CN110042063B - Gliocladium roseum (Clinostacchys rosea) strain YZC3 and application thereof - Google Patents
Gliocladium roseum (Clinostacchys rosea) strain YZC3 and application thereof Download PDFInfo
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Abstract
The invention discloses a gliocladium roseum (Clinostasys rosea) strain YZC3 and application thereof, belonging to the technical field of microorganisms. The preservation number of the gliocladium roseum strain YZC3 is CGMCC No. 14782. Based on the gliocladium roseum strain YZC3, the invention also provides a product capable of degrading lignin, a microbial inoculum for degrading lignin, a product for landscaping waste compost, a microbial inoculum for landscaping waste compost and a method for landscaping waste compost. The gliocladium roseum strain YZC3 of the present invention has an excellent effect in degrading lignin.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a gliocladium roseum (Clonostachys rosea) strain YZC3 and application thereof.
Background
Biomass energy is an important renewable energy source, and lignin is an important component of biomass and has been receiving attention. From the beginning of the 20 th century, some experts and scholars at home and abroad began to explore the best way to degrade lignin. The related research methods mainly comprise physical methods, chemical methods, biological methods and the like. Among them, the biodegradation method is concerned about because of its advantages of mild action condition, strong specificity, environmental protection, low cost, etc. Lignin is a biopolymer widely present in biomass and has a monomeric structure of phenylpropane. The lignin in the garden waste can be directly discarded in the modes of straw burning or pulping waste water discharge, or directly discarded in a stacking mode, so that the waste of biomass resources is caused. In the recycling application of lignin, the lignin can be converted into small molecular substances through biodegradation, and then the small molecular substances are separated and purified into chemical raw materials. The method has the advantages of low cost, small secondary pollution and the like, and is more favored compared with physical and chemical methods. The lignin has a complex and stable structure and is difficult to biodegrade, so the degrading capability of microorganisms is a decisive factor.
Practice shows that microbial degradation of lignin is the best way, and the method has the advantages of high degradation rate, safety, environmental protection, low cost, reproducibility and the like. Meanwhile, a large number of microorganisms capable of degrading lignin exist in nature, including fungi, bacteria and actinomycetes, and mainly consist of microorganisms from soil and animal digestive tracts.
For example, patent application 201711207383.X provides a Myrothecium verrucaria mutant strain T2901 with the preservation number of CCTCC NO: M2017413, and the lignin degradation rate of the strain can reach 42.30%.
Patent application 201711202906.1 discloses a composite microbial inoculum for degrading corn stalks, which contains Aspergillus niger (Aspergillus), Trichoderma (Trichoderma), Penicillium oxalicum (Penicillium), Phanerochaete chrysosporium (Phanerochaete chrysosporium) as active ingredients; or Aspergillus niger (Aspergillus) fermentation liquor, Trichoderma (Trichoderma) fermentation liquor, Penicillium oxalicum (Penicillium) fermentation liquor and Phanerochaete chrysosporium (Phanerochaete chrysosporium) fermentation liquor, wherein the lignin degradation rate of the composite microbial inoculum is 28.5%.
The field needs to screen and develop more new strains with better lignin degradation effect for selection and use in production practice.
Disclosure of Invention
Based on the needs in the field, the invention extracts and screens a gliocladium roseum (Clonostachys rosea) strain YZC3 from a soil sample, and the strain is identified to be capable of effectively degrading lignin and being applied to garden waste compost.
The technical scheme of the invention is as follows:
a Gliocladium roseum (Clinostacchys rosea) strain YZC3 with preservation number of CGMCC No. 14782.
A lignin-degradable article characterized in that the active ingredient of said article comprises the strain gliocladium roseum (Clonostachys rosea) YZC3 of claim 1.
A microbial agent for degrading lignin, comprising an active ingredient for degrading lignin; characterized in that the active ingredients comprise the gliocladium roseum (Clonostachys rosea) strain YZC3 of claim 1;
preferably, the microbial inoculum also comprises auxiliary materials commonly used for preparing microbial inoculants.
A product for composting landscaping waste characterized in that the active ingredient of the product comprises the scopularia rosea (Clonostachys rosea) strain YZC3 of claim 1.
The microbial inoculum for the garden greening waste compost comprises active ingredients; characterized in that the active ingredients comprise the gliocladium roseum (Clonostachys rosea) strain YZC3 of claim 1;
preferably, the microbial inoculum also comprises auxiliary materials commonly used for preparing microbial inoculants.
A method for composting landscaping waste, comprising: use of the gliocladium roseum (Clonostachys rosea) strain YZC3 of claim 1 in composting processes.
The method also comprises the conventional steps of composting landscaping waste;
preferably, the composting temperature is above 50 ℃ and is maintained for 5-7 d.
Compost, which is characterized by being prepared by adding the gliocladium roseum (Clinostasys rosea) strain YZC3 into compost materials and/or inoculating the gliocladium roseum strain YZC 3.
The compost material comprises landscaping waste.
The research extracts and screens lignin degrading bacteria from soil samples, screens and identifies degrading strains, is applied to garden waste composting, explores the influence of the garden waste composting on the composting effect, solves the environmental problem, changes the treated waste into organic fertilizer for recycling, and is a virtuous cycle process of resources.
The preservation information for the Gliocladium roseum (Clinostacchys rosea) strain YZC3 of the present invention is as follows:
naming: YZC3
And (4) classification name: gliocladium roseum
The name of Latin is: clonostachys rosea
The preservation number is as follows: CGMCC No.14782
The preservation organization: china general microbiological culture Collection center
The address of the depository institution: xilu No.1 Hospital No.3 of Beijing market facing Yang district
The preservation date is as follows: 9/2017 and 27/month
Drawings
FIG. 1 shows the results of the color reaction of guaiacol of YZC3 strain;
FIG. 2 shows the results of the aniline blue decolorization reaction of YZC3 strain;
FIG. 3 shows the experimental results of the YZC3 strain for producing LiP and MnP enzymes;
FIG. 4 shows the results of the experiment for producing Lac enzyme by YZC3 strain;
FIG. 5 is a graph of YZC3 lignin degradation rate as a function of time;
FIG. 6 is a temperature change curve of compost, wherein C3 is YZC3 group, and CK is a blank control group;
FIG. 7 is a photomicrograph of the YZC3 strain;
FIG. 8 is an ITS rDNA sequence homologation tree of strain YZC 3.
Detailed Description
The present invention is further illustrated by the following specific examples, which are intended to be illustrative only and not to limit the scope of the invention. The experimental methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are commercially available unless otherwise specified.
Source of biological material
The soil for separating the strain is collected from humus soil under the forest of the eagle peak. The dry branches and fallen leaves are sourced from the campus greening waste of Beijing college of agriculture.
Reagent and consumable
Separating a culture medium: PDA medium, beef extract peptone medium (bacteria), No.1 culture medium of gao shi (actinomycetes), chromogenic medium: PDA-guaiacol medium, decolorizing medium: PDA-aniline blue medium, all commercially available, or, according to the field of tools, according to the conventional method for preparation.
The group of embodiments provides a gliocladium roseum (Clonostachys rosea) strain YZC3 with the preservation number of CGMCC No. 14782.
Based on the content recorded in the invention, the bacterial strain can be used for preparing products for degrading lignin and products related to landscaping waste composting.
In some embodiments, the gliocladium roseum (Clonostachys rosea) strain YZC3 is formulated to degrade lignin preparations, and/or, microbial agents.
In other embodiments, the gliocladium roseum (Clonostachys rosea) strain YZC3 is formulated as a product for composting landscaping waste, and/or a bacterial agent.
Group 2 examples, articles of the invention that degrade lignin
The present set of embodiments provide an article that can degrade lignin. Characterized in that the active ingredient of the preparation comprises Gliocladium roseum (Clinostacchys rosea) strain YZC3 as described in any of example 1.
The group of embodiments provides a microbial inoculum for degrading lignin, which comprises an active ingredient for degrading lignin; characterized in that the active ingredient comprises gliocladium roseum (Clonostachys rosea) strain YZC3 as defined in any one of group 1 of the examples;
in a preferred embodiment of this group, the microbial inoculum further comprises auxiliary materials commonly used for preparing microbial inoculants.
Specifically, the auxiliary materials are selected from the culture medium of the gliocladium roseum (Clinostasys rosea) strain YZC 3; or other adjuvants commonly used in microbial agents, such as carriers, excipients, solvents, propellants, solubilizers, solubilizing agents, emulsifiers, colorants, binders, disintegrants, fillers, lubricants, wetting agents, tonicity adjusting agents, stabilizers, glidants, flavoring agents, preservatives, suspending agents, coating materials, fragrances, anti-adhesives, integration agents, penetration enhancers, pH adjusting agents, buffers, plasticizers, surfactants, foaming agents, antifoaming agents, thickening agents, encapsulation agents, humectants, absorbents, flocculating agents and deflocculating agents, filter aids, release retardants, and the like.
Group 4 articles of the invention for composting landscaping waste
The present group of embodiments provides a product for composting landscaping waste characterized in that the active ingredient of the product comprises the strain Gliocladium roseum (Clinostasys rosea) YZC3 of any of group 1 embodiments.
The group of embodiments provides a microbial inoculum for landscaping waste composting, which comprises active ingredients; characterized in that the active ingredient comprises gliocladium roseum (Clonostachys rosea) strain YZC3 as defined in any one of group 1 of the examples;
in a preferred embodiment of this group, the microbial inoculum further comprises auxiliary materials commonly used for preparing microbial inoculants.
In specific embodiments, the adjuvant is selected from the group consisting of a culture medium of the gliocladium roseum (Clonostachys rosea) strain YZC3, a carrier, an excipient, a solvent, a propellant, a solubilizer, a cosolvent, an emulsifier, a colorant, a binder, a disintegrant, a filler, a lubricant, a wetting agent, an osmotic pressure regulator, a stabilizer, a glidant, a flavoring agent, a preservative, a suspending agent, a coating material, a fragrance, an anti-adhesive, an integrating agent, an osmotic enhancer, a pH adjuster, a buffer, a plasticizer, a surfactant, a foaming agent, an antifoaming agent, a thickener, an encapsulating agent, a humectant, an absorbent, a diluent, a flocculating agent and a deflocculating agent, a filter aid, a release retardant, and the like.
EXAMPLE 6 composting method of the invention
The embodiment of the group provides a method for composting landscaping waste, which is characterized by comprising the following steps: gliocladium roseum (Clinostachys rosea) strain YZC3 according to any of example 1 was used in the composting process.
In a further embodiment, the method further comprises the conventional steps of composting landscaping waste;
in specific embodiments, the conventional steps refer to composting steps described in the landscaping waste composting technical code (DB11/T840-2011) and/or steps described in the chinese fecal sanitation standard (GB 7959-87).
In a preferred embodiment, the composting temperature is above 50 ℃ and is maintained for 5-7 days.
The present group of embodiments provides compost characterized by being prepared by adding to compost material and/or inoculating Gliocladium roseum (Clinostasys rosea) strain YZC3 as described in any of the group 1 embodiments.
In a particular embodiment of this group, the compost material comprises landscaping waste.
Experimental example, screening, identification and Effect verification of the Strain of the present invention
1 materials of the experiment
The soil for separating the strain is collected from humus soil under the forest of the eagle peak. The dry branches and fallen leaves are sourced from the campus greening waste of Beijing college of agriculture.
2 culture Medium
Separating a culture medium: PDA medium, beef extract peptone medium (bacteria), No.1 culture medium of gao shi (actinomycetes), chromogenic medium: PDA-guaiacol medium, decolorizing medium: PDA-aniline blue culture medium
3 Experimental methods
3.1 soil Strain isolation
Preparing sterile water, and mixing the soil sample according to the ratio of 10-3To 10-6The concentration gradient of (A) was dissolved in sterile water and was fully dissolved. Then the mixed solution is sucked and inoculated on a PDA culture medium, and the PDA culture medium is placed at 28 ℃ for culture for 5-7 days.
3.2 morphological identification and purification of the Strain
Primarily judging fungi, bacteria and actinomycetes according to the forms, connecting the fungi, the bacteria and the actinomycetes to respective special culture media, and repeatedly inoculating and purifying until obtaining pure strains for later use.
3.3 PDA-guaiacol Flat plate color reaction
Inoculating the purified strain on a PDA-guaiacol culture medium plate, culturing at 28 deg.C for 5d, recording whether there is red-brown color change circle and the diameter of the color change circle every day, and recording as + if there is red-brown color change circle, otherwise recording as-. And detecting the strain with higher Lac according to the time generated by the color-changing ring and the diameter of the color-changing ring.
3.4 PDA-aniline blue plate decolorizing reaction
Inoculating the purified strain on a PDA-aniline blue culture medium plate, culturing the plate at 28 ℃ in the dark for 10d, recording whether a fading circle is generated in the blue culture medium and the diameter of the fading circle, and recording as + if the fading circle is generated in the blue culture medium, otherwise recording as-. Detecting strains with high MnP and LiP yield according to the time of producing the fading circle and the diameter of the fading circle
3.5 enzyme production assay
The edges of the colonies are punched by a puncher to form holes with the diameter of 5mm, a plurality of drops of 0.1mol/L alpha-naphthol (purple color development ring-Lac) prepared by 96% ethanol are respectively dropped, and the mixed solution of 4% of the existing components and 1% of pyrogallol (yellow brown color development rings-LiP and MnP) is prepared.
3.6 enzyme Activity test
The liquid culture medium is cultured for 72 hours at 35 ℃ in a shaking table of 150r/min, 0.2ml of the liquid culture medium is inoculated into 100ml of the enzyme production culture medium, and the liquid culture medium is cultured for 48 hours under the same conditions. Sampling every 24h to determine enzyme activity, filtering the cultured liquid fermentation enzyme production culture medium with two layers of gauze, centrifuging the filtrate at 4 ℃ for 15min by a 10000r/min centrifugal machine after sampling, and taking supernatant, namely crude enzyme liquid for later use.
Method for determining activity of lignin peroxidase (LiP) -veratryl alcohol
Determination of manganese peroxide mold (MnP) Activity-Mn2+Method of
ABTS method for determining laccase (Lac enzyme) activity
3.7 Lignin degrading ability measurement
Pulverizing corn stalk, sieving with 40 mesh sieve, subpackaging in 25 250mL triangular bottles, each bottle 20g, adding 60mL nutrient solution (specifically, "seaweed microelement solution", purchased from ELITE Biotech (Shanghai) Ltd.), sterilizing at high temperature for 3h, inoculating with YZC3, and inoculating at a mass fraction of 10%. Then, the culture was carried out at an appropriate temperature (28 ℃ C.), the relative humidity in the incubator was maintained at 80%, and sampling was started after 25d and 5d of co-culture, and sampling was carried out every 5d later, and 5 flasks were taken for each treatment. Putting 2g of a sample to be detected in each bottle into a 100mL conical flask, adding 50mL of 2mol/L HCl, preserving the temperature at 100 ℃ for 50min, filtering by using a No.3 sand core funnel, and washing residues with water until the pH value is 6.5-7.0. Drying the filter residue obtained in the last step at 80 ℃, putting the filter residue together with a sand core funnel into a 150mL beaker, and adding 10mL 72% H2SO4Degrading for 4h, adding 90mL of distilled water, standing overnight at room temperature, washing the residue with distilled water the next day until the pH is 6.5, and drying until the weight is constant2，W2Incinerating in a muffle furnace at 550 ℃ to obtain ash W1The lignin content is W2-W1
The lignin degradation rate is (original corn straw lignin content-corn straw lignin content after solid state fermentation)/original corn straw lignin content is multiplied by 100 percent
3.8 compost test
Mixing collected dry branches and fallen leaves with pig manure according to a ratio of 1:1 in advance, adding bacteria and stirring uniformly, wherein each fermentation tank (the volume is 1 m)3) Adding 1L of bacterial liquid, and adding a sterilization culture medium added with YZC3 bacterial strains into the YZC3 group; CK group is blank control group, i.e. adding the same amount of sterilization culture medium as YZC3 group, and no bacteria are added in the culture medium; the procedure was repeated 3 times, and temperature measurements were taken daily during composting. And after composting for 47d, naturally airing the finally taken sample, and crushing the sample by using a crusher after airing for use when various indexes are detected. The measurement of each index comprises compost temperature, pH value, C/N, seed germination rate, conductivity, total nitrogen and total nitrogenPhosphorus, total potassium, total carbon, organic matters, organic carbon and humic acid.
TABLE 1 physicochemical Properties of compost Material
3.9 identification of the Strain
Morphological characteristic identification: inoculating the screened lignin-degrading bacteria into a PDA (personal digital assistant) plate, culturing at the constant temperature of 25 ℃ for 7d, and observing the growth condition and appearance form of bacterial colonies.
And (3) molecular identification: sequencing of fungal 18S rRNA fragment, primer sequence FR 1: AICCATTCAATCGGTAIT (SEQ ID NO.1), NS 1: GTAGTCATATGCTTGTCTC (SEQ ID NO.2), PCR amplification system 2. mu.L of DNA (70 ng/. mu.L) template; dNTPMixture (2.5mM) 2.5. mu.L; FR1 (20. mu.M) 1.5. mu.L; NS1 (20. mu.M) 1.5. mu.L; 10 XEx Taq Buffer (Mg2+ plus) 5. mu.L; ex Taq enzyme (5U/. mu.L) 0.2. mu.L; complement ddH2O to 50. mu.L. And (3) amplification procedure: denaturation at 94 deg.C for 3 min; denaturation at 94 deg.C for 1min, annealing at 50 deg.C for 1min, extension at 72 deg.C for 2min, and 30 cycles; extension at 72 ℃ for 5 min.
4 results and analysis
4.1 screening of Lignin-degrading bacteria
The soil strains are primarily separated and purified, 15 strains are separated and purified from the soil strains, the purified strains are inoculated on a PDA-guaiacol culture medium for culture and a PDA-aniline blue culture medium, as shown in figure 1 and figure 2, the results of color development and decoloration are observed, and a fungus YZC3 which can develop color and decolor is discovered.
The YZC3 strain is screened out and subjected to the next enzyme production test. The results of the LiP and MnP enzyme production experiments are shown in figures 3 and 4, the mixed solution of 4 percent and 1 percent of pyrogallol which are prepared in situ is dripped into the hole punched in the flat plate, and YZC3 shows a tawny color circle, which proves that the strain can produce LiP and MnP enzyme. The Lac enzyme production experiment is that 0.1mol/L alpha-naphthol solution prepared by 96% ethanol is dripped into a perforated hole in a flat plate, and YZC3 shows a purple color ring, which proves that the fungus can produce Lac.
The activity of YZC3 lignin lyase is tested, and the YZC3 is found to have strong LiP production capability and has a peak value at 7 days, and the activity is 12.903U/L. The YZC3 has a slightly strong MnP generating capacity, and the peak value is 104U/L, and the enzyme activity peak appears at 10-12 d. The Lac produced by YZC3 is the activity peak at 8d, and is 5.556U/L.
As is clear from the data in FIG. 5, the results of the measurement of the lignin-degrading ability of YZC3 show that the lignin-degrading rate of YZC3 increased with the lapse of the culture time of the solid state fermentation, and that the lignin-degrading rate reached a maximum of 79.2% at 25 d.
4.2 Effect of Lignin degrading bacteria YZC3 on composting of landscaping waste
4.2.1 YZC3 Effect on compost maturity
The temperature is an important index for judging the composting degree, generally, the temperature in the composting process is subjected to 4 periods of obvious temperature rising period, high temperature period, temperature lowering period and after-ripening period, and the compost is considered to be primarily mature when the temperature of the compost returns to the ambient temperature again. From FIG. 6, it can be seen that the temperature rapidly increased to the maximum temperature at the initial stage of composting, decreased to below 50 ℃ after a period of 6-7 days, and returned to ambient temperature after a longer period of after-ripening. According to the innocent sanitary standard of the excrement in China (GB7959-87), the composting temperature is more than 50 ℃ and is maintained for 5-7 days, so that the innocent sanitary standard of the excrement can be met. The high-temperature continuous days of the compost all exceed 5 days, and the high-temperature days of the YZC3 group are 1 day more than that of the CK group, which shows that the YZC3 is beneficial to prolonging the high-temperature time of the compost.
The germination index of the seeds can be used for judging the phytotoxicity of the compost and is one of important parameters of the index of the maturity. It is widely accepted by many researchers that when the germination index GI reaches 80% -85%, the compost is considered to be non-phytotoxic or composted. It can be seen from table 2 that at 47 days of composting, the germination index at each treatment was greater than 80%, and there was no significant difference, indicating that at 47 days of composting, each treated compost was well-decomposed.
The carbon-nitrogen ratio is an important reference index of the compost maturity, the lower the carbon-nitrogen ratio indicates the higher the compost maturity, and the carbon-nitrogen ratio is generally considered to reach 20-30 compost basic maturity, as shown in table 2, the difference between treatments is remarkable, which indicates that the YZC3 is helpful for the compost maturity and reduces the carbon-nitrogen ratio.
TABLE 2 microbial inoculum compost maturity index
In the above table, the column of "secondary fermentation" refers to the number of days during which secondary temperature rise occurs during composting; conductivity is another indicator of the degree of decomposition, and is the ability of a solution to conduct current, expressed numerically, with higher ion concentrations being more conductive. The higher the ion concentration in the fertilizer, the more thorough the process of inorganic salt ions formed by degrading organic matters in the compost material is, and the higher the compost maturity is. The conductivity of the YZC3 treatment is significantly higher than that of the blank, and the fact that the YZC3 has a positive effect on the decomposition degree of the compost and contributes to the complete decomposition of the compost is also demonstrated.
4.2.2 YZC3 Effect on compost quality
Humus is an important organic carbon library, which contains a large number of functional groups (such as carboxyl, phenolic hydroxyl and the like) and can adsorb and fix heavy metal ions, so that the generation of the humus has an important influence on the quality of compost. The degradation of lignin plays an important role in the formation of humic acid. Under the action of microorganisms, the side chain of lignin is oxidized into lignin derivatives, which form the core skeleton of humus. Table 2 shows that the content of humic acid in YZC3 compost products is significantly higher than the blank. YZC3 is beneficial to accelerating lignin degradation and forming humic acid.
The total effective nutrient and the available nutrient are important indexes for evaluating the fertilizer, and the higher the nutrient content is, the better the compost quality is. YZC3 has a significant effect on TN content in the compost, helping to reduce N loss during composting.
TABLE 2 nutrient index of microbial inoculum compost
4.3 identification of the Strain
YZC3 grows faster on a potato glucose agar culture medium, is cultured for 7 days at 25 ℃ in the dark, and has the colony diameter of 40-43 mm, is flocculent and white, and has light yellow middle part; the back of the colony is light yellow, and the pigment does not permeate into the culture medium. Conidiophores are tall, 200 mu m long, 3.0-5.0 mu m wide, broom-shaped or wheel-shaped branches, spore-forming cell flask-shaped (ITS rDNA broom-shaped branches) or lanceolate-shaped (wheel-shaped branches), 9.0-38.5 multiplied by 1.5-2.5 mu m wide and less than 1 mu m wide at the top end; conidia are oval, kidney-shaped, colorless, smooth in wall, 3.3-6.7 multiplied by 2.0-3.2 microns, and are aggregated into clusters, and the microscopic morphology of the strain is shown in figure 7.
The sequencing result of the 18S rRNA fragment of the fungus is shown in SEQ ID NO.3, the homology of the YZC3 strain and gliocladium roseum (Clinostasys rosea) is the highest and reaches 100 percent by comparison, and the homology comparison phylogenetic tree is shown in FIG. 8.
SEQUENCE LISTING
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Claims (12)
1. A Gliocladium roseum (Clinostacchys rosea) strain YZC3 with preservation number of CGMCC No. 14782.
2. A lignin-degradable article characterized in that the active ingredient of said article comprises the strain gliocladium roseum (Clonostachys rosea) YZC3 of claim 1.
3. A microbial agent for degrading lignin, comprising an active ingredient for degrading lignin; characterized in that the active ingredient comprises the gliocladium roseum (Clonostachys rosea) strain YZC3 of claim 1.
4. A microbial inoculum for degrading lignin according to claim 3, comprising an active ingredient for degrading lignin; the method is characterized in that the microbial inoculum also comprises auxiliary materials commonly used for preparing the microbial inoculum.
5. A product for composting landscaping waste characterized in that the active ingredient of the product comprises the scopularia rosea (Clonostachys rosea) strain YZC3 of claim 1.
6. The microbial inoculum for the garden greening waste compost comprises active ingredients; characterized in that the active ingredient comprises the gliocladium roseum (Clonostachys rosea) strain YZC3 of claim 1.
7. The microbial inoculum for composting the landscaping waste of claim 6, further comprising adjuvants commonly used for preparing microbial inoculum.
8. A method for composting landscaping waste, comprising: use of the gliocladium roseum (Clonostachys rosea) strain YZC3 of claim 1 in composting processes.
9. The method of claim 8, further comprising the conventional step of composting landscaping waste.
10. The method according to claim 8 or 9, characterized in that the composting temperature is above 50 ℃ and is maintained for 5-7 days.
11. Compost, characterized in that it is prepared by adding or inoculating Gliocladium roseum (Clinostachys rosea) strain YZC3 according to claim 1 to compost material.
12. A compost as claimed in claim 11, wherein said compost material comprises landscaping waste.
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