CN112998270A - Preparation method of astaxanthin microcapsules - Google Patents

Preparation method of astaxanthin microcapsules Download PDF

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Publication number
CN112998270A
CN112998270A CN202110389033.XA CN202110389033A CN112998270A CN 112998270 A CN112998270 A CN 112998270A CN 202110389033 A CN202110389033 A CN 202110389033A CN 112998270 A CN112998270 A CN 112998270A
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astaxanthin
oil
mixing
cyclodextrin
microcapsule
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郭红星
周尽学
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Hainan Sanyuanxing Biotechnology Co ltd
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Hainan Sanyuanxing Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/03—Organic compounds
    • A23L29/035—Organic compounds containing oxygen as heteroatom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/10—Foods or foodstuffs containing additives; Preparation or treatment thereof containing emulsifiers
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A23L29/35—Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/30—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation
    • A23L5/32—Physical treatment, e.g. electrical or magnetic means, wave energy or irradiation using phonon wave energy, e.g. sound or ultrasonic waves
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P10/00—Shaping or working of foodstuffs characterised by the products
    • A23P10/30—Encapsulation of particles, e.g. foodstuff additives
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0203—Solvent extraction of solids with a supercritical fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J13/00—Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
    • B01J13/02—Making microcapsules or microballoons
    • B01J13/04—Making microcapsules or microballoons by physical processes, e.g. drying, spraying
    • B01J13/043—Drying and spraying
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The invention provides a preparation method of an astaxanthin microcapsule, which comprises the following steps: s1, utilizing supercritical CO2Extracting the haematococcus pluvialis by using a fluid extraction method to obtain an astaxanthin extract; s2, mixing the astaxanthin extract, emulsifier and water and carrying out ultrasonic treatmentEmulsifying to obtain astaxanthin oil; s3, mixing the astaxanthin oil with a surfactant to obtain an oil phase core material; s4, mixing the oil phase core material and the water phase wall material, embedding by spray drying, and then freeze-drying to obtain astaxanthin microcapsules; the water phase wall material is a mixture of maltodextrin and hydroxypropyl-beta-cyclodextrin. The preparation method can effectively improve the stability of the astaxanthin microcapsule, and is simple and feasible and low in cost.

Description

Preparation method of astaxanthin microcapsules
Technical Field
The invention relates to the technical field of microcapsule preparation, in particular to a preparation method of an astaxanthin microcapsule.
Background
Astaxanthin, also known as astaxanthin and lobster shell pigment, is a carotenoid, and is also the highest-grade product synthesized from the carotenoid, and is dark pink, and the chemical structure of the astaxanthin is similar to that of beta-carotene. Beta-carotene, lutein, canthaxanthin, lycopene and the like are all intermediate products of carotenoid synthesis, so that astaxanthin has the strongest oxidation resistance in the nature. The astaxanthin has the advantages that the astaxanthin is widely existed in the biological world, particularly, the content of feathers of shrimps, crabs, fishes, algae, yeasts and birds is high, the astaxanthin is one of main carotenoids in marine organisms, the antioxidant capacity of the astaxanthin is 10 times that of beta-carotene and 500 times that of vitamin E, the astaxanthin is called super vitamin E, and the research of Hawaii university and cooperative company shows that the astaxanthin can promote the expression of longevity gene FOXO3, and is expected to help human beings to resist aging.
The existing microcapsule processing and preparation technology can be roughly divided into three major categories, namely a chemical method, a physical method and a physicochemical method, wherein the chemical method mainly comprises an emulsification method, an interfacial polymerization method, an orifice method, a radiation chemical method and the like; the physical methods mainly comprise an extrusion method, a spray drying method, an electrostatic combination method, a vapor deposition method, an air suspension method, a molecular embedding method and the like; the physical and chemical methods include a phase separation method, a dry bath method, an interfacial deposition method, and the like.
The existing microcapsule colloid has poor water solubility, low stability and easy quality change, and the existing astaxanthin oil embedding technology uses an emulsification method to treat the microcapsule colloid, but the oil consumption is overlarge, the cost is higher, and the existing embedding rate is lower, so the production cost is high.
Disclosure of Invention
In view of the above, the present invention aims to provide a method for preparing astaxanthin microcapsules. The preparation method provided by the invention can effectively improve the stability of the astaxanthin microcapsule and reduce the process cost.
The invention provides a preparation method of an astaxanthin microcapsule, which comprises the following steps:
s1, utilizing supercritical CO2Extracting the haematococcus pluvialis by using a fluid extraction method to obtain an astaxanthin extract;
s2, mixing the astaxanthin extract, an emulsifier and water, and ultrasonically emulsifying to obtain astaxanthin oil;
s3, mixing the astaxanthin oil with a surfactant to obtain an oil phase core material;
s4, mixing the oil phase core material and the water phase wall material, embedding by spray drying, and then freeze-drying to obtain astaxanthin microcapsules;
the water phase wall material is a mixture of maltodextrin and hydroxypropyl-beta-cyclodextrin.
Preferably, in step S2, the emulsifier is sucrose fatty acid ester and β -cyclodextrin.
Preferably, in step S2, the amounts of the components are as follows:
0.05g of astaxanthin extract, 0.1g of sucrose fatty acid ester, 0.2g of beta-cyclodextrin and 50mL of water.
Preferably, in step S2, the power of the ultrasonic emulsification is 100W, and the ultrasonic time is 10 min.
Preferably, in the step S3, the surfactant is polysorbate-80.
Preferably, in step S3, the mass ratio of the astaxanthin oil to the surfactant is 4.76: 0.87.
Preferably, in step S3, the mass fraction of the astaxanthin oil in the astaxanthin microcapsule is 4.76%.
Preferably, in the step S4, the mass ratio of the maltodextrin to the hydroxypropyl- β -cyclodextrin is 1: 3.
Preferably, in the step S4, the embedding is performed under a condition of pH 8 to 10.
Preferably, in the step S1, the supercritical CO2The conditions of the fluid extraction method are as follows: the temperature is 31 ℃, and the pressure is 7.3 MPa.
The preparation method of the astaxanthin microcapsule provided by the invention comprises the following steps: s1, utilizing supercritical CO2Extracting the haematococcus pluvialis by using a fluid extraction method to obtain an astaxanthin extract; s2, mixing the astaxanthin extract, an emulsifier and water, and ultrasonically emulsifying to obtainAstaxanthin oil; s3, mixing the astaxanthin oil with a surfactant to obtain an oil phase core material; s4, mixing the oil phase core material and the water phase wall material, embedding by spray drying, and then freeze-drying to obtain astaxanthin microcapsules; the water phase wall material is a mixture of maltodextrin and hydroxypropyl-beta-cyclodextrin. The preparation method can effectively improve the stability of the astaxanthin microcapsule, and is simple and feasible and low in cost.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
Fig. 1 is a schematic flow diagram of a preparation process and a subsequent link of an astaxanthin microcapsule provided by the invention.
Detailed Description
The invention provides a preparation method of an astaxanthin microcapsule, which comprises the following steps:
s1, utilizing supercritical CO2Extracting the haematococcus pluvialis by using a fluid extraction method to obtain an astaxanthin extract;
s2, mixing the astaxanthin extract, an emulsifier and water, and ultrasonically emulsifying to obtain astaxanthin oil;
s3, mixing the astaxanthin oil with a surfactant to obtain an oil phase core material;
s4, mixing the oil phase core material and the water phase wall material, embedding by spray drying, and then freeze-drying to obtain astaxanthin microcapsules;
the water phase wall material is a mixture of maltodextrin and hydroxypropyl-beta-cyclodextrin.
Referring to fig. 1, fig. 1 is a schematic flow chart of a preparation process and a subsequent link of an astaxanthin microcapsule provided by the invention.
Regarding step S1:by using supercritical CO2Extracting the haematococcus pluvialis by a fluid extraction method to obtain an astaxanthin extract.
The invention takes the rain-sound red algae ball as an extraction object and passes through supercritical CO2Extracting astaxanthin from the water by a fluid extraction method. In the present invention, the supercritical CO2The conditions of the fluid extraction process are preferably: the temperature is 31 ℃, and the pressure is 7.3 MPa. And (3) under the supercritical condition, mixing the supercritical fluid with the haematococcus pluvialis to extract the astaxanthin. Specifically, under the supercritical conditions, after the supercritical fluid is contacted with the haematococcus pluvialis, the components with large and small polarity, boiling point and molecular weight are selectively and sequentially extracted, then the supercritical fluid is changed into common gas by means of decompression and heating, and the extracted substances are completely or basically separated out, so that the purposes of separation and purification are achieved, and the purity of the astaxanthin is ensured. And (4) performing the extraction treatment to obtain the astaxanthin extract.
Regarding steps S2 to S3: mixing the astaxanthin extract, an emulsifier and water, and ultrasonically emulsifying to obtain astaxanthin oil. And mixing the astaxanthin oil with a surfactant to obtain an oil phase core material.
In the present invention, the emulsifier is preferably sucrose fatty acid ester and β -cyclodextrin. The mass ratio of the sucrose fatty acid ester to the beta-cyclodextrin is preferably 1: 2.
In the invention, the dosage of the materials is preferably matched as follows: 0.05g of astaxanthin extract, 0.1g of sucrose fatty acid ester, 0.2g of beta-cyclodextrin and 50mL of water.
In the present invention, the conditions of the ultrasonic emulsification are preferably: the power is 100W and the time is 10 min. The astaxanthin oil was obtained by the ultrasonic emulsification treatment.
In the present invention, the surfactant is preferably polysorbate-80. The mass ratio of the astaxanthin oil to the surfactant is preferably 4.76: 0.87. And mixing the astaxanthin oil with a surfactant to obtain an oil phase core material.
Regarding step S4: and mixing the oil phase core material and the water phase wall material, embedding by spray drying, and then freeze drying to obtain the astaxanthin microcapsule.
In the invention, the oil-soluble core material needs to be matched with a water-soluble wall material. In the invention, in order to improve the stability of the astaxanthin oil, the water phase wall material is a mixture of maltodextrin and hydroxypropyl-beta-cyclodextrin. The mass ratio of the maltodextrin to the hydroxypropyl-beta-cyclodextrin is preferably 1: 3.
In the invention, when the oil phase core material and the water phase wall material are mixed and embedded, the embedding is preferably carried out under the weak base condition, and particularly under the condition that the pH value is 8-10. In the present invention, in the embedding, the mass fraction of the astaxanthin oil in the bulk mixture is 4.76%, the mass fraction of the surfactant in the bulk mixture is 0.87%, and the solid content concentration of the bulk mixture is 0.20g/mol (that is, the mass fractions of the astaxanthin oil and the surfactant in the final astaxanthin microcapsule are 4.76% and 0.87%, respectively).
In the present invention, the embedding is carried out by spray drying. The spray drying is a process of dispersing feed liquid into fog drops by using an atomizer, and then directly contacting the fog drops with hot air or other gases to dry the fog drops into powder and granular products, the fog drops are usually dried by two processes, namely a first drying process, namely constant-speed drying and a second drying process, namely speed reduction drying, the mass fraction of astaxanthin is 4.76% during embedding, meanwhile, the mass fraction of added polysorbate-80 is 0.87%, the solid concentration is 0.20g/mol, and the process needs to be carried out under the state of weak base (the PH is 8-10) during embedding, so that the stability of microcapsule colloid is improved. The spray drying method is simple in production operation, easy to accept by production technicians, capable of realizing small-batch or large-batch continuous production, free of special equipment, economical and simple, suitable for production of powdered oil and essence in the food industry, free of waste and wastewater in production and one of the most environment-friendly production methods, and the production equipment is common equipment in the food industry, so that industrial production is easy to realize.
In the present invention, the above-mentioned embedding is followed by freeze-drying. In the invention, EDTA (ethylene diamine tetraacetic acid) is preferably used as a freeze-drying protective agent in the freeze drying process, so that the stability of the EDTA can be effectively protected. After the freeze-drying, astaxanthin microcapsules were obtained.
In the invention, after the astaxanthin microcapsules are obtained, the following steps are carried out: and (5) inspecting, storing and transporting.
In the present invention, the items of the inspection include: appearance, moisture, astaxanthin content, free astaxanthin content, heavy metals and harmful elements, total number of colonies, number of molds and yeasts, Escherichia coli, Salmonella. The produced astaxanthin is inspected, the qualification rate of the product can be found in time, the unqualified product is prevented from flowing to the market to cause adverse effect, a sampling method of systematic sampling is adopted for inspection, a rotary evaporator and an ultrasonic instrument are adopted for inspecting the astaxanthin content, and the astaxanthin content can be calculated according to a detected chromatographic column, a detected mobile phase, a detected column temperature, a detected wavelength, a detected flow rate and a detected sample volume by using absolute ethyl alcohol, trichloromethane, normal hexane and acetone as reagents; the heavy metal and the harmful elements can be detected by an ultraviolet-visible spectrophotometry, the heavy metal can react with the color developing agent, the heavy metal and the color developing agent can generate colored liquid together, and the thicker the color is, the higher the heavy metal content is; the bacterial colony is detected by taking out a sample and culturing and observing the sample through a culture medium, the system sampling is easy to understand, the system sampling is simple and easy to implement, a sample which is uniformly distributed in the whole is easy to obtain, and the sampling error is smaller than that of pure random sampling.
In the present invention, with regard to storage: the astaxanthin stacking machine is placed in a dry, ventilated, shady, dark and clean place, the stacking distance is more than 20cm from the wall and the ground, and a spacer is arranged, so that the astaxanthin is prevented from being stacked together with toxic, harmful, perishable, easily polluted and other objects, and the pollution of the environment to the astaxanthin is prevented; the storage selects the lightproof glass bottle with dark color, the glass bottle is sterilized at high temperature, and then the astaxanthin oil is filled in the glass bottle, so that the residual air in the container can be reduced, and the oil is prevented from being oxidized and deteriorated.
In the present invention, with regard to transportation: must lightly load and unload during the transportation, must not carry with poisonous, harmful and easily polluted object mixed loading, prevent to drench strictly, insolate, the glass bottle surface cover that needs to store astaxanthin oil during the transportation is equipped with crashproof cylinder liner, and crashproof cylinder liner can prevent to be broken at the transportation glass bottle, also can prevent to lead to the transportation high temperature sealed with the glass bottle to lead to astaxanthin rotten.
The preparation method provided by the invention has the following beneficial effects:
1. according to the invention, when the astaxanthin oil is prepared by using the sucrose fatty acid ester and beta-cyclodextrin compound, 0.05g of astaxanthin extract, 0.1g of sucrose fatty acid ester, 0.2g of beta-cyclodextrin, 50ml of water, 100W of ultrasonic power and 10min of ultrasonic time, the astaxanthin can be best extracted from the astaxanthin extract, and waste is prevented.
2. According to the invention, the stability of the astaxanthin oil can be improved by embedding in a weak base environment, 0.87% of polysorbate-80 is added, the concentration of solid matters is 0.20g/mol, the embedding rate can be improved, and EDTA as a cold drying protective agent can play a role in protecting the stability of the astaxanthin oil in the drying process and prevent the astaxanthin oil from deteriorating.
3. The method carries out the microcapsule embedding by using the spray drying method, has simple production operation, is easy to be accepted by production technicians, can carry out continuous production in small batch or large batch, does not need special equipment, is economic and simple, does not generate waste and wastewater in the production, is environment-friendly and sanitary, and saves the cost.
For a further understanding of the invention, reference will now be made to the preferred embodiments of the invention by way of example, and it is to be understood that the description is intended to further illustrate features and advantages of the invention, and not to limit the scope of the claims.
Example 1
S1, utilizing supercritical CO2Extracting the haematococcus pluvialis by a fluid extraction method to obtain an astaxanthin extract. The supercritical extraction method comprises the following conditions: the temperature is 31 ℃, and the pressure is 7.3 MPa. Mixing the supercritical fluid with the haematococcus pluvialis under the supercritical condition to extract the astaxanthin to obtain the astaxanthin extract.
S2, taking 0.05g of astaxanthin extract, 0.1g of sucrose fatty acid ester, 0.2g of beta-cyclodextrin and 50mL of water, and carrying out ultrasonic emulsification under the conditions that: the power is 100W, and the time is 10min, so that the astaxanthin oil is obtained.
S3, mixing the astaxanthin oil and polysorbate-80 according to the mass ratio of 4.76: 0.87 to obtain the oil phase core material.
S4, mixing maltodextrin and hydroxypropyl-beta-cyclodextrin according to the mass ratio of 1: 3 to obtain the water phase wall material.
And S5, mixing the oil phase core material and the water phase wall material, embedding by spray drying, and then freeze-drying to obtain the astaxanthin microcapsule.
Wherein, the embedding is carried out under the condition that the pH value is 9, in the embedding, the mass fraction of the astaxanthin oil in the whole mixed material is 4.76%, the mass fraction of the surfactant in the whole mixed material is 0.87%, and the solid concentration of the whole mixed material is 0.20 g/mol. In the freeze drying, EDTA is used as a cold drying protective agent.
The stability of the astaxanthin microcapsule is detected, and the result shows that the astaxanthin microcapsule prepared by the method has excellent stability.
The foregoing examples are provided to facilitate an understanding of the principles of the invention and their core concepts, including the best mode, and also to enable any person skilled in the art to practice the invention, including making and using any devices or systems and performing any incorporated methods. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention. The scope of the invention is defined by the claims and may include other embodiments that occur to those skilled in the art. Such other embodiments are intended to be within the scope of the claims if they have structural elements that approximate the literal language of the claims, or if they include equivalent structural elements with insubstantial differences from the literal languages of the claims.

Claims (10)

1. The preparation method of the astaxanthin microcapsule is characterized by comprising the following steps:
s1, utilizing supercritical CO2Extracting the haematococcus pluvialis by using a fluid extraction method to obtain an astaxanthin extract;
s2, mixing the astaxanthin extract, an emulsifier and water, and ultrasonically emulsifying to obtain astaxanthin oil;
s3, mixing the astaxanthin oil with a surfactant to obtain an oil phase core material;
s4, mixing the oil phase core material and the water phase wall material, embedding by spray drying, and then freeze-drying to obtain astaxanthin microcapsules;
the water phase wall material is a mixture of maltodextrin and hydroxypropyl-beta-cyclodextrin.
2. The method according to claim 1, wherein in step S2, the emulsifier is sucrose fatty acid ester and β -cyclodextrin.
3. The method according to claim 2, wherein in step S2, the following substances are used:
0.05g of astaxanthin extract, 0.1g of sucrose fatty acid ester, 0.2g of beta-cyclodextrin and 50mL of water.
4. The method according to claim 1, wherein in step S2, the power of the ultrasonic emulsification is 100W, and the ultrasonic time is 10 min.
5. The method according to claim 1, wherein in step S3, the surfactant is polysorbate-80.
6. The method according to claim 1 or 5, wherein in step S3, the ratio of astaxanthin oil to surfactant is 4.76: 0.87 by mass.
7. The production method according to claim 6, wherein in the step S3, the mass fraction of the astaxanthin oil in the astaxanthin microcapsule is 4.76%.
8. The method according to claim 1, wherein in the step S4, the mass ratio of the maltodextrin to the hydroxypropyl- β -cyclodextrin is 1: 3.
9. The method according to claim 1, wherein the embedding is performed at a pH of 8 to 10 in step S4.
10. The method according to claim 1, wherein in the step S1, the supercritical CO is used2The conditions of the fluid extraction method are as follows: the temperature is 31 ℃, and the pressure is 7.3 MPa.
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