CN106148244B - It is a kind of degrade waste lubricating oil Acidovorax avenae and purposes - Google Patents
It is a kind of degrade waste lubricating oil Acidovorax avenae and purposes Download PDFInfo
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Abstract
The present invention provides Acidovorax avenae and the purposes of a kind of waste lubricating oil that can effectively degrade.The Acidovorax avenae was preserved in Guangdong Province's Culture Collection on August 9th, 2016, and deposit number is GDMCC No:60061.The bacterial strain all has significant degradation effect to the waste lubricating oil of high concentration, used oil, useless transformer oil, waste hydraulic oil, the maximum concentration of degradable waste lubricating oil is 1304mg/L, degradable waste hydraulic oil, used oil, useless transformer oil maximum concentration be respectively 1204mg/L, 1105mg/L, 812mg/L.The bacterial strain applies also for carrying out biological oil removing processing to the oil-containing acid sludge generated in waste lubricant oil regeneration treatment process；Initial oil content is the acid sludge of 50-80mg/g or so, is sowed after the fermentation liquid of the Acidovorax avenae at 35-37 DEG C or so after processing in 2.5-3.5 months, oil content can be down to 1mg/g or less.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of Acidovorax avenae for the waste lubricating oil that can effectively degrade
And purposes.
Background technique
As process of industrialization is further speeded up, the demand and waste lubricating oil yield of lubricating oil are continuously increased.In view of
Global energy anxiety and stringent environmental requirement, waste lubricating oil must obtain regeneration, expedite the emergence of at waste lubricant oil regeneration
Set flourishing for industry.The regeneration disposition of waste lubricating oil experienced sulfuric acid-carclazyte, atmospheric and vacuum distillation, solvent refining, molecule steaming
The development of technical matters such as evaporate, these techniques are inevitably generated industrial residue.Waste residue contains the acid such as the complete sulfuric acid of unreacted
The harmful substances such as property substance, the object that coincides, sulfonated bodies, sulfide and nitride, forbid to discharge in every country.This part waste residue
So far effective processing means be there is no, no matter be not ideal method of disposal using burning or landfill disposal.
The method that processing waste residue mainly uses both at home and abroad at present all concentrates on physico-chemical process, and to using at bioanalysis
The report for managing acid sludge is less.The existing physico-chemical process for administering waste residue mainly has Low Temperature Thermal solution, fuel process, production fuel
Oil process, production white carbon black method, production petroleum antirust agent method, prepares anhydrous sodium sulfate method at hydrolysis-neutralisation.Patent application
CN1073965A discloses " a kind of method of acid sludge comprehensive utilization ", it is characterized in that making acid sludge be separated into oil, residue by heating
Three levels of slag and sour water have separated oil and the remaining slag of sour water after alkali neutralization, have added other additives to concoct together and be made
Interleaving agent, waterproofing unction and dry powder sulfonated gilsonite.Patent application CN1195564A discloses a kind of " side of acid sludge comprehensive utilization
Method ", it is characterised in that neutralizer is added in acid sludge, carries out process for producing and goes out fuel heavy oil, industrial saltcake, make in fuel heavy oil
Sulphur separate, solve problem of environmental pollution.Yang Kunyuan etc. discloses " hydrolysis-neutralisation processing lubrication oleic acid
The research of slag " (petroleum refining and chemical industry, 1998.6.18), in 2L there-necked flask and 500L reaction kettle, experimental study goes out lubricating oil
New Process for Treatment --- the hydrolysis-neutralisation that acid sludge is generated when waste oil regeneration, can be effectively by acid sludge point under demulsifier effect
From for residual oil and sour water, residual oil can reconcile with 20% catalytic cracking diesel oil and produce qualified No. 100 boiler oils or oxidation
Produce No. 30 building asphalts, sour water is neutralized, can be recycled after sedimentation, does not discharge to outside system, generation environment does not pollute, warp
It helps profitable.
Traditional physical chemistry method has the shortcomings that treatment conditions are harsh, treatment effect is bad, and bioanalysis has expense low
Honest and clean, high treating effect, it is without secondary pollution, can treatment in situ the advantages that.Using Biochemical method acid sludge main matter basis just
It is the microorganism with stronger degradation capability, and filters out in the microorganism more from type, quantity is big with stronger degradation
The strain of specific pollutants mass-energy power just seems especially difficult.
Summary of the invention
The object of the present invention is to provide it is a kind of with it is excellent degradation oil removing performance microorganism fungus kind and application thereof, to for
The biological treatment that acid sludge is generated in waste lubricant oil regeneration treatment process provides material base.
The technical solution that the present invention takes to achieve the goals above is as follows:
A kind of to screen the Acidovorax avenae isolated from waste lubricating oil, which has the recycling oil product such as lubricating oil
Higher biodegradability.The strain has been preserved in Guangdong Province's Culture Collection (referred to as on August 9th, 2016
GDMCC, address: No. 59 building of compound of Xianlie Middle Road, Guangzhou City 100, Guangdong institute of microbiology, postcode: 510075), preservation is compiled
Number be GDMCC No:60061, systematic name: Acidovorax avenae (Acidovorax avenae).
Acidovorax avenae's GDMCC No:60061 strain characteristic is as follows: bacillus, and bacterium colony is in yellow, opaque, colony edge
Neatly, median rise；Belong to medium temperature aerobic microbiological, can be grown by sole carbon source of lubricating oil；It is more demanding to dissolved oxygen, when
When shaking speed is lower than 150rpm, the growth metabolism in lubricating oil is obviously inhibited；PH is kept within the scope of 4.5-7.0
Higher vigor, the most suitable growth range is in 6.2-6.5 or so, and vigor sharply declines in alkaline environment；Environment temperature is at 20-42 DEG C
Within fluctuate, bacterial strain keeps good growth characteristics, but more than 42 DEG C, is difficult to give birth in the environment using lubricating oil as sole carbon source
It is long.
Acidovorax avenae of the present invention can be applied to degradation waste lubricating oil；The maximum degradable waste lubricating oil concentration of the bacterial strain
It is 35-37 DEG C of degradation temperature most suitable for 1304mg/L or so.
Acidovorax avenae of the present invention applies also for degradation waste hydraulic oil, used oil or useless transformer oil；The strain
Degradable waste hydraulic oil, used oil, useless transformer oil maximum concentration respectively may be about the left side 1204mg/L, 1105mg/L, 812mg/L
It is right.
Acidovorax avenae of the present invention applies also for the oil-containing acid sludge generated in waste lubricant oil regeneration treatment process
Carry out biological oil removing processing；Initial oil content is the acid sludge of 50-80mg/L or so, after the fermentation liquid for sowing the Acidovorax avenae
At 35-37 DEG C or so after processing in 2.5-3.5 months, oil content can be down to 1mg/g or less.
The utility model has the advantages that
The present invention provides a kind of Acidovorax avenaes with excellent degradation oil removing performance, can be using lubricating oil as sole carbon
Source growth, and the waste lubricating oil to high concentration, used oil, useless transformer oil, waste hydraulic oil all have significant degradation effect.This
Invention further utilizes the oil removing characteristic of the bacterial strain, provides the acid sludge that waste lubricant oil regeneration treatment process generates a kind of feasible
Bioremediation, treated, and acid sludge oil content is effectively controlled, generally in 1-2.5mg/g hereinafter, and avoid with
The many drawbacks and unworthiness of past physical and chemical processing, this method have low-cost, high treating effect, without secondary pollution, behaviour
Make the advantages that easy.Environmental pollution is effectively prevented when carrying out waste utilization to treated acid sludge, and has saved and has been processed into
This, has made favorable attributes to the orderly development of waste lubricant oil regeneration treatment industry.
Detailed description of the invention
Fig. 1: most degradation capability study of the Acidovorax avenae to waste lubricating oil；
Fig. 2: oil content situation of change when Acidovorax avenae's degradation waste hydraulic oil；
Fig. 3: cell growth status when Acidovorax avenae's degradation waste hydraulic oil；
Fig. 4: oil content situation of change when Acidovorax avenae's degradation used oil；
Fig. 5: cell growth status when Acidovorax avenae's degradation used oil；
Fig. 6: Acidovorax avenae degrade useless transformer oil when oil content situation of change；
Fig. 7: Acidovorax avenae degrade useless transformer oil when cell growth status；
Fig. 8: biological oil removing situation of the acid sludge in 35 DEG C of isoperibols；
Fig. 9: biological oil removing situation of the acid sludge in room temperature environment.
Specific embodiment
Below by specific embodiment, it is further discussed below the present invention.Unless stated otherwise, it is not described in embodiment
Technological means can be realized with mode known in those skilled in the art.In addition, embodiment be interpreted as it is illustrative
, the range being not intended to limit the present invention, the spirit and scope of the invention are limited only by the claims that follow.For art technology
For personnel, under the premise of without departing substantially from spirit and scope of the present invention, to material component, dosage, the ruler in these embodiments
Very little, shape progress various modifications, replacement, improvement also belong to protection scope of the present invention, and specific defined by the present invention
Parameter should have admissible error range.
Unless stated otherwise, acid sludge and waste oil used are taken from the production of Chongqing City's senior lubricant in following embodiments
Sales company, the initial oil content mass concentration of acid sludge are 68.4mg/g or so, initial pH value 2.60, initial water content 35.8%.
Unless stated otherwise, used oat acidophil is strain GDMCC No:60061 in following embodiments.
Unless stated otherwise, the measuring method of acid sludge oil content is as follows.
(1) chloroform extract:
Acid sludge 10g is accurately weighed, is put it into band slug taper bottle.20mL chloroform is added into conical flask, and is added
Several minutes of lid shaking, stands 12~16 hours.After standing, conical flask is set hot dipping 1 hour in 50~55 DEG C of water-baths, hot dipping
Conical flask filtering is removed, filtrate is connected in the 100mL beaker of known weight.Same method hot dipping twice, about 10mL every time, 0.5
Hour, filtrate is connected in same beaker.It places the beaker in draught cupboard in 55~58 DEG C of water-baths, ventilation is concentrated into dry doubling and wipes
Outer wall steam, is placed in 60~75 DEG C of baking ovens 4 hours, and taking-up is placed on drier and is cooled to room temperature, and weighs, increased weight M1
For chloroform extract.
(2) saponification removal vegetable oil:
50mL 0.5molL is added into chloroform extract-1Potassium hydroxide-ethanol liquid is placed in soap in 65~75 DEG C of water-baths
Change hydrolysis 1 hour and stirs.Saponification finishes and removes beaker, and saponification liquor is connected to 250mL separatory funnel, add 50mL deionized water and
The beaker is rinsed before and after 50mL petroleum ether, washing lotion is connected to same separatory funnel, shakes several minutes of rear stratification, and lower layer's water phase is used
25mL petroleum ether extraction 1 time, merges petroleum ether extraction liquid twice and wash 2~3 times, each 50mL simultaneously shakes 1min.
(3) gravimetric detemination unsaponifiable matter total amount:
Petroleum ether extract is placed in the tool slug taper bottle for filling 15g anhydrous sodium sulfate, number is placed in shake of jumping a queue
Filtrate is connected to the beaker of known weight after minute, be placed in ventilation in 40~42 DEG C of water-baths of draught cupboard be concentrated into dry doubling wipe it is outer
Wall aqueous vapor, is placed in 60~70 DEG C of baking ovens 4 hours, takes out after drier is cooled to room temperature and weighs, increased weight M2It is as non-soap
Compound can represent the content of pollution acid sludge Central Plains oily substance.
(4) calculating of acid sludge biological oil removing rate
In formula:
Acid sludge initial oil content (mg/g) is that oil-containing accounts for the ratio between acid sludge, that is, the testing result before testing；Acid sludge remains oil-containing
Measure the testing result of (mg/g) for practical remaining oil content in acid sludge after biological degradation experiment, that is, after testing.
Embodiment 1
Most degradation capability study of the strain to waste lubricating oil.
It is research Acidovorax avenae for the most degradation ability of waste lubricating oil, by cell fermentation liquid with 8% inoculum concentration
Be inoculated in 50mL lubricating oil containing the various concentration waste oil of recycling (reclaimed oil treatment plant) minimal medium (lubricating oil it is initial
Concentration is respectively 1102,1208,1304,1402mg/L) in, initial pH value is adjusted to 6.20, and temperature is 35 DEG C, in 180r/min
Lower shaken cultivation carries out the shake flat experiment of Acidovorax avenae's degradation lubricating oil, monitors 2 indexs: the 1) lubricating oil in sample liquid respectively
Concentration simultaneously calculates lubricating oil degradation rate, and the height of lubricating oil degradation rate represents the power of strain degradation capability at this concentration；2)
Measure the OD of sample liquid600nmValue, OD600nmThe size of value reflects the cell growth status of strain at this concentration.Fig. 1 is reflected
The case where Acidovorax avenae's degradation different initial concentration lubricating oil, it is seen that when initial concentration is respectively 1102,1208,1304mg/L
It is degradable that realization in 216,288,312 hours is managed in time-division other places.Acidovorax avenae can degradable waste lubricating oil maximum concentration
Up to 1304mg/L or so.
To other concentration lubricating oil degradation situations under similarity condition: initial concentration of lubricating oil and degradable needing the time such as
Under, 800mg/L, 168h；900mg/L, 180h；1000mg/L, 192h；500mg/L, 96h；600mg/L, 144h；700mg/L,
156h；50mg/L, 36h；100mg/l, 48h；200mg/L, 60h.
Embodiment 2
Strain degradation waste hydraulic oil capability study.
It is research Acidovorax avenae for the degradation capability of waste hydraulic oil, cell fermentation liquid is inoculated with 8% inoculum concentration
Contain various concentration waste liquid depressor oil in 50mL minimal medium (actual concentrations of waste hydraulic oil be respectively 106mg/L,
204mg/L, 412mg/L, 608mg/L, 814mg/L, 1016mg/L, 1204mg/L, 1308mg/L) in, initial pH value is adjusted to
6.20, temperature is 35 DEG C, and shaken cultivation carries out the shake flat experiment of Acidovorax avenae's degradation waste hydraulic oil under 180r/min, respectively
Monitor 2 indexs: the 1) concentration of lubricating oil in sample liquid, 2) OD of sample liquid600nmValue.Fig. 2-3 reflects Acidovorax avenae's degradation not
The case where with initial concentration waste hydraulic oil, corresponds to the time of degradable needs and reaction solution cell under above-mentioned different initial concentrations
Concentration OD600nmValue difference is successively are as follows: 48h, 0.286；59h, 0.312；83h, 0.374；109h, 0.480；139h, 0.562；
173h, 0.668；209h, 0.846；The above degradation effect of oil content 1300mg/L is unobvious.Acidovorax avenae degradable can give up
The maximum concentration of hydraulic oil is up to 1204mg/L or so.
Embodiment 3
Strain degradation used oil capability study.
It is research Acidovorax avenae for the degradation capability of used oil, cell fermentation liquid is inoculated in 8% inoculum concentration
Minimal medium that 50mL contains various concentration used oil (actual concentrations of used oil be respectively 794mg/L, 989mg/L,
1105mg/L, 1193mg/L) in, initial pH value is adjusted to 6.20, and temperature is 35 DEG C, and shaken cultivation carries out swallow under 180r/min
Wheat acidophil is degraded the shake flat experiment of waste hydraulic oil, monitor 2 indexs respectively: 1) concentration of lubricating oil in sample liquid, 2) sample liquid
OD600nmValue.Fig. 4-5 reflects the case where Acidovorax avenae's degradation different initial concentration used oil, and Acidovorax avenae can drop completely
The maximum concentration of used oil is solved up to 1105mg/L or so, take around 209 hours it is degradable, and reaction solution cell is dense at this time
Spend OD600nmValue reaches maximum value 0.879.
Embodiment 4
Strain degradation gives up transformer oil capability study.
It is research Acidovorax avenae for the degradation capability for the transformer oil that gives up, cell fermentation liquid is connect with 8% inoculum concentration
(actual concentrations for the transformer oil that gives up are respectively 606mg/ to the minimal medium that kind contains the useless transformer oil of various concentration in 50mL
L, 812mg/L, 902mg/L) in, initial pH value is adjusted to 6.20, and temperature is 35 DEG C, and shaken cultivation carries out swallow under 180r/min
Wheat acidophil is degraded the shake flat experiment of waste hydraulic oil, monitor 2 indexs respectively: 1) concentration of lubricating oil in sample liquid, 2) sample liquid
OD600nmValue.Fig. 6-7 reflect Acidovorax avenae degrade different initial concentrations give up transformer oil the case where, Acidovorax avenae can be complete
The maximum concentration of degradable used oil up to 812mg/L or so, take around 210 hours it is degradable, and reaction solution is thin at this time
Born of the same parents' concentration OD600nmValue reaches maximum value 0.672.
Embodiment 5
Using Acidovorax avenae to the biological oil removing method of the acid sludge containing lubricating oil, include the following steps:
1) prepared by seed liquor: the Acidovorax avenae of slant preservation being seeded in LB culture medium after 2 activation, control the
Two generation fermentation liquid OD600nm=1.1-1.2, is used as inoculum.
2) acid sludge pre-processes: acid sludge tiling being opened, thickness is no more than 50cm, thereto uniform spray concentration 3% (m/v) urine
Plain solution and 0.2mol/L dipotassium hydrogen phosphate solution, wherein nitrogen and phosphorus element mass ratio is 10~15:1 or so, is stirred
It is even.
3) acid sludge oil removing: by second generation fermentation liquid with the dosage of 2-8% (v/m), uniformly sowing in acid sludge, and stirring is mixed
It is even；Irregular moisturizing makes acid sludge humid control in 40%-75% during biological treatment, and cannot have free water precipitation after moisturizing；
Acid sludge treatment can be carried out at 20-40 DEG C of environment temperature.
Further, acid sludge treatment temperature is 35-37 DEG C.
Further, respectively digging sooner or later daily during biological treatment, it is primary to stir.
Further, if acid sludge initial oil content can add 0.3% (v/v) Tween in pretreatment in 80mg/g or more
80, additive amount is 1-3mL/kg acid sludge, can promote acid sludge treatment effect.The initial oil content of acid sludge generally 80mg/g with
Under, the bad acid sludge oil content of individual twice pressing treatment effects is higher.
Further, according to the oil content of process cycle and acid sludge, above-mentioned 3% (m/ was generally added every 10-15 days or so
V) urea liquid and 0.2mol/L dipotassium hydrogen phosphate solution；Initial stage dosage is less, generally 100mL/10kg acid sludge；Close to mid-term
When, i.e., when oil content is down to initial half or so, dosage is increased to 200-300mL/10kg acid sludge；Later period oil content in processes
When being reduced to initial 30-40%, urea liquid uses the concentration of 5% (m/v), and it is left that dosage is increased to 500mL/10kg acid sludge
It is right.
Biological oil removing processing is carried out to acid sludge containing lubricating oil using the above method, is handled after starting weekly to two weeks, inspection
Survey an oil content.Initial oil content is the acid sludge of 50-80mg/g or so, and fermentation liquid sows dosage with 2%, at 35-37 DEG C
Left and right, oil content can control within 1mg/g after processing in 2.5-3.5 months, handle in room temperature environment, environment temperature
At 20 DEG C of -37 DEG C or so fluctuations (i.e. from spring, through summer, to autumn), the time through or so half a year, oil content be can control
Within 2.5mg/g；It is more than 80mg/L for individual initial oil contents, Deal with Time, which is appropriately extended, also can achieve above-mentioned effect
Fruit if can have certain promotion using a small amount of surfactant, effectively improves treatment effect among these, but dosage with
Minimal amount is advisable, unsuitable excessive.Moreover, when being such as lower than 15 DEG C, being also easy to produce plate if the pedotheque of processing is in low temperature environment
Phenomena such as knot, at this point, surfactant should not be used.
Embodiment 6
Acid sludge homoiotherm handles example.
Oat acidophilus of the about 4mL after re-activation is inoculated with into the plastic culture lattice marked as No. 1, No. 2 about 50g acid sludge
Fermented liquid, using urea as nitrogen source, using dipotassium hydrogen phosphate as phosphorus source, and the ratio of C:N:P is 100:15:1, and turning over frequency is 12
Hour is primary, is placed at 35 DEG C of constant temperature and is handled, and acid sludge water content maintains 30%, and monitoring Acidovorax avenae is not existing together
Manage the time when acid sludge oil content and calculate degradation rate.
Fig. 8 reflects biological oil removing situation of the waste lubricating oil oil refining acid sludge in 35 DEG C of isoperibols.Initial oil content is
The acid sludge of 68.4mg/g is after biological treatment in 63 days, oil content is down to 1mg/g hereinafter, biological oil removing significant effect.
Embodiment 7
Acid sludge room temperature environment temperature biological treatment example.
Oat of the about 4mL after re-activation is inoculated with into the plastic culture lattice marked as No. 1, No. 2, No. 3 about 50g acid sludge
Acidophilus fermented liquid, using urea as nitrogen source, using dipotassium hydrogen phosphate as phosphorus source, and the ratio of C:N:P is 100:15:1, turns over frequency
It is primary for 12 hours, it is placed under room temperature environment and is handled (environment temperature is in 20 DEG C of -37 DEG C or so fluctuations), acid sludge water content
It maintains 30%, monitor acid sludge oil content of the Acidovorax avenae in the different disposal time and calculates degradation rate.
Fig. 9 reflects biological oil removing situation of the acid sludge in room temperature environment, compared to the biological treatment under constant temperature (35 DEG C),
The then slow many of acid sludge biological oil removing processing at room temperature.After the biological treatment of 6 half a months, the oil-containing of acid sludge
Amount is down to 5mg/g or less by initial 68.4mg/g.Lubricating oil degradation trend fits like a glove with environment temperature in acid sludge, in temperature
Higher June to September, the mean temperature of environment are 35~38 DEG C or so, and biodegradation rate is quickly.
Claims (4)
1. a kind of Acidovorax avenae for waste lubricating oil of degrading, deposit number is GDMCC No:60061.
2. the purposes of Acidovorax avenae described in claim 1, which is characterized in that the Acidovorax avenae is for useless lubrication of degrading
Oil, waste hydraulic oil, used oil or useless transformer oil；Wherein, the Acidovorax avenae to waste lubricating oil, waste hydraulic oil, used oil,
Useless transformer oil can degradable maximum concentration be respectively 1304mg/L, 1204mg/L, 1105mg/L, 812mg/L, initial pH
6.2, most suitable degradation temperature is 35 DEG C.
3. the purposes of Acidovorax avenae described in claim 1, which is characterized in that the Acidovorax avenae is used for waste lubricating oil
The oil-containing acid sludge generated in regeneration process carries out biological oil removing processing.
4. the purposes of Acidovorax avenae as claimed in claim 3, which is characterized in that initial oil content is the acid of 50-80mg/L
Slag is sowed after the fermentation liquid of the Acidovorax avenae at 35-37 DEG C after processing in 2.5-3.5 months, and oil content can be down to
1mg/g or less.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101787355A (en) * | 2010-03-08 | 2010-07-28 | 北京师范大学 | Oat acidovorax avenae strain capable of biodegrading natural estrogen and application thereof |
| CN102173399A (en) * | 2011-03-08 | 2011-09-07 | 武汉工程大学 | Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile |
-
2016
- 2016-08-30 CN CN201610755552.2A patent/CN106148244B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101787355A (en) * | 2010-03-08 | 2010-07-28 | 北京师范大学 | Oat acidovorax avenae strain capable of biodegrading natural estrogen and application thereof |
| CN102173399A (en) * | 2011-03-08 | 2011-09-07 | 武汉工程大学 | Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile |
Non-Patent Citations (1)
| Title |
|---|
| 瓜类果斑嗜酸菌修复润滑油污染土壤实验研究;伍涛;《中国优秀硕士学位论文全文数据库 工程科技辑》;20160215(第2期);摘要和第5.1节 * |
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