CN105018353A - Preparation method for monascus yellow pigment - Google Patents
Preparation method for monascus yellow pigment Download PDFInfo
- Publication number
- CN105018353A CN105018353A CN201510441912.7A CN201510441912A CN105018353A CN 105018353 A CN105018353 A CN 105018353A CN 201510441912 A CN201510441912 A CN 201510441912A CN 105018353 A CN105018353 A CN 105018353A
- Authority
- CN
- China
- Prior art keywords
- monascus
- yellow pigment
- preparation
- strain
- monascus yellow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention belongs to the field of food additives, and concretely relates to a preparation method for monascus yellow pigment. The preparation method for monascus yellow pigment comprises the following steps: (1) bacterial strain breeding; (2) strain culturing; (3) fermentation; (4) filtering; (5) extraction; (6) concentration; and (7) drying. The beneficial effects comprise that a dominant strain with strong and fast yellow-pigment-producing capability is obtained through the method; and then a method that the dominant strain is used for liquid deep fermentation is employed for extracting monascus yellow pigment, and the obtained monascus yellow pigment obtained through the method is high in color value (>= 300 U/g), low in citrinin content (<= 50 U/Kg), strong in light resistance, not easy to fade, and is stable to metal ions. Monascus yellow pigment obtained through the method is low in cost, and is safe and nontoxic.
Description
Technical field
The invention belongs to foodstuff additive field, be specifically related to a kind of preparation method of monascus yellow pigment.
Background technology
Food dye is the indispensable class additive of foodstuffs industry, pharmaceutical industry and daily chemical industry.In recent years, due to safety issue, some synthetic colours are no longer applicable to foodstuffs industry.Therefore, the exploitation of natural pigment is subject to people's attention day by day.
Monascorubin is the secondary metabolite secreted in monascus growth metabolism, has the feature that protein tinctorial property is good, stability is high, security is high, has the biologic activity such as antibacterial, adjusting blood lipid, prevention of arterial sclerosis simultaneously.Therefore, monascorubin is extensively favored in foodstuff production market.In foodstuff production, mainly utilize its haematochrome to apply as tinting material at present, yellow pigment is underutilized.And the natural yellow pigment having nutritional health function concurrently is the inexorable trend of pigment development, 60% of the market requirement can be accounted for, there is wide exploitation strength and prospect.
At present for the preparation method of monascus yellow pigment, be all generally change monascorubin into monascus yellow pigment.Chinese invention patent publication number CN103074640A, disclose a kind of method of direct electroreduction monascorubin synthesis monascus yellow pigment, the method take monascorubin as raw material, utilize the principle of Organic electro-synthesis, according to organism colouration mechanism, be reagent with electronics, the double bond in the cyclic conjugated system of monascorubin is reduced, make the maximum absorption wavelength blue shift of compound, thus obtain monascus yellow pigment.Chinese invention patent publication number CN101880469A, disclose a kind of preparation method of water-soluble monascus yellow pigment, the soluble red rice red haematochrome of alcohol that the method produces with Monascus anka Nakazawa et sato metabolism is for raw material, pass through sulfonation reaction, through centrifugal or filtration, washing of precipitate, alkaline hydrolysis, neutralization, drying, pulverizing, obtained water-soluble monascus yellow pigment.These two kinds of methods all adopt chemical process to obtain monascus yellow pigment, and the pure natural monascus yellow pigment adopting biological method obtained, domesticly have not been reported.
Summary of the invention
In order to solve above-mentioned technical problem, the invention provides a kind of look valency high (>=300U/g), citrinin content low (≤50U/Kg), photostabilization are strong, not fugitive color, stable to metal ion, cost is low, the preparation method of the monascus yellow pigment of safety non-toxic.
The preparation method of monascus yellow pigment of the present invention, comprises following step:
(1) strain improvement
Select by mutagenesis be separated obtain monascus (
monascus anka), select and produce the strong and fast strain excellent of yellow pigment ability, above-mentioned monascus is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 30th, 2015, and deposit number is CGMCC NO.10909, and preservation address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City;
(2) seed culture
By in the slant medium of the inoculation chosen in step (1) after sterilizing, at 30-37 DEG C, in shaking table, cultivate 8-12d;
(3) ferment: be inoculated in following fermention medium by excellent monascus specie cultured in step (2), described cultivation is specially: 4-8% Semen Maydis powder, 1-5% glucose, 0.1-0.5%(NH
4)
2sO
4, 0.05-0.15KH
2pO
4, 0.05-0.15MgSO
4, shake-flask culture 4-6d, culture temperature 30-37 DEG C, add 0.3-0.5% glucose solution at 3-5d every day, obtains fermented liquid after fermentation;
(4) filter: add hydrochloric acid and regulate fermented liquid pH2-3, filter after precipitation 4-8h, obtain filter residue;
(5) lixiviate: join in the 60%-80% ethanolic soln of 5-12 times of weight by filtration gained filter residue, stir 1-2h, adopt NaOH regulator solution pH to be 8-13, after soaking 5-12h, ceramic filtration membrane is filtered, and obtains vat liquor;
(6) concentrated: to adopt triple effect multi-step evaporator to concentrate vat liquor, obtain concentrated solution, with salt acid for adjusting pH about 7;
(7) dry: concentrated solution to be carried out spraying dry, obtains monascus yellow pigment powder.
Mutagenesis method in above-mentioned step (1) is physics or ultraviolet mutagenesis.
Mutagenesis method in above-mentioned step (1) is physical mutagenesis, described physical mutagenesis is treated to and adopts peak power 1000w, the household microwave oven of pulse-repetition 2540MHZ, according to 20-60s different time, microwave treatment is carried out to Monascus anka Nakazawa et sato spore suspension, the bacteria suspension drawing 0.1mL is subsequently coated on flat board, subsequently flat board is placed in 15W ultraviolet lamp 30cm place and irradiates 10-30s, immediately wrap up in black paper bag and put 33 DEG C of constant incubators cultivation 7d, select and produce the strong and fast strain excellent of yellow pigment ability.
Preferably, in above-mentioned step (2), at 33 DEG C, in shaking table, 10d is cultivated.
In above-mentioned step (3), substratum is specially: 6% Semen Maydis powder, 3% glucose, 0.3%(NH
4)
2sO
4, 0.1KH
2pO
4, 0.1MgSO
4, shake-flask culture 5d, culture temperature 30-37 DEG C, add 0.4% glucose solution at 3-5d every day, obtains fermented liquid after fermentation.
In above-mentioned step (4), add hydrochloric acid and regulate fermented liquid pH2.5, filter after precipitation 6h, obtain filter residue.
In above-mentioned step (5): join in 70% ethanolic soln of 8 times of weight by filtration gained filter residue, stir 1.5h, adopt NaOH regulator solution pH to be 10, after soaking 8h, ceramic filtration membrane is filtered, and obtains vat liquor.
Step (1) is selected after bacterial strain, then carries out genetic stability test, is specially: the dominant strain continuous passage filtered out cultivates 8 times, the strong and fireballing bacterial strain of the ability selecting to produce yellow pigment.
Beneficial effect of the present invention is, adopts method of the present invention to obtain producing the strong and fast dominant strain of yellow pigment ability; The method adopting this dominant strain to carry out liquid submerged fermentation again extracts monascus yellow pigment, the monascus yellow pigment look valency high (>=300U/g) that the method obtains, citrinin content low (≤50U/Kg), photostabilization are strong, not fugitive color, metal ion is stablized, and the red colouring agent for food, also used as a Chinese medicine Huang Cheng that method of the present invention obtains is originally low, safety non-toxic.
Embodiment
Below in conjunction with specific embodiment, the present invention is further described, so that those skilled in the art more understands the present invention, but does not therefore limit the present invention.
Embodiment 1
A preparation method for monascus yellow pigment, step is as follows:
1) screening of monascus strain
Monascus anka Nakazawa et sato spore suspension prepare: by monascus strain (monascus (
monascus anka) being deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 30th, 2015, deposit number is CGMCC NO.10909; ) blow into granulated glass sphere sterilized water, put shaking table 200r/min, jolting 1h;
Mutagenic treatment: the household microwave oven adopting peak power 1000w, pulse-repetition 2540MHZ, mid power intensity, according to 20-60s different time, carries out microwave treatment to Monascus anka Nakazawa et sato spore suspension, and the bacteria suspension drawing 0.1mL is coated on flat board.Subsequently flat board is placed in 15W ultraviolet lamp 30cm place and irradiates 15s, wrap up in black paper bag immediately and put 33 DEG C of constant incubators cultivation 7d, select and produce the dark and fast strain excellent of yellow pigment color;
The genetic stability test of mutant strain: the dominant strain continuous passage filtered out cultivates 8 times, observes the ability and speed of producing yellow pigment.
2) extraction of monascus yellow pigment
Cultivate: the mutant strain of high yield yellow pigment being inoculated into becoming after sterilizing is in the slant medium of 4% maltose, 4% Zulkovsky starch, 3% peptone, 2% agar, is cultivate 10d in the shaking table of 33 DEG C in temperature;
Fermentation: cultured excellent monascus specie being inoculated into composition is 6% Semen Maydis powder, 3% glucose, 0.3%(NH
4)
2sO
4, 0.1KH
2pO
4, 0.1MgSO
4fermention medium, carry out shake-flask culture 5d, culture temperature about 33 DEG C, adds 0.4% glucose solution at 3-5d every day, after fermentation fermented liquid;
Filter: add hydrochloric acid and regulate fermented liquid pH2, filter after precipitation 4h, obtain filter residue;
Lixiviate: join in 70% ethanolic soln of 10 times of weight by filtration gained filter residue, stir 1-2h, adopt NaOH regulator solution pH to be 9, after soaking 7h, ceramic filtration membrane is filtered, and obtains vat liquor;
Concentrated: to adopt triple effect multi-step evaporator to concentrate vat liquor, obtain concentrated solution, with salt acid for adjusting pH about 7;
Dry: concentrated solution to be carried out spraying dry, obtains monascus yellow pigment powder.
Embodiment 2
A preparation method for monascus yellow pigment, step is as follows:
1) screening of monascus strain
Monascus anka Nakazawa et sato spore suspension prepare: by monascus strain (monascus (
monascus anka) being deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 30th, 2015, deposit number is CGMCC NO.10909; ) blow into granulated glass sphere sterilized water, put shaking table 200r/min, jolting 1h;
Mutagenic treatment: the household microwave oven adopting peak power 1000w, pulse-repetition 2540MHZ, mid power intensity, according to 20-60s different time, carries out microwave treatment to Monascus anka Nakazawa et sato spore suspension, and the bacteria suspension drawing 0.1mL is coated on flat board.Subsequently flat board is placed in 15W ultraviolet lamp 30cm place and irradiates 15s, wrap up in black paper bag immediately and put 33 DEG C of constant incubators cultivation 7d.Select and produce the dark and fast strain excellent of yellow pigment color;
The genetic stability test of mutant strain: the dominant strain continuous passage filtered out cultivates 8 times, observes the ability and speed of producing yellow pigment.
2) extraction of monascus yellow pigment
Cultivate: the mutant strain of high yield yellow pigment being inoculated into becoming after sterilizing is in the slant medium of 4% maltose, 4% Zulkovsky starch, 3% peptone, 2% agar, is cultivate 10d in the shaking table of 33 DEG C in temperature;
Fermentation: cultured excellent monascus specie being inoculated into composition is 6% Semen Maydis powder, 3% glucose, 0.3%(NH
4)
2sO
4, 0.1KH
2pO
4, 0.1MgSO
4fermention medium, carry out shake-flask culture 5d, culture temperature about 33 DEG C, adds 0.4% glucose solution at 3-5d every day, after fermentation fermented liquid;
Filter: add hydrochloric acid and regulate fermented liquid pH2.5, filter after precipitation 4h, obtain filter residue;
Lixiviate: join in 75% ethanolic soln of 8 times of weight by filtration gained filter residue, stir 1-2h, adopt NaOH regulator solution pH to be 10, after soaking 10h, ceramic filtration membrane is filtered, and obtains vat liquor;
Concentrated: to adopt triple effect multi-step evaporator to concentrate vat liquor, obtain concentrated solution, with salt acid for adjusting pH 6.5;
Dry: concentrated solution to be carried out spraying dry, obtains monascus yellow pigment powder.
Embodiment 3
A preparation method for monascus yellow pigment, step is as follows:
1) screening of monascus strain
Monascus anka Nakazawa et sato spore suspension prepare: by monascus strain (monascus (
monascus anka) being deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 30th, 2015, deposit number is CGMCC NO.10909; ) blow into granulated glass sphere sterilized water, put shaking table 200r/min, jolting 1h;
Mutagenic treatment: the household microwave oven adopting peak power 1000w, pulse-repetition 2540MHZ, mid power intensity, according to 20-60s different time, carries out microwave treatment to Monascus anka Nakazawa et sato spore suspension, and the bacteria suspension drawing 0.1mL is coated on flat board.Subsequently flat board is placed in 15W ultraviolet lamp 30cm place and irradiates 15s, wrap up in black paper bag immediately and put 33 DEG C of constant incubators cultivation 7d.Select and produce the dark and fast strain excellent of yellow pigment color;
The genetic stability test of mutant strain: the dominant strain continuous passage filtered out cultivates 8 times, observes the ability and speed of producing yellow pigment;
2) extraction of monascus yellow pigment
Cultivate: the mutant strain of high yield yellow pigment being inoculated into becoming after sterilizing is in the slant medium of 4% maltose, 4% Zulkovsky starch, 3% peptone, 2% agar, is cultivate 10d in the shaking table of 33 DEG C in temperature;
Fermentation: cultured excellent monascus specie being inoculated into composition is 6% Semen Maydis powder, 3% glucose, 0.3%(NH
4)
2sO
4, 0.1KH
2pO
4, 0.1MgSO
4fermention medium, carry out shake-flask culture 5d, culture temperature about 33 DEG C, adds 0.4% glucose solution at 3-5d every day, after fermentation fermented liquid
Filter: add hydrochloric acid and regulate fermented liquid pH3, filter after precipitation 5h, obtain filter residue;
Lixiviate: join in 70% ethanolic soln of 10 times of weight by filtration gained filter residue, stir 1-2h, adopt NaOH regulator solution pH to be 11, after soaking 9h, ceramic filtration membrane is filtered, and obtains vat liquor;
Concentrated: to adopt triple effect multi-step evaporator to concentrate vat liquor, obtain concentrated solution, with salt acid for adjusting pH 7;
Dry: concentrated solution to be carried out spraying dry, obtains monascus yellow pigment powder.
Embodiment 4
A preparation method for monascus yellow pigment, step is as follows:
1) screening of monascus strain
Monascus anka Nakazawa et sato spore suspension prepare: by monascus strain (monascus (
monascus anka)be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 30th, 2015, deposit number is CGMCC NO.10909; ) blow into granulated glass sphere sterilized water, put shaking table 200r/min, jolting 1h.
Mutagenic treatment: the household microwave oven adopting peak power 1000w, pulse-repetition 2540MHZ, mid power intensity, according to 20-60s different time, carries out microwave treatment to Monascus anka Nakazawa et sato spore suspension, and the bacteria suspension drawing 0.1mL is coated on flat board.Subsequently flat board is placed in 15W ultraviolet lamp 30cm place and irradiates 15s, wrap up in black paper bag immediately and put 33 DEG C of constant incubators cultivation 7d.Select and produce the dark and fast strain excellent of yellow pigment color.
The genetic stability test of mutant strain: the dominant strain continuous passage filtered out cultivates 8 times, observes the ability and speed of producing yellow pigment.
2) extraction of monascus yellow pigment
Cultivate: the mutant strain of high yield yellow pigment being inoculated into becoming after sterilizing is in the slant medium of 4% maltose, 4% Zulkovsky starch, 3% peptone, 2% agar, is cultivate 10d in the shaking table of 33 DEG C in temperature
Fermentation: cultured excellent monascus specie being inoculated into composition is 6% Semen Maydis powder, 3% glucose, 0.3%(NH
4)
2sO
4, 0.1KH
2pO
4, 0.1MgSO
4fermention medium, carry out shake-flask culture 5d, culture temperature about 33 DEG C, adds 0.4% glucose solution at 3-5d every day, after fermentation fermented liquid
Filter: add hydrochloric acid and regulate fermented liquid pH3, filter after precipitation 6h, obtain filter residue;
Lixiviate: join in 80% ethanolic soln of 10 times of weight by filtration gained filter residue, stir 1-2h, adopt NaOH regulator solution pH to be 12, after soaking 9h, ceramic filtration membrane is filtered, and obtains vat liquor;
Concentrated: to adopt triple effect multi-step evaporator to concentrate vat liquor, obtain concentrated solution, with salt acid for adjusting pH 7;
Dry: concentrated solution to be carried out spraying dry, obtains monascus yellow pigment powder.
Spectrophotometry look valency is utilized to the present invention's example 1-4 monascus yellow pigment; Utilize high effective liquid chromatography for measuring citrinin content; By pigmentolysis under comparable sodium, Heating temperature to 100 DEG C, heating 5h, measures thermotolerance; By pigmentolysis under comparable sodium, under being placed on 40W ultraviolet lamp, illumination 5h, measures photostabilization.Concrete testing data is in table 1.
Table 1 monascus yellow pigment testing index
From table 1 data, the product monascus yellow pigment bacterial classification obtained duplex mutagenesis carries out the monascus yellow pigment of liquid submerged fermentation acquisition, look valency high (>=300U/g), citrinin content low (≤50U/Kg), photostabilization are strong, not fugitive color is a kind of monascus yellow pigment with broad mass market prospect.
Claims (10)
1. a monascus strain (
monascus anka), be deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 30th, 2015, deposit number is CGMCC NO.10909.
2. a preparation method for monascus yellow pigment, comprises following step:
(1) strain improvement
Select by mutagenesis be separated obtain monascus strain (
monascus anka), select and produce the strong and fast strain excellent of yellow pigment ability, described monascus strain is deposited in China Committee for Culture Collection of Microorganisms's common micro-organisms center on June 30th, 2015, and deposit number is CGMCC NO.10909;
(2) seed culture
By in the slant medium of the inoculation chosen in step (1) after sterilizing, at 30-37 DEG C, in shaking table, cultivate 8-12d;
(3) ferment: be inoculated in following fermention medium by excellent monascus specie cultured in step (2), described cultivation is specially: 4-8% Semen Maydis powder, 1-5% glucose, 0.1-0.5%(NH
4)
2sO
4, 0.05-0.15KH
2pO
4, 0.05-0.15MgSO
4, shake-flask culture 4-6d, culture temperature 30-37 DEG C, add 0.3-0.5% glucose solution at 3-5d every day, obtains fermented liquid after fermentation;
(4) filter: add hydrochloric acid and regulate fermented liquid pH2-3, filter after precipitation 4-8h, obtain filter residue;
(5) lixiviate: join in the 60%-80% ethanolic soln of 5-12 times of weight by filtration gained filter residue, stir 1-2h, adopt NaOH regulator solution pH to be 8-13, after soaking 5-12h, ceramic filtration membrane is filtered, and obtains vat liquor;
(6) concentrated: to adopt triple effect multi-step evaporator to concentrate vat liquor, obtain concentrated solution, with salt acid for adjusting pH about 7;
(7) dry: concentrated solution to be carried out spraying dry, obtains monascus yellow pigment powder.
3. the preparation method of a kind of monascus yellow pigment as claimed in claim 2, is characterized in that: the mutagenesis method in described step (1) is physics or ultraviolet mutagenesis.
4. the preparation method of a kind of monascus yellow pigment as claimed in claim 3, it is characterized in that: the mutagenesis method in described step (1) is physical mutagenesis, described physical mutagenesis is treated to and adopts peak power 1000w, the household microwave oven of pulse-repetition 2540MHZ, according to 20-60s different time, microwave treatment is carried out to Monascus anka Nakazawa et sato spore suspension, the bacteria suspension drawing 0.1mL is subsequently coated on flat board, subsequently flat board is placed in 15W ultraviolet lamp 30cm place and irradiates 10-30s, immediately wrap up in black paper bag and put 33 DEG C of constant incubators cultivation 7d, select and produce the strong and fast strain excellent of yellow pigment ability.
5. the preparation method of a kind of monascus yellow pigment as claimed in claim 4, is characterized in that, in described step (2),
In shaking table, 10d is cultivated at 33 DEG C.
6. the preparation method of a kind of monascus yellow pigment as claimed in claim 5, is characterized in that, in described step (3), substratum is specially: 6% Semen Maydis powder, 3% glucose, 0.3%(NH
4)
2sO
4, 0.1KH
2pO
4, 0.1MgSO
4, shake-flask culture 5d, culture temperature 30-37 DEG C, add 0.4% glucose solution at 3-5d every day, obtains fermented liquid after fermentation.
7. the preparation method of a kind of monascus yellow pigment as claimed in claim 6, is characterized in that, in described step (4), adds hydrochloric acid and regulates fermented liquid pH2.5, filter, obtain filter residue after precipitation 6h.
8. the preparation method of a kind of monascus yellow pigment as claimed in claim 7, it is characterized in that, in described step (5): filtration gained filter residue is joined in 70% ethanolic soln of 8 times of weight, stir 1.5h, NaOH regulator solution pH is adopted to be 10, after soaking 8h, ceramic filtration membrane is filtered, and obtains vat liquor.
9. the preparation method of a kind of monascus yellow pigment as claimed in claim 8, it is characterized in that, after step (1) selects bacterial strain, then carry out genetic stability test, be specially: the dominant strain continuous passage filtered out cultivates 8 times, select to produce the strong and fireballing bacterial strain of the ability of yellow pigment.
10. the preparation method of a kind of monascus yellow pigment as claimed in claim 2, is characterized in that: the mutagenesis method in described step (1) is ultraviolet mutagenesis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510441912.7A CN105018353A (en) | 2015-07-24 | 2015-07-24 | Preparation method for monascus yellow pigment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510441912.7A CN105018353A (en) | 2015-07-24 | 2015-07-24 | Preparation method for monascus yellow pigment |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105018353A true CN105018353A (en) | 2015-11-04 |
Family
ID=54408658
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510441912.7A Pending CN105018353A (en) | 2015-07-24 | 2015-07-24 | Preparation method for monascus yellow pigment |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105018353A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105558764A (en) * | 2015-12-09 | 2016-05-11 | 中南林业科技大学 | Monascus yellow pigment with high light stability |
| CN106967082A (en) * | 2017-04-05 | 2017-07-21 | 江苏鸣生物股份有限公司 | A kind of method for extracting monascus yellow pigment |
| CN110734940A (en) * | 2019-10-16 | 2020-01-31 | 武汉工程大学 | Preparation method of high-salt fermented monascus yellow pigment |
| CN112430635A (en) * | 2020-12-01 | 2021-03-02 | 广东肇庆星湖生物科技股份有限公司 | Fermentation method for low-yield citrinin and high-yield water-soluble monascus yellow pigment |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101649296A (en) * | 2009-09-23 | 2010-02-17 | 山东中惠食品有限公司 | Novel monascus strain and monascus corn food prepared from same |
| CN101914453A (en) * | 2010-07-14 | 2010-12-15 | 华南理工大学 | Monascus anka, and solid-state fermentation color-production method and application thereof |
| CN101942397A (en) * | 2010-09-13 | 2011-01-12 | 东莞市天益生物工程有限公司 | Monascus mutant strain and method for preparing monascus yellow pigment by submerged fermentation thereof and article thereof |
-
2015
- 2015-07-24 CN CN201510441912.7A patent/CN105018353A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101649296A (en) * | 2009-09-23 | 2010-02-17 | 山东中惠食品有限公司 | Novel monascus strain and monascus corn food prepared from same |
| CN101914453A (en) * | 2010-07-14 | 2010-12-15 | 华南理工大学 | Monascus anka, and solid-state fermentation color-production method and application thereof |
| CN101942397A (en) * | 2010-09-13 | 2011-01-12 | 东莞市天益生物工程有限公司 | Monascus mutant strain and method for preparing monascus yellow pigment by submerged fermentation thereof and article thereof |
Non-Patent Citations (3)
| Title |
|---|
| 彭真等: "红曲黄色素固态发酵条件的研究", 《现代食品科技》 * |
| 田园等: "氨基酸对红曲霉突变菌株合成代谢黄色素和橘霉素的影响", 《中国酿造》 * |
| 程鹏飞等: "红曲霉(Monascus anka As 3.782)菌种的紫外诱变和产色素的条件优化", 《食品研究与开发》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105558764A (en) * | 2015-12-09 | 2016-05-11 | 中南林业科技大学 | Monascus yellow pigment with high light stability |
| CN106967082A (en) * | 2017-04-05 | 2017-07-21 | 江苏鸣生物股份有限公司 | A kind of method for extracting monascus yellow pigment |
| CN106967082B (en) * | 2017-04-05 | 2018-12-04 | 江苏一鸣生物股份有限公司 | A method of extraction monascus yellow pigment |
| CN110734940A (en) * | 2019-10-16 | 2020-01-31 | 武汉工程大学 | Preparation method of high-salt fermented monascus yellow pigment |
| CN110734940B (en) * | 2019-10-16 | 2021-08-24 | 武汉工程大学 | Preparation method of high-salt fermented monascus yellow pigment |
| CN112430635A (en) * | 2020-12-01 | 2021-03-02 | 广东肇庆星湖生物科技股份有限公司 | Fermentation method for low-yield citrinin and high-yield water-soluble monascus yellow pigment |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106222098B (en) | One plant of monascus strain and its application | |
| CN101235353B (en) | Monascus mutant and method for preparing flavochrome by fermenting the same | |
| CN104893983B (en) | Liquid state fermentation low citrinin, the preparation method of High color values monascorubin and product | |
| CN105861401B (en) | One plant of Xanthomonas campestris NYW79 and application thereof | |
| CN102178035B (en) | Method for fermenting and decomposing gossypol in cottonseed meal by composite strains | |
| CN105018353A (en) | Preparation method for monascus yellow pigment | |
| CN102653724B (en) | Lactobacillus casei and application thereof to produce L-lactic acid by fermentation | |
| CN104498528A (en) | Monascus fermentation method for low-yield citrinin and high-yield monascorubin | |
| CN104178430B (en) | Astaxanthin high-yield strain and application thereof | |
| CN101942397A (en) | Monascus mutant strain and method for preparing monascus yellow pigment by submerged fermentation thereof and article thereof | |
| CN105861342A (en) | Phaffia rhodozyma strain rich in astaxanthin and screening method and application of Phaffia rhodozyma strain | |
| CN100494390C (en) | Method for preparing low citrinin monascus pigment from rice | |
| CN108841731A (en) | The Monascus Strains of high yield pigment and its application | |
| CN107188634A (en) | A kind of production method of selenium-enriched high-calcium large-fruited Chinese hawthorn | |
| CN106520563B (en) | A kind of acid-resistant alpha-amylase bacterial strain and its production method | |
| CN100516143C (en) | Process of producing gardenia blue pigment | |
| CN103451122A (en) | Bacillus subtilis capable of massively yielding haematochrome and application thereof | |
| CN103194500A (en) | Fermentation preparation method of epsilon-poly-L-lysine | |
| CN103564194B (en) | Cordyceps polysaccharide composition, as well as preparation method and application of cordyceps polysaccharide composition | |
| CN102154078B (en) | Preparation method of nutrient solution required by cultivating strong aromatic Chinese spirits pit mud | |
| CN104711208B (en) | A kind of lactic acid bacteria with high starch capacity of decomposition | |
| CN105695514B (en) | Red rice red yeast and preparation method and application thereof | |
| CN104694607B (en) | A kind of method that solid state fermentation prepares beta carotene | |
| CN104045461B (en) | A kind of Cordyccps-militaris-(L.)-link. Sporophore substratum adding Flower of Arabian Jasmine slag | |
| CN108339502B (en) | Preparation method of monascus pigment microcapsules |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151104 |
|
| RJ01 | Rejection of invention patent application after publication |