CN103898029A - Waste garden branch and leaf fermenting bacterial preparation as well as preparation method and application thereof - Google Patents
Waste garden branch and leaf fermenting bacterial preparation as well as preparation method and application thereof Download PDFInfo
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- CN103898029A CN103898029A CN201410159682.0A CN201410159682A CN103898029A CN 103898029 A CN103898029 A CN 103898029A CN 201410159682 A CN201410159682 A CN 201410159682A CN 103898029 A CN103898029 A CN 103898029A
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- useless branches
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention belongs to the technical field of microbial engineering, and in particular relates to a waste garden branch and leaf fermenting bacterial preparation as well as a preparation method and an application thereof. The waste garden branch and leaf fermenting bacterial preparation mainly comprises the following microbial components in parts by weight: 0.1-0.3 part of aspergillus, 0.1-0.3 part of penicillium, 0.2-0.4 part of trichoderma and 0.2-0.5 part of coprinus sterquilinus. The waste garden branch and leaf fermenting bacterial preparation is used for treating waste garden branches and leaves, and the wastes can be automatically fermented without inoculating other strains, so that the biotransformation rate is high, stench is not produced, and pests such as mosquitoes and flies are not bred.
Description
One, technical field
The invention belongs to microbial engineering field, be specifically related to useless branches and leaves fermenting agent in a kind of gardens and its preparation method and application.
Two, background technology
Giving up in gardens, branches and leaves (Gardenwaste) refer to that ornamental plant withers and falls naturally or manually prune deadwood, fallen leaves, residual flower, trees and the shrub beta pruning and other plant residue etc. that produce, and composition is take organic matter as main, and Fiber and lignin concentration is higher.Along with the fast development of urban afforestation in the each big city of China, the useless branches and leaves in gardens are as also increasing in the amount of dry branches and fallen leaves (Litter), branch pruning thing, turf-mown thing and residual flower etc.For a long time, neat and artistic in order to pursue, the useless branches and leaves of this city trees and shrubs are often regarded as municipal solid wastes, become the removing object of greenery patches maintenance management, and the cleaning degree of dry branches and fallen leaves even becomes the important indicator of weighing maintenance fine level.The conventional process mode of the useless branches and leaves in gardens is mainly landfill or burning.Landfill easy handling, but the material resources of conventionally employing a tremendous amount of manpower take again valuable urban land resource, and along with the rise of Urban Land Price, landfill cost is more and more higher; Burning can reduce rapidly volume of waste, and processing speed is fast, produces heat energy and can be used as heat energy power-generating, but often cause atmosphere polluting problem because of the improper of burning condition and means, and then reduce city and outskirts of a town environmental quality.Processing the useless branches and leaves of city trees and shrubs by composting process not only can turn waste into wealth, can also save the shared soil of garbage loading embeading, and alleviating the pollution of waste incineration to atmosphere, it is obviously better than additive method and becomes the Waste Disposal Technology that has development potentiality at present most in safety and economic aspect.But traditional composting mode cycle is long, efficiency is low, in fermenting process, the release of the gas such as volatilization and hydrogen sulfide of ammonia can cause serious environmental pollution, also has the shortcomings such as finished product fertilizer efficiency is low simultaneously.Process rationally and effectively the useless branches and leaves in gardens and become a science urgently to be resolved hurrily and problem of industry.
In the useless branches and leaves in gardens, exceeding 60% for Mierocrystalline cellulose and hemicellulose, is very good biomass resource, by being widely used in the aspects such as feed, fertilizer, biogas after bio-transformation.But the natural structure of the useless branches and leaves in gardens is very complicated, biotransformation efficiency is lower, and it is directly larger as the difficulty of above related products exploitation, and cost is higher.Therefore, it is an of paramount importance link in the useless branches and leaves recycling in gardens that the useless branches and leaves in gardens are carried out to effective pre-treatment, namely by the destruction to the peripheral matrix of the useless branches and leaves in gardens, as: to the composite structure depolymerization of hemicellulose and xylogen, contact with cellulosic to increase enzyme, and reducing the inhibition of natural structure to substance transportation, pre-treatment can reduce cellulosic crystallization degree equally.
The pretreatment process of the useless branches and leaves in gardens has physical treatment process, method of chemical treatment and biological treatment at present.
(1) physical treatment process: utilize the physical means such as high temperature, ultrasonic wave, microwave can destroy to some extent the natural structure of wood fibre, change its stability.The useless branches and leaves in gardens can be realized the initial gross separation of each chemical composition after steam explosion, and it is fluffy that fiber becomes, and specific surface area increases, and hydrogen bond reduces, and structural rearrangement is conducive to improve the accessibility of reagent, intensified response performance.But physical treatment process needs specific equipment, cost is higher, and treating processes has secondary pollution.
(2) method of chemical treatment: NaOH, H
2o
2, after the examination of the chemistry such as ammoniacal liquor processes the useless branches and leaves class material in gardens, there is destruction to a certain degree fibrous, form, the crystallizing field of the useless branches and leaves in gardens, useless branches and leaves surface, gardens becomes loose, has occurred part micropore, and the biodegradability of the useless branches and leaves in gardens is improved.But chemical treatment meeting produces very serious secondary pollution.
(3) biological treatment: utilize efficient degradation bacteria to carry out fermentative processing to the useless branches and leaves in paddy rice gardens, the useless apparent siliceous nodular texture of branches and leaves in gardens comes off, and cell walls occurs damaged on a large scale, and the useless branches and leaves tissue in whole gardens becomes loose.The useless branches and leaves degradation bacteria in gardens can be decomposed various polymkeric substance in its tissue, and xylogen, Mierocrystalline cellulose and hemicellulose in useless gardens branches and leaves are decomposed into monomer or oligopolymer, improves its utility.Biological process processing cost is lower, there is no secondary pollution, but at present conventional biological treatment, microorganism used therefor transformation efficiency is lower, and the treatment time is longer, has affected application and the popularization of biological treatment.
Three, summary of the invention
The problem that the present invention need to solve is to provide a kind of useless branches and leaves starter in gardens that comprises multiple-microorganism, by utilizing efficient degradation microorganism, the useless branches and leaves in gardens are carried out to Biological Pretreatment, that improves the useless branches and leaves in gardens can utilising efficiency, thereby sets up the platform technology of the useless branches and leaves recycling in a kind of gardens.The fermentation process of the useless branches and leaves in a kind of gardens is provided simultaneously, and by useless the gardens that comprise multiple-microorganism branches and leaves starter, in the middle of the useless branches and leaves fermentation in various plants gardens, the gardens through ferment give up that branches and leaves can be used for improveing soil or as cultivation matrix.
The technical solution adopted in the present invention is:
The present invention uses multiple commercially available efficient degrading bacteria, as: excrement ghost umbrella (Coprinus sterqulinus), mould (Penicillium expansum), wood mould (Trichoderma koningii) and aspergillus (Aspergillus) ferment to the useless branches and leaves in gardens, the fermenting agent useless branches and leaves in different plants gardens that can ferment, and gardens through ferment give up that branches and leaves can be used for improveing soil or as cultivation matrix.
The gardens provided by the invention branches and leaves starter that gives up, is mainly made up of the following microbial components of weight percentage: aspergillus 0.1-0.3 part, 0.3 part of mould 0.1-, wooden mould 0.2-0.4 part and excrement ghost umbrella 0.2-0.5 part.Respectively mould to aspergillus, mould, wood, the terrible umbrella slant strains of excrement are seeded in liquid shaking bottle and are cultivated, then the seed liquor in liquid shaking bottle is inoculated in liquid seeds tank and cultivated, finally the seed liquor in liquid seeds tank being inoculated in to fermentor tank cultivates, the laggard row filter press of fermentation ends, solid-liquid separation is collected mycelium, mycelium is adsorbed with sorbent material, obtain dry bacterial powder, different strain dry bacterial powder is mixed in proportion and is the useless branches and leaves fermenting agent in gardens.
The present invention compared with prior art its beneficial effect is: in the useless branches and leaves biological treatment process in gardens, microorganism used therefor transformation efficiency is lower at present, produces foul smell stink, multiply some harmful insects, and the treatment time is longer.The useless branches and leaves fermenting agent in gardens of the nontoxic pollution-free that utilization of the present invention is made up of above-mentioned formula is processed the useless branches and leaves in gardens, does not need to inoculate other bacterial classifications, and waste can carry out from fermentation, and biological transformation ratio improves more than 5 times; And do not produce foul smell, do not multiply the insects such as mosquitos and flies.It is a kind for the treatment of process of efficient non-secondary pollution.
Four, embodiment
1. the preparation method of the useless branches and leaves fermenting agent in gardens:
(1) the useless branches and leaves fermenting agent in gardens is made up of the following microbial components of weight percentage: 0.1-0.3 part, aspergillus, 0.1-0.3 part of mould, 0.2-0.5 part, mould 0.2-0.4 part and the excrement ghost of wood umbrella, respectively aspergillus, mould, wood is mould, excrement ghost umbrella slant strains is seeded in liquid shaking bottle cultivates, then the seed liquor in liquid shaking bottle is inoculated in liquid seeds tank and cultivated, finally the seed liquor in liquid seeds tank being inoculated in to fermentor tank cultivates, the laggard row filter press of fermentation ends, solid-liquid separation is collected mycelium, mycelium is adsorbed with sorbent material, obtain dry bacterial powder, different strain is mixed and is the useless branches and leaves fermenting agent in gardens in above ratio.
2.. the application method of the useless branches and leaves starter in gardens in the useless branches and leaves in gardens are processed:
(1) the useless branches and leaves pre-treatment in gardens: it is 5-8 mm that useless gardens branches and leaves are ground into granularity, the useless branches and leaves in gardens of pulverizing mix with a certain amount of urea or ammonium sulfate, urea or ammonium sulfate usage quantity are 1-3 w/w %, make content of water in system between 60-70 w/w %, and the carbon of system/nitrogen is than being 40-65;
(2) the useless branches and leaves in fermentation gardens: the useless branches and leaves fermenting agent of useless branches and leaves material complete pre-treatment in (1) and gardens is mixed, and the total consumption of fermenting agent is the 0.6-1 w/w % of the useless branches and leaves material weight in gardens; In the time that reaching 35-45 ℃, mixed stockpile reaction starts turning, ventilate oxygenating cooling of turning every day afterwards, control temperature of charge 40 ℃ of left and right, ferment and after 10-14 days, stop turning ventilation oxygenating, in the useless branches and leaves fermenting process in gardens, pile temperature not higher than 45 ℃, finish fermentation while rubbing i.e. broken, color blackening with the hands until the useless branches and leaves in gardens are light.
Claims (3)
1. the useless branches and leaves fermenting agent in gardens, is characterized in that being made up of the following microbial components of weight percent: 0.2-0.5 part, the terrible umbrella of aspergillus 0.1-0.3 part, mould 0.1-0.3 part, wooden mould 0.2-0.4 part and excrement.
2. the preparation method of the useless branches and leaves fermenting agent in a kind of gardens according to claim 1, it is characterized in that respectively aspergillus, mould, wood is mould, excrement ghost umbrella slant strains is seeded in liquid shaking bottle cultivates, then the seed liquor in liquid shaking bottle is inoculated in liquid seeds tank and cultivated, finally the seed liquor in liquid seeds tank being inoculated in to fermentor tank cultivates, the laggard row filter press of fermentation ends, solid-liquid separation is collected mycelium, mycelium is adsorbed with sorbent material, obtain dry bacterial powder, by different strain dry bacterial powder, press 0.1-0.3 part, aspergillus, 0.1-0.3 part of mould, wooden mould 0.2-0.4 part is mixed and is the useless branches and leaves fermenting agent in gardens with the ratio of 0.2-0.5 part, excrement ghost umbrella.
3. the application method that the useless branches and leaves fermenting agent in a kind of gardens gives up in branches and leaves processing in gardens according to claim 1, is characterized in that being made up of following steps:
(1) the useless branches and leaves pre-treatment in gardens: it is 5-8 mm that useless gardens branches and leaves are ground into granularity, the useless branches and leaves in gardens of pulverizing mix with a certain amount of urea or ammonium sulfate, urea or ammonium sulfate usage quantity are 1-3 w/w %, make content of water in system between 60-70 w/w %, and the carbon of system/nitrogen is than being 40-65;
(2) the useless branches and leaves in fermentation gardens: the useless branches and leaves fermenting agent of useless branches and leaves material complete pre-treatment in (1) and gardens is mixed, and the total consumption of fermenting agent is the 0.6-1 w/w % of the useless branches and leaves material weight in gardens; In the time that reaching 35-45 ℃, mixed stockpile reaction starts turning, ventilate oxygenating cooling of turning every day afterwards, control temperature of charge 40 ℃ of left and right, ferment and after 10-14 days, stop turning ventilation oxygenating, in the useless branches and leaves fermenting process in gardens, pile temperature not higher than 45 ℃, finish fermentation while rubbing i.e. broken, color blackening with the hands until the useless branches and leaves in gardens are light.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410159682.0A CN103898029A (en) | 2014-04-21 | 2014-04-21 | Waste garden branch and leaf fermenting bacterial preparation as well as preparation method and application thereof |
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| CN201410159682.0A CN103898029A (en) | 2014-04-21 | 2014-04-21 | Waste garden branch and leaf fermenting bacterial preparation as well as preparation method and application thereof |
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| CN103898029A true CN103898029A (en) | 2014-07-02 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106478166A (en) * | 2016-09-22 | 2017-03-08 | 北京林业大学 | The processing method for promoting intermediate cutting residue to decompose |
| CN106495944A (en) * | 2016-11-01 | 2017-03-15 | 金陵科技学院 | A kind of biofermentation method of plant refuse and the application of products therefrom |
| CN106588346A (en) * | 2016-12-15 | 2017-04-26 | 岑溪市安安农业科技发展有限公司 | Production method of pitaya ferment fertilizer |
| CN111499447A (en) * | 2020-05-12 | 2020-08-07 | 广东碧然美景观艺术有限公司 | Modification method of garden waste fermented organic fertilizer |
| CN114868581A (en) * | 2022-05-20 | 2022-08-09 | 莫稳方 | Forest tending and felling residue treatment method |
Citations (2)
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| CN1724485A (en) * | 2005-06-15 | 2006-01-25 | 湖南大学 | Active additive for promoting agricultural waste material compost decomposition and its application |
| CN102453676A (en) * | 2010-10-18 | 2012-05-16 | 嘉兴学院 | Straw starter and use of the straw starter in straw fermentation |
-
2014
- 2014-04-21 CN CN201410159682.0A patent/CN103898029A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1724485A (en) * | 2005-06-15 | 2006-01-25 | 湖南大学 | Active additive for promoting agricultural waste material compost decomposition and its application |
| CN102453676A (en) * | 2010-10-18 | 2012-05-16 | 嘉兴学院 | Straw starter and use of the straw starter in straw fermentation |
Non-Patent Citations (2)
| Title |
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| 王安娇: "城市园林植物废枝叶分解利用研究", 《中国优秀硕士学位论文全文数据库》 * |
| 王磊等: "粪鬼伞菌丝体提取液的抗菌活性", 《食品与生物技术学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106478166A (en) * | 2016-09-22 | 2017-03-08 | 北京林业大学 | The processing method for promoting intermediate cutting residue to decompose |
| CN106478166B (en) * | 2016-09-22 | 2020-02-14 | 北京林业大学 | Treatment method for promoting decomposition of intermediate cutting residues |
| CN106495944A (en) * | 2016-11-01 | 2017-03-15 | 金陵科技学院 | A kind of biofermentation method of plant refuse and the application of products therefrom |
| CN106588346A (en) * | 2016-12-15 | 2017-04-26 | 岑溪市安安农业科技发展有限公司 | Production method of pitaya ferment fertilizer |
| CN111499447A (en) * | 2020-05-12 | 2020-08-07 | 广东碧然美景观艺术有限公司 | Modification method of garden waste fermented organic fertilizer |
| CN114868581A (en) * | 2022-05-20 | 2022-08-09 | 莫稳方 | Forest tending and felling residue treatment method |
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Application publication date: 20140702 |