CN105199990A - Autotrophic and heterotrophic compound ore-leaching flora FIM-Z4 and application thereof - Google Patents
Autotrophic and heterotrophic compound ore-leaching flora FIM-Z4 and application thereof Download PDFInfo
- Publication number
- CN105199990A CN105199990A CN201510674270.5A CN201510674270A CN105199990A CN 105199990 A CN105199990 A CN 105199990A CN 201510674270 A CN201510674270 A CN 201510674270A CN 105199990 A CN105199990 A CN 105199990A
- Authority
- CN
- China
- Prior art keywords
- leaching
- fim
- ore
- heterotrophic
- acidithiobacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/20—Recycling
Abstract
The invention discloses autotrophic and heterotrophic compound ore-leaching flora FIM-Z4 and application thereof and provides a mixed culture FIM-Z4 of acidithiobacillus ferrooxidans, acidithiobacillus thiooxidans and acidiphiliu acidophilum; the preservation number of the mixed culture FIM-Z4 is CGMCC No.11190. Through synergistic effect, the leaching reaction kinetics is improved obviously, the reaction speed is increased, the leaching period is shortened, and the leaching rate and leaching yield of metal (copper and nickel) ions in target ore are increased. The heterotrophic compound ore-leaching flora FIM-Z4 is a compatible whole, and the population ecology keeps relatively stable in a long-term domestication process. The heterotrophic compound ore-leaching flora FIM-Z4 is applied to biological metallurgy and has a better leaching effect on copper ions in low-grade copper sulphide ore and nickel ions in low-grade nickel ore.
Description
Technical field:
The invention belongs to field of metallurgy, be specifically related to a kind of autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 and application thereof.
Background technology:
Biohydrometallurgy is a kind of technique with microorganism, the direct, indirect of ore or both actings in conjunction being leached to useful metal in ores.Compared with conventional metallurgical technology, biological metallurgy has that cost is low, technical process is simple, environment amenable advantage, and can process traditional metallurgical method cannot or reluctant low grade ore, raw ore and mine tailing etc.In order to adapt to the efficiency of different ore type and raising bacterial leaching, finding, gathering and screen efficient, adaptable, that selectivity is good leaching ore deposit bacterium is the key of carrying out biological metallurgy fundamental research and through engineering approaches application.
If at present for soaking the microbial host in ore deposit addicted to acid, aerobic autotrophic type thiobacillus, wherein thiobacillus ferrooxidant (Acidithiobacillusferrooxidans, A.f) be most study and be a most important class leaching microbacteria in current Bioleaching correlative study, A.f obtains the energy and fixation of C O with iron protoxide and (or) reduced form sulfide
2as carbon source battalion chemoautotrophic growth.Acidithiobacillus thiooxidans (Acidithiobacillusthiooxidans, A.t) is obligate autotrophy microorganism, can oxidation of elemental sulfur, thiosulphate and chain four vitriol.In the bioleaching process of sulfide ore, A.t lays under tribute, and the A.f that generally accompanys plays a role jointly.
The general poor growth of autotrophic type thiobacillus, this is also the inefficient major cause in leaching ore deposit.Research shows, the growth of some meta-bolitess to himself of leaching microbacteria has restraining effect.
Summary of the invention:
The object of this invention is to provide one and can significantly improve Leaching reaction kinetics, fast reaction speed, shorten leaching cycle, improve autotrophic type and the heterotroph composite mineral-leaching bacteria colony FIM-Z4-Acidithiobacillus ferrooxidans strain GF (Acidithiobacillusferrooxidans) of the leaching yield of metal ion in target ore deposit, Acidithiobacillus thiooxidans (Acidithiobacillusthiooxidans), the mixed culture FIM-Z4 of acidophilic heterotrophic bacteria (Acidiphiliuacidophilum), it was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) on 09 08th, 2015, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is: CGMCCNo.11190.
Present invention also offers the application of mixed culture FIM-Z4 in leaching ore deposit of Acidithiobacillus ferrooxidans strain GF (Acidithiobacillusferrooxidans), Acidithiobacillus thiooxidans (Acidithiobacillusthiooxidans), acidophilic heterotrophic bacteria (Acidiphiliuacidophilum).The application in copper or nickel is preferably leached when soaking ore deposit.
The present invention obtains through enrichment culture and a kind ofly soaks by autotrophic type and heterotroph the autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 that ore deposit bacterium forms from the ore that copper-sulphide ores mine, Fujian gathers, the dominant bacteria mainly Acidithiobacillus ferrooxidans strain GF (Acidithiobacillusferrooxidans) of this autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4, Acidithiobacillus thiooxidans (Acidithiobacillusthiooxidans), pseudomonas aeruginosa (pseudomonassp.), acidophilic heterotrophic bacteria (Acidiphiliumacidophilum) and Sphingol single-cell (Sphingomonassp.).Compared with soaking ore deposit mixed bacterium with autotrophic type, this autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 can improve Leaching reaction kinetics, fast reaction speed significantly, shorten leaching cycle, improve target ore deposit (copper and mickel) Bioleaching speed and leaching yield.Autotrophic type of the present invention and heterotroph composite mineral-leaching bacteria colony FIM-Z4 are a compatible entirety, and in long-term domestication process, Population Ecological keeps relative stability.It is applied in biological metallurgy, has good leaching effect to the cupric ion in low-grade copper sulfide ores and the nickel ion in low grade nickel ore.
Containing the heterotrophic bacterium using organic substrates as energy substance in leaching ore body system, just can remove the restraining effect to autotrophic bacteria to a certain extent, be conducive to the growth of mainly soaking ore deposit bacterium, give full play to its leaching ore deposit effect, improve leaching yield.In mixing mineral-leaching bacteria colony heterotrophic bacterium greatly mainly with the meta-bolites of autotrophic bacteria, dead thalline as the growth energy, can nutritive substance be made full use of, remove meta-bolites to the suppression of autotrophic bacteria, increase the proliferative speed of autotrophic bacteria, and then improve Bioleaching rate.Autotrophic type and heterotroph leaching ore deposit bacterium are played an important role raising metal leaching rate and leaching yield by synergy.
The mixed culture FIM-Z4 of Acidithiobacillus ferrooxidans strain GF of the present invention (Acidithiobacillusferrooxidans), Acidithiobacillus thiooxidans (Acidithiobacillusthiooxidans), acidophilic heterotrophic bacteria (Acidiphiliuacidophilum) was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) on 09 08th, 2015, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is: CGMCCNo.11190.
Embodiment:
Following examples further illustrate of the present invention, instead of limitation of the present invention.
Embodiment 1: the enrichment of autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 and domestication
Get the low-grade copper sulfide ores sample ore that copper-sulphide ores mine, Fujian gathers, join in 9K-S substratum, 32 DEG C, 150rpm shakes cultivation, treats that nutrient solution becomes red-brown, and microscopy obtains 1st generation enrichment culture thing after observing and having a large amount of motion bacterium to exist.1st generation enrichment culture thing is turned culture, and method is the same, but bacterial load will reduce by generation, after turning culture 8 ~ 10 generation, obtains final enrichment culture thing.
Described 9K-S substratum compound method is as follows: A liquid: by (NH
4)
2sO
43.0g, K
2hPO
40.5g, KCl0.1g, MgSO
4.7H
2o0.5g, Ca (NO
3) 0.01g, join in 800mL deionized water, H
2sO
4regulate pH to 2.0,121 DEG C of autoclaving 20min, obtain A liquid; B liquid: by FeSO
4.7H
2o44.78g joins in 200mL deionized water, H
2sO
4regulate pH to 2.0, aperture is that the membrane filtration of 0.22 μm is degerming; C: elemental sulfur powder 5g, adopt interrupter method sterilizing.A liquid after 800ml sterilizing and 200mlB liquid are added in C, after mixing, namely obtains 9K-S substratum.
Carry out ore domestication to above-mentioned enrichment culture thing to cultivate, the substratum of first culture is 9K-S substratum, 32 DEG C, 150rpm shakes cultivation, domestication cultivation 6 generation, reduce the content of ferrous sulfate and sulphur powder in above-mentioned 9K-S substratum by generation, increase the content of low-grade copper sulfide ores sample ore as energy substance simultaneously, thus improve enriched substance to the adaptive faculty in target ore deposit.Each shake-flask culture time is 7 ~ 10d, and microscopy can be observed a large amount of motion bacterium and exist.No longer add ferrous sulfate and sulphur powder in last domestication process, ore becomes unique energy substance.In 9K substratum (without Fe), finally add low-grade copper sulfide ores sample ore, as unique energy source material, repeatedly culture is turned to flora, obtain the stable autotrophic type through ore domestication and heterotroph composite mineral-leaching bacteria colony thus, be autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4.
Embodiment 2: the compositional analysis of autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 bacterial population
Adopt the biological community structure of the autotrophic type after IlluminaMiSeq high throughput sequencing technologies analysis domestication and heterotroph composite mineral-leaching bacteria colony FIM-Z4.Key step is: adopt CTAB/NaCl method to extract the STb gene of autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4,1% agarose gel electrophoresis is utilized to detect the genomic dna integrity of extracting, the V3 district of amplification bacterial 16 S rRNA, high-flux sequence is carried out by IlluminaMiseq order-checking platform, extract high quality sequence, bioinformatic analysis is carried out to sequence, obtains the situation of structure of community and abundance.Result shows, the dominant bacteria mainly Acidithiobacillus ferrooxidans strain GF (Acidithiobacillusferrooxidans) (abundance accounting 80.03%) of this autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4, Acidithiobacillus thiooxidans (Acidithiobacillusthiooxidans) (abundance accounting 9.02%), pseudomonas aeruginosa (pseudomonassp.) (abundance accounting 7.07%), acidophilic heterotrophic bacteria (Acidiphiliuacidophilum) (abundance accounting 1.80%) and Sphingol single-cell (Sphingomonassp.) (abundance accounting 1.11%).
By this autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 called after: Acidithiobacillus ferrooxidans strain GF (Acidithiobacillusferrooxidans), Acidithiobacillus thiooxidans (Acidithiobacillusthiooxidans), the mixed culture FIM-Z4 of acidophilic heterotrophic bacteria (Acidiphiliuacidophilum), China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) is preserved on 09 08th, 2015, address: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, deposit number is: CGMCCNo.11190.
Embodiment 3: the leaching test of low-grade copper sulfide ores
With certain low-grade copper sulfide ores for sample ore, the granularity ﹤ 0.075mm of sample ore, essential mineral composition is chalcopyrite, purple copper, pyrite and lead glance, and copper grade is 0.47%.
By autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 (i.e. above-mentioned Acidithiobacillus ferrooxidans strain GF (Acidithiobacillusferrooxidans), Acidithiobacillus thiooxidans (Acidithiobacillusthiooxidans), the mixed culture FIM-Z4 of acidophilic heterotrophic bacteria (Acidiphiliuacidophilum)) and the contrast bacterium (mixed bacterium of Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans, both content in mixed bacterium is consistent with the content of the Acidithiobacillus ferrooxidans strain GF in autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 and Acidithiobacillus thiooxidans) access in 9K-S substratum (with embodiment 1) respectively, 32 DEG C, 150rpm microscopy is observed and cell counting finds, autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 are through of short duration lag phase, just logarithmic phase is entered rapidly.By bacterium liquid centrifugal 20min under 8000r/min, abandon supernatant liquor and be precipitated, with the sulphuric acid soln washing precipitation that pH2.0 is sterilized in advance, then centrifugal reject supernatant liquor, repeatable operation like this three times, to reach Fe in bacteria-removing liquid
3+object, obtain iron-free cell suspension, count under biomicroscope, adjustment cell concn be 1 × 10
9cellmL
-1.Get 9K substratum (not containing Fe
2+, namely in conventional 9K substratum, do not add FeSO
4.7H
2o, other components unchanged) 180mL is placed in 500mL Erlenmeyer flask, and take 20g sample ore and put into Erlenmeyer flask, sulfuric acid regulates slurry pH, controls initial pH value 1.8 ~ 2.0.Adding above-mentioned cell concn of adjusting is 1 × 10
9cellmL
-1autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 (volume fraction 5% inoculum size), 32 DEG C, 150rpm shakes cultivation.Simultaneously not add leaching ore deposit bacterium as blank, to add the mixed bacterium of above-mentioned Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans according to same treatment in contrast.Acid consumption in leaching process is supplemented by the sulfuric acid of 10mol/L, and the moisture of vaporization losses supplements by adding distilled water.The Cu in leach liquor is measured after leaching 20d
2+concentration.Blank group Cu
2+leaching yield is 17%, adopts the mixed bacterium of Acidithiobacillus ferrooxidans strain GF and oxidation sulfurous thiobacillus, Cu
2+leaching yield is 52%, and adopts autotrophic type of the present invention and heterotroph composite mineral-leaching bacteria colony FIM-Z4, Cu
2+leaching yield can reach 73%.Show the effect only relying on acidleach, Cu
2+leaching effect is more weak, and by soaking the effect of ore deposit bacterium, Cu
2+leaching effect obviously strengthens.With apply separately the autotrophic type leaching microbacteria mixed bacterium of Acidithiobacillus thiooxidans (Acidithiobacillus ferrooxidans strain GF with) and compare, autotrophic type and heterotroph leaching ore deposit composite bacteria (autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4) pass through synergy, Cu
2+leaching effect significantly strengthens.
Described 9K liquid nutrient medium compound method is as follows: A liquid: (NH
4)
2sO
43.0g, K
2hPO
40.5g, KCl0.1g, MgSO
4.7H
2o0.5g, Ca (NO
3), 0.01g, 800mL deionized water dissolving, H
2sO
4regulate pH to 2.0,121 DEG C of autoclaving 20min; B liquid: FeSO
4.7H
2o44.78g, deionized water 200mL dissolve, H
2sO
4regulate pH to 2.0, aperture is that the membrane filtration of 0.22 μm is degerming.After A liquid after sterilizing and B liquid being mixed, packing is stand-by.
Embodiment 4: the leaching test of low-grade nickel sulfide ore
With certain low-grade nickel sulfide ore for sample ore, the granularity ﹤ 0.075mm of sample ore, essential mineral composition is nickel pyrrhotite, pentlandite and chalcopyrite.Ore nickel grade is 0.73%.
By autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 (i.e. above-mentioned Acidithiobacillus ferrooxidans strain GF (Acidithiobacillusferrooxidans), Acidithiobacillus thiooxidans (Acidithiobacillusthiooxidans), the mixed culture FIM-Z4 of acidophilic heterotrophic bacteria (Acidiphiliuacidophilum)) and the contrast bacterium (mixed bacterium of Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans, both content in mixed bacterium and the Acidithiobacillus ferrooxidans strain GF in autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 and Acidithiobacillus thiooxidans consistent) access in 9K-S substratum (with embodiment 1) respectively, 32 DEG C, 150rpm cultivates, microscopy is observed and cell counting finds, autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 are through of short duration lag phase, just logarithmic phase is entered rapidly.By bacterium liquid centrifugal 20min under 8000r/min, abandon supernatant liquor and be precipitated, with the sulphuric acid soln washing precipitation that pH2.0 is sterilized in advance, then centrifugal reject supernatant liquor, repeatable operation like this three times, to reach Fe in bacteria-removing liquid
3+object, obtain iron-free cell suspension, count under biomicroscope, adjustment cell concn be 1 × 10
9cellmL
-1.Get 9K substratum (not containing Fe
2+) 180mL is placed in 500mL Erlenmeyer flask, takes 20g sample ore and puts into Erlenmeyer flask, sulfuric acid regulates slurry pH, controls initial pH value 1.8 ~ 2.0.Adding above-mentioned cell concn of adjusting is 1 × 10
9cellmL
-1autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 (volume fraction 5% inoculum size), 32 DEG C, 150rpm shakes cultivation.Simultaneously not add leaching ore deposit bacterium as blank, to add the mixed bacterium of above-mentioned Acidithiobacillus ferrooxidans strain GF and Acidithiobacillus thiooxidans according to same process in contrast.Acid consumption in leaching process is supplemented by the sulfuric acid adding 10mol/L, and the moisture of vaporization losses is by adding 9K culture medium supplemented.After leaching 20d, blank group Ni
2+leaching yield is 12%, adopts the mixed bacterium of Acidithiobacillus ferrooxidans strain GF and oxidation sulfurous thiobacillus, Ni
2+leaching yield is 51%, and adopts autotrophic type of the present invention and heterotroph composite mineral-leaching bacteria colony FIM-Z4, Ni
2+leaching yield can reach 70%.Above result shows the effect only relying on acidleach, Ni
2+leaching effect is faint, and by soaking the effect of ore deposit bacterium, Ni
2+leaching effect obviously strengthens.With apply separately the autotrophic type leaching microbacteria mixed bacterium of Acidithiobacillus thiooxidans (Acidithiobacillus ferrooxidans strain GF with) and compare, autotrophic type and heterotroph leaching ore deposit composite bacteria (autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4) pass through synergy, Ni
2+immersion Zn successful strengthens.
Claims (3)
1. the mixed culture FIM-Z4 of Acidithiobacillus ferrooxidans strain GF (Acidithiobacillusferrooxidans), Acidithiobacillus thiooxidans (Acidithiobacillusthiooxidans), acidophilic heterotrophic bacteria (Acidiphiliuacidophilum), its deposit number is: CGMCCNo.11190.
2. the application of mixed culture FIM-Z4 in leaching ore deposit of Acidithiobacillus ferrooxidans strain GF according to claim 1 (Acidithiobacillusferrooxidans), Acidithiobacillus thiooxidans (Acidithiobacillusthiooxidans), acidophilic heterotrophic bacteria (Acidiphiliuacidophilum).
3. application according to claim 2, it is characterized in that, the mixed culture FIM-Z4 of Acidithiobacillus ferrooxidans strain GF (Acidithiobacillusferrooxidans), Acidithiobacillus thiooxidans (Acidithiobacillusthiooxidans), acidophilic heterotrophic bacteria (Acidiphiliuacidophilum) leaches the application in copper or nickel when soaking ore deposit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510674270.5A CN105199990B (en) | 2015-10-16 | 2015-10-16 | A kind of autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510674270.5A CN105199990B (en) | 2015-10-16 | 2015-10-16 | A kind of autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105199990A true CN105199990A (en) | 2015-12-30 |
| CN105199990B CN105199990B (en) | 2018-06-01 |
Family
ID=54947953
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510674270.5A Active CN105199990B (en) | 2015-10-16 | 2015-10-16 | A kind of autotrophic type and heterotroph composite mineral-leaching bacteria colony FIM-Z4 and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105199990B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105969695A (en) * | 2016-06-22 | 2016-09-28 | 福建省微生物研究所 | Mineral leaching compound bacterium FIM-S1 and application thereof to high-grade copper sulfide mineral leaching |
| CN111808773A (en) * | 2020-07-22 | 2020-10-23 | 东华理工大学 | Acidithiobacillus ferrooxidans and application thereof and ore leaching method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101481667A (en) * | 2008-10-28 | 2009-07-15 | 李红玉 | A strain of acidophilic heterotrophic bacteria DX-X and use thereof |
| CN103572050A (en) * | 2013-11-19 | 2014-02-12 | 东北大学 | Biological selectivity leaching method of low-grade copper-cobalt ore |
| CN103667131A (en) * | 2013-12-05 | 2014-03-26 | 中国科学院微生物研究所 | Method for increasing metal ore leaching rate, and special strain thereof |
| CN104152891A (en) * | 2014-08-20 | 2014-11-19 | 太原理工大学 | Method for rapidly forming metallurgical alloy layer on magnesium alloy surface |
| WO2015075507A1 (en) * | 2013-11-22 | 2015-05-28 | Kone Corporation | Bearing block cover, axial flux motor, elevator and method of compensating manufacturing tolerances in an axial flux motor |
-
2015
- 2015-10-16 CN CN201510674270.5A patent/CN105199990B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101481667A (en) * | 2008-10-28 | 2009-07-15 | 李红玉 | A strain of acidophilic heterotrophic bacteria DX-X and use thereof |
| CN103572050A (en) * | 2013-11-19 | 2014-02-12 | 东北大学 | Biological selectivity leaching method of low-grade copper-cobalt ore |
| WO2015075507A1 (en) * | 2013-11-22 | 2015-05-28 | Kone Corporation | Bearing block cover, axial flux motor, elevator and method of compensating manufacturing tolerances in an axial flux motor |
| CN103667131A (en) * | 2013-12-05 | 2014-03-26 | 中国科学院微生物研究所 | Method for increasing metal ore leaching rate, and special strain thereof |
| CN104152891A (en) * | 2014-08-20 | 2014-11-19 | 太原理工大学 | Method for rapidly forming metallurgical alloy layer on magnesium alloy surface |
Non-Patent Citations (5)
| Title |
|---|
| PAUL R. NORRIS 等: "Autotrophic, sulfur-oxidizing actinobacteria in acidic environments", 《EXTREMOPHILES》 * |
| 张晓雪: "嗜酸异养细菌的分离、鉴定以及应用", 《中国优秀硕士学位论文全文数据库 基础科学辑》 * |
| 邬长斌: "中等嗜热菌浸出黄铜矿的研究", 《中国博士学位论文全文数据库》 * |
| 邹俐宏等: "源自硫化矿区的Acidiphilium属菌的分离及其浸矿性能", 《中国有色金属学报》 * |
| 陈明莲: "微生物对黄铜矿表面性质的影响及其吸附机制的研究", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105969695A (en) * | 2016-06-22 | 2016-09-28 | 福建省微生物研究所 | Mineral leaching compound bacterium FIM-S1 and application thereof to high-grade copper sulfide mineral leaching |
| CN105969695B (en) * | 2016-06-22 | 2019-05-07 | 福建省微生物研究所 | A kind of leaching mine compound bacteria FIM-S1 and its application in high-grade copper-sulphide ores leaching mine |
| CN111808773A (en) * | 2020-07-22 | 2020-10-23 | 东华理工大学 | Acidithiobacillus ferrooxidans and application thereof and ore leaching method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105199990B (en) | 2018-06-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wang et al. | Enhanced bioleaching efficiency of metals from E-wastes driven by biochar | |
| CN101195859B (en) | Technique for processing low concentration heavy metal sulfate liquor with microorganism method | |
| CN102174425B (en) | Acidithiobacillus and application thereof | |
| CN103993171B (en) | A kind of method of adding nonionogenic tenside promotion chalcopyrite Bioleaching | |
| CN105734285B (en) | A kind of method for strengthening zincblende Microorganism Leaching | |
| CN104862250B (en) | Acidophilic iron-oxidizing composite microbial agent and preparation method thereof | |
| CN102329957A (en) | Method for continuously leaching sulfide ore by using synergy of autotrophic ore leaching bacteria and heterotrophic ore leaching bacteria | |
| CN104877933A (en) | Bacterial strains capable of being used for preparing acidophilic iron oxidizing microbial compound agent and application thereof | |
| CN105861823A (en) | Method for reinforcing leaching of chalcopyrite microorganisms | |
| CN103013847B (en) | Ammonia-producing mineral leaching bacterium as well as culture method and application of ammonia-producing mineral leaching bacterium | |
| CN103131650B (en) | Eosino-thiobacillus and application thereof to copper pyrites leaching | |
| CN107794368A (en) | A kind of method based on microorganism growth and chemical regulation enhancing Leaching of chalcopyrite | |
| CN108102970B (en) | Mixotrophic microorganism functional flora for removing acid-soluble cadmium and reducible cadmium in cadmium-polluted soil and preparation and application methods thereof | |
| CN104862474A (en) | Method for biologically leaching heavy metal from heavy metal-contained waste based on pH and potential co-regulation | |
| CN103667131B (en) | A kind of method and special strain therefore thereof improving metallic ore leaching rate | |
| CN105199990A (en) | Autotrophic and heterotrophic compound ore-leaching flora FIM-Z4 and application thereof | |
| CN101016583A (en) | Method of eliminating passivation film for chalcopyrite at lixiviating course by sulfur oxidizing bacteria | |
| CN102162029B (en) | Microbiological oxidation and reduction coupling leaching method for valuable metal in manganese oxide ore | |
| CN106635854A (en) | Oligographic bacterium composition and application of oligographic bacterium composition in inhibition on generation of jarosite in biological leaching | |
| CN101805829A (en) | Selective bioleaching process of high-sulphur/copper ratio secondary copper sulphide ore | |
| CN105969695B (en) | A kind of leaching mine compound bacteria FIM-S1 and its application in high-grade copper-sulphide ores leaching mine | |
| CN105255763B (en) | A method of microorganism adhesion effect in enhancing brass mineral biological lixiviation process | |
| CN103540519B (en) | Double-layer flat plate and preparation method thereof | |
| CN103114209A (en) | Method for leaching cobalt and copper in cobalt-copper alloy through cooperation of thiobacillus ferrooxidans | |
| CN102965303A (en) | Breeding method for cobalt leaching bacteria |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |