CN113214998A - Preparation method of natural astaxanthin - Google Patents
Preparation method of natural astaxanthin Download PDFInfo
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- CN113214998A CN113214998A CN202110665104.4A CN202110665104A CN113214998A CN 113214998 A CN113214998 A CN 113214998A CN 202110665104 A CN202110665104 A CN 202110665104A CN 113214998 A CN113214998 A CN 113214998A
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- haematococcus pluvialis
- natural astaxanthin
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- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 title claims abstract description 25
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 title claims abstract description 25
- 229940022405 astaxanthin Drugs 0.000 title claims abstract description 25
- 235000013793 astaxanthin Nutrition 0.000 title claims abstract description 25
- 239000001168 astaxanthin Substances 0.000 title claims abstract description 25
- 238000002360 preparation method Methods 0.000 title description 2
- 230000001954 sterilising effect Effects 0.000 claims abstract description 21
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 17
- 241000168517 Haematococcus lacustris Species 0.000 claims abstract description 15
- 241000195493 Cryptophyta Species 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 11
- 230000004992 fission Effects 0.000 claims abstract description 8
- 238000005286 illumination Methods 0.000 claims abstract description 4
- 238000011081 inoculation Methods 0.000 claims abstract description 4
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 18
- 238000000227 grinding Methods 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 229910052757 nitrogen Inorganic materials 0.000 claims description 9
- 238000000605 extraction Methods 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 238000004519 manufacturing process Methods 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000000194 supercritical-fluid extraction Methods 0.000 claims description 4
- 230000000694 effects Effects 0.000 abstract description 15
- 238000001035 drying Methods 0.000 abstract description 8
- 235000015097 nutrients Nutrition 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 abstract description 2
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 230000004071 biological effect Effects 0.000 abstract description 2
- 210000000170 cell membrane Anatomy 0.000 abstract description 2
- 238000010438 heat treatment Methods 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 230000036541 health Effects 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 210000002969 egg yolk Anatomy 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 235000021466 carotenoid Nutrition 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 125000001895 carotenoid group Chemical group 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- 235000013345 egg yolk Nutrition 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 241000238565 lobster Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C403/00—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone
- C07C403/24—Derivatives of cyclohexane or of a cyclohexene or of cyclohexadiene, having a side-chain containing an acyclic unsaturated part of at least four carbon atoms, this part being directly attached to the cyclohexane or cyclohexene or cyclohexadiene rings, e.g. vitamin A, beta-carotene, beta-ionone having side-chains substituted by six-membered non-aromatic rings, e.g. beta-carotene
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C2601/00—Systems containing only non-condensed rings
- C07C2601/12—Systems containing only non-condensed rings with a six-membered ring
- C07C2601/16—Systems containing only non-condensed rings with a six-membered ring the ring being unsaturated
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
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- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
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- General Engineering & Computer Science (AREA)
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- General Chemical & Material Sciences (AREA)
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Abstract
The invention belongs to the technical field of chemical industry, and particularly relates to a method for preparing natural astaxanthin, which comprises the following steps: performing fission propagation, namely storing haematococcus pluvialis algae seeds in an algae seed photosynthetic reactor for fission propagation under the culture conditions of pH 8.0, illumination intensity of 1klx, continuous culture for 24 hours, temperature of 24 ℃ and inoculation amount of 1.95 multiplied by 105mL < -1 >; according to the invention, by setting microwave sterilization, nutrient components can be effectively maintained, the microwave has double sterilization functions of quick heating of thermal effect and non-thermal effect (biological effect: the microwave quickly breaks down bacterial cell membrane and bacteria die): compared with the conventional thermal drying and sterilization, the required drying and sterilization effects can be obtained at a lower temperature and in a shorter time, and meanwhile, the energy is saved.
Description
Technical Field
The invention belongs to the technical field of chemical industry, and particularly relates to a method for preparing natural astaxanthin.
Background
Astaxanthin also called astaxanthin and lobster shell pigment is carotenoid and is the highest-grade product synthesized by the carotenoid, in daily life, people can obtain astaxanthin by eating poultry which takes in algae and fungi containing astaxanthin, but the astaxanthin content obtained by the method is low and can not achieve the health care effect, haematococcus pluvialis is the only astaxanthin source which is directly used by people and accepted by China ministry of health and major national health departments all over the world, so haematococcus pluvialis oil products containing astaxanthin appear in the market, but the molecular structure of the astaxanthin contained in the products is not easily absorbed by the digestive system and skin of the human body directly, the health care effect is common, in addition, the yolk oil products containing astaxanthin also appear in the market, the traditional yolk oil products are prepared by taking cooked egg yolk as a production raw material in a high-temperature baking and high-temperature supercritical extraction mode, but the extraction effect of the astaxanthin is poor under the high-temperature condition, the activity of the extracted astaxanthin is low, and the health care effect is general.
Disclosure of Invention
To solve the problems set forth in the background art described above. The invention provides a method for preparing natural astaxanthin, which has the characteristic of poor extraction effect.
In order to achieve the purpose, the invention provides the following technical scheme: a method for producing natural astaxanthin, comprising the steps of:
the method comprises the following steps: performing fission propagation, namely continuously culturing at the temperature of 24 ℃ and the inoculation amount of 1.95 multiplied by 105mL < -1 > for 24h under the conditions of pH 8.0 and illumination intensity of 1klx, and storing haematococcus pluvialis algae seeds in an algae seed photosynthetic reactor for fission propagation;
step two: artificially intervening to induce Haematococcus pluvialis and find that natural astaxanthin is accumulated when the concentration is 1mmol/LNaN3, wherein the color of the Haematococcus pluvialis is changed from green to red;
step three: physical sterilization, namely storing haematococcus pluvialis strains into a sealed reaction kettle for microwave sterilization, so that the nutrient components are effectively maintained, and the required drying and sterilizing effects can be obtained at a lower temperature and in a shorter time compared with the conventional thermal drying and sterilizing;
step four: grinding and crushing, adding a proper amount of liquid nitrogen for grinding, wherein the temperature of the liquid nitrogen is 196 ℃, the liquid nitrogen is volatilized to ensure that the ground object becomes brittle and the grinding time is shortened, no chemical reagent is added in the wall breaking process, no pollution is caused, and then a proper amount of ethanol is added for mixing and stirring, so that the activity of the algae seeds is maintained to the maximum extent.
Step five: freeze drying, namely performing low-temperature freeze drying on the mixed solution after wall breaking, wherein the temperature is controlled to be-10 to-15 ℃, and the time is controlled within 2 h;
step six: extracting, storing into a supercritical extraction kettle for extraction;
step seven: and (4) performing centrifugal filtration, namely performing centrifugal filtration on the extracted mixed solution to obtain a finished product.
Preferably, the sterilization temperature in the third step is controlled to be 65-70 ℃, and the time is controlled to be 8-10 min.
Preferably, the mixing and stirring temperature in the fourth step is controlled to be-18 ℃ and the time is controlled to be within 24 hours.
Compared with the prior art, the invention has the beneficial effects that:
1. according to the invention, by setting microwave sterilization, nutrient components can be effectively maintained, the microwave has double sterilization functions of quick heating of thermal effect and non-thermal effect (biological effect: the microwave quickly breaks down bacterial cell membrane and bacteria die): compared with the conventional thermal drying and sterilization, the required drying and sterilization effects can be obtained at a lower temperature and in a shorter time, and meanwhile, the energy is saved.
2. According to the method, the algae seeds are embrittled by adopting liquid nitrogen grinding, so that the grinding time is shortened, no chemical reagent is added in the wall breaking process, no pollution is generated, the efficiency is higher, the activity of the algae seeds is kept to the maximum extent, and the extraction rate of the astaxanthin is improved.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
The invention provides the following technical scheme: a method for producing natural astaxanthin, comprising the steps of:
the method comprises the following steps: performing fission propagation, namely continuously culturing at the temperature of 24 ℃ and the inoculation amount of 1.95 multiplied by 105mL < -1 > for 24h under the conditions of pH 8.0 and illumination intensity of 1klx, and storing haematococcus pluvialis algae seeds in an algae seed photosynthetic reactor for fission propagation;
step two: artificially intervening to induce Haematococcus pluvialis and find that natural astaxanthin is accumulated when the concentration is 1mmol/LNaN3, wherein the color of the Haematococcus pluvialis is changed from green to red;
step three: physical sterilization, namely storing haematococcus pluvialis strains into a sealed reaction kettle for microwave sterilization, so that the nutrient components are effectively maintained, and the required drying and sterilizing effects can be obtained at a lower temperature and in a shorter time compared with the conventional thermal drying and sterilizing;
step four: grinding and crushing, adding a proper amount of liquid nitrogen for grinding, wherein the temperature of the liquid nitrogen is 196 ℃, the liquid nitrogen is volatilized to ensure that the ground object becomes brittle and the grinding time is shortened, no chemical reagent is added in the wall breaking process, no pollution is caused, and then a proper amount of ethanol is added for mixing and stirring, so that the activity of the algae seeds is maintained to the maximum extent.
Step five: freeze drying, namely performing low-temperature freeze drying on the mixed solution after wall breaking, wherein the temperature is controlled to be-10 to-15 ℃, and the time is controlled within 2 h;
step six: extracting, storing into a supercritical extraction kettle for extraction;
step seven: and (4) performing centrifugal filtration, namely performing centrifugal filtration on the extracted mixed solution to obtain a finished product.
Specifically, the sterilization temperature in the third step is controlled to be 65-70 ℃, and the time is controlled to be 8-10 min.
Specifically, in the fourth step, the mixing and stirring temperature is controlled to be-18 ℃, and the time is controlled within 24 hours.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (3)
1. A method for producing natural astaxanthin, which comprises the steps of:
the method comprises the following steps: performing fission propagation, namely storing haematococcus pluvialis algae seeds in an algae seed photosynthetic reactor for fission propagation under the culture conditions of pH 8.0, illumination intensity of 1klx, continuous culture for 24 hours, temperature of 24 ℃ and inoculation amount of 1.95 multiplied by 105mL < -1 >;
step two: artificially intervening to induce Haematococcus pluvialis and find that natural astaxanthin is accumulated when the concentration is 1mmol/LNaN3, wherein the color of the Haematococcus pluvialis is changed from green to red;
step three: physical sterilization, namely storing haematococcus pluvialis strains into a sealed reaction kettle for microwave sterilization;
step four: grinding and crushing, adding a proper amount of liquid nitrogen for grinding, wherein the temperature of the liquid nitrogen is 196 ℃.
Step five: freeze drying, namely performing low-temperature freeze drying on the mixed solution after wall breaking, wherein the temperature is controlled to be-10 to-15 ℃, and the time is controlled within 2 h;
step six: extracting, storing into a supercritical extraction kettle for extraction;
step seven: and (4) performing centrifugal filtration, namely performing centrifugal filtration on the extracted mixed solution to obtain a finished product.
2. The method for producing natural astaxanthin according to claim 1, wherein: in the third step, the sterilization temperature is controlled to be 65-70 ℃, and the time is controlled to be 8-10 min.
3. The method for producing natural astaxanthin according to claim 1, wherein: in the fourth step, the mixing and stirring temperature is controlled to be-18 ℃ and the time is controlled within 24 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110665104.4A CN113214998A (en) | 2021-06-16 | 2021-06-16 | Preparation method of natural astaxanthin |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110665104.4A CN113214998A (en) | 2021-06-16 | 2021-06-16 | Preparation method of natural astaxanthin |
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| CN113214998A true CN113214998A (en) | 2021-08-06 |
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| CN202110665104.4A Pending CN113214998A (en) | 2021-06-16 | 2021-06-16 | Preparation method of natural astaxanthin |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102337215A (en) * | 2011-10-20 | 2012-02-01 | 烟台华融生物科技有限公司 | Methods for culturing haematococcus pluvialis and producing astaxanthin |
| CN103989031A (en) * | 2014-06-09 | 2014-08-20 | 嘉兴泽元生物制品有限责任公司 | Method for controlling microbes in algae powder by combination of microwave sterilization and spray drying |
| CN104212865A (en) * | 2014-09-15 | 2014-12-17 | 李健 | Production process for producing astaxanthin by micro-alga culture |
| CN104232720A (en) * | 2014-08-26 | 2014-12-24 | 山东金晶生物技术有限公司 | Method for producing astaxanthin by inducing Haematococcus pluvialis |
| CN104529852A (en) * | 2014-12-10 | 2015-04-22 | 青岛无为保温材料有限公司 | Method for extracting astaxanthin from haematococcus pluvialis |
| CN105755088A (en) * | 2016-05-18 | 2016-07-13 | 彭小伟 | Method for inducing haematococcus pluvialis to produce C40H52O4 |
| CN205459993U (en) * | 2016-01-21 | 2016-08-17 | 云南麟珑微藻科技有限责任公司 | Red ball algae sterilization apparatus is given birth to to rain |
-
2021
- 2021-06-16 CN CN202110665104.4A patent/CN113214998A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102337215A (en) * | 2011-10-20 | 2012-02-01 | 烟台华融生物科技有限公司 | Methods for culturing haematococcus pluvialis and producing astaxanthin |
| CN103989031A (en) * | 2014-06-09 | 2014-08-20 | 嘉兴泽元生物制品有限责任公司 | Method for controlling microbes in algae powder by combination of microwave sterilization and spray drying |
| CN104232720A (en) * | 2014-08-26 | 2014-12-24 | 山东金晶生物技术有限公司 | Method for producing astaxanthin by inducing Haematococcus pluvialis |
| CN104212865A (en) * | 2014-09-15 | 2014-12-17 | 李健 | Production process for producing astaxanthin by micro-alga culture |
| CN104529852A (en) * | 2014-12-10 | 2015-04-22 | 青岛无为保温材料有限公司 | Method for extracting astaxanthin from haematococcus pluvialis |
| CN205459993U (en) * | 2016-01-21 | 2016-08-17 | 云南麟珑微藻科技有限责任公司 | Red ball algae sterilization apparatus is given birth to to rain |
| CN105755088A (en) * | 2016-05-18 | 2016-07-13 | 彭小伟 | Method for inducing haematococcus pluvialis to produce C40H52O4 |
Non-Patent Citations (1)
| Title |
|---|
| 孙延红: ""雨生红球藻诱变育种及突变株的筛选"", 《中国优秀硕士学位论文全文数据库农业科技辑》 * |
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