CN111069237B - Medium-low grade phosphorite heap leaching method combining composite strain with waste biomass - Google Patents
Medium-low grade phosphorite heap leaching method combining composite strain with waste biomass Download PDFInfo
- Publication number
- CN111069237B CN111069237B CN201911328294.XA CN201911328294A CN111069237B CN 111069237 B CN111069237 B CN 111069237B CN 201911328294 A CN201911328294 A CN 201911328294A CN 111069237 B CN111069237 B CN 111069237B
- Authority
- CN
- China
- Prior art keywords
- liquid
- phosphorus
- bacteria
- cellulose
- culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 239000002367 phosphate rock Substances 0.000 title claims abstract description 78
- 238000002386 leaching Methods 0.000 title claims abstract description 61
- 239000002028 Biomass Substances 0.000 title claims abstract description 51
- 238000000034 method Methods 0.000 title claims abstract description 43
- 239000002131 composite material Substances 0.000 title claims abstract description 41
- 239000002699 waste material Substances 0.000 title claims abstract description 38
- OJMIONKXNSYLSR-UHFFFAOYSA-N phosphorous acid Chemical compound OP(O)O OJMIONKXNSYLSR-UHFFFAOYSA-N 0.000 title claims abstract description 37
- 239000007788 liquid Substances 0.000 claims abstract description 152
- 241000894006 Bacteria Species 0.000 claims abstract description 93
- 239000000843 powder Substances 0.000 claims abstract description 42
- 229920002678 cellulose Polymers 0.000 claims abstract description 36
- 239000001913 cellulose Substances 0.000 claims abstract description 36
- 230000001580 bacterial effect Effects 0.000 claims abstract description 35
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000011574 phosphorus Substances 0.000 claims abstract description 32
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 32
- 230000000593 degrading effect Effects 0.000 claims abstract description 28
- DLYUQMMRRRQYAE-UHFFFAOYSA-N tetraphosphorus decaoxide Chemical compound O1P(O2)(=O)OP3(=O)OP1(=O)OP2(=O)O3 DLYUQMMRRRQYAE-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000005507 spraying Methods 0.000 claims description 76
- 239000001963 growth medium Substances 0.000 claims description 45
- 229910019142 PO4 Inorganic materials 0.000 claims description 39
- 239000010902 straw Substances 0.000 claims description 38
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 33
- 239000010452 phosphate Substances 0.000 claims description 33
- 239000002245 particle Substances 0.000 claims description 29
- 238000002156 mixing Methods 0.000 claims description 26
- 239000008223 sterile water Substances 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 25
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 20
- 238000012216 screening Methods 0.000 claims description 19
- 239000002904 solvent Substances 0.000 claims description 19
- 239000002609 medium Substances 0.000 claims description 17
- 238000005273 aeration Methods 0.000 claims description 16
- 229910052564 epsomite Inorganic materials 0.000 claims description 16
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 14
- 229910052760 oxygen Inorganic materials 0.000 claims description 14
- 239000001301 oxygen Substances 0.000 claims description 14
- 230000003381 solubilizing effect Effects 0.000 claims description 14
- 230000009469 supplementation Effects 0.000 claims description 14
- 239000006228 supernatant Substances 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 239000007787 solid Substances 0.000 claims description 12
- 150000001875 compounds Chemical class 0.000 claims description 10
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 10
- 229920001817 Agar Polymers 0.000 claims description 9
- 239000008272 agar Substances 0.000 claims description 9
- YWEUIGNSBFLMFL-UHFFFAOYSA-N diphosphonate Chemical compound O=P(=O)OP(=O)=O YWEUIGNSBFLMFL-UHFFFAOYSA-N 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 9
- 239000002023 wood Substances 0.000 claims description 9
- 235000007164 Oryza sativa Nutrition 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 8
- 235000009566 rice Nutrition 0.000 claims description 8
- 238000002791 soaking Methods 0.000 claims description 8
- 239000002689 soil Substances 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 8
- 241000209140 Triticum Species 0.000 claims description 7
- 235000021307 Triticum Nutrition 0.000 claims description 7
- 230000015556 catabolic process Effects 0.000 claims description 7
- 238000006731 degradation reaction Methods 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 241000589516 Pseudomonas Species 0.000 claims description 6
- 241000219793 Trifolium Species 0.000 claims description 6
- 240000008042 Zea mays Species 0.000 claims description 6
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 6
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 6
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims description 6
- 229940041514 candida albicans extract Drugs 0.000 claims description 6
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 6
- 235000005822 corn Nutrition 0.000 claims description 6
- 229910052500 inorganic mineral Inorganic materials 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 239000011707 mineral Substances 0.000 claims description 6
- 238000000746 purification Methods 0.000 claims description 6
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims description 6
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims description 6
- 239000012138 yeast extract Substances 0.000 claims description 6
- 241001460073 Trichoderma asperellum Species 0.000 claims description 5
- 238000000926 separation method Methods 0.000 claims description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 3
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 108010010803 Gelatin Proteins 0.000 claims description 3
- 244000068988 Glycine max Species 0.000 claims description 3
- 235000010469 Glycine max Nutrition 0.000 claims description 3
- 239000007836 KH2PO4 Substances 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 claims description 3
- 239000008273 gelatin Substances 0.000 claims description 3
- 229920000159 gelatin Polymers 0.000 claims description 3
- 235000019322 gelatine Nutrition 0.000 claims description 3
- 235000011852 gelatine desserts Nutrition 0.000 claims description 3
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- CSNNHWWHGAXBCP-UHFFFAOYSA-L magnesium sulphate Substances [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 2
- 239000003513 alkali Substances 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 238000011161 development Methods 0.000 abstract description 6
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract description 5
- 229910052799 carbon Inorganic materials 0.000 abstract description 5
- 230000007613 environmental effect Effects 0.000 abstract description 5
- 238000012545 processing Methods 0.000 abstract description 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 21
- 244000005700 microbiome Species 0.000 description 13
- 239000011812 mixed powder Substances 0.000 description 12
- 239000010410 layer Substances 0.000 description 9
- 239000007864 aqueous solution Substances 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 241000209094 Oryza Species 0.000 description 7
- 238000012258 culturing Methods 0.000 description 7
- 239000002994 raw material Substances 0.000 description 7
- 239000007921 spray Substances 0.000 description 7
- 239000004698 Polyethylene Substances 0.000 description 6
- 230000003321 amplification Effects 0.000 description 6
- 238000009826 distribution Methods 0.000 description 6
- 230000007935 neutral effect Effects 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- -1 polyethylene Polymers 0.000 description 6
- 229920000573 polyethylene Polymers 0.000 description 6
- 238000005086 pumping Methods 0.000 description 6
- 238000005070 sampling Methods 0.000 description 5
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 229940088598 enzyme Drugs 0.000 description 4
- 239000008188 pellet Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000003860 storage Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000605222 Acidithiobacillus ferrooxidans Species 0.000 description 3
- 241000605272 Acidithiobacillus thiooxidans Species 0.000 description 3
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000011028 pyrite Substances 0.000 description 3
- NIFIFKQPDTWWGU-UHFFFAOYSA-N pyrite Chemical compound [Fe+2].[S-][S-] NIFIFKQPDTWWGU-UHFFFAOYSA-N 0.000 description 3
- 229910052683 pyrite Inorganic materials 0.000 description 3
- 239000002356 single layer Substances 0.000 description 3
- 238000005303 weighing Methods 0.000 description 3
- 235000017060 Arachis glabrata Nutrition 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- 235000010777 Arachis hypogaea Nutrition 0.000 description 2
- 235000018262 Arachis monticola Nutrition 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 241000605118 Thiobacillus Species 0.000 description 2
- 230000001651 autotrophic effect Effects 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000020232 peanut Nutrition 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 241000208140 Acer Species 0.000 description 1
- 244000046151 Acer negundo Species 0.000 description 1
- 235000004422 Acer negundo Nutrition 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- FGUUSXIOTUKUDN-IBGZPJMESA-N C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 Chemical compound C1(=CC=CC=C1)N1C2=C(NC([C@H](C1)NC=1OC(=NN=1)C1=CC=CC=C1)=O)C=CC=C2 FGUUSXIOTUKUDN-IBGZPJMESA-N 0.000 description 1
- 108010059892 Cellulase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-CUHNMECISA-N D-Cellobiose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-CUHNMECISA-N 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 241001226178 bacterium enrichment culture Species 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229940106157 cellulase Drugs 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000009920 chelation Effects 0.000 description 1
- 238000012822 chemical development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 150000007522 mineralic acids Chemical class 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B5/00—Operations not covered by a single other subclass or by a single other group in this subclass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/78—Recycling of wood or furniture waste
Landscapes
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Environmental & Geological Engineering (AREA)
- Botany (AREA)
- Mycology (AREA)
- Processing Of Solid Wastes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a method for heap leaching of medium and low grade phosphorite by combining a composite strain with waste biomass. According to the method, according to the characteristics of the medium-low-grade phosphorite and the waste biomass, the medium-low-grade phosphorite and the waste biomass are crushed into powder, mixed, heap-soaked and sprayed with the composite bacterial liquid, cellulose degrading bacteria in the composite bacterial liquid are used for decomposing the waste biomass, a carbon source capable of being directly utilized is provided for heterotrophic phosphorus-dissolving bacteria in the composite bacterial liquid, the medium-low-grade phosphorite is dissolved through the heterotrophic phosphorus-dissolving bacteria, and finally low-cost and large-batch development and utilization of the medium-low-grade phosphorite are achieved. The method has the advantages of strong processing capacity, environmental protection, low cost, simple process flow and the like, and the leaching rate of phosphorus (calculated by phosphorus pentoxide) in the middle-low grade phosphorite can reach over 58 percent after 9 months of heap leaching.
Description
Technical Field
The invention relates to the technical field of phosphorite resource development and utilization and waste biomass recycling, in particular to a method for heap leaching of medium and low grade phosphorite by combining a composite strain with waste biomass.
Background
China is one of the largest agricultural producing countries in the world, and produces more than 8 hundred million metric tons of residual straw every year. The waste biomass which exists in large quantity is a huge and utilizable energy source, wherein straw stalks, wheat stalks, corn stalks, peanut stalks, soybean stalks, rape stalks and the like are common and abundant agricultural residues, and the waste biomass is difficult to process and utilize and can only be incinerated, so that the pollution to the atmospheric environment is caused, the great waste of biomass resources is also caused, and the sustainable development of modern agriculture is hindered. Obviously, the full development and utilization of the waste biomass is beneficial to improving the air quality, reducing the environmental pollution and changing waste into valuables. At present, the development and utilization of waste biomass mainly focus on the preparation of bioethanol, methane and the like by fermentation.
The method has the advantages that the phosphorite resources in China are rich and the reserves are large, the reserves of the phosphorite are nearly 180 hundred million tons, however, the high-grade phosphorite resources in China are few, the middle and low-grade phosphorite is more, the easy-to-select phosphorite is less, the difficult-to-select phosphorite is more, the rich ore with the phosphorite grade of more than 30 percent in China is less than 10 percent of the total reserves, and the average grade of the phosphorite in China is about 17 percent. At present, the conventional mode of mineral separation of phosphorite in China is to utilize inorganic acid such as sulfuric acid and the like to carry out chemical treatment on the phosphorite so as to convert insoluble phosphorus in the phosphorite into soluble phosphorus. The chemical development method is high in cost, is only suitable for treating high-grade phosphorite, and is particularly important for developing and utilizing medium-grade and low-grade phosphorite along with gradual depletion of high-grade phosphorite resources. Although the utilization efficiency of the phosphorite can be improved by improving or improving the mineral separation process, the treatment is permanent but not permanent, and the exploitation cost of the phosphorite is also indirectly improved.
In order to meet the requirement of crops on phosphorus and improve the environmental problems that high-grade phosphorite is over developed and medium-low grade phosphorite is difficult to utilize and is increasingly serious, the microbial technology is utilized to treat the medium-low grade phosphorite and is gradually developed. The phosphorus dissolving bacteria are used for dissolving the medium-grade and low-grade phosphorus ores, and the phosphorus dissolving bacteria are considered to have higher efficiency and lower cost. From the current state of research, microorganisms with a phosphate solubilizing effect can be divided into two categories: inorganic chemoautotrophic microorganisms (autotrophic bacteria) and organic chemoheterotrophic microorganisms (heterotrophic bacteria). At present, few research reports about autotrophic lysophosphate bacteria are reported, mainly including Acidithiobacillus ferrooxidans (Acidithiobacillus ferrooxidans), Acidithiobacillus thiooxidans (Acidithiobacillus thiooxidans), and the like. Previous research reports show that under the condition of the existence of pyrite or sulfur powder, acidophilic thiobacillus ferrooxidans and acidophilic thiobacillus thiooxidans can generate sulfuric acid by oxidizing pyrite or sulfur powder, so that medium and low-grade phosphorite is further dissolved, and soluble phosphorus in the phosphorite is finally leached. WuBiao et al disclose a method for converting phosphorus in a phosphate ore mixed with pyrite into soluble phosphorus by using thiobacillus acidocaldarius (CN101434917A), and subsequently developed a strain of thiobacillus acidithiobacus resistant to phosphorus acidophilus and used for heap leaching of middle and low grade phosphate ore (CN 102102085B).
A great deal of research has been carried out at home and abroad on heterotrophic phosphate solubilizing bacteria, for example, pseudomonas, bacillus, aspergillus, penicillium and the like are known heterotrophic phosphate solubilizing bacteria which can solubilize phosphate ores through acidification or chelation, but carbon sources are required for growth, reproduction and physiological and biochemical metabolism of the strains. The carbon source commonly used in laboratories is glucose, which can provide energy for the growth and propagation of microorganisms, but is relatively expensive. Considering that the waste biomass contains components such as lignin, cellulose and hemicellulose, the waste biomass can be used as an alternative source of cheap carbon sources. So far, the biomass generally needs to be pretreated firstly when being used, and the pretreated biomass still cannot be directly used by phosphorus dissolving bacteria. If cellulose degrading bacteria are introduced, the produced cellulase is utilized to hydrolyze lignocellulose into cellobiose, glucose and other reducing sugars, so that the phosphorus dissolving bacteria utilize the saccharides as energy substances for growth to breed, and the purpose of dissolving a large amount of middle-low grade phosphate ores can be achieved.
Disclosure of Invention
The invention aims to solve the problems in the prior art and provides a method for heap leaching of medium and low grade phosphorite by combining a composite strain with waste biomass. The method utilizes cellulose degrading bacteria to decompose waste biomass, provides a directly utilized carbon source for heterotrophic phosphorus-dissolving bacteria, and then dissolves medium-and low-grade phosphate ores by means of the heterotrophic phosphorus-dissolving bacteria. The method has the advantages of strong processing capacity, environmental friendliness, low cost, simple process flow and the like. In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
a method for heap leaching of middle and low grade phosphorite by combining composite strains with waste biomass comprises the following steps: (a) collecting waste biomass and carrying out pretreatment to obtain waste biomass particles; (b) uniformly mixing phosphate rock powder and waste biomass particles and stacking; (c) and spraying the composite bacterial liquid containing the heterotrophic phosphate solubilizing bacteria and the cellulose degrading bacteria onto a mine pile for heap leaching.
Further, the pretreatment in the step (a) comprises crushing, alkali liquor soaking, washing and drying, and the waste biomass is selected from crop straws of rice, wheat, corn, soybean, rape, cotton and the like.
Furthermore, the waste biomass is firstly crushed into particles of 0.1mm-1mm, then is soaked in 2% -6% sodium hydroxide aqueous solution or potassium hydroxide aqueous solution for 8-10 days, and finally is sequentially filtered, washed and dried.
Furthermore, the particle size of the ground phosphate rock is 0.5mm-2mm, and the ground phosphate rock is formed by crushing medium and low grade phosphate rock with the phosphorus pentoxide mass content not more than 30%.
Further, when heaping is carried out in the step (b), the mass ratio of the ground phosphate rock to the waste biomass particles is 100: 0.5-5.
Furthermore, in the stacking process, the composite bacterial liquid is sprayed into the ore stack according to the mass ratio of 10-20%, so that the microbial flora can be fast colonized on the ground phosphate rock and the biomass particles.
Further, the ore pile is built in a flat and certain-gradient zone, and the bottom of the ore pile is provided with a bottom pad, a liquid collecting channel and a liquid collecting pool. Collecting the leachate through a liquid collecting channel and a liquid collecting pool, introducing air into the leachate at the speed of 20-50L/h for aeration and oxygen supplementation, and then conveying the leachate to the top of the ore heap for repeated spraying.
Further, the preparation method of the compound bacterial liquid in the step (c) is as follows: uniformly mixing the heterotrophic phosphorus-solubilizing bacteria liquid and the cellulose-degrading bacteria liquid according to the volume ratio of 1:1-3 to obtain a composite bacteria liquid; inoculating the composite bacterial liquid into a domestication culture medium containing phosphorus mineral powder, and subculturing and domesticating at 28-30 deg.C for 3-5 times until the total concentration of heterotrophic phosphorus-solubilizing bacteria and cellulose-degrading bacteria in the composite bacterial liquid is 106-108one/mL. The domestication culture medium comprises the following components: 5-10g/L glucose, 5-10g/L straw powder and 5-20g/L phosphate rock powder、2.5-5g/L MgCl2·7H2O、0.1-0.2g/L(NH4)2SO4、0.3-0.6g/L MgSO4·7H2O, 0.1-0.3g/L KCl and sterile water as a solvent.
Further, the heterotrophic phosphorus-solubilizing bacteria in the composite bacterial liquid in the step (c) are pseudomonas, and the cellulose-degrading bacteria are trichoderma asperellum.
Further, the separation and screening process of the heterotrophic phosphate solubilizing bacteria used for preparing the composite bacterial liquid is as follows: collecting a clover rhizosphere soil sample, uniformly mixing the clover rhizosphere soil sample with sterile water according to the proportion of 50-100g to 1L, and filtering to obtain a supernatant; mixing the supernatant with a phosphorus-solubilizing bacteria enrichment medium according to a weight ratio of 1:5-10, and performing constant temperature shaking culture at 28-30 ℃ and a rotation speed of 150-; inoculating the phosphorus-dissolving bacterium liquid obtained by enrichment in the previous step into a phosphorus-dissolving bacterium solid culture medium, and performing inverted culture at 28-30 ℃ for 3-5 days to obtain a mixed phosphorus-dissolving bacterium colony; selecting single colony, and performing purification culture at 28-30 deg.C for 3-5 days by streaking culture to obtain purified phosphorus-solubilizing bacteria strain; inoculating the purified phosphorus-dissolving bacterium strain into a phosphorus-dissolving bacterium screening culture medium, performing shake culture at 28-30 ℃ and a rotation speed of 150-.
The phosphate solubilizing bacteria enrichment medium comprises the following components: 8-10g/L glucose, 5-6g Ca3(PO4)2、2.5-5g/L MgCl2·7H2O、0.1-0.2g/L(NH4)2SO4、0.3-0.6g/L MgSO4·7H2O, 0.1-0.3g/L KCl and sterile water as a solvent. The phosphate solubilizing bacterium solid culture medium comprises the following components: 8-10g/L glucose, 5-6g Ca3(PO4)2、2.5-5g/L MgCl2·7H2O、0.1-0.2g/L(NH4)2SO4、0.3-0.6g/L MgSO4·7H2O, 0.1-0.3g/L KCl, 15-18g/L agar and sterile water as a solvent. The phosphate solubilizing bacteria screening culture medium comprises the following components: 8-10g/L glucose, 5-10g/L ground phosphate rock, 2.5-5g/L MgCl2·7H2O、0.1-0.2g/L(NH4)2SO4、0.3-0.6g/L MgSO4·7H2O, 0.1-0.3g/L KCl and sterile water as a solvent.
Further, the separation and screening process of the cellulose degrading bacteria used for preparing the composite bacterial liquid is as follows: collecting and crushing rotten wood samples, uniformly mixing the rotten wood samples with sterile water according to the proportion of 50-100g:1L, carrying out constant-temperature shaking culture at the temperature of 28-30 ℃ and the rotating speed of 150-; inoculating the cellulose degradation bacterium liquid prepared in the last step into a cellulose degradation bacterium solid culture medium, and performing inverted culture at 28-30 ℃ for 3-5 days to obtain a mixed cellulose degradation bacterium colony; selecting single colony, and performing purification culture at 28-30 deg.C for 3-5 days by streaking culture to obtain purified cellulose-degrading strain; inoculating the purified cellulose degrading strain into a cellulose degrading strain screening culture medium, performing inverted culture at 28-30 ℃ for 3-5 days, and screening out the cellulose degrading strain with a transparent ring; inoculating the screened cellulose degrading strain into a liquid enzyme production culture medium, carrying out shake culture for 5-7 days at the temperature of 28-30 ℃ and the rotating speed of 150-.
The cellulose degrading bacteria solid culture medium comprises the following components: 5-10g/L straw powder, 5-10g/L phosphate rock powder and 0.5-1g/L MgSO4·7H2O, 0.1-0.2g/L yeast extract powder, 0.001-0.002g/L CaCl2·2H2O, 15-18g/L agar, and sterile water as a solvent. The cellulose degrading bacteria screening culture medium comprises the following components: 5-10g/L sodium carboxymethylcellulose and 0.5-1g/L K2HPO4、0.25-0.5g/L MgSO40.2-0.25g/L Congo red, 15-18g/L agar and 2-3g/L gelatin, and the solvent is sterile water. The liquid enzyme production culture medium comprises the following components: 5-10g/L sodium carboxymethyl cellulose and 1-2g/L NH4Cl、0.5-1g/L MgSO4·7H2O、1-2g/L KH2PO41-2g/L of yeast extract powder, and sterile water as a solvent.
Further, the spraying speed of the compound bacterial liquid is 6.5-15L/(h.m)2) The spraying mode is intermittent and the spraying is carried out for 1 hour every 1-1.5 hours.
The waste biomass (such as straw, wheat straw, peanut straw and the like) used by the invention not only can provide a nidation point for microorganisms, but also can increase the ventilation quantity by virtue of a fluffy structure so as to be beneficial to the propagation of the microorganisms, and can decompose the waste biomass into nutrient substances which can be directly utilized by phosphorus-dissolving bacteria by virtue of the action of cellulose degrading bacteria, so that insoluble phosphorus is converted into soluble phosphorus by virtue of organic acid generated in the propagation process of the phosphorus-dissolving bacteria. The method for treating the medium-low grade phosphorite does not need mineral dressing enrichment, fully utilizes agricultural waste biomass, reduces the exogenous addition of nutrient substances, has the advantage of environmental protection while reducing the treatment cost, and can be used for subsequent phosphorus industry to obtain the solution with higher phosphorus content by circularly enriching the phosphorus-containing leachate.
The beneficial effects of the invention are shown in the following aspects: the problem of low-cost batch development and utilization of the medium-low grade phosphate ore is solved; waste biomass resources are fully utilized, so that the waste biomass resources are changed into valuable, and the concept of environmental friendliness and resource saving is met; the heap leaching method is simple to operate, easy to enlarge the size, low in investment and has industrial application value; the whole process flow is simple, the requirement on culture conditions is low, and a feasible method is provided for scientific utilization of rich middle-low grade phosphorite resources and recycling of waste biomass in China.
Drawings
FIG. 1 is a process flow diagram of the method of the present invention.
Preparing biomass, preparing ore, paving a bottom pad, mixing ground phosphate rock and biomass particles, stacking, culturing a composite strain, distributing liquid, collecting liquid, and spraying circularly.
Detailed Description
In order to make those skilled in the art fully understand the technical solutions and advantages of the present invention, the following embodiments are further described.
The heterotrophic phosphorus-solubilizing bacteria used in the invention are pseudomonas separated and screened from rhizosphere soil of clover, the cellulose degrading bacteria used in the invention are trichoderma asperellum separated and screened from rotten maple wood, and the specific process is as follows:
pseudomonas bacteria: collecting soil samples from roots of clover growing in a flower bed in Wuhan engineering university, uniformly mixing the collected soil samples with sterile water according to the proportion of 50-100g:1L, and filtering to obtain supernatant; mixing the supernatant with a phosphorus-solubilizing bacteria enrichment medium according to a weight ratio of 1:5-10, and performing constant temperature shaking culture at 28-30 ℃ and a rotation speed of 150-; inoculating the enriched phosphorus-dissolving bacterium liquid into a phosphorus-dissolving bacterium solid culture medium, and performing inverted culture at 28-30 ℃ for 3-5 days to obtain a mixed phosphorus-dissolving bacterium colony; selecting single colony, and performing purification culture at 28-30 deg.C for 3-5 days by streaking culture to obtain purified phosphorus-solubilizing bacteria strain; inoculating the purified phosphorus-dissolving bacterium strain into a phosphorus-dissolving bacterium screening culture medium, performing shake culture at 28-30 ℃ and a rotation speed of 150-. The phosphate solubilizing bacteria enrichment medium comprises the following components: 8-10g/L glucose, 5-6g Ca3(PO4)2、2.5-5g/L MgCl2·7H2O、0.1-0.2g/L(NH4)2SO4、0.3-0.6g/L MgSO4·7H2O, 0.1-0.3g/L KCl and sterile water as a solvent. The phosphorus-dissolving bacterium solid culture medium is added with agar components with the content of 15-18g/L on the basis of a phosphorus-dissolving bacterium enrichment culture medium; the phosphorus-solubilizing bacteria screening culture medium removes Ca on the basis of the phosphorus-solubilizing bacteria enrichment culture medium3(PO4)2The components are added, and the content of the ground phosphate rock is 5-10 g/L.
Trichoderma asperellum: collecting rotten wood samples from maple tree hearts growing in forests in Wuhan engineering university, crushing the collected rotten wood samples, uniformly mixing the crushed rotten wood samples with sterile water according to the proportion of 50-100g:1L, performing constant temperature shaking table culture at the temperature of 28-30 ℃ and the rotation speed of 150-170r/min for 0.5-3h, standing for 1-3h, and collecting supernatant to obtain cellulose degradation bacteria liquid; inoculating the cellulose-degrading bacteria liquid into a cellulose-degrading bacteria solid culture medium, and performing inverted culture at 28-30 ℃ for 3-5 days to obtain mixed cellulose-degrading bacteria colonies; selecting single colony, and performing purification culture at 28-30 deg.C for 3-5 days by streaking culture to obtain purified cellulose degrading strain; inoculating the purified cellulose degrading strain into a cellulose degrading strain screening culture medium, performing inverted culture at 28-30 ℃ for 3-5 days, and screening out the cellulose degrading strain with a transparent ring; inoculating the screened cellulose degrading strain into a liquid enzyme production culture medium, carrying out shake culture for 5-7 days at the temperature of 28-30 ℃ and the rotating speed of 150-.
The cellulose degrading bacteria solid culture medium comprises the following components: 5-10g/L straw powder, 5-10g/L phosphate rock powder and 0.5-1g/L MgSO4·7H2O, 0.1-0.2g/L yeast extract powder, 0.001-0.002g/L CaCl2·2H2O, 15-18g/L agar, and sterile water as a solvent. The cellulose degrading bacteria screening culture medium comprises the following components: 5-10g/L sodium carboxymethylcellulose and 0.5-1g/L K2HPO4、0.25-0.5g/L MgSO40.2-0.25g/L Congo red, 15-18g/L agar and 2-3g/L gelatin, and the solvent is sterile water. The liquid enzyme production culture medium comprises the following components: 5-10g/L sodium carboxymethyl cellulose and 1-2g/L NH4Cl、0.5-1g/L MgSO4·7H2O、1-2g/L KH2PO41-2g/L of yeast extract powder, and sterile water as a solvent.
The phosphate rock powder and the straw powder used in the culture medium and the subsequent culture medium can be directly prepared into raw materials for building and piling, and the particle size is further reduced. For example, the particle size of the ground phosphate rock is controlled within the range of 0.075-0.15mm, and the mass content of the phosphorus pentoxide is controlled within 20-30%; the grain diameter of the straw powder is controlled to be 0.9 mm.
The preparation method of the compound bacterial liquid comprises the following steps: the separated and screened pseudomonas bacteria liquid and trichoderma asperellum bacteria liquid are uniformly mixed according to the volume ratio of 1:1-3, and the composite bacteria liquid prepared by the method can be used as a spray liquid only by domestication before use.
Example 1
a) Preparation of biomass: 0.05kg of straw stalk was weighed and crushed into 0.5mm pellets. Adding straw particles into a NaOH aqueous solution with the mass fraction of 2%, soaking for 8 days, filtering by using double-layer gauze after the time is up, washing until the pH value of a supernatant is neutral, and drying filter residues at 50 ℃ until the weight is constant for later use.
b) Preparing ore: weighing 1kg of phosphate ore (the phosphorus pentoxide mass content is 23.75%), and crushing the phosphate ore to 1mm to obtain phosphate ore powder.
c) D, bedding and bottom padding: and paving a high-strength polyethylene sheet at the selected position of the heap to serve as a heap leaching bottom pad, and digging liquid collecting ditches and a liquid collecting pool at the bottom and the periphery of the heap. The heap should be selected in a flat zone requiring a grade (3% longitudinal slope) in the field so that leachate collects out, but not too great to prevent the mat from sliding. Size of heap ore: the length of the storage yard is 0.24m, the width is 0.2m, the height is 0.08m, and the height of the single-layer stack is 0.02 m.
d) Mixing: uniformly mixing the phosphate rock powder in the step b) with the rice straw particles pretreated in the step a) to obtain mixed powder.
e) Building a pile: and (3) piling by using the mixed powder as a raw material and adopting a three-layer piling method, and spraying the domesticated composite bacterial liquid according to the mass ratio of 10% during piling (the domestication method is shown in step f), so that the microorganisms are ensured to be colonized on the ground phosphate rock and the biomass, and meanwhile, the piling is also facilitated.
f) Culturing the compound strain: subculturing the composite bacterial liquid for 3 times by using an acclimatization culture medium containing phosphorus mineral powder and straw powder before building and stacking the liquid to finish acclimatization, and gradually performing amplification culture on the acclimatized composite bacterial strain until the number of the two microorganisms reaches 107one/mL, thus obtaining a spray solution. The formula of the domestication culture medium is specifically as follows (the same below): 10g/L glucose, 7g/L pretreated biomass, 10g/L ground phosphate rock, 5g/L MgCl2·7H2O、0.1g/L(NH4)2SO4、0.25g/L MgSO4·7H2O, 0.2g/L KCl and sterile water as a solvent.
g) Liquid distribution: the spraying liquid is sent to the heap leaching field through the main liquid conveying pipe, and then the liquid is distributed to each ore heap through the branch pipeline system. Spraying the spraying liquid on the surface of the ore heap by small holes or nozzles uniformly distributed on the branch pipes, and distributing the liquid in an intermittent spraying mode, wherein the spraying is stopped for 1.5 hours every time the spraying is carried out for 1 hour, and the spraying intensity is 10L/(h.m)2)。
h) Liquid collection: and redundant spraying liquid flows into the liquid collecting pool through the liquid collecting ditch to form leaching liquid, and the leaching liquid is subjected to aeration and oxygen supplementation treatment at an aeration rate of 20L/h.
i) Circulating spraying: pumping the leachate after aeration and oxygen supplementation to a heap leaching field again for spraying.
And (3) observing, sampling and detecting in the heap leaching process, basically completing the heap leaching after 9 months, and calculating to find that the leaching rate of phosphorus in the medium and low grade phosphorite reaches 58.82%.
Example 2
a) Preparation of biomass: 0.1kg of rice straw was weighed and crushed into 0.1mm pellets. Adding straw particles into a NaOH aqueous solution with the mass fraction of 2%, soaking for 8 days, filtering by using double-layer gauze after the time is up, washing until the pH value of a supernatant is neutral, and drying filter residues at 50 ℃ until the weight is constant for later use.
b) Preparing ore: 2kg of phosphate ore (the phosphorus pentoxide content is 23.75 percent by mass) is weighed and crushed to 1mm to obtain phosphate ore powder.
c) D, bedding and bottom padding: and paving a high-strength polyethylene sheet at the selected position of the heap to serve as a heap leaching bottom pad, and digging liquid collecting ditches and a liquid collecting pool at the bottom and the periphery of the heap. Heap leaching site selection and setup requirements were the same as in example 1.
d) Mixing: uniformly mixing the phosphate rock powder in the step b) with the rice straw particles pretreated in the step a) to obtain mixed powder.
e) Building a pile: and (3) piling by using the mixed powder as a raw material by adopting a three-layer piling method, and spraying the domesticated composite bacterial liquid according to a mass ratio of 15% during piling. Size of heap ore: the length of the storage yard is 0.24m, the width is 0.2m, the height is 0.13m, and the height of the single-layer stack is 0.03 m.
f) Culturing the compound strain: subculturing the composite bacterial liquid for 3 times by using a domestication culture medium before building a stacking liquid to complete domestication, and gradually performing amplification culture on the domesticated composite bacterial strain until the number of the two microorganisms reaches 108one/mL, thus obtaining a spray solution.
g) Liquid distribution: the spraying liquid is sent to the heap leaching field through the main liquid conveying pipe, and then the liquid is distributed to each ore heap through the branch pipeline system. Is evenly dividedSpraying the spraying liquid on the surface of the ore heap by small holes or nozzles distributed on the branch pipes, and distributing the liquid in an intermittent spraying mode, wherein the spraying is stopped for 1h every time the spraying is stopped for 1h, and the spraying strength is 6.5L/(h.m)2)。
h) Liquid collection: and redundant spraying liquid flows into the liquid collecting pool through the liquid collecting ditch to form leaching liquid, and the leaching liquid is subjected to inflation and oxygen supplementation treatment at an inflation rate of 50L/h.
i) Circulating spraying: pumping the leachate after the aeration and oxygen supplementation treatment to a heap leaching field again for spraying.
The whole process is shown in figure 1, the phosphorus content in the phosphate rock is sampled and detected after 9 months of heap leaching, and the leaching rate of the phosphorus in the medium and low grade phosphate rock is found to reach 57.21 percent by calculation.
Example 3
a) Preparation of biomass: 0.15kg of rice straw was weighed and crushed into 1mm pellets. Adding straw particles into a NaOH aqueous solution with the mass fraction of 2%, soaking for 8 days, filtering by using double-layer gauze after the time is up, washing until the pH value of a supernatant is neutral, and drying filter residues at 50 ℃ until the weight is constant for later use.
b) Preparing ore: 3kg of phosphate ore (the phosphorus pentoxide content is 23.75 percent by mass) is weighed and crushed to 1mm to obtain phosphate ore powder.
c) D, bedding and bottom padding: and paving a high-strength polyethylene sheet at the selected position of the heap to serve as a heap leaching bottom pad, and digging liquid collecting ditches and a liquid collecting pool at the bottom and the periphery of the heap. Heap leaching site selection and setup requirements were the same as in example 1.
d) Mixing: uniformly mixing the phosphate rock powder in the step b) with the rice straw particles pretreated in the step a) to obtain mixed powder.
e) Building a pile: and (3) piling by using the mixed powder as a raw material by adopting a three-layer piling method, and spraying the domesticated composite bacterial liquid according to the mass ratio of 20% during piling. Size of heap ore: the length is 0.24m, the width is 0.2m, the height is 0.18m, and the single-layer stack height is 0.05 m.
f) Culturing the compound strain: subculturing the composite bacterial liquid for 5 times by using a domestication culture medium before building a stacking liquid to complete domestication, and gradually performing amplification culture on the domesticated composite bacterial strain until the number of the two microorganisms reaches 108one/mL, thus obtaining a spray solution.
g) Liquid distribution: the spraying liquid is sent to the heap leaching field through the main liquid conveying pipe, and then the liquid is distributed to each ore heap through the branch pipeline system. Spraying the spraying liquid on the surface of the ore heap by small holes or nozzles uniformly distributed on the branch pipes, and distributing the liquid in an intermittent spraying mode, wherein the spraying is stopped for 1.5 hours every time the spraying is carried out for 1 hour, and the spraying intensity is 15L/(h.m)2)。
h) Liquid collection: and redundant spraying liquid flows into the liquid collecting pool through the liquid collecting ditch to form leaching liquid, and the leaching liquid is subjected to aeration and oxygen supplementation treatment at an aeration rate of 20L/h.
i) Circulating spraying: pumping the leachate after the aeration and oxygen supplementation treatment to a heap leaching field again for spraying.
Sampling and detecting the phosphorus content in the phosphate rock after 9 months of heap leaching, and calculating to find that the leaching rate of the phosphorus in the medium and low grade phosphate rock reaches 55.54 percent.
Example 4
a) Preparation of biomass: 1kg of straw stalk was weighed and crushed into 0.7mm pellets. Adding straw particles into a NaOH aqueous solution with the mass fraction of 2%, soaking for 8 days, filtering by using double-layer gauze after the time is up, washing until the pH value of a supernatant is neutral, and drying filter residues at 50 ℃ until the weight is constant for later use.
b) Preparing ore: 10kg of phosphate ore (the phosphorus pentoxide content is 23.75 percent by mass) is weighed and crushed to 1mm to obtain phosphate ore powder.
c) D, bedding and bottom padding: and paving a high-strength polyethylene sheet at the selected position of the heap to serve as a heap leaching bottom pad, and digging liquid collecting ditches and a liquid collecting pool at the bottom and the periphery of the heap. Heap leaching site selection and setup requirements were the same as in example 1.
d) Mixing: uniformly mixing the phosphate rock powder in the step b) with the rice straw particles pretreated in the step a) to obtain mixed powder.
e) Building a pile: piling the mixed powder serving as a raw material into a trapezoidal pile, and spraying the domesticated composite bacterial liquid according to the mass ratio of 10% during piling. Size of heap ore: the length of the upper bottom of the storage yard is 0.27m, the width of the upper bottom is 0.24m, the length of the lower bottom is 0.49m, the width of the lower bottom is 0.42m, and the height is 0.18 m.
f) Culturing the compound strain: buildingSubculturing the composite bacterial liquid for 4 times by using an acclimatization culture medium before stacking the liquid to finish acclimatization, and gradually performing amplification culture on the acclimatized composite bacterial strain until the number of the two microorganisms reaches 108one/mL, thus obtaining a spray solution.
g) Liquid distribution: the spraying liquid is sent to the heap leaching field through the main liquid conveying pipe, and then the liquid is distributed to each ore heap through the branch pipeline system. Spraying the spraying liquid on the surface of the ore heap by small holes or nozzles uniformly distributed on the branch pipes, distributing the liquid in an intermittent spraying mode, stopping spraying for 1 hour every time the spraying is carried out for 1 hour, and spraying strength is 6.5L/(h.m)2)。
h) Liquid collection: and redundant spraying liquid flows into the liquid collecting pool through the liquid collecting ditch to form leaching liquid, and the leaching liquid is subjected to aeration and oxygen supplementation treatment at an aeration rate of 30L/h.
i) Circulating spraying: pumping the leachate after the aeration and oxygen supplementation treatment to a heap leaching field again for spraying.
Sampling and detecting the phosphorus content in the phosphate rock after 9 months of heap leaching, and calculating to find that the leaching rate of the phosphorus in the medium and low grade phosphate rock reaches 47.19 percent.
Example 5
a) Preparation of biomass: weighing 1kg of wheat straw, and crushing into 1mm particles. Adding the wheat straw particles into a NaOH aqueous solution with the mass fraction of 2%, soaking for 8 days, filtering by using double-layer gauze after the time, washing until the pH value of a supernatant is neutral, and drying filter residues at 50 ℃ until the weight is constant for later use.
b) Preparing ore: 10kg of phosphate ore (the phosphorus pentoxide content is 23.75 percent by mass) is weighed and crushed to 1mm to obtain phosphate ore powder.
c) D, bedding and bottom padding: and paving a high-strength polyethylene sheet at the selected position of the heap to serve as a heap leaching bottom pad, and digging liquid collecting ditches and a liquid collecting pool at the bottom and the periphery of the heap. Heap leaching site selection and setup requirements were the same as in example 1.
d) Mixing: uniformly mixing the phosphate rock powder in the step b) with the wheat straw particles pretreated in the step a) to obtain mixed powder.
e) Building a pile: piling the mixed powder serving as a raw material into a trapezoidal pile, and spraying the domesticated composite bacterial liquid according to the mass ratio of 10% during piling. Size of heap ore: the length of the upper bottom of the storage yard is 0.27m, the width of the upper bottom is 0.24m, the length of the lower bottom is 0.49m, the width of the lower bottom is 0.42m, and the height is 0.18 m.
f) Culturing the compound strain: subculturing the composite bacterial liquid for 5 times by using a domestication culture medium before building a stacking liquid to complete domestication, and gradually performing amplification culture on the domesticated composite bacterial strain until the number of the two microorganisms reaches 106one/mL, thus obtaining a spray solution.
g) Liquid distribution: the spraying liquid is sent to the heap leaching field through the main liquid conveying pipe, and then the liquid is distributed to each ore heap through the branch pipeline system. Spraying the spraying liquid on the surface of the ore heap by small holes or nozzles uniformly distributed on the branch pipes, and distributing the liquid in an intermittent spraying mode, wherein the spraying is stopped for 1.3 hours every time the spraying is carried out for 1 hour, and the spraying intensity is 12L/(h.m)2)。
h) Liquid collection: and redundant spraying liquid flows into the liquid collecting pool through the liquid collecting ditch to form leaching liquid, and the leaching liquid is subjected to inflation and oxygen supplementation treatment at the inflation rate of 28L/h.
i) Circulating spraying: pumping the leachate after the aeration and oxygen supplementation treatment to a heap leaching field again for spraying.
Sampling and detecting the phosphorus content in the phosphate rock after 9 months of heap leaching, and calculating to find that the leaching rate of the phosphorus in the medium and low grade phosphate rock reaches 43.09%.
Example 6
a) Preparation of biomass: weighing 1kg of corn straw, and crushing into 0.1mm particles. Adding the corn straw particles into a NaOH aqueous solution with the mass fraction of 2%, soaking for 8 days, filtering by using double-layer gauze after the time, washing until the pH value of a supernatant is neutral, and drying filter residues at 50 ℃ until the weight is constant for later use.
b) Preparing ore: 10kg of phosphate ore (the phosphorus pentoxide content is 23.75 percent by mass) is weighed and crushed to 1mm to obtain phosphate ore powder.
c) D, bedding and bottom padding: and paving a high-strength polyethylene sheet at the selected position of the heap to serve as a heap leaching bottom pad, and digging liquid collecting ditches and a liquid collecting pool at the bottom and the periphery of the heap. Heap leaching site selection and setup requirements were the same as in example 1.
d) Mixing: uniformly mixing the phosphate rock powder in the step b) with the corn straw particles pretreated in the step a) to obtain mixed powder.
e) Building a pile: piling the mixed powder serving as a raw material into a trapezoidal pile, and spraying the domesticated composite bacterial liquid according to the mass ratio of 10% during piling. Size of heap ore: the upper bottom length is 0.27m, the upper bottom width is 0.24m, the lower bottom length is 0.49m, the lower bottom width is 0.42m, and the height is 0.18 m.
f) Culturing the compound strain: subculturing the composite bacterial liquid for 5 times by using a domestication culture medium before building a stacking liquid to complete domestication, and gradually performing amplification culture on the domesticated composite bacterial strain until the number of the two microorganisms reaches 108one/mL, thus obtaining a spray solution.
g) Liquid distribution: the spraying liquid is sent to the heap leaching field through the main liquid conveying pipe, and then the liquid is distributed to each ore heap through the branch pipeline system. Spraying the spraying liquid on the surface of the ore heap by small holes or nozzles uniformly distributed on the branch pipes, and distributing the liquid in an intermittent spraying mode, wherein the spraying is stopped for 1.2 hours every time the spraying is carried out for 1 hour, and the spraying intensity is 14L/(h.m)2)。
h) Liquid collection: and redundant spraying liquid flows into the liquid collecting pool through the liquid collecting ditch to form leaching liquid, and the leaching liquid is subjected to aeration and oxygen supplementation treatment at an aeration rate of 20L/h.
i) Circulating spraying: pumping the leachate after the aeration and oxygen supplementation treatment to a heap leaching field again for spraying.
Sampling and detecting the phosphorus content in the phosphate rock after 9 months of heap leaching, and calculating to find that the leaching rate of the phosphorus in the medium and low grade phosphate rock reaches 48.24 percent.
Claims (5)
1. A method for heap leaching of middle and low grade phosphorite by combining a composite strain with waste biomass is characterized by comprising the following steps:
(a) collecting waste biomass and carrying out pretreatment to obtain waste biomass particles;
(b) uniformly mixing phosphate rock powder and waste biomass particles, and stacking, wherein in the stacking process, a composite bacterial liquid containing heterotrophic phosphorus-solubilizing bacteria and cellulose-degrading bacteria is sprayed into a ore pile according to the mass ratio of 10-20%;
(c) spraying a composite bacterial liquid containing heterotrophic phosphate solubilizing bacteria and cellulose degrading bacteria onto a mine pile for heap leaching;
the preparation method of the compound bacterial liquid comprises the following steps: uniformly mixing the heterotrophic phosphorus-solubilizing bacteria liquid and the cellulose-degrading bacteria liquid according to the volume ratio of 1:1-3, inoculating the obtained mixture into a domestication culture medium containing phosphorus mineral powder, and carrying out subculture domestication culture for 3-5 times at 28-30 ℃ until the total concentration of the heterotrophic phosphorus-solubilizing bacteria and the cellulose-degrading bacteria in the bacteria liquid is 106-108Per mL; the domestication culture medium comprises the following components: 5-10g/L glucose, 5-10g/L straw powder, 5-20g/L phosphate rock powder and 2.5-5g/L MgCl2·7H2O、0.1-0.2g/L (NH4)2SO4、0.3-0.6g/L MgSO4·7H2O, 0.1-0.3g/L KCl and sterile water as a solvent;
the heterotrophic phosphate solubilizing bacteria are pseudomonas separated and screened from soil, and the separation and screening process comprises the following steps: collecting a clover rhizosphere soil sample, uniformly mixing the clover rhizosphere soil sample with sterile water according to the proportion of 50-100g to 1L, and filtering to obtain a supernatant; mixing the supernatant with a phosphorus-solubilizing bacteria enrichment medium according to a weight ratio of 1:5-10, and performing constant temperature shaking culture at 28-30 ℃ and a rotation speed of 150-; inoculating the phosphorus-dissolving bacterium liquid obtained by enrichment in the previous step into a phosphorus-dissolving bacterium solid culture medium, and performing inverted culture at 28-30 ℃ for 3-5 days to obtain a mixed phosphorus-dissolving bacterium colony; selecting single colony, and performing purification culture at 28-30 deg.C for 3-5 days by streaking culture to obtain purified phosphorus-solubilizing bacteria strain; inoculating the purified phosphorus-dissolving bacterium strain into a phosphorus-dissolving bacterium screening culture medium, performing shake culture at 28-30 ℃ and a rotating speed of 150-; the phosphate solubilizing bacteria enrichment medium comprises the following components: 8-10g/L glucose, 5-6g/L Ca3(PO4)2、2.5-5g/L MgCl2·7H2O、0.1-0.2g/L (NH4)2SO4、0.3-0.6g/L MgSO4·7H2O, 0.1-0.3g/L KCl and sterile water as a solvent; the phosphate solubilizing bacterium solid culture medium comprises the following components: 8-10g/L glucose, 5-6g/L Ca3(PO4)2、2.5-5g/L MgCl2·7H2O、0.1-0.2g/L (NH4)2SO4、0.3-0.6g/L MgSO4·7H2O, 0.1-0.3g/L KCl, 15-18g/L agar and sterile water as a solvent; the phosphate solubilizing bacteria screening culture medium comprises the following components: 8-10g/L glucose, 5-10g/L ground phosphate rock, 2.5-5g/L MgCl2·7H2O、0.1-0.2g/L (NH4)2SO4、0.3-0.6g/L MgSO4·7H2O, 0.1-0.3g/L KCl and sterile water as a solvent;
the cellulose degrading bacteria are trichoderma asperellum separated and screened from rotten wood, and the separating and screening process is as follows: collecting and crushing rotten wood samples, uniformly mixing the rotten wood samples with sterile water according to the proportion of 50-100g:1L, carrying out constant-temperature shaking culture at the temperature of 28-30 ℃ and the rotating speed of 150-; inoculating the cellulose degradation bacterium liquid prepared in the last step into a cellulose degradation bacterium solid culture medium, and performing inverted culture at 28-30 ℃ for 3-5 days to obtain a mixed cellulose degradation bacterium colony; selecting single colony, and performing purification culture at 28-30 deg.C for 3-5 days by streaking culture to obtain purified cellulose-degrading strain; inoculating the purified cellulose degrading strain into a cellulose degrading strain screening culture medium, performing inverted culture at 28-30 ℃ for 3-5 days, and screening out the cellulose degrading strain with a transparent ring; inoculating the screened cellulose degrading bacteria strain into a liquid enzyme production culture medium, carrying out shake culture for 5-7 days at the temperature of 28-30 ℃ and the rotating speed of 150-; the cellulose degrading bacteria solid culture medium comprises the following components: 5-10g/L straw powder, 5-10g/L phosphate rock powder and 0.5-1g/L MgSO4·7H2O, 0.1-0.2g/L yeast extract powder, 0.001-0.002g/L CaCl2·2H2O, 15-18g/L agar, and sterile water as a solvent; the cellulose degrading bacteria screening culture medium comprises the following components: 5-10g/L sodium carboxymethylcellulose and 0.5-1g/L K2HPO4、0.25-0.5g/L MgSO40.2-0.25g/L Congo red, 15-18g/L agar and 2-3g/L gelatin, and the solvent is sterile water; the liquid enzyme production culture medium comprises the following components: 5-10g/L sodium carboxymethyl cellulose and 1-2g/L NH4Cl、0.5-1g/L MgSO4·7H2O、1-2g/L KH2PO41-2g/L of yeast extract powder, and sterile water as a solvent.
2. The method of claim 1, wherein: the pretreatment in the step (a) comprises crushing, alkali liquor soaking, washing and drying, and the waste biomass is selected from rice straws, wheat straws, corn straws, soybean straws, rape straws and cotton straws.
3. The method of claim 1, wherein: the phosphate rock powder is formed by crushing medium and low grade phosphate rock with the phosphorus pentoxide mass content not more than 30%, the particle sizes of the phosphate rock powder and the waste biomass particles are 0.5-2 mm and 0.1-1 mm respectively, and the mass ratio of the phosphate rock powder to the waste biomass particles is 100:0.5-5 when the phosphate rock powder and the waste biomass particles are mixed and piled.
4. The method of claim 1, wherein: the method comprises the following steps of building a mine pile in a flat and certain-gradient zone, arranging a bottom pad, a liquid collecting channel and a liquid collecting pool at the bottom of the mine pile, collecting leachate through the liquid collecting channel and the liquid collecting pool, introducing air into the leachate at a speed of 20-50L/h for aeration and oxygen supplementation, and then conveying the leachate to the top of the mine pile for repeated spraying.
5. The method of claim 1, wherein: the spraying speed of the composite bacterial liquid is 6.5-15L/(h.m)2) The spraying mode is intermittent and the spraying is carried out for 1 hour every 1-1.5 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911328294.XA CN111069237B (en) | 2019-12-20 | 2019-12-20 | Medium-low grade phosphorite heap leaching method combining composite strain with waste biomass |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911328294.XA CN111069237B (en) | 2019-12-20 | 2019-12-20 | Medium-low grade phosphorite heap leaching method combining composite strain with waste biomass |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111069237A CN111069237A (en) | 2020-04-28 |
| CN111069237B true CN111069237B (en) | 2021-07-13 |
Family
ID=70316402
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911328294.XA Active CN111069237B (en) | 2019-12-20 | 2019-12-20 | Medium-low grade phosphorite heap leaching method combining composite strain with waste biomass |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111069237B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111979159A (en) * | 2020-09-03 | 2020-11-24 | 中南大学 | Phosphate solubilizing bacterium agent and preparation method and application thereof |
| CN114934176B (en) * | 2022-05-06 | 2023-02-17 | 北京科技大学 | System and method for reinforcing copper sulfide ore biological heap leaching by using intermittent liquid distribution |
| CN115500238B (en) * | 2022-09-15 | 2024-01-09 | 湖北三峡实验室 | Method for improving phosphogypsum storage yard soil by using waste straw |
| CN115537208A (en) * | 2022-09-30 | 2022-12-30 | 武汉工程大学 | Method for preparing soil conditioner by utilizing phosphorus potassium tailings and waste biomass |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434917A (en) * | 2007-11-13 | 2009-05-20 | 北京有色金属研究总院 | Acidophilic leaching-ore bacteria, heap bioleaching process for low grade phosphate ore by using the same |
| CN101838737A (en) * | 2009-12-01 | 2010-09-22 | 西部矿业股份有限公司 | Method for leaching low-grade phosphate ore from ferrous oxide thiobacillus |
| CN101891166A (en) * | 2009-12-01 | 2010-11-24 | 西部矿业股份有限公司 | Method for leaching low-grade phosphorite with mixed bacillus of thiobacillus thioxidans and thiobacillus ferrooxidans |
| CN102102085A (en) * | 2009-12-18 | 2011-06-22 | 北京有色金属研究总院 | Acidithiobacillus thiooxidans and heap leaching technology using same of middle-grade and low-grade phosphate ore |
| CN102173399A (en) * | 2011-03-08 | 2011-09-07 | 武汉工程大学 | Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile |
| CN102432363A (en) * | 2011-09-09 | 2012-05-02 | 西南科技大学 | Method for preparing bacterial composite phosphate fertilizer with medium and low grade phosphate ores |
| CN103992958A (en) * | 2014-05-27 | 2014-08-20 | 南京农业大学 | Rice straw degrading fungi trichoderma koningiopsis ZJC-1 and fungicide thereof |
| CN106276833A (en) * | 2016-07-29 | 2017-01-04 | 深圳市芭田生态工程股份有限公司 | The bioleaching process of insoluble phosphate |
| CN108486017A (en) * | 2018-04-27 | 2018-09-04 | 苏州大学 | A kind of garden waste degradation bacterial agent |
-
2019
- 2019-12-20 CN CN201911328294.XA patent/CN111069237B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101434917A (en) * | 2007-11-13 | 2009-05-20 | 北京有色金属研究总院 | Acidophilic leaching-ore bacteria, heap bioleaching process for low grade phosphate ore by using the same |
| CN101838737A (en) * | 2009-12-01 | 2010-09-22 | 西部矿业股份有限公司 | Method for leaching low-grade phosphate ore from ferrous oxide thiobacillus |
| CN101891166A (en) * | 2009-12-01 | 2010-11-24 | 西部矿业股份有限公司 | Method for leaching low-grade phosphorite with mixed bacillus of thiobacillus thioxidans and thiobacillus ferrooxidans |
| CN102102085A (en) * | 2009-12-18 | 2011-06-22 | 北京有色金属研究总院 | Acidithiobacillus thiooxidans and heap leaching technology using same of middle-grade and low-grade phosphate ore |
| CN102173399A (en) * | 2011-03-08 | 2011-09-07 | 武汉工程大学 | Method for dissolving mid-low-grade rock phosphate by utilizing thermoacidophile |
| CN102432363A (en) * | 2011-09-09 | 2012-05-02 | 西南科技大学 | Method for preparing bacterial composite phosphate fertilizer with medium and low grade phosphate ores |
| CN103992958A (en) * | 2014-05-27 | 2014-08-20 | 南京农业大学 | Rice straw degrading fungi trichoderma koningiopsis ZJC-1 and fungicide thereof |
| CN106276833A (en) * | 2016-07-29 | 2017-01-04 | 深圳市芭田生态工程股份有限公司 | The bioleaching process of insoluble phosphate |
| CN108486017A (en) * | 2018-04-27 | 2018-09-04 | 苏州大学 | A kind of garden waste degradation bacterial agent |
Non-Patent Citations (2)
| Title |
|---|
| 中低品位磷矿微生物溶解基础研究;肖春桥;《农业科技辑》;20090531;第70页 * |
| 纤维素分解菌与无机磷细菌的相互作用;林启美;《生态学杂质》;20010331;第69-70页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111069237A (en) | 2020-04-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111069237B (en) | Medium-low grade phosphorite heap leaching method combining composite strain with waste biomass | |
| CN111011159B (en) | Preparation method of ecological improvement matrix based on coal gangue and sludge | |
| CN111019865B (en) | Pseudomonas graminis strain capable of degrading cellulose at low temperature and application thereof | |
| CN106187341B (en) | Cultivate excreta three grade fermemtation method | |
| CN112337033B (en) | Harmless treatment method of abamectin slag, product and application of product as sandy soil modifier | |
| CN102586110A (en) | Facultative anaerobe microbial preparation, its preparation method and application in straw degrading | |
| CN105695367A (en) | Compound fungicide FX for degrading straws and application thereof | |
| CN103014336B (en) | Preparation and method for treating high-arsenic and high-sulfur gold ore | |
| CN103031434B (en) | Refractory gold ore desulfurizing and dearsenifying method | |
| CN106811422A (en) | Microbial inoculum cultural method and the technique for carrying out stalk fermentation pretreatment using the microbial inoculum | |
| CN113215201A (en) | Coupling process for mixing pig manure with rice straw solid-state biogas fermentation and biogas residue aerobic composting | |
| CN113355099B (en) | Phosphogypsum soil conditioner and preparation method thereof | |
| CN101289339A (en) | Multiple organic humic acid spraying liquid fertilizer and preparation method | |
| CN105503295A (en) | Special culture medium for blueberry and production process thereof | |
| CN114223343A (en) | Ecological management method for rare earth tailings restoration | |
| CN103992958B (en) | One strain rice straw degradative fungi intends healthy and free from worry Trichoderma spp. ZJC-1 and microbial inoculum thereof | |
| CN103088222B (en) | Process for treating refractory gold ores | |
| CN113024310A (en) | Novel bio-organic fertilizer and preparation method thereof | |
| WO2014032314A1 (en) | Palm oil byproduct biological humic acid, production method thereof and biological humic acid conversion agent used in the method | |
| CN110042063B (en) | Gliocladium roseum (Clinostacchys rosea) strain YZC3 and application thereof | |
| CN110835610B (en) | Composite microbial inoculum suitable for degrading straw and preparation method thereof | |
| CN114507614A (en) | Bacillus for degrading straw and inhibiting plant pathogenic fungi and application thereof | |
| CN110093296B (en) | Filter mud high-temperature composting microbial inoculum LC and application thereof | |
| CN103074489B (en) | Processing method of refractory gold ore | |
| CN115368906B (en) | Goethite immobilized iron-sulfur reducing bacteria composite material and preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |