CN110042063A - A kind of rouge and powder glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom and its application - Google Patents
A kind of rouge and powder glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom and its application Download PDFInfo
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- CN110042063A CN110042063A CN201910329776.0A CN201910329776A CN110042063A CN 110042063 A CN110042063 A CN 110042063A CN 201910329776 A CN201910329776 A CN 201910329776A CN 110042063 A CN110042063 A CN 110042063A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
Abstract
The present invention " a kind of rouge and powder glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom and its application ", belongs to microorganisms technical field.The deposit number of the rouge and powder gliocladium germ strain YZC3 is CGMCC No.14782.Based on rouge and powder gliocladium germ strain YZC3, the present invention also provides a kind of product of degradable lignin, the microbial inoculum for lignin degrading, the product for afforestation castoff compost, the microbial inoculum for afforestation castoff compost and a kind of methods of afforestation castoff compost.Rouge and powder gliocladium germ strain YZC3 of the invention has the effect of excellent in terms of lignin degrading.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to a kind of viscous mould (Clonostachys rosea) bacterium of broom of rouge and powder
Strain YZC3 and its application.
Background technique
Biomass energy is highly important renewable energy, and important component of the lignin as biomass is standby always
It is concerned.Since 20th century, domestic and international some experts and scholars begin to explore the optimal path of lignin degrading.It is involved
Research method mainly have physical method, chemical method, bioanalysis etc..Wherein biological degradation method is because it has action condition mild, single-minded
The advantages that property is strong, environmental and cost is relatively low and be concerned.Lignin is the biological high score being widely present in biomass
Son, monomer structure are phenyl-propane.Lignin in annual garden waste can be with crop straw burning or pulp-making waste-water discharge etc.
Mode is directly discarded, or directly discarded to accumulate, and causes the waste of biomass resource.In the recycle to lignin
In, biodegrade can make lignin conversion small-molecule substance, and then isolate and purify as industrial chemicals.Its with it is low in cost,
The advantages such as secondary pollution is small, are more favored compared to physics and chemical method.Lignin structure is complicated and stablizes, and biodegrade is difficult, therefore
And the degradation capability of microorganism is deciding factor.
Practice have shown that microbial degradation lignin is optimal approach, this method has degradation rate height, safety and environmental protection, cost
The advantages that low, renewable.Meanwhile exist in nature largely can be with the microorganism of lignin degrading, including fungi, bacterium, puts
Line bacterium is mainly made of the micropopulation from soil and animal alimentary canal.
For example, patent application 201711207383.X provides a kind of myrothecium verrucaria (Myrothecium
Verrucaria) mutant strain T2901, deposit number are CCTCC NO:M2017413, and the bacterial strain Lignin degradation rate is reachable
42.30%.
What patent application 201711202906.1 disclosed is a kind of composite bacteria agent of degrading maize straws, and active constituent is
Aspergillus niger (Aspergillus), trichoderma (Trichoderma), penicillium oxalicum (Penicllium), Phanerochaete chrysosporium
(Phanerochaete chrysosporium)；Or aspergillus niger (Aspergillus) fermentation liquid, trichoderma (Trichoderma) hair
Zymotic fluid, penicillium oxalicum (Penicllium) fermentation liquid, Huang archespore Mao Pingge (Phanerochaete chrysosporium) hair
Zymotic fluid, the composite bacteria agent Lignin degradation rate are 28.5%.
This field it is still necessary to screen, develop more better new strains of lignin degradation effect, for production practices selection and
It uses.
Summary of the invention
The demand based on this field, the present invention extracted from soil sample filter out one plant of rouge and powder glue broom it is mould
(Clonostachys rosea) bacterial strain YZC3, identified, which can effective lignin degrading and to can be applied to gardens discarded
Object compost.
Technical scheme is as follows:
A kind of rouge and powder viscous broom mould (Clonostachys rosea) bacterial strain YZC3, deposit number CGMCC
No.14782。
A kind of product of degradable lignin, which is characterized in that the active constituent of the product includes described in claim 1
Rouge and powder glue mould (Clonostachys rosea) the bacterial strain YZC3 of broom.
A kind of microbial inoculum for lignin degrading, including the active constituent for lignin degrading；It is characterized in that, described
Active constituent includes that rouge and powder described in claim 1 glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom；
Preferably, the microbial inoculum further includes preparing the common auxiliary material of microbial inoculum.
Product for afforestation castoff compost, which is characterized in that the active constituent of the product includes that right is wanted
Rouge and powder described in asking 1 glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom.
For the microbial inoculum of afforestation castoff compost, including active constituent；It is characterized in that, the active constituent includes
Rouge and powder described in claim 1 glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom；
Preferably, the microbial inoculum further includes preparing the common auxiliary material of microbial inoculum.
A kind of method of afforestation castoff compost characterized by comprising claim is used in composting process
Rouge and powder described in 1 glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom.
The method also includes the conventional steps of afforestation castoff compost；
Preferably, compost temperature at 50 DEG C or more and maintains 5~7d.
A kind of compost, which is characterized in that by being added in compost material, and/or, being inoculated with the rouge and powder, to glue broom mould
(Clonostachys rosea) bacterial strain YZC3 is prepared.
The compost material includes afforestation waste.
This research just extracts screening lignin-degrading bacteria from soil sample and carries out screening and identification to degradation bacteria strains, and is applied to
Garden waste compost explores its influence to compost effect, both solves environmental problem, and the waste after having handled becomes again
It is the process of resource benign cycle for organic fertilizer recycling.
The preservation information that rouge and powder of the invention glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom is as follows:
Name: YZC3
Specific name: it is mould that rouge and powder glues broom
Latin name: Clonostachys rosea
Deposit number: CGMCC No.14782
Preservation mechanism: China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation organization address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3
Preservation date: on September 27th, 2017
Detailed description of the invention
Fig. 1 is YZC3 bacterial strain guaiacol chromogenic reaction result；
Fig. 2 is YZC3 bacterial strain aniline blue decoloring reaction result；
Fig. 3 is that YZC3 bacterial strain produces LiP, MnP enzyme experimental result；
Fig. 4 is that YZC3 bacterial strain produces Lac enzyme experimental result；
Fig. 5 is that YZC3 Lignin degradation rate changes over time；
Fig. 6 is compost temperature change curve, and the C3 in figure is YZC3 group, and CK is blank control group；
Fig. 7 is the microscopic morphology figure of YZC3 bacterial strain；
Fig. 8 is YZC3 bacterial strain ITS rDNA sequence homology chadogram.
Specific embodiment
The present invention is described in further detail combined with specific embodiments below, and following embodiments are merely exemplary,
It is not intended to limit the scope of the present invention.Experimental method used in following embodiments is conventional side unless otherwise specified
Method；Material, reagent used etc., are commercially available unless otherwise specified.
Biological material source
The soil collection of strain is separated from fertile soil under vulture peak forest.Dry branches and fallen leaves is useless from Beijing Agricultural College's afforestation design
Object.
Reagent and consumptive material
Isolation medium: PDA culture medium, beef-protein medium (bacterium), No. 1 culture medium (actinomyces) of Gao Shi,
Chromogenic culture medium: PDA- guaiacol culture medium, decoloration culture medium: PDA- aniline blue culture medium, it is commercially available, or, root
It is prepared according to the conventional method that this field reference book is recorded.
1st group of embodiment, bacterial strain of the invention
This group of embodiment provides a kind of viscous mould (Clonostachys rosea) bacterial strain YZC3 of broom of rouge and powder, and deposit number is
CGMCC No.14782。
Based on the content that the present invention records, bacterial strain of the invention can be used to prepare product and the gardens of lignin degrading
Afforest the relevant product of castoff compost.
In some embodiments, viscous mould (Clonostachys rosea) the bacterial strain YZC3 of broom of the rouge and powder is made into degradable
The product of lignin, and/or, microbial inoculum.
In further embodiments, the rouge and powder glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom and is made for
The product of afforestation castoff compost, and/or, microbial inoculum.
The product of 2nd group of embodiment, the degradable lignin of the present invention
This group of embodiment provides a kind of product of degradable lignin.It is characterized in that, the active constituent packet of the product
It includes the 1st group of described in any item rouge and powder of embodiment and glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom.
The microbial inoculum of 3rd group of embodiment, the degradable lignin of the present invention
This group of embodiment provides a kind of microbial inoculum for lignin degrading, including the active constituent for lignin degrading；
It is characterized in that, the active constituent includes that the 1st group of described in any item rouge and powder of embodiment glue the mould (Clonostachys of broom
Rosea) bacterial strain YZC3；
In this group of preferred embodiment, the microbial inoculum further includes preparing the common auxiliary material of microbial inoculum.
Specifically, the auxiliary material is selected from, and the rouge and powder glues the training of mould (Clonostachys rosea) the bacterial strain YZC3 of broom
Support base；Or other microbial inoculums often use auxiliary material, for example, carrier, excipient, solvent, propellant, solubilizer, cosolvent, emulsifier,
Toner, disintegrating agent, filler, lubricant, wetting agent, osmotic pressure regulator, stabilizer, glidant, corrigent, is prevented binder
Rotten agent, suspending agent, coating material, aromatic, anti-binder, integrated agent, penetration enhancer, pH adjusting agent, buffer, increasing
Mould agent, surfactant, foaming agent, defoaming agent, thickener, inclusion agents, moisturizer, absorbent, diluent, flocculant and anti-
Flocculant, filter aid, release retarding agent etc..
4th group of embodiment, the present invention are used for the product of afforestation castoff compost
This group of embodiment provides a kind of product for afforestation castoff compost, which is characterized in that the product
Active constituent includes that the 1st group of described in any item rouge and powder of embodiment glue mould (Clonostachys rosea) the bacterial strain YZC3 of broom.
5th group of embodiment, the present invention are used for the microbial inoculum of afforestation castoff compost
This group of embodiment provides a kind of microbial inoculum for afforestation castoff compost, including active constituent；Its feature exists
In the active constituent includes that the 1st group of described in any item rouge and powder of embodiment glue mould (Clonostachys rosea) bacterial strain of broom
YZC3；
In this group of preferred embodiment, the microbial inoculum further includes preparing the common auxiliary material of microbial inoculum.
In the particular embodiment, the auxiliary material is selected from, and the rouge and powder glues mould (Clonostachys rosea) bacterium of broom
The culture medium of strain YZC3, excipient, solvent, propellant, solubilizer, cosolvent, emulsifier, colorant, binder, collapses carrier
Solve agent, filler, lubricant, wetting agent, osmotic pressure regulator, stabilizer, glidant, corrigent, preservative, suspending agent, packet
Clothing material, aromatic, anti-binder, integrated agent, penetration enhancer, pH adjusting agent, buffer, plasticizer, surface-active
Agent, foaming agent, defoaming agent, thickener, inclusion agents, moisturizer, absorbent, diluent, flocculant and deflocculant, filter aid,
Discharge retarding agent etc..
6th group of embodiment, compost method of the invention
This group of embodiment provides a kind of method of afforestation castoff compost characterized by comprising in composting process
It is middle to glue mould (Clonostachys rosea) the bacterial strain YZC3 of broom using the 1st group of described in any item rouge and powder of embodiment.
In a further embodiment, the method also includes the conventional steps of afforestation castoff compost；
In the particular embodiment, the conventional steps refer to, " afforestation castoff compost technical regulation (DB11/
T840-2011 the composting step recorded in) ", and/or, it is recorded in " Chinese decontamination of human excreta sanitary standard (GB7959-87) "
The step of.
In a preferred embodiment, compost temperature at 50 DEG C or more and maintains 5~7d.
7th group of embodiment, compost of the invention
This group of embodiment provides a kind of compost, which is characterized in that by being added in compost material, and/or, be inoculated with the 1st group it is real
Viscous mould (Clonostachys rosea) the bacterial strain YZC3 of broom of the described in any item rouge and powder of example is applied to be prepared.
In this group of specific embodiment, the compost material includes afforestation waste.
Experimental example, the screening of bacterial strain of the present invention, identification and compliance test result
1 experimental material
The soil collection of strain is separated from fertile soil under vulture peak forest.Dry branches and fallen leaves is useless from Beijing Agricultural College's afforestation design
Object.
2 culture mediums
Isolation medium: PDA culture medium, beef-protein medium (bacterium), No. 1 culture medium (actinomyces) of Gao Shi,
Chromogenic culture medium: PDA- guaiacol culture medium, decoloration culture medium: PDA- aniline blue culture medium
3 experimental methods
3.1 soil strain separatings
Sterile water is prepared, soil sample is pressed 10-3To 10-6Concentration gradient be dissolved into sterile water, sufficiently dissolve.Then it inhales
It takes mixed liquor to be seeded in PDA culture medium, is placed in 5~7d of culture at 28 DEG C.
3.2 strain Morphological Identifications and purifying
It according to form preliminary judgement fungi, bacterium and actinomyces, and is connected on respective special culture media, repeatedly inoculation purifying
Until pure bacterial strain is obtained, it is spare.
3.3 PDA- guaiacol plate chromogenic reactions
It after strain after purification is connected on PDA- guaiacol culture medium flat plate, sets and cultivates 5d at 28 DEG C, record daily
Whether there is or not rufous discoloration circle generation and change colour loop diameter size, have reddish brown chromosphere person be denoted as+, otherwise be denoted as-.According to change
The higher bacterial strain of Lac is produced in the diameter of time caused by chromosphere and discoloration circle, detection.
3.4 PDA- aniline blue plate decoloring reactions
Strain after purification is connected on PDA- aniline blue culture medium flat plate, and plate is set and is protected from light culture 10d at 28 DEG C,
Record in blue culture medium whether there is or not the generation for the circle that fades and the size for the loop diameter that fades, have circle person of fading be denoted as+, otherwise note
For-.According to the time of circle generation and the diameter for the circle that fades of fading, detects and produce the higher bacterial strain of MnP and LiP
The test of 3.5 producing enzymes
Colony edge beats the hole diameter 5mm with punch, is respectively dropped into α-naphthalene that few drops of 0.1mol/L are prepared with 96% ethyl alcohol
Phenol (purple colour developing circle-Lac), the pyrogaelol of equivalent 4% and 1% mixed liquor now matched (yellowish-brown colour developing circle-LiP, MnP).
The test of 3.6 enzyme activity
Liquid Culture is based on 35 DEG C, cultivates 72h in 150r/min shaking table, 0.2ml is taken to be inoculated in 100ml culture medium
In, 48h is cultivated under the same terms.Every enzyme activity is measured by sampling for 24 hours, cultured liquid fermentation culture medium is first used two
Layer filtered through gauze, after the completion of sampling, then by filtrate in 4 DEG C, 10000r/min centrifuge is centrifuged 15min, takes supernatant, i.e., slightly
Enzyme solution processed, for use.
Lignin peroxidase (LiP) active measurement --- veratryl alcohol method
Mould (MnP) the active measurement of manganese peroxidase --- Mn2+Method
Laccase (Lac enzyme) active measurement --- ABTS method
The measurement of 3.7 lignin degradation abilities
Corn straw smashing is crossed into 40 meshes, is sub-packed in 25 250mL triangular flasks, every bottle of 20g adds people's 60mL nutrient solution
(specially " Trace Elements In Seaweeds solution " is purchased from ELITE biotech (Shanghai) Ltd company), high-temperature sterilization 3h connects
Enter YZC3 inoculation, mass fraction 10%.Then thermophilic culture (28 DEG C), relative humidity is maintained at 80% in incubator, trains altogether
25d is supported, starts to sample after 5d, later every 5d takes a sample, and each processing takes 5 bottles.Every bottle takes 2g sample to be tested to be placed in 100mL
In conical flask, adds people 50mL2mol/L HCl, filtered after 100 DEG C of heat preservation 50min with No. 3 sand core funnels, rinsing residue to pH is
6.5~7.0.80 DEG C of filter residue obtained in the previous step are dried, is placed in 150mL beaker together with sand core funnel, adds people 10mL72%
H2SO4, degrade 4h, adds people's 90mL distilled water, ambient temperature overnight, secondary daily distilled water rinsing residue is dried to perseverance to pH=6.5
Weight is W2, W2It is ashed in 550 degree of Muffle furnace, obtains ash content W1, content of lignin=W2-W1
Lignin degradation rate=(Spruce lignin content after original Spruce lignin content-solid state fermentation)/
Original Spruce lignin content × 100%
The experiment of 3.8 compost
The dry branches and fallen leaves gathered is mixed with pig manure by 1:1 in advance, while bacterium is added and stirs evenly, Mei Gefa
Ferment pond (volume 1m3) adding 1L bacterium solution, the sterilising medium for being added to YZC3 bacterial strain is added in YZC3 group；CK group is blank control
Group is added the sterilising medium with YZC3 group equivalent, bacterium is not added in culture medium；It is repeated 3 times, carries out temperature during compost daily
Degree measurement.After compost 47d, the sample finally taken is subjected to naturally dry, when being crushed with pulverizer in case surveying various indexs after drying
It uses.Measure indices measurement include compost temperature, pH value, C/N, percentage of seedgermination, conductivity, full nitrogen, full phosphorus, full potassium,
Full carbon, organic matter, organic carbon, humic acid.
1 composting material physicochemical property table of table
The identification of 3.9 bacterial strains
Morphological feature identification: the lignin-degrading bacteria filtered out being inoculated in PDA plate, 25 DEG C, constant temperature incubation 7d,
Observe growing state, the mode of appearance of its bacterium colony.
Molecular Identification: fungi 18S rRNA sequencing fragment, primer sequence FR1:AICCATTCAATCGGTAIT (SEQ ID
NO.1), NS1:GTAGTCATATGCTTGTCTC (SEQ ID NO.2), PCR amplification system: 2 μ L of DNA (70ng/ μ L) template；
dNTPMixture(2.5mM)2.5μL；FR1(20μM)1.5μL；NS1(20μM)1.5μL；10×Ex Taq Buffer(Mg2+
plus)5μL；Ex Taq enzyme (5U/ μ L) 0.2 μ L；Supply ddH2O to 50 μ L.Amplification program: 94 DEG C of denaturation 3min；94 DEG C of denaturation
1min, 50 DEG C of annealing 1min, 72 DEG C of extension 2min, 30 recycle；72 DEG C of extension 5min.
4 results and analysis
The screening of 4.1 lignin-degrading bacterias
The isolation and purification preliminary to soil strain, therefrom isolates and purifies 15 kinds, and strain after purification is connected to PDA- and is cured
It creates on the wooden phenol culture medium in culture and PDA- aniline blue culture medium, as depicted in figs. 1 and 2, observation colour developing, decoloration are as a result, discovery
A kind of fungi YZC3 that can also decolourize that can develop the color.
To YZC3 bacterium is filtered out, the test of next step producing enzyme is carried out.LiP, MnP producing enzyme experimental result are as shown in Figure 3 and Figure 4,
The pyrogaelol mixed liquor of equivalent 4% and 1% now matched is added dropwise at punching into plate, yellowish-brown colour developing circle occurs in YZC3, it was demonstrated that the bacterium
LiP, MnP enzyme can be generated.The experiment of Lac producing enzyme is α-naphthalene that dropwise addition 0.1mol/L is prepared with 96% ethyl alcohol at punching into plate
There is purple colour developing circle in phenol solution, YZC3, it was demonstrated that this fungi can generate Lac.
YZC3 lignin decomposition enzyme vigor is tested, discovery YZC3 had stronger production LiP ability, and at the 7th day
There is peak value, vigor 12.903U/L.YZC3 production MnP ability is slightly strong, enzyme activity peak occurs in 10-12d, peak value is
104U/L.It is vigor peak value occur in 8d that YZC3, which produces Lac, is 5.556U/L.
To the measurement result of YZC3 lignin degradation ability by the data of Fig. 5 it is found that YZC3 to the degradation rate of lignin with
The extension of solid state fermentation incubation time and increase, when 25d, has reached maximum value 79.2% to the degradation rate of lignin.
Influence of the 4.2 lignin-degrading bacteria YZC3 to afforestation castoff compost
4.2.1 influence of the YZC3 to compost maturity
Temperature is to judge the important indicator of compost maturity degree, and temperature undergoes apparent liter in general in composting process
Wen Qi, the megathermal period, 4 periods of cooldown period and latter stage of ripening, when compost temperature come back to environment temperature can be considered compost tentatively at
It is ripe.Compost initial stage is quickly ramped up to maximum temperature as seen from Figure 6, and temperature drops to 50 DEG C hereinafter, after longer after continuing 6-7d
After the ripe phase, temperature revert to environment temperature.Chinese decontamination of human excreta sanitary standard (GB7959-87) regulation, compost temperature is 50
DEG C or more and maintain 5~7d, can reach and meet decontamination of human excreta sanitary standard.This compost respectively handles high temperature continuous days
More than 5d, YZC3 group is handled high temperature number of days ratio CK group more 1 days, illustrates that YZC3 is conducive to the extension of compost high-temperature time.
Seed germination index can be used to judge the phytotoxicity of compost, be one of important parameter of maturity indexes.It is many
More researchers it is believed that when germination index GI reaches 80%-85%, compost be just believed that no phytotoxicity or
Say that compost is decomposed.As seen from Table 2 at compost 47 days, germination index is managed everywhere and is all larger than 80%, and without marked difference, is said
It is bright at compost 47 days, it is each that handle compost decomposed.
Carbon-nitrogen ratio is an important reference indicator of compost maturity degree, and carbon-nitrogen ratio is lower to show that decomposed degree is higher, one
As think, when carbon-nitrogen ratio reaches, 20-30 compost is substantially decomposed, and as shown in table 2, otherness is significant between processing, illustrates that YZC3 has
Help the decomposed of compost, reduces carbon-nitrogen ratio.
2 microbial inoculum compost maturity level index of table
In upper table, " secondary fermentation " one arranges the number of days for referring to and secondary temperature elevation occurring in composting process；Conductivity is also that evaluation is rotten
Another index of ripe degree, conductivity are the ability of solution conduction electric current numerically, the more highly conductive ability of ion concentration
It is stronger.Fertilizer intermediate ion concentration is higher, shows that the process for the inorganic ion that organic matter degradation is formed in composting material is more thorough
Bottom illustrates that compost maturity degree is higher.YZC3 processing conductivity is significantly higher than blank, also illustrates YZC3 to the decomposed journey of compost
Degree has positive influence, facilitates the complete decomposed of compost.
4.2.2 influence of the YZC3 to compost quality
Humus is important organic carbon pool, wherein contain a large amount of functional group (such as carboxyl, phenolic hydroxyl group), can adsorb with
Fixed heavy metal ion, therefore, the generation of humus has compost quality important influence.The degradation of lignin is to humic
The formation of acid plays an important role.For lignin under the action of microorganism, side chain is oxidized to lignan derivatives, constitutes
The core skeleton of humus.The visible YZC3 composting production content of humic acid of table 2 is significantly higher than blank.YZC3 is conducive to accelerate
Lignin degradation forms humic acid.
Full effect nutrient, available nutrient is the important indicator for evaluating fertilizer, and nutrient content is higher, and compost quality is better.YZC3
TN content in compost is had a significant impact, the loss for reducing N in composting process is facilitated.
2 microbial inoculum compost nutrient of table
4.3 strain idenfication results
YZC3 is grown comparatively fast on potato dextrose agar, is cultivated 7 days under 25 DEG C of dark conditions, colony diameter
40~43mm, cotton-shaped, white, middle part are slightly light yellow；The bacterium colony back side is light yellow, and pigment does not penetrate into culture medium.Conidiophore
It is tall and big, 200 μm long, 3.0~5.0 μm wide, broom shape or colyliform branch, conidiogenous cell flask shape (ITS rDNA broom shape branch) or
Lanceolar (colyliform branch), 9.0~38.5 × 1.5~2.5 μm, top is wide less than 1 μm；Conidium ellipse, kidney shape, nothing
Color, wall is smooth, and 3.3~6.7 × 2.0~3.2 μm, aggregation is agglomerating, and the microscopic morphology of bacterial strain is detailed in Fig. 7.
Fungi 18S rRNA sequencing fragment result glues broom as shown in SEQ ID NO.3, by comparing YZC3 bacterial strain and rouge and powder
Mould (Clonostachys rosea) homology highest reaches 100%, and homologous comparison chadogram is as shown in Figure 8.
SEQUENCE LISTING
<110>Beijing Agricultural College
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gggctttggc cgatccccaa caccacgccc taggggcatg agggttgaaa tgacgctcag 180
acaggcatgc ccgccagaat actggcgggc gcaatgtgcg ttcaaagatt cgatgattca 240
ctgaattctg caattcacat tacttatcgc atttcgctgc gttcttcatc gatgccagaa 300
ccaagagatc cgttgttgaa agtttttatt tatttgtaaa aactactcag aagattccaa 360
aataaaacaa gaattaagtt tcctaggcgg gcgcctgatc cggggcacac gaggcgcccg 420
gggcaatccc gccgaagcaa cagtaggtat gttcacatgg gtttgggagt tgtaaactcg 480
gtaatgatcc ctccgctggt tcaccaacgg agaccttgtt acga 524
Claims (9)
1. a kind of rouge and powder glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom, deposit number is CGMCC No.14782.
2. a kind of product of degradable lignin, which is characterized in that the active constituent of the product includes described in claim 1
Rouge and powder glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom.
3. a kind of microbial inoculum for lignin degrading, including the active constituent for lignin degrading；It is characterized in that, the work
Property ingredient include rouge and powder described in claim 1 glue mould (Clonostachys rosea) the bacterial strain YZC3 of broom；
Preferably, the microbial inoculum further includes preparing the common auxiliary material of microbial inoculum.
4. being used for the product of afforestation castoff compost, which is characterized in that the active constituent of the product includes claim 1
The rouge and powder glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom.
5. being used for the microbial inoculum of afforestation castoff compost, including active constituent；It is characterized in that, the active constituent includes power
Benefit require 1 described in rouge and powder glue mould (Clonostachys rosea) the bacterial strain YZC3 of broom；
Preferably, the microbial inoculum further includes preparing the common auxiliary material of microbial inoculum.
6. a kind of method of afforestation castoff compost characterized by comprising use claim 1 in composting process
The rouge and powder glues mould (Clonostachys rosea) the bacterial strain YZC3 of broom.
7. according to the method described in claim 6, it is characterized in that, further including the conventional steps of afforestation castoff compost；
Preferably, compost temperature at 50 DEG C or more and maintains 5~7d.
8. a kind of compost, which is characterized in that by being added in compost material, and/or, it is inoculated with rouge and powder described in claim 1 and glues broom
Mould (Clonostachys rosea) bacterial strain YZC3 is prepared.
9. compost according to claim 8, which is characterized in that the compost material includes afforestation waste.
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Cited By (1)
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Effective date of registration: 20220614 Address after: 102109 No.51, Dongguan District 2, dabelao village, Jiuxian Town, Yanqing District, Beijing Patentee after: BEIJING DONGXIANG ENVIRONMENTAL TECHNOLOGY Co.,Ltd. Address before: 102206 No. 7 Nong Road, Huilongguan, Beijing, Changping District Patentee before: BEIJING University OF AGRICULTURE |