrophic bacteria activation bacterium liquid: acidophilic heterotrophic bacteria is activated bacterium liquid activate bacterium liquid and mix, obtain mixed bacteria liquid by 1: 0.5 volume ratio with having a liking for sour autotrophic bacteria;
Add-on (being inoculum size) by mixed bacteria liquid is 10% (percent by volume) of the basic salt culture medium volume of 9K, chooses the basic salt culture medium of mixed bacteria liquid and 9K; Press the proportioning=10g of pyritic ashes (50 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=5g of mid low grade phosphate rock powder (50 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, is that the pH of the sulfuric acid adjusting nutrient solution C of 10wt% is 2.0 with concentration, adds mixed bacteria liquid again; Then container (as triangular flask) is placed 30 ℃ of constant temperature shaking tables, shaking culture is 3 days under 100 rev/mins of conditions, obtains taming mixed solution;
The inoculation domestication is 3 times so repeatedly, each content that improves pyritic ashes and mid low grade phosphate rock powder among the nutrient solution C gradually, in last prepared culture C: the proportioning=20g of pyritic ashes (50 order) and the basic salt culture medium of 9K: 1000mL, the proportioning=10g of mid low grade phosphate rock powder (50 order) and the basic salt culture medium of 9K: 1000mL; Obtain taming bacterium liquid;
4) biological dissolution of mid low grade phosphate rock powder: the add-on (being inoculum size) by domestication bacterium liquid is 10% (percent by volume) of the basic salt culture medium volume of 9K, chooses domestication bacterium liquid and the basic salt culture medium of 9K (component is the same); Press the proportioning=20g of pyritic ashes (50 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=10g of mid low grade phosphate rock powder (50 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution D, is that the pH that the sulfuric acid of 10wt% is regulated nutrient solution D is 2.0 with concentration, adds and tames bacterium liquid; Then container (as triangular flask) is placed 30 ℃ of constant temperature shaking tables, shaking culture is 15 days under 120 rev/mins of conditions, obtains titanium pigment.
P in the described mid low grade phosphate rock powder of present embodiment
2O
5Mass content be 27.8%, molten phosphorus rate reaches 72.6%, the molten phosphorus rate when having a liking for sour autotrophic bacteria than independent utilization exceeds 13.7%.
Embodiment 5:
Utilize acidophilic heterotrophic bacteria and have a liking for the method that sour autotrophic bacteria dissolves the mid low grade phosphate rock powder, it comprises the steps:
1) activation of the original bacterium liquid of acidophilic heterotrophic bacteria:, obtain acidophilic heterotrophic bacteria activation bacterium liquid with the original bacterium liquid activation of acidophilic heterotrophic bacteria; Described acidophilic heterotrophic bacteria is for hiding acidophilic bacteria (Acidiphilium cryptum);
2) have a liking for the activation of the original bacterium liquid of sour autotrophic bacteria: will have a liking for the original bacterium liquid activation of sour autotrophic bacteria, and obtain having a liking for sour autotrophic bacteria activation bacterium liquid; It is described that to have a liking for sour autotrophic bacteria be iron protoxide hook end spirobacteria (Leptospirillum ferrooxidans);
3) acidophilic heterotrophic bacteria activation bacterium liquid and have a liking for the mixing and the domestication of sour autotrophic bacteria activation bacterium liquid: acidophilic heterotrophic bacteria is activated bacterium liquid activate bacterium liquid and mix, obtain mixed bacteria liquid by 1: 1.5 volume ratio with having a liking for sour autotrophic bacteria;
Add-on (being inoculum size) by mixed bacteria liquid is 20% (percent by volume) of the basic salt culture medium volume of 9K, chooses the basic salt culture medium of mixed bacteria liquid and 9K; Press the proportioning=15g of pyritic ashes (200 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=10g of mid low grade phosphate rock powder (200 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, is that the pH of the sulfuric acid adjusting nutrient solution C of 20wt% is 3.0 with concentration, adds mixed bacteria liquid again; Container (as triangular flask) is placed 34 ℃ of constant temperature shaking tables, and shaking culture is 5 days under 140 rev/mins of conditions, obtains taming mixed solution;
The inoculation domestication is 5 times so repeatedly, each content that improves pyritic ashes and mid low grade phosphate rock powder among the nutrient solution C gradually, in last prepared culture C: the proportioning=30g of pyritic ashes (200 order) and the basic salt culture medium of 9K: 1000mL, the proportioning=20g of mid low grade phosphate rock powder (200 order) and the basic salt culture medium of 9K: 1000mL; Obtain taming bacterium liquid;
4) biological dissolution of mid low grade phosphate rock powder: the add-on (being inoculum size) by domestication bacterium liquid is 20% (percent by volume) of the basic salt culture medium volume of 9K, chooses domestication bacterium liquid and the basic salt culture medium of 9K (component is the same); Press the proportioning=30g of pyritic ashes (200 order) and the basic salt culture medium of 9K: 1000mL, choose pyritic ashes; Press the proportioning=20g of mid low grade phosphate rock powder (200 order) and the basic salt culture medium of 9K: 1000mL, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container (as triangular flask), add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution D, is that the pH that the sulfuric acid of 20wt% is regulated nutrient solution D is 3.0 with concentration, adds and tames bacterium liquid; Then container (as triangular flask) is placed 34 ℃ of constant temperature shaking tables, shaking culture is 20 days under 180 rev/mins of conditions, obtains titanium pigment.
P in the described mid low grade phosphate rock powder of present embodiment
2O
5Mass content be 22.7%, molten phosphorus rate reaches 78.5%, the molten phosphorus rate when having a liking for sour autotrophic bacteria than independent utilization exceeds 18.7%.
The bound of each raw material that the present invention is cited, interval value, and the bound of processing parameter (as temperature, time etc.), interval value can both realize the present invention, do not enumerate embodiment one by one at this.
Claims (8)
1. utilize acidophilic heterotrophic bacteria and have a liking for the method that sour autotrophic bacteria dissolves the mid low grade phosphate rock powder, it is characterized in that it comprises the steps:
1) activation of the original bacterium liquid of acidophilic heterotrophic bacteria:, obtain acidophilic heterotrophic bacteria activation bacterium liquid with the original bacterium liquid activation of acidophilic heterotrophic bacteria;
2) have a liking for the activation of the original bacterium liquid of sour autotrophic bacteria: will have a liking for the original bacterium liquid activation of sour autotrophic bacteria, and obtain having a liking for sour autotrophic bacteria activation bacterium liquid;
3) acidophilic heterotrophic bacteria activation bacterium liquid and have a liking for mixing, the domestication of sour autotrophic bacteria activation bacterium liquid: acidophilic heterotrophic bacteria is activated bacterium liquid activate bacterium liquid and mix, obtain mixed bacteria liquid by 1: 0.5～1.5 volume ratio with having a liking for sour autotrophic bacteria;
Add-on by mixed bacteria liquid is 10～20% of the basic salt culture medium volume of 9K, chooses the basic salt culture medium of mixed bacteria liquid and 9K; Press proportioning=(10～15) g: 1000mL of pyritic ashes and the basic salt culture medium of 9K, choose pyritic ashes; Press proportioning=(5～10) g: 1000mL of mid low grade phosphate rock powder and the basic salt culture medium of 9K, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution C, is that the pH of the sulfuric acid adjusting nutrient solution C of 10～20wt% is 2.0～3.0 with concentration, adds mixed bacteria liquid again; Then container is placed 30～35 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 100～140 rev/mins of conditions, obtains taming mixed solution;
The inoculation domestication is 3～5 times so repeatedly, each content that improves pyritic ashes and mid low grade phosphate rock powder among the nutrient solution C gradually, in last prepared culture C: the proportioning of pyritic ashes and the basic salt culture medium of 9K=(20～30) g: 1000mL, the proportioning of mid low grade phosphate rock powder and the basic salt culture medium of 9K=(10～20) g: 1000mL; Obtain taming bacterium liquid;
4) biological dissolution of mid low grade phosphate rock powder: the add-on by domestication bacterium liquid is 10～20% of the basic salt culture medium volume of 9K, chooses domestication bacterium liquid and the basic salt culture medium of 9K; Press proportioning=(20～30) g: 1000mL of pyritic ashes and the basic salt culture medium of 9K, choose pyritic ashes; Press proportioning=(10～20) g: 1000mL of mid low grade phosphate rock powder and the basic salt culture medium of 9K, choose the mid low grade phosphate rock powder;
The basic salt culture medium of 9K being packed in the container, add pyritic ashes and mid low grade phosphate rock powder, obtain nutrient solution D, is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution D is 2.0～3.0 with concentration, adds and tames bacterium liquid; Then container is placed 30～35 ℃ of constant temperature shaking tables, shaking culture is 15～20 days under 120～180 rev/mins of conditions, obtains titanium pigment.
2. the method for utilizing acidophilic heterotrophic bacteria and having a liking for sour autotrophic bacteria dissolving mid low grade phosphate rock powder according to claim 1, it is characterized in that: the activation of the original bacterium liquid of described acidophilic heterotrophic bacteria is: the add-on by the original bacterium liquid of acidophilic heterotrophic bacteria is 10～20% of the basic salt culture medium volume of 9K, chooses original bacterium liquid of acidophilic heterotrophic bacteria and the basic salt culture medium of 9K; Press glucose: the basic salt culture medium of 9K=(0.5～1) g: 1000mL, choose glucose; The basic salt culture medium of 9K is packed in the container, add glucose, obtain nutrient solution A, with concentration is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution A is 2.0～3.0, add the original bacterium liquid of acidophilic heterotrophic bacteria again, container is placed 30～35 ℃ of constant temperature shaking tables, and shaking culture is 3～5 days under 140～180 rev/mins of conditions, obtains activating bacterium liquid A;
Add-on by activation bacterium liquid A is 10～20% of the basic salt culture medium volume of 9K again, chooses activation bacterium liquid A and the basic salt culture medium of 9K, presses glucose: the basic salt culture medium of 9K=(0.5～1) g: 1000mL, choose glucose; The basic salt culture medium of 9K being packed in the container, add glucose, obtain nutrient solution A, is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution A is 2.0～3.0 with concentration, adds to activate bacterium liquid A again; Then container is placed 30～35 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 140～180 rev/mins of conditions, obtains once more activatory activation bacterium liquid A; Inoculate so repeatedly 3～5 times, obtain acidophilic heterotrophic bacteria activation bacterium liquid.
3. the method for utilizing acidophilic heterotrophic bacteria and having a liking for sour autotrophic bacteria dissolving mid low grade phosphate rock powder according to claim 1, it is characterized in that: the described activation of having a liking for the original bacterium liquid of sour autotrophic bacteria is: by the add-on of having a liking for the original bacterium liquid of sour autotrophic bacteria is 10～20% of the basic salt culture medium volume of 9K, chooses and has a liking for original bacterium liquid of sour autotrophic bacteria and the basic salt culture medium of 9K; Press FeSO
4.7H
2The proportioning of O and the basic salt culture medium of 9K=(44～45) g: 1000mL is chosen FeSO
4.7H
2O; The basic salt culture medium of 9K is packed in the container, add FeSO
4.7H
2O obtains nutrient solution B, is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution B is 2.0～3.0 with concentration, adds and has a liking for the original bacterium liquid of sour autotrophic bacteria; Then container is placed 30～35 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 140～180 rev/mins of conditions, obtains activating bacterium liquid B;
Add-on by activation bacterium liquid B is 10～20% of the basic salt culture medium volume of 9K again, chooses activation bacterium liquid B and the basic salt culture medium of 9K; Press FeSO
4.7H
2The proportioning of O and the basic salt culture medium of 9K=(44～45) g: 1000mL is chosen FeSO
4.7H
2O; The basic salt culture medium of 9K is packed in the container, add FeSO
4.7H
2O obtains nutrient solution B, is that the pH that the sulfuric acid of 10～20wt% is regulated nutrient solution B is 2.0～3.0 with concentration, adds activation bacterium liquid B again; Then container is placed 30～35 ℃ of constant temperature shaking tables, shaking culture is 3～5 days under 140～180 rev/mins of conditions, obtains once more activatory activation bacterium liquid B; Inoculate so repeatedly 3～5 times, obtain having a liking for sour autotrophic bacteria activation bacterium liquid.
4. according to claim 1 or the 2 or 3 described methods of utilizing acidophilic heterotrophic bacteria and having a liking for sour autotrophic bacteria dissolving mid low grade phosphate rock powder, it is characterized in that: the component of the basic salt culture medium of described 9K comprises: 3.0 g/L (NH
4)
2SO
4, 0.5 g/L MgSO
4.7H
2O, 0.5 g/L K
2HPO
4, 0.1 g/L KCl, 0.01 g/L Ca (NO
3)
2With the 1000mL sterilized water.
5. the method for utilizing acidophilic heterotrophic bacteria and having a liking for sour autotrophic bacteria dissolving mid low grade phosphate rock powder according to claim 1 and 2, it is characterized in that: described acidophilic heterotrophic bacteria is that the acidophilic bacteria genus is had a liking for acid kind (Acidiphilium acidophilum) or hiding acidophilic bacteria (Acidiphilium cryptum), or acidophilic bacteria belongs to and to have a liking for that acid is planted and the mixed strains of hiding acidophilic bacteria, and acidophilic bacteria belongs to that to have a liking for the volume ratio that acid plants with hiding acidophilic bacteria be 1 in the mixed strains: (0.5～1.5).
6. according to claim 1 or the 3 described methods of utilizing acidophilic heterotrophic bacteria and having a liking for sour autotrophic bacteria dissolving mid low grade phosphate rock powder, it is characterized in that: describedly have a liking for sour autotrophic bacteria for having a liking for ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans) or iron protoxide hook end spirobacteria (Leptospirillum ferrooxidans), or have a liking for the mixed strains of ferrous thiobacillus of acid oxidase and iron protoxide hook end spirobacteria, the volume ratio of having a liking for ferrous thiobacillus of acid oxidase and iron protoxide hook end spirobacteria in the mixed strains is 1: (0.5～1.5).
7. the method for utilizing acidophilic heterotrophic bacteria and having a liking for sour autotrophic bacteria dissolving mid low grade phosphate rock powder according to claim 1 is characterized in that: the P in the described mid low grade phosphate rock powder
2O
5Mass content be lower than 30%, the particle diameter of mid low grade phosphate rock powder is 50～200 orders.
8. the method for utilizing acidophilic heterotrophic bacteria and having a liking for sour autotrophic bacteria dissolving mid low grade phosphate rock powder according to claim 1, it is characterized in that: the particle diameter of described pyritic ashes is 50～200 orders.
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| CN109205801A (en) * | 2018-11-16 | 2019-01-15 | 中南大学 | Utilize autotrophic type-heterotrophic bacterium collaboration processing waste water containing trivalent arsenic method |
| CN109321558A (en) * | 2018-10-31 | 2019-02-12 | 武汉工程大学 | The method for preparing phosphate solubilizing microorganism sustained release sodium alginate micro ball using emulsion process |
| CN111733324A (en) * | 2020-07-24 | 2020-10-02 | 武汉工程大学 | Method for removing phosphorus in high-phosphorus iron ore by using acidophilic heterotrophic bacteria and acidophilic autotrophic bacteria |
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Cited By (5)
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| CN109321558A (en) * | 2018-10-31 | 2019-02-12 | 武汉工程大学 | The method for preparing phosphate solubilizing microorganism sustained release sodium alginate micro ball using emulsion process |
| CN109321558B (en) * | 2018-10-31 | 2022-12-06 | 武汉工程大学 | Method for preparing phosphate-solubilizing microorganism slow-release sodium alginate microspheres by using emulsification method |
| CN109205801A (en) * | 2018-11-16 | 2019-01-15 | 中南大学 | Utilize autotrophic type-heterotrophic bacterium collaboration processing waste water containing trivalent arsenic method |
| CN111733324A (en) * | 2020-07-24 | 2020-10-02 | 武汉工程大学 | Method for removing phosphorus in high-phosphorus iron ore by using acidophilic heterotrophic bacteria and acidophilic autotrophic bacteria |
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