CN101811781B - White rot fungus rot agent and method for producing organic pollutant rot agent by using white rot fungi - Google Patents
White rot fungus rot agent and method for producing organic pollutant rot agent by using white rot fungi Download PDFInfo
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- CN101811781B CN101811781B CN2010101173565A CN201010117356A CN101811781B CN 101811781 B CN101811781 B CN 101811781B CN 2010101173565 A CN2010101173565 A CN 2010101173565A CN 201010117356 A CN201010117356 A CN 201010117356A CN 101811781 B CN101811781 B CN 101811781B
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Abstract
The invention provides a white rot fungus rot agent and a method for producing an organic pollutant rot agent by using white rot fungi. In the method, the white rot fungi are obtained in a large quantity through industrial production and used as a treating agent for waste water, sewages and excrements of paper mills, textile mills, printing and dyeing mills, food factories, livestock and poultry factories, and the like, can also be used as a quick rot agent of composts prepared from farmland straws applied to farmlands and straw pollutions and has higher economic values and good application prospects. The invention also provides an application of the white rot fungi used as a waste water treating agent. The production method of the white rot fungi comprises the following steps of: putting a culture medium in a culture container; after sterilization at a proper temperature, inoculating the white rot fungi to the culture medium and controlling the temperature, the humidity, the pressure and the pH condition of the culture medium; introducing sterile air; adsorbing by using attapulgite, peat, kaolin, and the like after culturing; drying by air or drying in the shade; and pulverizing to prepare into a solid or powdered finished product.
Description
Technical field:
The present invention relates to a kind of working method, and said preparation mainly refers to the application as paper mill, food plant wastewater treatment agent and livestock and poultry farm ight soil and sewage-treating agent and agricultural crop straw decomposition as the application as the environmental treatment agent with the whiterot fungi preparation.
Background technology:
Whiterot fungi (white rot fungus) is a kind of white filamentous fungus, and decay of wood is white in color.White-rot fungi belongs to Mycophyta on the taxonomy, is mainly Basidiomycetes, and minority is an ascomycetes, has rich diversity.For the decomposition of cellulose constituents; Whiterot fungi more is prone to lignin degrading; In decomposing rotten xylogen process, also decompose other polysaccharide simultaneously, can all be degraded to CO to the staple of timber, agricultural crop straw (xylogen, Mierocrystalline cellulose, semicellulose) under certain condition
2And H
2O accomplishes natural C, N biological cycle.
Whiterot fungi is natural to be present in the soil; Can secrete multiple enzyme; Be a kind of natural biological catalyzer treasure-house, indissoluble Mierocrystalline cellulose and xylogen in the plants such as catalytic hydrolysis timber become the small molecules dissolved organic matter, are the decomposer of occurring in nature insoluble organic and the driver of material cycle; The Natural Degradation mikrobe is a kind of ecological useful microbe.
There are several hundred tame paper mills in China; Several ten thousand tame food factories, textile mills, printing and dyeing mill, annual generation is also discharged tens tons the organic waste water that contains high density Mierocrystalline cellulose and xylogen, handles the discharging rivers as not adding; Must cause the rivers,lakes and seas environmental pollution; River COD and BOD severe overweight, mass consumption water oxygen gas causes hydrocoles mass mortality even extinction.
Simultaneously, there are the thousands of families of all kinds of livestock and poultry farm in China, the annual billions of tons of feces of livestock and poultry that produce, and except a part is applied to the farmland as organic fertilizer, in a lot of unprocessed discharging environment, final polluted-water causes algal tufa and fish kills, suffers heavy losses.
When the annual summer harvest and the autumn harvest, all produce a large amount of crop materials, be difficult to dispose, the peasant burns contaminate environment, influence aircraft flight, throws aside river, the obstruction river course, and deterioration water quality contains a large amount of xylogen in the paper mill effluent, cause serious environmental and water pollution.
Summary of the invention:
The object of the invention is to provide a kind of whiterot fungi to produce the method for organic pollutant decay agent, and this method is obtained the whiterot fungi preparation in a large number through suitability for industrialized production.
Another object of the present invention is the whiterot fungi decay agent.
The present invention also has a purpose to provide the application of whiterot fungi preparation, and it uses as High-Efficient Organic waste water, sewage-treating agent and feces of livestock and poultry and agricultural crop straw decomposition catalyzer, has very high economic worth and good prospects for application.
Whiterot fungi is produced the method for organic pollutant decay agent, it is characterized in that: at first solid medium is inserted in the culture vessel, after sterilization; The inoculation whiterot fungi is to substratum; Temperature, humidity, pressure and the pH condition of control substratum are cultivated, and cultivate the back and press culture weight and add 10-20% attapulgite, 20-50% peat and 10-20% kaolin and adsorb, and be air-dry or dry in the shade; Pulverize, make solid or Powdered finished product.
In the aforesaid method; The making of substratum: substratum is by weight percentage: 5-15% wheat bran, 5-20% rice chaff, 30-50% cotton seed hulls, 0.1-6% soybean cake powder, 0.3-5% flour, 0.2-2% ammonium sulfate, 0-0.5% potassium hydrogenphosphate, 0-0.3% sal epsom; Prepare with tap water; The control moisture content in medium is 40-50%, and control pH4.5-6.5 makes solid medium;
Place high-pressure sterilizing pot to carry out high-temperature sterilization 60-70 minute substratum, temperature is 121-125 ℃ in the maintenance Autoclave, and pressure is 0.12-0.2Mpa; Take out sterilized substratum, be placed on the indoor cooling of aseptic inoculation;
When substratum is cooled to 33 ℃ of left and right sides, the inoculation whiterot fungi, it is 1～8% that inoculum size accounts for the substratum weight percent, control culturing room temperature is 28-33 ℃, cultivates;
Stir substratum in the culturing process, after cultivating in 5～9 days, add above-mentioned attapulgite, peat and kaolin and mix thoroughly, adsorb, air-dry or dry in the shade, pulverize, make solid or Powdered finished product.
Substratum is stirred or stirred to this method in culturing process, stir or stir inferior 3-6 time every day in 1-2 days; Stir or stir 6-11 time every day in 3-6 days; Stir or stir 5-6 time every day in 7-9 days; Or substratum in the stirring cultivation pool.
A kind of whiterot fungi decay agent, its spy is: this decay agent is prepared from through following method: at first solid medium is inserted in the culture vessel, after sterilization; The inoculation whiterot fungi is to substratum, and temperature, pressure, the pH condition of control substratum are after the cultivation; Press culture weight and add 10-20% attapulgite, 20-50% peat and the absorption of 10-20% kaolin; Air-dry or dry in the shade, pulverize, make solid or Powdered finished product.
Above-mentioned whiterot fungi decay agent is in culturing process; Substratum is by weight percentage: 5-15% wheat bran, 5-20% rice chaff, 30-50% cotton seed hulls, 0.1-6% soybean cake powder, 0.3-5% flour, 0.2-2% ammonium sulfate, 0-0.5% potassium hydrogenphosphate, 0-0.3% sal epsom; Prepare with tap water; Control pH4.5-6.5 makes solid medium;
Place high-pressure sterilizing pot to carry out high-temperature sterilization 60-70 minute substratum, temperature is 121-125 ℃ in the maintenance Autoclave, and pressure is 0.12-0.2Mpa; Take out sterilized substratum, be placed on the indoor cooling of aseptic inoculation;
When substratum is cooled to 33 ℃ of left and right sides, the inoculation whiterot fungi, it is 1～8% that inoculum size accounts for the substratum weight percent, control culturing room temperature is 28-33 ℃, cultivates;
Stir substratum in the culturing process, after cultivating in 6～9 days, add above-mentioned attapulgite, peat, kaolin, mix thoroughly, adsorb, air-dry or dry in the shade, pulverize, make solid or Powdered finished product.
The application of whiterot fungi decay agent of the present invention aspect organic sewage, waste water, feces of livestock and poultry degraded and the farmland stalk decomposition in decomposition paper mill, food factory, textile mills, printing and dyeing mill's waste water and livestock and poultry farm, each place, pig farm.
The whiterot fungi preparation that the present invention produces is as the application of High-Efficient Organic wastewater and sewage and livestock and poultry manure treating agent and the quick decay agent of agricultural crop straw.
Utilizing high performance whiterot fungi preparation that the present invention obtains these unmanageable pollutents of catalyzed degradation rapidly, is that up-and-coming biotechnological formulation is arranged therefore.Can be used for handling the great amount of wastewater of paper mill, food factory, textile mills' discharging and a large amount of ight soil and the sewage of livestock and poultry farm discharging; And the discarded in a large number stalk in farmland; Unique effect is arranged; Low-consuming, effective, strongly professional, pollution less, does not injure natural enemy, environmental friendliness, therefore helps the human kind sustainable development and the eubiosis.
The present invention is a kind of working method of microbial preparation, utilizes whiterot fungi to produce viable bacteria and plurality of enzymes fermentoid thereof, through suitability for industrialized production, obtains whiterot fungi preparation efficiently in a large number.
The present invention inserts substratum in the culture vessel after sterilization, inoculates a certain amount of whiterot fungi, under certain temperature, humidity, pressure, nutrition, pH condition; Pass to sterile air; After for some time cultivates, add the suitable additive drying again, make solid or powder formulation product.
The whiterot fungi preparation that the present invention produces, production cost is low, and technology is simple, and is easy to operate, do not have pollution substance to be discharged in the environment in the production process.Utilize modern microbiological processes technology can be easy to realize indoor industrial, large-scale production; The easy artificial regulatory of working condition and production process; Quality is controlled easily, and product can carry out serialization, mass production, and special whiterot fungi preparation can reduce greatly to be produced and use cost; Improve product unit and tire, have very high economic worth and good prospects for application.
Compare the present invention with existing like product production technology and have following advantage:
The used working method of the present invention is easy, need not to carry out the quick-fried processing of vapour of fiber material, only needs to substitute with wood sawdust and rice chaff, saves the labour and the energy, and culture cycle only needs 4-6 days (solid culture).After the inoculation whiterot fungi cultivate to be accomplished to sterilising medium among the present invention, need not to extract various organized enzymes, only need that fermention medium and viable bacteria and various enzyme are pulverized drying with additive together and get final product saving labour, the energy, equipment input and production cost.The employed whiterot fungi of inoculation can be bought from relevant institution of higher learning such as Institute of Microorganism, Academia Sinica, DSMZ of the Chinese Academy of Agricultural Sciences, Shandong University, Wuhan University, China Agricultural University, scientific research institutions or scientific & technical corporation among the present invention.
Description of drawings:
Fig. 1 whiterot fungi is handled the paper mill effluent effect.
Fig. 2 whiterot fungi is handled the straw effect.
Embodiment:
Lift two embodiment at present, further specify.
Instance one: solid culture method
1, culture environment thorough disinfection comprises cultivation pool, koji tray, plastics bag, culturing room and cultivates room air.
2, with 5-20% wheat bran, 5-20% rice chaff, 30-50% cotton seed hulls, 0.5-6% soybean cake powder, 0.5-8% flour, 0.1-3% ammonium sulfate, 0-0.4% potassium hydrogenphosphate, 0-0.3% sal epsom; Add an amount of tap water; The adjustment water cut reaches about 40-50%; PH5-6.5 makes solid medium, and solid medium is placed cultivation pool, koji tray or plastics bag.
3, place high-pressure sterilizing pot to carry out high-temperature sterilization 60-70 minute packed above-mentioned raw materials, temperature is 121 ℃ in the maintenance Autoclave, and pressure is 0.15MPa.
4, take out sterilized packed substratum, be placed on the indoor cooling of aseptic inoculation, and placed 2-3 days.
5, will place 2-3 days the packed substratum of sterilization once more high-temperature sterilization once, method is killed the spore of surviving in sterilization process last time with 3, cooling.
6, when substratum in the bag is cooled to 33 ℃ of left and right sides, the inoculation whiterot fungi, the weight ratio that inoculum size accounts for substratum is 1～10%, control culturing room temperature is 28-32 ℃.Be placed on cultivation pool or the culturing rack of culturing room and cultivate, cultivate, after inoculation, plastics bag is evenly punched like plastics bag.
7, should repeatedly stir plastics bag or koji tray in the culturing process, stir inferior 3-5 time every day in 1-2 days; Stir 7-11 every day in 3-4 days; Stir 4-6 every day in 5-6 days; Or substratum in the stirring cultivation pool, so that substratum is evenly ventilative, make full use of culture material in order to the even growth of Fusarium oxysporum.
8, plate method adds 10-20% attapulgite, 20-50% peat, 10-20% kaolin etc. by culture weight and mixes thoroughly, adsorbs after cultivating in 6～9 days, and is air-dry or dry in the shade, and pulverizes, and makes solid or Powdered finished product.
Embodiment two:
1,15kg wheat bran, 15kg rice chaff, 45kg cotton seed hulls, 3kg soybean cake powder, 4.8kg flour, 2kg ammonium sulfate, 0.1kg potassium hydrogenphosphate, 0.1kg sal epsom etc. are added an amount of moisture content; The adjustment water cut reaches about 40-50%; PH5-6.5; Make solid medium, gross weight (moisture) 127.5kg places cultivation pool, koji tray or plastics bag with solid medium.
3, place high-pressure sterilizing pot to carry out high-temperature sterilization 60-70 minute packed above-mentioned raw materials, temperature is 121 ℃ in the maintenance Autoclave, and pressure is 0.15MPa.
4, take out sterilized packed substratum, be placed on the indoor cooling of aseptic inoculation, and placed 2-3 days.
5, will place 2-3 days the packed substratum of sterilization once more high-temperature sterilization once, method is killed the spore of surviving in sterilization process last time with 3, cooling.
6, when substratum in the bag is cooled to 33 ℃ of left and right sides, inoculation 8Kg whiterot fungi, control culturing room temperature is 28-32 ℃.Be placed on cultivation pool or the culturing rack of culturing room and cultivate, cultivate, after inoculation, plastics bag is evenly punched like plastics bag.
7, should repeatedly stir plastics bag or koji tray in the culturing process, stir inferior 3-5 time every day in 1-2 days; Stir 7-11 every day in 3-4 days; Stir 4-6 every day in 5-6 days; Or substratum in the stirring cultivation pool, so that substratum is evenly ventilative, make full use of culture material in order to the even growth of Fusarium oxysporum.
8, plate method is after cultivating in 6～9 days, culture 135.5Kg (weight in wet base), add 18kg attapulgite, 50kg peat, 15kg kaolin to culture and mix thoroughly, adsorb, air-dry or dry in the shade, pulverize solid or Powdered finished product 175.1kg.
Embodiment three:
The whiterot fungi product that the present invention is cultivated is as the papermaking sewage treatment agent; In the laboratory this product pulvis is added 25 gram whiterot fungi preparations by every liter of waste water; Start stir shaft stirring sewage and carry out the aeration cultivation, after 3 days, lignin degradation rate 64.5% above (see figure 1) in the sewage.
Embodiment four:
Product of the present invention is used as the agricultural crop straw decay agent, in the 28 ℃ of constant incubators in laboratory, add 10 gram whiterot fungi preparations by every kilogram of dry rice straw; Mix thoroughly, haystack water cut 50% was banked up 20 days; Haystack covers with white hypha, oven dry straw resistates, the whiterot fungi weight of formulation that deduction adds; Weighing straw residue weight reduces by 45.3% above (see figure 2) than weight before handling.
Claims (4)
1. whiterot fungi is produced the method for organic pollutant decay agent, it is characterized in that: at first solid medium is inserted in the culture vessel, after sterilization; The inoculation whiterot fungi is to substratum; Temperature, humidity, pressure and the pH condition of control substratum are cultivated, and cultivate the back and press culture weight and add 10-20% attapulgite, 20-50% peat and 10-20% kaolin and adsorb, and be air-dry or dry in the shade; Pulverize, make solid or Powdered finished product;
In the culturing process; Substratum is by weight percentage: 5-15% wheat bran, 5-20% rice chaff, 30-50% cotton seed hulls, 0.1-6% soybean cake powder, 0.3-5% flour, 0.2-2% ammonium sulfate, 0-0.5% potassium hydrogenphosphate, 0-0.3% sal epsom; Prepare with tap water; Control pH4.5-6.5 makes solid medium, and preparation back each composition total amount of substratum is 100%;
Place high-pressure sterilizing pot to carry out high-temperature sterilization 60-70 minute substratum, temperature is 121-125 ℃ in the maintenance Autoclave, and pressure is 0.12-0.2Mpa; Take out sterilized substratum, be placed on the indoor cooling of aseptic inoculation;
When substratum is cooled to 33 ℃, the inoculation whiterot fungi, it is 1～8% that inoculum size accounts for the substratum weight percent, control culturing room temperature is 28-33 ℃, cultivates;
Stir substratum in the culturing process, after cultivating in 5～9 days, add above-mentioned attapulgite, peat and kaolin and mix thoroughly, adsorb, air-dry or dry in the shade, pulverize, make solid or Powdered finished product.
2. according to the said method of producing the organic pollutant decay agent with whiterot fungi of claim 1, it is characterized in that: stir or stir substratum in the culturing process, stir or stir 3-6 time every day in 1-2 days; Stir or stir 6-11 time every day in 3-6 days; Stir or stir 5-6 time every day in 7-9 days; Or substratum in the stirring cultivation pool.
3. whiterot fungi decay agent, it is characterized in that: this decay agent is prepared from through following method: at first solid medium is inserted in the culture vessel, after sterilization; The inoculation whiterot fungi is to substratum, and temperature, pressure, the pH condition of control substratum are after the cultivation; Press culture weight and add 10-20% attapulgite, 20-50% peat and the absorption of 10-20% kaolin; Air-dry or dry in the shade, pulverize, make solid or Powdered finished product.
In the culturing process; Substratum is by weight percentage: 5-15% wheat bran, 5-20% rice chaff, 30-50% cotton seed hulls, 0.1-6% soybean cake powder, 0.3-5% flour, 0.2-2% ammonium sulfate, 0-0.5% potassium hydrogenphosphate, 0-0.3% sal epsom; Prepare with tap water; Control pH4.5-6.5 makes solid medium, and preparation back each composition total amount of substratum is 100%;
Place high-pressure sterilizing pot to carry out high-temperature sterilization 60-70 minute substratum, temperature is 121-125 ℃ in the maintenance Autoclave, and pressure is 0.12-0.2Mpa; Take out sterilized substratum, be placed on the indoor cooling of aseptic inoculation;
When substratum is cooled to 33 ℃, the inoculation whiterot fungi, it is 1～8% that inoculum size accounts for the substratum weight percent, control culturing room temperature is 28-33 ℃, cultivates;
Stir substratum in the culturing process, after cultivating in 6～9 days, add above-mentioned attapulgite, peat, kaolin, mix thoroughly, adsorb, air-dry or dry in the shade, pulverize, make solid or Powdered finished product.
4. the application of the said whiterot fungi decay agent of claim 3 aspect organic sewage, waste water, feces of livestock and poultry degraded and the farmland stalk decomposition in decomposition paper mill, food factory, textile mills, printing and dyeing mill's waste water and livestock and poultry farm, each place, pig farm.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2010101173565A CN101811781B (en) | 2010-03-04 | 2010-03-04 | White rot fungus rot agent and method for producing organic pollutant rot agent by using white rot fungi |
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| CN2010101173565A CN101811781B (en) | 2010-03-04 | 2010-03-04 | White rot fungus rot agent and method for producing organic pollutant rot agent by using white rot fungi |
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| CN101811781B true CN101811781B (en) | 2012-01-25 |
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| CN103951477B (en) * | 2014-03-28 | 2016-07-20 | 甘肃富民生态农业科技有限公司 | A kind of straw decomposing inoculant and production method thereof |
| CN105417726B (en) * | 2015-12-15 | 2018-03-30 | 安徽皖维高新材料股份有限公司 | A kind of method using white-rot fungi processing polyvinyl alcohol production waste water |
| CN111825218B (en) * | 2020-03-18 | 2022-03-22 | 广东省生态环境技术研究所 | Method for degrading triphenyl phosphate by using pycnoporus sanguineus and application of method |
| CN111807632B (en) * | 2020-07-21 | 2023-01-31 | 吉林化工学院 | Method for treating dye wastewater by using modified fly ash |
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| CN1421402A (en) * | 2001-11-30 | 2003-06-04 | 中国人民解放军军事医学科学院卫生学环境医学研究所 | Microbial prepn for treating paper-making effluent and microbe culturing method |
| CN1724656A (en) * | 2004-07-23 | 2006-01-25 | 中国科学院沈阳应用生态研究所 | A kind of preparation method who is used for the high vigor compound enzymic preparation of caragana microphylla degraded |
| JP2006314858A (en) * | 2005-05-10 | 2006-11-24 | Petroleum Energy Center | Method of purifying soil or water contaminated with heavy oil |
| CN101549936B (en) * | 2009-05-04 | 2011-06-29 | 清华大学 | A method using white-rot fungus and carrier to process persistent wastewater |
| CN101591620A (en) * | 2009-06-30 | 2009-12-02 | 重庆大学 | A kind of method of propagation culture and solid fermentation of white-rot fungi |
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