CN104928152A - Photo-bioreactor - Google Patents
Photo-bioreactor Download PDFInfo
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- CN104928152A CN104928152A CN201510357644.0A CN201510357644A CN104928152A CN 104928152 A CN104928152 A CN 104928152A CN 201510357644 A CN201510357644 A CN 201510357644A CN 104928152 A CN104928152 A CN 104928152A
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- bioreactor
- pipe
- aeration tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
Abstract
The invention discloses a photo-bioreactor, and relates to the technical field of photo-biological cultivation. By the photo-bioreactor, photo-biological cultivation yield can be improved. The photo-bioreactor comprises a hollow reactor body and an aerating device positioned in the reactor body. The reactor body comprises at least one light reception face, and the aerating device is used for blowing air in the direction towards the light reception faces. The photo-bioreactor is used for photo-biological cultivation.
Description
Technical field
The present invention relates to photo bio cultural technique field, particularly relate to a kind of bioreactor.
Background technology
Photo bio is that one can carry out photosynthetic photoautotroph, and light energy conversion is chemical energy by photosynthesis by photo bio, makes photo bio have widespread use in all many-sides such as bioenergy, biological sequestration, environmental protection, food, feed and medicine.At present, the main bioreactor that adopts cultivates in a large number to photo bio, in breeding process, photo bio is mixed in the culturing liquid in bioreactor and cultivates.
But, present inventor finds, owing to being subject to the impact of the density of photo bio in culturing liquid, decay rapidly during light penetration culturing liquid, the distance that light can penetrate is only several millimeters, make on vertical sensitive surface direction, be only light district near the region of sensitive surface several millimeters, outside be all dark space, the part photo bio in bioreactor can be caused like this to be in light district or dark space for a long time, and the photosynthesis of photo bio comprises photoresponse and two stages of dark reaction, photoresponse is carried out in photo bio circulation and dark reaction can complete photosynthesis, photo bio cannot circulate and carry out photoresponse and dark reaction, be unfavorable for that photo bio carries out photosynthesis, be unfavorable for the cultured output improving photo bio.
Summary of the invention
The object of the present invention is to provide a kind of bioreactor, for improving the cultured output of photo bio.
For achieving the above object, the invention provides a kind of bioreactor, adopting following technical scheme:
A kind of bioreactor, comprise the reactor body of hollow, described reactor body has at least one sensitive surface, and described bioreactor also comprises the aerating apparatus being positioned at described reactor body, and described aerating apparatus is used for jet towards the direction of sensitive surface.
Aerating apparatus is comprised in bioreactor provided by the invention, this aerating apparatus is used for jet towards the direction of sensitive surface, thus culturing liquid can be taken exercises along the jet direction of aerating apparatus, culturing liquid is circulated between dark space and light district, and then the photo bio in drive culturing liquid shifts between dark space and light district, namely by the jet photo bio that regulates at the cycle frequency of light district and dark space, control photo bio in light district and the dark space residence time, make the photo bio in culturing liquid can not be in light district or dark space for a long time, and then photo bio can be circulated carry out photoresponse and dark reaction, be conducive to photo bio and carry out photosynthesis, be conducive to the cultured output improving photo bio.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, below the accompanying drawing used required in describing embodiment is briefly described, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
The schematic diagram of the first bioreactor that Fig. 1 provides for the embodiment of the present invention;
The schematic diagram of the air feeder that Fig. 2 provides for the embodiment of the present invention;
The schematic diagram of the aeration tube that Fig. 3 provides for the embodiment of the present invention;
The syndeton that Fig. 4 provides for the embodiment of the present invention and the annexation schematic diagram with aeration tube and air outlet thereof;
The schematic diagram of the second bioreactor that Fig. 5 provides for the embodiment of the present invention;
The schematic diagram of the third bioreactor that Fig. 6 provides for the embodiment of the present invention.
Description of reference numerals:
1-reactor body; 11-sensitive surface; 2-aerating apparatus;
21-air feeder; 211-inlet mouth; 212-air outlet;
22-aeration tube; 221-pipe; 222-end face;
223-the first ventilating pit; 224-ring structure; 225-the second ventilating pit;
23-syndeton.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment one
Embodiments provide a kind of bioreactor, as shown in Figure 1, this bioreactor comprises: the reactor body 1 of hollow, reactor body 1 has at least one sensitive surface 11, this bioreactor also comprises the aerating apparatus 2 being positioned at reactor body 1, and this aerating apparatus 2 is for jet towards the direction of sensitive surface 11.Wherein, the gas that aerating apparatus 2 sprays is preferably the mixed gas of air and carbonic acid gas, in order to avoid have a negative impact to the cultivation of photo bio.This bioreactor may be used for cultivating microalgae, such as Nannochloropsis oculata.
Aerating apparatus is comprised in the bioreactor that the embodiment of the present invention provides, this aerating apparatus is used for jet towards the direction of sensitive surface, thus culturing liquid can be taken exercises along the jet direction of aerating apparatus, culturing liquid is circulated between dark space and light district, and then the photo bio in drive culturing liquid shifts between dark space and light district, namely by the jet photo bio that regulates at the cycle frequency of light district and dark space, control photo bio in light district and the dark space residence time, make the photo bio in culturing liquid can not be in light district or dark space for a long time, and then photo bio can be circulated carry out photoresponse and dark reaction, be conducive to photo bio and carry out photosynthesis, be conducive to the cultured output improving photo bio.
The concrete structure of above-mentioned aerating apparatus 2 can have multiple, as long as it can be used for jet towards the direction of sensitive surface 11.Exemplarily, as shown in Figure 1, aerating apparatus 2 comprises air feeder 21 and multiple aeration tube 22 of hollow, wherein, as shown in Figure 2, this air feeder 21 and multiple aeration tube 22 multiple air outlet 212 one to one, each aeration tube 22 is all connected on an air outlet 212.This air feeder 21 can also have at least one inlet mouth 211, and exemplarily, this air inlet port 211 can be connected with air compressor.In photo bio breeding process, the process of gas flow is as follows: the mixed gas of carbonic acid gas and air passes into air feeder 21 by the inlet mouth 211 on air feeder 21, then aeration tube 22 is entered from the air outlet 212 of air feeder 21, finally spray from aeration tube 22, to make aerating apparatus 2 to jet towards the direction of sensitive surface 11.
In addition, due in the breeding process of photo bio, when the intensity of the light that external light source irradiates changes, the distance of the culturing liquid that light can penetrate also can change, and then the thickness of the light district of culturing liquid and dark space (Ji Guang district and dark space are along the degree of depth perpendicular to sensitive surface direction) is changed.And photo bio carries out the requirement that photoresponse has photon numbers how many, normal light biology carries out a photoresponse needs 8 photons.When intensity of illumination is large, the quantity that photo bio obtains photon in the unit time is many, so photo bio needs the time of stop short in light district; When intensity of illumination is weak, the quantity of the photon that photo bio obtains within the unit time is few, so photo bio needs the time of stop long in light district, to absorb enough number of photons carrying out photoresponse.If the situation of aerating apparatus 2 jet flow stream can not change along with intensity of illumination, then can not regulate, control the cycle frequency of photo bio in light district and dark space and the residence time, be unfavorable for that photoresponse and dark reaction are carried out in photo bio circulation, be unfavorable for the cultured output improving photo bio.
Therefore, in order to solve the problem, improve the cultured output of photo bio, following three kinds of structures of aeration tube 22 are provided in the embodiment of the present invention, wherein, when aeration tube 22 is the first structure, the jet amount of aeration tube 22 is adjustable, when aeration tube 22 is the second structure, aeration tube 22 is scalable, thus make the distance between aeration tube 22 and sensitive surface 11 adjustable, when aeration tube 22 is the third structure, the distance between aeration tube 22 and sensitive surface 11 and jet amount all adjustable.
The first structure, as shown in Figure 3, each aeration tube 22 is comprise the N level pipe 221 be set with successively from inside to outside, and the two-stage pipe 221 of arbitrary neighborhood can circumferentially relatively rotate in direction, N be more than or equal to 2 positive integer.Particularly, one end of the 1st grade of pipe 221 is opening, and the other end is end face 222, end face 222 is provided with multiple first ventilating pit 223; One end of i-th grade of pipe 221 is opening, the other end is end face 222, end face 222 has multiple first ventilating pits 223 and the circular open corresponding with the i-th-1 grade pipe, and the diameter of circular open on the end face 222 of i-th grade of pipe 221 is identical with the diameter of the i-th-1 grade pipe 221; And, the i-th-1 grade pipe 221 also comprises the outward extending ring structure 224 from one end of opening, the external diameter of ring structure 224 is identical with the diameter of i-th grade of pipe 221, ring structure 224 is provided with multiple second ventilating pit 225, second ventilating pit 225 and the first ventilating pit 223 one_to_one corresponding and measure-alike, i is the positive integer being more than or equal to 2 and being less than or equal to N.
There is the aeration tube 22 of the first structure in use, when between any two-stage pipe 221 of mutual sheathing, circumferentially direction relatively rotates, position relationship between the first ventilating pit 223 on the second ventilating pit 225 in the ring structure 224 that the i-th-1 grade pipe 221 comprises and the end face 222 of i-th grade of pipe 221 will change, the two can overlap completely, now the jet amount of the end face 222 of i-th grade of pipe 221 is maximum, the two can partially overlap, now the jet amount of the i-th-1 grade pipe 221 is placed in the middle, the two also can complete and not overlap, now the i-th-1 grade pipe 221 is not jet.Therefore, when the aeration tube 22 of bioreactor has the first structure, the jet amount of aeration tube 22 is adjustable, thus photo bio can be regulated at the length of the residence time in light district and the photo bio cycle frequency in light district and dark space, to adapt to the change of light intensity, carry out photoresponse and dark reaction to be conducive to photo bio circulation, and then improve the cultured output of photo bio.
The second structure, as shown in Figure 3, each aeration tube 22 is comprise the N level pipe 221 be set with successively from inside to outside, and the two-stage pipe 221 of arbitrary neighborhood can in axial direction relative movement, N be more than or equal to 2 positive integer.Particularly, one end of the 1st grade of pipe 221 is opening, and the other end is end face 222, end face 222 is provided with multiple first ventilating pit 223; One end of i-th grade of pipe 221 is opening, the other end is end face 222, end face have multiple first ventilating pits 223 and the circular open corresponding with the i-th-1 grade pipe, and the diameter of circular open on the end face 222 of i-th grade of pipe 221 is identical with the diameter of the i-th-1 grade pipe 221, i is the positive integer being more than or equal to 2 and being less than or equal to N.
There is the aeration tube 22 of the second structure in use, between any two-stage pipe 221 of mutual sheathing during in axial direction relative movement, distance between the end face 222 of the pipes at different levels 221 in aeration tube 22 and sensitive surface 11 can be regulated, realize compression and the stretching of aeration tube 22, and then photo bio can be regulated at the length of the residence time in light district and the photo bio cycle frequency in light district and dark space, to adapt to the change of light intensity, carry out photoresponse and dark reaction to be conducive to photo bio circulation, and then improve the cultured output of photo bio.
Exemplarily, when light intensity strengthens gradually, above-mentioned aeration tube 22 should be made to stretch, distance between the end face 222 of each pipe 221 and sensitive surface 11 reduces, to make photo bio shorter in the residence time in light district, photo bio is larger at the cycle frequency of light district and dark space, when light intensity weakens gradually, above-mentioned aeration tube 22 should be made to shrink, distance between the end face 222 of each pipe 221 and sensitive surface 11 increases, to make photo bio longer in the residence time in light district, photo bio is less at the cycle frequency of light district and dark space, to realize under the irradiate light of varying strength, photo bio all can circulate and carry out photoresponse and dark reaction, and then improve the cultured output of photo bio.
The third structure, as shown in Figure 3, on the basis of the second structure, the two-stage pipe 221 of arbitrary neighborhood also can circumferentially relatively rotate in direction; And, the i-th-1 grade pipe 221 also comprises the outward extending ring structure 224 from one end of opening, the external diameter of ring structure 224 is identical with the diameter of i-th grade of pipe 221, ring structure 224 is provided with multiple second ventilating pit 225, second ventilating pit 225 and the first ventilating pit 223 one_to_one corresponding and measure-alike, i is the positive integer being more than or equal to 2 and being less than or equal to N.
There is the aeration tube 22 of the third structure in use, distance between aeration tube 22 and sensitive surface 11 and jet amount all adjustable, thus photo bio can be regulated at the length of the residence time in light district and the photo bio cycle frequency in light district and dark space, to adapt to the change of light intensity, carry out photoresponse and dark reaction to be conducive to photo bio circulation, and then improve the cultured output of photo bio.Its concrete regulative mode is described in detail in the first structure and the second structure, no longer carries out the description repeated herein.
The maximum diameter of the N level pipe 221 mentioned in above-mentioned three kinds of structures is not more than 10 centimetres, be advisable, and the length of every grade of pipe 221 is advisable with 3 ~ 5 centimetres with 3 ~ 5 centimetres.In actual application, with the increase of bioreactor light path, the maximum diameter of pipe 221 at different levels also increases thereupon, and the progression of the pipe 221 that each aeration tube 22 comprises also increases thereupon, but should ensure aeration tube 22 after stretching to the light path being no more than bioreactor during maximum length.
In addition, present inventor finds, when aerating apparatus 2 is jet along the direction of the boundary perpendicular to light district and dark space, the flow effect of culturing liquid between light district and dark space is best, but when adopting natural light as external light source, angle between light and sensitive surface 11 also can change along with the change of time, thus the light district of culturing liquid and dark space are changed, cause aerating apparatus 2 cannot direction always along the boundary perpendicular to light district and dark space jet, and then make culturing liquid between light district and dark space to circulate effect undesirable.In order to solve the problem, as shown in Figure 4, the bioreactor provided in the embodiment of the present invention can also comprise the syndeton 23 of hollow, each aeration tube 22 is all connected on an air outlet 212 by a syndeton 23, wherein, angle between the axis of one end that syndeton 23 is connected with aeration tube 22 and the normal direction of sensitive surface 11 is adjustable, the jet direction of the aeration tube 22 connected to make it is adjustable, and then make aerating apparatus 2 can direction always along the boundary perpendicular to light district and dark space jet, with make culturing liquid between light district and dark space to circulate effect better.
Realize for the ease of those skilled in the art, the invention provides a kind of concrete structure of syndeton 23, exemplarily, as shown in Figure 4, syndeton 23 is the tubular structure that surface has fold, one end of tubular structure is connected on air outlet 212, and the other end of tubular structure is connected to bottom aeration tube 22.On tubular structure, the fold of side is stretched, and when the fold of its opposite side is compressed, thus tubular structure is bent, thus can regulate the angle between the axis of one end that tubular structure is connected with aeration tube 22 and the normal direction of sensitive surface 11.When aeration tube 22 comprises N level pipe, the bottom of aeration tube 22 refers to one end towards air feeder 21 of N level pipe.Exemplarily, the two ends of tubular structure can be connected respectively with bottom air outlet 212 and aeration tube 22 by screw, now, screw all should be provided with bottom the two ends of tubular structure and air outlet 212 and aeration tube 22, the two ends of certain tubular structure also can be connected by other modes such as bondings respectively with bottom air outlet 212 and aeration tube 22, and the embodiment of the present invention does not limit this.
It should be noted that, the concrete structure of syndeton 23 is not limited to this, and those skilled in the art can select according to actual needs.Such as, syndeton 23 can select Authorization Notice No. for the structure described in the patent of CN201696738U, and because this structure has been described in detail in that patent, the embodiment of the present invention no longer repeats.
In addition, according to the needs of photo bio cultivation in reality, the bioreactor that the embodiment of the present invention provides can be box like structure or tubular structure.Exemplarily, as shown in Figure 1, when bioreactor is box like structure, and this bioreactor is when having two relative sensitive surfaces 11, air feeder 21 is tabular, and air feeder 21 is positioned at the centre of bioreactor, and two sides of air feeder 21 are respectively towards two sensitive surfaces 11, two sides of air feeder 21 all have multiple air outlets (not shown), each air outlet all connects an aeration tube 22.As shown in Figure 5, when bioreactor is box like structure, and this bioreactor is when having a sensitive surface 11, air feeder 21 is tabular, air feeder 21 is arranged in the bioreactor side relative with sensitive surface 11, air feeder 21 on the side of sensitive surface 11, there is multiple air outlets (not shown), each air outlet all connects an aeration tube 22.As shown in Figure 6, when bioreactor is tubular structure, and the side of tubular structure is when being sensitive surface 11, air feeder 21 is rectangular structure or cylindrical structure, air feeder 21 is positioned on the axis of bioreactor, each side of air feeder 21 all has multiple air outlets (not shown), each air outlet all connects an aeration tube 22.
Embodiment two
The advantage of the bioreactor in the present invention is understood for the ease of those skilled in the art, in the embodiment of the present invention, the bioreactor adopted in the present invention is carried out to the cultured output of Nannochloropsis oculata cultivation, and the cultured output adopting bioreactor of the prior art to carry out Nannochloropsis oculata cultivation contrasts.
Contrast experiment one
Bioreactor as shown in Figure 1 provided by the invention is adopted to carry out Nannochloropsis oculata cultivation, this bioreactor is box like structure, and this bioreactor has two relative sensitive surfaces 11, air feeder 21 is tabular, air feeder 21 is positioned at the centre of bioreactor, two sides of air feeder 21, respectively towards two sensitive surfaces 11, two sides of air feeder 21 all have multiple air outlet 212, each air outlet 212 all connect an aeration tube 22.The specification of this bioreactor is meter (height) × 0.2,1 meter of (length) × 1 meter (wide), and the specification of air feeder 21 is meter (height) × 0.02,1 meter of (length) × 0.8 meter (wide).Adopt natural light as external light source, two sensitive surfaces 11 of this bioreactor are respectively towards east-west direction, use this bioreactor to cultivate the algae liquid that initial inoculation concentration is 1 grams per liter, and utilize air compressor in air feeder 21, pass into the mixed gas of carbon dioxide and oxygen.
Breeding process is specific as follows: first, the morning about 8, aeration tube 22 towards east is stretched, aeration tube 22 is made to be elongated to sensitive surface 1 ~ 3 centimeters of its first step pipe distance towards east, rotate pipe 221 at different levels again, be adjusted to 1/4 with the second ventilating pit 225 in the ring structure 224 comprised by pipe 221 at different levels with the overlap ratio of the first ventilating pit 223 on the end face 222 of next stage pipe 221; Secondly, at eight between ten one, every one hour, the angle between the axis of each aeration tube 22 towards east and the normal direction of sensitive surface 11 is upwards increased 15 degree, the overlap ratio of the first ventilating pit 223 and the second ventilating pit 225 is increased by 1/4 simultaneously; Again, from afternoon about ten four, aeration tube 22 towards east is shunk, aeration tube 22 is made to shorten to sensitive surface 5 ~ 7 centimeters of its first step pipe distance towards east, the aeration tube 22 be exposed to the west is stretched simultaneously, sensitive surface 1 ~ 3 centimeters making aeration tube 22 be elongated to its first step pipe distance to be exposed to the west, then the angle between the axis of each aeration tube 22 and the normal direction of sensitive surface 11 is upwards adjusted to 45 degree, make the first ventilating pit 223 and the second ventilating pit 225 overlap completely simultaneously; Finally, between ten four o'clock to ten seven, every one hour, the angle between the axis of each aeration tube 22 and the normal direction of sensitive surface 11 is reduced 15 degree downwards, the overlap ratio of the first ventilating pit 223 and the second ventilating pit 225 is reduced 1/4 simultaneously.
When adopting the bioreactor of prior art to carry out Nannochloropsis oculata cultivation, be only in bioreactor without aerating apparatus with the difference of above-mentioned breeding process.
Adopt continuous five days of bioreactor cultivation Nannochloropsis oculata as shown in Figure 1 provided by the invention, the yield per unit of Nannochloropsis oculata reaches 25.6 grams/m/day, and phototranstormation efficiency is 4.8%.And adopt the bioreactor of prior art to cultivate continuous five days of Nannochloropsis oculata.The yield per unit of Nannochloropsis oculata is 15.8 grams/m/day, and phototranstormation efficiency is 3.8%.As can be seen from above data, adopt bioreactor provided by the invention to cultivate Nannochloropsis oculata, substantially increase the cultured output of Nannochloropsis oculata.
Contrast experiment two
Bioreactor as shown in Figure 5 provided by the invention is adopted to carry out Nannochloropsis oculata cultivation, this bioreactor is box like structure, and this bioreactor has a sensitive surface 11, air feeder 21 is tabular, air feeder 21 is arranged in the bioreactor side relative with sensitive surface 11, air feeder 21 on the side of sensitive surface 11, there is multiple air outlet 212, each air outlet 212 all connects an aeration tube 22.The specification of this bioreactor is meter (height) × 0.1,1 meter of (length) × 1 meter (wide), and the specification of air feeder 21 is meter (height) × 0.02,1 meter of (length) × 0.8 meter (wide).Adopt natural light as external light source, the sensitive surface 11 of this bioreactor is towards south, use this bioreactor to cultivate the algae liquid that initial inoculation concentration is 1 grams per liter, and utilize air compressor in air feeder 21, pass into the mixed gas of carbon dioxide and oxygen.
Breeding process is specific as follows: first, the morning about 8, aeration tube 22 is stretched, aeration tube 22 is made to be elongated to its first step pipe distance sensitive surface 1 ~ 3 centimeters, rotate pipe 221 at different levels again, be adjusted to 1/4 with the second ventilating pit 225 in the ring structure 224 comprised by pipe 221 at different levels with the first overlapping of ventilating pit 223 on the end face 222 of next stage pipe 221, and each aeration tube 22 regulating eastwards, is 60 degree to make the angle between the normal direction of its axis and sensitive surface 11; Secondly, at eight between ten one, every one hour, each aeration tube 22 axially regulated 15 degree westwards, simultaneously by the overlap ratio increase by 1/4 of the first ventilating pit 223 and the second ventilating pit 225; Again, from afternoon about ten four, by the axially adjustment 15 degree westwards of each aeration tube 22, aeration tube 22 is shunk, make aeration tube 22 shorten to its first step pipe distance sensitive surface 3 ~ 5 centimeters; Finally, between ten four o'clock to ten seven, every one hour, by the axially adjustment 15 degree westwards of aeration tube 22, the overlap ratio of the first ventilating pit 223 and the second ventilating pit 225 is reduced 1/4 simultaneously.
When adopting the bioreactor of prior art to carry out Nannochloropsis oculata cultivation, be only in bioreactor without aerating apparatus with the difference of above-mentioned breeding process.
Adopt continuous five days of bioreactor cultivation Nannochloropsis oculata as shown in Figure 4 provided by the invention, the yield per unit of Nannochloropsis oculata reaches 28.8 grams/m/day, and phototranstormation efficiency is 5.4%.And adopt the bioreactor of prior art to cultivate continuous five days of Nannochloropsis oculata.The yield per unit of Nannochloropsis oculata is 16.3 grams/m/day, and phototranstormation efficiency is 4.0%.As can be seen from above data, adopt bioreactor provided by the invention to cultivate Nannochloropsis oculata, substantially increase the cultured output of Nannochloropsis oculata.
From above two contrast experiments, adopt bioreactor provided by the invention greatly can improve the cultured output of photo bio.
The above; be only the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, is anyly familiar with those skilled in the art in the technical scope that the present invention discloses; change can be expected easily or replace, all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain of described claim.
Claims (10)
1. a bioreactor, comprise the reactor body of hollow, described reactor body has at least one sensitive surface, it is characterized in that, described bioreactor also comprises the aerating apparatus being positioned at described reactor body, and described aerating apparatus is used for jet towards the direction of described sensitive surface.
2. bioreactor according to claim 1, is characterized in that, described aerating apparatus comprises the air feeder of hollow and multiple aeration tube, and described air feeder comprises the multiple air outlets connected one to one with multiple aeration tube.
3. bioreactor according to claim 2, is characterized in that, described aeration tube is comprise the N level pipe be set with successively from inside to outside, N be more than or equal to 2 positive integer, the two-stage pipe of arbitrary neighborhood can in axial direction relative movement;
Wherein, one end of the 1st grade of pipe is opening, and the other end is end face, described end face is provided with multiple first ventilating pit; One end of i-th grade of pipe is opening, the other end is end face, described end face have multiple first ventilating pit and the circular open corresponding with the i-th-1 grade pipe, and the diameter of circular open on the end face of i-th grade of pipe is identical with the diameter of the i-th-1 grade pipe, i is the positive integer being more than or equal to 2 and being less than or equal to N.
4. bioreactor according to claim 3, is characterized in that, the two-stage pipe of arbitrary neighborhood can circumferentially relatively rotate in direction; And, the i-th-1 grade pipe also comprises from the outward extending ring structure in one end of opening, the external diameter of described ring structure is identical with the diameter of i-th grade of pipe, described ring structure is provided with multiple second ventilating pit, described second ventilating pit and described first ventilating pit one_to_one corresponding and measure-alike, i is the positive integer being more than or equal to 2 and being less than or equal to N.
5. bioreactor according to claim 2, is characterized in that, described aeration tube is comprise the N level pipe be set with successively from inside to outside, N be more than or equal to 2 positive integer, the two-stage pipe of arbitrary neighborhood can circumferentially relatively rotate in direction;
Wherein, one end of the 1st grade of pipe is opening, and the other end is end face, described end face is provided with multiple first ventilating pit; One end of i-th grade of pipe is opening, and the other end is end face, described end face has multiple first ventilating pit and the circular open corresponding with the i-th-1 grade pipe, and the diameter of circular open on the end face of i-th grade of pipe is identical with the diameter of the i-th-1 grade pipe;
And, the i-th-1 grade pipe also comprises from the outward extending ring structure in one end of opening, the external diameter of described ring structure is identical with the diameter of i-th grade of pipe, described ring structure is provided with multiple second ventilating pit, described second ventilating pit and described first ventilating pit one_to_one corresponding and measure-alike, i is the positive integer being more than or equal to 2 and being less than or equal to N.
6. bioreactor according to claim 2, it is characterized in that, also comprise the syndeton of hollow, each described aeration tube is all connected on a described air outlet by a described syndeton, and the angle between the axis of one end that described syndeton is connected with described aeration tube and the normal direction of described sensitive surface is adjustable.
7. bioreactor according to claim 6, it is characterized in that, described syndeton is the tubular structure that surface has fold, and one end of described tubular structure is connected on described air outlet, and the other end of described tubular structure is connected to bottom described aeration tube.
8. the bioreactor according to any one of claim 2-7, it is characterized in that, described bioreactor is box like structure, described bioreactor has two relative sensitive surfaces, described air feeder is tabular, described air feeder is positioned at the centre of described bioreactor, two sides of described air feeder are respectively towards two described sensitive surfaces, two sides of described air feeder all have multiple described air outlet, each described air outlet all connects a described aeration tube.
9. the bioreactor according to any one of claim 2-7, it is characterized in that, described bioreactor is box like structure, described bioreactor has a sensitive surface, described air feeder is tabular, described air feeder is arranged in the described bioreactor side relative with described sensitive surface, described air feeder on the side of described sensitive surface, there is multiple described air outlet, each described air outlet all connects a described aeration tube.
10. the bioreactor according to any one of claim 2-7, it is characterized in that, described bioreactor is tubular structure, the side of described tubular structure is sensitive surface, described air feeder is rectangular structure or cylindrical structure, described air feeder is positioned on the axis of described bioreactor, and each side of described air feeder all has multiple described air outlet, each described air outlet all connects a described aeration tube.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510357644.0A CN104928152B (en) | 2015-06-24 | 2015-06-24 | A kind of bioreactor |
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| CN201510357644.0A CN104928152B (en) | 2015-06-24 | 2015-06-24 | A kind of bioreactor |
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| CN104928152A true CN104928152A (en) | 2015-09-23 |
| CN104928152B CN104928152B (en) | 2017-06-09 |
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| US20090047722A1 (en) * | 2005-12-09 | 2009-02-19 | Bionavitas, Inc. | Systems, devices, and methods for biomass production |
| JP2012191894A (en) * | 2011-03-17 | 2012-10-11 | Ihi Corp | Culture apparatus |
| CN103525686A (en) * | 2013-10-24 | 2014-01-22 | 重庆大学 | Composite reinforced microalgae photobioreactor based on hollow light pipes |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US20090047722A1 (en) * | 2005-12-09 | 2009-02-19 | Bionavitas, Inc. | Systems, devices, and methods for biomass production |
| JP2012191894A (en) * | 2011-03-17 | 2012-10-11 | Ihi Corp | Culture apparatus |
| CN103525686A (en) * | 2013-10-24 | 2014-01-22 | 重庆大学 | Composite reinforced microalgae photobioreactor based on hollow light pipes |
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