CN102329957B - Method for continuously leaching sulfide ore by using synergy of autotrophic ore leaching bacteria and heterotrophic ore leaching bacteria - Google Patents
Method for continuously leaching sulfide ore by using synergy of autotrophic ore leaching bacteria and heterotrophic ore leaching bacteria Download PDFInfo
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Abstract
The invention discloses a method for continuously leaching sulfide ore by using the synergy of autotrophic ore leaching bacteria and heterotrophic ore leaching bacteria, which comprises two main steps of preparing a compound ore leaching strain and leaching ore by using the compound strain. The step of preparing the compound ore leaching strain comprises the steps of selecting a strain, preparing a culture medium, compositely culturing the strain and domesticating the compound ore leaching strain. The step of leaching ore by using the compound strain comprises the steps of pretreating an ore sample, preparing an ore leaching culture medium, leaching the ore by using the compound ore leaching strain, selecting and adding semiconductor sulfide ore and extracting metals in the leaching liquids. In the method, the characteristic of the semiconductor sulfide ore to provide electrons to promote the ore leaching action of microbes in the electron transition process is utilized, so that the operating cost of the whole process is lowered, the ore leaching efficiency of the process is improved, and the method has broad application prospect in the field of metallurgy.
Description
Technical field
The present invention relates to the method utilizing autotrophic type leaching ore deposit bacterium-heterotroph leaching ore deposit bacterium to work in coordination with subsequent leaching sulphide ores, belong to the technical field of Biohydrometallurgy in metallurgical technology.
Background technology
In recent years, along with Mineral resources reduce day by day, and lean ore, abandoned mine and difficulty are adopted, difficult choosing, not only cost is high for useful metal in difficult smelting ore to use traditional technology to extract, and do not meet the environmental requirement of the world today.Valuable element in mineral by number of ways to mineral effect, can be converted into the ion in solution by many microorganisms.Utilize this character of microorganism, in conjunction with related process such as hydrometallurgys, define biological metallurgy technology.The technology of useful metal in Microorganism Leaching ore is utilized to show the uniqueness of its technique, superiority and the feature of environmental protection, by as the important utilization of in metallurgical industry field.
At present about with microbial leaching with utilize the metal ion in microorganism adsorption or conversion industry waste water to do large quantifier elimination, leaching microbacteria mainly contain thiobacillus ferrooxidant (
thiobacillus ferrooxidans,be called for short T.f bacterium), thiobacillus thiooxidans (
thiobacillus thiooxidants,be called for short T.t bacterium), grate sulfur thiobacillus (
thiobacillus thioparus sp.), sulfuration genus bacillus (
sulfobacillus), iron protoxide Ferrobacillus (
ferrobacillus ferrooxidants,be called for short F.f bacterium), leptospirillum belong to (
leptospirillum), the micro-Helicobacter pylori of iron protoxide (
leptospirum ferrooxidans,be called for short L.f bacterium), hyperthermoacidophilic crenarcheon (
thermoacidophilicarchae bacteria) etc.Concerns about bio metallurgy report thiobacillus ferrooxidant (
thiobacillus ferrooxidans) account for the overwhelming majority for the paper of ore immersion bacterial culture, but from investigator to the separation of ore-leaching bacteria and cultural method, should be the enrichment mixed bacterium of multiple bacterial classification.Their some growths are at normal temperature environment, and some then can grow under 50 ~ 70 DEG C or higher temperature.Ferrous ion and elementary sulfur and related compound thereof can be produced in sulphide ores oxidising process, leaching microbacteria is generally from oxygen type bacterium, they obtain energy with the element such as iron protoxide or elementary sulfur and related compound thereof, absorb the oxygen in air and carbonic acid gas, and metal ion in absorbent solution and other desired substance, complete the cycling deposition of self.In recent years, the synergy of microorganism more and more attracts much attention in leaching ore deposit, find that the synergy of the not only autotrophic type such as sulfur-oxidizing bacteria and iron-oxidizing bacterium microorganism makes leaching ore deposit efficiency be greatly improved, and heterotroph microorganism such as
acidiphilium sp.,
alicyclo-bacillus sp. wait the effect in bioleaching system also more and more to attract much attention.Also possess some special knowledge about the effect of heterotrophic bacterium in bioleaching system such as heterotroph aspergillus niger, Penicillium notatums in addition.But almost also not about utilizing the collaborative research of soaking ore deposit mutually of the peculiar physiological property of autotrophy-heterotroph institute.
Autotrophic type leaching ore deposit bacterium: be the special autotrophic type microorganism survived in a kind of Mine water flow that heavy metal ion even can be had to exist at strongly-acid.Such bacterium in Growth and reproduction process with simple inorganics for raw material, as utilized CO
2, CO
3 2-deng as carbon source, utilize N
2, NH
3, NO
2 -, NO
3 -deng as nitrogenous source, obtain electronics energy collecting by the reduction decomposition of the compound such as oxidation, sulfide of the element such as iron, sulphur, thus carry out growth and breeding.As thiobacillus ferrooxidans, as aerobic autotrophic bacterium, obligate addicted to acid, using the sulphur compound of sulphur simple substance and other reductibility as electron donor, with CO
2be that Material synthesis thalline carries out growth and breeding with ammonia; Thiobacillus ferrooxidant is with Oxidation of Fe
2+for Fe
3+obtain the energy to grow in containing mineral salts strong acid medium; Grate sulfur thiobacillus obtains energy: Na by sulfur oxide thiosulfate
2s
2o
3, Na
2sO
4.But this kind of bacterium be easily subject to its own metabolism product restraining effect and can not continuous and effective carry out growth metabolism and breeding, belong to the producer in biologic chain.And the streptomycete of facultative autotrophy type have utilize sulphur and sulfide autotrophy life and utilize organism to carry out the ability of heterotrophism.
Heterotrophic bacterium: it is raw material that such bacterium must obtain gas chromatography from external environment, as protein, carbohydrate etc., ability synthesis bacterium Body components also obtains energy, many heterotrophism acidophilic bacterias and autotrophic bacteria association, carry out metabolism using the products of cellular metabolism of autotrophic bacteria and dead thalline as carbon source and the energy.Also have some heterotrophism acidophilic bacterias can obtain energy by redox iron, and this can produce some inorganic or organic complexs in metabolism, forms complexing ion, enter solution with metal.Such as, chromobacterium violaceum and Rhodopseudomonas produce the HCN that can dissolve gold and silver; Bacillus, fungi, black-koji mould, actinomycetes, gold-coloured staphylococci etc. can generate multiple amino acids, dissolve with after golden complexing.
Be disclose in CN 101434917 to be a kind ofly addicted to leaching-ore bacteria and for the biological dump leaching process of mid low grade phosphate rock at publication number: raw ore is mixed with appropriate pyrite and tensio-active agent after fragmentation, substantially increases the leaching yield of phosphorus.After leach liquor deironing, phosphorus is (with P
2o
5meter) content be greater than 30%, directly can be used for producing that phosphate fertilizer this technical process is short, equipment simple, reduced investment, cost are low, pollution-free, the rate of recovery is high, industrial scale is changeable, the reluctant Mid-and low-grade phosphate rock resources of traditional dressing and smelting process can be processed, improve the utilising efficiency of phosphor resource.
Chinese patent CN 101434918 discloses a kind of leaching ore deposit bacterium and for chalcopyrite ore high temperature heap bioleaching process is: use a kind of Thermophilic Bacterium, by chalcopyrite ore through coarse crushing, in small, broken bits and containing the ore particle obtaining applicable biological dump leaching after the pre-treatment of bacterium solution spraying.Ore particle is through building heap, and dump leaching sprays, and leach liquor circulates, the operations such as rich copper liquid extracting-back extraction-electrodeposition, obtains the cathode copper that market can be sold.Have employed at heap leaching process and comprise normal temperature bacterium, moderate thermophilic bacteria and Thermophilic Bacterium, use and carry out stage by stage, containing bacterium spray working method, making to pile interior temperature and progressively improving.Final maintenance stock dump temperature 10 ~ 80 DEG C, pH value 1.2 ~ 1.8 and suitable Fe concentration are conducive to the activity of ore-leaching bacteria.
Chinese patent CN 101434920 discloses an a kind of strain of low temperature leaching-ore bacteria and for the low temperature heap leaching technique of nickel sulfide ore is: low temperature leaching-ore bacteria rejuvenation domestication and amplification culture, ore builds heap, to drench containing low temperature leaching-ore bacteria drop, leach operation leach liquor out and deliver to deironing arsenic removal and metal recovery operation.This invention can be used for developing the not available low ore grade nickel ore resources of traditional selecting and smelting technology, be especially suitable for the exploitation that normal temperature and high temperature heap bioleaching are difficult to realize the nickel sulfide ore resource of the cold low temp area of height of through engineering approaches, what expand nickel Mineral resources utilizes scope, improves the comprehensive utilization ratio of nickel.
Chinese patent CN 1827805 discloses one and utilizes thermoacidophile process sulphide ores technology, comprise following process steps: (1) bacteria selection and domestication: the thermoacidophile choosing high temperature resistant, resistance to low ph value, by this thermoacidophile after the domestication of ultraviolet mutagenesis, high density sulphide ores and arsenide, screening obtains high-performance bio oxidation bacterial strain further; (2) the spreading cultivation of bacterial classification: high-performance bio is oxidized bacterial strain and is seeded to and spreads cultivation in the seed culture medium of main substrate using the sulphide ores breeze of 3 ~ 15g/L elementary sulfur and 5 ~ 150g/L, obtain cell concentration and reach 1 × 10
8the inoculation liquid of individual/more than L, culture temperature 60 ~ 75 DEG C, incubation time 3 ~ 6 days, medium pH value 1.0 ~ 2.0; (3) the bioleaching sulfide ores process of sulphide ores: productive culture base mixing inoculation liquid, sulphide ores breeze and applicable thermoacidophile grown, carries out the bioleaching sulfide ores reaction of sulphide ores, temperature of reaction 60 ~ 75 DEG C, pH value 1.0 ~ 2.0; Through reaction, when the cupric ion stripping more than 70% of chalcopyrite in sulphide ores breeze, or when pyritous crystalline network more than 70% is destroyed, termination reaction.
Chinese patent CN 1737116 discloses a kind of method utilizing Thiobacillus microorganism to make phosphorus in phosphorus ore be converted into Leaching Properties of Soluble Phosphorus, it is characterized in that it comprises the steps: (1) and activates thiobacillus, obtain active bacteria liquid; (2) in mass ratio for granularity is respectively 40 ~ 200 object pyritic ashess and ground phosphate rock mixing by the ratio of 1:2.8 ~ 3.2, load reaction culture medium, the mass ratio of reaction culture medium and pyritic ashes and ground phosphate rock is 1:10 ~ 30; (3) active bacteria liquid is inoculated in reaction culture medium, the add-on of active bacteria liquid is 5 ~ 25% of the volume of reaction culture medium, constant-temperature shaking culture 24 ~ 240 hours under 60 ~ 180 revs/min of conditions, and cultivating pH is 1.5 ~ 3.0, culture temperature 20 ~ 35 DEG C, obtains Leaching Properties of Soluble Phosphorus.
Because the ore-leaching bacteria of single type just make use of the original characteristic of this bacterium to the effect of mineral to the immersion Zn of mineral, when the biomass in solution reaches some amount, flora growth arrives lag phase and then enters the paracme, and leaching ore deposit process cannot be made to maintain the strongest logarithmic phase of bacterial activity always.And in the later stage in bacterial growth cycle, the secretory product of bacterium may be wrapped in mineral surface, stop bacterium to the further immersion Zn of mineral, the leaching ability of bacterium to mineral is declined greatly, leaching yield reduces.If but utilize autotrophic type to soak the commensalism of ore deposit bacterium and heterotrophic bacterium, them are allowed mutually to act synergistically, bacterium can utilize original chemical element in ore pulp to carry out the growth metabolism of self in such autotrophic type leaching ore deposit, peculiar bacterium or mixotrophism bacterium utilize organism and the refuse of autotrophic type leaching ore deposit bacterium metabolism in system simultaneously, remove the restraining effect to autotrophic type leaching ore deposit bacteria growing, accelerate the growth metabolism of autotrophic type leaching ore deposit bacterium, breeding also just accelerates the oxygenolysis of mineral soon, also improves leaching rate and efficiency.In the system of whole leaching ore deposit, the power of microbial activities and the effect to mineral thereof, the process of the overall complicated Metabolic activity of microorganism, comprise the mineral element such as ferric oxide, sulphur, the compounds such as reduction decomposition sulfide, the slime layer etc. that oxygenolysis organism, Extracellular polymers are formed, the fixing growth elements such as carbon source, nitrogenous source, these processes are all by the synergistic result of various microorganism substantially.
Summary of the invention
The object of this invention is to provide the method utilizing autotrophic type leaching ore deposit bacterium-heterotroph leaching ore deposit bacterium to work in coordination with subsequent leaching sulphide ores.
First in culture system, cultivate obligate or facultative autotrophy type leaching microbacteria, treat that obligate/facultative autotrophy type leaching microbacteria starts to produce enzyme, produce acid, during secretion meta-bolites etc., in culture system, add obligative heterotrophic type leaching microbacteria again cultivate, utilize the various enzymes that obligate/facultative autotrophy type leaching microbacteria produces, organic acid, metabolite, dead thalline, mucus secreted by extracellular polymeric etc. grow necessary nutritive substance for obligative heterotrophic type leaching microbacteria provides to survive, and consumed in culture system obligate/inhibiting material of facultative autotrophy type leaching microbacteria growth generation by the life growth of obligative heterotrophic type leaching microbacteria, promote the growth and breeding of obligate obligate/facultative autotrophy type leaching microbacteria.So carry out, be that whole culture system forms a circulating system, continuous service can be able under the condition without the need to external world's extra-nutrition element.Again such Cyclic culture system is applied to, in microbial leaching technique, reduce the running cost of whole technique, improve the leaching ore deposit efficiency of technique.Its concrete technology step is: a kind of method utilizing autotrophic type leaching ore deposit bacterium-heterotroph leaching ore deposit bacterium to work in coordination with subsequent leaching sulphide ores, in turn includes the following steps:
a,the cultivation of composite mineral-leaching bacterial classification: first with the growth desired substance element of obligate/facultative autotrophy type leaching microbacteria preparation seed culture medium, culture temperature 20 DEG C ~ 85 DEG C, incubation time 12h ~ 120h, medium pH value 0.5 ~ 7.0, in the ratio inoculation culture obligate autotrophy type of 5% ~ 25% or facultative autotrophy type leaching microbacteria under the conditions such as rotating speed 100rpm ~ 200 rpm, by the time it starts to produce various enzyme, organic acid, metabolite having inoculates obligative heterotrophic type or facultative heterotroph microorganism when being attended by dead thalline, with this understanding the bacterial strain of two or more Different Nutrition modes is carried out mixing domestication to cultivate,
b,the preparation of leaching ore deposit substratum: by sulphide ores sample ore break process be the particle of about 1mm and according to rare HCl that the ratio concentration that solid-to-liquid ratio is 1:2 is 2%, immersion treatment carried out 12 hours to it; Then by the sample ore after immersion treatment with protection against corrosion grinding of ball grinder extremely
and the solid-to-liquid ratio preparation seed culture medium of v/v (5% ~ 30%) is pressed as substrate, regulate medium pH value 0.5 ~ 7.0.Wherein sulphide ores sample ore is one or more in the sulphide oress such as copper glance, purple copper, cubanite, covellite, pyrite, corallite, chalcopyrite, molybdenum glance, pyrrhotite, nickel pyrrhotite, violarite, zink sulphide, nickel sulfide ore, manganese glance;
c,composite mineral-leaching bacterial classification leaching ore deposit: the high efficiency composition ore leaching strain after mixing domestication is cultivated is seeded to pretreated breeze as in the seed culture medium of main substrate, leaching ore deposit system pH is regulated to be 0.5 ~ 7.0, temperature 20 DEG C ~ 85 DEG C, cultivate 24h ~ 240h under the condition of rotating speed 100rpm ~ 200 rpm, carry out the bioleaching reaction of sulphide ores.Simultaneously in the sulfide mineral leaching ore body system not having semiconductor property, add semi-conductor sulfide mineral, utilize its semiconductor property, in the process of conduction band electron transition, realize the object of catalysis microbial leaching to accelerate the reaction of leaching ore deposit;
d,the extraction process of metal ion: after reaction, when in sulfuration breeze digestion of metallic ion more than 70% time termination reaction, eliminate the electrostatic adsorption between ore-leaching bacteria and mineral with dilute sulphuric acid adjustment below pH to 1.8, after using heavy liquid separation method sedimentation 100min ~ 130min, slag and the liquid portion containing metal ion, microorganism are separated; Then proceed to cultivate 12h ~ 240h to complete microorganism to metal biosorption inrichment to the mining bacterium of inoculation leaching in the solution after derivation, stop this enrichment reaction when microorganism in system reaches more than 70% metal biosorption accumulation rate, finally add microbial flocculant carry out flocculating-centrifugal-dewatering-burning obtains metal oxide mixture.
innovative point of the present invention is:utilize the physiological property of obligate/facultative autotrophy type leaching microbacteria and obligative heterotrophic type leaching microbacteria, allow both in the process of leaching ore deposit, improve leaching ore deposit efficiency by synergy.
the present invention has following characteristics compared with the method for existing common leaching ore deposit: utilize the physiological property of obligate/facultative autotrophy type leaching microbacteria and obligative heterotrophic type leaching microbacteria to study their collaborative leaching ore deposit action effect first
propose the method that autotrophic type leaching ore deposit bacterium-heterotroph leaching ore deposit bacterium works in coordination with subsequent leaching sulphide ores first, well solve in the process of leaching ore deposit and occur lag phase, the low such phenomenon of bacterium utilization ratio
autotrophic type leaching ore deposit bacterium-heterotroph leaching bacterium synergistic whole leaching ore deposit, ore deposit systemic circulation is carried out, make use of semi-conductor sulfide mineral can provide electronics to promote the characteristic of microbial leaching effect in the process of transition of electron, be more conducive to the leaching of metallic element in sample ore, be beneficial to and improve leaching yield, reduction leaching ore deposit cost.
Embodiment
Here is several concrete embodiment of the present invention; so that the invention will be further described; but can not be interpreted as it is limiting the scope of the invention; some nonessential improvement and adjustment that person skilled in art makes the present invention according to the content of the invention described above, still belong to protection scope of the present invention.
example table 1
.
example table 2
。
In the present invention, (1) is soaked mining Strain type and is mainly contained following several:
Obligate autotrophy type leaching microbacteria is: thiobacillus ferrooxidant (
thiobacillus ferrooxidans), Acidithiobacillus ferrooxidans strain GF (
acidithiobacillus ferrooxidans), grate sulfur thiobacillus (
thiobacillus thioparus), iron protoxide Ferrobacillus (
ferrobacillus ferrooxidans), hyperthermoacidophilic crenarcheon (
thermoacidophilicarchae bacteria), thermophilic acidophilic acid sulfolobus solfataricus (
sulfolobus acidocaldarius), ten thousand sour bacterium (
acidianus manzaensis), Thermophilic Bacterium (
sulfolobus metallicus), thermophilic metal coccus (
metallosphaera sedula), thiobacillus thiooxidans (
acidithiobacillus thiooxidans), leptospirillum ferriphilum (
leptospirillum ferriphilum), iron protoxide hook end spirobacteria (
leptospirillum ferrooxidans),
ferroplasma acidiphilum,
leptospirillum thermoferrooxidans,
acidiphilium acidophilumdeng one or more in microorganism.
Facultative autotrophy type leaching microbacteria is: extreme thermophile bacterium (
acidianus brierleyi), addicted to the ancient bacterium of ultrahigh-temperature (
sulfolobus solfataricus), hold concurrently support bacillus acidophilus (
acidiphilium sPP.), facultatively autotrophic streptomyces (
streptomyces avermitilis), thermophilic thiobacillus (
acidithiobacillus caldus), sulfobacillus thermosulfidooxidans (
sulfobacillus thermosulfidooxidans), addicted to sour Sulfobacillus (
sulfobacillus acidophilus), thermoplasma acidophilum (
thermoplasma acidophilum), diligent metal coccus (
metallosphaera sedula) etc. one or more in microorganism.
Heterotroph leaching microbacteria is: thermophilic acidophilic acid genus bacillus (
bacillus acidocaldarius), addicted to acid heat heterotrophic bacterium TC-2, rhodotorula (
rhodotorula spp.), candiyeast (
candida), Cryptococcus (
cryptococcus); In filamentous fungus
acontiumcy latium,
tichosporone crebriae, Cephalosporium sP; Bacillus acidophilus belong in hiding acidophilic bacteria (
acidiphilium cryptum), red acidophilic bacteria (
acidiphilium rubrum), eat more acidophilic bacteria (
acidiphilium multivorum), bite organic acidophilic bacteria (
acidiphilium organovorum), separate amine acidophilic bacteria (
acidiphilium aminolytica), quick acidophilic bacteria (
acidiphilium facile);
acidiphilium acidophilum,
sulfobacillus sulfidooxidans,
ferroplasma acidarmanus,
ferroplasma cupricumulans, Sulfolobus acidocaldarius, Sulfurisphaera ohwakuensisdeng one or more in microorganism.
(2) to soak ore deposit pH value range accordingly as follows for all types of leaching microbacteria:
1. be that the microorganism can soaking ore deposit between 0.5 ~ 1.0 has in pH value:
In obligate autotrophy type leaching microbacteria: hyperthermoacidophilic crenarcheon (
thermoacidophilicarchae bacteria),
ferroplasma acidiphilum;
In facultative autotrophy type leaching microbacteria: thermoplasma acidophilum (
thermoplasma acidophilum)
2. be that the microorganism can soaking ore deposit between 1.0 ~ 2.0 has in pH value:
In obligate autotrophy type leaching microbacteria: thermophilic acidophilic acid sulfolobus solfataricus (
sulfolobus acidocaldarius), ten thousand sour bacterium (
acidianus manzaensis), thermophilic metal coccus (
metallosphaera sedula), leptospirillum ferriphilum (
leptospirillum ferriphilum),
leptospirillum thermoferrooxidans, Acidiphilium acidophilum;
In facultative autotrophy type leaching microbacteria: addicted to the ancient bacterium of ultrahigh-temperature (
sulfolobus solfataricus), thermoplasma acidophilum (
thermoplasma acidophilum), diligent metal coccus (
metallosphaera sedula);
In heterotroph leaching microbacteria: addicted to acid heat heterotrophic bacterium TC-2,
sulfobacillus sulfidooxidans,
ferroplasma acidarmanus,
ferroplasma cupricumulans,
sulfurisphaera ohwakuensis
3. be that the microorganism can soaking ore deposit between 2.0 ~ 4.0 has in pH value:
In obligate autotrophy type leaching microbacteria: thiobacillus ferrooxidant (
thiobacillus ferrooxidans), Acidithiobacillus ferrooxidans strain GF (
acidithiobacillus ferrooxidans), thiobacillus thiooxidans (
acidithiobacillus thiooxidans), grate sulfur thiobacillus (
thiobacillus thioparus), iron protoxide Ferrobacillus (
ferrobacillus ferrooxidans), thermophilic acidophilic acid sulfolobus solfataricus (
sulfolobus acidocaldarius),
acidiphilium acidophilum,iron protoxide hook end spirobacteria (
leptospirillum ferrooxidans);
In facultative autotrophy type leaching microbacteria: sulfolobus acidocaldarius (
sulfolobus acidocaldarius), addicted to the ancient bacterium of ultrahigh-temperature (
sulfolobus solfataricus), facultative autotrophy type bacillus acidophilus (
acidiphilium sPP.), thermophilic thiobacillus (
acidithiobacillus caldus), sulfobacillus thermosulfidooxidans (
sulfobacillus thermosulfidooxidans), addicted to sour Sulfobacillus (
sulfobacillus acidophilus)
In heterotroph leaching microbacteria: addicted to acid heat heterotrophic bacterium TC-2, thermophilic acidophilic acid genus bacillus (
bacillus acidocaldarius), hide acidophilic bacteria (
acidiphilium cryptum),
acidiphilium acidophilum, red acidophilic bacteria (
acidiphilium rubrum), eat more acidophilic bacteria (
acidiphilium multivorum), bite organic acidophilic bacteria (
acidiphilium organovorum), separate amine acidophilic bacteria (
acidiphilium aminolytica), quick acidophilic bacteria (
acidiphilium facile).
4. be that the microorganism can soaking ore deposit between 4.0 ~ 7.0 has in pH value:
In facultative autotrophy type leaching microbacteria: sulfolobus acidocaldarius (
sulfolobus acidocaldarius), streptomycete (
streptomyces avermitilis).
In heterotroph leaching microbacteria: thermophilic acidophilic acid genus bacillus (
bacillus acidocaldarius), black-koji mould
(Aspergillus), rhodotorula (
rhodotorula spp.), candiyeast (
candida), Cryptococcus (
cryptococcus),
acontiumcy latium,
tichosporone crebriae,
cephalosporium. sP.
Each embodiment data listed by upper table, can produce product of the present invention by the step described by technical scheme part.
Claims (5)
1. utilize autotrophic type to soak ore deposit bacterium-heterotroph and soak the method that ore deposit bacterium works in coordination with subsequent leaching sulphide ores, in turn include the following steps:
A, the cultivation of composite mineral-leaching bacterial classification: first with the growth desired substance element of obligate/facultative autotrophy type leaching microbacteria preparation seed culture medium, culture temperature 20 DEG C ~ 85 DEG C, incubation time 12h ~ 120h, medium pH value 0.5 ~ 7.0, in the ratio inoculation culture obligate autotrophy type of 5% ~ 25% or facultative autotrophy type leaching microbacteria under rotating speed 100rpm ~ 200rpm condition, by the time it starts to produce various enzyme, organic acid, metabolite having inoculates obligative heterotrophic type or facultative heterotroph microorganism when being attended by dead thalline, with this understanding the bacterial strain of two or more Different Nutrition modes is carried out mixing domestication to cultivate,
The preparation of B, leaching ore deposit substratum: by sulphide ores sample ore break process be the particle of about 1mm and according to rare HCl that the ratio concentration that solid-to-liquid ratio is 1:2 is 2%, immersion treatment carried out 12 hours to it; Then using the sample ore protection against corrosion grinding of ball grinder to 74 after immersion treatment μm ~ 104 μm and the solid-to-liquid ratio preparation seed culture medium being 5% ~ 30% by v/v as substrate, medium pH value 0.5 ~ 7.0 is regulated;
C, composite mineral-leaching bacterial classification leaching ore deposit: the high efficiency composition ore leaching strain after mixing domestication is cultivated is seeded to pretreated breeze as in the seed culture medium of main substrate, leaching ore deposit system pH is regulated to be 0.5 ~ 7.0, temperature 20 DEG C ~ 85 DEG C, cultivate 24h ~ 240h under the condition of rotating speed 100rpm ~ 200rpm, carry out the bioleaching reaction of sulphide ores; In above-mentioned reaction, there is no transition of electron simultaneously and do not have in the sulfide mineral leaching ore body system of conductivity, be added in the sulfide mineral that conduction band conducts electricity very well because transition of electron has, utilize its conductivity, realize the object of catalysis microbial leaching, to accelerate the reaction of leaching ore deposit;
The extraction process of D, metal ion: after reaction, when in sulfuration breeze digestion of metallic ion more than 70% time termination reaction, eliminate the electrostatic adsorption between ore-leaching bacteria and mineral with dilute sulphuric acid adjustment below pH to 1.8, after using heavy liquid separation method sedimentation 100min ~ 130min, slag and the liquid portion containing metal ion, microorganism are separated; Then proceed to cultivate 12h ~ 240h to complete microorganism to metal biosorption inrichment to the mining bacterium of inoculation leaching in the solution after derivation, stop this enrichment reaction when microorganism in system reaches more than 70% metal biosorption accumulation rate, finally add microbial flocculant carry out flocculating-centrifugal-dewatering-burning obtains metal oxide mixture.
2. the method utilizing autotrophic type leaching ore deposit bacterium-heterotroph leaching ore deposit bacterium to work in coordination with subsequent leaching sulphide ores according to claim 1, it is characterized in that, soak in mining composite mineral-leaching bacterial classification and must have obligate/facultative autotrophy type leaching microbacteria and obligative heterotrophic type leaching microbacteria simultaneously.
3. the method utilizing autotrophic type leaching ore deposit bacterium-heterotroph leaching ore deposit bacterium to work in coordination with subsequent leaching sulphide ores according to claim 1, it is characterized in that, the leaching ore deposit pH value range of all types of composite mineral-leaching microorganism is 0.5 ~ 7.0.
4. the method utilizing autotrophic type leaching ore deposit bacterium-heterotroph leaching ore deposit bacterium to work in coordination with subsequent leaching sulphide ores according to claim 1, it is characterized in that, the leaching ore deposit pH value range of all types of composite mineral-leaching microorganism is 0.5 ~ 7.0, and the reagent of adjust ph is dilute sulphuric acid, acetic acid and sodium bicarbonate, sodium carbonate, ammoniacal liquor, calcium hydroxide.
5. the method utilizing autotrophic type leaching ore deposit bacterium-heterotroph leaching ore deposit bacterium to work in coordination with subsequent leaching sulphide ores according to claim 1, it is characterized in that, at claim 1 temperature 20 DEG C ~ 85 DEG C, add in cubanite, purple copper, pyrrhotite, manganese glance, covellite, violarite, nickel sulfide ore in sulfide mineral composite bacteria leaching ore body system one or more, adding powder particle size scope is 74 μm ~ 104 μm, and add-on is 8% ~ 15% of sulphide ores total amount in former leaching ore body system.
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| CN103866123A (en) * | 2014-04-07 | 2014-06-18 | 张在海 | Method for leaching valuable metals at high temperature by using acidophilus archaea |
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