CN115500238B - Method for improving phosphogypsum storage yard soil by using waste straw - Google Patents
Method for improving phosphogypsum storage yard soil by using waste straw Download PDFInfo
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- PASHVRUKOFIRIK-UHFFFAOYSA-L calcium sulfate dihydrate Chemical compound O.O.[Ca+2].[O-]S([O-])(=O)=O PASHVRUKOFIRIK-UHFFFAOYSA-L 0.000 title claims abstract description 101
- 239000002689 soil Substances 0.000 title claims abstract description 79
- 238000000034 method Methods 0.000 title claims abstract description 50
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- 241000187747 Streptomyces Species 0.000 claims description 11
- 241000499912 Trichoderma reesei Species 0.000 claims description 11
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- 241000146399 Ceriporiopsis Species 0.000 description 7
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 7
- 244000005700 microbiome Species 0.000 description 7
- 239000011574 phosphorus Substances 0.000 description 7
- 229910052698 phosphorus Inorganic materials 0.000 description 7
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- 108020004465 16S ribosomal RNA Proteins 0.000 description 4
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
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- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 2
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- 229920001817 Agar Polymers 0.000 description 1
- 108010010803 Gelatin Proteins 0.000 description 1
- 240000004296 Lolium perenne Species 0.000 description 1
- 241001453830 Pteridium Species 0.000 description 1
- 241000737257 Pteris <genus> Species 0.000 description 1
- 240000001717 Vaccinium macrocarpon Species 0.000 description 1
- 235000012545 Vaccinium macrocarpon Nutrition 0.000 description 1
- 235000002118 Vaccinium oxycoccus Nutrition 0.000 description 1
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- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
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- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 1
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- OJMIONKXNSYLSR-UHFFFAOYSA-N phosphorous acid Chemical compound OP(O)O OJMIONKXNSYLSR-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Inorganic Chemistry (AREA)
- Soil Sciences (AREA)
- Soil Conditioners And Soil-Stabilizing Materials (AREA)
Abstract
The invention relates to a method for improving phosphogypsum storage yard soil by utilizing waste straws, which specifically comprises the following steps: s1: mixing phosphogypsum powder and alkaline soil according to a certain mass ratio to obtain pretreated phosphogypsum; s2: mixing the pretreated phosphogypsum with straw powder, and spraying a composite bacterial liquid to obtain a primary soil-like matrix; s3: introducing pioneer plants into the primary soil-like matrix to obtain a medium-grade soil-like matrix; s4: and introducing common plants into the medium soil-like matrix to obtain the high soil-like matrix. The invention has the beneficial effects that phosphogypsum storage yard can be subjected to soil formation, and meanwhile, the agricultural waste straw is efficiently utilized, so that the problem of piling up a large amount of phosphogypsum and the agricultural waste straw is solved, and the invention has great practical significance for environmental ecological protection and resource recycling integration and has the advantages of simple process, high treatment efficiency, green ecology and low cost.
Description
Technical Field
The invention relates to the technical field of resource integrated utilization and ecological restoration, in particular to a method for improving phosphogypsum storage yard soil by utilizing waste straws.
Background
Phosphogypsum is a byproduct obtained by decomposing phosphorite with sulfuric acid in the wet-process phosphoric acid production process, and contains harmful impurities such as phosphorus, fluorine and the like. A large amount of phosphogypsum is still piled up in China, so that a large amount of land is occupied by piling up the phosphogypsum, and the long-time piling up of the phosphogypsum can cause soluble phosphorus, fluorine and the like contained in the phosphogypsum to enter soil, surface water and underground water, thereby causing serious environmental problems. Meanwhile, phosphogypsum is different from common soil and conventional metal tailings, has great discreteness, and has a vertical permeability coefficient larger than a horizontal permeability coefficient, so that karst cave and karst ditch are easy to appear in a storage yard, and further, the safety problem is caused. Aiming at the environmental problems caused by the release of soluble phosphorus and soluble fluorine in phosphogypsum yards, the harmless treatment, the soil improvement and the ecological utilization of phosphogypsum are vital to the healthy development of phosphorus chemical industry chains under the background of increasingly strict environmental protection supervision and energy structure transformation.
Aiming at the harmless treatment of phosphogypsum, the research reports at present still mainly concentrate on utilizing and improving the traditional method for phosphogypsum treatment, and the traditional method has the defects of single benefit, complex process, higher energy consumption, secondary pollution and the like. At present, research on realizing phosphogypsum soil formation by combining phosphogypsum and agricultural straw with high-efficiency biomass degrading bacteria is not seen.
Disclosure of Invention
The invention aims to solve the technical problem of providing a method for improving phosphogypsum storage yard soil by using waste straws, and aims to solve the problem in the prior art.
The technical scheme for solving the technical problems is as follows:
the method for improving the phosphogypsum storage yard soil by utilizing the waste straws specifically comprises the following steps:
s1: mixing phosphogypsum powder and alkaline soil according to a certain mass ratio to obtain pretreated phosphogypsum;
s2: mixing the pretreated phosphogypsum with straw powder, and spraying a composite bacterial liquid to obtain a primary soil-like matrix;
s3: introducing pioneer plants into the primary soil-like matrix to obtain a medium-grade soil-like matrix;
s4: and introducing common plants into the medium soil-like matrix to obtain the high soil-like matrix.
The beneficial effects of the invention are as follows: the invention can soil phosphogypsum storage yard, efficiently utilizes agricultural waste straw, solves the problem of piling up a large amount of phosphogypsum and agricultural waste straw, has great practical significance for environmental ecological protection and resource recycling integration, and has the advantages of simple process, high treatment efficiency, green ecology and low cost.
On the basis of the technical scheme, the invention can be improved as follows.
Further, the step S1 is preceded by the step S0, and the preparation of the composite bacterial liquid specifically comprises the following steps:
s01: respectively carrying out enrichment culture on the separated and purified different strains in a liquid culture medium to obtain enrichment culture solutions of different cellulose degrading bacteria;
s02: and (3) respectively carrying out acclimation culture for the generation times on the different enrichment culture solutions to obtain acclimation culture solutions of different cellulose degrading bacteria, and mixing the different acclimation culture solutions according to a certain volume ratio to obtain a composite bacterial solution.
The method has the beneficial effects that the process is simple, and the composite bacterial liquid can be rapidly obtained.
Further, the step S01 specifically includes the following steps:
s011: collecting saprophyte and surrounding soil samples, filling the saprophyte and the surrounding soil samples into a container according to a mass ratio (1-2) of 1 with sterile water, mixing and precipitating to obtain supernatant;
s012: filling the supernatant and the enrichment medium into a container according to the volume ratio (0.1-0.4): 1, and placing the container into a constant temperature shaking table at 25-35 ℃ for shaking culture at the rotating speed of 160-200 rpm to obtain an enrichment bacterial liquid;
s013: uniformly mixing the enriched bacterial liquid and a cellulolytic culture medium according to the volume ratio of (5-10), and culturing for 3-7 days at a constant temperature shaking table of 26-32 ℃ and a rotating speed of 160-200 rpm to obtain a cellulose composite bacterial liquid;
s014: inoculating the cellulose composite bacterial liquid into a cellulolytic culture medium, inversely culturing for 3-5 days at 26-32 ℃ to obtain bacterial colonies of cellulose degrading bacteria, selecting single bacterial colonies with different forms, purifying and culturing for 3-5 days at 26-32 ℃ by using a plate drawing line separation culture mode to obtain purified cellulolytic bacterial strains;
s015: inoculating the purified cellulose degrading strain into a cellulose degrading culture medium, and reversely culturing for 3-5 days at 28-30 ℃ to screen the cellulose degrading strain with transparent rings;
s016: inoculating the screened cellulose degradation strain into a liquid enzyme production culture medium, placing the liquid enzyme production culture medium in a constant temperature shaking table at 26-32 ℃ for shaking culture at a rotating speed of 160-200 rpm for 5-7 days, and comparing the total cellulase activity in the culture solution to obtain high-efficiency cellulose degradation bacteria;
s017: separating and screening strains of Ceriporiopsis, streptomyces, bacillus subtilis, trichoderma reesei, chaetomium and Bacillus amyloliquefaciens, and culturing the strains screened by the expansion culture medium in a constant temperature shaking table at 26-32deg.C at 180-220 rpm for 5-7 days to obtain different enrichment culture solutions after expansion culture.
The method has the beneficial effects that the process is simple, different enrichment culture solutions can be obtained quickly, and convenience and rapidness are realized.
Further, the step S02 specifically includes the following steps:
s021: inoculating the enriched culture solution of different cellulose degrading bacteria into domestication culture medium containing straw powder and phosphogypsum, and subculturing at 26-32deg.C for 4-7 times until the total concentration of cellulose degrading bacteria in each bacterial solution is 10 7 -10 9 Obtaining different cellulose degrading bacteria liquid by using/mL;
s022: and (3) respectively carrying out expansion culture on different cellulose degrading bacterial solutions, and mixing the bacterial solutions according to a certain volume ratio to prepare the composite bacterial solution.
The method has the beneficial effects that the process is simple, different cellulose degrading bacterial solutions can be obtained by using the enrichment culture solution, and then the different cellulose degrading bacterial solutions are used for carrying out expansion culture, so that the composite bacterial solution is obtained.
Further, in S1, phosphogypsum: mixing alkaline soil according to a mass ratio of 1 (2-4).
The adoption of the further scheme has the beneficial effects that the proportion is proper, so that phosphogypsum and alkaline soil can be better utilized, waste is treated by waste, and the energy is saved and the environment is protected.
Further, the step S2 specifically includes the following steps:
s21: the preparation method comprises the following steps of (1) pre-treating phosphogypsum and waste straw powder according to a mass ratio of 1: (1-3) placing the mixture into a stirrer, and mixing the mixture for 2-10 hours at a rotating speed of 40-60 revolutions per minute to obtain the organic phosphogypsum;
s22: the compound bacterial liquid is treated by 7-15L/(h.m) 2 ) The spraying speed of the water-based composite material is intermittently sprayed in the organic phosphogypsum for 1h at intervals of 1-2h, and the primary soil-like matrix is obtained after 15-60 days of growth and field planting.
The method has the beneficial effects that the process is simple, and the components in the primary soil are decomposed by utilizing the strain in the composite bacterial liquid so as to obtain the primary soil-like matrix.
Further, the step S3 specifically includes the following steps:
and (2) planting pioneer plants in the primary soil-like matrix in the step (S2), and obtaining the intermediate soil-like matrix after 1-5 rounds of field planting growth.
The adoption of the further scheme has the beneficial effects that the selection is reasonable, the pioneer plant has the characteristics of strong vitality, high growth speed, strong environmental resistance, developed root system and the like, and the ecological function of phosphogypsum can be comprehensively improved through the physiological activities of the root system.
Further, in the step S3, the pioneer plant comprises one or more of a plant of Pteridium multiflorum and Lolium perenne.
The adoption of the further scheme has the advantages of reasonable selection, common varieties and convenient material acquisition.
Further, the step S4 specifically includes the following steps: and (3) planting the common plants in the medium-class soil matrix in the step (S3), and obtaining the high-class soil matrix after 1-5 rounds of field planting growth.
The adoption of the further scheme has the beneficial effects of simple process and reasonable design, and further improves the nutrition of the soil-like matrix.
Further, in the step S4, the common plants include one or more of fescue, bluegrass, alfalfa and centella asiatica.
The adoption of the further scheme has the beneficial effects that the common plants can promote plant growth and exert physiological functions, promote the formation of aggregates and promote mineral nutrients.
Drawings
FIG. 1 is a process flow diagram of the present invention.
Detailed Description
It should be noted that, without conflict, the embodiments of the present invention and features of the embodiments may be combined with each other.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first", "a second", etc. may explicitly or implicitly include one or more such feature. In the description of the present invention, unless otherwise indicated, the meaning of "a plurality" is two or more.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art in a specific case.
The invention will be described in detail below with reference to the drawings in connection with embodiments.
Example 1
As shown in fig. 1, the embodiment provides a method for improving the soil of phosphogypsum storage yard by using waste straw, which specifically comprises the following steps:
s1: mixing phosphogypsum powder and alkaline soil according to a certain mass ratio to obtain pretreated phosphogypsum;
s2: mixing the pretreated phosphogypsum with straw powder, and spraying a composite bacterial liquid to obtain a primary soil-like matrix;
s3: introducing pioneer plants into the primary soil-like matrix to obtain a medium-grade soil-like matrix;
s4: and introducing common plants into the medium soil-like matrix to obtain the high soil-like matrix.
The phosphogypsum storage yard can be subjected to soil formation, meanwhile, the agricultural waste straw is efficiently utilized, the problem of piling up a large amount of phosphogypsum and the agricultural waste straw is solved, the phosphogypsum storage yard has great practical significance for environmental ecological protection and resource recycling integration, and the phosphogypsum storage yard has the advantages of being simple in process, high in treatment efficiency, green and ecological and low in cost.
Example 2
Based on embodiment 1, in this embodiment, S0 is further included before S1, and the preparation of the composite bacterial liquid specifically includes the following steps:
s01: respectively carrying out enrichment culture on the separated and purified different strains in a liquid culture medium to obtain enrichment culture solutions of different cellulose degrading bacteria;
s02: and (3) respectively carrying out acclimation culture for the generation times on the different enrichment culture solutions to obtain acclimation culture solutions of different cellulose degrading bacteria, and mixing the different acclimation culture solutions according to a certain volume ratio to obtain a composite bacterial solution.
The method has simple process and can rapidly obtain the composite bacterial liquid.
Example 3
On the basis of embodiment 2, in this embodiment, the step S01 specifically includes the following steps:
s011: collecting saprophyte and surrounding soil samples, filling the saprophyte and the surrounding soil samples into a container according to a mass ratio (1-2) of 1 with sterile water, mixing and precipitating to obtain supernatant;
s012: filling the supernatant and the enrichment medium into a container according to the volume ratio (0.1-0.4): 1, and placing the container into a constant temperature shaking table at 25-35 ℃ for shaking culture at the rotating speed of 160-200 rpm to obtain an enrichment bacterial liquid;
s013: uniformly mixing the enriched bacterial liquid and a cellulolytic culture medium according to the volume ratio of (5-10), and culturing for 3-7 days at a constant temperature shaking table of 26-32 ℃ and a rotating speed of 160-200 rpm to obtain a cellulose composite bacterial liquid;
s014: inoculating the cellulose composite bacterial liquid into a cellulolytic culture medium, inversely culturing for 3-5 days at 26-32 ℃ to obtain bacterial colonies of cellulose degrading bacteria, selecting single bacterial colonies with different forms, purifying and culturing for 3-5 days at 26-32 ℃ by using a plate drawing line separation culture mode to obtain purified cellulolytic bacterial strains;
s015: inoculating the purified cellulose degrading strain into a cellulose degrading culture medium, and reversely culturing for 3-5 days at 28-30 ℃ to screen the cellulose degrading strain with transparent rings;
s016: inoculating the screened cellulose degradation strain into a liquid enzyme production culture medium, placing the liquid enzyme production culture medium in a constant temperature shaking table at 26-32 ℃ for shaking culture at a rotating speed of 160-200 rpm for 5-7 days, and comparing the total cellulase activity in the culture solution to obtain high-efficiency cellulose degradation bacteria;
s017: separating and screening strains of Ceriporiopsis, streptomyces, bacillus subtilis, trichoderma reesei, chaetomium and Bacillus amyloliquefaciens, and culturing the strains screened by the expansion culture medium in a constant temperature shaking table at 26-32deg.C at 180-220 rpm for 5-7 days to obtain different enrichment culture solutions after expansion culture.
The method has simple process, can rapidly obtain different enrichment culture solutions, and is convenient and rapid.
Preferably, in this example, the high-efficiency cellulose degrading bacteria are subjected to 16S rDNA identification and NCBI comparison (molecular identification), and the isolated and screened strains are determined after comparison as follows: ceriporiopsis, streptomyces, bacillus subtilis, trichoderma reesei, chaetomium, and Bacillus amyloliquefaciens.
Preferably, in this embodiment, the enrichment medium comprises the following components: 2g/L K 2 HPO 4 、1.4g/L (NH 4 ) 2 SO 4 、0.3g/L Mg SO 4 ·7H 2 O、0.3g/L Ca Cl 2 、5mg/L FeSO 4 ·7H 2 O、1.6 mg/L MnSO 4 、1.7mg/L ZnSO 4 2mg/L CoCl 2 The solvent is sterile water.
Preferably, in this embodiment, the components of the above-mentioned cellulolytic medium are: 10g/L sodium carboxymethyl cellulose, 2g/L K 2 HPO 4 、0.5g/L MgSO 4 0.25g/L Congo red, 16g/L agar and 3g/L gelatin, and the solvent is sterile water.
Preferably, in this embodiment, the components of the liquid enzyme-producing medium are: 5-10g/L sodium carboxymethyl cellulose, 1-2g/L NH 4 Cl、0.5-1g/L MgSO 4 ·7H 2 O、1-2g/L KH 2 PO 4 And 1-2g/L yeast extract powder, and the solvent is sterile water.
Preferably, in this embodiment, the components of the expansion medium are: 10g/L of tryptone, 5g/L of yeast extract and 10g/L of sodium chloride, and the solvent is sterile water.
Example 4
In this embodiment, on the basis of any one of embodiments 2 to 3, the step S02 specifically includes the following steps:
s021: inoculating the enriched culture solution of different cellulose degrading bacteria into domestication culture medium containing straw powder and phosphogypsum, and subculturing at 26-32deg.C for 4-7 times until the total concentration of cellulose degrading bacteria in each bacterial solution is 10 7 -10 9 Obtaining different cellulose degrading bacteria liquid by the method per mL;
s022: and (3) respectively carrying out expansion culture on different cellulose degrading bacterial solutions, and mixing the bacterial solutions according to a certain volume ratio to prepare the composite bacterial solution.
The method has simple process, can obtain different cellulose degrading bacterial solutions by using the enrichment culture solution, and then uses the different cellulose degrading bacterial solutions for carrying out expansion culture to obtain the composite bacterial solution.
Preferably, in this embodiment, the acclimated liquid medium comprises the following components: 1.0g/L KH 2 PO 4 、 0.3g/L MgSO 4 、0.1g/L NaCl、2.5g/L NaNO 3 、0.1/L CaCl 2 ·6H 2 O、0.01g/L FeCl 3 20g/L straw powder and 10g/L phosphogypsum powder, and the solvent is sterile water.
Preferably, in the embodiment, the bacterial strains of Ceriporiopsis entomorpha, streptomyces, bacillus subtilis, trichoderma reesei, chaetomium and Bacillus amyloliquefaciens in the above step S022 are prepared into the composite bacterial liquid according to the volume ratio of 1 (1-3) (2-4) (1-3) (2-4).
Example 5
Based on the above embodiments, in this embodiment, in S1, phosphogypsum and alkaline soil are mixed according to a mass ratio of 1 (2-4).
The proportion is suitable, so that phosphogypsum and alkaline soil can be better utilized, waste is treated by waste, and the method is energy-saving and environment-friendly.
Example 6
Based on the foregoing embodiments, in this embodiment, the step S2 specifically includes the following steps:
s21: the preparation method comprises the following steps of (1) pre-treating phosphogypsum and waste straw powder according to a mass ratio of 1: (1-3) placing the mixture into a stirrer, and mixing the mixture for 2-10 hours at a rotating speed of 40-60 revolutions per minute to obtain the organic phosphogypsum;
s22: the compound bacterial liquid is treated by 7-15L/(h.m) 2 ) The spraying speed of the water-based composite material is intermittently sprayed in the organic phosphogypsum for 1h at intervals of 1-2h, and the primary soil-like matrix is obtained after 15-60 days of growth and field planting.
The method has simple process, and utilizes the strain in the composite bacterial liquid to decompose the components in the organic phosphogypsum so as to obtain the primary soil-like matrix.
Example 7
Based on the foregoing embodiments, in this embodiment, the step S3 specifically includes the following steps:
and (2) planting pioneer plants in the primary soil-like matrix in the step (S2), and obtaining the intermediate soil-like matrix after 1-5 rounds of field planting growth.
The scheme is reasonable in selection, and the pioneer plant has the characteristics of being strong in vitality, high in growth speed, strong in environmental resistance, developed in root system and the like, and can comprehensively improve the phosphogypsum ecological function through physiological activities of the root system.
Example 8
In this embodiment, based on embodiment 7, in S3, the pioneer plant includes one or more of pteris multifida and ryegrass.
The scheme has reasonable selection, common varieties and convenient material taking.
Example 9
Based on the foregoing embodiments, in this embodiment, the step S4 specifically includes the following steps:
and (3) planting the common plants in the medium-class soil matrix in the step (S3), and obtaining the high-class soil matrix after 1-5 rounds of field planting growth.
The scheme has simple process and reasonable design, and further improves the nutrition of soil-like matrix.
Example 10
In this embodiment, based on embodiment 9, in S4, the common plant includes one or more of fescue, bluegrass, alfalfa and cranberry.
The common plants can promote plant growth and play physiological functions, promote aggregate formation and promote mineral nutrient.
The embodiments of the pioneer plant, the common plant, and the like are as follows:
example 11
A method for improving phosphogypsum storage yard soil by utilizing waste straws comprises the following steps:
1) Collecting saprophyte and surrounding soil samples, filling the saprophyte and the surrounding soil samples into a container according to the mass ratio of the saprophyte to the sterile water of 1:1, and mixing and precipitating to obtain supernatant;
loading the supernatant and the enrichment medium into a container according to the volume ratio of 0.2:1, and placing the container into a constant temperature shaking table at 30 ℃ for shaking culture at the rotating speed of 160 revolutions per minute to obtain an enrichment bacterial liquid;
uniformly mixing the enriched bacterial liquid and the cellulolytic culture medium according to the volume ratio of 2:5, and then placing the mixture in a constant-temperature shaking table at 28 ℃ to culture for 3 days at the rotating speed of 180 revolutions per minute to obtain cellulose composite bacterial liquid;
inoculating the composite bacterial liquid obtained after the culture into a cellulolytic culture medium, inversely culturing for 3 days at the temperature of 28 ℃ to obtain bacterial colonies of cellulose degrading bacteria, selecting single bacterial colonies with different forms, purifying and culturing for 3 days at the temperature of 30 ℃ by using a plate drawing line separation culture mode, and thus obtaining purified cellulolytic bacterial strains;
inoculating the purified cellulose degrading strain into a cellulose degrading culture medium, and culturing for 3 days at 30 ℃ in an inverted way to screen the cellulose degrading strain with transparent rings;
inoculating the screened cellulose degradation strain into a liquid enzyme production culture medium, placing the liquid enzyme production culture medium in a shaking table at a constant temperature of 28 ℃ for shaking culture at a rotating speed of 180 revolutions per minute for 5 days, comparing the total cellulase activity in the culture solution, and selecting the strain with the highest index to determine the strain as efficient cellulose degradation bacteria;
carrying out 16S rDNA identification on the efficient cellulose degrading bacteria, and determining that the isolated and screened bacterial strains are: ceriporiopsis, streptomyces, bacillus subtilis, trichoderma reesei, chaetomium, and Bacillus amyloliquefaciens.
And (3) performing expansion culture on the high-efficiency cellulose degrading bacteria, and performing shaking culture at a constant temperature of 180 revolutions per minute for 5 days at the temperature of 28 ℃ to obtain bacterial liquid after expansion culture.
2) Inoculating different cellulose degrading bacteria liquid into domestication culture medium containing straw powder and phosphogypsum, subculturing and culturing at 28deg.C for 4 times until the total concentration of cellulose degrading bacteria in each bacteria liquid is 10 7 individual/mL;
performing amplification culture on selected bacterial colonies of the domesticated cermopsis, streptomyces, bacillus subtilis, trichoderma reesei, chaetomium and bacillus amyloliquefaciens, and preparing a composite bacterial solution according to the volume ratio of 1:2:4:3:2:4.
3) Uniformly mixing phosphogypsum and alkaline soil according to a mass ratio of 1:3, and obtaining the pretreated phosphogypsum.
4) The preparation method comprises the following steps of (1) pre-treating phosphogypsum and waste straw powder according to a mass ratio of 1:3, placing the mixture into a stirrer, and mixing the mixture for 6 hours at a rotating speed of 45 revolutions per minute to obtain the organic phosphogypsum;
mixing the screened and domesticated efficient cellulose degrading bacteria according to the volume ratio of 1:2:4:3:2:4 to prepare a composite bacterial liquid, and spraying the composite bacterial liquid into the organic phosphogypsum;
the spraying speed of the composite bacterial liquid is 7L/(h.m) 2 ) The spraying mode is intermittent, and spraying is carried out for 1h at each interval of 1h; the primary soil-like matrix is obtained after the growth and the field planting of the microorganism in the composite bacterial liquid for 60 days.
5) Planting pioneer plant Pteris multiflorus in the primary soil matrix, and providing corresponding growth environment and nutrients. The obtained primary soil-like matrix is stabilized for 5 days at room temperature, so that the internal physicochemical conditions reach a certain balance. Planting is directly performed in the primary soil-like matrix, and the germination rate of seeds is also calculated. The soil amount of each group divided into 5 groups is 10kg, the seed sowing amount of each group of the pteris multifida is 9, 11, 13, 14 and 15, and the germination rate is counted after 7 days. After the plant grows for two periods of time, the medium class soil matrix is obtained.
6) Plants such as festuca arundinacea, bluegrass, alfalfa, and radix et rhizoma zingiberis are planted in the medium-class soil matrix. 10 seeds and 3 hundred vein root seeds are sown, compound fertilizer is applied once every three days, and the germination rate of the seeds is calculated after 7 days. And obtaining the high-grade soil-like matrix after the planting of the plants for three period times.
Table 1 shows physical and chemical indexes of the soil after the improvement of the present example
From the data in table 1, it can be seen that the nutrient components in the primary soil-like matrix, the intermediate soil-like matrix and the high soil-like matrix are significantly improved, and the nutrient components of the soil in the primary soil-like matrix, the intermediate soil-like matrix and the high soil-like matrix are sequentially improved.
Example 12
A method for improving phosphogypsum storage yard soil by utilizing waste straws comprises the following steps:
1) Collecting saprophyte and surrounding soil samples, filling the saprophyte and the surrounding soil samples into a container according to a weight ratio of 2:1 with sterile water, and mixing and precipitating to obtain supernatant;
supernatant and enrichment medium are mixed according to the volume ratio of 0.4:1, placing the container into a container, and placing the container into a constant temperature shaking table at 30 ℃ for shaking culture at a rotating speed of 200 revolutions per minute to obtain enriched bacterial liquid;
uniformly mixing the enriched bacterial liquid and the cellulolytic culture medium according to the volume ratio of 2:7, and then placing the mixture in a constant-temperature shaking table at 28 ℃ to culture for 5 days at the rotating speed of 160 revolutions per minute to obtain cellulose composite bacterial liquid;
inoculating the composite bacterial liquid obtained after the culture into a cellulolytic culture medium, inversely culturing for 3 days at 30 ℃ to obtain bacterial colonies of cellulose degrading bacteria, selecting single bacterial colonies with different forms, and purifying and culturing for 4 days at 28 ℃ by using a plate drawing line separation culture mode to obtain purified cellulolytic bacterial strains;
inoculating the purified cellulose degrading strain into a cellulose degrading culture medium, and culturing for 3 days at 30 ℃ in an inverted way to screen the cellulose degrading strain with transparent rings;
inoculating the screened cellulose degradation strain into a liquid enzyme production culture medium, placing the liquid enzyme production culture medium in a shaking table at a constant temperature of 30 ℃ for shaking culture at a rotating speed of 200 revolutions per minute for 7 days, comparing the total cellulase activity in the culture solution, and selecting the strain with the highest index to determine the strain as efficient cellulose degradation bacteria;
carrying out 16S rDNA identification on the efficient cellulose degrading bacteria, and determining that the isolated and screened bacterial strains are: ceriporiopsis, streptomyces, bacillus subtilis, trichoderma reesei, chaetomium, and Bacillus amyloliquefaciens;
and (3) performing expansion culture on the high-efficiency cellulose degrading bacteria, and placing the bacteria in a 30 ℃ constant-temperature shaking table for 5 days at the rotating speed of 200 rpm to obtain bacterial liquid after the expansion culture.
2) Inoculating different cellulose degrading bacteria liquid into domestication culture medium containing straw powder and phosphogypsum, subculturing and culturing for 6 times at 28deg.C,until the total concentration of cellulose degrading bacteria in each bacterial liquid is 10 7 individual/mL;
performing amplification culture on selected bacterial colonies of domesticated cermopsis, streptomyces, bacillus subtilis, trichoderma reesei, chaetomium and bacillus amyloliquefaciens, and preparing a composite bacterial solution according to the volume ratio of 1:2:4:3:2:4.
3) And fully and uniformly mixing phosphogypsum and alkaline soil according to the mass ratio of 1:2, and obtaining the pretreated phosphogypsum.
4) The preparation method comprises the following steps of (1) pre-treating phosphogypsum and waste straw powder according to a mass ratio of 1:3, placing the mixture into a stirrer, and mixing the mixture for 4 hours at a rotating speed of 45 revolutions per minute to obtain the organic phosphogypsum;
mixing the screened and domesticated efficient cellulose degrading bacteria according to the volume ratio of 1:2:4:3:2:4 to prepare a composite bacterial liquid, and spraying the composite bacterial liquid into the organic phosphogypsum;
the spraying speed of the composite bacterial liquid is 15L/(h.m2), the spraying mode is intermittent, and the spraying is carried out for 1h at intervals of 2 h. The microorganism in the composite bacterial liquid is grown and planted for 30 days to obtain a primary soil-like matrix.
5) In the primary soil-like matrix, the pioneer plant ryegrass is planted and provides its corresponding growth environment and nutrients. The obtained primary soil-like matrix is stabilized for 5 days at room temperature, so that the internal physicochemical conditions reach a certain balance.
The seeds are planted in the primary soil-like matrix directly, and the germination rate of the seeds is calculated. The soil quantity of each group divided into 5 groups is 7kg, 9kg, 10kg, 12kg and 15kg, the seed quantity of each group of ryegrass is 9, 11, 13, 14 and 15 grains, and after 7 days, the germination rate is counted and the medium-grade soil matrix is obtained after the field planting of three period times of plant growth.
6) Plants such as festuca arundinacea, bluegrass, alfalfa, and radix et rhizoma zingiberis are planted in the medium-class soil matrix. Sowing 13 seeds and 3 hundred vein root seeds, applying compound fertilizer once every three days, and calculating the germination rate after 7 days. After 3-period planting, the high-grade soil-like matrix is obtained.
Table 2 shows physical and chemical indexes of the soil after the improvement of the present example
| Project | Phosphogypsum group | Primary soil-like matrix | Medium class soil matrix | Advanced soil-like matrix |
| pH value of | 1.4 | 4.47 | 5.12 | 7.57 |
| Soil Total porosity (%) | / | 34％ | 44％ | 57％ |
| Nitrogen (%) | / | 0.07％ | 0.22％ | 0.30％ |
| Whole phosphorus (g/kg) | / | 0.47 | 0.59 | 0.79 |
| Whole potassium (g/kg) | / | 11 | 14 | 21 |
| Humic acid | / | 0.64％ | 0.17％ | 2.01％ |
From the data in table 2, it can be seen that the nutrient components in the primary soil-like matrix, the intermediate soil-like matrix and the high soil-like matrix are significantly improved, and the nutrient components of the soil in the primary soil-like matrix, the intermediate soil-like matrix and the high soil-like matrix are sequentially improved.
Example 13
A method for improving phosphogypsum storage yard soil by utilizing waste straws comprises the following steps:
1) Collecting saprophyte and surrounding soil samples, filling the saprophyte and the surrounding soil samples into a container according to a weight ratio of 2:1 with sterile water, and mixing and precipitating to obtain supernatant;
supernatant and enrichment medium are mixed according to the volume ratio of 0.3:1, placing the container into a container, and placing the container into a constant temperature shaking table at 30 ℃ for shaking culture at a rotating speed of 200 revolutions per minute to obtain enriched bacterial liquid;
uniformly mixing the enriched bacterial liquid and the cellulolytic culture medium according to the volume ratio of 2:10, and then placing the mixture in a constant-temperature shaking table at 26 ℃ to culture for 5 days at the rotating speed of 200 rpm to obtain cellulose composite bacterial liquid;
inoculating the composite bacterial liquid obtained after the culture into a cellulolytic culture medium, inversely culturing for 3 days at the temperature of 32 ℃ to obtain bacterial colonies of cellulose degrading bacteria, selecting single bacterial colonies with different forms, and purifying and culturing for 3 days at the temperature of 32 ℃ by using a plate drawing line separation culture mode to obtain purified cellulolytic bacterial strains;
inoculating the purified cellulose degrading strain into a cellulose degrading culture medium, and culturing for 3 days at 30 ℃ in an inverted way to screen the cellulose degrading strain with transparent rings;
inoculating the screened cellulose degradation strain into a liquid enzyme production culture medium, placing the liquid enzyme production culture medium in a shaking table at a constant temperature of 30 ℃ for shaking culture at a rotating speed of 180 revolutions per minute for 7 days, comparing the total cellulase activity in the culture solution, and selecting the strain with the highest index to determine the strain as efficient cellulose degradation bacteria;
carrying out 16S rDNA identification on the efficient cellulose degrading bacteria, and determining that the isolated and screened bacterial strains are: ceriporiopsis, streptomyces, bacillus subtilis, trichoderma reesei, chaetomium, and Bacillus amyloliquefaciens;
and (3) performing expansion culture on the high-efficiency cellulose degrading bacteria, and placing the bacteria in a 30 ℃ constant-temperature shaking table for 5 days at a rotating speed of 180 rpm to obtain bacterial liquid after the expansion culture.
2) Inoculating different cellulose degrading bacteria liquid into domestication culture medium containing straw powder and phosphogypsum, subculturing and culturing at 28deg.C for 6 times until the total concentration of cellulose degrading bacteria in each bacteria liquid is 10 7 individual/mL;
performing amplification culture on selected bacterial colonies of domesticated cermopsis, streptomyces, bacillus subtilis, trichoderma reesei, chaetomium and bacillus amyloliquefaciens, and preparing a composite bacterial solution according to the volume ratio of 1:3:4:3:1:4.
3) Phosphogypsum and alkaline soil are mixed according to the mass ratio of 1:3, fully and uniformly mixing to obtain the pretreated phosphogypsum.
4) The preparation method comprises the following steps of (1) pre-treating phosphogypsum and waste straw powder according to a mass ratio of 1:3, placing the mixture into a stirrer, and mixing the mixture for 8 hours at a rotating speed of 45 revolutions per minute to obtain the organic phosphogypsum;
mixing the screened and domesticated efficient cellulose degrading bacteria according to the volume ratio of 1:3:3:3:2:4 to prepare a composite bacterial liquid, and spraying the composite bacterial liquid into the pretreated phosphogypsum;
the spraying speed of the composite bacterial liquid is 10L/(h.m2), the spraying mode is intermittent, and the spraying is carried out for 1h at intervals of 2 h. The microorganism in the composite bacterial liquid is grown and planted for 45 days to obtain a primary soil-like matrix.
5) In the primary soil-like matrix, the pioneer plant ryegrass is planted and provides its corresponding growth environment and nutrients. The obtained primary soil-like matrix is stabilized for 5 days at room temperature, so that the internal physicochemical conditions reach a certain balance.
The seeds are planted in the primary soil-like matrix directly, and the germination rate of the seeds is calculated. The soil quantity of each group divided into 5 groups is 7kg, 9kg, 10kg, 12kg and 15kg, the seed quantity of each group of ryegrass is 9, 11, 13, 14 and 15 grains, and after 7 days, the germination rate is counted and the medium-grade soil matrix is obtained after the field planting of three period times of plant growth.
6) Plants such as festuca arundinacea, bluegrass, alfalfa, and radix et rhizoma zingiberis are planted in the medium-class soil matrix. Sowing 13 seeds and 3 hundred vein root seeds, applying compound fertilizer once every three days, and calculating the germination rate after 7 days. After 3-period planting, the high-grade soil-like matrix is obtained.
Table 3 shows physical and chemical indexes of the soil after the improvement of the present example
| Project | Phosphogypsum group | Primary soil-like matrix | Medium class soil matrix | Advanced soil-like matrix |
| pH value of | 1.4 | 4.53 | 5.46 | 7.88 |
| Soil Total porosity (%) | / | 36％ | 43％ | 56％ |
| Nitrogen (%) | / | 0.09％ | 0.23％ | 0.32％ |
| Whole phosphorus (g/kg) | / | 0.43 | 0.55 | 0.77 |
| Whole potassium (g/kg) | / | 13 | 16 | 24 |
| Humic acid | / | 0.64％ | 0.21％ | 2.07％ |
From the data in table 3, it can be seen that the nutrient components in the primary soil-like matrix, the intermediate soil-like matrix and the high soil-like matrix are significantly improved, and the nutrient components of the soil in the primary soil-like matrix, the intermediate soil-like matrix and the high soil-like matrix are sequentially improved.
The phosphogypsum storage yard is converted into a soil-like matrix by combining the agricultural waste straws, so that the problem of phosphogypsum and the storage of the agricultural waste straws can be solved, and the soil problem can be improved.
The beneficial effects of the invention are as follows:
1. phosphogypsum comes from phosphogypsum yards, the method can solve the problem of piling up a large amount of phosphogypsum, and the method fully utilizes waste biomass and can also solve the problem of piling up a large amount of agricultural waste straws;
2. the phosphogypsum is combined with the waste biomass to carry out soil improvement, so that the pollution problem is solved, the better comprehensive waste pollution resources are realized, and the phosphogypsum has high application value and accords with the national concept of environmental protection and ecology;
3. the invention has the characteristics of simple process, high treatment efficiency, green ecology, low cost and the like.
The agriculture used by the invention can not only provide available nutrient substances for microorganisms after the waste straws are decomposed by cellulolytic bacteria, but also facilitate the field planting of the microorganisms in the straws after the microorganisms grow due to the structural characteristics of the agriculture. The phosphogypsum used by the invention not only can improve the soil environment, but also can increase the absorbable amount of phosphorus element in the middle of the action of microorganisms, enhance the fertility of soil and provide nutrients required by plants, and the phosphogypsum can improve the physicochemical environment of the soil, so that the phosphogypsum is improved into soil.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Furthermore, it should be understood that although the present disclosure describes embodiments, not every embodiment is provided with a separate embodiment, and that this description is provided for clarity only, and that the disclosure is not limited to the embodiments described in detail below, and that the embodiments described in the examples may be combined as appropriate to form other embodiments that will be apparent to those skilled in the art.
The foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and scope of the invention are intended to be included within the scope of the invention.
Claims (8)
1. The method for improving the phosphogypsum storage yard soil by utilizing the waste straws is characterized by comprising the following steps of:
s1: mixing phosphogypsum powder and alkaline soil according to a certain mass ratio to obtain pretreated phosphogypsum, wherein the preparation method comprises the following steps:
s01: the different separated and purified strains are respectively enriched and cultured in a liquid culture medium to obtain enriched culture solutions of different cellulose degrading bacteria, and the enriched culture solutions are specifically as follows:
s011: collecting saprophyte and surrounding soil samples, filling the saprophyte and the surrounding soil samples into a container according to a mass ratio (1-2) of 1 with sterile water, mixing and precipitating to obtain supernatant;
s012: filling the supernatant and the enrichment medium into a container according to the volume ratio (0.1-0.4): 1, and placing the container into a constant temperature shaking table at 25-35 ℃ for shaking culture at the rotating speed of 160-200 rpm to obtain an enrichment bacterial liquid;
s013: uniformly mixing the enriched bacterial liquid and a cellulolytic culture medium according to the volume ratio of (5-10), and culturing for 3-7 days at a constant temperature shaking table of 26-32 ℃ and a rotating speed of 160-200 rpm to obtain a cellulose composite bacterial liquid;
s014: inoculating the cellulose composite bacterial liquid into a cellulolytic culture medium, inversely culturing for 3-5 days at 26-32 ℃ to obtain bacterial colonies of cellulose degrading bacteria, selecting single bacterial colonies with different forms, purifying and culturing for 3-5 days at 26-32 ℃ by using a plate drawing line separation culture mode to obtain purified cellulolytic bacterial strains;
s015: inoculating the purified cellulose degrading strain into a cellulose degrading culture medium, and reversely culturing for 3-5 days at 28-30 ℃ to screen the cellulose degrading strain with transparent rings;
s016: inoculating the screened cellulose degradation strain into a liquid enzyme production culture medium, placing the liquid enzyme production culture medium in a constant temperature shaking table at 26-32 ℃ for shaking culture at a rotating speed of 160-200 rpm for 5-7 days, and comparing the total cellulase activity in the culture solution to obtain high-efficiency cellulose degradation bacteria;
s017: separating and screening strains of cermets, streptomyces, bacillus subtilis, trichoderma reesei, chaetomium and bacillus amyloliquefaciens, and placing the strains screened by the expansion culture medium into a constant temperature shaking table at 26-32 ℃ for culturing for 5-7 days at a rotating speed of 180-220 rpm to obtain different enrichment culture solutions after expansion culture;
s02: respectively carrying out generation domestication culture on the different enrichment culture solutions to obtain domestication culture solutions of different cellulose degrading bacteria, and mixing the different domestication culture solutions according to a certain volume ratio to obtain a composite bacterial solution
S2: mixing the pretreated phosphogypsum with straw powder, and spraying a composite bacterial liquid to obtain a primary soil-like matrix;
s3: introducing pioneer plants into the primary soil-like matrix to obtain a medium-grade soil-like matrix;
s4: and introducing common plants into the medium soil-like matrix to obtain the high soil-like matrix.
2. The method for improving phosphogypsum yard soil by using waste straw as claimed in claim 1, wherein the step S02 specifically comprises the following steps:
s021: inoculating the enriched culture solution of different cellulose degrading bacteria into domestication culture medium containing straw powder and phosphogypsum, and subculturing at 26-32deg.C for 4-7 times until fiber in each bacterial solutionThe total concentration of the vitamin degrading bacteria is 10 7 -10 9 Obtaining different cellulose degrading bacteria liquid by the method per mL;
s022: and (3) respectively carrying out expansion culture on different cellulose degrading bacterial solutions, and mixing the bacterial solutions according to a certain volume ratio to prepare the composite bacterial solution.
3. The method for improving phosphogypsum yard soil by using waste straws according to any one of claims 1-2, which is characterized in that: in the step S1, phosphogypsum and alkaline soil are mixed according to the mass ratio of 1 (2-4).
4. The method for improving the phosphogypsum yard soil by utilizing waste straws according to any one of claims 1-2, wherein the step S2 specifically comprises the following steps:
s21: the preparation method comprises the following steps of (1) pre-treating phosphogypsum and waste straw powder according to a mass ratio of 1: (1-3) placing the mixture into a stirrer, and mixing the mixture for 2-10 hours at a rotating speed of 40-60 revolutions per minute to obtain the organic phosphogypsum;
s22: the compound bacterial liquid is treated by 7-15L/(h.m) 2 ) The spraying speed of the water-based composite material is intermittently sprayed in the organic phosphogypsum for 1h at intervals of 1-2h, and the primary soil-like matrix is obtained after 15-60 days of growth and field planting.
5. The method for improving the phosphogypsum yard soil by utilizing waste straws according to any one of claims 1-2, wherein the step S3 specifically comprises the following steps:
and (2) planting pioneer plants in the primary soil-like matrix in the step (S2), and obtaining the intermediate soil-like matrix after 1-5 rounds of field planting growth.
6. The method for improving phosphogypsum yard soil by using waste straws as set forth in claim 5, which is characterized in that: in the step S3, the pioneer plant comprises one or more of pteris multifida and ryegrass.
7. The method for improving the phosphogypsum yard soil by utilizing waste straws according to any one of claims 1-2, wherein the step S4 specifically comprises the following steps: and (3) planting the common plants in the medium-class soil matrix in the step (S3), and obtaining the high-class soil matrix after 1-5 rounds of field planting growth.
8. The method for improving phosphogypsum yard soil by using waste straw as set forth in claim 7, which is characterized in that: in the step S4, the common plants comprise one or more of the group consisting of fescue, bluegrass, alfalfa and centella asiatica.
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