CN106967082A - A kind of method for extracting monascus yellow pigment - Google Patents
A kind of method for extracting monascus yellow pigment Download PDFInfo
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- CN106967082A CN106967082A CN201710216652.2A CN201710216652A CN106967082A CN 106967082 A CN106967082 A CN 106967082A CN 201710216652 A CN201710216652 A CN 201710216652A CN 106967082 A CN106967082 A CN 106967082A
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- yellow pigment
- monascus yellow
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- C07—ORGANIC CHEMISTRY
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- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
- C07D493/04—Ortho-condensed systems
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Abstract
The invention discloses a kind of method for extracting monascus yellow pigment, belong to field of fermentation engineering.Concentration, use ratio, extraction temperature, extraction time, extraction times of the invention by adjusting extractant methanol/ethanol solution, to reach that extractant and lipid solvent are immiscible, and the purpose for effectively extracting monascus yellow pigment, so as to be conducive to the further concentration of fat-soluble pigment to utilize.Using the easy quick, economical and efficient of the inventive method extraction monascus yellow pigment, and the structure of pigment will not be changed, be a kind of preferable method of purification, the feasibility in industrial mass production is higher.
Description
Technical field
The present invention relates to a kind of method for extracting monascus yellow pigment, natural red yeast rice is yellow especially in Fermentation Engineering downstream technique
The extracting method of pigment, belongs to Fermentation Engineering and biological technical field.
Background technology
Monascus yellow pigment is the natural pigment that Monascus is produced in growth metabolism process, and monascorubin is a class polyketide
Thing, first by basic component unit of acetyl-CoA, polymerization obtains the chromophore of pigment, and then chromophore exists with aliphatic acid again
Be polymerized different monascorubins in the presence of polymerase.Monascus yellow pigment molecule contains substantial amounts of ketone group and miscellaneous containing three
Ring, polarity is smaller, and monascus yellow pigment is soluble in glycerides, is not readily dissolved in water.
A kind of patent of invention " biliquid of coupling situ extracting fermentation monascus yellow pigment of Application No. 201410116854.6
In phase fermentation method ", pass through the content and Two Liquid Phases of free amino acid in carbon nitrogen source component reasonable in design, control culture medium
Fermentation system, the monascus yellow pigment of synthesis is enriched in fat-soluble solvent rapidly, so as to improve the production effect of monascus yellow pigment
Rate.Monascus yellow pigment color value can reach more than 500U/mL in 1000L mechanical agitating fermentation tanks at present.But utilize coupling former
During the biliquid phase fermentation method production uranidin of position extraction, pigment is mainly dissolved in extraction phase glycerides, and glycerine
The volatility of Ester is very low and can not be miscible with water, and therefore, it is difficult to utilize.How by these pigments from lipid solvent
It is transferred in the stronger solvent of volatility, and the pigment sample concentrated by using rotary evaporation technique solvent flashing, carry
High pigment yield is the significant problem for needing to solve in engineering.And dissolving of many pigments in glycerides in red yeast rice
Degree is generally higher than the solubility in other solvents, therefore, pigment is transferred out from glycerides, as problem.
It is that uranidin is enriched in lipid solvent using the difficult point of the fat-soluble uranidin of solvent extraction, and not normal
In the zymotic fluid (water) of rule.Conventional solvent selection is difficult to reach good effect of extracting, and extraction of such as chloroform to pigment is imitated
Fruit is preferable, but chloroform can dissolve each other with lipid solvent, therefore is unable to reach the experiment purpose of layering extraction；And formic acid etc. and lipid
Solvent is simultaneously immiscible, but their solubility to pigment are smaller, are equally unable to reach preferable effect of extracting.
The content of the invention
The problem to be solved in the present invention is to extract the fat-soluble monascus yellow pigment being dissolved in lipid solvent, to prepare
The higher pigment concentrate of concentration creates conditions.
Therefore, of the invention, by volumetric concentration for 30% to 90% methanol or ethanol water with containing monascus yellow pigment
Lipid solvent is mixed with volume ratio 2~4, and 10min~30min is extracted under conditions of 25 DEG C -50 DEG C, is extracted 1~5 time, red yeast rice
Uranidin is transferred to extraction phase, obtains monascus yellow pigment extract.
By resulting monascus yellow pigment extract rotary evaporation, methanol or ethanol are removed, highly concentrated pigment is obtained
Solution.
The lipid solvent refers to glycerides, such as triglyceride material, di-glycerides material, glycerine list
Ester or its mixture.The triglyceride material include tricaprylin, three isooctyl acid glyceride, vegetable oil or
Its mixture.The di-glycerides material is 1,3- diglycerides, 1,2- diglycerides, two glycerol caprylates or its mixing
Thing.The monoglyceride class material is Capmul MCM C8.
In one embodiment of the invention, by volumetric concentration for 70% to 85% methanol or ethanol water with containing
The lipid solvent of monascus yellow pigment is mixed with volume ratio 2~4, and 10min~30min, extraction are extracted under conditions of 25 DEG C -40 DEG C
1~3 time, monascus yellow pigment is transferred to extraction phase, obtains monascus yellow pigment extract.
In one embodiment of the invention, by volumetric concentration for 85% ethanol water with containing monascus yellow pigment
Lipid solvent is mixed with volume ratio 4, and 10min~30min is extracted under conditions of 25 DEG C -40 DEG C, is extracted 1~3 time, red yeast rice yellow
Element is transferred to extraction phase, obtains monascus yellow pigment extract.
Concentration, use ratio, extraction temperature, extraction time, extraction of the invention by adjusting extractant methanol/ethanol solution
Number of times is taken, to reach that extractant and lipid solvent are immiscible, and the purpose for effectively extracting monascus yellow pigment, so as to have
Further concentration beneficial to fat-soluble pigment is utilized.Using the easy quick, economical and efficient of the inventive method extraction monascus yellow pigment,
And the structure of pigment will not be changed, it is a kind of preferable method of purification, the feasibility in industrial mass production is higher.
Brief description of the drawings
Monascus yellow pigment full wavelength scanner figure in Fig. 1 zymotic fluids
Fig. 2 extracted through ethanol after monascus yellow pigment full wavelength scanner figure
Embodiment
(1) assay method of monascus yellow pigment color value：
Color value is a kind of method for expressing of pigment concentration, and size is equal to extension rate and inhaled with the pigment solution after dilution at it
The product of corresponding OD values at peak is received, unit is U/mL.
Solution to be measured is diluted into suitable multiple (by the control of OD values between 0.2-0.8) with absolute ethyl alcohol, and with anhydrous second
The absorbance of alcohol is that blank control carries out full wavelength scanner in the range of 200-600nm, determines the OD of peak value between 400-440nm
Value.
Monascus yellow pigment color value=OD410nm× total extension rate (U/mL)
(2) pigment rate of transform computational methods：
The pigment rate of transform=(extraction phase color value (U/mL) × extraction phase volume (mL))/(fat-soluble solvent is total before extraction
The cumulative volume (mL) of fat-soluble solvent before color value (U/mL) × extraction) × 100%
Embodiment 1
The volumetric concentration of 2 times of volumes of addition is in the fat-soluble solvent for being 631U/mL to initial monascus yellow pigment color value
70% butanol solution, 40 DEG C of extraction temperature, progress single extraction, extraction time 20min, lamination substantially, extracts after extraction
Take rear fat-soluble solvent phase color value 561.71U/mL.Wherein the pigment rate of transform is 10.98%, and butanol phase rotary evaporation is obtained into height
Concentration samples, its color value is 1657.89U/mL.
Embodiment 2
The volume ratio that 2 times of volumes are added in the fat-soluble solvent for being 631U/mL to initial color value is 1:1 methanol/ethanol
Solution, 40 DEG C of extraction temperature, progress single extraction, extraction time 20min, lamination is substantially, fat-soluble after extraction after extraction
Solvent phase phase color value is 551.23U/mL.I.e. wherein the pigment rate of transform is 12.64%, and ethanol phase rotary evaporation is obtained into high concentration
Sample, its color value is 2456.21U/mL.
Embodiment 3
The volumetric concentration of 2 times of volumes of addition is in the fat-soluble solvent for being 631U/mL to initial monascus yellow pigment color value
70% ethanol solution, 40 DEG C of extraction temperature, progress single extraction, extraction time 20min, lamination is obvious after extraction, fat
Remaining color value is that 402.32U/mL, i.e. the pigment rate of transform are 23.51% in soluble solvent phase, and ethanol phase rotary evaporation is obtained into height
Concentration samples, its color value is 3665.24U/mL.
Embodiment 4
The methanol that the volumetric concentration that 2 times of volumes are added in the fat-soluble solvent for being 631U/mL to initial color value is 70% is molten
Liquid, 40 DEG C of extraction temperature, progress single extraction, extraction time 20min, lamination is substantially, fat-soluble molten after extraction after extraction
Agent phase phase color value is 451.54U/mL.I.e. wherein the pigment rate of transform is 16.87%, and ethanol phase rotary evaporation is obtained into high concentration sample
Product, its color value is 2954.36U/mL.
Embodiment 5
The ethanol that the volumetric concentration that 3 times of volumes are added in the fat-soluble solvent for being 631U/mL to initial color value is 70% is molten
Liquid, 40 DEG C of extraction temperature, progress single extraction, extraction time 20min, lamination is substantially, fat-soluble molten after extraction after extraction
Agent phase phase color value is 358.74U/mL.I.e. wherein the pigment rate of transform is 34.49%, and ethanol phase rotary evaporation is obtained into high concentration sample
Product, its color value is 5142.61U/mL.
Embodiment 6
The ethanol that the volumetric concentration that 4 times of volumes are added in the fat-soluble solvent for being 631U/mL to initial color value is 70% is molten
Liquid, 40 DEG C of extraction temperature, progress single extraction, extraction time 20min, lamination is substantially, fat-soluble molten after extraction after extraction
Agent phase phase color value is 360.15U/mL.I.e. wherein the pigment rate of transform is 30.50%, and ethanol phase rotary evaporation is obtained into high concentration sample
Product, its color value is 4562.33U/mL.
Embodiment 7
The ethanol that the volumetric concentration that 4 times of volumes are added in the fat-soluble solvent for being 631U/mL to initial color value is 90% is molten
Liquid, 40 DEG C of extraction temperature, progress single extraction, extraction time 20min, lamination is substantially, that is, fat-soluble after extracting after extraction
Solvent phase phase color value is 360.54U/mL.Wherein the pigment rate of transform is 34.97%, and ethanol phase rotary evaporation is obtained into high concentration sample
Product, its color value is 5214.62U/mL.
Embodiment 8
The ethanol that the volumetric concentration that 4 times of volumes are added in the fat-soluble solvent for being 631U/mL to initial color value is 85% is molten
Liquid, 40 DEG C of extraction temperature, progress single extraction, extraction time 20min, lamination is substantially, that is, fat-soluble after extracting after extraction
Solvent phase phase color value is 340.24U/mL.Wherein the pigment rate of transform is 35.86%, and ethanol phase rotary evaporation is obtained into high concentration sample
Product, its color value is 5631.58U/mL.
Embodiment 9
The ethanol that the volumetric concentration that 4 times of volumes are added in the fat-soluble solvent for being 631U/mL to initial color value is 85% is molten
Liquid, 50 DEG C of extraction temperature, progress single extraction, extraction time 20min, lamination is substantially, that is, fat-soluble after extracting after extraction
Solvent phase phase color value is 450.78U/mL.Wherein the pigment rate of transform is 31.06%, and ethanol phase rotary evaporation is obtained into high concentration sample
Product, its color value is 4985.29U/mL.
Embodiment 10
The ethanol that the volumetric concentration that 4 times of volumes are added in the fat-soluble solvent for being 631U/mL to initial color value is 85% is molten
Liquid, 25 DEG C of extraction temperature, progress single extraction, extraction time 20min, lamination is substantially, that is, fat-soluble after extracting after extraction
Solvent phase phase color value is 330.27U/mL.Wherein the pigment rate of transform is 36.54%, and ethanol phase rotary evaporation is obtained into high concentration sample
Product, its color value is 5784.72U/mL.
Embodiment 11
The ethanol that the volumetric concentration that 4 times of volumes are added in the fat-soluble solvent for being 631U/mL to initial color value is 85% is molten
Liquid, 25 DEG C of extraction temperature, progress single extraction, extraction time 30min, lamination is substantially, that is, fat-soluble after extracting after extraction
Solvent phase phase color value is 342.26U/mL.Wherein the pigment rate of transform is 33.87%, and ethanol phase rotary evaporation is obtained into high concentration sample
Product, its color value is 5421.64U/mL.
Embodiment 12
The ethanol that the volumetric concentration that 4 times of volumes are added in the fat-soluble solvent for being 631U/mL to initial color value is 85% is molten
Liquid, 25 DEG C of extraction temperature, progress single extraction, extraction time 10min, lamination is substantially, that is, fat-soluble after extracting after extraction
Solvent phase phase color value is 322.22U/mL.Wherein the pigment rate of transform is 37.22%, and ethanol phase rotary evaporation is obtained into high concentration sample
Product, its color value is 6102.85U/mL.
Embodiment 13
The ethanol that the volumetric concentration that 4 times of volumes are added in the fat-soluble solvent for being 631U/mL to initial color value is 85% is molten
Liquid, 25 DEG C of extraction temperature carries out three times and extracted, each extraction time 10min, and lamination is obvious after final extraction, after extraction
Fat-soluble solvent phase phase color value is 80.41U/mL.I.e. wherein the pigment rate of transform is 61.1%, and ethanol phase rotary evaporation is obtained into height
Concentration samples, its color value is 6305.12U/mL.
Embodiment 14
The ethanol that the volumetric concentration that 4 times of volumes are added in the fat-soluble solvent for being 631U/mL to initial color value is 85% is molten
Liquid, 25 DEG C of extraction temperature carries out five times and extracted, each extraction time 10min, and lamination is obvious after final extraction, after extraction
Fat-soluble solvent phase phase color value is 15.22U/mL.I.e. wherein the pigment rate of transform is 83.5%, and ethanol phase rotary evaporation is obtained into height
Concentration samples, its color value is 6631.85U/mL.
It can be seen that according to Fig. 1 and Fig. 2 and fat-soluble pigment be extracted into alcohols material, its wave-length coverage still exists
In the range of 401nm-420nm, it is still uranidin to illustrate it.
Although the present invention is disclosed as above with preferred embodiment, it is not limited to the present invention, any to be familiar with this skill
The people of art, without departing from the spirit and scope of the present invention, can do various changes and modification, therefore the protection model of the present invention
Enclose being defined of being defined by claims.
Claims (6)
1. in a kind of solvent from lipid extract monascus yellow pigment method, it is characterised in that by volumetric concentration be 30% to 90%
Methanol or ethanol water mixed with the lipid solvent containing monascus yellow pigment with volume ratio 2~4, in 25 DEG C -50 DEG C of condition
Lower extraction 10min~30min, is extracted 1~5 time, monascus yellow pigment is transferred to extraction phase, obtains monascus yellow pigment extract.
2. the method for monascus yellow pigment is extracted in a kind of solvent from lipid according to claim 1, it is characterised in that described
Lipid solvent refers to glycerides, including triglyceride material, di-glycerides material, monoglyceride class material or its
Mixture.
3. the method for monascus yellow pigment is extracted in a kind of solvent from lipid according to claim 1 or 2, it is characterised in that
By volumetric concentration for 70% to 85% methanol or ethanol water and the lipid solvent containing monascus yellow pigment with volume ratio 2~4
Mixing, extracts 10min~30min under conditions of 25 DEG C -40 DEG C, extracts 1~3 time, monascus yellow pigment is transferred to extraction phase, obtains
To monascus yellow pigment extract.
4. the method for monascus yellow pigment is extracted in a kind of solvent from lipid according to claim 1 or 2, it is characterised in that
Volumetric concentration is mixed for 85% ethanol water with the lipid solvent containing monascus yellow pigment with volume ratio 4, at 25 DEG C -40 DEG C
Under conditions of extract 10min~30min, extract 1~3 time, monascus yellow pigment is transferred to extraction phase, obtain monascus yellow pigment extraction
Liquid.
5. the method for monascus yellow pigment is extracted in a kind of solvent from lipid according to claim 4, it is characterised in that by body
Product concentration is mixed for 85% ethanol water with the lipid solvent containing monascus yellow pigment with volume ratio 4, under conditions of 25 DEG C
10min is extracted, is extracted 1 time, monascus yellow pigment is transferred to extraction phase, obtains monascus yellow pigment extract.
6. according to the method that monascus yellow pigment is extracted in a kind of any described solvent from lipid of Claims 1 to 5, its feature exists
In by resulting monascus yellow pigment extract rotary evaporation, removal methanol or ethanol obtain highly concentrated pigment solution.
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Cited By (2)
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| CN110652007A (en) * | 2019-11-06 | 2020-01-07 | 满山歌茶业(西双版纳)有限公司 | Method for preparing theaflavin |
| CN111248361A (en) * | 2020-02-05 | 2020-06-09 | 播恩生物技术股份有限公司 | Laying hen feed capable of increasing egg yolk degree and realizing accurate batching |
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