CN101549936B - A method using white-rot fungus and carrier to process persistent wastewater - Google Patents

Abstract

The invention is a method using white-rot fungus and carrier to process persistent wastewater which belongs to the field of water treatment technology. It first uses the carrier to absorb COD in wastewater, then uses the carrier to provide cellulose stimulating substrate to the white-rot fungus for its enzyme production, processes the persistent substance adsorbed on the carrier, selects differentnatural products as the core materials of the carrier, and produces the carrier coat after NaOH treatment. When handling the waste water, it adjusts the initial wastewater pH, adds the carrier and wh ite-rot fungi, and takes the water sample into the conical flask; places the sample in the shaking table with the temperature of 35-60 DEG C and shaking speed of 170rpm for processing. The treatment process of the invention only needs to adjust the initial pH, has no operation during the middle process and does not need re-adjustment for subsequent treatment; the carrier used with a wide variety of sources can be treated directly as the fertilizer together with the bacteria after use, and has no secondary pollution; it has apparent decolorization effect after processing with the decolorizationrate of above 90%.

Description

Utilize the method for white-rot fungi and carrier to process persistent wastewater

Technical field

The invention belongs to water-treatment technology field, particularly a kind of method of utilizing white-rot fungi and carrier to process persistent wastewater that high-concentration hardly-degradable waste water is handled.

Background technology

Along with the increase of Chinese economic development and social demand, quantity of wastewater effluent increases day by day, and wherein the trade effluent increment increases rapidly.Along with the increase of Chemicals demand, wastewater from chemical industry also increases thereupon in recent years.COD up to several ten thousand milligrams every liter is not only arranged in these water, and colourity height, some waste water contains the multiple material that the heavy metal ion of bio-toxicity is arranged and suppress microorganism growth, not only environment is caused very big pressure, but also has increased the difficulty with biological treatment.How to handle this class waste water, become a great difficult problem in the current environment protection.

White-rot fungi is organic sphere one a class filamentous fungus, and they are subjected to the world to unique degradation capability of various xenobionticses and attract attention.White-rot fungi is except have the very strong degradation capability wooden, to other as poisonous and hazardous organism such as DDT, chloro diphenyl, dioxin and phenol very strong degradation capability is arranged also, this ability depends on its extracellular lignin degradation enzyme system.White-rot fungi and related application technology thereof demonstrate powerful potentiality just day by day at field of environment protection.But all now test-results all are in laboratory level, can't be applied in the actual engineering and go.Because white-rot fungi belongs to eukaryote, the speed of growth wants slow many than bacterium, when in the reaction system bacterium being arranged, bacterium is breeding fast, nutritive ingredient with in the white-rot fungi competition substratum keep on top in whole reaction system soon, and white-rot fungi can stop growing in default of nutritive substance, and then influence the born of the same parents production and the secretion of degradation enzyme system outward, make the entire treatment system lose normal degradation capability.

Therefore, the superiority that only in reactive system, keeps white-rot fungi, and provide a stable carbon source to it, the eubolism and the growth conditions that just keep white rot fungus secretion extracellular enzyme system, be unlikely to enter in default of carbon source spore vegetative period, this also is the major cause that the restriction white-rot fungi applies to actual industrial.

Recognized all both at home and abroad that in recent years keeping the superiority of white-rot fungi in reactor is the key issue that the utilization white-rot fungi is handled waste water, a lot of researchs efforts be made so that all doing carrier with the material of bacteria growing inhibiting reaches this purpose, but this mode is difficult to keep the stable treated effect in actual use, and major part all needs to control nitrogenous source and just can achieve the goal.So not only can increase extra burden, and also be not easy to operation in actual applications.

Patent CN1608755A has adopted the two-part processing mode, the growth and the degradation period of white-rot fungi are separated, can keep the advantage of white-rot fungi in reactor like this, and can keep higher enzyme to live, though can reach treatment effect preferably, but the reactor load ability is not high, and the time is longer; Though solved the problem of mud-water separation, owing to can not handle continuously, floor space is bigger, is unfavorable for actual popularization.

Patent CN1793354A has adopted the inert support-polyurethane foam carrier with best fungistatic effect, the advantage that can keep white-rot fungi, and reach good decolorizing effect, but be to use this carrier after discarded, can become new difficult waste, and need to cooperate the special substratum that suppresses bacterium, the effect of using continuously in the open system is difficult to reach the stable treated effect, and for other more complicated trade effluents of composition whether the good treatment effect is arranged, and is still waiting to confirm.

Summary of the invention

The objective of the invention is to propose to solve white-rot fungi difficult cultivation in practice, active low a kind of method of utilizing white-rot fungi and carrier to process persistent wastewater, it is characterized in that, utilize the COD in the carrier absorption waste water earlier, after utilize carrier to provide Mierocrystalline cellulose pungency substrate to bring out it to produce enzyme, handle the hard-degraded substance of carrier absorption, and do not produce too much mycelium to white-rot fungi, pilot process is only regulated pH, and the facility of need not sterilizing, not limiting nitrogenous source, this method comprises:

1) bacterial classification is selected: the own Phanerochaete chrysosporium (CICC 40719) in laboratory (Phanerochaetechrysosporium) and from the variable color bolt bacterium (CICC 50001) of bacterial classification preservation center buying (Trametesversicolor).

2) liquid nutrient medium: potato leaches powder substratum and microzyme culture medium, and the former is the enrichment white-rot fungi in a large number, for environment bigger adaptability is arranged; The latter can cultivate the less mycelium pellet of particle diameter, has stronger degradability.Potato leaches the powder medium component: potato leaches powder 4g/100ml, glucose 2g/100ml, KH ₂PO ₄: 0.3g/100ml, MgSO ₄: 0.15g/100ml; The yeast culture based component is: glucose 0.5g/100ml, KH ₂PO ₄: 0.1g/100ml, (NH ₄) ₂SO ₄: 0.1g/100ml, MgSO ₄7H ₂O 0.05g/100ml, yeast extract paste: 0.02g/100ml, pH 5.0-6.0.Liquid substratum is put into the 250ml Erlenmeyer flask with 121 ℃ of sterilization 20min, inoculates white-rot fungi 0.05g after cooling, insert temperature 28-34 ℃ then, shake in the fast 170rpm shaking table, cultivated 3 days.

3) solid support material: selecting the core material of different natural goodses as carrier for use, is respectively maize straw and gas explosion maize straw, uses 3%NaOH soaking and washing 30min earlier, and clear water re-uses 3%H after cleaning ₂SO ₄Soaking and washing 30min, clear water clean to neutral back and use 75% industrial spirit to soak 30min, insert in 50 ℃ of baking ovens after filter is done and dry 5～6 hours, insert 121 ℃ of sterilization 15min after the oven dry, and be standby.

4) carrier bag quilt: each carrier contains natural goods 0.3g, and wraps up with copper mesh and wire netting in the core outside, and copper mesh 1.8 ± 0.2g, wire netting 1.1 ± 0.2g cover with nylon wire at last.

5) treating processes:

(1) handles water sample and be respectively the former water of Avrmectin, UASB (upflow anaerobic sludge blanket reactor) water outlet and biochemistry pool water outlet.

(2) the initial pH of adjusting waste water is 4-5.5, adds 3 carriers, 0.3-0.5g white-rot fungi, 100ml water sample in the 250ml Erlenmeyer flask; Insert temperature 35-39 ℃, shake fast 170rpm shaking table and handle.

(3) after wastewater treatment finishes, keep throw out, reactor changes water to be continued to handle.

The invention has the beneficial effects as follows that it is 4.5 that treating processes only need be regulated initial pH, pilot process need not be operated, and water outlet pH is about 6.5, need not to regulate once more to carry out subsequent disposal; And used carrier wide material sources can directly be made fertilizer together with thalline after the use and handle, and do not produce secondary pollution; It is obvious to handle the rear decoloring effect, percent of decolourization is more than 90%, though other assorted bacterium are arranged in the reactor, but the bulk treatment effect be there is no negative impact, for white-rot fungi provides a kind of possible treatment process in real project using, and reduced the cost of handling waste water, reduced the difficulty of operation.

Embodiment

The present invention proposes to solve white-rot fungi difficult cultivation in practice, active low a kind of method of utilizing white-rot fungi and carrier to process persistent wastewater.The present invention cooperates specific support to use for a kind of white-rot fungi that utilizes under non-sterilized environment, can fixed white rot fungi and supplementary carbon source matrix, reach and strengthen function, because the favourable absorption of selected carrier, this carrier also has good effect to the degraded of total phosphorus in the waste water.Enumerating embodiment is below further specified the present invention.

Embodiment 1: the best incorporated summary of the invention

Handle the waste water of high salinity, high phenol content: waste water is 120mg/L through extraction back phenol, and COD is about 2000, SO ₄ ^2-Concentration be about 100000mg/l.The initial pH that regulates waste water is 5, adds 3 carriers, 0.3g white-rot fungi, 100ml water sample in the 250ml Erlenmeyer flask; After inserting 38 ℃ of temperature, shaking fast 170rpm shaking table and handle pre-treatment in 48 hours, phenol content can be reduced to about 10mg/L, COD is reduced to below 600, and through active sludge treatment after 24 hours, COD can be reduced to about 200, phenol content is about 2mg/L, reaches emission standard through after the adsorption filtration again;

Embodiment 2

Handle high COD, the Avermectin Wastewater of high chroma:

The former water of Avrmectin: COD is 143.44mg/L up to 25000mg/L, colourity about 7691, T-P, and the initial pH that regulates waste water is 4.5, adds 3 carriers, 0.4g white-rot fungi, 100ml water sample in the 250ml Erlenmeyer flask; After inserting 37.5 ℃ of temperature, shaking fast 170rpm shaking table and handle 4 days, the minimum 4180mg/L that reduces to of COD, colourity can be reduced to 542, T-P can be reduced to 2mg/L;

Embodiment 3

Handling the UASB water outlet of Avrmectin: COD and be 1139.96mg/L, colourity 1743.82, T-P is 17.341mg/L, and the initial pH that regulates waste water is 5.5, adds 4 carriers, 0.4g white-rot fungi, 100ml water sample in the 250ml Erlenmeyer flask; Insert 36.5 ℃ of temperature, shake fast 170rpm shaking table and pass through present method and handle after 2 days, COD can reduce to that 294.76mg/L, colourity can reduce to 61.122, T-P reaches 0.2038mg/L;

Embodiment 4

The UASB water outlet of embodiment 3 is handled through biochemistry pool again: COD is 541.6mg/L, and the treatment process of process embodiment 3 was handled after 1 day, and COD can be reduced to 168.4mg/L.

Claims (1)

1. one kind cooperates specific support to use under non-sterilized environment, can fixed white rot fungi and strengthen its function, and processing method for waste water that can practical application in wastewater treatment is characterized in that this method comprises:

1) bacterial classification is selected: Phanerochaete chrysosporium (Phanerochaete chrysosporium) (CICC 40719) that the laboratory is own and the variable color bolt bacterium (Trametes versicolor) (CICC 50001) of purchasing from bacterial classification preservation center;

2) liquid nutrient medium: potato leaches powder substratum and microzyme culture medium, and the former a large amount of enrichment white-rot fungis have bigger adaptability for environment; The latter cultivates the less mycelium pellet of particle diameter, has stronger degradability; Potato leaches the powder medium component: potato leaches powder 4g/100ml, glucose 2g/100ml, KH ₂PO ₄: 0.3g/100ml, MgSO ₄: 0.15g/100ml; The microzyme culture medium composition is: glucose 0.5g/100ml, KH ₂PO ₄: 0.1g/100ml, (NH ₄) ₂SO ₄: 0.1g/100ml, MgSO ₄7H ₂O 0.05g/100ml, yeast extract paste: 0.02g/100ml, pH 5.0-6.0; Liquid substratum is put into the 250ml Erlenmeyer flask with 121 ℃ of sterilization 20min, inoculates white-rot fungi 0.05g after cooling, insert temperature 28-34 ℃ then, shake in the fast 170rpm shaking table, cultivated 3 days;

3) solid support material: selecting the core material of different natural goodses as carrier for use, is respectively maize straw and gas explosion maize straw, uses 3%NaOH soaking and washing 30min earlier, and clear water re-uses 3%H after cleaning ₂SO ₄Soaking and washing 30min, clear water clean after neutral and use 75% industrial spirit to soak 30min, insert in 50 ℃ of baking ovens after filter is done and dry 5-6 hour, insert 121 ℃ of sterilization 15min after the oven dry, and be standby;

4) carrier bag quilt: each carrier contains natural goods 0.3g, and wraps up with copper mesh and wire netting in the core outside, and copper mesh 1.8 ± 0.2g, wire netting 1.1 ± 0.2g cover with nylon wire at last;

5) treating processes:

(1) handle water sample and be respectively the former water of Avrmectin, UASB water outlet and biochemistry pool water outlet, wherein UASB is a upflow anaerobic sludge blanket reactor;

(2) the initial pH of adjusting waste water is 4-5.5, adds 3 carriers, 0.3-0.5g white-rot fungi, 100ml water sample in the 250ml Erlenmeyer flask; Insert temperature 35-60 ℃, shake fast 170rpm shaking table and handle;

(3) after wastewater treatment finishes, keep throw out, reactor changes water to be continued to handle.