CN103102015A - Method for treatment of organophosphorus pesticide wastewater by immobilized microorganisms - Google Patents
Method for treatment of organophosphorus pesticide wastewater by immobilized microorganisms Download PDFInfo
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- CN103102015A CN103102015A CN 201110351867 CN201110351867A CN103102015A CN 103102015 A CN103102015 A CN 103102015A CN 201110351867 CN201110351867 CN 201110351867 CN 201110351867 A CN201110351867 A CN 201110351867A CN 103102015 A CN103102015 A CN 103102015A
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- aspergillus niger
- organophosphorus pesticide
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- fungi aspergillus
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Abstract
The invention relates to a method for treatment of organophosphorus pesticide wastewater by immobilized microorganisms. Mainly composed of the processes of strain domestication, carrier preparation, bacterium adsorption and wastewater treatment, the method is characterized by: adsorbing domesticated fungus Aspergillus niger to activated carbon with a diameter of 5-8mml, then putting the prepared activated carbon containing saturated Aspergillus niger into wastewater, with the putting amount of activated carbon accounting for 1% of the wastewater when the CODcr (chemical oxygen demand) of each liter of sewage reaches 200-10000, controlling the temperature at 530DEG C, and performing static immersion for 1-6 days. By using the method to treat the organophosphorus pesticide wastewater, the degradation rate can reach up to 98%.
Description
Technical field
The present invention is a kind of improvement technology of utilizing microorganism efficiently degrading organophosphorus pesticides combined pollution waste water.
Background technology
Microbial immobilized technology is the important channel of the efficient effect of microorganism performance, especially in contaminate environment improvement field, become the core technology of the techniques such as the quick removal of some sewage disposal, disposition of solid waste and Organic aerosol, becoming the focus of current environment scientific and engineering research.The process for fixation of having reported is various, but there is no at present a kind of desirable, industrial method commonplace applicable organophosphorus pesticide wastewater.
Livingston etc. utilize microbial immobilized reactor for treatment to contain organophosphorus waste water, and the clearance of CODcr all reaches more than 86%; Ferschl etc. are take sodium alginate-calcium chloride solution as embedding system immobilization pseudomonas acidovorans (Pseudomonas acidovorus), the continuous degradation acephatemet, result is pointed out: after immobilization, it is good that the enzymic activity of microorganism secretion keeps, and in unit volume, concentration increases; Johnson etc. utilize immobilized microbiological deterioration acephatemet, and research is found: under aerobic condition, acephatemet is after 5 days, and degradation rate reaches 88%, has obtained repairing effect preferably.
Biotech development has become the Main Means of countries in the world Treating Municipal Sewage and organic waste water so far, has that processing power is large, automation degree of equipment is high, is easy to regulation and control, the characteristics such as economically feasible, non-secondary pollution.China is large agricultural country, is produced in a large number and uses for the good harvest agricultural chemicals that obtains agricultural, causes ecotope to be subject to havoc, and the environmental problem that is wherein caused with the organophosphorus pesticide factory effluent is the most serious.
The specific purposes of this research are will explore immobilized high efficiency biodegradation bacterium to the efficient improvement technology of organophosphorus pesticide combined pollution waste water.Screening, many high-efficiency degradations of domestication bacterium; Utilize the method for chemistry or physics, with free microorganism, in the area of space that is positioned to limit, and make it keep active, and can Reusability.Propose the combination process of the complete treatment based on immobilized microorganisms organophosphorus pesticide factory effluent of a cover, reduce the COD of wastewater effluent
crAnd TOC, with the agricultural chemicals waste water resource utilization.Result of study provides the technical director for promoting this technology in the rig-site utilization of clothing medicine manufacture waste water.
The character of organophosphorus pesticide factory effluent and source are different, all the pollution level to environment water is large, and intractability is high, administer difficulty, therefore, the investigator of this invention adopts immobilized high efficiency biodegradation bacterium that the organophosphorus pesticide combined pollution waste water is efficiently administered,, toxicity high in concentration reaches greatly under the condition of pollutant component complexity, investigate efficient degrading bacteria to the patience of environment with to the clearance of organophosphorus pesticide, and the conditions such as immobilization material, carrier concn, temperature and acidity of inquiring into are on the impact of organophosphorus pesticide factory effluent.Through repetition test, thereby reached comparatively desirable effect.
Summary of the invention
This project will be from multiple immobilization material a kind of through the experimental selection excellent property, adopt the microorganism immobilization method of the most suitable this kind material to test, administered the most afterwards the organophosphorus pesticide factory effluent.
(1) screening of microorganism domestication
When adopting microbial immobilized technology governance organophosphorus pesticide wastewater, the efficient degradation screening is crucial.Use ecological existing 10 fungal strains of the Chinese Academy of Sciences (verified can the lasting organic pollutant of efficient degradation polycyclic aromatic hydrocarbons) to screen domestication; At first use the organophosphorus pesticide methyl phosphorus of high density to tame, Pollutant levels adopt gradient type to strengthen gradually consumption, and the preliminary examination concentration of methyl phosphorus is 50ppm; The methyl phosphorus that adds again 50ppm after 3 days in this substratum, when the concentration of methyl phosphorus was 500ppm, domestication stopped. last, through conscientiously identifying, can enduring high-concentration methyl phosphorus and dominant bacteria that can this material of efficient degradation be the fungi aspergillus niger.
(2) selection of immobilization material
Compared the carrier immobilized effect of 10 kinds of differing materials in trial test, the results are shown in Table.
Table 1 fixation support relatively
This shows, take peat as carrier, its effect is relatively good with gac and No. 10 experiment in No. 4 experiments, and gac wherein is cheap, cost is low, wide material sources, and air permeability is good, nontoxic, can not cause the secondary pollution of soil, be fit to do the fixation support use of fungi aspergillus niger.
(3) selection of process for fixation
This project adopts gac as the fixation support material, utilizes 3 kinds of microorganism immobilization methods such as absorption method, crosslinking and entrapping method to test, and is wherein best by absorption method fixed black aspergillus effect.The gac that will contain aspergillus niger in test under aseptic condition joins in the substratum that the methyl phosphorus concentration is 150ppm, put constant-temperature table into, after cultivating 6 days with the revolution of 200r/min under room temperature, with the residual quantity of gas Chromatographic Determination methyl phosphorus, thereby calculate degradation rate.Experimental result shows, adopts absorption method fixed black aspergillus to be up to 98% to the degradation rate of target contaminant methyl phosphorus.
The front is said to be utilized absorption method that the fungi aspergillus niger is fixed to the way of taking on gac to be:
(1) domestication of fungi aspergillus niger uses the organophosphorus pesticide methyl phosphorus of high density to tame, and adopts gradient type to strengthen gradually consumption, at first adds methyl phosphorus in containing the potato substratum of fungi aspergillus niger, and it adds concentration to reach 50ppm; Cross the methyl phosphorus that adds again 50ppm after 3 days in this substratum, when the concentration of methyl phosphorus was 500ppm, domestication stopped.Obtain can potent degrading organic phosphor pesticides the fungi aspergillus niger.
(2) preparation of fungi aspergillus niger seed liquor
The potato-based nutrient solution 1000mL of the 121 ℃ of sterilising treatment of learning from else's experience, then getting a ring aspergillus niger at sterilisable chamber puts into wherein, put it into shaking table, stirring intensity is that 150-200r/min cultivated after 12-18 hour, obtains the approximately fungi aspergillus niger seed liquor of 40% left and right again.
(3) gac preparation is crushed to common gac below 100 orders, adds the common bond of quality of activated carbon 3%, artificial pelletizing then, and its particle size diameter is 5-8mm, drying;
(4) fungi absorption, to be joined in the reactor that spheric active carbon is housed by the fungi aspergillus niger 10-15% culture medium solution that contains that the seed liquor dilution makes, the ratio of fungi aspergillus niger culture medium solution and active carbon spheres quality is 1: 2, mix 6-8 hour in reactor at the temperature of 5-30 degree, stirring intensity is 150-200r/min, then filter being fixed mycelium pellet in sterilisable chamber, and in 0-4 degree temperature Refrigerator store.
When practical application, when the CODcr value in sewage 200---in the time of in 10000 scopes, put among waste water in the ratio of sewage quality 1-2% making its quality of gac of containing aspergillus niger, temperature is controlled at the 5-30 degree, the container that then above-mentioned waste water and mycelium pellet will be housed is put into constant-temperature table, stirring intensity is 150-200r/min, and the degradation rate that reaches CODcr after 3-6 days storage period can reach more than 99%.
And after using the method immobilization, fixation support can reach
1. can control immobilized cell particle size, porosity and perviousness;
2. fixing condition and process are gentle as far as possible;
3. fixation support is for the cell paste inertia;
4. carrier possesses good physical strength and chemical stability;
5. improve the intensity of immobilization particle;
Advantage and the effect of this invention are as follows:
Utilize that microbial immobilized improvement organophosphorus pesticide factory effluent has non-secondary pollution, cheap, good mechanical property, cheap, method is simple, the advantage such as quick.This invention has realized following concrete innovation
(1) exploitation low cost, high performance raw material carry out the immobilization correlative study of efficient degrading bacteria, application approach and market have been opened up for utilizing the low-cost resource of China to produce high value-added product, meet protection of the environment and resource-conserving nation-building, and solve the resource problem of China's water quality lack of water;
(2) will dissociate bacterium and immobilized microorganism administered organophosphorus waste water effect and compares experiment, seeks best microbial immobilized technique, and efficient degradation methyl phosphorus agricultural chemicals is easy to industrialization, and its method can form patent of invention;
(3) carry out comprehensive performance test for the immobilization product, obtain on the basis of the desirable over-all propertieies such as intensity, non-secondary pollution and stable performance at it, target is not limited to the improvement of organophosphorus pesticide wastewater, we aim at the concrete application of the improvement such as all organic industrial sewages aspect, can solve country is badly in need of, solve the environmental issue of global industrial water quality lack of water, the exploitation of the new purposes of this immobilization material is also a kind of innovation.
Embodiment
Screening, many high-efficiency degradations of domestication bacterium are wherein take the fungi aspergillus niger as best.Adopt 3 kinds of microorganism immobilization methods to test, determine that at last gac is immobilization material, and adopt absorption method fixed black aspergillus, administer the organophosphorus pesticide factory effluent with this, respond well.Obviously reduce the COD of wastewater effluent
crAnd TOC, with the agricultural chemicals waste water resource utilization.
Claims (1)
1. a way of utilizing immobilized microorganism to administer organophosphorus pesticide wastewater, mainly prepare through strain domestication, carrier, and the several processes of bacterial adsorption and sewage disposal is characterized in that:
(1) domestication of fungi aspergillus niger uses the organophosphorus pesticide methyl phosphorus of high density to tame, and adopts gradient type to strengthen gradually consumption, at first adds methyl phosphorus in containing the potato substratum of fungi aspergillus niger, and it adds concentration to reach 50ppm; Cross the methyl phosphorus that adds again 50ppm after 3 days in this substratum, when the concentration of methyl phosphorus was 500ppm, domestication stopped.Obtain can potent degrading organic phosphor pesticides the fungi aspergillus niger.
(2) preparation of fungi aspergillus niger seed liquor
The potato-based nutrient solution 1000mL of the 121 ℃ of sterilising treatment of learning from else's experience, then getting a ring aspergillus niger at sterilisable chamber puts into wherein, put it into shaking table, stirring intensity is that 150-200r/min cultivated after 12-18 hour, obtains the approximately fungi aspergillus niger seed liquor of 40% left and right again.
(3) gac preparation is crushed to common gac below 100 orders, adds the common bond of quality of activated carbon 3%, artificial pelletizing then, and its particle size diameter is 5-8mm, drying;
(4) fungi absorption, to be joined in the reactor that spheric active carbon is housed by the fungi aspergillus niger 10-15% culture medium solution that contains that the seed liquor dilution makes, the ratio of fungi aspergillus niger culture medium solution and active carbon spheres quality is 1: 2, mix 6-8 hour in reactor at the temperature of 5-30 degree, stirring intensity is 150-200r/min, then filter being fixed mycelium pellet in sterilisable chamber, and in 0-4 degree temperature Refrigerator store.
(5) when the CODcr value in sewage 200---in the time of in 10000 scopes, put among waste water in the ratio of sewage quality 1-2% making its quality of gac of containing aspergillus niger, temperature is controlled at the 5-30 degree, the container that then above-mentioned waste water and mycelium pellet will be housed is put into constant-temperature table, stirring intensity is 150-200r/min, and be 3-6 days storage period.
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| CN 201110351867 CN103102015A (en) | 2011-11-09 | 2011-11-09 | Method for treatment of organophosphorus pesticide wastewater by immobilized microorganisms |
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| CN 201110351867 CN103102015A (en) | 2011-11-09 | 2011-11-09 | Method for treatment of organophosphorus pesticide wastewater by immobilized microorganisms |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104671437A (en) * | 2015-03-03 | 2015-06-03 | 南华大学 | Method for remedying U (VI) polluted water body by decomposing ground phosphate rock with Aspergillus niger |
| CN105598158A (en) * | 2016-01-28 | 2016-05-25 | 中南大学 | Phosphorus-based biochar material, and preparation and application thereof |
| CN106565046A (en) * | 2016-08-31 | 2017-04-19 | 惠州市惠阳区力行环保有限公司 | Treatment method for waste cutting fluid |
| CN107176698A (en) * | 2017-07-13 | 2017-09-19 | 河海大学 | A kind of activated carbon of removal farmland water-break agricultural chemicals and microorganism coupling device |
| CN108060157A (en) * | 2018-01-22 | 2018-05-22 | 武汉净宇微藻科技有限公司 | It is a kind of for immobilized bacterium ball of sewage disposal and preparation method thereof |
| CN108467121A (en) * | 2018-05-14 | 2018-08-31 | 胡敏 | A kind of microorganism sewage water processing method being inoculated with flora using activated sludge |
| CN108485993A (en) * | 2018-07-11 | 2018-09-04 | 佛山皖阳生物科技有限公司 | A kind of preparation method of biological adsorption ocean black-koji mould pompon |
-
2011
- 2011-11-09 CN CN 201110351867 patent/CN103102015A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104671437A (en) * | 2015-03-03 | 2015-06-03 | 南华大学 | Method for remedying U (VI) polluted water body by decomposing ground phosphate rock with Aspergillus niger |
| CN104671437B (en) * | 2015-03-03 | 2016-08-24 | 南华大学 | One utilizes aspergillus niger decomposing rock phosphate with hydrogen to repair U(VI) method of polluted-water |
| CN105598158A (en) * | 2016-01-28 | 2016-05-25 | 中南大学 | Phosphorus-based biochar material, and preparation and application thereof |
| CN106565046A (en) * | 2016-08-31 | 2017-04-19 | 惠州市惠阳区力行环保有限公司 | Treatment method for waste cutting fluid |
| CN107176698A (en) * | 2017-07-13 | 2017-09-19 | 河海大学 | A kind of activated carbon of removal farmland water-break agricultural chemicals and microorganism coupling device |
| CN107176698B (en) * | 2017-07-13 | 2019-08-06 | 河海大学 | A kind of active carbon and microorganism coupling device removing farmland water-break pesticide |
| CN108060157A (en) * | 2018-01-22 | 2018-05-22 | 武汉净宇微藻科技有限公司 | It is a kind of for immobilized bacterium ball of sewage disposal and preparation method thereof |
| CN108467121A (en) * | 2018-05-14 | 2018-08-31 | 胡敏 | A kind of microorganism sewage water processing method being inoculated with flora using activated sludge |
| CN108467121B (en) * | 2018-05-14 | 2020-11-10 | 金涛 | Microbial sewage treatment method adopting activated sludge to inoculate flora |
| CN108485993A (en) * | 2018-07-11 | 2018-09-04 | 佛山皖阳生物科技有限公司 | A kind of preparation method of biological adsorption ocean black-koji mould pompon |
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Application publication date: 20130515 |