CN102174425B - Acidithiobacillus and application thereof - Google Patents

Acidithiobacillus and application thereof Download PDF

Info

Publication number
CN102174425B
CN102174425B CN201010602520A CN201010602520A CN102174425B CN 102174425 B CN102174425 B CN 102174425B CN 201010602520 A CN201010602520 A CN 201010602520A CN 201010602520 A CN201010602520 A CN 201010602520A CN 102174425 B CN102174425 B CN 102174425B
Authority
CN
China
Prior art keywords
liking
acid oxidase
ferrous thiobacillus
thiobacillus
molybdenum
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201010602520A
Other languages
Chinese (zh)
Other versions
CN102174425A (en
Inventor
孟运生
郑英
樊保团
周磊
程浩
刘辉
师留印
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beijing Research Institute of Chemical Engineering and Metallurgy of CNNC
Original Assignee
Beijing Research Institute of Chemical Engineering and Metallurgy of CNNC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Beijing Research Institute of Chemical Engineering and Metallurgy of CNNC filed Critical Beijing Research Institute of Chemical Engineering and Metallurgy of CNNC
Priority to CN201010602520A priority Critical patent/CN102174425B/en
Publication of CN102174425A publication Critical patent/CN102174425A/en
Application granted granted Critical
Publication of CN102174425B publication Critical patent/CN102174425B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P10/00—Technologies related to metal processing
    • Y02P10/20—Recycling

Abstract

The invention relates to an acidithiobacillus and application thereof. The preserving number of acidithiobacillus ferrooxidans SWLM1 of the acidithiobacillus is CGMCC No.3265. The invention also provides the application of the acidithiobacillus ferrooxidans in metallurgy, particularly the acidithiobacillus ferrooxidans is applied to the leaching of molybdenum ore containing Fe2<+> or sulfides. In the invention, the acidithiobacillus ferrooxidans is added into the molybdenum ore pulp containing the Fe2<+>, the leaching temperature is between 28 DEG C and 32 DEG C, the leaching pH is between 1 and 3, and the Fe2<+> is oxidized into Fe3<+>; the acidithiobacillus ferrooxidans is also added into the molybdenum ore pulp containing Fe2<+> and sulfides, the leaching temperature is between 28 DEG C and 32 DEG C, the leaching pH is between 1 and 3, and the Fe2<+> and the sulfides are oxidized into Fe3<+> and sulfuric acid or sulfate respectively. The acidithiobacillus ferrooxidans provided by the invention has a stronger molybdenum resisting characteristic, can also grow well when the content of the molybdenum is 1.6 g/L, and can be widely applied to the leaching of molybdenum-bearing minerals.

Description

One strain thiobacillus ferrooxidans and application thereof
Technical field
The acidifying of having a liking for that the present invention relates to be used for extraction and the recovery of metallic ore metal can autotrophic bacteria, is specifically related to a strain and has a liking for ferrous thiobacillus of acid oxidase and application method thereof.
Background technology
The extraction and the recovery of metal in the low-grade metal ore during the bacterium leaching-out technique is usually used in.Ordinary person was used for the leaching of metal during conventional chemistry leached for a large amount of sulfuric acid of adding and oxygenant.Because the large usage quantity of sulfuric acid and oxygenant also improves the technology cost greatly.Have a liking for acidifying and can autotrophic bacteria overcome above deficiency and the bacterium leaching-out technique is capable of using.Have a liking for acidifying and can mainly contain ThiobaciLLus ferrooxidans (thiobacillus ferrooxidant) by autotrophic bacteria; ThiobaciLLus thiooxidans (thiobacillus thiooxidans); FerrobaciLLusferrooxidans (red stone Ferrobacillus); LeptospiriLLum ferrooxidans (iron protoxide hook end spiral shell fine jade bacterium) etc., they all can receive the influence of harmful ion concentration in the solution in soaking the ore deposit process.Harmful ion content too much will produce toxic action to bacterium in the solution.They belong to ion to GOLD FROM PLATING SOLUTION for the bacterium of in the uranium ore leaching process, using all has limit tolerance concentration.Ore-leaching bacteria is 0.16g/L to the limit tolerance concentration of molybdenum -1, this makes the application of bacterium leaching-out technique in uranium molybdenum ore and the leaching of pure molybdenum ore receive restriction.Screening and separating goes out the ore immersion bacterial culture that under high molybdenum environment, has greater activity and oxidation efficiency and has great application prospect.
Summary of the invention
The purpose of this invention is to provide that a strain can be grown in high molybdenum environment, have greater activity and oxidation efficiency have a liking for ferrous thiobacillus of acid oxidase and application method thereof.
Of the present inventionly have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidansSWLM1); Be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on September 7th, 2009; Address: Datun Road, Chaoyang District, Beijing City; Institute of Microorganism, Academia Sinica, preserving number CGMCC No.3265, preservation title Acidithiobacillus ferrooxidans SWLM1.
Of the present inventionly having a liking for the application of the ferrous thiobacillus of acid oxidase in metallurgy, is to be applied to contain Fe with having a liking for the ferrous thiobacillus of acid oxidase 2+Or the molybdenum ore of sulfide leaches.
Of the present inventionly having a liking for the method that the ferrous thiobacillus of acid oxidase is leached mineral, is this to be had a liking for the ferrous thiobacillus of acid oxidase join and contain Fe 2+Molybdenum ore slurry in, extraction temperature leaches pH 1～3, through having a liking for the ferrous thiobacillus of acid oxidase with Fe at 28～32 ℃ 2+Be oxidized to Fe 3+
Above-mentionedly this is had a liking for the ferrous thiobacillus of acid oxidase join and contain Fe 2+Molybdenum ore slurry in, extraction temperature leaches pH 2～2.5 at 30 ℃, utilizes CO 2Be carbon source, extraction time was at 20～30 days
Above-mentionedly have a liking for the method that the ferrous thiobacillus of acid oxidase is leached mineral, it will be had a liking for the ferrous thiobacillus of acid oxidase earlier and be inoculated in the 9K substratum, and the volume of having a liking for the ferrous thiobacillus of acid oxidase is 10～12% of a 9K culture volume, they is joined to contain Fe then 2+Molybdenum ore slurry in; Described 9K substratum comprises (NH 4) 2SO 40.3wt%, K 2HPO 40.05wt%, KCl 0.01wt%, MgSO 47H 2O 0.1wt%, FeSO 47H 2O 4.44wt%, it is at 120～121 ℃ of sterilization 20～25min.
Of the present inventionly having a liking for the method that the ferrous thiobacillus of acid oxidase is leached mineral, is this to be had a liking for the ferrous thiobacillus of acid oxidase join and contain Fe 2+In the molybdenum ore of sulfide slurry, extraction temperature leaches pH 1～3 at 28～32 ℃, has a liking for the ferrous thiobacillus of acid oxidase with Fe 2+, the sulfide selective oxidation is Fe 3+, sulfuric acid or vitriol.
Above-mentionedly this is had a liking for the ferrous thiobacillus of acid oxidase join and contain Fe 2+In the molybdenum ore of sulfide slurry, extraction temperature leaches pH 2～2.5 at 30 ℃, utilizes CO 2Be carbon source, extraction time was at 20～30 days.
Above-mentionedly have a liking for the method that the ferrous thiobacillus of acid oxidase is leached mineral, it will be had a liking for the ferrous thiobacillus of acid oxidase earlier and be inoculated in the 9K substratum, and the volume of having a liking for the ferrous thiobacillus of acid oxidase is 10～12% of a 9K culture volume, they is joined to contain Fe then 2+In the molybdenum ore of sulfide slurry; Described 9K substratum comprises (NH 4) 2SO 40.3wt%, K 2HPO 40.05wt%, KCl 0.01wt%, MgSO 47H 2O0.1wt%, FeSO 47H 2O 4.44wt%, it is at 120～121 ℃ of sterilization 20～25min.
Of the present inventionly have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidansSWLM1); Gramstaining is negative, and the opticmicroscope hypothallus is little, and is shaft-like; Can move; Transmission electron microscope is observed thalline down has single flagellum, and near-end is given birth to, and size is 0.4-0.6 μ m * 2-2.5 μ m.This has a liking for the ferrous thiobacillus of acid oxidase is typical acidophilia chemoautotrophic bacteria, through Oxidation of Fe 2+Obtain the energy growth with sulfide, product is Fe 3+, sulfuric acid, vitriol; Utilize CO 2Be carbon source; Optimum growth temperature is 30 ℃; The righttest growth pH is 2～2.5.
Of the present inventionly have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans SWLM1) and have stronger anti-molybdenum characteristic, when molybdenum element content 1.6g/L, still can better grow, can in the leaching of molybdenum-bearing mineral, be used widely.Of the present inventionly have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillusferrooxidans SWLM1) and can in environment protection, be used widely, as being applied to WWT etc.
Embodiment
Below in conjunction with accompanying drawing and specific embodiment to strain thiobacillus ferrooxidans of the present invention and should be as further describing.
Of the present invention to have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans SWLM1) be typical acidophilia chemoautotrophic bacteria, through Oxidation of Fe 2+Obtain the energy growth with sulfide, product is Fe 3+, sulfuric acid, vitriol; Utilize CO 2Be carbon source; Optimum growth temperature is 28～32 ℃; Being fit to growth pH is 1～3.This SWLM1 bacterial strain has stronger anti-molybdenum property, when molybdenum element content 1.6g/L, still can better grow.16sRNA gene order to the SWLM1 bacterial strain is carried out the systematics analysis, and its sequence similarity comparison and evolutionary relationship show that this bacterium is thiobacillus ferrooxidant (Acidithiobacillus ferrooxidans SWLM 1).
Of the present inventionly have a liking for the ferrous thiobacillus of acid oxidase (Acidithiobacillus ferrooxidans SWLM1) and separate in the pit water of 5 ore deposit point pH scope 5-6 on molybdenum ore mountain, Luanchuan, Luoyang and moistening earth.With enrichment medium the water sample and the soil sample of fetching are carried out enrichment culture, 30 ℃ of 90r/min shaking tables are cultured to the substratum color and become reddish-brown.Through 3 liquid switching enrichments, separation bacterial classification concentration and activity are greatly improved.
Used substratum is among the present invention:
Liquid 9K substratum: (NH 4) 2SO 43.0g, K 2HPO 40.5g, KCl 0.1g, MgSO 47H 2O1.0g, FeSO 47H 2O 44.4g, zero(ppm) water 1000ml, pH=2.0,121 ℃ of sterilization 20min.
Enrichment medium: the liquid 9K substratum of 2 times of concentration.
Used cultural method is: using the 9K culture medium inoculated amount of sterilization among the embodiment 1,2,3,4 is 10%, uses in the instance 5 that not contain ferrous 9K culture medium inoculated amount be 1%; Embodiment 2 shaking table under 28 ℃ of conditions is cultivated, and instance 4,5,6 shaking table under 30 ℃ of conditions is cultivated, and rotating speed is 90r/min.
Used analysis test method is: growth indexes OD value adopts spectrophotometer (WFZUV-2000 type spectrophotometer, Shanghai You Nike Instr Ltd.) to measure in the SWLM1 strain growth curve.
Embodiment 1
Use sterilization 9K substratum, with Fe 2+Be the energy, temperature is 28 ℃, and SWLM1 inoculation amount is 10%, carries out shaking table and cultivates.Survey OD in 0h, 5h, 10h, 12h, 15h, 18h, 20h, 22h, 25h, 28h, 30h, 32h, 35h and 40h sampling respectively 420, with not inoculating the 9K substratum as contrast.
Test-results shows: the SWLM1 bacterial strain remains on lag phase in 20h, and the thalline number slightly increases, but changes little.Begin to get into logarithmic phase behind the 20h, thalline quantity increases sharply.Behind 30h, reach stationary phase, thalline quantity remains on the peak state.
Embodiment 2
Use sterilization 9K substratum, with Fe 2+Be the energy, the inoculation amount is 10%, in temperature is 25～40 ℃ of scopes, carries out shaking table respectively simultaneously and cultivates, with not inoculating the 9K substratum as contrast.
Test-results shows: 28～32 ℃ are SWLM1 bacterial strain proper growth temperature, and 30 ℃ is the optimum growth temperature of SWLM1 bacterial strain.The culture temperature that is lower than 28 ℃ suppresses growth significantly to the SWLM1 bacterial strain; Be higher than 32 ℃ culture temperature, thalli growth also is suppressed.
Embodiment 3
Use sterilization 9K substratum, with Fe 2+Be the energy, temperature is 30 ℃, and the inoculation amount is 10%, in pH0.0～3.0 scopes, carries out shaking table respectively simultaneously and cultivates, with not inoculating the 9K substratum as contrast.
Test-results shows: the SWLM1 bacterial strain all can be grown in pH1.0～3.0, and the righttest growth pH is 2～2.5.Be lower than at pH under 1 the condition, thalli growth is almost stagnated; Be higher than at pH under 2.5 the condition, thalli growth also is suppressed.
Embodiment 4
In the nonferrous 100mL 9K substratum of sterilization, add 1. elemental sulfur 1g respectively; 2. FeSO 47H 2O 4.31g; 3. FeSO 47H 2O 4.31g+ elemental sulfur 1g; 4. Na 2S 2SO 35H 2O 1g (Na wherein 2S 2SO 35H 2O, FeSO 47H 2O adds substratum after all adopting 0.22 μ m ultrafiltration membrance filter degerming again, adds substratum behind the elemental sulfur water proof boiling 1h) as the energy:, temperature is 30 ℃, and inoculum size 1% is carried out shaking table simultaneously and is cultivated, and the substratum that does not connect bacterium with each group is as contrast.
Test-results shows: the SWLM1 bacterial strain is with Na 2S 2SO 3Can not grow during as unique energy; Can faint growth during as unique energy with S; With Fe 2+Growth is vigorous during as the energy.
Embodiment 5
Use the 9K substratum of sterilization, with Fe 2+Be the energy; Temperature is 30 ℃, and the inoculation amount is 10%, in substratum, adds the ammonium molybdate solution of filtration sterilization; Make molybdenum concentration be respectively 100mg/L, 200mg/L, 300mg/L, 400mg/L ....; Carry out shaking table and cultivate, observe the thalli growth situation, measure the redox potential of nutrient solution.Progressively improve the content of molybdenum in the substratum, the anti-molybdenum performance of naturalized strain.
Test-results shows: bacterial strain SWLM1 still can better grow in molybdenum concentration is the 9K substratum of 1.0g/L.Explain that this bacterial strain can adapt to higher molybdenum content, very strong anti-molybdenum ability is arranged.
Embodiment 6
135ml 9K substratum is added in the Erlenmeyer flask of 500ml; Add ammonium molybdate in this 9K substratum; Make the molybdenum concentration 800mg/L in the substratum, get the SWLM1 bacterium liquid that 15ml is in the growth logarithmic phase and join in the 9K substratum, cultivate at 30 ℃ of shaking tables; Measure the solution oxide reduction potential every day, observe the variation of solution colour.Test-results shows: the oxidation quickly of SWLM1 bacterial strain contains certain molybdenum concentration 9K substratum.
Table 1 contains the comparison test of certain molybdenum concentration 9K substratum oxidation rate
Embodiment 7
Get and contain Fe 2+Molybdenum ore slurry 30ml, Fe 2+Concentration is 5g/L; Add the 9K substratum of 105ml sterilization again, regulator solution pH is 2.5, adds the SWLM1 bacterium liquid that 15ml is in logarithmic phase then, and vibration is leached in the constant temperature shaking table of 30 degree, and shaking table was cultivated 30 days, measured the solution oxide reduction potential every day, do two groups parallel.
Table 2 is bacterium shake flask test results of above-mentioned molybdenum ore, can know from the result, and the molybdenum ore leaching effect is preferably SWLM1.
Single molybdenum ore 1 bacterium of table 2 shakes a bottle leaching test result
Figure GDA0000047135960000061
Embodiment 8
Get the 9K substratum that SWLM1 bacterium liquid that 10ml is in logarithmic phase joins the 100ml sterilization; Shaking culture adds the sulfuration molybdenum ore after one day again in the constant temperature shaking table of 30 degree, and pulp density is 5%～10%; Regulator solution pH is 2; Vibration was leached 20 days in the constant temperature shaking table of 30 degree, measured the solution oxide reduction potential every day, do two groups parallel.
Table 3 is bacterium shake flask test results of above-mentioned molybdenum ore, can know from the result, and the molybdenum ore leaching effect is preferably SWLM1.
Single molybdenum ore 2 bacteriums of table 3 shake a bottle leaching test result

Claims (8)

1. the ferrous thiobacillus of acid oxidase is had a liking in a strain, it is characterized in that: said culture presevation CGMCCNo.3265.
2. a claim 1 is described has a liking for the application of the ferrous thiobacillus of acid oxidase in metallurgy, and it is characterized in that: this is had a liking for the ferrous thiobacillus of acid oxidase and is applied to contain Fe 2+Molybdenum ore leach.
3. one kind is adopted that claim 1 is described has a liking for the method that the ferrous thiobacillus of acid oxidase is leached mineral, it is characterized in that: this is had a liking for the ferrous thiobacillus of acid oxidase join and contain Fe 2+Molybdenum ore slurry in, extraction temperature leaches pH 1～3, through having a liking for the ferrous thiobacillus of acid oxidase with Fe at 28～32 ℃ 2+Be oxidized to Fe 3+
4. according to claim 3ly have a liking for the method that the ferrous thiobacillus of acid oxidase is leached mineral, it is characterized in that: describedly this is had a liking for the ferrous thiobacillus of acid oxidase join and contain Fe 2+Molybdenum ore slurry in, extraction temperature leaches pH 2～2.5 at 30 ℃, utilizes CO 2Be carbon source, extraction time was at 20～30 days.
5. according to claim 3 or the 4 described methods of having a liking for the ferrous thiobacillus leaching of acid oxidase mineral; It is characterized in that: will have a liking for the ferrous thiobacillus of acid oxidase earlier and be inoculated in the 9K substratum; The volume of having a liking for the ferrous thiobacillus of acid oxidase is 10～12% of a 9K culture volume, they is joined to contain Fe then 2+Molybdenum ore slurry in; Described 9K substratum comprises (NH 4) 2SO 40.3wt%, K 2HPO 40.05wt%, KCl 0.01wt%, MgSO 47H 2O 0.1wt%, FeSO 47H 2O 4.44wt%, it is at 120～121 ℃ of sterilization 20～25min.
6. one kind is adopted that claim 1 is described has a liking for the method that the ferrous thiobacillus of acid oxidase is leached mineral, it is characterized in that: this is had a liking for the ferrous thiobacillus of acid oxidase join and contain Fe 2+In the molybdenum ore of sulfide slurry, extraction temperature leaches pH 1～3 at 28～32 ℃, has a liking for the ferrous thiobacillus of acid oxidase with Fe 2+, the sulfide selective oxidation is Fe 3+, sulfuric acid or vitriol.
7. according to claim 6ly have a liking for the method that the ferrous thiobacillus of acid oxidase is leached mineral, it is characterized in that: describedly this is had a liking for the ferrous thiobacillus of acid oxidase join and contain Fe 2+In the molybdenum ore of sulfide slurry, extraction temperature leaches pH 2～2.5 at 30 ℃, utilizes CO 2Be carbon source, extraction time was at 20～30 days.
8. according to claim 6 or the 7 described methods of having a liking for the ferrous thiobacillus leaching of acid oxidase mineral; It is characterized in that: will have a liking for the ferrous thiobacillus of acid oxidase earlier and be inoculated in the 9K substratum; The volume of having a liking for the ferrous thiobacillus of acid oxidase is 10～12% of a 9K culture volume, they is joined to contain Fe then 2+In the molybdenum ore of sulfide slurry; Described 9K substratum comprises (NH 4) 2SO 40.3wt%, K 2HPO 40.05wt%, KCl 0.01wt%, MgSO 47H 2O 0.1wt%, FeSO 47H 2O 4.44wt%, it is at 120～121 ℃ of sterilization 20～25min.
CN201010602520A 2010-12-23 2010-12-23 Acidithiobacillus and application thereof Active CN102174425B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201010602520A CN102174425B (en) 2010-12-23 2010-12-23 Acidithiobacillus and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201010602520A CN102174425B (en) 2010-12-23 2010-12-23 Acidithiobacillus and application thereof

Publications (2)

Publication Number Publication Date
CN102174425A CN102174425A (en) 2011-09-07
CN102174425B true CN102174425B (en) 2012-10-10

Family

ID=44517683

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201010602520A Active CN102174425B (en) 2010-12-23 2010-12-23 Acidithiobacillus and application thereof

Country Status (1)

Country Link
CN (1) CN102174425B (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102796689B (en) * 2012-09-04 2015-09-09 中国科学院成都生物研究所 A kind of cell culture processes of quick startup iron-oxidizing bacteria activity
CN103866122B (en) * 2014-01-06 2015-11-04 东华理工大学 A kind of uranium molten leaching of molybdenum ore microorganism and uranium molybdenum enrichment and separation method
CN105112346A (en) * 2015-10-15 2015-12-02 北京化工大学 Preparation method of improved 9k solid medium
CN105420488A (en) * 2015-12-30 2016-03-23 核工业北京化工冶金研究院 Method for preprocessing wrapped uranium-molybdenum ores through oxidizing roasting
CN106811198B (en) * 2016-12-30 2020-04-17 华南农业大学 Potassium farmland passivator based on molybdenum ore tailings as raw material and preparation method and application thereof
CN106811199B (en) * 2016-12-30 2020-04-17 华南农业大学 Farmland passivator based on molybdenum ore tailings as raw material and preparation method and application thereof
CN107699511B (en) * 2017-06-14 2020-04-24 中南大学 Thermophilic thiobacillus and culture medium and culture method thereof
CN107780278B (en) * 2017-11-17 2020-06-09 苏州博进生物技术有限公司 Microbial treatment method for waste chips generated by setting off fireworks and crackers during spring festival
CN115216627B (en) * 2021-04-21 2024-03-19 核工业北京化工冶金研究院 Application method of acid-resistant thiobacillus
CN115216418A (en) * 2021-04-21 2022-10-21 核工业北京化工冶金研究院 Application method of low-temperature-resistant thiobacillus

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20030092751A (en) * 2002-05-31 2003-12-06 한국과학기술원 Novel bacteria having the iron-oxidation activity and method for the leaching of heavy metals using the same bacteria
PE20060789A1 (en) * 2004-10-22 2006-08-10 Biosigma Sa WENELEN BACTERIA STRAIN DSM 16786 AND LEACHING PROCESS BASED ON INOCULATION OF SAID STRAIN
MX2009000842A (en) * 2006-08-02 2009-02-03 Starck H C Gmbh Recovery of molybdenum from molybdenum bearing sulfide materials by bioleaching in the presence of iron.
ZA200708340B (en) * 2006-10-27 2008-10-29 Biosigma Sa Process to increase the bioleaching speed of ores or concentrates of sulfide metal species, by means of continuous inoculation with leaching solution that contains isolated microorganisms, with or without the presence of native microorganisms

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Debabrata Pradhan等.Effect of Ni2+, V4+ and Mo6+ concentration on iron oxidation by Acidithiobacillus ferrooxidans.<Korean J. Chem. Eng.>.2009,第26卷(第3期),736-741. *
Tsuyoshi Sugio等.Molecular diversity of cytochrome oxidase among Acidithiobacillus ferrooxidans strains resistant to molybdenum, mercury, sulfite and 2,4-dinitrophenol.<Hydrometallurgy>.2003,第71卷159–164. *
郑英等.铀钼共生矿的细菌浸出试验研究.《湿法冶金》.2007,第26卷(第2期),75－79. *

Also Published As

Publication number Publication date
CN102174425A (en) 2011-09-07

Similar Documents

Publication Publication Date Title
CN102174425B (en) Acidithiobacillus and application thereof
CN103993171B (en) A kind of method of adding nonionogenic tenside promotion chalcopyrite Bioleaching
CN101792728B (en) Method for leaching metal from metallic ores and special strain in same
CN105734285B (en) A kind of method for strengthening zincblende Microorganism Leaching
CN104877933A (en) Bacterial strains capable of being used for preparing acidophilic iron oxidizing microbial compound agent and application thereof
CN104862250A (en) Acidophilic iron-oxidizing composite microbial agent and preparation method thereof
Khaleque et al. Chloride ion tolerance and pyrite bioleaching capabilities of pure and mixed halotolerant, acidophilic iron-and sulfur-oxidizing cultures
CN103131650B (en) Eosino-thiobacillus and application thereof to copper pyrites leaching
Liu et al. Isolation of Leptospirillum ferriphilum by single-layered solid medium
CN103667131B (en) A kind of method and special strain therefore thereof improving metallic ore leaching rate
CN101016583A (en) Method of eliminating passivation film for chalcopyrite at lixiviating course by sulfur oxidizing bacteria
CN106635854A (en) Oligographic bacterium composition and application of oligographic bacterium composition in inhibition on generation of jarosite in biological leaching
CN104531990A (en) Biological gold extraction process of antimony-bearing complex refractory gold ore and microorganism used in same
WO2023072310A1 (en) Acidomyces acidothermus strain and use thereof in leaching of copper-containing pollutant of waste circuit boards
CN105132316A (en) Selenium-addicted microbe Wautersiella enshiensis YLK-1 and application thereof
CN102618472A (en) Method for breeding and separating high arsenic oxidizing bacteria
CN105670965B (en) Strain with iron reduction capacity and application thereof
CN105400718B (en) One plant of nitrate relies on ferrous oxidation bacterial strain and its application
Styriakova et al. Nutrients enhancing the bacterial iron dissolution in the processing of feldspar raw materials
CN103540519B (en) Double-layer flat plate and preparation method thereof
CN105803194B (en) A kind of method that arsenic-bearing refractory gold ore microbe-preoxidation gold is carried out using high arsenic ion tolerance leaching microbacteria
CN105199990A (en) Autotrophic and heterotrophic compound ore-leaching flora FIM-Z4 and application thereof
Zhou et al. Isolation and characterization of Acidithiobacillus caldus from several typical environments in China
CN107699511B (en) Thermophilic thiobacillus and culture medium and culture method thereof
CN107287128B (en) Leptospira ferriphila BYL with high copper resistance and high iron resistance and application thereof

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C53 Correction of patent for invention or patent application
CB03 Change of inventor or designer information

Inventor after: Meng Yunsheng

Inventor after: Zheng Ying

Inventor after: Fan Baotuan

Inventor after: Zhou Lei

Inventor after: Cheng Hao

Inventor after: Liu Hui

Inventor after: Shi Liuyin

Inventor before: Meng Yunsheng

Inventor before: Zheng Ying

Inventor before: Zhou Lei

Inventor before: Cheng Hao

Inventor before: Liu Hui

Inventor before: Shi Liuyin

COR Change of bibliographic data

Free format text: CORRECT: INVENTOR; FROM: MENG YUNSHENG ZHENG YING ZHOU LEI CHENG HAO LIU HUI SHI LIUYIN TO: MENG YUNSHENG ZHENG YING FAN BAOTUAN ZHOU LEI CHENG HAO LIU HUI SHI LIUYIN

C14 Grant of patent or utility model
GR01 Patent grant