AU2021106648A4 - A method for formulating vegetables and fruits waste culture medium for industrial fungi cultivation - Google Patents

Abstract

A METHOD FOR FORMULATING VEGETABLES AND FRUITS WASTE CULTURE MEDIUM FOR INDUSTRIAL FUNGI CULTIVATION The present disclosure relates to a method for formulating vegetables and fruits waste culture medium for industrial fungi cultivation. In an aspect, the method (100) for formulating vegetables and fruits waste culture medium for industrial fungi cultivation comprises steps of collecting fruits and vegetable peels and preparing a fungal medium (102), preparing an inoculum (104) for platting of the industrially beneficial fungi in the fruits and vegetable peels waste medium prepared, addition (106) of the prepared medium and agar-agar in a particular ratio into a specific amount of distilled water, sterilizing (108) the mixture formed (106) by autoclaving at 121°C, addition (110) of prepared inoculum (104) to the cooled sterilized mixture (108), incubating (112) the medium (110) for the cultivation of industrially beneficial fungi. (Fig. 1 will be the reference figure) - 12- rage 1 01 1 100 Collecting fruits and vegetable peels and preparing a fungal medium. 102 Preparing an inoculum. 104 Addition of the prepared medium and agar-agar into distilled water. 106 Sterilizing the mixture. 108 Addition of the prepared inoculum to the cooled sterilized mixture. 110 Incubating the medium at room temperature and for about five days. 112 Fig. 1 Flowchart of a method for formulating vegetables and fruits waste culture medium for industrial fungi cultivation. 1

Description

rage 1 01 1

100

Collecting fruits and vegetable peels and preparing a fungal medium. 102

Preparing an inoculum. 104

Addition of the prepared medium and agar-agar into distilled water. 106

Sterilizing the mixture. 108

Addition of the prepared inoculum to the cooled sterilized mixture. 110

Incubating the medium at room temperature and for about five days. 112

Fig. 1 Flowchart of a method for formulating vegetables and fruits waste culture medium for industrial fungi cultivation.

A METHOD FOR FORMULATING VEGETABLES AND FRUITS WASTE CULTURE MEDIUM FOR INDUSTRIAL FUNGI CULTIVATION TECHNICAL FIELD

[0001] The present disclosure relates to a and in particular to a method for formulating vegetables and fruits waste culture medium for industrial fungi cultivation.

BACKGROUND

[0002] Background description includes information that may be useful in understanding the present invention. It is not an admission that any of the information provided herein is prior art or relevant to the presently claimed invention, or that any publication specifically or implicitly referenced is prior art.

[0003] India is an agriculture-based country, and the farming system has had its unique place in the universe from ancient days. Among the various agricultural products produced in India, vegetable cultivation has occupied a significant role due to its daily requirement for cooking purposes and multiple nutrients. India ranked second in the production of vegetables and marching towards the first rank because of the highest need due to the increase in population. Besides its beneficial role as a nutritive source in the food, the peel wastes generated from the vegetable create a nuisance to the environment. The management of wastes has been considered as a hot topic in various debates during recent times. The wastes generated from unspoiled vegetables are not harmful to human health and the environment, but it is essential to find a way for utilizing the vegetable peel wastes in a beneficial way. The disposal of unwanted agricultural wastes in a beneficial way is very challenging in the present scenario.

[0004] Efforts have been made in the related prior art to provide different solutions for fungi cultivation from organic waste. For example, a Chinese patent no. CN 100998294 B discloses method of utilizing method for culturing edible fungi using waste tree branches from garden during greening period, it is characterized in that: the discarded branch after the afforestation is dried, pulverizes the back become composts or fertilisers of cultivating mushroom with other mixed raw material, to divide after the composts or fertilisers of cultivating sterilization treatment in the culture vessel of packing into, to cultivate in the rare edible fungus species access culture vessel, obtain rare edible mushroom mycelium and fruit body, described composts or fertilisers of cultivating prescription is: needed raw material is in every 100ml composts or fertilisers of cultivating: potato 20g, discarded branch 10g, glucose 2g, potassium dihydrogen phosphate 0.3g, magnesium sulfate 0.15g, VB10.01g, dusty yeast 0.15g, agar 2g; The preparation method of described composts or fertilisers of cultivating is: potato is cleaned, peeling, stripping and slicing, weighs, mixes with branch, be soaked in water 1-2 hour, heat and boil 30-40min, filter and remove residue mixes filtrate with other raw material, stir, and is settled to volume required; The discarded seeds of described greening are a kind of in mountain ebony, banyan, mango or the yulan magnolia.

[0005] Therefore, the present disclosure overcomes the above-mentioned problem associated with the traditionally available method or system, any of the above-mentioned inventions can be used with the presented disclosed technique with or without modification.

[0006] All publications herein are incorporated by reference to the same extent as if each individual publication or patent application were specifically and individually indicated to be incorporated by reference. Where a definition or use of a term in an incorporated reference is inconsistent or contrary to the definition of that term provided herein, the definition of that term provided herein applies and the definition of that term in the reference does not apply.

[00071 In some embodiments, the numbers expressing quantities or dimensions of items, and so forth, used to describe and claim certain embodiments of the invention are to be understood as being modified in some instances by the term "about." Accordingly, in some embodiments, the numerical parameters set forth in the written description and attached claims are approximations that can vary depending upon the desired properties sought to be obtained by a particular embodiment. In some embodiments, the numerical parameters should be construed in light of the number of reported significant digits and by applying ordinary rounding techniques. Notwithstanding that the numerical ranges and parameters setting forth the broad scope of some embodiments of the invention are approximations, the numerical values set forth in the specific examples are reported as precisely as practicable. The numerical values presented in some embodiments of the invention may contain certain errors necessarily resulting from the standard deviation found in their respective testing measurements.

OBJECTS OF THE INVENTION

[0008] It is an object of the present disclosure which provides a method for formulating vegetables and fruits waste culture medium for industrial fungi cultivation.

SUMMARY

[0009] The present concept of the present invention is directed towards a method for formulating vegetables and fruits waste culture medium for industrial fungi cultivation.

[0010] In an aspect, the method for formulating vegetables and fruits waste culture medium for industrial fungi cultivation comprises steps of collecting fruits and vegetable peels and preparing a fungal medium, preparing an inoculum for platting of the industrially beneficial fungi in the fruits and vegetable peels waste medium prepared, addition of the prepared medium and agar-agar in a particular ratio into a specific amount of distilled water, sterilizing the mixture formed by autoclaving at 121°C for a specific duration of time depending upon the amount of mixture, addition of a small volume of prepared inoculum to the cooled sterilized mixture for initiation of growth of industrially beneficial fungi, incubating the medium at room temperature and for about five days for the cultivation of industrially beneficial fungi.

[0011] In another aspect, collecting a mixture of orange peel waste and garlic peel waste, among all other fruits and vegetable peels waste may result in the best cultivation of industrial fungi. Further, preparing a fungal medium from the collected vegetables and fruits peel waste may comprise steps of drying up of collected vegetables and fruits peel waste, powdering the dried peels waste with the help of a mixing machine, packing up the powdered mixture in a plastic cover, storing the packed mixture at room temperature.

[0012] In yet another aspect, preparing inoculum may comprise steps of preparing of suspension of three days old cultures of the industrially beneficial fungi in a saline solution of 0.85 % sodium chloride, inoculating the fungal cultures into a saline solution, incubating the culture at room temperature for 5 hours. The culture of industrially beneficial fungi comprises Aspergillus niger and Rhizopus stolonifera.

[0013] One should appreciate that although the present disclosure has been explained with respect to a defined set of functional modules, any other module or set of modules can be added/deleted/modified/combined, and any such changes in architecture/construction of the proposed system are completely within the scope of the present disclosure. Each module can also be fragmented into one or more functional sub-modules, all of which also completely within the scope of the present disclosure.

[0014] Various objects, features, aspects, and advantages of the inventive subject matter will become more apparent from the following detailed description of preferred embodiments, along with the accompanying drawing figures in which like numerals represent like components.

BRIEF DESCRIPTION OF THE DRAWINGS

[0015] The accompanying drawings are included to provide a further understanding of the present disclosure and are incorporated in and constitute a part of this specification. The drawings illustrate exemplary embodiments of the present disclosure and, together with the description, serve to explain the principles of the present disclosure.

[0016] Fig. 1 illustrates an exemplary flow chart of the method for formulating vegetables and fruits waste culture medium for industrial fungi cultivation.

[00171 It should be noted that the figures are not drawn to scale, and the elements of similar structure and functions are generally represented by like reference numerals for illustrative purposes throughout the figures. It should be noted that the figures do not illustrate every aspect of the described embodiments and do not limit the scope of the present disclosure.

[00181 Other objects, advantages, and novel features of the invention will become apparent from the following detailed description of the present embodiment when taken in conjunction with the accompanying drawings.

DETAILED DESCRIPTION

[0019] In the following description, numerous specific details are set forth in order to provide a thorough understanding of embodiments of the present invention. It will be apparent to one skilled in the art that embodiments of the present invention may be practised without some of these specific details.

[0020] Embodiments of the present invention include various steps, which will be described below. The steps may be performed by hardware components or may be embodied in machine-executable instructions, which may be used to cause a general-purpose or special purpose processor programmed with the instructions to perform the steps. Alternatively, steps may be performed by a combination of hardware, software, and firmware and/or by human operators.

[0021] If the specification states a component or feature "may", "can", "could", or "might" be included or have a characteristic, that particular component or feature is not required to be included or have the characteristic.

[0022] Exemplary embodiments will now be described more fully hereinafter with reference to the accompanying drawings, in which exemplary embodiments are shown. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. These embodiments are provided so that this disclosure will be thorough and complete and will fully convey the scope of the invention to those of ordinary skill in the art. Moreover, all statements herein reciting embodiments of the invention, as well as specific examples thereof, are intended to encompass both structural and functional equivalents thereof. Additionally, it is intended that such equivalents include both currently known equivalents as well as equivalents developed in the future (i.e., any elements developed that perform the same function, regardless of structure).

[0023] Thus, for example, it will be appreciated by those of ordinary skill in the art that the diagrams, schematics, illustrations, and the like represent conceptual views or processes illustrating systems and methods embodying this invention. The functions of the various elements shown in the figures may be provided through the use of dedicated hardware as well as hardware capable of executing associated software. Similarly, any switches shown in the figures are conceptual only. Their function may be carried out through the operation of program logic, through dedicated logic, through the interaction of program control and dedicated logic, or even manually, the particular technique being selectable by the entity implementing this invention. Those of ordinary skill in the art further understand that the exemplary hardware, software, processes, methods, and/or operating systems described herein are for illustrative purposes and, thus, are not intended to be limited to any particular named

[0024] The following is a detailed description of embodiments of the disclosure depicted in the accompanying drawings. The embodiments are in such detail as to clearly communicate the disclosure. However, the amount of detail offered is not intended to limit the anticipated variations of embodiments; on the contrary, the intention is to cover all modifications, equivalents, and alternatives falling within the spirit and scope of the present disclosure as defined by the appended claims.

[0025] Each of the appended claims defines a separate invention, which for infringement purposes is recognized as including equivalents to the various elements or limitations specified in the claims. Depending on the context, all references below to the "invention" may in some cases refer to certain specific embodiments only. In other cases, it will be recognized that references to the "invention" will refer to subject matter recited in one or more, but not necessarily all, of the claims.

[00261 Various terms as used herein are shown below. To the extent a term used in a claim is not defined below, it should be given the broadest definition persons in the pertinent art have given that term as reflected in printed publications and issued patents at the time of filing.

[00271 In an embodiment of the present disclosure, Fig. 1 illustrates an exemplary flow chart of the method for formulating vegetables and fruits waste culture medium for industrial fungi cultivation.

[0028] In an aspect, the method (100) for formulating vegetables and fruits waste culture medium for industrial fungi cultivation, wherein said method (100) comprises steps of collecting fruits and vegetable peels and preparing a fungal medium (102), preparing an inoculum (104) for platting of the industrially beneficial fungi in the fruits and vegetable peels waste medium prepared, addition (106) of the prepared medium and agar-agar in a particular ratio into a specific amount of distilled water, sterilizing (108) the mixture formed (106) by autoclaving at 121°C for a specific duration of time depending upon the amount of mixture, addition (110) of a small volume of prepared inoculum (104) to the cooled sterilized mixture (108) for initiation of growth of industrially beneficial fungi, incubating (112) the medium (110) at room temperature and for about five days for the cultivation of industrially beneficial fungi.

[0029] In an aspect, Microorganisms are the invisible creates which are ubiquitous in nature and present everywhere in this universe. The major categories of microorganisms in this universe are bacteria, archaea, fungi, algae, and protozoa. Among all microbial types, fungi take part an imperative role in the productions of various industrial products which are repeatedly used by human beings in their routine life. Fungi are the ubiquitous eukaryotes present in the air and soil. Based on their cellular nature, fungi are classified into two major groups, viz., Molds and Yeast. The molds are multicellular, and the yeast is unicellular in nature. The mold is widely spread in the air and soil in the form of spores, and the presence of yeast is very less in an environment.

[00301 In another aspect, collecting (102) a mixture of orange peel waste and garlic peel waste, among all other fruits and vegetable peels waste, results in the best cultivation of industrial fungi. Other than orange peel waste and garlic peel waste, plantain flower peel waste and/or pineapple peel waste could also be used in the form of a mixture of two or more substances or individually.

[0031] In another aspect, preparing (102) a fungal medium from the collected vegetables and fruits peel waste comprises steps of drying up of collected vegetables and fruits peels waste, powdering the dried peels waste with the help of a mixing machine, packing up the powdered mixture in a plastic cover, storing the packed mixture at room temperature.

[0032] In this aspect, the culture medium is an environment that favors the growth of microorganisms by providing various chemical nutrients and growth factors. Based on its properties, the culture medium is classified into six types viz., Basal medium, Enriched medium, Enrichment medium, Differential medium, Selective medium, and Transport medium. The nature of the culture medium was liquid, semi-solid, or solid, and technically the solid medium is referred to as the agar medium, and the liquid medium is called broth.

[0033] In another aspect, an autoclave is used in medical and laboratory settings to sterilize lab equipment and waste. Autoclave sterilization works by using heat to kill microorganisms such as bacteria and spores. The heat is delivered by pressurized steam. Pressurization allows the steam to reach the high temperatures that are required for sterilization. According to the Centers for Disease Control (CDC) Guidelines for Disinfection and Sterilization of Healthcare Facilities, pressurized steam is the most widely used and dependable method of sterilization. It's non-toxic and inexpensive, it kills microbes and spores rapidly, and it quickly heats and penetrates fabrics.

[0034] In yet another aspect, preparing inoculum (104) comprises steps of preparing of suspension of three days old cultures of the industrially beneficial fungi in a saline solution of 0.85 % sodium chloride, inoculating the fungal cultures into a saline solution, incubating the culture at room temperature for 5 hours. An incubator is a device used to grow and maintain microbiological cultures or cell cultures. The incubator maintains optimal temperature, humidity, and other conditions such as the C02 and oxygen content of the atmosphere inside. Incubators are essential for much experimental work in cell biology, microbiology, and molecular biology and are used to culture both bacterial and eukaryotic cells. The culture of industrially beneficial fungi comprises Aspergillus niger and Rhizopus stolonifera.

[0035] In yet another aspect, Aspergillus niger is one of the most important microorganisms used in biotechnology. It has been in use already for many decades to produce extracellular (food) enzymes and citric acid. In fact, citric acid and many A. niger enzymes are considered GRAS by the United States Food and Drug Administration. Rhizopus stolonifer has some importance to the environment. This specific type of mold plays a key role in the carbon cycle because it works as a decomposer in the soil, dung, and many food types. It is able to recycle materials, like sugar and starch, in order to make use of what has previously been used.

[0036] While the foregoing describes various embodiments of the invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof. The scope of the invention is determined by the claims that follow. The invention is not limited to the described embodiments, versions or examples, which are included to enable a person having ordinary skill in the art to make and use the invention when combined with information and knowledge available to the person having ordinary skill in the art.

[00371 Thus, the scope of the present disclosure is defined by the appended claims and includes both combinations and sub-combinations of the various features described hereinabove as well as variations and modifications thereof, which would occur to persons skilled in the art upon reading the foregoing description.

Claims (5)

I/We Claim:

1. A method (100) for formulating vegetables and fruits waste culture medium for industrial fungi cultivation, wherein said method (100) comprises steps of:

collecting fruits and vegetable peels and preparing a fungal medium (102);

preparing an inoculum (104), for platting of the industrially beneficial fungi in the fruits and vegetable peels waste medium prepared;

addition (106) of the prepared medium and agar-agar in a particular ratio into a specific amount of distilled water;

sterilizing (108) the mixture formed (106), by autoclaving at 121°C for a specific duration of time depending upon the amount of mixture;

addition (110) of a small volume of prepared inoculum (104) to the cooled sterilized mixture (108), for initiation of growth of industrially beneficial fungi;

incubating (112) the medium (110) at room temperature and for about five days for the cultivation of industrially beneficial fungi.

2. The method (100) for formulating vegetables and fruits waste culture medium for industrial fungi cultivation as claimed in claim 1, wherein collecting (102) a mixture of orange peel waste and garlic peel waste, among all other fruits and vegetable peels waste, results in the best cultivation of industrial fungi.

3. The method (100) for formulating vegetables and fruits waste culture medium for industrial fungi cultivation as claimed in claim 1, wherein preparing (102) a fungal medium from the collected vegetables and fruits peel waste comprises steps of:

drying up of collected vegetables and fruits peels waste;

powdering the dried peels waste with the help of a mixing machine;

packing up the powdered mixture in a plastic cover;

storing the packed mixture at room temperature.

4. The method (100) for formulating vegetables and fruits waste culture medium for industrial fungi cultivation as claimed in claim 1, wherein preparing inoculum (104) comprises steps of:

preparing of suspension of three days old cultures of the industrially beneficial fungi in a saline solution of 0.85 % sodium chloride;

inoculating the fungal cultures into a saline solution;

incubating the culture at room temperature for 5 hours.

5. The method (100) for formulating vegetables and fruits waste culture medium for industrial fungi cultivation as claimed in claims 1 and 4, wherein the culture of industrially beneficial fungi comprises Aspergillus niger and Rhizopus stolonifera.

Total no. of sheets: 1 23 Aug 2021 2021106648 Page 1 of 1

Fig. 1 Flowchart of a method for formulating vegetables and fruits waste culture medium for industrial fungi cultivation.