CN102002588A - Bioleaching method-uranium leaching from fungus - Google Patents
Bioleaching method-uranium leaching from fungus Download PDFInfo
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Abstract
The invention relates to a bioleaching method-uranium leaching from fungus. The method comprises the following steps of: using chemical reagents or fermenting low-price carbon source straws to generate fungus of organic acids of citric acid, oxalic acid, malic acid and lactic acid; and leaching uranium out from uranium tailings, tailing residues, uranium barren rocks or uranium ores by utilizing the fungus (1),or fermentation liquor (2) of the fungus, or a fungus mixed fermentation liquor (3), or by adding chemical reagents, such as sulfuric acid and hydrochloric acid to the fungus (1), or the fermentation liquor (2) of the fungus, or the fungus-mixed fermentation liquor (3). The invention has the advantages of convenient material obtainment, low cost, simple and convenient processing steps environmentally-friendly performance, energy saving and the like.
Description
Technical field
The present invention relates to a kind of bioleaching method of environmental protection, is to utilize to produce the method that organic acid fungies such as citric acid, oxalic acid, oxysuccinic acid, lactic acid are leached the uranium in uranium tailings, mine tailings, uranium barren rock or the uranium ore.
Background technology
Uranium is the raw material of nuclear power and Nuclear weapons, and the national economic development and national defense construction are had vital role.Current, China's positive develop actively nuclear power cause increases day by day to the demand of natural uranium.Yet along with exploitation for many years, the high grade uranium ore resource is poor day by day, and present mining grade only is about 0.1%; The two big main process that natural uranium is produced, face of land dump leaching carry uranium and the original place leaching is adopted the leaching yield of uranium respectively less than 85% and 60%.Simultaneously, the acid system extract technology that adopts not only to installations and facilities requirement for anticorrosion height, and has produced a large amount of acid waste water at present, and environment has been caused serious threat.Therefore, development environment close friend's the new and effective uranium method of soaking is extremely urgent.
Microbial leaching is a kind of novel process of utilizing microbial process to combine with hydrometallurgy, is microbiology and the new technology that forms of mining technique subject crossing in modern age.It has that production cost is low, less investment, technical process is short, equipment is simple, eco-friendly characteristics, is particularly suitable for the low-grade mineral resource processing and utilization.Though it is a kind of effective means that microorganism is leached, there is following outstanding problem in present technology: bacterial growth is slow, and the metabolism time is long; Only be suitable for the leaching of sulphide ores, and China's uranium ore resource is based on low-grade non-sulfide ore; Can not reduce the acid waste water of generation.
A kind of new biology of this seminar invention soaks uranium method-fungi and soaks uranium and can solve above problem.
Discover that many fungies can produce lot of organic acids in culturing process, these organic acids can form stable complex compound with uranium, thereby reach the purpose that uranium is leached from ore.This seminar selects some volume production organic acid fungies greatly through constantly screening, uses these fungies again and soaks the uranium experiment, has selected and has soaked uranium effect fungi preferably, and making fungi soak uranium becomes possibility.Because fungi can be used the cheap material of price such as stalk and be carried out large scale fermentation and cultivate, thus can reduce cost greatly, high grade ore not only, low grade ore and uranium tailings, uranium barren rock etc. have also all had extraction value, have widened the scope of utilizing of uranium resources.And mycetogenetic is relatively mild organic acid, so can alleviate pollution greatly to environment.
Summary of the invention
At above situation, this patent is a research object with the uranium ore, has invented a kind of method of utilizing fungi that the uranium in the uranium ore is leached.This invention has multiple advantages such as material is convenient, with low cost, simple processing step, environmental friendliness, energy conservation.
This method utilizes chemical reagent or the cheap carbon source through fermentation of stalk to produce citric acid, oxalic acid, oxysuccinic acid, lactic acid organic acid fungi, use 1. fungi or 2. its fermented liquid or 3. fungi mixed fermentation liquid or, the uranium in uranium tailings, mine tailings, uranium barren rock or the uranium ore is leached 1. or 2. or add chemical reagent such as sulfuric acid, hydrochloric acid on the basis 3..Six kinds of modes that obtain the uranium leach liquor are arranged, are specially:
1) direct uranium ore is joined inoculated in the substratum that produces sour fungi, and uranium is also leached in fungi constantly enrichment in ore pulp, product acid, cultivates the end after-filtration and obtains the uranium leach liquor;
2) large scale fermentation is cultivated fungi, filters and removes mycelium, drips with filtrate and drenches the ore deposit heap, collects the uranium leach liquor;
3) the utilization liquid nutrient medium drips and drenches the ore deposit heap, collects the uranium leach liquor;
4) large scale fermentation is cultivated fungi, filters and removes mycelium, drips with filtrate and drenches the ore deposit heap, leaches and drips pouring with dilute sulphuric acid latter stage, collects the uranium leach liquor;
5) the utilization liquid nutrient medium drips and drenches the ore deposit heap, leaches to drip to drench to help with dilute sulphuric acid or other chemical reagent latter stage and soaks, and collects filtrate;
6) large scale fermentation is cultivated fungi, filters and removes mycelium, adds dilute sulphuric acid or other chemical reagent and drips pouring ore deposit heap, collects the uranium leach liquor.
The measure of collecting after the filtrate is:
The filtrate of collecting selects the resins exchange method uranium leach liquor to be carried out the recovery of uranium after detecting through uranium concentration, pH value, current potential.
The present invention is a raw material with cheap material such as fungi, stalk and uranium ore, has solved the problem that the exploitation difficulty is big, low in economic efficiency, environmental pollution is big that present uranium mining exists.
The fungi of this patent invention is soaked the uranium method, and method has following technical superiority compared to existing technology;
(1) fungi is soaked uranium provides a kind of feasible reoovery method to the uranium ore that can't develop with traditional method.
(2) fungi to soak uranium less to the pollution of environment.
(3) fungi is soaked the raw material sources convenience of uranium, and fungi is cultured continuously constantly, has saved cost and time.
(4) fungal growth speed is fast, and growth conditions is required simply, is easy to cultivate.
(5) the fungi adaptive faculty is strong, can be used for the leaching of various uranium ores.
(6) this method is not only applicable to the leaching of high grade uranium ore, and is applicable to the development and use of uranium-bearing matrix such as uranium tailings, mine tailings, uranium barren rock and low-grade uranium ore, has enlarged utilization of resources scope, has improved resource recovery.
Embodiment
The present invention is further illustrated below in conjunction with embodiment.
The I material is formed
Cheap carbon source, uranium ores such as fungi, chemical reagent or stalk.
The II principle
It mainly is that the uranium in the uranium ore is leached in a large amount of acid that using fungus produces in culturing process that fungi is soaked uranium, and this has also brought into play bigger effect fungi in leaching process.
Directly effect: fungi helps to destroy the lattice of ore attached on the ore, and can enlarge the ore crack, helps the carrying out of the complexing action of organic acid and uranium.
Indirect action: soak the uranium fungi and can produce organic acids such as citric acid, oxalic acid, oxysuccinic acid, lactic acid, these organic acids have coordinate bonds such as carboxyl, can complexing be arranged with uranium, thereby uranium is extracted from ore.
The III embodiment
A kind of method of utilizing fungi to leach uranium ore.Its concrete steps are:
(1) fungi in the uranium ore water is carried out enrichment culture, filter out the strong fungi of acid producing ability, it is carried out enrichment and protects kind.
(2) fungi that filters out is soaked uranium research, filter out and soak the effective bacterial strain of uranium.
(3) with low-price carbon source large scale fermentation fungies such as stalks.
(4) get culture or filter after nutrient solution soak the ore deposit, collect the uranium leach liquor.
(5) measure filtrate volume, uranium ion concentration, pH value and Eh value.
(6) uranium of the qualified filtrate of recovery.
IV embodiment
Embodiment 1, get 745 ore deposits, 501 work area 5g and be milled to-200 purpose breezes and put in the 150mL Erlenmeyer flask, kraft paper seals, 121 ℃ of autoclaving 30min, add the PDA substratum and the 0.5mL aspergillus niger mother liquor of 100mL sterilization, put 30 ℃ of constant temperature air bath shaking table shaking culture.Cultivate after 7 days and take out, filter, get the slag specimen drying, be milled to-200 orders, analyze uranium content, calculate leaching yield.The result shows that leaching yield reaches 73.81%.
Embodiment 2, get 745 ore deposits, 501 work area 5g and be milled to-200 purpose breezes and put in the 150mL Erlenmeyer flask, kraft paper seals, 121 ℃ of autoclaving 30min, add the PDA substratum and the 0.5mL mould mother liquor of 100mL sterilization, put 30 ℃ of constant temperature air bath shaking table shaking culture.Cultivate after 7 days and take out, filter, get the slag specimen drying, be milled to-200 orders, analyze uranium content, calculate leaching yield.The result shows that leaching yield reaches 73.42%.
Embodiment 3, get 745 ore deposits, 501 work area 5g and be milled to-200 purpose breezes and put in the 150mL Erlenmeyer flask, kraft paper seals, 121 ℃ of autoclaving 30min, add the PDA substratum and the red yeast of 0.5mL of 100mL sterilization, put 30 ℃ of constant temperature air bath shaking table shaking culture.Cultivate after 7 days and take out, filter, get the slag specimen drying, be milled to-200 orders, analyze uranium content, calculate leaching yield.The result shows that leaching yield reaches 69.84%.
Embodiment 4, get 718 ore deposit 5g and are milled to-200 purpose breezes and put in the 150mL Erlenmeyer flask, and kraft paper seals, and 121 ℃ of autoclaving 30min add the PDA substratum and the 0.5mL aspergillus niger mother liquor of 100mL sterilization, put 30 ℃ of constant temperature air bath shaking table shaking culture.Cultivate after 7 days and take out, filter, get the slag specimen drying, be milled to-200 orders, analyze uranium content, calculate leaching yield.The result shows that leaching yield reaches 72.02%.
Embodiment 5, get 718 ore deposit 5g and are milled to-200 purpose breezes and put in the 150mL Erlenmeyer flask, and kraft paper seals, and 121 ℃ of autoclaving 30min add the PDA substratum and the 0.5mL mould mother liquor of 100mL sterilization, put 30 ℃ of constant temperature air bath shaking table shaking culture.Cultivate after 7 days and take out, filter, get the slag specimen drying, be milled to-200 orders, analyze uranium content, calculate leaching yield.The result shows that leaching yield reaches 79.57%.
Embodiment 6, get 718 ore deposit 5g and are milled to-200 purpose breezes and put in the 150mL Erlenmeyer flask, and kraft paper seals, and 121 ℃ of autoclaving 30min add the PDA substratum and the red yeast mother liquor of 0.5mL of 100mL sterilization, put 30 ℃ of constant temperature air bath shaking table shaking culture.Cultivate after 7 days and take out, filter, get the slag specimen drying, be milled to-200 orders, analyze uranium content, calculate leaching yield.The result shows that leaching yield reaches 58.6%.
Below only be better embodiment of the present invention, according to above-mentioned design of the present invention, those skilled in the art can also make various modifications and conversion to this.For example, adopt different fungies to soak uranium, utilize fungi that the uranium in uranium tailings, mine tailings, uranium barren rock or the uranium ore is exploited utilization, utilized fungi to carry out the exploitation of other metals, substratum to fungi is improved, adopt different culture condition and incubation time, the absorption property of utilization fungi handles and reclaims uranium etc. to uranium-containing waste water.Similar this conversion and modification all belong to essence of the present invention.
Claims (4)
1. a bioleaching method---fungi is soaked uranium, it is characterized in that: utilize the cheap carbon source through fermentation of chemical reagent or stalk to produce citric acid, oxalic acid, oxysuccinic acid, lactic acid organic acid fungi, use 1. fungi or 2. its fermented liquid or 3. fungi mixed fermentation liquid or, the uranium in uranium tailings, mine tailings, uranium barren rock or the uranium ore is leached 1. or 2. or add chemical reagent such as sulfuric acid, hydrochloric acid on the basis 3..
2. a kind of bioleaching method according to claim 1---fungi is soaked uranium, it is characterized in that, the concrete steps that fungi is soaked uranium are:
(1) fungi in the uranium ore water is carried out enrichment culture, filter out the strong fungi of acid producing ability, it is carried out enrichment and protects kind;
(2) fungi that filters out is soaked uranium research, filter out and soak the effective bacterial strain of uranium;
(3) with low-price carbon source large scale fermentation fungies such as chemical reagent or stalks;
(4) get culture or filter after nutrient solution or above-mentioned substance soak the ore deposit jointly, soak the ore deposit and can add chemical reagent such as sulfuric acid or hydrochloric acid and help and soak, collect leach liquor;
(5) measure leach liquor volume, uranium ion concentration, pH value and Eh value;
(6) uranium of the qualified filtrate of recovery.
3. a kind of bioleaching method according to claim 1---fungi is soaked uranium, it is characterized in that, obtains the mode of uranium leach liquor, and following mode is specifically arranged:
1) direct uranium ore is joined inoculated in the substratum that produces sour fungi, and uranium is also leached in fungi constantly enrichment in ore pulp, product acid, cultivates the end after-filtration and obtains the uranium leach liquor;
2) large scale fermentation is cultivated fungi, filters and removes mycelium, drips with filtrate and drenches the ore deposit heap, collects the uranium leach liquor;
3) the utilization liquid nutrient medium drips and drenches the ore deposit heap, collects the uranium leach liquor;
4) large scale fermentation is cultivated fungi, filters and removes mycelium, drips with filtrate and drenches the ore deposit heap, leaches and drips pouring with dilute sulphuric acid latter stage, collects the uranium leach liquor;
5) the utilization liquid nutrient medium drips and drenches the ore deposit heap, leaches to drip to drench to help with dilute sulphuric acid or other chemical reagent latter stage and soaks, and collects filtrate;
6) large scale fermentation is cultivated fungi, filters and removes mycelium, adds dilute sulphuric acid or other chemical reagent and drips pouring ore deposit heap, collects the uranium leach liquor.
4. a kind of bioleaching method according to claim 1---fungi is soaked uranium, it is characterized in that, the uranium leach liquor is carried out the recovery of uranium with the method for resins exchange.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012139166A1 (en) * | 2011-04-13 | 2012-10-18 | Bioheap Limited | Leach process |
| CN104060113A (en) * | 2014-06-26 | 2014-09-24 | 东华理工大学 | Novel pool-type process for removing alkali of alkaline uranium ore by using hydrochloric acid |
| CN104533346A (en) * | 2014-12-22 | 2015-04-22 | 核工业北京化工冶金研究院 | Method for preparing hole flushing agent through citric acid in in-situ leaching uranium mining process |
| CN104711439A (en) * | 2014-12-22 | 2015-06-17 | 核工业北京化工冶金研究院 | In-situ leaching uranium mining method by using mixing organic acidity as leaching agent |
| CN104046807B (en) * | 2014-06-26 | 2015-12-02 | 东华理工大学 | A kind of pool animalcule leaching uranium method |
| CN108285981A (en) * | 2018-02-09 | 2018-07-17 | 南华大学 | A kind of burned-coal fly ash puies forward uranium method |
| CN113337409A (en) * | 2021-07-26 | 2021-09-03 | 东华理工大学 | Aspergillus niger strain 738Y-15MJ and application thereof |
| CN114233258A (en) * | 2021-12-08 | 2022-03-25 | 核工业二三O研究所 | Fracturing method for difficult-to-leach sandstone uranium deposit reservoir transformation |
| CN117265300A (en) * | 2023-11-23 | 2023-12-22 | 核工业北京化工冶金研究院 | Method for improving reaction rate of neutral in-situ leaching uranium mining |
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2012243436B2 (en) * | 2011-04-13 | 2015-01-29 | Bioheap Limited | Leach process |
| WO2012139166A1 (en) * | 2011-04-13 | 2012-10-18 | Bioheap Limited | Leach process |
| AU2012243436C1 (en) * | 2011-04-13 | 2022-02-03 | Bioheap Limited | Leach process |
| CN104046807B (en) * | 2014-06-26 | 2015-12-02 | 东华理工大学 | A kind of pool animalcule leaching uranium method |
| CN104060113A (en) * | 2014-06-26 | 2014-09-24 | 东华理工大学 | Novel pool-type process for removing alkali of alkaline uranium ore by using hydrochloric acid |
| CN104060113B (en) * | 2014-06-26 | 2015-09-02 | 东华理工大学 | A kind of Alkaline uranium ore stone pool hydrochloric acid lixiviating technique |
| CN104533346A (en) * | 2014-12-22 | 2015-04-22 | 核工业北京化工冶金研究院 | Method for preparing hole flushing agent through citric acid in in-situ leaching uranium mining process |
| CN104711439A (en) * | 2014-12-22 | 2015-06-17 | 核工业北京化工冶金研究院 | In-situ leaching uranium mining method by using mixing organic acidity as leaching agent |
| CN108285981A (en) * | 2018-02-09 | 2018-07-17 | 南华大学 | A kind of burned-coal fly ash puies forward uranium method |
| CN108285981B (en) * | 2018-02-09 | 2020-05-19 | 南华大学 | Method for extracting uranium from fly ash of fire coal |
| CN113337409A (en) * | 2021-07-26 | 2021-09-03 | 东华理工大学 | Aspergillus niger strain 738Y-15MJ and application thereof |
| CN114233258A (en) * | 2021-12-08 | 2022-03-25 | 核工业二三O研究所 | Fracturing method for difficult-to-leach sandstone uranium deposit reservoir transformation |
| CN117265300A (en) * | 2023-11-23 | 2023-12-22 | 核工业北京化工冶金研究院 | Method for improving reaction rate of neutral in-situ leaching uranium mining |
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