CN115216418A - Application method of low-temperature-resistant thiobacillus - Google Patents

Application method of low-temperature-resistant thiobacillus Download PDF

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Publication number
CN115216418A
CN115216418A CN202110427834.0A CN202110427834A CN115216418A CN 115216418 A CN115216418 A CN 115216418A CN 202110427834 A CN202110427834 A CN 202110427834A CN 115216418 A CN115216418 A CN 115216418A
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thiobacillus
solution
conical flask
low temperature
temperature resistant
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孟运生
张静敏
刘辉
郑英
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Beijing Research Institute of Chemical Engineering and Metallurgy of CNNC
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Beijing Research Institute of Chemical Engineering and Metallurgy of CNNC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F3/00—Biological treatment of water, waste water, or sewage
    • C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
    • C—CHEMISTRY; METALLURGY
    • C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
    • C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
    • C22B15/00—Obtaining copper
    • C22B15/0063—Hydrometallurgy
    • C22B15/0065—Leaching or slurrying
    • C—CHEMISTRY; METALLURGY
    • C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
    • C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
    • C22B3/00—Extraction of metal compounds from ores or concentrates by wet processes
    • C22B3/18—Extraction of metal compounds from ores or concentrates by wet processes with the aid of microorganisms or enzymes, e.g. bacteria or algae
    • C—CHEMISTRY; METALLURGY
    • C22—METALLURGY; FERROUS OR NON-FERROUS ALLOYS; TREATMENT OF ALLOYS OR NON-FERROUS METALS
    • C22B—PRODUCTION AND REFINING OF METALS; PRETREATMENT OF RAW MATERIALS
    • C22B60/00—Obtaining metals of atomic number 87 or higher, i.e. radioactive metals
    • C22B60/02—Obtaining thorium, uranium, or other actinides
    • C22B60/0204—Obtaining thorium, uranium, or other actinides obtaining uranium
    • C22B60/0217—Obtaining thorium, uranium, or other actinides obtaining uranium by wet processes
    • C22B60/0221—Obtaining thorium, uranium, or other actinides obtaining uranium by wet processes by leaching
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P10/00—Technologies related to metal processing
    • Y02P10/20—Recycling

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  • Environmental & Geological Engineering (AREA)
  • Health & Medical Sciences (AREA)
  • Metallurgy (AREA)
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Abstract

Aiming at the problems of low oxidation activity, low growth and propagation speed and the like of the existing leaching strain in a low-temperature environment, the invention provides a strain of low-temperature resistant bacteria, the strain can grow and propagate at a high speed at 10 ℃, and the ferrous oxide speed can reach 1.27 g/L.d. The strain can be used for recovering metals such as uranium, copper and the like from iron-containing or sulfide-containing ores, and can also be used for sewage treatment.

Description

Application method of low-temperature-resistant thiobacillus
Technical Field
The invention belongs to the technical field of hydrometallurgy, and particularly relates to low-temperature-resistant chemoautotrophic thiobacillus and an application method thereof.
Background
Some bacteria or their metabolites can leach useful metals, such as uranium, copper, etc., from ores, and compared with conventional chemical leaching, the technology has the advantages of full utilization of resources, low production cost, basically no environmental pollution, etc.
Bacteria used for leaching ores are classified into three categories according to their optimal growth temperatures, namely mesophilic (25-40 ℃), moderate thermophilic (45-50 ℃) and thermophilic (55-80 ℃). At present, mesophilic bacteria are often used in scientific research experiments and industrial application, and the bacteria have high growth and reproduction speed and high oxidation activity at the temperature of 20-35 ℃. Taking the frequently used Af bacteria as an example, the optimal growth temperature range is 28-35 ℃, when the environmental temperature is in the optimal range, the growth and propagation speed of the bacteria is fast, the ferrous oxide speed can reach 9 g/L.d, the ferrous oxide speed is reduced along with the reduction of the temperature, when the temperature is reduced to about 10 ℃, the bacterial activity is extremely low, and the ferrous oxide speed is less than 0.1 g/L.d, even lower.
When the mine adopting the bacterial leaching technology is in winter or in a severe cold area, the production efficiency is low. The adverse effect of low temperature on microorganisms also limits the rate of biochemical treatment of wastewater.
Disclosure of Invention
The invention aims to: provides a thiobacillus which can grow in low-temperature environment.
The technical scheme of the invention is as follows: a method for applying low temperature resistant thiobacillus comprises the following steps:
s1: preparing an experimental solution comprising FeSO 4 ·7H 2 O 45g/L，(NH 4 ) 2 SO 4 3g/L，pH 2.0；
S2: placing the solution prepared in the S1 in a conical flask;
s3: putting bacterial SWU2 culture solution into a conical flask in S2, and mixing uniformly
S4: and (4) placing the conical flask obtained in the step (S3) in a constant-temperature shaking table.
In the S1, 80ml of the solution is placed in an erlenmeyer flask.
In the S1, 80ml of the solution is placed in a 200ml conical flask.
In S3, 20ml of bacterial SWU2 culture was taken in an Erlenmeyer flask in S2.
In the S1, an experimental solution is prepared by using a conical flask and a measuring cylinder, and the conical flask and the measuring cylinder are sterilized.
In the step S4, the conical flask obtained in the step S3 is placed in a constant temperature shaking table at the temperature of 10 ℃,
in S4, the rotating speed is 120r/min.
In S3, the bacterium SWU2 is an acidophilic autotrophic bacterium.
In the S3, the growth temperature of bacteria SWU2 is 10-30 ℃; the growth pH is 1.8-2.5.
In S3, the size of the bacteria SWU2 is 0.4-0.6 μm multiplied by 2-2.5 μm.
The invention has the remarkable effects that: the thiobacillus (Acidithiobacillus ferrooxidans SWU 2) has strong low temperature resistance, can still grow well at the temperature of 10 ℃, and has the ferrous oxidation rate of 1.27 g/L.d. The strain can be widely applied to leaching of uranium ores and copper ores, and can also be widely applied to environmental protection, such as sewage treatment and the like.
Detailed Description
The present invention is further illustrated by the following examples, which are not intended to limit the scope of the invention.
The invention aims to provide thiobacillus which can grow in a low-temperature environment and has higher activity and oxidation efficiency and an application method thereof.
The chemoautotrophic bacteria (SWU 2) disclosed by the invention are preserved in China general microbiological culture Collection center on 23.5.2019, and the addresses are as follows: no. 3 of Xilu No.1 of Beijing, chaoyang, china academy of sciences microbiological research, with the collection number of CGMCC No.17691 and the collection name of Acidithiobacillus ferrooxidans SWU2.
Suggested taxonomic nomenclature Thiobacillus ferrooxidans, thiobacillus ferrooxidans
The chemoautotrophic thiobacillus (SWU 2) is gram-negative, has small and rod-shaped thallus under an optical microscope, can move, and has a size of 0.4-0.6 μm × 2-2.5 μm.
The Thiobacillus species is typically an acidophilic autotrophic species, by oxidation of Fe 2+ And sulfide to obtain energy growth, the product being Fe 3+ Sulfur, sulfurAcids, sulfates; by using CO 2 Is a carbon source; the growth temperature is 10-30 ℃; the growth pH is 1.8-2.5.
The chemoautotrophic thiobacillus has strong low-temperature resistance, can still grow well at the temperature of 10 ℃, and has the ferrous oxide rate of 1.27 g/L.d.
The chemoautotrophic thiobacillus (SWU 2) of the invention can be used for removing Fe 2+ Or recovering metals such as uranium and copper from sulfide ores
Comparative example
The test solution consisted essentially of: feSO 4 ·7H 2 O 45g/L，(NH 4 ) 2 SO 4 3g/L, pH 2.0, the conical flask, measuring cylinder, etc. used all need to be sterilized.
The method adopts a commonly used strain M1 with a growth temperature of 30 ℃ in a laboratory, and comprises the following specific steps:
(1) Putting 80ml of the solution into a 200ml conical flask;
(2) Taking 20ml of bacterial M1 culture solution to be put into the conical flask in the step (1) and uniformly mixing;
(3) And (3) placing the conical flask obtained in the step (2) in a constant-temperature shaking table at 10 ℃, rotating at 120r/min, observing the color of the solution every day, measuring the oxidation-reduction potential of the solution, and regularly observing bacteria in the solution under a microscope.
The results show that: after 10 days of culture, the color of the solution in the conical flask changes slightly and is still light green, the oxidation-reduction potential of the solution is 400mv, and the number of bacteria observed under a microscope is small, which indicates that the activity of the bacteria is low.
Example 1
The test solution consisted essentially of: feSO 4 ·7H 2 O 45g/L，(NH 4 ) 2 SO 4 3g/L, pH 2.0, the conical flask, measuring cylinder, etc. used all need to be sterilized.
The strain SWU2 is adopted in a laboratory, and the specific steps are as follows:
(1) Putting 80ml of the solution into a 200ml conical flask;
(2) Taking 20ml of bacterial SWU2 culture solution to be uniformly mixed in the conical flask in the step (1);
(3) And (3) placing the conical flask obtained in the step (2) in a constant-temperature shaking table at 10 ℃, rotating at 120r/min, observing the color of the solution every day, measuring the oxidation-reduction potential of the solution, and regularly observing bacteria in the solution under a microscope.
The results show that: after 7 days of culture, the color of the solution in the conical flask is changed from light green to brownish red, the oxidation-reduction potential of the solution is increased to 508mv, and the number of bacteria is observed under a microscope, which indicates that the activity of the bacteria is high.
Example 2
The test solution consisted essentially of: feSO 4 ·7H 2 O 45g/L，(NH 4 ) 2 SO 4 3g/L, pH 2.0, the conical flask, measuring cylinder, etc. used all need to be sterilized.
The strain SWU2 is adopted in a laboratory, and the specific steps are as follows:
(1) Putting 80ml of the solution into a 200ml conical flask;
(2) Taking 20ml of bacterial SWU2 culture solution to be placed in the conical flask in the step (1), and uniformly mixing;
(3) And (3) placing the conical flask obtained in the step (2) in a constant-temperature shaking table at the temperature of 20 ℃, rotating at the speed of 120r/min, observing the color of the solution every day, measuring the oxidation-reduction potential of the solution, and regularly observing bacteria in the solution under a microscope.
The results show that: after 7 days of culture, the color of the solution in the conical flask is changed from light green to brownish red, the oxidation-reduction potential of the solution is increased to 508mv, and the number of bacteria is observed under a microscope, which indicates that the activity of the bacteria is high.

Claims (10)

1. The application method of the low temperature resistant thiobacillus is characterized by comprising the following steps: the method comprises the following steps:
s1: preparing experimental solution including FeSO 4 ·7H 2 O 45g/L，(NH 4 ) 2 SO 4 3g/L，pH 2.0；
S2: putting the solution prepared in the step S1 into a conical flask;
s3: putting bacterial SWU2 culture solution into a conical flask in S2, and mixing uniformly
S4: and (4) placing the conical flask obtained in the step (S3) in a constant-temperature shaking table.
2. The method for using low temperature resistant Thiobacillus as claimed in claim 1, wherein: in the S1, 80ml of the solution is placed in an erlenmeyer flask.
3. The method for using low temperature resistant Thiobacillus as claimed in claim 2, wherein: in the S1, 80ml of the solution is placed in a 200ml conical flask.
4. The method for using low temperature resistant Thiobacillus as claimed in claim 1, wherein: in S3, 20ml of bacterial SWU2 culture was taken in an Erlenmeyer flask in S2.
5. The method for using low temperature resistant Thiobacillus as claimed in claim 1, wherein: in the step S1, an experimental solution is prepared by using a conical flask and a measuring cylinder, and the conical flask and the measuring cylinder are sterilized.
6. The method for using low temperature resistant Thiobacillus as claimed in claim 1, wherein: in S4, the conical flask obtained in S3 is placed in a constant temperature shaking table at 10 ℃.
7. The method for using low temperature resistant Thiobacillus as claimed in claim 1, wherein: in the S4, the rotating speed is 120r/min.
8. The method for using low temperature resistant Thiobacillus as claimed in claim 1, wherein: in S3, the bacterium SWU2 is an acidophilic autotrophic bacterium.
9. The method for using low temperature resistant Thiobacillus as claimed in claim 1, wherein: in the S3, the growth temperature of bacteria SWU2 is 10-30 ℃; the growth pH is 1.8-2.5.
10. The method for using low temperature resistant Thiobacillus as claimed in claim 1, wherein: in S3, the size of the bacteria SWU2 is 0.4-0.6 μm multiplied by 2-2.5 μm.
CN202110427834.0A 2021-04-21 2021-04-21 Application method of low-temperature-resistant thiobacillus Pending CN115216418A (en)

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Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102174425A (en) * 2010-12-23 2011-09-07 核工业北京化工冶金研究院 Acidithiobacillus and application thereof
CN108913890A (en) * 2018-07-27 2018-11-30 南华大学 Cold-resistant acidophil low temperature quickly aoxidizes Fe in in-situ acid uranium leaching solution2+Method
CN111808773A (en) * 2020-07-22 2020-10-23 东华理工大学 Acidithiobacillus ferrooxidans and application thereof and ore leaching method

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102174425A (en) * 2010-12-23 2011-09-07 核工业北京化工冶金研究院 Acidithiobacillus and application thereof
CN108913890A (en) * 2018-07-27 2018-11-30 南华大学 Cold-resistant acidophil low temperature quickly aoxidizes Fe in in-situ acid uranium leaching solution2+Method
CN111808773A (en) * 2020-07-22 2020-10-23 东华理工大学 Acidithiobacillus ferrooxidans and application thereof and ore leaching method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
袁檬等: "嗜酸性氧化亚铁硫杆菌低温诱变试验研究", 《湖北大学学报( 自然科学版)》 *

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