CN102329957A - Method for continuously leaching sulfide ore by using synergy of autotrophic ore leaching bacteria and heterotrophic ore leaching bacteria - Google Patents
Method for continuously leaching sulfide ore by using synergy of autotrophic ore leaching bacteria and heterotrophic ore leaching bacteria Download PDFInfo
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Abstract
The invention discloses a method for continuously leaching sulfide ore by using the synergy of autotrophic ore leaching bacteria and heterotrophic ore leaching bacteria, which comprises two main steps of preparing a compound ore leaching strain and leaching ore by using the compound strain. The step of preparing the compound ore leaching strain comprises the steps of selecting a strain, preparing a culture medium, compositely culturing the strain and domesticating the compound ore leaching strain. The step of leaching ore by using the compound strain comprises the steps of pretreating an ore sample, preparing an ore leaching culture medium, leaching the ore by using the compound ore leaching strain, selecting and adding semiconductor sulfide ore and extracting metals in the leaching liquids. In the method, the characteristic of the semiconductor sulfide ore to provide electrons to promote the ore leaching action of microbes in the electron transition process is utilized, so that the operating cost of the whole process is lowered, the ore leaching efficiency of the process is improved, and the method has broad application prospect in the field of metallurgy.
Description
Technical field
The present invention relates to utilize autotrophic type to soak the method that ore deposit bacterium-heterotroph soaks the collaborative subsequent leaching sulphide ores of ore deposit bacterium, belong to the technical field of biological hydrometallurgy in the metallurgical technology.
Background technology
In recent years, along with Mineral resources reduce day by day, and the utilization traditional technology is extracted that lean ore, abandoned mine and difficult adopted, not only cost is high for the useful metal in the difficult choosing, difficult smelting ore, and does not satisfy the environmental requirement of the world today.Many mikrobes can be converted into the ion in the solution with the valuable element in the mineral through number of ways to the mineral effect.Utilize this character of mikrobe,, formed the biological metallurgy technology in conjunction with related process such as hydrometallurgys.The technology of utilizing mikrobe to leach useful metal in the ore has demonstrated uniqueness, meliority and the feature of environmental protection of its technology, is used as an important utilization in the metallurgical industry field.
At present about with microbial leaching with utilize the metals ion in mikrobe absorption or the conversion trade effluent to do a large amount of research, leaching microbacteria mainly contain thiobacillus ferrooxidant (
Thiobacillus ferrooxidans,Be called for short the T.f bacterium), thiobacillus thiooxidans (
Thiobacillus thiooxidants,Be called for short the T.t bacterium), grate sulfur thiobacillus (
Thiobacillus thioparus sp.), the sulfuration genus bacillus (
Sulfobacillus), the iron protoxide Ferrobacillus (
Ferrobacillus ferrooxidants,Be called for short the F.f bacterium), leptospirillum belong to (
Leptospirillum), the little Helicobacter pylori of iron protoxide (
Leptospirum ferrooxidans,Be called for short the L.f bacterium), the hyperthermoacidophilic crenarcheon (
Thermoacidophilicarchae bacteria) etc.The report thiobacillus ferrooxidant of relevant biological metallurgy (
Thiobacillus ferrooxidans) for the paper of ore immersion bacterial culture accounts for the overwhelming majority, but from separation and the cultural method of investigator to ore-leaching bacteria, should be the enrichment mixed bacterium of a plurality of bacterial classifications.They are grown in normal temperature environment a bit, and some then can growth under 50～70 ℃ or higher temperature.Can produce ferrous ion and elementary sulfur and related compound thereof in the sulphide ores oxidising process; Leaching microbacteria is generally from oxygen type bacterium; They are with element and related compound acquisition energy thereof such as iron protoxide or elementary sulfurs; Absorb airborne oxygen and carbonic acid gas, and the metals ion in the absorbent solution and other desired substance, accomplish the cycling deposition of self.In recent years, the synergy of mikrobe is being soaked the attention that more and more causes people aspect the ore deposit, find that not only the synergy of autotrophic type such as sulfur-oxidizing bacteria and iron-oxidizing bacterium mikrobe makes that soaking ore deposit efficient is greatly improved, and the heterotroph mikrobe for example
Acidiphilium sp.,
Alicyclo-bacillus sp. wait the effect in the bioleaching system also more and more to cause people's attention.Also possess some special knowledge about the effect of heterotrophic bacterium in the bioleaching system such as heterotroph black mold, penicillium spps in addition.But almost also not about utilizing the peculiar physiological property of autotrophy-heterotroph institute to work in coordination with the research of soaking the ore deposit each other.
Autotrophic type soaks the ore deposit bacterium: be a kind of special autotrophic type mikrobe that can in strongly-acid even the pit waste water that has heavy metal ion to exist, survive.Such bacterium is a raw material with simple inorganics in growth and reproductive process, as utilizes CO
2, CO
3 2-Deng as carbon source, utilize N
2, NH
3, NO
2 -, NO
3 -Deng as nitrogenous source, the reduction decomposition electron gain energy collecting of compound such as the oxidation through elements such as iron, sulphur, sulfide, thus carry out growth and breeding.Like the thiobacillus ferrooxidans, as aerobic autotrophic bacteria, obligate is had a liking for acid, with the sulphur compound of sulphur simple substance and other reductibility as electron donor, with CO
2With ammonia be that the synthetic thalline of raw material carries out growth and breeding; Thiobacillus ferrooxidant is with Oxidation of Fe
2+Be Fe
3+Obtaining the energy grows in containing mineral salts strongly-acid medium; Grate sulfur thiobacillus leans on the sulfur oxide thiosulfate to obtain energy: Na
2S
2O
3, Na
2SO
4But this type bacterium receive easily it self meta-bolites restraining effect and can not continuous and effective carry out growth metabolism and breeding, belong to the producer in the biologic chain.And the streptomycete of facultative autotrophy type has the sulphur of utilization and the life of sulfide autotrophy and utilizes organism to carry out heterotrophic ability.
Heterotrophic bacterium: such bacterium must obtain multiple organism from external environment be raw material; Like protein, carbohydrate etc.; Could synthesize the thalline composition and obtain energy; Many heterotrophism wtihs and autotrophic bacteria association are carried out metabolism with the products of cellular metabolism of autotrophic bacteria and the thalline of death as the carbon source and the energy.Also have some heterotrophism wtihs to obtain energy through redox iron, and these some inorganic or organic complexs of meeting generation in metabolism, form complexing ion with metal, get into solution.For example, chromobacterium violaceum and Rhodopseudomonas produce the HCN that can dissolve gold and silver; Bud pole Pseudomonas, fungi, black-koji mould, actinomycetes, gold-coloured staphylococci etc. can generate multiple amino acids, and dissolve after the golden complexing.
At publication number is to disclose a kind of biological dump leaching process of having a liking for acidleach ore deposit bacterium and being used for mid low grade phosphate rock among the CN 101434917 to be: raw ore is mixed with an amount of pyrite and tensio-active agent after fragmentation, has improved the leaching yield of phosphorus greatly.After the leach liquor deironing, phosphorus is (with P
2O
5Meter) content is greater than 30%; Can be used for directly producing that this technical process of phosphate fertilizer is short, equipment is simple, reduced investment, cost is low, pollution-free, the recovery is high; Industrial scale is changeable; Can handle the reluctant mid low grade phosphate rock resource of traditional selecting smelting process, improve the utilising efficiency of phosphor resource.
Chinese patent CN 101434918 disclose a kind of soak the ore deposit bacterium and be used for the chalcopyrite ore high temperature heap bioleaching process be: use a kind of extreme thermophile bacteria, with the chalcopyrite ore through coarse crushing, in small, broken bits and contain the pre-treatment of bacterium solution spraying after obtain being fit to the ore particle of biological dump leaching.Ore particle is through building heap, dump leaching spray, leach liquor circulation, operations such as rich copper liquid extracting-back extraction-electrodeposition, the cathode copper that acquisition market can be sold.Adopted at heap leaching process to comprise normal temperature bacterium, moderate thermophilic bacteria and extreme thermophile bacteria, utilization contains bacterium spray working method stage by stage, makes that temperature progressively improves in the heap.The final activity that keeps 10～80 ℃ of stock dump temperature, pH value 1.2～1.8 and proper Fe concentration to help ore-leaching bacteria.
The low temperature heap leaching technology that Chinese patent CN 101434920 discloses an a kind of strain of low temperature leaching-ore bacteria and has been used for nickel sulfide ore is: low temperature leaching-ore bacteria rejuvenation domestication and amplification culture; Ore is built heap; Drench to contain the low temperature leaching-ore bacteria drop, the leach liquor that the leaching operation is come out is delivered to deironing arsenic removal and metal recovery operation.This invention can be used for developing traditional selecting smelting tech low ore grade nickel ore resources not capable of using; Especially be suitable for normal temperature and high temperature heap bioleaching and be difficult to realize the exploitation of nickel sulfide ore resource of the high cold low temp area of through engineering approaches; Enlarge the scope of utilizing that nickel minerals produces resource, improve the comprehensive utilization ratio of nickel.
Chinese patent CN 1827805 discloses a kind of thermoacidophile processing sulphide ores technology of utilizing; Comprise following process steps: the screening and the domestication of (1) bacterial classification: the thermoacidophile of choosing high temperature resistant, anti-low pH value; Through after the domestication of ultraviolet mutagenesis, high density sulphide ores and arsenide, further screening obtains high-performance bio oxidation bacterial strain with this thermoacidophile; (2) spreading cultivation of bacterial classification: high-performance bio oxidation inoculation is spread cultivation to the seed culture medium of sulphide ores breeze as main substrate with 3～15g/L elementary sulfur and 5～150g/L, obtain cell concentration and reach 1 * 10
8The inoculation liquid that individual/L is above, 60～75 ℃ of culture temperature, incubation time 3～6 days, medium pH value 1.0～2.0; (3) bio-oxidation of sulphide ores is soaked the ore deposit processing: the production substratum of inoculation liquid, sulphide ores breeze and the growth of suitable thermoacidophile is mixed, carry out the bio-oxidation of sulphide ores and soak ore deposit reaction, 60～75 ℃ of temperature of reaction, pH value 1.0～2.0; Through reaction, the cupric ion stripping of yellow copper ore more than 70% in the sulphide ores breeze, or pyritous crystalline network is when being destroyed more than 70%, termination reaction.
It is to it is characterized in that it comprises the steps: (1) activation thiobacillus, obtains active bacterium liquid that Chinese patent CN 1737116 discloses a kind of method of utilizing the Thiobacillus mikrobe to make phosphorus in the phosphorus ore be converted into solvable phosphorus; (2) be that the ratio of 1:2.8～3.2 is respectively 40～200 purpose pyritic ashess and ground phosphate rock with granularity and mixes in mass ratio, the reaction culture medium of packing into, the mass ratio of reaction culture medium and pyritic ashes and ground phosphate rock are 1:10～30; (3) active bacterium liquid is inoculated in the reaction culture medium; The add-on of active bacterium liquid be reaction culture medium volume 5～25%, constant-temperature shaking culture is 24～240 hours under 60～180 rev/mins of conditions, cultivating pH is 1.5～3.0; 20～35 ℃ of culture temperature obtain solvable phosphorus.
Because the ore-leaching bacteria of single type has just utilized the effect of the original characteristic of this bacterium to mineral to the leaching effect of mineral; When the biomass in the solution reaches some amount; Flora growth arrives lag phase and then gets into the paracme, can't make to soak the ore deposit process and maintain the strongest logarithmic phase of bacterial activity always.And in the later stage in bacterial growth cycle, the secretory product of bacterium may be wrapped in mineral surface, stops the further leaching effect of bacterium to mineral, makes bacterium descend greatly to the leaching ability of mineral, and leaching yield reduces.If but utilize autotrophic type to soak the commensalism of ore deposit bacterium and heterotrophic bacterium; Let them act synergistically each other; Autotrophic type soaks the ore deposit bacterium and can utilize original chemical element in the ore pulp to carry out the growth metabolism of self like this; Simultaneously peculiar bacterium or mixotrophism bacterium utilize that autotrophic type soaks metabolic organism of ore deposit bacterium and refuse in the system, remove the restraining effect that autotrophic type is soaked the ore deposit bacteria growing, accelerate the growth metabolism that autotrophic type soaks the ore deposit bacterium; Breeding has also just been quickened the oxygenolysis of mineral soon, has also improved leaching rate and efficient.Soak in the system of ore deposit whole; Microbial activities strong and weak and to the effect of mineral, the process of the whole complicated Metabolic activity of mikrobe comprises mineral elements such as red stone, sulphur; Compounds such as reduction decomposition sulfide; The slime layer that the outer polymer of oxygenolysis organism, born of the same parents forms etc., fixing growth elements such as carbon source, nitrogenous source, these processes all are by the synergistic result of various mikrobes basically.
Summary of the invention
The purpose of this invention is to provide and utilize autotrophic type to soak the method that ore deposit bacterium-heterotroph soaks the collaborative subsequent leaching sulphide ores of ore deposit bacterium.
At first in culture system, cultivate obligate or facultative autotrophy type leaching microbacteria; Treat that obligate/facultative autotrophy type leaching microbacteria begins to produce enzyme, produces acid, during secretion meta-bolites etc.; In culture system, adding obligate heterotroph leaching microbacteria again cultivates; Utilize the secreted mucus of various enzymes that obligate/facultative autotrophy type leaching microbacteria is produced, organic acid, metabolite, dead thalline, extracellular polymeric etc. to provide the existence growth necessary nutritive substance for obligate heterotroph leaching microbacteria; And, promote the growth and breeding of obligate obligate/facultative autotrophy type leaching microbacteria through in the life growth consumption culture system of obligate heterotroph leaching microbacteria obligate/facultative autotrophy type leaching microbacteria growth being produced inhibiting material.So carrying out, is that whole culture system forms a circulating system, can under the condition that need not extraneous extra-nutrition element, be able to continuous service.Again such circulation culture system is applied to reduce the running cost of whole technology in the microbial leaching technology, that improves technology soaks ore deposit efficient.Its concrete process step is: a kind of autotrophic type that utilizes soaks the method that ore deposit bacterium-heterotroph soaks the collaborative subsequent leaching sulphide ores of ore deposit bacterium, in turn includes the following steps:
A,Composite mineral-leaching culture of strains: prepare seed culture medium with the growth desired substance element of obligate/facultative autotrophy type leaching microbacteria earlier; 20 ℃～85 ℃ of culture temperature; Incubation time 12h～120h; Medium pH value 0.5～7.0; In 5%～25% ratio inoculation culture obligate autotrophy type or facultative autotrophy type leaching microbacteria, it begins to produce various enzymes, organic acid, metabolite and has when being attended by dead thalline to inoculate obligate heterotroph or facultative heterotroph mikrobe by the time under rotating speed 100rpm～conditions such as 200 rpm, and the bacterial strain with two or more Different Nutrition modes mixes acclimation shaking culture with this understanding;
B,Soak the ore deposit culture medium preparation: sulphide ores sample ore break process is the particle about 1mm and is that to use concentration be that rare HCl of 2% carried out immersion treatment 12 hours to it for the ratio of 1:2 according to solid-to-liquid ratio; Then the sample ore after the immersion treatment is milled to the protection against corrosion ball mill
And press the solid-to-liquid ratio preparation seed culture medium of v/v (5%～30%) as substrate, regulate medium pH value 0.5～7.0.Wherein the sulphide ores sample ore is one or more in the sulphide oress such as copper glance, purple copper, cubanite, covellite, pyrite, corallite, yellow copper ore, molybdenum glance, pyrrhotite, nickel pyrrhotite, violarite, zink sulphide, nickel sulfide ore, sulfuration manganese ore;
C,The composite mineral-leaching bacterial classification soaks the ore deposit: will mix in the seed culture medium of efficient composite mineral-leaching inoculation to pretreated breeze as main substrate after the acclimation shaking culture; It is 0.5～7.0 that the ore deposit system pH is soaked in adjusting; 20 ℃～85 ℃ of temperature; Cultivate 24h～240h under the condition of rotating speed 100rpm～200 rpm, carry out the bioleaching reaction of sulphide ores.Soak in the ore body system at the sulfide mineral that does not have semiconductor property simultaneously, add semi-conductor sulfuration mineral, utilize its semiconductor property, the purpose that in the process of conduction band electron transition, realizes the catalysis microbial leaching is to accelerate to soak the ore deposit reaction;
D,The extraction of metals ion is handled: after reaction; Termination reaction when the metals ion stripping 70% of sulfuration in the breeze is above; Regulate the electrostatic adsorption of eliminating below the pH to 1.8 between ore-leaching bacteria and the mineral with dilute sulphuric acid, behind utilization heavy liquid separation method sedimentation 100min～130min slag and the liquid portion that contains metals ion, mikrobe are separated; Inoculation is soaked mining bacterium and is proceeded to cultivate 12h～240h to accomplish the adsorption and enrichment effect of mikrobe to metals ion in the solution after deriving then; Stop this enrichment reaction when mikrobe in the system reaches 70% to the adsorption and enrichment rate of metals ion when above, add at last microbial flocculant flocculate-centrifugal-dehydration-burning to be to obtain metal oxide mixture.
Innovative point of the present invention is:Utilize the physiological property of obligate/facultative autotrophy type leaching microbacteria and obligate heterotroph leaching microbacteria, let both in soaking the ore deposit process, soak ore deposit efficient through the synergy raising.
The present invention has compared following characteristics with the existing common ore deposit method of soaking: 
Utilize the physiological property of obligate/facultative autotrophy type leaching microbacteria and obligate heterotroph leaching microbacteria to study their the collaborative ore deposit action effect that soaks first
Propose autotrophic type first and soak the method that ore deposit bacterium-heterotroph soaks the collaborative subsequent leaching sulphide ores of ore deposit bacterium, well solved to soak and occur lag phase, the low such phenomenon of bacterium utilization ratio in the process of ore deposit
Autotrophic type soaks ore deposit bacterium-heterotroph and soaks the synergistic whole ore deposit systemic circulation of soaking of ore deposit bacterium and carry out; Utilized semi-conductor sulfuration mineral in the process of transition of electron, can provide electronics to promote the characteristic of microbial leaching effect; Be more conducive to the leaching of metallic element in the sample ore, be beneficial to the raising leaching yield, reduce and soak the ore deposit cost.
Embodiment
Be several practical implementation instance of the present invention below; So that the present invention is described further; But can not be interpreted as it is restriction to protection domain of the present invention; The technician in this field to some nonessential improvement and adjustment that the present invention makes, still belongs to protection scope of the present invention according to the content of the invention described above.
Example table 1
Example table 2
(1) is soaked mining bacterial strain type and is mainly contained following several kinds among the present invention:
Obligate autotrophy type leaching microbacteria is: thiobacillus ferrooxidant (
Thiobacillus ferrooxidans), have a liking for the ferrous thiobacillus of acid oxidase (
Acidithiobacillus ferrooxidans), grate sulfur thiobacillus (
Thiobacillus thioparus), the iron protoxide Ferrobacillus (
Ferrobacillus ferrooxidans), the hyperthermoacidophilic crenarcheon (
Thermoacidophilicarchae bacteria), thermophilic acidophilic acid sulfolobus solfataricus (
Sulfolobus acidocaldarius), ten thousand sour bacterium (
Acidianus manzaensis), extreme thermophile bacteria (
Sulfolobus metallicus), thermophilic metal coccus (
Metallosphaera sedula), thiobacillus thiooxidans (
Acidithiobacillus thiooxidans), leptospirillum ferriphilum (
Leptospirillum ferriphilum), iron protoxide hook end spirobacteria (
Leptospirillum ferrooxidans),
Ferroplasma acidiphilum,
Leptospirillum thermoferrooxidans,
Acidiphilium acidophilumDeng in the mikrobe one or more.
Facultative autotrophy type leaching microbacteria is: extreme thermophile bacterium (
Acidianus brierleyi), have a liking for the ancient bacterium of ultrahigh-temperature (
Sulfolobus solfataricus), the bacillus acidophilus that hold concurrently to support (
Acidiphilium sPP.), facultative autotrophy type streptomycete (
Streptomyces avermitilis), thermophilic thiobacillus (
Acidithiobacillus caldus), sulfobacillus thermosulfidooxidans (
Sulfobacillus thermosulfidooxidans), have a liking for acid sulfuration bacillus (
Sulfobacillus acidophilus), the thermoplasma acidophilum (
Thermoplasma acidophilum), diligent metal coccus (
Metallosphaera sedula) wait in the mikrobe one or more.
The heterotroph leaching microbacteria is: thermophilic acidophilic acid genus bacillus (
Bacillus acidocaldarius), have a liking for acid heat heterotrophic bacterium TC-2, rhodotorula (
Rhodotorula spp.), candiyeast (
Candida), Cryptococcus (
Cryptococcus); In the filamentous fungus
Acontiumcy latium,
Tichosporone crebriae, Cephalosporium sPHiding wtih during the bacillus acidophilus belongs to (
Acidiphilium cryptum), red wtih (
Acidiphilium rubrum), eat more wtih (
Acidiphilium multivorum), bite organic wtih (
Acidiphilium organovorum), separate the amine wtih (
Acidiphilium aminolytica), quick wtih (
Acidiphilium facile);
Acidiphilium acidophilum,
Sulfobacillus sulfidooxidans,
Ferroplasma acidarmanus,
Ferroplasma cupricumulans, Sulfolobus acidocaldarius, Sulfurisphaera ohwakuensisDeng in the mikrobe one or more.
(2) to soak ore deposit pH value scope accordingly following for all types of leaching microbacterias:
1. be that the mikrobe that can soak the ore deposit between 0.5～1.0 has in the pH value:
In the obligate autotrophy type leaching microbacteria: the hyperthermoacidophilic crenarcheon (
Thermoacidophilicarchae bacteria),
Ferroplasma acidiphilum
In the facultative autotrophy type leaching microbacteria: the thermoplasma acidophilum (
Thermoplasma acidophilum)
2. be that the mikrobe that can soak the ore deposit between 1.0～2.0 has in the pH value:
In the obligate autotrophy type leaching microbacteria: thermophilic acidophilic acid sulfolobus solfataricus (
Sulfolobus acidocaldarius), ten thousand sour bacterium (
Acidianus manzaensis), thermophilic metal coccus (
Metallosphaera sedula), leptospirillum ferriphilum (
Leptospirillum ferriphilum),
Leptospirillum thermoferrooxidans, Acidiphilium acidophilum
In the facultative autotrophy type leaching microbacteria: have a liking for the ancient bacterium of ultrahigh-temperature (
Sulfolobus solfataricus), the thermoplasma acidophilum (
Thermoplasma acidophilum), diligent metal coccus (
Metallosphaera sedula);
In the heterotroph leaching microbacteria: have a liking for acid heat heterotrophic bacterium TC-2,
Sulfobacillus sulfidooxidans,
Ferroplasma acidarmanus,
Ferroplasma cupricumulans,
Sulfurisphaera ohwakuensis
3. be that the mikrobe that can soak the ore deposit between 2.0～4.0 has in the pH value:
In the obligate autotrophy type leaching microbacteria: thiobacillus ferrooxidant (
Thiobacillus ferrooxidans), have a liking for the ferrous thiobacillus of acid oxidase (
Acidithiobacillus ferrooxidans), thiobacillus thiooxidans (
Acidithiobacillus thiooxidans), grate sulfur thiobacillus (
Thiobacillus thioparus), the iron protoxide Ferrobacillus (
Ferrobacillus ferrooxidans), thermophilic acidophilic acid sulfolobus solfataricus (
Sulfolobus acidocaldarius),
Acidiphilium acidophilum,Iron protoxide hook end spirobacteria (
Leptospirillum ferrooxidans);
In the facultative autotrophy type leaching microbacteria: the sulfolobus acidocaldarius (
Sulfolobus acidocaldarius), have a liking for the ancient bacterium of ultrahigh-temperature (
Sulfolobus solfataricus), facultative autotrophy type bacillus acidophilus (
Acidiphilium sPP.), thermophilic thiobacillus (
Acidithiobacillus caldus), sulfobacillus thermosulfidooxidans (
Sulfobacillus thermosulfidooxidans), have a liking for acid sulfuration bacillus (
Sulfobacillus acidophilus)
In the heterotroph leaching microbacteria: have a liking for acid heat heterotrophic bacterium TC-2, thermophilic acidophilic acid genus bacillus (
Bacillus acidocaldarius), hide wtih (
Acidiphilium cryptum),
Acidiphilium acidophilum, red wtih (
Acidiphilium rubrum), eat more wtih (
Acidiphilium multivorum), bite organic wtih (
Acidiphilium organovorum), separate the amine wtih (
Acidiphilium aminolytica), quick wtih (
Acidiphilium facile).
4. be that the mikrobe that can soak the ore deposit between 4.0～7.0 has in the pH value:
In the facultative autotrophy type leaching microbacteria: the sulfolobus acidocaldarius (
Sulfolobus acidocaldarius), streptomycete (
Streptomyces avermitilis).
In the heterotroph leaching microbacteria: thermophilic acidophilic acid genus bacillus (
Bacillus acidocaldarius), black-koji mould
(Aspergillus), rhodotorula (
Rhodotorula spp.), candiyeast (
Candida), Cryptococcus (
Cryptococcus),
Acontiumcy latium,
Tichosporone crebriae,
Cephalosporium. sP
According to listed each the embodiment data of last table, can produce product of the present invention with the described step of technical scheme part.
Claims (6)
1. one kind is utilized autotrophic type to soak the method that ore deposit bacterium-heterotroph soaks the collaborative subsequent leaching sulphide ores of ore deposit bacterium, in turn includes the following steps:
A, composite mineral-leaching culture of strains: prepare seed culture medium with the growth desired substance element of obligate/facultative autotrophy type leaching microbacteria earlier; 20 ℃～85 ℃ of culture temperature; Incubation time 12h～120h; Medium pH value 0.5～7.0; In 5%～25% ratio inoculation culture obligate autotrophy type or facultative autotrophy type leaching microbacteria, it begins to produce various enzymes, organic acid, metabolite and has when being attended by dead thalline to inoculate obligate heterotroph or facultative heterotroph mikrobe by the time under rotating speed 100rpm～conditions such as 200 rpm, and the bacterial strain with two or more Different Nutrition modes mixes acclimation shaking culture with this understanding;
B, soak the ore deposit culture medium preparation: sulphide ores sample ore break process is the particle about 1mm and is that to use concentration be that rare HCl of 2% carried out immersion treatment 12 hours to it for the ratio of 1:2 according to solid-to-liquid ratio; Then with the sample ore after the immersion treatment with the protection against corrosion ball mill is milled to
and press the solid-to-liquid ratio preparation seed culture medium of v/v (5%～30%) as substrate, regulate medium pH value 0.5～7.0; Wherein the sulphide ores sample ore is one or more in the sulphide oress such as copper glance, purple copper, cubanite, covellite, pyrite, corallite, yellow copper ore, molybdenum glance, pyrrhotite, nickel pyrrhotite, violarite, zink sulphide, nickel sulfide ore, sulfuration manganese ore;
C, composite mineral-leaching bacterial classification soak the ore deposit: will mix in the seed culture medium of efficient composite mineral-leaching inoculation to pretreated breeze as main substrate after the acclimation shaking culture; It is 0.5～7.0 that the ore deposit system pH is soaked in adjusting; 20 ℃～85 ℃ of temperature; Cultivate 24h～240h under the condition of rotating speed 100rpm～200 rpm, carry out the bioleaching reaction of sulphide ores;
Soak in the ore body system at the sulfide mineral that does not have semiconductor property simultaneously, add semi-conductor sulfuration mineral, utilize its semiconductor property, the purpose that in the process of conduction band electron transition, realizes the catalysis microbial leaching is to accelerate to soak the ore deposit reaction;
The extraction of D, metals ion is handled: after reaction; Termination reaction when the metals ion stripping 70% of sulfuration in the breeze is above; Regulate the electrostatic adsorption of eliminating below the pH to 1.8 between ore-leaching bacteria and the mineral with dilute sulphuric acid, behind utilization heavy liquid separation method sedimentation 100min～130min slag and the liquid portion that contains metals ion, mikrobe are separated; Inoculation is soaked mining bacterium and is proceeded to cultivate 12h～240h to accomplish the adsorption and enrichment effect of mikrobe to metals ion in the solution after deriving then; Stop this enrichment reaction when mikrobe in the system reaches 70% to the adsorption and enrichment rate of metals ion when above, add at last microbial flocculant flocculate-centrifugal-dehydration-burning to be to obtain metal oxide mixture.
2. the autotrophic type that utilizes according to claim 1 soaks the method that ore deposit bacterium-heterotroph soaks the collaborative subsequent leaching sulphide ores of ore deposit bacterium; It is characterized in that the sulphide ores kind that this technology is fit to can be one or more in copper glance, purple copper, cubanite, covellite, pyrite, corallite, yellow copper ore, yellow copper ore, molybdenum glance, nickel pyrrhotite, violarite, zink sulphide, nickel sulfide ore, the sulfuration manganese ore.
3. the autotrophic type that utilizes according to claim 1 soaks the method that ore deposit bacterium-heterotroph soaks the collaborative subsequent leaching sulphide ores of ore deposit bacterium; It is characterized in that soaking to have obligate/facultative autotrophy type leaching microbacteria and obligate heterotroph leaching microbacteria simultaneously in the mining composite mineral-leaching bacterial classification.
4. the autotrophic type that utilizes according to claim 1 soaks the method that ore deposit bacterium-heterotroph soaks the collaborative subsequent leaching sulphide ores of ore deposit bacterium, it is characterized in that, all types of composite mineral-leaching mikrobes to soak ore deposit pH value scope be 0.5～7.0.
5. the autotrophic type that utilizes according to claim 1 soaks the method that ore deposit bacterium-heterotroph soaks the collaborative subsequent leaching sulphide ores of ore deposit bacterium; It is characterized in that; The ore deposit pH value scope of soaking of all types of composite mineral-leaching mikrobes is 0.5～7.0 said, and the reagent of regulating the pH value can be dilute sulphuric acid, acetic acid and sodium hydrogencarbonate, yellow soda ash, ammoniacal liquor, calcium hydroxide.
6. the autotrophic type that utilizes according to claim 1 soaks the method that ore deposit bacterium-heterotroph soaks the collaborative subsequent leaching sulphide ores of ore deposit bacterium; It is characterized in that soaking the semi-conductor sulfuration mineral that add in the ore body system at the sulfide mineral that does not have semiconductor property is in copper glance, purple copper, pyrite, pyrrhotite, yellow copper ore, zink sulphide, the sulfuration manganese ore one or more; Adding the breeze size range is
; The semi-conductor sulfuration breeze that adds is to soak 8%～15% of non-semiconductor sulphide ores total amount in the ore body system.
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