CN109628357A - A kind of ferrous oxide complex microbial inoculum and its application - Google Patents
A kind of ferrous oxide complex microbial inoculum and its application Download PDFInfo
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- CN109628357A CN109628357A CN201910111827.2A CN201910111827A CN109628357A CN 109628357 A CN109628357 A CN 109628357A CN 201910111827 A CN201910111827 A CN 201910111827A CN 109628357 A CN109628357 A CN 109628357A
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Abstract
The present invention relates to a kind of ferrous oxide complex microbial inoculum and its applications.Composite bacteria agent includes Thiobacillus ferrooxidans microorganism and ferrous oxide hook end spiral bacteria microorganism.Complex microbial inoculum of the present invention has the advantages that environmental-friendly, tolerance is strong, and adaptability is high.Complex microbial inoculum of the present invention can be normal growth in the 9K culture medium of 35g/L in circuit board concentration.Therefore the metal that complex microbial inoculum of the present invention is suitable for circuit board under different condition leaches.It utilizes, recycle for realization board metal resource secondary, effective protection mining area ecological environment is contributed; reach simultaneously and obtains economic benefit and environmental-friendly win-win situation; and complex microbial inoculum green non-pollution of the present invention; it less will cause secondary pollution after metal in leaching circuit board; therefore the present invention is ideal efficient complex microbial inoculum in terms of biological metallurgy, can be widely applied to biological metallurgy field.
Description
Technical field
The invention belongs to biological metallurgy fields, and in particular to a kind of ferrous oxide complex microbial inoculum and its leach circuit
The purposes of sheetmetal.
Background technique
Currently, recycling metal from electron wastes both at home and abroad mainly has 4 kinds of processing techniques, i.e. mechanical treatment technology, heat
Processing technique, hydrometallurgical technology and biotechnology, these technologies achieve certain effect, but preceding 3 kinds of sides to a certain extent
Method is generally lower to metal recovery rate, can not be completely separated metal, easily causes secondary pollution to environment.And biosorption process has
The noble metal in electron wastes is recycled since its is high-efficient, at low cost, energy consumption is small, many merits such as secondary pollution are not generated,
Become one of the technology of most prospect.In the circuit board containing precious metals such as copper, iron, nickel, tin, zinc, gold, silver, palladiums.With
For copper metal, the most abundant chalcopyrite of reserves is usually relatively low, the high cost that conventional high temperature is smelted, it is difficult to realize its economic valence
Value.However its complicated component, crystal structure are fine and close, bioleaching process there is also leaching cycles it is long, efficiency is relatively low the problems such as,
Therefore, the metals such as copper are recycled from circuit board, realize that the secondary use of metal is imperative.Bioleaching is microorganism and metal
The biochemical process to react to each other, major function microorganism are acidophilus iron-oxidizing bacteria.In the leaching of circuit board, the oxidation of acidophilus iron
Ferrous iron in the oxidable Leaching Systems of bacterium generates iron ion, as oxidant attack circuit plate, discharge ferrous ion and copper from
Son.Therefore, the growth of the leaching process quasi-microorganism is extremely important.There are general character disadvantages for strain used in Bioleaching at present: leaching
Timeliness is short out.Over time, leaching efficiency slowly declines, and finally stops, generally low efficiency.And since circuit board soaks
Process out, the continuous fluctuation of chemical parameters, so that the also continuous succession in entire leaching process of the ratio of strain.How preferably
The microorganism ratio in the iron-oxidizing bacteria kind of different phase is balanced, so that it is played maximum efficiency in leaching process, then needs root
According to the growth of leaching process microorganism and the variation of chemical parameters, the leaching of such functional microorganism is regulated and controled by external source means intervention
Process out.(patent No. CN201711148633.7) such as Ruan Jujun proposes a kind of two steps of valuable metal in total head recovery train plate
Bioleaching process and device.But its device is more harsh to environmental requirement, and technique is cumbersome.Therefore, it studies a kind of easy
The complex microbial inoculum of acquisition is used to leach the great meaning of metal in retrieving circuit board, invent a kind of high timeliness, low consumption,
It is imperative easily to obtain complex microbial inoculum.
For this purpose, the method for leaching metal based on microorganism growth and chemical regulation enhancing circuit board is further explored, from mentioning
Set about in terms of the microorganism efficiency for rising iron-oxidizing bacteria, growth and the iron metabolism for improving the microorganism of bioleaching process are active
Degree, the final circuit board leaching efficiency that promoted have very important significance.
Summary of the invention
In view of the problems of prior art, the present invention provides a kind of ferrous oxide complex microbial inoculum and its answers
With.The bioremediation that different metal in circuit board is leached is suitable for by two kinds of leaching microbacteria immixtures
Metal in circuit board leaches.Ecological environment can be effectively protected, efficiently utilize resource.Microbial bacterial agent of the present invention has
It is lasting conducive to metal resource recycling, environmental protection, leaching effect, leaching efficiency height high to circuit board tolerance rate, microorganism
The advantages that microbial inoculum stability is strong.
The technical scheme to solve the above technical problems is that
A kind of ferrous oxide complex microbial inoculum, including Thiobacillus ferrooxidans microorganism and ferrous oxide hook end spiral
Bacteria microorganism.
The beneficial effects of the present invention are: the present invention is by two kinds of leaching microbacteria immixtures, it is golden to difference in circuit board
Belong to the bioremediation leached, the metal suitable for circuit board leaches.Ecological environment can be effectively protected, efficiently
Utilize resource.Microbial bacterial agent of the present invention be conducive to metal resource recycling, environmental protection, leach effect it is lasting, it is right
The advantages that circuit board tolerance rate is high, leaching efficiency is high, and microbial bacterial agent stability is strong.
Based on the above technical solution, the present invention can also be improved as follows.
Further, the Thiobacillus ferrooxidans microorganism is selected from the following are a kind of or several in the microorganism of 44 kinds of deposit numbers
Kind；The microorganism of 44 kinds of deposit numbers be respectively as follows: CCTCCAB206199, CCTCCAB206200, CCTCCAB206201,
CCTCCAB206202、CCTCCAB206203、CCTCCAB206204、CCTCCAB206205、CCTCCAB206206、
CCTCCAB206207、CCTCCAB206208、CCTCCAB207053、CCTCCAB207054、CCTCCAB207055、
CCTCCAB207056,CCTCCAB207057,CCTCCAB207058；ATCC13598D,ATCC53987,ATCC23270,
ATCC53982、ATCC53983、ATCC14119、ATCC19859、ATCC33020、ATCC21834、ATCC13728、
ATCC13661；DSM-2390,DSM-1931,DSM-14882,DSM-1928,DSM-1929,DSM-2389,DSM-700,DSM-
2613、DSM-29444、DSM-9464、DSM-11477、DSM-24419、DSM-9465、DSM-1927、DSM-584、DSM-
583、DSM-585。
Beneficial effect using the above scheme is: being conducive to further increase leaching effect using the microorganism of above-mentioned deposit number
Power, to circuit board tolerance rate, leaching efficiency is high and microbial bacterial agent stability.
Further, a kind of in microorganism of the ferrous oxide hook end spiral bacteria microorganism selected from following 20 kinds of deposit numbers or
It is several；The microorganism of 20 kinds of deposit numbers be respectively as follows: CCTCCAB206158, CCTCCAB206159, CCTCCAB206160,
CCTCCAB206161、CCTCCAB206162、CCTCCAB206163、CCTCCAB206164、CCTCCAB207036、
CCTCCAB207037,CCTCCAB207038；ATCC53992,ATCC29047,ATCC49879,ATCC49880,
ATCC53993；DSM-2391,DSM-2705,DSM-1937,DSM-1929,DSM-1928.
Beneficial effect using the above scheme is: being conducive to further increase leaching effect using the microorganism of above-mentioned deposit number
Power, to circuit board tolerance rate, leaching efficiency is high and microbial bacterial agent stability.
Further, the thalline quantity ratio of Thiobacillus ferrooxidans microorganism and ferrous oxide hook end spiral bacteria microorganism is
(1-3):(3-1)。
Beneficial effect using the above scheme is: being conducive to further increase using above-mentioned ratio and leaches effect, to electricity
Road plate tolerance rate, leaching efficiency height and microbial bacterial agent stability.
Further, the thalline quantity ratio of Thiobacillus ferrooxidans microorganism and ferrous oxide hook end spiral bacteria microorganism is
1:1 or 1:2 or 1:3 or 2:1 or 3:1.
Beneficial effect using the above scheme is: being conducive to further increase using above-mentioned ratio and leaches effect, to electricity
Road plate tolerance rate, leaching efficiency height and microbial bacterial agent stability.
The present invention provides above-mentioned ferrous oxide complex microbial inoculum and is leaching the application in metal.It is particularly suitable for leaching electricity
Metal in the plate of road.
Beneficial effect using the above scheme is: complex microbial inoculum provided by the invention can metal leaching in into
Row application.It is especially suitable for leaching the metal in circuit board, convenient for recycling and secondary use, solves serious gold caused by circuit board
The problem of belonging to pollution.It can also be further widely applied in environmental protection, such as: during specific implementation, lead to
Peroxidating Fe2+The Fe of acquisition3+Aoxidize H2S exhaust gas, to realize H2S exhaust-gas treatment.Complex microbial inoculum of the present invention can be in ring
The fields such as border protection and board metal leaching are widely applied.Be conducive to metal recovery utilization, environmental protection, metal leach
The advantages that rate is high, high to circuit board tolerance rate.Complex microbial inoculum provided by the invention can be 35g/L in circuit board concentration
Environment in normal growth.
It is including following the present invention also provides a kind of method for leaching metal using above-mentioned ferrous oxide complex microbial inoculum
Step: above-mentioned ferrous oxide complex microbial inoculum being seeded in 9K culture medium, circuit board is added in 9K culture medium, hair
Ferment culture.
Beneficial effect using the above scheme is: being conducive to metal resource recycling benefit using above method Leaching of Gold accessory
With, environmental protection, to leach effect lasting, high to circuit board tolerance rate, leaching efficiency is high, and it is excellent that microbial bacterial agent stability is strong etc.
Point.
Further, when fermented and cultured, cultivation temperature is 35-45 DEG C.
Beneficial effect using the above scheme is: being conducive to the growth of thallus using above-mentioned cultivation temperature, if temperature mistake
It is low to cause thalli growth slow or non-growing problem, cause thalli growth slow or even non-growing problem if temperature is excessively high.
Further, in 9K culture medium, initial Fe2+Concentration is 10-30g/L.
Beneficial effect using the above scheme is: suitable Fe2+Concentration is conducive to the growth of thallus, if Fe2+Concentration mistake
The low problem for being easy to cause thalli growth slow, if Fe2+Concentration is excessively high to be easy to cause the slow problem of thalli growth.
Further, initial pH value of medium 2.0-3.0, time of fermented and cultured are 20-30 days, shaking table when fermented and cultured
Revolving speed is 120-180rpm.
Beneficial effect using the above scheme is: being conducive to the growth of thallus using above-mentioned condition of culture.If pH value
It is too low to be easy to cause thalli growth slow or non-growing problem, it is easy to cause thalli growth slowly to ask if pH value is excessively high
Topic.
It, can be with specifically, using the described Bioleaching recovery method for leaching complex microbial inoculum and carrying out circuit board
The following steps are included:
(1) pretreatment of circuit board
The circuit board that will be used to leach is pre-processed, and removing socket, battery with plastics etc., all are removable
Component, and circuit board is crushed blocking (size is 5mm × 5mm or so).
(2) preparation of complex microbial inoculum
This complex microbial inoculum is micro- by a kind of Thiobacillus ferrooxidans microorganism and a kind of ferrous oxide hook end spirillum
Biotic component, Thiobacillus ferrooxidans microorganism and ferrous oxide hook end spirillum microbial cells quantitative proportion are preferably 1:1
Or 1:2 or 1:3 or 2:1 or 3:1.
(3) board metal leaches
It by circuit board or is added in the 9K culture medium containing complex microbial inoculum, is put into shaking table and is cultivated, cultivate
Condition: pH value 2.0-3.0, cultivation temperature are 35-45 DEG C, and incubation time is 20-30 days, initial Fe2+Concentration is 10-30g/L,
Shaking speed 180rpm.
(4) leaching liquor for obtaining above-mentioned steps is measured, and before being measured, is shaken up, is taken 0.1mL to leach after standing
Liquid is diluted to 60mL with distilled water, determines copper ion, nickel ion with kit (the triumphant microorganism Science and Technology Ltd. in Guangdong)
Concentration.
In circuit board processing method described in step (1), circuit board also can be directly used for leaching in the present invention, but electric
Road plate, which is crushed blocking (size is 5mm × 5mm or so), to be increased with the contact area of culture solution, and extraction time can be greatly decreased,
Improve leaching efficiency.
Complex microbial inoculum described in step (2), in this way the advantages of preparation are: iron oxidation activity with higher,
And the repellency between bacterial strain is avoided, the problem of low efficiency, this complex microbial inoculum can be used as an entirety and use,
Have the advantages that stability is high, simple to strong environmental adaptability, leaching efficiency height, preparation.Effectively low consumption it can leach circuit
Metal in plate, convenient for recycling and secondary use.
In method described in step (3), complex microbial inoculum is in circuit board leaching process with CO2For carbon source, will soak
Fe in liquid out2+It is oxidized to Fe3+, metal ion is converted into free ionic condition by stationary state, can use displacement reaction
METAL EXTRACTION recycling to ionic condition.
Leaching process described in step (1) (2) (3) (4), the concentration that circuit board is added are no more than 35g/L.
Specific Fe can be configured in the following proportions2+The 9K culture medium of concentration, such as prepare Fe2+The 9K of concentration 30g/L is cultivated
Base, formula can be with are as follows: 0.3g (NH4)2SO4, 0.1gKCl, 0.5gK2HPO4, 0.5gMgSO4·7H2O, 0.01gCa (NO3)2,
30gFeSO4·7H2O and 1000mL distilled water, finally uses 0.5mol/LH2SO4PH value is adjusted to 1.5-2.0, obtains 9K training
Support base.
Specific embodiment
The principles and features of the present invention are described below, and the given examples are served only to explain the present invention, is not intended to limit
Determine the scope of the present invention.
Technical solution of the present invention unless otherwise defined, all technical terms used hereinafter and those skilled in the art
It is identical to be generally understood meaning.
Technical term used herein is intended merely to the purpose of description specific embodiment, is not intended to and limits this hair
Bright protection scope.Unless otherwise specified, various raw material, reagent, the instrument and equipment etc. used in the present invention
It is commercially available or can be prepared by existing method by market.It is not limited to be exemplified below specific embodiment, also wrap
Include any combination between specific embodiment.
The present invention provides a kind of ferrous oxide complex microbial inoculum and its leaches the purposes of board metal, it is related to one
Kind ferrous oxide complex microbial inoculum and its purposes for leaching board metal.Bioleaching aspect research and utilization is micro- at present
Biological single strain and compound strain, either single strain or compound strain all have the shortcomings that leaching timeliness is short.From leaching
Journey starts, and over time, the leaching ability of bacterial strain is gradually reduced, and rarely having bacterial strain that can continuously leach 28 days can also
It is kept to leach activity.
And ferrous oxide complex microbial inoculum provided by the present application experiments verify that leach 35 days be still to be able to maintain
70% or more Fe2+Oxygenation efficiency, when leaching 28 days, Fe2+Oxygenation efficiency highest, can achieve 97.78%.
The Thiobacillus ferrooxidans be 44 kinds of deposit numbers be CCTCCAB206199, CCTCCAB206200,
CCTCCAB206201、CCTCCAB206202、CCTCCAB206203、CCTCCAB206204、CCTCCAB206205、
CCTCCAB206206、CCTCCAB206207、CCTCCAB206208、CCTCCAB207053、CCTCCAB207054、
CCTCCAB207055,CCTCCAB207056,CCTCCAB207057,CCTCCAB207058；ATCC13598D,ATCC53987,
ATCC23270、ATCC53982、ATCC53983、ATCC14119、ATCC19859、ATCC33020、ATCC21834、
ATCC13728,ATCC13661；DSM-2390,DSM-1931,DSM-14882,DSM-1928,DSM-1929,DSM-2389,
DSM-700、DSM-2613、DSM-29444、DSM-9464、DSM-11477、DSM-24419、DSM-9465、DSM-1927、
The one or more of DSM-584, DSM-583, DSM-585；The ferrous oxide hook end spirillum is that 20 kinds of deposit numbers are
CCTCCAB206158、CCTCCAB206159、CCTCCAB206160、CCTCCAB206161、CCTCCAB206162、
CCTCCAB206163,CCTCCAB206164,CCTCCAB207036,CCTCCAB207037,CCTCCAB207038；
ATCC53992,ATCC29047,ATCC49879,ATCC49880,ATCC53993；DSM-2391,DSM-2705,DSM-1937,
The one or more of DSM-1929, DSM-1928；
Above-mentioned strain name are as follows:
CCTCCAB206199 Thiobacillus ferrooxidans CSU206059Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB206200 Thiobacillus ferrooxidans CSU206060Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB206201 Thiobacillus ferrooxidans CSU206062Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB206202 Thiobacillus ferrooxidans CSU206064Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB206203 Thiobacillus ferrooxidans CSU206065Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB206204 Thiobacillus ferrooxidans CSU206066Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB206205 Thiobacillus ferrooxidans CSU206068Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB206206 Thiobacillus ferrooxidans CSU206069Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB206207 Thiobacillus ferrooxidans CSU206071Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB206208 Thiobacillus ferrooxidans CSU206073Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB207053 Thiobacillus ferrooxidans CSU206061Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB207054 Thiobacillus ferrooxidans CSU206063Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB207055 Thiobacillus ferrooxidans CSU206067Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB207056 Thiobacillus ferrooxidans CSU206070Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB207057 Thiobacillus ferrooxidans CSU206072Acidithiobacillusferrooxidans (letter
Claim A.f)
CCTCCAB207058 Thiobacillus ferrooxidans CSU206074Acidithiobacillusferrooxidans (letter
Claim A.f)
ATCC13598DAcidithiobacillusferrooxidans(TempleandColmer)
KellyandWoodATCC131598D (abbreviation A.f)
ATCC53987Acidithiobacillusferrooxidans(TempleandColmer)
KellyandWoodATCC53987 (abbreviation A.f)
ATCC23270Acidithiobacillusferrooxidans(TempleandColmer)
KellyandWoodATCC23270 (abbreviation A.f)
ATCC53982Acidithiobacillusferrooxidans(TempleandColmer)
KellyandWoodATCC53982 (abbreviation A.f)
ATCC53983Acidithiobacillusferrooxidans(TempleandColmer)
KellyandWoodATCC53983 (abbreviation A.f)
ATCC14119Acidithiobacillusferrooxidans(TempleandColmer)
KellyandWoodATCC14119 (abbreviation A.f)
ATCC19859Acidithiobacillusferrooxidans(TempleandColmer)
KellyandWoodATCC19859 (abbreviation A.f)
ATCC33020Acidithiobacillusferrooxidans(TempleandColmer)
KellyandWoodATCC33020 (abbreviation A.f)
ATCC21834Acidithiobacillusferrooxidans(TempleandColmer)
KellyandWoodATCC21834 (abbreviation A.f)
ATCC13728Acidithiobacillusferrooxidans(TempleandColmer)
KellyandWoodATCC13728 (abbreviation A.f)
ATCC13661Acidithiobacillusferrooxidans(TempleandColmer)
KellyandWoodATCC13661 (abbreviation A.f)
DSM-2613Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-29444Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-9464Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-11477Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-24419Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-9465Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-1927Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-584Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-583Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-585Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-14882Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-2390Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-1931Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-1928Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-1929Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-2389Acidithiobacillusferrooxidans (abbreviation A.f)
DSM-700Acidithiobacillusferrooxidans (abbreviation A.f)
CCTCCAB206158 ferrous oxide hook end spirillum CSU206002Leptospirillumferrooxidans (letter
Claim L.f)
CCTCCAB206159 ferrous oxide hook end spirillum CSU206003Leptospirillumferrooxidans (letter
Claim L.f)
CCTCCAB206160 ferrous oxide hook end spirillum CSU206004Leptospirillumferrooxidans (letter
Claim L.f)
CCTCCAB206161 ferrous oxide hook end spirillum CSU206005Leptospirillumferrooxidans (letter
Claim L.f)
CCTCCAB206162 ferrous oxide hook end spirillum CSU206006Leptospirillumferrooxidans (letter
Claim L.f)
CCTCCAB206163 ferrous oxide hook end spirillum CSU206008Leptospirillumferrooxidans (letter
Claim L.f)
CCTCCAB206164 ferrous oxide hook end spirillum CSU206009Leptospirillumferrooxidans (letter
Claim L.f)
CCTCCAB207036 ferrous oxide hook end spirillum CSU206001Leptospirillumferrooxidans (letter
Claim L.f)
ATCC53992Leptospirillumferrooxidans(TempleandColmer)
KellyandWoodATCC53992 (abbreviation L.f)
ATCC29047Leptospirillumferrooxidans(TempleandColmer)
KellyandWoodATCC29047 (abbreviation L.f)
ATCC49879Leptospirillumferrooxidans(TempleandColmer)
KellyandWoodATCC49879 (abbreviation L.f)
ATCC49880Leptospirillumferrooxidans(TempleandColmer)
KellyandWoodATCC49880 (abbreviation L.f)
ATCC53993Leptospirillumferrooxidans(TempleandColmer)
KellyandWoodATCC53993 (abbreviation L.f)
DSM-2705Leptospirillumferrooxidans (abbreviation L.f)
DSM-2391Leptospirillumferrooxidans (abbreviation L.f)
DSM-1931Leptospirillumferrooxidans (abbreviation L.f)
DSM-1929Leptospirillumferrooxidans (abbreviation L.f)
DSM-1928Leptospirillumferrooxidans (abbreviation L.f)
Through inventor's experimental verification, under following experiment condition: the initial Fe of culture medium2+Concentration is 20g/L, and pH value is
2.25, fermentation time is 28 days, and cultivation temperature is 35 DEG C, and circuit board concentration is 30g/L, Thiobacillus ferrooxidans microorganism and oxygen
Changing ferrous hook end spirillum microbial cells quantitative proportion is 1:2, and fermentation time is 28 days.Using above-mentioned deposit number bacterial strain into
Row assembles, Fe2+97% or more oxygenation efficiency, the leaching rate of copper are 78%-84%.The leaching rate of nickel is 60%-64%.
The advantages of this complex microbial inoculum, is: manually assembling, utilizes a kind of Thiobacillus ferrooxidans microorganism and one
Kind ferrous oxide hook end spiral bacteria microorganism, both microorganisms are complemented each other, and there is no repelling and competing, stability is good, obtains
It obtains simply, leaching efficiency is high, and leaching timeliness is long, effectively can leach to low consumption the metal in circuit board, therefore can be used for ring
Border protection and metal leach.
According to non-patent literature 1.LiuJin-yan, TaoXiu-xiang, CaiPei, etal.Studyofformation
ofjarositemediatedbythiobacillusferrooxidansin9Kmedium[J].ProcediaEarthandPl
anetaryScience(2009),706–712；And the tall star of non-patent literature 2., Liu Guanlan, Zhou Lixiang wait ferrous oxide
Influence [J] the ACTA Scientiae Circumstantiae of Thiobacillus density and nutrition supply to troilite biological oxidation, 2018, (2) etc. reports table
It is bright, Fe2+Oxygenation efficiency can be used as the index for judging iron-oxidizing bacteria growing state, so the present invention uses Fe2+Oxygenation efficiency is as this
The foundation of complex microbial inoculum growing state.
The strain of above-mentioned deposit number is commercially available bacterial strain.The public can be in China typical culture collection center (CCTCC), beauty
State's type culture collection warehousing (ATCC), Germany Microbiological Culture Collection Center (DSMZ) purchase obtain.
Specific embodiment 1: this complex microbial inoculum wherein contains a kind of Thiobacillus ferrooxidans microorganism and one kind
Ferrous oxide hook end spiral bacteria microorganism, Thiobacillus ferrooxidans microorganism and ferrous oxide hook end spirillum microbial cells
Quantitative proportion is 1:1 or 1:2 or 1:3 or 2:1 or 3:1.
Present embodiment ferrous oxide complex microbial inoculum, which is inoculated in 9K culture medium, to be cultivated, and 9K culture medium is by 0.3g
(NH4)2SO4, 0.1gKCl, 0.5gK2HPO4, 0.5gMgSO4·7H2O, 0.01gCa (NO3)2, 10-30g FeSO4·7H2O,
1000mL distilled water, finally uses 0.5mol/LH2SO4Adjustment pH value is obtained to 2.0-3.0.It will by the inoculum concentration of 10% (v/v)
Ferrous oxide complex microbial inoculum is inoculated in 9K culture medium, in initial Fe2+Concentration is 10-30g/L, cultivation temperature 30-
45 DEG C, shaking speed is cultivated 20-30 days under conditions of being 180rpm.
Experimental result: the appropriate incubation temperature of complex microbial inoculum of the present invention is 30-40 DEG C, and most suitable cultivation temperature is 35
℃。
Specific experiment the results are shown in Table 1 (experiment condition are as follows: the initial Fe of culture medium2+Concentration is 20g/L, pH value 2.25, hair
The ferment time is 28 days, and circuit board concentration is 30g/L, Thiobacillus ferrooxidans microorganism and ferrous oxide hook end spiral bacteria microorganism
Thalline quantity ratio is 1:2, and bacterial strain uses therefor is the Thiobacillus ferrooxidans microorganism and preservation that deposit number is ATCC23270 respectively
Number be ATCC29047 ferrous oxide hook end spiral bacteria microorganism).
Experimental result under 1 condition of different temperatures of table
| Temperature (DEG C) | 30 | 35 | 40 | 45 |
| Fe2+Oxygenation efficiency | 50.23% | 97.78% | 72.42% | 19.84% |
Specific embodiment 2: the difference of present embodiment and specific embodiment one is: cultivation temperature is 35 DEG C,
His step and parameter are identical as embodiment one.
Experimental result: the suitable fermentation time of complex microbial inoculum of the present invention is 20-30 days, and most suitable fermentation time is 28
It.Specific experiment the results are shown in Table 2 (experiment conditions are as follows: the initial Fe of culture medium2+Concentration is 20g/L, pH value 2.25, when fermentation
Between be 28 days, cultivation temperature be 35 DEG C, circuit board concentration be 30g/L, Thiobacillus ferrooxidans microorganism and ferrous oxide hook end
Spirillum microbial cells quantitative proportion is 1:2, and bacterial strain uses therefor is the Thiobacillus ferrooxidans that deposit number is ATCC23270 respectively
The ferrous oxide hook end spiral bacteria microorganism that microorganism and deposit number are ATCC29047).
Experimental result under 2 different fermentations time conditions of table
Specific embodiment 3: the difference of present embodiment and specific embodiment two is: incubation time is 28 days,
His step and parameter are identical as embodiment two.
Experimental result: the proper growth Fe of complex microbial inoculum of the present invention2+Concentration is 10-30g/L, the most suitable growth Fe2+
Concentration is 20g/L.Specific experiment the results are shown in Table 3 (experiment conditions are as follows: the pH value of culture medium is 2.25, and fermentation time is 28 days, training
Supporting temperature is 35 DEG C, and circuit board concentration is 30g/L, Thiobacillus ferrooxidans microorganism and ferrous oxide hook end spiral bacteria microorganism
Thalline quantity ratio is 1:2, and bacterial strain uses therefor is the Thiobacillus ferrooxidans microorganism and preservation that deposit number is ATCC23270 respectively
Number be ATCC29047 ferrous oxide hook end spiral bacteria microorganism).
3 Fe of table2+Experimental result under different condition
| Fe2+Concentration (g/L) | 0 | 10 | 15 | 20 | 30 | 40 |
| Fe2+Oxygenation efficiency | 0% | 65.34% | 76.25% | 97.78% | 80.23% | 69.72% |
Specific embodiment 4: the difference of present embodiment and embodiment three is the initial Fe of 9K culture medium2+Concentration is
20g/L, other parameters are identical as embodiment three.
Experimental result: the suitable fermentation pH value of complex microbial inoculum of the present invention is 2.0-3.0, and most suitable fermentation pH value is
2.25.Specific experiment the results are shown in Table 4 (experimental conditions are as follows: the initial Fe of culture medium2+Concentration is 20g/L, and fermentation time is 28 days,
Cultivation temperature is 35 DEG C, and circuit board concentration is 30g/L, Thiobacillus ferrooxidans microorganism and the micro- life of ferrous oxide hook end spirillum
Object thalline quantity ratio is 1:2, and bacterial strain uses therefor is the Thiobacillus ferrooxidans microorganism and guarantor that deposit number is ATCC23270 respectively
Hiding number is the ferrous oxide hook end spiral bacteria microorganism of ATCC29047).
Experimental result under 4 different pH condition of table
Technical solution of the present invention is introduced below by specific embodiment.
Embodiment 1 configures 9K culture medium: 0.3g (NH into embodiment 6 in the following proportions4)2SO4, 0.1gKCl,
0.5gK2HPO4, 0.5gMgSO4·7H2O, 0.01gCa (NO3)2, 20gFeSO4·7H2O and 1000mL distilled water, is finally used
0.5mol/LH2SO4PH value is adjusted to 2.25, obtains 9K culture medium.
Embodiment 1
Ferrous oxide complex microbial inoculum is by a kind of Thiobacillus ferrooxidans microorganism and a kind of ferrous oxide hook end spiral shell
It revolves bacteria microorganism to form, the thalline quantity ratio of Thiobacillus ferrooxidans microorganism and ferrous oxide hook end spiral bacteria microorganism is 1:
2, bacterial strain uses therefor is the Thiobacillus ferrooxidans microorganism that deposit number is ATCC23270 respectively and deposit number is ATCC29047
Ferrous oxide hook end spiral bacteria microorganism).
Ferrous oxide complex microbial inoculum is inoculated in 9K culture medium by 10% (v/v) inoculum concentration, and is cultivated to 9K
Circuit board is added in base, is 35 DEG C in cultivation temperature, shaking speed 180rpm, initial Fe2+Concentration is 20g/L, and pH value is
Fermented and cultured 100 hours under conditions of 2.25.
Be added circuit board concentration be respectively 1g/L, 5g/L, 10g/L, 15g/L, 20g/L, 25g/L, 30g/L, 35g/L and
45g/L。
As a result, it has been found that complex microbial inoculum of the present invention be added concentration be 35g/L circuit board 9K culture medium in still
So can be with normal growth, but when circuit board concentration is more than 35g/L, this ferrous oxide complex microbial inoculum cannot be grown.
Illustrate the circuit board tolerance rate of this complex microbial inoculum up to 35g/L.
Embodiment 2
Ferrous oxide complex microbial inoculum is by a kind of Thiobacillus ferrooxidans microorganism and a kind of ferrous oxide hook end spiral shell
Bacteria microorganism composition is revolved, bacterial strain uses therefor is the Thiobacillus ferrooxidans microorganism and deposit number that deposit number is ATCC23270 respectively
For the ferrous oxide hook end spiral bacteria microorganism of ATCC29047, contain Thiobacillus ferrooxidans microorganism and ferrous oxide hook end
The thalline quantity ratio of spiral bacteria microorganism is 1:1.
Ferrous oxide complex microbial inoculum is inoculated in 9K culture medium by 10% (v/v) inoculum concentration, and is cultivated to 9K
The circuit board that concentration is 10g/L is added in base, is 35 DEG C in cultivation temperature, shaking speed 180rpm, initial Fe2+Concentration is
20g/L, pH value leach 28 days under conditions of being 2.25, take out conical flask later, and rocking mixes precipitating, stand, until precipitating
After stabilization, 0.1mL leachate is taken to be diluted to 60mL with distilled water, with kit (the triumphant microorganism Science and Technology Ltd. in Guangdong) to leaching
Copper ion, nickel ion in extract are measured.
Measurement result: the leaching rate of copper is 78.31%.The leaching rate of nickel is 60.22%.
Embodiment 3
Ferrous oxide complex microbial inoculum is by a kind of Thiobacillus ferrooxidans microorganism and a kind of ferrous oxide hook end spiral shell
Bacteria microorganism composition is revolved, bacterial strain uses therefor is the Thiobacillus ferrooxidans microorganism and deposit number that deposit number is ATCC23270 respectively
For the ferrous oxide hook end spiral bacteria microorganism of ATCC29047, contain Thiobacillus ferrooxidans microorganism and ferrous oxide hook end
The thalline quantity ratio of spiral bacteria microorganism is 1:2.
Ferrous oxide complex microbial inoculum is inoculated in 9K culture medium by 10% (v/v) inoculum concentration, and is cultivated to 9K
The circuit board that concentration is 10g/L is added in base, is 35 DEG C in cultivation temperature, shaking speed 180rpm, initial Fe2+Concentration is
20g/L, pH value leach 28 days under conditions of being 2.25, take out conical flask later, and rocking mixes precipitating, staticly settles stabilization
Afterwards, 0.1mL leachate is taken to be diluted to 60mL with distilled water, with kit (the triumphant microorganism Science and Technology Ltd. in Guangdong) to leaching liquor
In copper ion, nickel ion is measured.
Measurement result: it is 80.64% that the leaching rate of copper, which reaches,.The leaching rate of nickel is 62.34%.
Embodiment 4
Ferrous oxide complex microbial inoculum is by a kind of Thiobacillus ferrooxidans microorganism and a kind of ferrous oxide hook end spiral shell
Bacteria microorganism composition is revolved, bacterial strain uses therefor is the Thiobacillus ferrooxidans microorganism and deposit number that deposit number is ATCC23270 respectively
For the ferrous oxide hook end spiral bacteria microorganism of ATCC29047, contain Thiobacillus ferrooxidans microorganism and ferrous oxide hook end
The thalline quantity ratio of spiral bacteria microorganism is 1:3.
Ferrous oxide complex microbial inoculum is inoculated in 9K culture medium by 10% (v/v) inoculum concentration, and is cultivated to 9K
The circuit board that concentration is 10g/L is added in base, is 35 DEG C in cultivation temperature, shaking speed 180rpm, initial Fe2+Concentration is
20g/L, pH value leach 28 days under conditions of being 2.25, take out conical flask later, and rocking mixes precipitating, staticly settles stabilization
Afterwards, 0.1mL leachate is taken to be diluted to 60mL with distilled water, with kit (the triumphant microorganism Science and Technology Ltd. in Guangdong) to leaching liquor
In copper ion, nickel ion is measured.
Measurement result: the leaching rate of copper is 83.96%.The leaching rate of nickel is 63.81%.
Embodiment 5
Ferrous oxide complex microbial inoculum is by a kind of Thiobacillus ferrooxidans microorganism and a kind of ferrous oxide hook end spiral shell
Bacteria microorganism composition is revolved, bacterial strain uses therefor is the Thiobacillus ferrooxidans microorganism and deposit number that deposit number is ATCC23270 respectively
For the ferrous oxide hook end spiral bacteria microorganism of ATCC29047, Thiobacillus ferrooxidans microorganism and ferrous oxide hook end spiral
The thalline quantity ratio of bacteria microorganism is 2:1.
Ferrous oxide complex microbial inoculum is inoculated in 9K culture medium by 10% (v/v) inoculum concentration, and is cultivated to 9K
The circuit board that concentration is 10g/L is added in base, is 35 DEG C in cultivation temperature, shaking speed 180rpm, initial Fe2+Concentration is
20g/L, pH value leach 28 days under conditions of being 2.25, take out conical flask later, and rocking mixes precipitating, staticly settles stabilization
Afterwards, 0.1mL leachate is taken to be diluted to 60mL with distilled water, with kit (the triumphant microorganism Science and Technology Ltd. in Guangdong) to leaching liquor
In copper ion, nickel ion is measured.
Measurement result: the leaching rate of copper is 78.97%.The leaching rate of nickel is 61.13%.
Embodiment 6
Ferrous oxide complex microbial inoculum is by a kind of Thiobacillus ferrooxidans microorganism and a kind of ferrous oxide hook end spiral shell
Bacteria microorganism composition is revolved, bacterial strain uses therefor is the Thiobacillus ferrooxidans microorganism and deposit number that deposit number is ATCC23270 respectively
For the ferrous oxide hook end spiral bacteria microorganism of ATCC29047, contain Thiobacillus ferrooxidans microorganism and ferrous oxide hook end
The thalline quantity ratio of spiral bacteria microorganism is 3:1.
Ferrous oxide complex microbial inoculum is inoculated in 9K culture medium by 10% (v/v) inoculum concentration, and is cultivated to 9K
The circuit board that concentration is 10g/L is added in base, is 35 DEG C in cultivation temperature, shaking speed 180rpm, initial Fe2+Concentration is
20g/L, pH value leach 28 days under conditions of being 2.25, take out conical flask later, and rocking mixes precipitating, staticly settles stabilization
Afterwards, 0.1mL leachate is taken to be diluted to 60mL with distilled water, with kit (the triumphant microorganism Science and Technology Ltd. in Guangdong) to leaching liquor
In copper ion, nickel ion is measured.
Measurement result: the leaching rate of copper is 79.53%.The leaching rate of nickel is 62.82%.
Preferably to embody this complex microbial inoculum and application thereof, spy increases comparative example to highlight this microbial composite bacteria
Agent matches effect.
Comparative example 1
A kind of Thiobacillus ferrooxidans microorganism is contained only in microbial inoculum.
9K culture medium: 0.3g (NH4)2SO4, 0.1gKCl, 0.5gK2HPO4, 0.5gMgSO4·7H2O, 0.01gCa (NO3)2,
30gFeSO4·7H2O and 1000mL distilled water, finally uses 0.5mol/LH2SO4PH value is adjusted to 2.25.
By 10% (v/v) inoculum concentration, by Thiobacillus ferrooxidans microorganism, (bacterial strain uses therefor is that deposit number is ATCC23270
Thiobacillus ferrooxidans microorganism) it is inoculated in 9K culture medium, and circuit board is added into 9K culture medium, it is 35 in cultivation temperature
DEG C, shaking speed 180rpm, initial Fe2+Concentration is 20g/L, and pH value is cultivated 100 hours under conditions of being 2.25.
Be added circuit board concentration be respectively 1g/L, 5g/L, 10g/L, 15g/L, 20g/L, 25g/L, 30g/L, 35g/L and
45g/L。
As a result, it has been found that circuit board concentration is being added as 0-20g/ in the microbial inoculum for containing only a kind of Thiobacillus ferrooxidans microorganism
Still can be with normal growth in the 9K culture medium of L, but when circuit board concentration is more than 20g/L, this ferrous oxide microorganism is not
It can growth.The circuit board tolerance rate for illustrating this microorganism is 20g/L.
Comparative example 2
A kind of ferrous oxide hook end spiral bacteria microorganism is contained only in microbial inoculum.
9K culture medium: 0.3g (NH4)2SO4, 0.1gKCl, 0.5gK2HPO4, 0.5gMgSO4·7H2O, 0.01gCa (NO3)2,
30gFeSO4·7H2O and 1000mL distilled water, finally uses 0.5mol/LH2SO4PH value is adjusted to 2.25.
By 10% (v/v) inoculum concentration, by iron oxidation hook end spiral microorganism, (bacterial strain uses therefor is that deposit number is ATCC29047
Ferrous oxide hook end spiral bacteria microorganism) it is inoculated in 9K culture medium, and circuit board is added into 9K culture medium, in cultivation temperature
It is 35 DEG C, shaking speed 180rpm, initial Fe2+Concentration is 20g/L, and pH value is cultivated 100 hours under conditions of being 2.25.
Be added circuit board concentration be respectively 1g/L, 5g/L, 10g/L, 15g/L, 20g/L, 25g/L, 30g/L, 35g/L and
45g/L。
As a result, it has been found that this comparative example microbial inoculum still can be just in the 9K culture medium that circuit board concentration is 0-30g/L is added
It is frequently grown, but when circuit board concentration is more than 30g/L, this ferrous oxide microorganism cannot grow.Illustrate the electricity of this microorganism
Road plate tolerance rate is 30g/L.
Comparative example 3
Microbial inoculum contains only a kind of Thiobacillus ferrooxidans microorganism.
9K culture medium: 0.3g (NH4)2SO4, 0.1gKCl, 0.5gK2HPO4, 0.5gMgSO4·7H2O, 0.01gCa (NO3)2,
20gFeSO4·7H2O and 1000mL distilled water, finally uses 0.5mol/LH2SO4PH value is adjusted to 2.25.
By 10% (v/v) inoculum concentration, by microbial inoculum, (bacterial strain uses therefor is that the Thiobacillus ferrooxidans that deposit number is ATCC23270 is micro-
Biology) it is inoculated in 9K culture medium, and the circuit board that concentration is 10g/L is added into 9K culture medium, it is 35 DEG C in cultivation temperature,
Shaking speed is 180rpm, initial Fe2+Concentration is 20g/L, and pH value leaches 28 days under conditions of being 2.25, takes out taper later
Bottle, rocking mixes precipitating, staticly settles after stablizing, 0.1mL leachate is taken to be diluted to 60mL with distilled water, (wide with kit
Dong Kai microorganism Science and Technology Ltd.) copper ion, the nickel ion in leaching liquor are measured.
The leaching rate of measurement result copper is 71.23%.The leaching rate of nickel is 54.44%.
Comparative example 4
A kind of ferrous oxide hook end spiral bacteria microorganism is contained only in microbial inoculum.
9K culture medium: 0.3g (NH4)2SO4, 0.1gKCl, 0.5gK2HPO4, 0.5gMgSO4·7H2O, 0.01gCa (NO3)2,
20gFeSO4·7H2O and 1000mL distilled water, finally uses 0.5mol/LH2SO4PH value is adjusted to 2.25.
By 10% (v/v) inoculum concentration, by microbial inoculum, (bacterial strain uses therefor is the ferrous oxide hook end spiral that deposit number is ATCC29047
Bacteria microorganism) it is inoculated in 9K culture medium, and the circuit board that concentration is 10g/L is added into 9K culture medium, it is in cultivation temperature
35 DEG C, shaking speed 180rpm, initial Fe2+Concentration is 20g/L, and pH value is leached 28 days under conditions of being 2.25, taken out later
Conical flask, rocking mixes precipitating, staticly settles after stablizing, takes 0.1mL leachate to be diluted to 60mL with distilled water, use kit
(the triumphant microorganism Science and Technology Ltd. in Guangdong) is measured copper ion, the nickel ion in leaching liquor.
Measurement result: the leaching rate of copper is 68.17%.The leaching rate of nickel is 59.89%.
Comparative example 5
It is CCTCC NO:M's 2017687 that Thiobacillus ferrooxidans microorganism in embodiment 2, which is replaced with deposit number,
(for commercially available bacterial strain, the public can buy at China typical culture collection center (CCTCC) and obtain Thiobacillus ferrooxidans microorganism
).Remaining is same as Example 2.Measurement result: the leaching rate of copper is 66.54%.The leaching rate of nickel is 57.83%.
Comparative example 6
It is CCTCC NO:M that ferrous oxide hook end spiral bacteria microorganism in embodiment 2, which is replaced with deposit number,
2014195 ferrous oxide hook end spiral bacteria microorganism is commercially available bacterial strain, and the public can be in China typical culture collection center
(CCTCC) purchase obtains.Remaining is same as Example 2.Measurement result: the leaching rate of copper is 65.33%.The leaching rate of nickel is
54.27%.
1 it can be seen that this compound bacteria significantly improves the tolerance of circuit board with comparative example 1, comparative example 2 in conjunction with the embodiments；
2-6 and comparative example 3, comparative example 4 can be seen that this complex microbial inoculum to the metal leaching effect of circuit board in conjunction with the embodiments
It significantly improves.
The result of comparative example 5, comparative example 6 and embodiment 2 can be seen that the bacterial strain of the suitable deposit number of the application selection
Manufactured composite bacteria agent can be further improved the metal leaching effect of circuit board.
Complex microbial inoculum of the present invention has the advantages that environmental-friendly, tolerance is strong, and adaptability is high.Institute of the present invention
Stating complex microbial inoculum can be normal growth in the 9K culture medium of 35g/L in circuit board concentration.Therefore microorganism of the present invention
The metal that composite bacteria agent is suitable for circuit board under different condition leaches.To realize the utilization of board metal resource secondary, circulation benefit
It is contributed with, effective protection mining area ecological environment, while reaching and obtaining economic benefit and environmental-friendly win-win situation, and this
Invention complex microbial inoculum green non-pollution less will cause secondary pollution, therefore this hair after metal in leaching circuit board
Bright ideal efficient complex microbial inoculum in terms of being biological metallurgy, can be widely applied to biological metallurgy field.
In conclusion being only presently preferred embodiments of the present invention, limitation in any form not is done to the present invention, it is any ripe
Professional and technical personnel is known, without departing from the scope of the present invention, according to the technical essence of the invention, to the above reality
Any simple modifications, equivalent substitutions and improvements etc. that example is done are applied, within category technical solution of the present invention protection scope.
Claims (10)
1. a kind of ferrous oxide complex microbial inoculum, which is characterized in that sub- including Thiobacillus ferrooxidans microorganism and oxidation
Iron hook end spiral bacteria microorganism.
2. a kind of ferrous oxide complex microbial inoculum according to claim 1, which is characterized in that the ferrous oxide sulphur bar
Bacteria microorganism is selected from the following are one or more of in the microorganism of 44 kinds of deposit numbers；The microorganism of 44 kinds of deposit numbers is respectively as follows:
CCTCCAB206199、CCTCCAB206200、CCTCCAB206201、CCTCCAB206202、CCTCCAB206203、
CCTCCAB206204、CCTCCAB206205、CCTCCAB206206、CCTCCAB206207、CCTCCAB206208、
CCTCCAB207053、CCTCCAB207054、CCTCCAB207055、CCTCCAB207056、CCTCCAB207057、
CCTCCAB207058；ATCC13598D,ATCC53987,ATCC23270,ATCC53982,ATCC53983,ATCC14119,
ATCC19859,ATCC33020,ATCC21834,ATCC13728,ATCC13661；DSM-2390,DSM-1931,DSM-
14882、DSM-1928、DSM-1929、DSM-2389、DSM-700、DSM-2613、DSM-29444、DSM-9464、DSM-
11477、DSM-24419、DSM-9465、DSM-1927、DSM-584、DSM-583、DSM-585。
3. a kind of ferrous oxide complex microbial inoculum according to claim 1, which is characterized in that the ferrous oxide hook end
It is one or more of in microorganism of the spiral bacteria microorganism selected from following 20 kinds of deposit numbers；The microorganism of 20 kinds of deposit numbers is respectively as follows:
CCTCCAB206158、CCTCCAB206159、CCTCCAB206160、CCTCCAB206161、CCTCCAB206162、
CCTCCAB206163,CCTCCAB206164,CCTCCAB207036,CCTCCAB207037,CCTCCAB207038；
ATCC53992,ATCC29047,ATCC49879,ATCC49880,ATCC53993；DSM-2391,DSM-2705,DSM-1937,
DSM-1929、DSM-1928。
4. any one of -3 a kind of ferrous oxide complex microbial inoculum according to claim 1, which is characterized in that ferrous oxide
The thalline quantity ratio of Thiobacillus microorganism and ferrous oxide hook end spiral bacteria microorganism is (1-3): (3-1).
5. a kind of ferrous oxide complex microbial inoculum according to claim 4, which is characterized in that Thiobacillus ferrooxidans is micro-
The thalline quantity ratio of biology and ferrous oxide hook end spiral bacteria microorganism is 1:1 or 1:2 or 1:3 or 2:1 or 3:1.
6. any one of the claim 1-5 ferrous oxide complex microbial inoculum is leaching the application in metal.
7. a kind of method for leaching metal using any one of the claim 1-5 ferrous oxide complex microbial inoculum, special
Sign is, comprising the following steps: any one of the claim 1-5 ferrous oxide complex microbial inoculum is seeded in 9K culture
In base, circuit board is added in 9K culture medium, fermented and cultured.
8. leaching metal process according to claim 7, which is characterized in that when fermented and cultured, cultivation temperature 35-45
℃。
9. leaching metal process according to claim 7, which is characterized in that in 9K culture medium, initial Fe2+Concentration is 10-
30g/L。
10. according to the described in any item leaching metal process of claim 7-9, which is characterized in that initial pH value of medium is
2.0-3.0, time of fermented and cultured are 20-30 days, and shaking speed is 180rpm when fermented and cultured.
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| CN114231453B (en) * | 2021-12-17 | 2023-06-27 | 黑龙江八一农垦大学 | Thiobacillus ferrooxidans derived from volcanic ash and method for leaching metals |
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Application publication date: 20190416 |