CN108004195A - A kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract - Google Patents
A kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract Download PDFInfo
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- CN108004195A CN108004195A CN201711478930.8A CN201711478930A CN108004195A CN 108004195 A CN108004195 A CN 108004195A CN 201711478930 A CN201711478930 A CN 201711478930A CN 108004195 A CN108004195 A CN 108004195A
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Abstract
The invention discloses a kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract, detailed process are as follows：The Chinese sumac leaf extract that extracting concentration is 0.015~0.05g/L is added in the chlorella after culture.The present invention promotes rapid growth of chlorella using Chinese sumac leaf, and process is simple, derives from a wealth of sources, and cost is low, environmentally protective, and preparation effect is good, makees raw material using energy forest residue, opens the new way that a kind of forest tree remainder utilizes；New technical thought is provided for industrialization rapid, high volume culture chlorella.
Description
Technical field
The present invention relates to a kind of method for promoting rapid growth of chlorella, more particularly to one kind to be carried using Chinese sumac leaf
Thing is taken to promote the method for rapid growth of chlorella.
Background technology
Chlorella has the characteristics that photoautotrophic cultivation, breeding fast and stable, cultivating system are not take up arable land, it is distributed wide
It is general, be easy to cultivate, can in efficient bioreactor mass propgation, be especially suitable for industrial large-scale production.Bead at present
Algae has had been reported for production biodiesel, unrighted acid, vitamin, and secondary metabolites carotenoid, astaxanthin
With amino acid etc..Particularly from chlorella bioactive substance extracting solution mutually compounded with ordinary nutritional fertilizer after exploitation into high attached
The biological medicine fertilizer of value, is untapped huge resources bank, has potential produce reality application value.In addition the life of chlorella
Thing active extract contains various active amino acid and a large amount of secondary metabolites, can be as the organic nutrition feed of animal
And fish food additive, there is the immunity for improving animals and plants, for developing the costly agriculture and animal husbandry fishing of green organic nutrition
Industry product, has huge development prospect.However, the traditional training method of chlorella is as mainly photosynthetic by the use of organic carbon source
Act on raw material accumulation metabolin.When using photosynthetic raw material of the exogenous irritant as chlorella, its growth speed can be improved simultaneously
And a large amount of biological metabolites are effectively accumulated, so as to reach the algae density and biomass of superelevation, efficient productivity will be
To a certain extent offset chlorella it is photosynthetic cultivation culture need organic carbon source cost, be follow-up chlorella High Density Cultivation and
The exploitation of high added value metabolite provides Research foundation.But chlorella cultivation cycle is grown at present, it is impossible to a large amount of industry metaplasias
Production.
The content of the invention
It is an object of the invention to overcome the deficiencies of the prior art and provide a kind of small using the promotion of Chinese sumac leaf extract
The method of ball algae fast-growth, promotes rapid growth of chlorella using the crude extract of woods source leaves of plants.
The present invention is achieved by the following technical solutions：Detailed process is as follows：It is 0.015~0.05g/ by extracting concentration
The Chinese sumac leaf extract of L is added in the chlorella after culture.
The preparation method of the Chinese sumac leaf extract is as follows：
(1) Chinese sumac leaf is broken into single-size, sieved；
(2) Chinese sumac blade powder and extracting solution are pressed 1：5~25 solid-liquid ratio progress hot dipping carries, ultrasonic wave assisted extraction
L~4 time, 1~4h processing is carried out under 40~80 DEG C of Extracting temperature to mixture, is filtered, and centrifugation, merges after collecting supernatant
Filtrate, removes hybrid particles material in extracting solution after being filtered under diminished pressure to filtrate, degerming to extracting solution centrifugal filtration to obtain salt
Skin wood leaf crude extract.
Mixing extraction, the assisted extraction liquid are carried out to Chinese sumac leaf using different auxiliary extracting solutions in the step (2)
For water, Extracting temperature is 40~60 DEG C, and the ultrasonic wave extraction of 1~2h of progress 1~3 time, the same terms are once extracted using with preceding
Once extracted after being carried out to the residue after preceding once extraction centrifugation, supernatant twice is merged after centrifugation and completes extraction, is subtracted after constant volume
Press filtration removes hybrid particles material in extracting solution, and extracting solution centrifugal filtration is degerming to obtain Chinese sumac leaf crude extract.
Mixing extraction, the assisted extraction are carried out to Chinese sumac leaf using different assisted extraction methods in the step (2)
Liquid is water, and Extracting temperature is 60~80 DEG C, and the hot dipping for carrying out 3~4h carries 2~4 times, is filtered under diminished pressure and is removed in extracting solution after constant volume
Extracting solution, is carried out that centrifugal filtration is degerming obtains Chinese sumac leaf crude extract by hybrid particles material.
Mixing extraction, the assisted extraction liquid are carried out to Chinese sumac leaf using different auxiliary extracting solutions in the step (2)
For ethanol, Extracting temperature is 50~70 DEG C, and the hot dipping for carrying out 1~3h carries 1~2 time, is filtered under diminished pressure and is removed in extracting solution after constant volume
Hybrid particles material, extracting solution centrifugal filtration is degerming to obtain Chinese sumac leaf crude extract.
Mixing extraction, the assisted extraction liquid are carried out to Chinese sumac leaf using different auxiliary extracting solutions in the step (2)
For ethanol, Extracting temperature is 40~70 DEG C, and the ultrasonic wave extraction of every 2~4h 2~4 times, is filtered under diminished pressure removing extracting solution after constant volume
Middle hybrid particles material, extracting solution centrifugal filtration is degerming to obtain Chinese sumac leaf crude extract.
Supersonic extracting method used can control extraction time.The recovery rate of polyphenol is related with extraction time, during extraction
Between the long polyphenol that can cause in extracting solution the chemical reactions such as oxidation, degraded occur, reduce recovery rate on the contrary.
When assisted extraction liquid is ethanol, the volumetric concentration of ethanol is 60%.When volume fraction of ethanol is relatively low, mixed liquor
Water-solubility protein, the carbohydrate of middle dissolving are more；And when volume fraction of ethanol is higher, liposoluble substance dissolving is more, all can shadow
Ring the recovery rate of polyphenol in mixture.
Chlorella condition of culture is：Using the BG11 culture mediums Jing Guo filtration sterilization in the triangle by autoclave sterilization
It is inoculated with and cultivates in flask, the chlorella being inoculated with is placed in artificial climate incubator in culture, and temperature is 23 ± 1 DEG C, illumination
Intensity is 2000~6000lx, Light To Dark Ratio 12h：12h, inoculation in every 12~16 days once, chlorella are uniformly distributed to obtain
Enough illumination.
Chinese sumac Leave extract contains the natural flavonoid compound of multiple phenolic hydroxyl groups, passes through certain external model of oxidation
It is verified except having the function that in anti-oxidant scavenger-cell in addition to free radical activity, the Flavonoid substances of most of plant origin
A kind of natural plants stimulin can be used as, influences the biochemical metabolism reaction process of frustule.Flavonoid substances can be used as biology
Stimulin, promotes the accumulation of acetylcholine in frustule, influences absorption of the inoculationin vitro to oxygen, suppress the chloroplaset of light stimulus
Expansion；Sodium ion and potassium ion is stimulated to be flowed out from chloroplaset, permeability and electron transmission and ATP of the regulation and control chloroplast membranes to ion
Coupling between synthesis.In addition phosphatidyl-ethanolamine and phosphatidyl choline metabolic pathway in regulation and control frustule, regulation and control be may also participate in
Cell membrane produces second messenger and opens calcium channel raising intracellular Ca to the permeability of ion2+Concentration, influences the various physiology of frustule
Process.The kinase expression that all kinds of dependence calcium ion regulatories can be activated at the same time improves, and activates the protein kinase C combined with plasma membrane,
Activating phosphataseization is reacted, and promotes frustule cell Proliferation and differentiation etc., so as to improve bead algae biomass.
The present invention has the following advantages compared with prior art：The present invention promotes rapid growth of chlorella using Chinese sumac leaf,
Process is simple, derives from a wealth of sources, and cost is low, environmentally protective, and preparation effect is good, makees raw material using energy forest residue, opens one
The new way of kind forest residue utilization；New technical thought is provided for industrialization rapid, high volume culture chlorella.
Brief description of the drawings
Fig. 1 is the growth contrast block diagram of chlorella；
Fig. 2 is the chlorella growth comparison diagram of embodiment 3 before and after the processing.
Embodiment
Elaborate below to the embodiment of the present invention, the present embodiment is carried out lower premised on technical solution of the present invention
Implement, give detailed embodiment and specific operating process, but protection scope of the present invention is not limited to following implementation
Example.
Embodiment 1
After gathering Chinese sumac blade, blade is placed in baking oven immediately, 5~10min of sterilization processing are carried out using 120 DEG C,
Blade is dried within 60 DEG C afterwards, then uniform leaf particles are broken into pulverizer, is screened to 30~100 mesh.
Chinese sumac leaf particles will be obtained is stored in brown closed container dry collection.
Weigh a certain amount of Chinese sumac leaf powder to be placed in the glass container of 1L, solution and Chinese sumac blade powder are pressed 1:
The material ratio of 15g/ml is uniformly mixed, and solution is water, and heating progress ultrasonic wave extraction after mixing, Extracting temperature is 80 DEG C, will be mixed
Close liquid and insert the ultrasonic wave extraction 2 times that Ultrasonic Cell Disruptor carries out 1h.First time is extracted using with first time extraction the same terms
Residue after centrifugation extract for the second time, and supernatant twice is merged after centrifugation and completes to extract for the second time, to the mixing after crushing
It is filtered under diminished pressure again after thing constant volume, removes hybrid particles material in extracting solution, extracting solution 5000rpm is centrifuged into 20min, then use filter membrane
Filtration sterilization, retains supernatant.A part of supernatant is taken to be freeze-dried up to Chinese sumac leaf extract dry powder.
The treatment group in the present embodiment, adding extract is denoted as CVW1.
Embodiment 2
After gathering Chinese sumac blade, blade is placed in baking oven immediately, 5~10min of sterilization processing are carried out using 120 DEG C,
Blade is dried within 60 DEG C afterwards, then uniform leaf particles are broken into pulverizer, is screened to 30~100 mesh.
Chinese sumac leaf particles will be obtained is stored in brown closed container dry collection.
Weigh a certain amount of Chinese sumac leaf powder to be placed in the glass container of 1L, assisted extraction liquid is pressed with Chinese sumac blade powder
1:15 ratio, assisted extraction liquid are water, and Extracting temperature is 80 DEG C, and the hot dipping for carrying out 2h carries 1 time.Removing is filtered under diminished pressure after constant volume
Hybrid particles material in extracting solution, finally carries out centrifugation 20min operations with 5000rpm by extracting solution, then degerming with membrane filtration
Obtain Chinese sumac leaf crude extract.A part of supernatant is taken to be freeze-dried up to Chinese sumac leaf extract dry powder.
The treatment group that extract is added in the present embodiment is denoted as CVW2.
Embodiment 3
The solid-liquid ratio of extraction solution and Chinese sumac blade powder is used as 1:15g/ml, assisted extraction liquid are 60% second
Alcohol, under 60 DEG C of Extracting temperature, the hot dipping for carrying out 2h carries 2 times.The solubility of polyphenols in ethanol can be with temperature
The rise of degree and increase, but when temperature is excessive, also results in polyphenol and the chemical reaction such as oxygenolysis occur.In view of phenolic material
The heat endurance of matter, solvent volatilize and the dissolution problem of impurity, therefore excellent to extraction conditions using 60 DEG C of methods repeatedly extracted
Change.Extracted using carrying out second to the residue after first time extraction centrifugation with first time extraction the same terms, merged after centrifugation
Supernatant completes second of extraction twice, is filtered under diminished pressure after constant volume and removes hybrid particles material in extracting solution, using 5000rpm pairs
Extracting solution is collected after centrifuging 20min into property, and membrane filtration is degerming, retains supernatant.A part of supernatant is taken to be freeze-dried up to salt skin
Wooden leaf ethanol extract dry powder.A part of supernatant is taken to be freeze-dried up to Chinese sumac leaf extract dry powder.
The treatment group that extract is added in the present embodiment is denoted as CVE1.
Embodiment 4
The solid-liquid ratio of extraction solution and Chinese sumac blade powder is used as 1:15g/ml, assisted extraction liquid are 60% second
Alcohol, under 60 DEG C of Extracting temperature, ultrasonic wave extraction of the progress per 1h 4 times.The same terms are extracted to first using with first time
Second of extraction of residue progress after secondary extraction centrifugation, merges supernatant twice and completes second of extraction after centrifugation, and so on.
It is filtered under diminished pressure after constant volume and removes hybrid particles material in extracting solution, is received after centrifuging 20min into property to extracting solution using 5000rpm
Collection, membrane filtration is degerming, retains supernatant.A part of supernatant is taken to be freeze-dried up to Chinese sumac leaf ethanol extract dry powder.Take one
Part supernatant is freeze-dried up to Chinese sumac leaf extract dry powder.
The treatment group that extract is added in the present embodiment is denoted as CVE2.Add the treatment group and blank control phase of extract
Than when the addition concentration of Chinese sumac is 0.025g/L, the 3rd day growth promotion rate to chlorella can reach common process group
2.34 again.
Chlorella made from embodiment 1~4 and blank control group contrast as shown in Figure 1：
By CVW1 compared with blank control Control, when the addition concentration of Chinese sumac is 0.015g/L, the 3rd day to small
The growth promotion rate of ball algae can reach 1.87 times of common process group.
CVW2 is compared with blank control Control, and when the addition concentration of Chinese sumac is 0.025g/L, the 3rd day to bead
The growth promotion rate of algae can reach 1.98 times of common process group.
CVE1 is compared with blank control Control, and when the addition concentration of Chinese sumac is 0.035g/L, the 3rd day to bead
The growth promotion rate of algae can reach 2.34 times of common process group.
CVE2 is compared with blank control, when the addition concentration of Chinese sumac is 0.05g/L, the growth to chlorella in the 3rd day
Promotion rate can reach 2.18 times of common process group.
As shown in Fig. 2, embodiment 3 handle after chlorella be the right in figure photo, the left side is the photo of before processing in figure,
It can be seen from the figure that chlorella growth is quickly, color is substantially deepened in whole container.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should all be included in the protection scope of the present invention.
Claims (9)
- A kind of 1. method for promoting rapid growth of chlorella using Chinese sumac leaf extract, it is characterised in that detailed process is as follows： The Chinese sumac leaf extract that extracting concentration is 0.015~0.05g/L is added in the chlorella after culture.
- 2. a kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract according to claim 1, it is special Sign is that the preparation method of the Chinese sumac leaf extract is as follows：(1) Chinese sumac leaf is broken into single-size, sieved；(2) Chinese sumac blade powder and extracting solution are pressed 1：5~25 solid-liquid ratio progress hot dipping carries, ultrasonic wave assisted extraction l~4 It is secondary, 1~4h processing is carried out to mixture under 40~80 DEG C of Extracting temperature, is filtered, centrifugation, merges filter after collecting supernatant Liquid, removes hybrid particles material in extracting solution after being filtered under diminished pressure to filtrate, degerming to extracting solution centrifugal filtration to obtain salt skin Wooden leaf crude extract.
- 3. a kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract according to claim 2, it is special Sign is, mixing extraction is carried out to Chinese sumac leaf using different auxiliary extracting solutions in the step (2), the assisted extraction liquid is Water, Extracting temperature are 40~60 DEG C, the ultrasonic wave extraction of 1~2h of progress 1~3 time, and the same terms pair are once extracted using with preceding Residue after preceding once extraction centrifugation once extracts after carrying out, and supernatant twice is merged after centrifugation and completes extraction, is depressurized after constant volume Hybrid particles material in extracting solution is filtered to remove, extracting solution centrifugal filtration is degerming to obtain Chinese sumac leaf crude extract.
- 4. a kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract according to claim 2, it is special Sign is, mixing extraction, the assisted extraction liquid are carried out to Chinese sumac leaf using different assisted extraction methods in the step (2) For water, Extracting temperature is 60~80 DEG C, and the hot dipping for carrying out 3~4h carries 2~4 times, is filtered under diminished pressure to remove in extracting solution after constant volume and mixes Particulate matter is closed, extracting solution carries out to centrifugal filtration is degerming obtains Chinese sumac leaf crude extract.
- 5. a kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract according to claim 2, it is special Sign is, mixing extraction is carried out to Chinese sumac leaf using different auxiliary extracting solutions in the step (2), the assisted extraction liquid is Ethanol, Extracting temperature are 50~70 DEG C, and the hot dipping for carrying out 1~3h carries 1~2 time, are filtered under diminished pressure to remove in extracting solution after constant volume and mix Particulate matter is closed, extracting solution centrifugal filtration is degerming to obtain Chinese sumac leaf crude extract.
- 6. a kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract according to claim 2, it is special Sign is, mixing extraction is carried out to Chinese sumac leaf using different auxiliary extracting solutions in the step (2), the assisted extraction liquid is Ethanol, Extracting temperature are 40~70 DEG C, and the ultrasonic wave extraction of every 2~4h 2~4 times, is filtered under diminished pressure after constant volume and removes in extracting solution Hybrid particles material, extracting solution centrifugal filtration is degerming to obtain Chinese sumac leaf crude extract.
- 7. a kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract according to claim 2, it is special Sign is, when assisted extraction liquid is ethanol, the volumetric concentration of ethanol is 60%.
- 8. a kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract according to claim 1, it is special Sign is that chlorella condition of culture is：Using the BG11 culture mediums Jing Guo filtration sterilization in the triangle by autoclave sterilization It is inoculated with and cultivates in flask, the chlorella being inoculated with is placed in artificial climate incubator in culture, and temperature is 23 ± 1 DEG C, illumination Intensity is 2000~6000lx, Light To Dark Ratio 12h：12h, inoculation in every 12~16 days once, chlorella are uniformly distributed to obtain Enough illumination.
- 9. a kind of method for promoting rapid growth of chlorella using Chinese sumac leaf extract according to claim 1, it is special Sign is that the growth promotion rate of chlorella at least doubles after the 3rd day.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108676768A (en) * | 2018-07-27 | 2018-10-19 | 中国科学院合肥物质科学研究院 | A kind of chlorella growth accelerating agent and preparation method thereof |
| CN108892563A (en) * | 2018-07-26 | 2018-11-27 | 中国科学院合肥物质科学研究院 | A kind of production selenium promotor and preparation method thereof for improving chlorella and producing organic selenium |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102864117A (en) * | 2012-09-25 | 2013-01-09 | 北京林业大学 | Method for promoting rapid growth of chlorella by using Chinese parasol fallen leaves |
| FR2985183A1 (en) * | 2011-12-30 | 2013-07-05 | Dermance | Topical composition, useful for treating skin aging, comprises a combination of enzymatic exfoliant action composition and active agent present in a flushable mask applied on skin |
| CN103768109A (en) * | 2012-10-25 | 2014-05-07 | 奇复康药物研发(苏州)有限公司 | Extraction method for Chinese parasol tree bark flavonoid components |
| CN106381269A (en) * | 2016-08-30 | 2017-02-08 | 浙江工商大学 | Method for increasing yield of chlorella by ultrasonic extraction of rice leaf |
-
2017
- 2017-12-29 CN CN201711478930.8A patent/CN108004195B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2985183A1 (en) * | 2011-12-30 | 2013-07-05 | Dermance | Topical composition, useful for treating skin aging, comprises a combination of enzymatic exfoliant action composition and active agent present in a flushable mask applied on skin |
| CN102864117A (en) * | 2012-09-25 | 2013-01-09 | 北京林业大学 | Method for promoting rapid growth of chlorella by using Chinese parasol fallen leaves |
| CN103768109A (en) * | 2012-10-25 | 2014-05-07 | 奇复康药物研发(苏州)有限公司 | Extraction method for Chinese parasol tree bark flavonoid components |
| CN106381269A (en) * | 2016-08-30 | 2017-02-08 | 浙江工商大学 | Method for increasing yield of chlorella by ultrasonic extraction of rice leaf |
Non-Patent Citations (1)
| Title |
|---|
| 陈存武 等: "盐肤木总黄酮的提取研究", 《湖南农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108892563A (en) * | 2018-07-26 | 2018-11-27 | 中国科学院合肥物质科学研究院 | A kind of production selenium promotor and preparation method thereof for improving chlorella and producing organic selenium |
| CN108676768A (en) * | 2018-07-27 | 2018-10-19 | 中国科学院合肥物质科学研究院 | A kind of chlorella growth accelerating agent and preparation method thereof |
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