CN105969695B - A kind of leaching mine compound bacteria FIM-S1 and its application in high-grade copper-sulphide ores leaching mine - Google Patents
A kind of leaching mine compound bacteria FIM-S1 and its application in high-grade copper-sulphide ores leaching mine Download PDFInfo
- Publication number
- CN105969695B CN105969695B CN201610457540.1A CN201610457540A CN105969695B CN 105969695 B CN105969695 B CN 105969695B CN 201610457540 A CN201610457540 A CN 201610457540A CN 105969695 B CN105969695 B CN 105969695B
- Authority
- CN
- China
- Prior art keywords
- fim
- leaching
- compound bacteria
- leaching mine
- mine compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
- C22B15/00—Obtaining copper
- C22B15/0063—Hydrometallurgy
- C22B15/0065—Leaching or slurrying
-
- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
- C22B3/00—Extraction of metal compounds from ores or concentrates by wet processes
- C22B3/18—Extraction of metal compounds from ores or concentrates by wet processes with the aid of microorganisms or enzymes, e.g. bacteria or algae
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/20—Recycling
Landscapes
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Mechanical Engineering (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Metallurgy (AREA)
- Wood Science & Technology (AREA)
- Manufacturing & Machinery (AREA)
- Materials Engineering (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Geology (AREA)
- Geochemistry & Mineralogy (AREA)
- Environmental & Geological Engineering (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Manufacture And Refinement Of Metals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of leaching mine compound bacteria FIM-S1 and its applications in high-grade copper-sulphide ores leaching mine.By Acidithiobacillus ferrooxidans strain GF (Acidithiobacillus ferrooxidans) FIM-20150107001, pseudomonad (Pseudomonas sp.) FIM-20150107002, thallus 80~90:10 in mass ratio~15:5~8:1~2 of Aminivibrio sp.FIM-20150107003 and enterococcus (Enterococcus sp.) FIM-20150107004 are uniformly mixed, leaching mine compound bacteria is obtained, then obtains leaching mine compound bacteria FIM-S1 after the domestication of copper sulfide sample ore.Leaching mine compound bacteria FIM-S1 of the invention is by autotrophic type and heterotroph microorganism group at these microorganisms are by synergistic effect, and significant to improve Leach reaction dynamics, quickening reaction speed shortens leaching cycle, improves target mine Bioleaching rate and leaching rate.
Description
Technical field
The invention belongs to microbe application fields, and in particular to a kind of leaching mine compound bacteria FIM-S1 and its vulcanize in high-grade
Copper mine soaks the application in mine.
Background technique:
Compared with conventional metallurgical technology, biological metallurgy has the advantages that at low cost, simple process, environmentally friendly, and energy
Enough handle traditional metallurgical method can not or reluctant ore, raw ore and tailing etc..Highly effective ore leaching strain is mineral biology
The bottleneck of extractive technique development finds, acquires and screen to adapt to the efficiency of different ore type and raising bacterial leaching
It is the important research direction of biological metallurgy to ore-leaching bacteria efficient, adaptable, that selectivity is good.
Microorganism currently used for soaking mine is mainly acidophilus, aerobic autotrophic type Thiobacillus, wherein Thiobacillus ferrooxidans
(Acidithiobacillus ferrooxidans) is most study and be most important in current Bioleaching correlative study
A kind of leaching microbacteria, At.ferrooxidans obtain the energy and fixed CO with ferrous oxide and (or) reduced form sulfide2Make
Chemoautotrophic growth is sought for carbon source.Thiobacillus thioxidans is obligate autotrophy microorganism, can oxidation of elemental sulfur, thiosulfate and chain
Four sulfate.In the bioleaching process of sulfide ore, Thiobacillus thioxidans lays under tribute, and generally accompanys and aoxidizes Asia
Iron Thiobacillus plays a role jointly, can significantly accelerated leaching process.In leaching mine environment, microorganism can not be deposited with single culture
It is being carried out by soaking the microbiologic population in ore body system to natural acidic mineral water and ore and industrial bio the study found that leaching
Mine bacterium structure of community has surprising diversity, this application for just soaking mine for Mixed Microbes provides theories integration, also promotes
Development and application research to mixing ore leaching bacteria, the synergistic effect of leaching microbacteria increasingly cause people's in biological metallurgy
Note that can make full use of nutriment since hybrid bacterial strain has mutual supplement with each other's advantages effect, thus mixed cell soaks mine oneself becomes
One of hot spot studied now.
It was found that not only the synergistic effect of the autotrophic types microorganism such as sulfur-oxidizing bacteria and iron-oxidizing bacterium is so that mineral leaching efficiency obtains
To very big raising, and heterotroph microorganism such as acidophilic heterotrophic bacteria (Acidiphilium acidophilum) and sphingol list
The effect in bioleaching system such as born of the same parents bacterium (Sphingomonas sp.) also possesses some special knowledge.Aph.Acidophilum be with
Heterotrophism acidophilic microorganism of the organic matter as the energy, it is previous a large number of studies show that, At.Ferrooxidans and
Both microorganisms of Aph.Acidophilum aoxidize in iron to be made with reduction, utilization of carbon source and mutual releasing growth and metabolic inhibition
With etc. there are apparent conspiracy relations.In bioleaching process, At.ferrooxidans and Aph.acidophilum
Equal acidophilic microorganisms are usually in metal ion (wherein further including some heavy metal ion) environment of high concentration, in order to adapt to
This environment-stress, these microorganisms show to generate more than pure culture some specific metal ions by synergistic effect
High resistance, to be conducive to the raising of its growth and mineral leaching efficiency.Currently with autotrophic type and the collaboration leaching of aerobic ore leaching bacteria
The research of mine is very few.
Summary of the invention:
The first purpose of the invention is to provide a kind of leaching mine compound bacterias.
Leaching mine compound bacteria of the invention, which is characterized in that it is by Acidithiobacillus ferrooxidans strain GF
(Acidithiobacillus ferrooxidans) FIM-20150107001, pseudomonad (Pseudomonas sp.) FIM-
20150107002, Aminivibrio sp.FIM-20150107003 and enterococcus (Enterococcus sp.) FIM-
20150107004 thallus, 80~90:10 in mass ratio~15:5~8:1~2 are uniformly mixed, and obtain leaching mine compound bacteria.
A second object of the present invention is to provide a kind of leaching mine compound bacteria FIM-S1, which is characterized in that it is by above-mentioned leaching
Mine compound bacteria obtains leaching mine compound bacteria FIM-S1 after the domestication of copper sulfide sample ore.
Above-mentioned leaching mine compound bacteria is preferably inoculated into 9K culture medium and cultivates by the domestication, reduces 9K training by generation
The content of ferrous sulfate in base is supported, while increasing the content of copper sulfide sample ore, during the domestication of last generation in culture medium no longer
Ferrous sulfate is added, copper sulfide sample ore becomes unique energy substance, and obtained thallus is again through no ferrous sulfate, with copper-sulphide ores
Sample carries out multiple subculture as the 9K culture medium of unique energy source substance, and gained thallus is to soak mine compound bacteria FIM-S1.
Third object of the present invention is to provide above-mentioned leaching mine compound bacteria FIM-S1 to leach the application in copper in copper-sulphide ores.
The present invention provides a kind of autotrophic types and heterotroph composite mineral-leaching bacterium-leaching efficient, adaptable, selectivity is good
Mine compound bacteria FIM-S1.Compared with certain a kind of leaching microbacteria is used alone, leaching mine compound bacteria FIM-S1 of the invention is by autotrophy
Type and heterotroph microorganism group are at these microorganisms are significant to improve Leach reaction dynamics, quickening reaction by synergistic effect
Speed shortens leaching cycle, improves target mine Bioleaching rate and leaching rate.Leaching mine compound bacteria FIM-S1 of the invention is to height
Ion concentration has certain tolerance, is resistant to the Cu of 30g/L+Concentration, therefore leaching mine compound bacteria FIM-S1 is resistant to
The metal ion of Bioleaching later period high concentration, and it is unlikely to dead.Leaching mine compound bacteria FIM-S1 is a compatible entirety,
Population Ecological keeps relative stability during long-term domestication, the ability with stronger ferrous oxide.
Acidithiobacillus ferrooxidans strain GF (Acidithiobacillus ferrooxidans) FIM- of the invention
20150107001, pseudomonad (Pseudomonas sp.) FIM-20150107002, Aminivibrio sp.FIM-
20150107003 and enterococcus (Enterococcus sp.) FIM-20150107004 belongs to bacterial strain in the prior art, it
From Fujian Microorganism Inst., be preserved in Fujian Microorganism Inst., FIM-20150107001, FIM-
20150107002, FIM-20150107003 and FIM-20150107004 is their deposit number.It is micro- that these are preserved in Fujian Province
Biological study bacterial strain be it is for sale, these bacterial strains be also it is for sale, it is in the prior art before the applying date
Anyone can buy these bacterial strains.And above-mentioned bacterial strains had been proposed on webpage before the applying date (http: //
Www.fjim.org.cn/kytd/ktz/getNews.jsp? newsid=220), bacterial strain the applicant also holds, and guarantees
It is provided in 20 years to the public from the applying date.
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
The preparation of the leaching of embodiment 1 mine compound bacteria FIM-S1
(1) preparation of autotrophic bacterium
With a small amount of Acidithiobacillus ferrooxidans strain GF of transfer needle picking (Acidithiobacillus ferrooxidans,
A.f) FIM-20150107001 thallus accesses in 9K culture medium, and 32 DEG C, 150rpm shake culture deepens red, mirror to culture solution
After inspection observation is with the presence of a large amount of movement bacteriums, bacterium solution is taken, 8000r/min is centrifuged 10min, and incline supernatant, collects thallus.
9K culture medium: A liquid (g/L): (NH4)2SO4 3,K2HPO40.5, KCl 0.1, MgSO4·7H2O 0.5,Ca
(NO3), 0.01, deionized water 800mL, H2SO4Adjust pH to 2.0,121 DEG C of high pressure sterilization 20min；B liquid: FeSO4·7H2O
44.78g/L, deionized water 200mL, H2SO4Adjust pH to 2.0, the membrane filtration degerming that aperture is 0.22 μm, by the A after sterilizing
Liquid dispenses after mixing with B liquid.
(2) preparation of heterotroph
A, inclined-plane culture:
By pseudomonad (Pseudomonas sp.) FIM-20150107002, Aminivibrio sp.FIM-
20150107003 and enterococcus (Enterococcus sp.) FIM-20150107004 is seeded to nutrient agar respectively
On, in 28 DEG C of 2~3d of incubator culture.
B, fermented and cultured:
Pseudomonad (Pseudomonas sp.) FIM- on a small amount of slant medium is provoked with oese
20150107002, Aminivibrio sp.FIM-20150107003 and enterococcus (Enterococcus sp.) FIM-
20150107004 thallus access in fermentation medium, in 2~3d of shaken cultivation on 28 DEG C, the shaking table of 150r/min.Bacterium solution is taken,
8000r/min is centrifuged 10min, and incline supernatant, collects thallus.
Fermentative medium formula (g/L): tryptone 10.0, yeast powder 5.0, sodium chloride 10.0, surplus are water, and pH is certainly
So, after mixing by above-mentioned composition, it sterilizes spare.
Nutrient agar: peptone 10g, beef extract 3g, sodium chloride 5g, agar 15g, distilled water 1000mL, 121 DEG C
High pressure sterilization 15min.
By the Acidithiobacillus ferrooxidans strain GF (Acidithiobacillus ferrooxidans, A.f) of above-mentioned preparation
FIM-20150107001, pseudomonad (Pseudomonas sp.) FIM-20150107002, Aminivibrio sp.FIM-
The thallus 85:12:6:1.5 in mass ratio of 20150107003 and enterococcus (Enterococcus sp.) FIM-20150107004
It is uniformly mixed, obtains leaching mine compound bacteria.
Embodiment 2 soaks the sample ore domestication of mine compound bacteria
Ore domestication culture is carried out to leaching mine compound bacteria prepared by embodiment 1, the culture medium of Initial culture is 9K culture medium,
30 DEG C, 150rpm shaken cultivation in domestication 6 generations of culture, the content of ferrous sulfate in above-mentioned 9K culture medium is reduced by generation, is increased simultaneously
(Zambia's high-grade copper-sulphide ores are sample ore, the granularity ﹤ 0.075mm of sample ore, essential mineral composition to high-grade copper-sulphide ores sample ore
For chalcopyrite and pyrite, copper grade 9.51%) content as energy substance, thus improve leaching mine compound bacteria to target mine
Adaptability.Each shaking flask incubation time is 7~10d, and microscopy is it is observed that largely movement bacterium exists.Last time is tamed
Ferrous sulfate is no longer added in the process, and copper-sulphide ores sample ore becomes unique energy substance.Finally in 9K culture medium (no Fe)
High-grade copper-sulphide ores sample ore is added as unique energy source substance, 3~5 subcultures are carried out to leaching mine compound bacteria, thus to obtain
The stable composite mineral-leaching bacterium tamed by ore, collecting thallus is to soak mine compound bacteria FIM-S1.
The leaching test of 3 high-grade copper-sulphide ores of embodiment
Using Zambia's high-grade copper-sulphide ores as sample ore, the granularity ﹤ 0.075mm of sample ore, essential mineral composition is chalcopyrite
And pyrite, copper grade 9.51%.The leaching mine compound bacteria FIM-S1 obtained in embodiment 2 is accessed in 9K culture medium, 32 DEG C,
150rpm culture, microscopy observation and cell count discovery, leaching mine compound bacteria FIM-S1 pass through of short duration lag phase, just quickly enter
Logarithmic growth phase.Bacterium solution is centrifuged 20min at 8000r/min, supernatant is abandoned and is precipitated, it is sterilized in advance with pH 2.0
Sulfuric acid solution washing precipitating, is then centrifuged for reject supernatant, operates repeatedly three times, to reach Fe in bacteria-removing liquid3+Mesh
, iron-free cell suspension is obtained, is counted under biomicroscope, adjustment cell concentration is 1 × 109cell·mL-1.Take 9K
Culture medium (is free of Fe2+) 180mL is placed in 500mL conical flask, weighs 20g sample ore and be put into conical flask, sulfuric acid adjusts pH values of pulp
Value controls initial pH value 1.8~2.0.It is 1 × 10 that cell concentration, which is added,9cell·mL-1Iron-free cell suspension (mass ratio
5% inoculum concentration), 32 DEG C, 150rpm shake culture.Simultaneously to add Acidithiobacillus ferrooxidans strain GF (Acidithiobacillus
Ferrooxidans, A.f) FIM-20150107001, Acidithiobacillus ferrooxidans strain GF (Acidithiobacillus
Ferrooxidans, A.f) FIM-20150107001 and pseudomonad (Pseudomonas sp.) FIM-20150107002
Mixed liquor is control group according to same method identical additive amount processing, not add ore leaching bacteria as blank control (CK),
Each sample does three parallel control experiments.Acid consumption in leaching process is supplemented by the sulfuric acid of 10mol/L, the moisture of evaporation loss
Pass through addition distilled water supplement.The Cu in leachate is measured after Leach reaction 20d2+Concentration.Blank control group Cu2+Leaching rate is
11%, add the Cu of Acidithiobacillus ferrooxidans strain GF group2+Leaching rate is 43%, addition Acidithiobacillus ferrooxidans strain GF and false unit cell
The Cu of bacterium group2+Leaching rate is 52%, using leaching mine compound bacteria FIM-S1, Cu2+Leaching rate is 69%, shows to rely solely on acidleach
Effect, Cu2+Leaching effect is weaker.Compared with adding Acidithiobacillus ferrooxidans strain GF group (43%), acidophilus ferrous oxide is added
The Cu of Thiobacillus and pseudomonad group2+Leaching rate (52%) is slightly higher.And add the Cu of this leaching mine compound bacteria FIM-S1 group2+It leaches
The enhancing of rate (69%) significant effect.Show that microorganism by synergistic effect, significantly improves leaching rate.
Embodiment 4:
The present embodiment is same as Example 1, and only it is by Acidithiobacillus ferrooxidans strain GF (Acidithiobacillus
Ferrooxidans, A.f) FIM-20150107001, pseudomonad (Pseudomonas sp.) FIM-20150107002,
The thallus of Aminivibrio sp.FIM-20150107003 and enterococcus (Enterococcus sp.) FIM-20150107004
80:15:5:2 in mass ratio is uniformly mixed, and obtains leaching mine compound bacteria A.
By Acidithiobacillus ferrooxidans strain GF (Acidithiobacillus ferrooxidans, A.f) FIM-
20150107001, pseudomonad (Pseudomonas sp.) FIM-20150107002, Aminivibrio sp.FIM-
The thallus 90:10:8:1 in mass ratio of 20150107003 and enterococcus (Enterococcus sp.) FIM-20150107004 is mixed
It closes uniformly, obtains leaching mine compound bacteria B.
It tames leaching mine compound bacteria A and leaching mine compound bacteria B to obtain leaching mine compound bacteria FIM- according to the method for embodiment 2 respectively
S1 carries out the leaching test of high-grade copper-sulphide ores, experimental result discovery, with addition acidophilus oxygen according still further to the method for embodiment 3
Change ferrous Thiobacillus group and addition Acidithiobacillus ferrooxidans strain GF is compared with pseudomonad group, addition leaching mine compound bacteria FIM-S1
The Cu of group2+The enhancing of leaching rate significant effect.Show that microorganism by synergistic effect, significantly improves leaching rate.
Claims (3)
1. a kind of leaching mine compound bacteria, which is characterized in that it is by Acidithiobacillus ferrooxidans strain GF (Acidithiobacillus
Ferrooxidans) FIM-20150107001, pseudomonad (Pseudomonas sp.) FIM-20150107002,
The thallus of Aminivibrio sp.FIM-20150107003 and enterococcus (Enterococcus sp.) FIM-20150107004
80~90:10 in mass ratio~15:5~8:1~2 are uniformly mixed, and obtain leaching mine compound bacteria.
2. a kind of leaching mine compound bacteria FIM-S1, which is characterized in that it is by leaching mine compound bacteria described in claim 1 through copper sulfide
Leaching mine compound bacteria FIM-S1 is obtained after sample ore domestication；The domestication is to be inoculated into leaching mine compound bacteria described in claim 1
It is cultivated in 9K culture medium, the content of ferrous sulfate in 9K culture medium is reduced by generation, while increasing the content of copper sulfide sample ore,
Ferrous sulfate is no longer added during the domestication of last generation in culture medium, copper sulfide sample ore becomes unique energy substance, obtains
Thallus again through no ferrous sulfate, carry out multiple subculture using copper sulfide sample ore as the 9K culture medium of unique energy source substance,
Gained thallus is to soak mine compound bacteria FIM-S1.
3. leaching mine compound bacteria FIM-S1 as claimed in claim 2 leaches the application in copper in copper-sulphide ores.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610457540.1A CN105969695B (en) | 2016-06-22 | 2016-06-22 | A kind of leaching mine compound bacteria FIM-S1 and its application in high-grade copper-sulphide ores leaching mine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610457540.1A CN105969695B (en) | 2016-06-22 | 2016-06-22 | A kind of leaching mine compound bacteria FIM-S1 and its application in high-grade copper-sulphide ores leaching mine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105969695A CN105969695A (en) | 2016-09-28 |
| CN105969695B true CN105969695B (en) | 2019-05-07 |
Family
ID=57021723
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610457540.1A Active CN105969695B (en) | 2016-06-22 | 2016-06-22 | A kind of leaching mine compound bacteria FIM-S1 and its application in high-grade copper-sulphide ores leaching mine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105969695B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114632806B (en) * | 2022-03-22 | 2023-03-24 | 东北大学 | Comprehensive utilization method of high-silicon type iron tailings |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329957A (en) * | 2011-09-09 | 2012-01-25 | 西南科技大学 | Method for continuously leaching sulfide ore by using synergy of autotrophic ore leaching bacteria and heterotrophic ore leaching bacteria |
| CN104862250A (en) * | 2015-05-08 | 2015-08-26 | 周洪波 | Acidophilic iron-oxidizing composite microbial agent and preparation method thereof |
| CN104877933A (en) * | 2015-05-08 | 2015-09-02 | 周洪波 | Bacterial strains capable of being used for preparing acidophilic iron oxidizing microbial compound agent and application thereof |
| CN105199990A (en) * | 2015-10-16 | 2015-12-30 | 福建省微生物研究所 | Autotrophic and heterotrophic compound ore-leaching flora FIM-Z4 and application thereof |
-
2016
- 2016-06-22 CN CN201610457540.1A patent/CN105969695B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102329957A (en) * | 2011-09-09 | 2012-01-25 | 西南科技大学 | Method for continuously leaching sulfide ore by using synergy of autotrophic ore leaching bacteria and heterotrophic ore leaching bacteria |
| CN104862250A (en) * | 2015-05-08 | 2015-08-26 | 周洪波 | Acidophilic iron-oxidizing composite microbial agent and preparation method thereof |
| CN104877933A (en) * | 2015-05-08 | 2015-09-02 | 周洪波 | Bacterial strains capable of being used for preparing acidophilic iron oxidizing microbial compound agent and application thereof |
| CN105199990A (en) * | 2015-10-16 | 2015-12-30 | 福建省微生物研究所 | Autotrophic and heterotrophic compound ore-leaching flora FIM-Z4 and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105969695A (en) | 2016-09-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Enhanced bioleaching efficiency of metals from E-wastes driven by biochar | |
| CN104862250B (en) | Acidophilic iron-oxidizing composite microbial agent and preparation method thereof | |
| CN105861823B (en) | A kind of method for strengthening chalcopyrite Microorganism Leaching | |
| CN102174425B (en) | Acidithiobacillus and application thereof | |
| CN104877933B (en) | Can be used for preparation and have a liking for bacterial strain and the application thereof of sour iron oxidizing microorganisms composite bacteria agent capable | |
| CN103013847B (en) | Ammonia-producing mineral leaching bacterium as well as culture method and application of ammonia-producing mineral leaching bacterium | |
| CN106867921A (en) | The method that thiobacillus ferrooxidans and bacterium treatment acid wastewater in mine reclaim iron resource | |
| CN109967519A (en) | A kind of repairing method of microorganism of Tailings Dam heavy metal pollution | |
| CN109628357A (en) | A kind of ferrous oxide complex microbial inoculum and its application | |
| CN103131650B (en) | Eosino-thiobacillus and application thereof to copper pyrites leaching | |
| CN106906173A (en) | A kind of Thiobacillus thioxidans and its application in removing heavy metals are removed | |
| CN108485976B (en) | Screening method of heavy metal resistant microorganisms | |
| CN102604870A (en) | Recovery culturable viable but non culturable (VBNC) arthrobacterium DSC4 strain and recovering method and application thereof | |
| US20240060153A1 (en) | Acidomyces Acidothermus and Its Application in Leaching Copper-containing Pollutants from Waste Circuit Boards | |
| Gu et al. | Degradation of inhibitory substances by heterotrophic microorganisms during bioleaching of heavy metals from anaerobically digested sewage sludge | |
| CN105969695B (en) | A kind of leaching mine compound bacteria FIM-S1 and its application in high-grade copper-sulphide ores leaching mine | |
| CN103667131B (en) | A kind of method and special strain therefore thereof improving metallic ore leaching rate | |
| CN105733992A (en) | Low-cost high-density culture method of iron sulfur oxidizing bacteria | |
| CN102162029B (en) | Microbiological oxidation and reduction coupling leaching method for valuable metal in manganese oxide ore | |
| CN105199990B (en) | A kind of autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 and its application | |
| CN104531990A (en) | Biological gold extraction process of antimony-bearing complex refractory gold ore and microorganism used in same | |
| CN109439586A (en) | A kind of acidophilus iron oxidizing microorganisms, microbial inoculum and application thereof | |
| CN109554309B (en) | Comamonas W2 and application thereof in denitrification | |
| CN103540519B (en) | Double-layer flat plate and preparation method thereof | |
| CN105400718B (en) | One plant of nitrate relies on ferrous oxidation bacterial strain and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |