CN110218653A - A kind of chlorella animal feed production technology - Google Patents
A kind of chlorella animal feed production technology Download PDFInfo
- Publication number
- CN110218653A CN110218653A CN201910516916.5A CN201910516916A CN110218653A CN 110218653 A CN110218653 A CN 110218653A CN 201910516916 A CN201910516916 A CN 201910516916A CN 110218653 A CN110218653 A CN 110218653A
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- chlorella
- production technology
- animal feed
- density
- flask
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Abstract
The invention discloses a kind of chlorella animal feed production technology, the step of production technology, is as follows: the appliance device in workshop is carried out disinfection processing；Culture medium is made, and chlorella mother cell is placed on it and is cultivated；Chlorella cells on culture medium in second step are screened；Chlorella cells after screening in third step are put into test tube, carry out test tube cultivation；Chlorella after cultivating in four steps is seeded to and continues growing operation in the flask that capacity is 100 milliliters and is subject to light-illuminating.The present invention is settled so that alga cells are able to carry out a large amount of breeding by flocculation concentration, then drying is concentrated into small size, finally carries out centrifugally operated again, realizes the quick obtaining to chlorella in reactor tank, the chlorella of acquisition is ground into powdery again, is added in animal feed；By above-mentioned production technology, the production efficiency of chlorella is improved, and ensure that the output quality of chlorella.
Description
Technical field
The present invention relates to chlorellas to produce and process technical field, specially a kind of chlorella animal feed production technology.
Background technique
Chlorophyta, eucaryote are a kind of spherical unicellular algas, are one of life earliest on the earth, are a kind of high
The light of effect and plant lean on photoautotrophy growth and breeding.Chlorella is limnetic chlorella, it by sunlight, water and carbon dioxide, with
The vigorous fertility of 4 cells was divided out every 24-28 hours, and ceaselessly solar energy is converted into and contains a variety of nutrition
The frond of ingredient, and a large amount of oxygen are discharged in increment；Its vegetable protein content accounts for 50% or more, fat content 6-7%,
Its amino acid composition is also extremely excellent.Containing several mineral materials and micro-element, cellulosic, folic acid, nucleic acid, calcium, magnesium, iron, zinc and
The nutrition of the equilibrium such as several mineral materials vitamin；Algae powder is added in animal feed, and the speed of growth of animal can be improved, and improves and raises
Expect conversion ratio, and reduces disease；Algae powder is added in milk cow and egg feedstuff, its milk, the iodine in egg and vitamin A can be improved
Content.Algae powder is added in aquatic animal feed, can improve weight gain, enhances premunition, and there is good effect；However in reality
In production process, the production and processing technology Shortcomings of chlorella cause production efficiency not efficient enough, and quality also it is irregular not
Together.
Summary of the invention
The purpose of the present invention is to provide a kind of chlorella animal feed production technologies, improve to conventional apparatus, solution
It has determined the problems in background technique.
To achieve the above object, the invention provides the following technical scheme: a kind of chlorella animal feed production technology, special
The step of sign is the production technology is as follows:
S1: the appliance device in workshop is carried out disinfection processing；
S2: production culture medium, and chlorella mother cell is placed on it and is cultivated；
S3: the chlorella cells on culture medium in S2 are screened；
S4: the chlorella cells after screening in S3 are put into test tube, carry out test tube cultivation；
S5: the chlorella after cultivating in S4 step is seeded to and continues to cultivate behaviour in the flask that capacity is 100 milliliters
Make and is subject to light-illuminating；
S6: after the density of chlorella reaches standard density in flask in S5 step, by the bead after being cultivated in S5 step
Algae, which is seeded to, to be continued growing operation in the flask that capacity is 1,000 milliliters and is subject to light-illuminating；
S7: after the density of chlorella reaches standard density in flask in S6 step, by the bead after being cultivated in S6 step
Algae, which is seeded to, to be continued growing operation in the flask that capacity is 5,000 milliliters and is subject to light-illuminating；
S8: after the density of chlorella reaches standard density in flask in S7 step, by the bead after being cultivated in S7 step
It is in eight hectoliters of reactor tank to continue growing operation and supplemental oxygen, carbon dioxide and be subject to light that algae, which is seeded to volume,
Irradiation；
S9: small after being cultivated in S8 step after the density of chlorella reaches standard density in reactor tank in S8 step
Ball algae, which is divided into 20 parts and is seeded in the reactor tank that 20 independent volumes are eight hectoliters, continues growing operation
And supplemental oxygen, carbon dioxide and it is subject to light-illuminating；
S10: the bead algae solution in S9 step in reactor tank is taken to carry out Density Detection；
S11: the reactor tank of the compound standard of density takes out the chlorella in it；
S12: the chlorella powder obtained in S11 is broken into powdery, is added in animal feed.
Preferably, the appliance device in the workshop includes that flask, reactor tank, laboratory and workshop sterilizing are set
It is standby.
Preferably, the workshop sterilizer uses ultraviolet radiation for sterilizing and disinfecting.
Preferably, the S5 is inoculated with into S9 operates filter screen precision that water used uses as 0. 002 millimeters.
Preferably, chlorella of the preferred diameter range in three to five micron diameter sizes in screening process in the S3.
Preferably, the chlorella in the S11 in reactor tank first passes through flocculation concentration sedimentation, and then drying is concentrated into corpusculum
Product, is finally centrifuged acquisition again.
Preferably, the workshop sterilizer uses ozone sterilization.
Compared with prior art, beneficial effects of the present invention are as follows:
One, the present invention so that alga cells are able to carry out a large amount of breeding, and finally makes by S1 to the progress of S9 step
The density of chlorella reaches standard density in reactor tank；
Two, the present invention is settled by flocculation concentration, and then drying is concentrated into small size, finally carries out centrifugally operated again, real
Now to the quick obtaining of chlorella in reactor tank, the chlorella of acquisition is ground into powdery again, is added in animal feed；
Three, the present invention improves the production efficiency of chlorella, and ensure that by the step in above-mentioned production technology
The output quality of chlorella.
Detailed description of the invention
Fig. 1 is process flow chart of the invention.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Referring to Fig. 1, the present invention provides a kind of technical solution: a kind of chlorella animal feed production technology, feature exist
It is as follows in the production technology the step of:
S1: the appliance device in workshop is carried out disinfection processing, and the appliance device in workshop includes flask, anti-
The culture equipment for breeding of tank, laboratory and workshop sterilizer, flask and reactor tank as chlorella is answered, in laboratory
The density of chlorella is measured, workshop sterilizer uses ultraviolet radiation for sterilizing and disinfecting, and ultraviolet radiation for sterilizing and disinfecting is benefit
The DNA (DNA) or RNA (ribonucleic acid) in microflora organisms cell can be destroyed with the ultraviolet light of appropriate wavelength
Molecular structure, cause growth cell death and (or) regenerative cell dead, achieve the effect that sterilizing.
S2: chlorella mother cell and is placed on it and cultivates by production culture medium, culture medium, refer to supply microorganism,
Plant or animal (or tissue) growth and breeding, the nutrient matrix as made of different nutriments formulated in combination.Generally all contain
Several big substances such as carbohydrate, nitrogen substance, inorganic salts (including microelement), vitamin and water.Culture medium is both to mention
For the living environment of cytotrophy and the basic substance and the cell growth and breeding that promote cell Proliferation, on culture medium, institute
The alga cells needed carry out preliminary breeding；
S3: screening the chlorella cells on culture medium in S2, in S3 in screening process preferred diameter range three
To the chlorella of five micron diameter sizes；
S4: the chlorella cells after screening in S3 are put into test tube, carry out test tube cultivation, in test tube addition growth
Required carbohydrate, various minerals salts, inorganic matter and water increase by test tube cultivation so that chlorella further breeds
Quantity.
S5: the chlorella after cultivating in S4 step is seeded to and continues to cultivate behaviour in the flask that capacity is 100 milliliters
Make and be subject to light-illuminating, in flask, the breeding of alga cells further progress cooperates illumination, raw using algae photoautotrophy
It is long, the supply of nutrition when further increasing breeding；
S6: after the density of chlorella reaches standard density in flask in S5 step, by the bead after being cultivated in S5 step
Algae, which is seeded to, to be continued growing operation in the flask that capacity is 1,000 milliliters and is subject to light-illuminating；Above-mentioned standard density is two
Million every milliliter, when inoculation the temperature of required water be maintained between 20 degrees Celsius to 28 degrees Celsius, by by water temperature
Control is between 20 degrees Celsius to 28 degrees Celsius, so that alga cells are able to maintain good activity, so that breeding is able to
Efficiently carry out；
S7: after the density of chlorella reaches standard density in flask in S6 step, by the bead after being cultivated in S6 step
It is to continue growing operation in 5,000 milliliters of flask and be subject to light-illuminating that algae, which is seeded to capacity, when cultivation required light
Light source is electrodeless lamp source, and illumination range is current collection also known as " electromagnetic induction lamp " between 5,000 luxs in 2,000 luxs
The technologies such as son, electromagnetism, vacuum in one the energy saving and environment friendly new light sources of international forth generation, have structure it is simple, it is electrodeless, without lamp
The features such as silk, high photosynthetic efficiency, high-color rendering and long-life, so that algae is able to carry out photosynthesis, is realized by the irradiation of light
Autotrophy provides enough nutrients for its breeding；
S8: after the density of chlorella reaches standard density in flask in S7 step, by the bead after being cultivated in S7 step
It is in eight hectoliters of reactor tank to continue growing operation and supplemental oxygen, carbon dioxide and be subject to light that algae, which is seeded to volume,
Irradiation；Guarantee that its photosynthetic is normally carried out by increasing carbon dioxide content；
S9: small after being cultivated in S8 step after the density of chlorella reaches standard density in reactor tank in S8 step
Ball algae, which is divided into 20 parts and is seeded in the reactor tank that 20 independent volumes are eight hectoliters, continues growing operation
And supplemental oxygen, carbon dioxide and it is subject to light-illuminating, S5 is inoculated with into S9 operates filter screen precision that water used uses as zero
002 millimeter of point, by carrying out high-precision filtering with water to inoculation, so that impurities in water particle greatly reduces.
S10: taking bead algae solution in S9 step in reactor tank to carry out Density Detection, and alga cells Board Lot is big when detection
It is as up to standard in 2,000,000 every milliliter；
S11: the reactor tank of the compound standard of density takes out the chlorella in it, and the chlorella in S11 in reactor tank is first led to
Flocculation concentration sedimentation is crossed, then drying is concentrated into small size, is finally centrifuged acquisition again, settles by flocculation concentration, then dries
It is concentrated into small size, finally carries out centrifugally operated again, realizes the quick obtaining to chlorella in reactor tank；
S12: the chlorella powder obtained in S11 is broken into powdery, is added in animal feed.
Referring to Fig. 1, the present invention provides another technical solution: a kind of chlorella animal feed production technology, feature
The step of being the production technology is as follows:
S1: the appliance device in workshop is carried out disinfection processing, and the appliance device in workshop includes flask, anti-
The culture equipment for breeding of tank, laboratory and workshop sterilizer, flask and reactor tank as chlorella is answered, in laboratory
The density of chlorella is measured, workshop sterilizer uses ozone sterilization, and the half-life period of ozone is 20 minutes or so,
Ozone after oxidation sterilizing sterilizing, extra oxygen atom 30~after forty minutes, and be combined into oxygen molecule, do not generate any poison and stay
Residual object, there is no the secondary pollution problems after sterilizing；Meanwhile increase room air oxygen content, also have it is very strong except it is mould,
The function of the organic matters peculiar smell such as awake, smelly.
S2: chlorella mother cell and is placed on it and cultivates by production culture medium, culture medium, refer to supply microorganism,
Plant or animal (or tissue) growth and breeding, the nutrient matrix as made of different nutriments formulated in combination.Generally all contain
Several big substances such as carbohydrate, nitrogen substance, inorganic salts (including microelement), vitamin and water.Culture medium is both to mention
For the living environment of cytotrophy and the basic substance and the cell growth and breeding that promote cell Proliferation, on culture medium, institute
The alga cells needed carry out preliminary breeding；
S3: screening the chlorella cells on culture medium in S2, in S3 in screening process preferred diameter range three
To the chlorella of five micron diameter sizes；
S4: the chlorella cells after screening in S3 are put into test tube, carry out test tube cultivation, in test tube addition growth
Required carbohydrate, various minerals salts, inorganic matter and water increase by test tube cultivation so that chlorella further breeds
Quantity.
S5: the chlorella after cultivating in S4 step is seeded to and continues to cultivate behaviour in the flask that capacity is 100 milliliters
Make and be subject to light-illuminating, in flask, the breeding of alga cells further progress cooperates illumination, raw using algae photoautotrophy
It is long, the supply of nutrition when further increasing breeding；
S6: after the density of chlorella reaches standard density in flask in S5 step, by the bead after being cultivated in S5 step
Algae, which is seeded to, to be continued growing operation in the flask that capacity is 1,000 milliliters and is subject to light-illuminating；Above-mentioned standard density is two
Million every milliliter, when inoculation the temperature of required water be maintained between 20 degrees Celsius to 28 degrees Celsius, by by water temperature
Control is between 20 degrees Celsius to 28 degrees Celsius, so that alga cells are able to maintain good activity, so that breeding is able to
Efficiently carry out；
S7: after the density of chlorella reaches standard density in flask in S6 step, by the bead after being cultivated in S6 step
It is to continue growing operation in 5,000 milliliters of flask and be subject to light-illuminating that algae, which is seeded to capacity, when cultivation required light
Light source is electrodeless lamp source, and illumination range is current collection also known as " electromagnetic induction lamp " between 5,000 luxs in 2,000 luxs
The technologies such as son, electromagnetism, vacuum in one the energy saving and environment friendly new light sources of international forth generation, have structure it is simple, it is electrodeless, without lamp
The features such as silk, high photosynthetic efficiency, high-color rendering and long-life, so that algae is able to carry out photosynthesis, is realized by the irradiation of light
Autotrophy provides enough nutrients for its breeding；
S8: after the density of chlorella reaches standard density in flask in S7 step, by the bead after being cultivated in S7 step
It is in eight hectoliters of reactor tank to continue growing operation and supplemental oxygen, carbon dioxide and be subject to light that algae, which is seeded to volume,
Irradiation；Guarantee that its photosynthetic is normally carried out by increasing carbon dioxide content；
S9: small after being cultivated in S8 step after the density of chlorella reaches standard density in reactor tank in S8 step
Ball algae, which is divided into 20 parts and is seeded in the reactor tank that 20 independent volumes are eight hectoliters, continues growing operation
And supplemental oxygen, carbon dioxide and it is subject to light-illuminating, S5 is inoculated with into S9 operates filter screen precision that water used uses as zero
002 millimeter of point, by carrying out high-precision filtering with water to inoculation, so that impurities in water particle greatly reduces.
S10: taking bead algae solution in S9 step in reactor tank to carry out Density Detection, and alga cells Board Lot is big when detection
It is as up to standard in 2,000,000 every milliliter；
S11: the reactor tank of the compound standard of density takes out the chlorella in it, and the chlorella in S11 in reactor tank is first led to
Flocculation concentration sedimentation is crossed, then drying is concentrated into small size, is finally centrifuged acquisition again, settles by flocculation concentration, then dries
It is concentrated into small size, finally carries out centrifugally operated again, realizes the quick obtaining to chlorella in reactor tank；
S12: the chlorella powder obtained in S11 is broken into powdery, is added in animal feed.
Working principle: the chlorella animal feed production technology when in use, by the progress of S1 to S9 step, so that algae
Class cell is able to carry out a large amount of breeding, after meeting the standard of Board Lot, is settled by flocculation concentration, then drying concentration
To small size, centrifugally operated is finally carried out again, realizes that the chlorella of acquisition is again by powder to the quick obtaining of chlorella in reactor tank
It is broken into powdery, is added in animal feed；By above-mentioned production technology, the production efficiency of chlorella is improved, and ensure that
The output quality of chlorella.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (7)
1. a kind of chlorella animal feed production technology, it is characterised in that the step of production technology is as follows:
S1: the appliance device in workshop is carried out disinfection processing；
S2: production culture medium, and chlorella mother cell is placed on it and is cultivated；
S3: the chlorella cells on culture medium in S2 are screened；
S4: the chlorella cells after screening in S3 are put into test tube, carry out test tube cultivation；
S5: the chlorella after cultivating in S4 step is seeded in the flask that capacity is 100 milliliters and continues growing operation simultaneously
It is subject to light-illuminating；
S6: after the density of chlorella reaches standard density in flask in S5 step, the chlorella after cultivating in S5 step is connect
Continue growing operation in the flask that kind is 1,000 milliliters to capacity and is subject to light-illuminating；
S7: after the density of chlorella reaches standard density in flask in S6 step, the chlorella after cultivating in S6 step is connect
Continue growing operation in the flask that kind is 5,000 milliliters to capacity and is subject to light-illuminating；
S8: after the density of chlorella reaches standard density in flask in S7 step, the chlorella after cultivating in S7 step is connect
It kind is in eight hectoliters of reactor tank to continue growing operation and supplemental oxygen, carbon dioxide and be subject to light and shine to volume
It penetrates；
S9: after the density of chlorella reaches standard density in reactor tank in S8 step, by the chlorella after being cultivated in S8 step
It is divided into 20 parts and is seeded to and continue growing operation in the reactor tank that 20 independent volumes are eight hectoliters and mend
It is oxygenated, carbon dioxide and is subject to light-illuminating；
S10: the bead algae solution in S9 step in reactor tank is taken to carry out Density Detection；
S11: the reactor tank of the compound standard of density takes out the chlorella in it；
S12: the chlorella powder obtained in S11 is broken into powdery, is added in animal feed.
2. a kind of chlorella animal feed production technology according to claim 1, it is characterised in that: in the workshop
Appliance device include flask, reactor tank, laboratory and workshop sterilizer.
3. a kind of chlorella animal feed production technology according to claim 2, it is characterised in that: the workshop sterilization disappears
Malicious equipment uses ultraviolet radiation for sterilizing and disinfecting.
4. a kind of chlorella animal feed production technology according to claim 1, it is characterised in that: the S5 connects into S9
The filter screen precision that kind operation water used uses is 0. 002 millimeter.
5. a kind of chlorella animal feed production technology according to claim 1, it is characterised in that: screened in the S3
Chlorella of the preferred diameter range in three to five micron diameter sizes in journey.
6. a kind of chlorella animal feed production technology according to claim 1, it is characterised in that: reacted in the S11
Chlorella in tank first passes through flocculation concentration sedimentation, and then drying is concentrated into small size, is finally centrifuged acquisition again.
7. a kind of chlorella animal feed production technology according to claim 1, it is characterised in that: the workshop sterilization disappears
Malicious equipment uses ozone sterilization.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101280328A (en) * | 2008-05-27 | 2008-10-08 | 清华大学 | Method for producing biodiesel by autotrophic culture and heterotrophic culture of chlorella |
| CN103881915A (en) * | 2012-12-20 | 2014-06-25 | 青岛中仁药业有限公司 | Method for producing chlorella for feeding by using livestock excrement |
| CN104498362A (en) * | 2014-12-26 | 2015-04-08 | 甘肃德福生物科技有限公司 | Biomass cultivation method for chlorella |
| CN106520559A (en) * | 2016-12-28 | 2017-03-22 | 海南绿藻世界生物科技有限公司 | High-efficiency light autotrophic culture method for chlorella |
-
2019
- 2019-06-14 CN CN201910516916.5A patent/CN110218653A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101280328A (en) * | 2008-05-27 | 2008-10-08 | 清华大学 | Method for producing biodiesel by autotrophic culture and heterotrophic culture of chlorella |
| CN103881915A (en) * | 2012-12-20 | 2014-06-25 | 青岛中仁药业有限公司 | Method for producing chlorella for feeding by using livestock excrement |
| CN104498362A (en) * | 2014-12-26 | 2015-04-08 | 甘肃德福生物科技有限公司 | Biomass cultivation method for chlorella |
| CN106520559A (en) * | 2016-12-28 | 2017-03-22 | 海南绿藻世界生物科技有限公司 | High-efficiency light autotrophic culture method for chlorella |
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Application publication date: 20190910 |