CN115216627B - Application method of acid-resistant thiobacillus - Google Patents
Application method of acid-resistant thiobacillus Download PDFInfo
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- CN115216627B CN115216627B CN202110427739.0A CN202110427739A CN115216627B CN 115216627 B CN115216627 B CN 115216627B CN 202110427739 A CN202110427739 A CN 202110427739A CN 115216627 B CN115216627 B CN 115216627B
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- 241000605118 Thiobacillus Species 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000002253 acid Substances 0.000 title claims abstract description 10
- 230000001580 bacterial effect Effects 0.000 claims description 22
- 241000894006 Bacteria Species 0.000 claims description 15
- 241000605222 Acidithiobacillus ferrooxidans Species 0.000 claims description 7
- 230000001651 autotrophic effect Effects 0.000 claims description 6
- 230000003287 optical effect Effects 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims 2
- UQSXHKLRYXJYBZ-UHFFFAOYSA-N Iron oxide Chemical compound [Fe]=O UQSXHKLRYXJYBZ-UHFFFAOYSA-N 0.000 abstract description 14
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract description 5
- 229910052770 Uranium Inorganic materials 0.000 abstract description 5
- 238000002386 leaching Methods 0.000 abstract description 5
- JFALSRSLKYAFGM-UHFFFAOYSA-N uranium(0) Chemical compound [U] JFALSRSLKYAFGM-UHFFFAOYSA-N 0.000 abstract description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 abstract description 4
- 229910052802 copper Inorganic materials 0.000 abstract description 4
- 239000010949 copper Substances 0.000 abstract description 4
- 229910052751 metal Inorganic materials 0.000 abstract description 4
- 239000002184 metal Substances 0.000 abstract description 4
- 150000002739 metals Chemical class 0.000 abstract description 4
- 230000010718 Oxidation Activity Effects 0.000 abstract description 2
- 229910052500 inorganic mineral Inorganic materials 0.000 abstract description 2
- 239000011707 mineral Substances 0.000 abstract description 2
- 229910052742 iron Inorganic materials 0.000 abstract 1
- 150000003568 thioethers Chemical class 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 21
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 12
- 230000000694 effects Effects 0.000 description 4
- 230000033116 oxidation-reduction process Effects 0.000 description 4
- 239000000203 mixture Substances 0.000 description 3
- 239000012085 test solution Substances 0.000 description 3
- 241000605272 Acidithiobacillus thiooxidans Species 0.000 description 2
- VTLYFUHAOXGGBS-UHFFFAOYSA-N Fe3+ Chemical compound [Fe+3] VTLYFUHAOXGGBS-UHFFFAOYSA-N 0.000 description 2
- UCKMPCXJQFINFW-UHFFFAOYSA-N Sulphide Chemical compound [S-2] UCKMPCXJQFINFW-UHFFFAOYSA-N 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000589925 Leptospirillum Species 0.000 description 1
- 241000589921 Leptospirillum ferrooxidans Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000009854 hydrometallurgy Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
- C22B3/00—Extraction of metal compounds from ores or concentrates by wet processes
- C22B3/18—Extraction of metal compounds from ores or concentrates by wet processes with the aid of microorganisms or enzymes, e.g. bacteria or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
- C22B15/00—Obtaining copper
- C22B15/0063—Hydrometallurgy
- C22B15/0065—Leaching or slurrying
- C22B15/0067—Leaching or slurrying with acids or salts thereof
- C22B15/0071—Leaching or slurrying with acids or salts thereof containing sulfur
-
- C—CHEMISTRY; METALLURGY
- C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
- C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
- C22B60/00—Obtaining metals of atomic number 87 or higher, i.e. radioactive metals
- C22B60/02—Obtaining thorium, uranium, or other actinides
- C22B60/0204—Obtaining thorium, uranium, or other actinides obtaining uranium
- C22B60/0217—Obtaining thorium, uranium, or other actinides obtaining uranium by wet processes
- C22B60/0221—Obtaining thorium, uranium, or other actinides obtaining uranium by wet processes by leaching
- C22B60/0226—Obtaining thorium, uranium, or other actinides obtaining uranium by wet processes by leaching using acidic solutions or liquors
- C22B60/0234—Obtaining thorium, uranium, or other actinides obtaining uranium by wet processes by leaching using acidic solutions or liquors sulfurated ion as active agent
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P10/00—Technologies related to metal processing
- Y02P10/20—Recycling
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- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
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- Metallurgy (AREA)
- Mechanical Engineering (AREA)
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- Manufacturing & Machinery (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Geology (AREA)
- Environmental & Geological Engineering (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Aiming at the problems of low oxidation activity, low growth and propagation speed and the like of the existing mineral leaching strain in a high-acid environment, the invention provides an application method of acid-resistant thiobacillus, and the strain can be used in H 2 SO 4 The growth and propagation speed is high when the concentration is 40g/L, and the ferrous oxide speed can reach 1 g/L.d. The strain can be used for recovering metals such as uranium, copper and the like from ores containing iron or sulfides.
Description
Technical Field
The invention belongs to the technical field of hydrometallurgy, and particularly relates to an acid-resistant thiobacillus application method.
Background
Bioleaching is the process of leaching useful metals, such as uranium, copper, etc., from ores by using certain bacteria or metabolites thereof, and the technology has the advantages of full utilization of resources, low production cost, no environmental pollution basically, etc.
The existing commonly used mineral leaching microorganisms mainly comprise acidophilic autotrophic bacteria including ThiobaciLLus ferrooxidans (thiobacillus ferrooxidans), thiobaciLLus thiooxidans (thiobacillus thiooxidans), ferrobaciLLus ferrooxidans (ferric iron oxide rod bacteria), leptospiriLLum ferrooxidans (ferric iron oxide leptospirillum) and the like, the pH of the bacteria suitable for survival is 2.0-3.5, when the pH of an environment medium is in the range, the bacterial growth and propagation speed is high, the oxidation activity is high, and the ferrous oxide rate can reach 9 g/L.d. Along with the increase of the sulfuric acid concentration in the system, the ferrous oxide speed is reduced, when the sulfuric acid concentration is 20g/L, the bacterial ferrous oxide speed is 0.5 g/L.d,
when the concentration of sulfuric acid in mine adsorption tail liquid is high, the speed of bacterial ferrous oxide is low, so that the leaching effect of uranium or other metals is affected.
Disclosure of Invention
The invention aims at: thiobacillus capable of growing in a high acid environment is provided.
The technical scheme of the invention is as follows: an application method of acid-fast thiobacillus comprises the following steps:
s1: preparing a solution comprising H 2 SO 4 40g/L，FeSO 4 ·7H 2 O 15g/L，(NH 4 ) 2 SO 4 3g/L；
S2: placing the solution prepared in the step S1 into a conical flask;
s3: taking bacterial SWU3 culture solution in a conical flask in the step S2, and uniformly mixing;
s4: the conical flask obtained in S3 was placed in a thermostatted shaker.
In S2, 80ml of the solution prepared in S1 is placed in a conical flask.
In S2, 80ml of the solution prepared in S1 was placed in a 200ml conical flask.
In S3, 20ml of bacterial SWU3 broth was taken in the Erlenmeyer flask in S2.
In S4, the conical flask obtained in S3 is placed in a constant temperature shaking table at 30 ℃.
In the step S4, the conical flask obtained in the step S3 is placed in a constant temperature shaking table at 30 ℃ and the rotating speed is 120r/min.
In S3, the bacterial SWU3 is gram negative.
In the S3, the bacterial SWU3 has small and rod-shaped bacterial cells under an optical microscope, can move and has the size of 0.3-0.5 mu m multiplied by 1.2-1.9 mu m.
In the step S3, the bacterial SWU3 is acidophilic autotrophic bacteria.
In the step S3, the suitable growth temperature of the bacterial SWU3 is 20-35 ℃, and the growth pH is less than 2.0.
The invention has the remarkable effects that: the thiobacillus (Acidithiobacillus ferrooxidans SWU 3) has strong high acid resistance, can grow well in the environment with the sulfuric acid concentration of 40g/L, and the ferrous oxide rate can reach 1 g/L.d. The strain can be widely applied to uranium ore and copper ore leaching.
Detailed Description
The present invention will be further described with reference to examples, but the scope of the present invention is not limited to the examples.
The invention aims to provide a thiobacillus which can grow in a high-acid environment and has higher activity and oxidation efficiency and an application method thereof.
The chemolithotrophic bacterium (SWU 3) of the invention has been deposited in China general microbiological culture Collection center, address: the collection number is CGMCC No.17692 and the collection name is Acidithiobacillus ferrooxidans SWU of the national academy of sciences of China, national institute of microbiology, north Chen West Lu No.1, no. 3 of the Chaoyang district of Beijing;
the classification name is suggested as thiobacillus ferrooxidans Thiobacillus ferrooxidans.
The autotrophic thiobacillus (SWU 3) is gram negative, has small thallus and rod shape under optical microscope, and can move with size of 0.3-0.5 μm×1.2-1.9 μm.
The thiobacillus is a typical acidophilic autotrophic bacteria by oxidizing Fe 2+ Or sulfide to obtain energy growth, the product being Fe 3+ Sulfuric acid, sulfate, using CO 2 Is a carbon source, the proper growth temperature is 20-35 ℃, and the growth pH is<2.0。
The autotrophic thiobacillus has strong high acid resistance, can grow well in the environment with the sulfuric acid concentration of 40g/L, and the ferrous oxide rate can reach 1 g/L.d.
The chemolithotrophic thiobacillus (SWU 3) of the invention can be used for preparing the feed from Fe-containing feed 2+ Or recovering metals such as uranium and copper from sulfide ores
Comparative example
The main composition of the test solution is as follows: h 2 SO 4 40g/L，FeSO 4 ·7H 2 O 15g/L，(NH 4 ) 2 SO 4 3g/L. The conical flask, the measuring cylinder and the like are sterilized.
The conventional method is used in laboratory at ρ (H 2 SO 4 ) 2g/L of strain M1, comprising the following steps:
(1) Placing 80ml of the solution into a 200ml conical flask;
(2) Taking 20ml of bacterial M1 culture solution in the conical flask in the step (1), and uniformly mixing;
(3) Placing the conical flask obtained in the step (2) in a constant temperature shaking table at 30 ℃ at a rotating speed of 120r/min, measuring the oxidation-reduction potential of the solution every day, and observing bacteria in the solution under a microscope periodically.
The results show that: after 10 days of incubation, the redox potential of the solution in the Erlenmeyer flask was 400mv, and the number of bacteria observed under the microscope was small, indicating that the bacterial activity was low.
Example 1
The main composition of the test solution is as follows: h 2 SO 4 40g/L，FeSO 4 ·7H 2 O 15g/L，(NH 4 ) 2 SO 4 3g/L. The conical flask, the measuring cylinder and the like are sterilized.
The strain SWU3 of the invention was used in the laboratory as follows:
(1) Placing 80ml of the solution into a 200ml conical flask;
(2) Taking 20ml of bacterial SWU3 culture solution in the conical flask in the step (1), and uniformly mixing;
(3) Placing the conical flask obtained in the step (2) in a constant temperature shaking table at 30 ℃ and rotating at 120r/min, measuring the oxidation-reduction potential of the solution, and observing bacteria in the solution under a microscope at regular intervals.
The results show that: after 3 days of culture, the redox potential of the solution in the conical flask increased to 560mv, and the number of bacteria was observed under a microscope, which indicates that the activity of the bacteria was high.
Example 2
The main composition of the test solution is as follows: h 2 SO 4 40g/L，FeSO 4 ·7H 2 O 15g/L，(NH 4 ) 2 SO 4 3g/L. All of the glass container, measuring cylinder, etc. are killedBacteria.
The strain SWU3 of the invention was used in the laboratory as follows:
(1) Putting 1600ml of the solution into a wide-mouth bottle;
(2) Taking 400ml of bacterial SWU3 culture solution in the wide-mouth bottle in the step (1), and uniformly mixing;
(3) Placing a heating rod in the wide-mouth bottle obtained in the step (2), keeping the temperature of the solution at 30 ℃, measuring the oxidation-reduction potential of the solution every day, and observing bacteria in the solution under a microscope at regular intervals.
The results show that: after 3 days of culture, the oxidation-reduction potential of the solution in the jar was raised to 520mv, and the number of bacteria was observed under a microscope, indicating high bacterial activity.
Claims (10)
1. An application method of acid-resistant thiobacillus is characterized in that: the method comprises the following steps:
s1: preparing a solution comprising H 2 SO 4 40g/L，FeSO 4 ·7H 2 O 15g/L，(NH 4 ) 2 SO 4 3g/L；
S2: placing the solution prepared in the step S1 into a conical flask;
s3: taking bacterial SWU3 culture solution in a conical flask in the step S2, and uniformly mixing; the preservation number is CGMCC No.17692 and the preservation name is Acidithiobacillus ferrooxidans SWU;
s4: the conical flask obtained in S3 was placed in a thermostatted shaker.
2. The method for applying acid-fast thiobacillus according to claim 1, wherein: in S2, 80ml of the solution prepared in S1 is placed in a conical flask.
3. The method for applying acid-fast thiobacillus according to claim 2, wherein: in S2, 80ml of the solution prepared in S1 was placed in a 200ml conical flask.
4. The method for applying acid-fast thiobacillus according to claim 1, wherein: in S3, 20ml of bacterial SWU3 broth was taken in the Erlenmeyer flask in S2.
5. The method for applying acid-fast thiobacillus according to claim 1, wherein: in S4, the conical flask obtained in S3 is placed in a constant temperature shaking table at 30 ℃.
6. The method for applying acid-fast thiobacillus according to claim 5, wherein: in the step S4, the conical flask obtained in the step S3 is placed in a constant temperature shaking table at 30 ℃ and the rotating speed is 120r/min.
7. The method for applying acid-fast thiobacillus according to claim 1, wherein: in S3, the bacterial SWU3 is gram negative.
8. The method for applying acid-fast thiobacillus according to claim 7, wherein: in the S3, the bacterial SWU3 has small and rod-shaped bacterial cells under an optical microscope, can move and has the size of 0.3-0.5 mu m multiplied by 1.2-1.9 mu m.
9. The method for applying acid-fast thiobacillus according to claim 8, wherein: in the step S3, the bacterial SWU3 is acidophilic autotrophic bacteria.
10. The method for applying acid-fast thiobacillus according to claim 9, wherein: in the step S3, the suitable growth temperature of the bacterial SWU3 is 20-35 ℃, and the growth pH is less than 2.0.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110427739.0A CN115216627B (en) | 2021-04-21 | 2021-04-21 | Application method of acid-resistant thiobacillus |
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| CN202110427739.0A CN115216627B (en) | 2021-04-21 | 2021-04-21 | Application method of acid-resistant thiobacillus |
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| CN115216627A CN115216627A (en) | 2022-10-21 |
| CN115216627B true CN115216627B (en) | 2024-03-19 |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4888293A (en) * | 1987-07-10 | 1989-12-19 | Giant Bay Biotech Inc. | Adapting bacteria to low pH and high arsenic concentration for use in oxidizing sulfide ores |
| RU2349641C1 (en) * | 2007-08-06 | 2009-03-20 | Закрытое акционерное общество научно-производственное предприятие "Биомедхим" (ЗАО НПП "Биомедхим") | Bacterial strain acidithiobacillus ferrooxidans from bioleaching copper from wastes of enriching sulfide ore |
| CN101891166A (en) * | 2009-12-01 | 2010-11-24 | 西部矿业股份有限公司 | Method for leaching low-grade phosphorite with mixed bacillus of thiobacillus thioxidans and thiobacillus ferrooxidans |
| CN102174425A (en) * | 2010-12-23 | 2011-09-07 | 核工业北京化工冶金研究院 | Acidithiobacillus and application thereof |
| CN111808773A (en) * | 2020-07-22 | 2020-10-23 | 东华理工大学 | Acidithiobacillus ferrooxidans and application thereof and ore leaching method |
-
2021
- 2021-04-21 CN CN202110427739.0A patent/CN115216627B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4888293A (en) * | 1987-07-10 | 1989-12-19 | Giant Bay Biotech Inc. | Adapting bacteria to low pH and high arsenic concentration for use in oxidizing sulfide ores |
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| CN101891166A (en) * | 2009-12-01 | 2010-11-24 | 西部矿业股份有限公司 | Method for leaching low-grade phosphorite with mixed bacillus of thiobacillus thioxidans and thiobacillus ferrooxidans |
| CN102174425A (en) * | 2010-12-23 | 2011-09-07 | 核工业北京化工冶金研究院 | Acidithiobacillus and application thereof |
| CN111808773A (en) * | 2020-07-22 | 2020-10-23 | 东华理工大学 | Acidithiobacillus ferrooxidans and application thereof and ore leaching method |
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| Title |
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| 一株浸矿细菌的分离及其对低品位硫化镍铜矿中镍的浸出;熊学权;季秀玲;魏云林;林连兵;;昆明理工大学学报(理工版)(第02期);26-32 * |
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| 适应铀矿石的耐极低pH值氧化亚铁硫杆菌的诱变育种;刘亚洁, 李江, 陈功新, 刘艳;矿产综合利用(第02期);22-27 * |
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