CN102965303A - Breeding method for cobalt leaching bacteria - Google Patents
Breeding method for cobalt leaching bacteria Download PDFInfo
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- CN102965303A CN102965303A CN2012104169556A CN201210416955A CN102965303A CN 102965303 A CN102965303 A CN 102965303A CN 2012104169556 A CN2012104169556 A CN 2012104169556A CN 201210416955 A CN201210416955 A CN 201210416955A CN 102965303 A CN102965303 A CN 102965303A
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Abstract
A breeding method for cobalt leaching bacteria belongs to the technical field of biological metallurgy. The method provided by the invention comprises the following steps: collecting acidophilic leaching bacterial liquid in pit water in copper sulphide cobalt mine; inoculating the acidophilic leaching bacterial liquid to obtain cultured bacteria, and diluting to obtain an inoculation bacterial liquid; domesticating the inoculated bacterial fluid to obtain a domesticated bacterial liquid; preparing the domesticated bacterial liquid into a breeding bacterial solution, and then preparing into an iron-free breeding solution; adding cobalt sulphide ore into the iron-free breeding solution for iron-free breeding; repeating the iron-free breeding; standing the iron-free breeding solution until solid precipitation, and then filtering to remove the liquid; and placing the obtained bacteria into a 9K culture medium to prepare a bacterial liquid with bacterial concentration of 108 cell / mL, so as to obtain breeding cobalt leaching bacteria. The cobalt leaching bacteria obtained by the invention is an high-activity and high-benefit leaching bacteria adapting to deposit environment, and has important economic and strategic significance for further development and utilization of cobalt resource and increase of cobalt yield.
Description
Technical field
The invention belongs to the biological metallurgy technical field, particularly a kind of selection that soaks the cobalt bacterium.
Background technology
Along with the development of society and the day by day poorness of mineral wealth, at rich ore, easily processing ore resources day by day reduces, environmental requirement improves constantly, today that modern industry and development in science and technology grow with each passing day to the demand of metal, low based on biological metallurgy technology production cost, the processing ore amount is large, be particularly suited for processing low-grade mineral resource, and can carry out comprehensive reutilization to various metals, environmental pollution is little simultaneously, compare with other mineral products treatment technologies, the utilization of biological metallurgy technology not only has obvious economic benefit, and have significant social benefit and environmental benefit, so the Bioleaching technology is now and will play the part of more and more important role future soon.Be accompanied by the development of bioleaching technology, ore-leaching bacteria therein role is more and more important, and different kinds constantly is separated with the microorganism with difference in functionality.
At China's cobalt resource wretched insufficiency, the cobalt concentrate reserves seldom, most forms with Coexisting Elements are present in the middle of the mineral ores such as nickel, copper, iron, and grade is low, only is 0.02% according to verifying China's cobalt resource average grade.Along with the continuous exploitation of rich cobalt ore, cobalt resource constantly reduces, and it is domestic now to the demand of cobalt metal to lean on a large amount of import cobalts to satisfy; Developing a kind of effective cobalt ore acquisition method is present urgent problem.
Summary of the invention
The invention provides a kind of selection that soaks the cobalt bacterium, the characteristics of utilizing mixed bacterium to have complementary advantages by mixed bacterium is repeatedly tamed, obtain to have the ore-leaching bacteria that well soaks the ore deposit ability.The bacterium that the present invention adopts is all in the center preservation of Chinese Typical Representative culture collection, the little spirillum SYZC-023 of iron protoxide Leptospirillum ferrooxidans SYZC-023, and deposit number is CCTCC NO:M2011202; Have a liking for the ferrous thiobacillus ZBYCO-12-1 of acid oxidase Acidithiobacillus ferrooxidans ZBYCO-12-1, deposit number is CCTCC NO:M2011203; Acidithiobacillus thiooxidans ZCO-101 Acidithiobacillus thiooxidans ZCO-101, deposit number is CCTCC NO:M2011204; Preservation date is on June 20th, 2011, address: China. Wuhan. and Wuhan University.
Method of the present invention is carried out according to the following steps:
1, gather in the pit water on cobalt ore mountain and soak ore deposit bacterium bacterium liquid, described to soak the ore deposit bacterium be the mixed bacterium that contains the little spirillum of iron protoxide, has a liking for the ferrous thiobacillus of acid oxidase and Acidithiobacillus thiooxidans;
2, will soak ore deposit bacterium bacterium liquid at the 9K inoculation of medium, inoculation is soaked ore deposit bacterium bacterium liquid and is accounted for 20% of cumulative volume, regulate pH value to 1.2 ~ 2.5, in airbath constant temperature oscillation case, under temperature 60 C and rotating speed 190rpm condition, carry out shaking culture one time, when soaking the ore deposit bacteria growing to logarithmic phase, to have a liking for leaching-ore bacteria bacterium liquid takes out and to put into the 9K substratum again and carry out the secondary shaking culture by above-mentioned condition, when soaking the ore deposit bacteria growing to logarithmic phase, will soak ore deposit bacterium bacterium liquid and take out and put into again the 9K substratum and carry out shaking culture three times by above-mentioned condition;
3, be that 0.22 micron filtering with microporous membrane is removed liquid with the ore deposit bacterium bacterium liquid that soaks after three shaking culture with the aperture, bacterium after obtaining to cultivate, will cultivating rear bacterium with the 9K substratum, to be diluted to concentration be 10
8Cell/mL obtains inoculation bacterium liquid;
4, will inoculate bacterium liquid puts into the 9K substratum and makes inoculation solution, wherein inoculate bacterium liquid and account for 5% of cumulative volume, 9K cultivates 95% of fiduciary point cumulative volume, the pH value to 1.2 of adjusting inoculation solution ~ 2.5, adding rose vitriol to concentration of cobalt ions is 1g/L again, then in airbath constant temperature oscillation case, under 44 ~ 60 ℃ of temperature and rotating speed 150 ~ 240rpm condition, once tame, to rise to concentration be 10 to bacterial number in inoculation solution
8When cell/mL is above, and ferrous ion is whole when oxidized in the 9K substratum, bacterium liquid is taken out tame next time, adds rose vitriol to concentration of cobalt ions at every turn when taming and manys 1g/L than last domestication, carries out altogether obtaining to tame bacterium liquid after 35 domestications;
5, will tame and put into the 9K substratum after bacterium liquid takes out and make seed selection solution, wherein tame bacterium liquid and account for 5 ~ 10% of seed selection overall solution volume, 9K cultivates 90 ~ 95% of fiduciary point seed selection overall solution volume, the pH value to 1.2 of adjusting seed selection solution ~ 2.5, then in airbath constant temperature oscillation case, under 44 ~ 60 ℃ of temperature and rotating speed 150 ~ 240rpm condition, carry out seed selection;
6, whole when oxidized when ferrous ion in the seed selection solution, in seed selection solution, add the sulfuration cobalt ore, the weight of sulfuration cobalt ore accounts for 1% of seed selection total solution weight; The quantity growth to 10 of bacterium in seed selection solution
8When cell/mL was above, the seed selection solution of taking-up 1/4 was as bacterium liquid for subsequent use;
7, bacterium liquid for subsequent use is put into iron-free 9K substratum and make breeding of leaching bacteria solution, iron-free 9K cultivates 90 ~ 95% of fiduciary point breeding of leaching bacteria overall solution volume, and all the other are bacterium liquid for subsequent use; Add the sulfuration cobalt ore in breeding of leaching bacteria solution, the weight of sulfuration cobalt ore accounts for 2% of breeding of leaching bacteria total solution weight; Breeding of leaching bacteria in airbath constant temperature oscillation case, is carried out in the pH value to 1.2 of adjusting breeding of leaching bacteria solution ~ 2.5 then under 44 ~ 60 ℃ of temperature and rotating speed 150 ~ 240rpm condition;
8, the quantity growth to 10 of bacterium in breeding of leaching bacteria solution
8When cell/mL is above, the breeding of leaching bacteria solution of taking-up 1/4 is as bacterium liquid for subsequent use, repeat to carry out breeding of leaching bacteria by the mode of step 7, the gross weight that each weight that adds the sulfuration cobalt ore accounts for breeding of leaching bacteria solution Duo 1% than last, and the weight that cobalt ore is vulcanized in last adding accounts for 10% of breeding of leaching bacteria total solution weight; After last breeding of leaching bacteria finishes, to solid precipitation, then partly be 0.22 micron millipore filtration membrane filtration removal liquid with the breeding of leaching bacteria solution left standstill with the aperture with supernatant liquor, the bacterium of acquisition is put into the 9K substratum, and to make bacterial concentration be 10
8The bacterium liquid of cell/mL, what obtain seed selection soaks the cobalt bacterium.
Adopting concentration when regulating the pH value in the aforesaid method is the sulphuric acid soln of 1N.
Above-mentioned sulfuration cobalt ore is corallite, linnaeite, cobaltite, cobalt pyrite or cattierite.
The bacterium that the present invention adopts is the little spirillum of iron protoxide, have a liking for the mixed bacterium that the ferrous thiobacillus of acid oxidase and Acidithiobacillus thiooxidans form, have good oxidation activity under 44 ~ 60 ℃ of conditions, three kinds of bacterium mix the characteristics that have synergy, have complementary advantages; Bacterium is take low price iron and reduced sulfur as the energy, in the culture environment of high ferro low-sulfur, and the little spirillum of iron protoxide and to have a liking for the ferrous thiobacillus of acid oxidase be dominant bacteria, in the culture environment of the low iron of high-sulfur, Acidithiobacillus thiooxidans is dominant bacteria; The cobalt bacterium of soaking that the present invention obtains has the good ore deposit ability of soaking through test, be a kind of high reactivity that adapts to the mineral deposit environment, high benefit soak the ore deposit bacterium, further development and use cobalt resource and increase cobalt output are had important economy and strategic importance, and the suitability for industrialized production of suitable biological metallurgy technology.
Embodiment
The bacterium that the embodiment of the invention adopts is all in the center preservation of Chinese Typical Representative culture collection, the little spirillum SYZC-023 of iron protoxide Leptospirillum ferrooxidans SYZC-023, and deposit number is CCTCC NO:M2011202; Have a liking for the ferrous thiobacillus ZBYCO-12-1 of acid oxidase Acidithiobacillus ferrooxidans ZBYCO-12-1, deposit number is CCTCC NO:M2011203; Acidithiobacillus thiooxidans ZCO-101 Acidithiobacillus thiooxidans ZCO-101, deposit number is CCTCC NO:M2011204; Preservation date is on June 20th, 2011, address: China. Wuhan. and Wuhan University.
Do not soak the ore deposit contrast by taming bacterium and the bacterium of soaking the ore deposit seed selection in the embodiment of the invention, use the leaching yield of the bacterium cobalt that soaks the ore deposit seed selection to improve more than 30%.
Embodiment 1
The sulfuration cobalt ore that adopts is corallite;
Adopting concentration when regulating the pH value is the sulphuric acid soln of 1N;
The preparation method is:
1, gather in the pit water on cobalt ore mountain and soak ore deposit bacterium bacterium liquid, described to soak the ore deposit bacterium be the mixed bacterium that contains the little spirillum of iron protoxide, has a liking for the ferrous thiobacillus of acid oxidase and Acidithiobacillus thiooxidans;
2, will soak ore deposit bacterium bacterium liquid at the 9K inoculation of medium, inoculation is invaded ore deposit bacterium bacterium liquid and is accounted for 20% of cumulative volume, regulate pH value to 1.2, in airbath constant temperature oscillation case, under temperature 60 C and rotating speed 190rpm condition, carry out shaking culture one time, when soaking the ore deposit bacteria growing to logarithmic phase, to soak ore deposit bacterium bacterium liquid takes out and puts into the 9K substratum again and carry out the secondary shaking culture by above-mentioned condition, when soaking the ore deposit bacteria growing to logarithmic phase, will soak ore deposit bacterium bacterium liquid and take out and put into again the 9K substratum and carry out shaking culture three times by above-mentioned condition;
3, be that 0.22 micron filtering with microporous membrane is removed liquid with the ore deposit bacterium bacterium liquid that soaks after three shaking culture with the aperture, bacterium after obtaining to cultivate, will cultivating rear bacterium with the 9K substratum, to be diluted to concentration be 10
8Cell/mL obtains inoculation bacterium liquid;
4, will inoculate bacterium liquid puts into the 9K substratum and makes inoculation solution, wherein inoculate bacterium liquid and account for 5% of cumulative volume, 9K cultivates 95% of fiduciary point cumulative volume, regulate the pH value to 1.2 of inoculation solution, adding rose vitriol to concentration of cobalt ions is 1g/L again, then in airbath constant temperature oscillation case, once tame under temperature 60 C and rotating speed 150rpm condition, to rise to concentration be 10 to bacterial number in inoculation solution
8When cell/mL is above, and ferrous ion is whole when oxidized in the 9K substratum, and bacterium liquid is taken out by tame next time, adds rose vitriol to concentration of cobalt ions at every turn when taming and manys 1g/L than last domestication, carries out altogether obtaining to tame bacterium liquid after 35 domestications;
5, will tame and put into the 9K substratum after bacterium liquid takes out and make seed selection solution, wherein tame bacterium liquid and account for 5% of seed selection overall solution volume, 9K cultivates 95% of fiduciary point seed selection overall solution volume, regulate the pH value to 1.2 of seed selection solution, then in airbath constant temperature oscillation case, under temperature 60 C and rotating speed 150rpm condition, carry out seed selection;
6, whole when oxidized when ferrous ion in the seed selection solution, in seed selection solution, add corallite, corallite accounts for 1% of seed selection total solution weight; The quantity growth to 10 of bacterium in seed selection solution
8When cell/mL was above, the seed selection solution of taking-up 1/4 was as bacterium liquid for subsequent use;
7, bacterium liquid for subsequent use is put into iron-free 9K substratum and make breeding of leaching bacteria solution, iron-free 9K cultivates 95% of fiduciary point breeding of leaching bacteria overall solution volume, and all the other are bacterium liquid for subsequent use; Add corallite in breeding of leaching bacteria solution, the weight of corallite accounts for 2% of breeding of leaching bacteria total solution weight; Regulate the pH value to 1.2 of breeding of leaching bacteria solution, then in airbath constant temperature oscillation case, under temperature 60 C and rotating speed 150rpm condition, carry out breeding of leaching bacteria;
8, the quantity growth to 10 of bacterium in breeding of leaching bacteria solution
8When cell/mL is above, the breeding of leaching bacteria solution of taking-up 1/4 is as bacterium liquid for subsequent use, repeat to carry out breeding of leaching bacteria by the mode of step 7, the gross weight that each weight that adds corallite accounts for breeding of leaching bacteria solution Duos 1% than the last time, and the weight that adds for the last time corallite accounts for 10% of breeding of leaching bacteria total solution weight; After last breeding of leaching bacteria finishes, to solid precipitation, then partly be 0.22 micron millipore filtration membrane filtration removal liquid with the breeding of leaching bacteria solution left standstill with the aperture with supernatant liquor, the bacterium of acquisition is put into the 9K substratum, and to make bacterial concentration be 10
8The bacterium liquid of cell/mL, what obtain seed selection soaks the cobalt bacterium.
The weight that adds corallite accounts in 10% the Bioleaching of breeding of leaching bacteria total solution weight, and the leaching yield of cobalt is 99.3%, and the leaching yield of not taming the bacterium cobalt is 58.2%, and the leaching yield of soaking cobalt bacterium cobalt of seed selection can improve 41.1%.
Embodiment 2
The sulfuration cobalt ore that adopts is linnaeite;
Adopting concentration when regulating the pH value is the sulphuric acid soln of 1N;
The preparation method is:
With seed selection among the embodiment 1 soak the cobalt bacterium as bacterium for subsequent use, bacterium liquid for subsequent use is put into iron-free 9K substratum makes breeding of leaching bacteria solution, iron-free 9K cultivates 94% of fiduciary point breeding of leaching bacteria overall solution volume, all the other are bacterium liquid for subsequent use; Add linnaeite in breeding of leaching bacteria solution, linnaeite accounts for 2% of breeding of leaching bacteria total solution weight; Regulate the pH value to 1.5 of breeding of leaching bacteria solution, then in airbath constant temperature oscillation case, under 44 ℃ of temperature and rotating speed 180rpm condition, carry out breeding of leaching bacteria.
The quantity growth to 10 of bacterium in breeding of leaching bacteria solution
8When cell/mL is above, the breeding of leaching bacteria solution of taking-up 1/4 is as bacterium liquid for subsequent use, repeat to carry out breeding of leaching bacteria by the mode of step 7, the gross weight that each weight that adds linnaeite accounts for breeding of leaching bacteria solution Duos 1% than the last time, and the weight that adds for the last time linnaeite accounts for 10% of breeding of leaching bacteria total solution weight; After last breeding of leaching bacteria finishes, to solid precipitation, then partly be 0.22 micron millipore filtration membrane filtration removal liquid with the breeding of leaching bacteria solution left standstill with the aperture with supernatant liquor, the bacterium of acquisition is put into the 9K substratum, and to make bacterial concentration be 10
8The bacterium liquid of cell/mL, what obtain seed selection soaks the cobalt bacterium.
The weight that adds linnaeite accounts in 10% the Bioleaching of breeding of leaching bacteria total solution weight, and the leaching yield of cobalt is 96.5%, and the leaching yield of not taming the bacterium cobalt is 53.7%, and the leaching yield of soaking cobalt bacterium cobalt of seed selection can improve 42.8%.
Embodiment 3
The sulfuration cobalt ore that adopts is cobaltite;
Adopting concentration when regulating the pH value is the sulphuric acid soln of 1N;
The preparation method is:
With seed selection among the embodiment 2 soak the cobalt bacterium as bacterium for subsequent use, bacterium liquid for subsequent use is put into iron-free 9K substratum makes breeding of leaching bacteria solution, iron-free 9K cultivates 93% of fiduciary point breeding of leaching bacteria overall solution volume, all the other are bacterium liquid for subsequent use; Add cobaltite in breeding of leaching bacteria solution, cobaltite accounts for 2% of breeding of leaching bacteria total solution weight; Regulate the pH value to 2.0 of breeding of leaching bacteria solution, then in airbath constant temperature oscillation case, under temperature 50 C and rotating speed 200rpm condition, carry out breeding of leaching bacteria.
The quantity growth to 10 of bacterium in breeding of leaching bacteria solution
8When cell/mL is above, the breeding of leaching bacteria solution of taking-up 1/4 is as bacterium liquid for subsequent use, repeat to carry out breeding of leaching bacteria by the mode of step 7, the gross weight that each weight that adds cobaltite accounts for breeding of leaching bacteria solution Duos 1% than the last time, and the weight that adds for the last time cobaltite accounts for 10% of breeding of leaching bacteria total solution weight; After last breeding of leaching bacteria finishes, to solid precipitation, then partly be 0.22 micron millipore filtration membrane filtration removal liquid with the breeding of leaching bacteria solution left standstill with the aperture with supernatant liquor, the bacterium of acquisition is put into the 9K substratum, and to make bacterial concentration be 10
8The bacterium liquid of cell/mL, what obtain seed selection soaks the cobalt bacterium.
The weight that adds cobaltite accounts in 10% the Bioleaching of breeding of leaching bacteria total solution weight, and the leaching yield of cobalt is 92.3%, and the leaching yield of not taming the bacterium cobalt is 56.4%, and the leaching yield of soaking cobalt bacterium cobalt of seed selection can improve 35.9%.
Embodiment 4
The sulfuration cobalt ore that adopts is cobalt pyrite;
Adopting concentration when regulating the pH value is the sulphuric acid soln of 1N;
The preparation method is:
With seed selection among the embodiment 3 soak the cobalt bacterium as bacterium for subsequent use, bacterium liquid for subsequent use is put into iron-free 9K substratum makes breeding of leaching bacteria solution, iron-free 9K cultivates 92% of fiduciary point breeding of leaching bacteria overall solution volume, all the other are bacterium liquid for subsequent use; Add cobalt pyrite in breeding of leaching bacteria solution, cobalt pyrite accounts for 2% of breeding of leaching bacteria total solution weight; Regulate the pH value to 2.2 of breeding of leaching bacteria solution, then in airbath constant temperature oscillation case, under 55 ℃ of temperature and rotating speed 220rpm condition, carry out breeding of leaching bacteria.
The quantity growth to 10 of bacterium in breeding of leaching bacteria solution
8When cell/mL is above, the breeding of leaching bacteria solution of taking-up 1/4 is as bacterium liquid for subsequent use, repeat to carry out breeding of leaching bacteria by the mode of step 7, the gross weight that each weight that adds cobalt pyrite accounts for breeding of leaching bacteria solution Duos 1% than the last time, and the weight that adds for the last time cobalt pyrite accounts for 10% of breeding of leaching bacteria total solution weight; After last breeding of leaching bacteria finishes, to solid precipitation, then partly be 0.22 micron millipore filtration membrane filtration removal liquid with the breeding of leaching bacteria solution left standstill with the aperture with supernatant liquor, the bacterium of acquisition is put into the 9K substratum, and to make bacterial concentration be 10
8The bacterium liquid of cell/mL, what obtain seed selection soaks the cobalt bacterium.
The weight that adds cobalt pyrite accounts in 10% the Bioleaching of breeding of leaching bacteria total solution weight, and the leaching yield of cobalt is 91.9%, and the leaching yield of not taming the bacterium cobalt is 50.2%, and the leaching yield of soaking cobalt bacterium cobalt of seed selection can improve 41.4%.
Embodiment 5
The sulfuration cobalt ore that adopts is cattierite;
Adopting concentration when regulating the pH value is the sulphuric acid soln of 1N;
The preparation method is:
With seed selection among the embodiment 4 soak the cobalt bacterium as bacterium for subsequent use, bacterium liquid for subsequent use is put into iron-free 9K substratum makes breeding of leaching bacteria solution, iron-free 9K cultivates 90% of fiduciary point breeding of leaching bacteria overall solution volume, all the other are bacterium liquid for subsequent use; Add cattierite in breeding of leaching bacteria solution, cattierite accounts for 2% of breeding of leaching bacteria total solution weight; Regulate the pH value to 2.5 of breeding of leaching bacteria solution, then in airbath constant temperature oscillation case, under temperature 45 C and rotating speed 240rpm condition, carry out breeding of leaching bacteria.
The quantity growth to 10 of bacterium in breeding of leaching bacteria solution
8When cell/mL is above, the breeding of leaching bacteria solution of taking-up 1/4 is as bacterium liquid for subsequent use, repeat to carry out breeding of leaching bacteria by the mode of step 7, the gross weight that each weight that adds cattierite accounts for breeding of leaching bacteria solution Duos 1% than the last time, and the weight that adds for the last time cattierite accounts for 10% of breeding of leaching bacteria total solution weight; After last breeding of leaching bacteria finishes, to solid precipitation, then partly be 0.22 micron millipore filtration membrane filtration removal liquid with the breeding of leaching bacteria solution left standstill with the aperture with supernatant liquor, the bacterium of acquisition is put into the 9K substratum, and to make bacterial concentration be 10
8The bacterium liquid of cell/mL, what obtain seed selection soaks the cobalt bacterium.
The weight that adds cattierite accounts in 10% the Bioleaching of breeding of leaching bacteria total solution weight, and the leaching yield of cobalt is 94.1%, and the leaching yield of not taming the bacterium cobalt is 60.7%, and the leaching yield of soaking cobalt bacterium cobalt of seed selection can improve 33.4%.
Embodiment 6
The sulfuration cobalt ore that adopts is cobaltite;
Adopting concentration when regulating the pH value is the sulphuric acid soln of 1N;
The preparation method is:
1, gather in the pit water on cobalt ore mountain and soak ore deposit bacterium bacterium liquid, described to soak the ore deposit bacterium be the mixed bacterium that contains the little spirillum of iron protoxide, has a liking for the ferrous thiobacillus of acid oxidase and Acidithiobacillus thiooxidans;
2, will soak ore deposit bacterium bacterium liquid at the 9K inoculation of medium, inoculation is invaded ore deposit bacterium bacterium liquid and is accounted for 20% of cumulative volume, regulate pH value to 2.5, in airbath constant temperature oscillation case, under temperature 60 C and rotating speed 190rpm condition, carry out shaking culture one time, when soaking the ore deposit bacteria growing to logarithmic phase, to soak ore deposit bacterium bacterium liquid takes out and puts into the 9K substratum again and carry out the secondary shaking culture by above-mentioned condition, when soaking the ore deposit bacteria growing to logarithmic phase, will soak ore deposit bacterium bacterium liquid and take out and put into again the 9K substratum and carry out shaking culture three times by above-mentioned condition;
3, be that 0.22 micron filtering with microporous membrane is removed liquid with the ore deposit bacterium bacterium liquid that soaks after three shaking culture with the aperture, bacterium after obtaining to cultivate, will cultivating rear bacterium with the 9K substratum, to be diluted to concentration be 10
8Cell/mL obtains inoculation bacterium liquid;
4, will inoculate bacterium liquid puts into the 9K substratum and makes inoculation solution, wherein inoculate bacterium liquid and account for 5% of cumulative volume, 9K cultivates 95% of fiduciary point cumulative volume, regulate the pH value to 2.5 of inoculation solution, adding rose vitriol to concentration of cobalt ions is 1g/L again, then in airbath constant temperature oscillation case, under 44 ℃ of temperature and rotating speed 240rpm condition, once tame, to rise to concentration be 10 to bacterial number in inoculation solution
8When cell/mL is above, and ferrous ion is whole when oxidized in the 9K substratum, and bacterium liquid is taken out by tame next time, adds rose vitriol to concentration of cobalt ions at every turn when taming and manys 1g/L than last domestication, carries out altogether obtaining to tame bacterium liquid after 35 domestications;
5, will tame and put into the 9K substratum after bacterium liquid takes out and make seed selection solution, wherein tame bacterium liquid and account for 10% of seed selection overall solution volume, 9K cultivates 90% of fiduciary point seed selection overall solution volume, regulate the pH value to 2.5 of seed selection solution, then in airbath constant temperature oscillation case, under 44 ℃ of temperature and rotating speed 240rpm condition, carry out seed selection;
6, whole when oxidized when ferrous ion in the seed selection solution, in seed selection solution, add cobaltite, cobaltite accounts for 1% of seed selection total solution weight; The quantity growth to 10 of bacterium in seed selection solution
8When cell/mL was above, the seed selection solution of taking-up 1/4 was as bacterium liquid for subsequent use;
7, bacterium liquid for subsequent use is put into iron-free 9K substratum and make breeding of leaching bacteria solution, iron-free 9K cultivates 90% of fiduciary point breeding of leaching bacteria overall solution volume, and all the other are bacterium liquid for subsequent use; Add cobaltite in breeding of leaching bacteria solution, the weight of cobaltite accounts for 2% of breeding of leaching bacteria total solution weight; Regulate the pH value to 2.5 of breeding of leaching bacteria solution, then in airbath constant temperature oscillation case, under 44 ℃ of temperature and rotating speed 240rpm condition, carry out breeding of leaching bacteria;
8, the quantity growth to 10 of bacterium in breeding of leaching bacteria solution
8When cell/mL is above, the breeding of leaching bacteria solution of taking-up 1/4 is as bacterium liquid for subsequent use, repeat to carry out breeding of leaching bacteria by the mode of step 7, the gross weight that each weight that adds cobaltite accounts for breeding of leaching bacteria solution Duos 1% than the last time, and the weight that adds for the last time cobaltite accounts for 10% of breeding of leaching bacteria total solution weight; After last breeding of leaching bacteria finishes, to solid precipitation, then partly be 0.22 micron millipore filtration membrane filtration removal liquid with the breeding of leaching bacteria solution left standstill with the aperture with supernatant liquor, the bacterium of acquisition is put into the 9K substratum, and to make bacterial concentration be 10
8The bacterium liquid of cell/mL, what obtain seed selection soaks the cobalt bacterium.
The weight that adds cobaltite accounts in 10% the Bioleaching of breeding of leaching bacteria total solution weight, and the leaching yield of cobalt is 91.7%, and the leaching yield of not taming the bacterium cobalt is 56.4%, and the leaching yield of soaking cobalt bacterium cobalt of seed selection can improve 35.3%.
Embodiment 7
The sulfuration cobalt ore that adopts is cattierite;
Adopting concentration when regulating the pH value is the sulphuric acid soln of 1N;
The preparation method is:
1, gather in the pit water on cobalt ore mountain and soak ore deposit bacterium bacterium liquid, described to soak the ore deposit bacterium be the mixed bacterium that contains the little spirillum of iron protoxide, has a liking for the ferrous thiobacillus of acid oxidase and Acidithiobacillus thiooxidans;
2, will soak ore deposit bacterium bacterium liquid at the 9K inoculation of medium, inoculation is invaded ore deposit bacterium bacterium liquid and is accounted for 20% of cumulative volume, regulate pH value to 2.0, in airbath constant temperature oscillation case, under temperature 60 C and rotating speed 190rpm condition, carry out shaking culture one time, when soaking the ore deposit bacteria growing to logarithmic phase, to soak ore deposit bacterium bacterium liquid takes out and puts into the 9K substratum again and carry out the secondary shaking culture by above-mentioned condition, when soaking the ore deposit bacteria growing to logarithmic phase, will soak ore deposit bacterium bacterium liquid and take out and put into again the 9K substratum and carry out shaking culture three times by above-mentioned condition;
3, be that 0.22 micron filtering with microporous membrane is removed liquid with the ore deposit bacterium bacterium liquid that soaks after three shaking culture with the aperture, bacterium after obtaining to cultivate, will cultivating rear bacterium with the 9K substratum, to be diluted to concentration be 10
8Cell/mL obtains inoculation bacterium liquid;
4, will inoculate bacterium liquid puts into the 9K substratum and makes inoculation solution, wherein inoculate bacterium liquid and account for 5% of cumulative volume, 9K cultivates 95% of fiduciary point cumulative volume, regulate the pH value to 2.0 of inoculation solution, adding rose vitriol to concentration of cobalt ions is 1g/L again, then in airbath constant temperature oscillation case, under 52 ℃ of temperature and rotating speed 200rpm condition, once tame, to rise to concentration be 10 to bacterial number in inoculation solution
8When cell/mL is above, and ferrous ion is whole when oxidized in the 9K substratum, and bacterium liquid is taken out by tame next time, adds rose vitriol to concentration of cobalt ions at every turn when taming and manys 1g/L than last domestication, carries out altogether obtaining to tame bacterium liquid after 35 domestications;
5, will tame and put into the 9K substratum after bacterium liquid takes out and make seed selection solution, wherein tame bacterium liquid and account for 7% of seed selection overall solution volume, 9K cultivates 93% of fiduciary point seed selection overall solution volume, regulate the pH value to 2.0 of seed selection solution, then in airbath constant temperature oscillation case, under 52 ℃ of temperature and rotating speed 200rpm condition, carry out seed selection;
6, whole when oxidized when ferrous ion in the seed selection solution, in seed selection solution, add cattierite, cattierite accounts for 1% of seed selection total solution weight; The quantity growth to 10 of bacterium in seed selection solution
8When cell/mL was above, the seed selection solution of taking-up 1/4 was as bacterium liquid for subsequent use;
7, bacterium liquid for subsequent use is put into iron-free 9K substratum and make breeding of leaching bacteria solution, iron-free 9K cultivates 93% of fiduciary point breeding of leaching bacteria overall solution volume, and all the other are bacterium liquid for subsequent use; Add cattierite in breeding of leaching bacteria solution, the weight of cattierite accounts for 2% of breeding of leaching bacteria total solution weight; Regulate the pH value to 1.2 of breeding of leaching bacteria solution, then in airbath constant temperature oscillation case, under 52 ℃ of temperature and rotating speed 200rpm condition, carry out breeding of leaching bacteria;
8, the quantity growth to 10 of bacterium in breeding of leaching bacteria solution
8When cell/mL is above, the breeding of leaching bacteria solution of taking-up 1/4 is as bacterium liquid for subsequent use, repeat to carry out breeding of leaching bacteria by the mode of step 7, the gross weight that each weight that adds cattierite accounts for breeding of leaching bacteria solution Duos 1% than the last time, and the weight that adds for the last time cattierite accounts for 10% of breeding of leaching bacteria total solution weight; After last breeding of leaching bacteria finishes, to solid precipitation, then partly be 0.22 micron millipore filtration membrane filtration removal liquid with the breeding of leaching bacteria solution left standstill with the aperture with supernatant liquor, the bacterium of acquisition is put into the 9K substratum, and to make bacterial concentration be 10
8The bacterium liquid of cell/mL, what obtain seed selection soaks the cobalt bacterium.
The weight that adds cattierite accounts in 10% the Bioleaching of breeding of leaching bacteria total solution weight, and the leaching yield of cobalt is 92.8%, and the leaching yield of not taming the bacterium cobalt is 60.7%, and the leaching yield of soaking cobalt bacterium cobalt of seed selection can improve 32.1%.
Claims (3)
1. selection that soaks the cobalt bacterium is characterized in that carrying out according to the following steps:
(1) gather in the pit water on cobalt ore mountain and soak ore deposit bacterium bacterium liquid, described to soak the ore deposit bacterium be the mixed bacterium that contains the little spirillum of iron protoxide, has a liking for the ferrous thiobacillus of acid oxidase and Acidithiobacillus thiooxidans;
(2) will soak ore deposit bacterium bacterium liquid at the 9K inoculation of medium, inoculation is soaked ore deposit bacterium bacterium liquid and is accounted for 20% of cumulative volume, regulate pH value to 1.2 ~ 2.5, in airbath constant temperature oscillation case, under temperature 60 C and rotating speed 190rpm condition, carry out shaking culture one time, when soaking the ore deposit bacteria growing to logarithmic phase, to have a liking for leaching-ore bacteria bacterium liquid takes out and to put into the 9K substratum again and carry out the secondary shaking culture by above-mentioned condition, when soaking the ore deposit bacteria growing to logarithmic phase, will soak ore deposit bacterium bacterium liquid and take out and put into again the 9K substratum and carry out shaking culture three times by above-mentioned condition;
(3) be that 0.22 micron filtering with microporous membrane is removed liquid with the ore deposit bacterium bacterium liquid that soaks after three shaking culture with the aperture, bacterium after obtaining to cultivate, will cultivating rear bacterium with the 9K substratum, to be diluted to concentration be 10
8Cell/mL obtains inoculation bacterium liquid;
(4) will inoculate bacterium liquid puts into the 9K substratum and makes inoculation solution, wherein inoculate bacterium liquid and account for 5% of cumulative volume, 9K cultivates 95% of fiduciary point cumulative volume, the pH value to 1.2 of adjusting inoculation solution ~ 2.5, adding rose vitriol to concentration of cobalt ions is 1g/L again, then in airbath constant temperature oscillation case, under 44 ~ 60 ℃ of temperature and rotating speed 150 ~ 240rpm condition, once tame, to rise to concentration be 10 to bacterial number in inoculation solution
8When cell/mL is above, and ferrous ion is whole when oxidized in the 9K substratum, bacterium liquid is taken out tame next time, adds rose vitriol to concentration of cobalt ions at every turn when taming and manys 1g/L than last domestication, carries out altogether obtaining to tame bacterium liquid after 35 domestications;
(5) will tame and put into the 9K substratum after bacterium liquid takes out and make seed selection solution, wherein tame bacterium liquid and account for 5 ~ 10% of seed selection overall solution volume, 9K cultivates 90 ~ 95% of fiduciary point seed selection overall solution volume, the pH value to 1.2 of adjusting seed selection solution ~ 2.5, then in airbath constant temperature oscillation case, under 44 ~ 60 ℃ of temperature and rotating speed 150 ~ 240rpm condition, carry out seed selection;
(6) whole when oxidized when ferrous ion in the seed selection solution, in seed selection solution, add the sulfuration cobalt ore, the weight of sulfuration cobalt ore accounts for 1% of seed selection total solution weight; The quantity growth to 10 of bacterium in seed selection solution
8When cell/mL was above, the seed selection solution of taking-up 1/4 was as bacterium liquid for subsequent use;
(7) bacterium liquid for subsequent use is put into iron-free 9K substratum and make breeding of leaching bacteria solution, iron-free 9K cultivates 90 ~ 95% of fiduciary point breeding of leaching bacteria overall solution volume, and all the other are bacterium liquid for subsequent use; Add the sulfuration cobalt ore in breeding of leaching bacteria solution, the weight of sulfuration cobalt ore accounts for 2% of breeding of leaching bacteria total solution weight; Breeding of leaching bacteria in airbath constant temperature oscillation case, is carried out in the pH value to 1.2 of adjusting breeding of leaching bacteria solution ~ 2.5 then under 44 ~ 60 ℃ of temperature and rotating speed 150 ~ 240rpm condition;
(8) quantity growth to 10 of bacterium in breeding of leaching bacteria solution
8When cell/mL is above, the breeding of leaching bacteria solution of taking-up 1/4 is as bacterium liquid for subsequent use, repeat to carry out breeding of leaching bacteria by the mode of step 7, the gross weight that each weight that adds the sulfuration cobalt ore accounts for breeding of leaching bacteria solution Duo 1% than last, and the weight that cobalt ore is vulcanized in last adding accounts for 10% of breeding of leaching bacteria total solution weight; After last breeding of leaching bacteria finishes, to solid precipitation, then partly be 0.22 micron millipore filtration membrane filtration removal liquid with the breeding of leaching bacteria solution left standstill with the aperture with supernatant liquor, the bacterium of acquisition is put into the 9K substratum, and to make bacterial concentration be 10
8The bacterium liquid of cell/mL, what obtain seed selection soaks the cobalt bacterium.
2. a kind of selection that soaks the cobalt bacterium according to claim 1, employing concentration is the sulphuric acid soln of 1N when it is characterized in that regulating pH value in the described method.
3. a kind of selection that soaks the cobalt bacterium according to claim 1 is characterized in that described sulfuration cobalt ore is corallite, linnaeite, cobaltite, cobalt pyrite or cattierite.
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| CN103572048A (en) * | 2013-11-19 | 2014-02-12 | 东北大学 | Method of cobalt leaching with activated carbon catalysis bacteria |
| CN103572049A (en) * | 2013-11-19 | 2014-02-12 | 东北大学 | Bacterium agitation leaching method of cobalt concentrate |
| CN104293727A (en) * | 2014-06-20 | 2015-01-21 | 江西省科学院生物资源研究所 | Method suitable for long-term domestication and preservation of arsenic resistant bioleaching florae at different temperatures |
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| CN1350058A (en) * | 2001-12-06 | 2002-05-22 | 中南大学 | Method of breeding strain for soaking ore |
| CN101792853A (en) * | 2010-02-10 | 2010-08-04 | 东北大学 | Bacterial oxidation pretreatment and cyanidation gold extraction method of arsenopyrite and realgar type refractory gold ores |
| CN101974461A (en) * | 2010-10-20 | 2011-02-16 | 中南大学 | Bioleaching strain breeding method for copper ore agitation leaching |
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| CN1350058A (en) * | 2001-12-06 | 2002-05-22 | 中南大学 | Method of breeding strain for soaking ore |
| CN101792853A (en) * | 2010-02-10 | 2010-08-04 | 东北大学 | Bacterial oxidation pretreatment and cyanidation gold extraction method of arsenopyrite and realgar type refractory gold ores |
| CN101974461A (en) * | 2010-10-20 | 2011-02-16 | 中南大学 | Bioleaching strain breeding method for copper ore agitation leaching |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103572048A (en) * | 2013-11-19 | 2014-02-12 | 东北大学 | Method of cobalt leaching with activated carbon catalysis bacteria |
| CN103572049A (en) * | 2013-11-19 | 2014-02-12 | 东北大学 | Bacterium agitation leaching method of cobalt concentrate |
| CN103572049B (en) * | 2013-11-19 | 2015-07-01 | 东北大学 | Bacterium agitation leaching method of cobalt concentrate |
| CN103572048B (en) * | 2013-11-19 | 2015-07-01 | 东北大学 | Method of cobalt leaching with activated carbon catalysis bacteria |
| CN104293727A (en) * | 2014-06-20 | 2015-01-21 | 江西省科学院生物资源研究所 | Method suitable for long-term domestication and preservation of arsenic resistant bioleaching florae at different temperatures |
| CN104293727B (en) * | 2014-06-20 | 2018-01-05 | 江西省科学院生物资源研究所 | The method for taming and preserving for a long time anti-arsenic mineral-leaching bacteria colony suitable for various temperature |
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