CN103243046A - Growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and using method of growth promoting agent - Google Patents
Growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and using method of growth promoting agent Download PDFInfo
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Abstract
The invention relates to a growth promoting agent for promoting photosynthetic bacteria strain to quickly breed and a using method of the growth promoting agent. The growth prompting agent is obtained as follows: adding 1 part weight of one or a mixture of sorbic acid, potassium sorbate or sodium sorbate to 1-4 parts by weight of alcohol for stirring, mixing and dissolving. The using method of the growth promoting agent is as follows: adding the growth promoting agent to a prepared photosynthetic bacteria liquid medium for stirring until the growth promoting agent is sufficiently dissolved; adding active photosynthetic bacteria strains for filling to a sealed or semi-open light transmission container for culturing at 25-32 DEG C by radiating under 1500lux-1600lux, wherein sorbate radial concentration of the growth promoting agent, which is added to the photosynthetic bacteria liquid medium, is controlled within a range of 0.005 mmol/L-1.0 mmol/L. The growth promoting agent for promoting photosynthetic bacteria strain to quickly breed is simple to use, less in usage, low in cost and capable of promoting photosynthetic bacteria strain to quickly breed. Moreover, the using method of the growth promoting agent is safe, ecological and environment-friendly and extensive in market prospect.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to a kind of photosynthetic bacterium bacterial strain growth promoter and using method thereof of breeding fast impelled.
Background technology
The ecological distribution of photosynthetic bacterium (Photosynthetic Bacteria is called for short PSB) is very extensive, mainly is distributed in ocean, rivers, lakes and marhshes, active sludge and the soil, and be that a class is aquatic, the hydrosphere microorganism.Photosynthetic bacterium with sunlight as the energy, be carbon source and nitrogenous source with multiple organism such as sodium-acetate, Sodium.alpha.-hydroxypropionate, N.F,USP MANNITOL, oxysuccinic acid, citric acid, glucose, glycerine, characteristics such as have that growth and breeding speed is fast, substratum wide material sources, meta-bolites are nutritious.Photosynthetic bacterial thallus contains rich in protein, amino acid, biological essential VITAMIN, the antiviral activity factor, Coenzyme Q10 99.0 and several physiological active substances, can provide organism necessary nutrition, promote animal and plant growth, strengthen its disease-resistant function, reduce the generation of disease, improve biological survival rate.Its product can be widely used in all many-sides such as fodder industry, agriculture production, environment protection, more and more is subjected to scientific worker and business people and payes attention to.
Photosynthetic bacterium product important index is somatic cells quantity and purity.Existing photosynthetic bacterium production technique mostly adopts open or semi open model liquid illumination cultivation, suppresses varied bacteria growing by the culture environment of improving substratum nutritive ingredient, increase intensity of illumination, the pH value that improves, the relative anaerobism of creation.Yet too high pH value can suppress the growth of photosynthetic bacterium itself, makes final somatic cells quantity on the low side; And be that raw material (substratum) is cultivated in the process of photosynthetic bacterium with the industrial and agricultural wastewater, because water body is subacidity mostly, consume not only cost height of a large amount of alkali lye heightening pH value, and contaminate environment, in this little acid or neutral aerobic environment, the infection of assorted bacterium also will be inevitable, be unfavorable for the foundation of photosynthetic bacterium dominant population.In a word, existing photosynthetic bacterium production technique can not satisfy the requirement of production application far away aspect strain growth speed, the somatic cells quantity that contains, thalline purity.
Summary of the invention
The present invention is for solving the problems of the prior art, provides a kind of and can impel the quick growth and breeding of photosynthetic bacterium bacterial strain, improves growth promoter and the using method thereof of somatic cells quantity greatly.
The present invention is achieved by the following technical solutions:
A kind of photosynthetic bacterium bacterial strain growth promoter of breeding fast that impels, described growth promoter is: by weight than with a kind of or mixture in 1 part of Sorbic Acid, potassium sorbate or sodium sorbate, join in 1-4 part ethanol, stirring, mixed dissolution are handled, and obtain this product growth promoter;
The temperature that described stirring, mixed dissolution are handled is-10～45 ℃, and the time is 1～10min;
Described ethanol is medical 75-95% ethanol.
A kind of photosynthetic bacterium bacterial strain using method of the growth promoter of breeding fast of impelling:
Growth promoter is joined in the photosynthetic bacterium liquid nutrient medium for preparing, be stirred to abundant dissolving, insert the photosynthetic bacterium bacterial classification of the activation of 10-30%, in the airtight or semi-open light transmission container of packing into, under 25～32 ℃, illumination cultivation;
The concentration that adds the sorb acid group of growth promoter in the described photosynthetic bacterium liquid nutrient medium is controlled in 0.005～1.0mmol/L scope.
Preferably, the concentration of the sorb acid group of adding growth promoter is in the described photosynthetic bacterium liquid nutrient medium: 0.01～0.5mmol/L.Be more preferably: 0.03～0.1mmol/L.
Described photosynthetic bacterium liquid nutrient medium refers to city organic industrial sewage, agricultural byproducts waste or the industrial waste of laboratory seed fermentation substratum commonly used or comprehensive treating process in the usual way.
Described laboratory seed fermentation substratum commonly used is composed as follows:
Ammonium chloride 1.0g, sodium-acetate 1.0g, sal epsom 0.2g, sodium bicarbonate 1.0g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.2g, ironic citrate 0.005g, yeast extract paste 1.0g, peptone 1.0g, inorganic salts solution 10mL, growth cofactor 0.01g, distilled water 1000mL, PH=6.5～8.0;
The cofactor of wherein growing comprises: one or more materials in VITMAIN B1, amino-benzene methyl alcohol, the vitamin H;
Wherein inorganic salts solution refers to: contain ironic citrate 5mg, calcium sulfate 0.05mg, boric acid 1mg, manganous chloride 0.05mg, zinc sulfate 1mg in every liter of distilled water.
The preparation method of the photosynthetic bacterium bacterial classification of described activation is:
With the photosynthetic bacterium laboratory for preparing seed fermentation substratum commonly used, pack in the transparent glass anaerobism bottle of 150ml capacity, through 115 ℃ of autoclavings 30 minutes, after the cooling, strict aseptic technique inserts Rhodopseudomonas palustris Rhodopeudomonas palustris bacterial classification, culture presevation CGMCC1.2180,1500-6000lux illumination cultivation 3-5 days, makes the photosynthetic bacterium bacterial classification of activation by culture temperature 28-32 ℃.
Culture presevation number is the bacterial classification that national DSMZ buys for the Rhodopseudomonas palustris Rhodopeudomonas palustris bacterial classification of CGMCC1.2180.
The present invention compared with prior art has following significant advantage:
1. promote that photosynthetic bacterium breeds fast.Under identical adaptation growth conditions, use the present invention and make the photosynthetic bacterium growth breeding fast, more do not add this photosynthetic bacterium promotor reproduction speed fast 1～2 day, improve product somatic cells concentration greatly.
2. addition is few, uses safety.This growth promoter that impels the photosynthetic bacterium bacterial strain to breed fast belongs to the additive that allows use in the food, and addition is few, belongs to safe interpolation scope, can be used for all many-sides such as agricultural, environmental protection, medicine.
3. culture medium raw material wide material sources of Shi Yonging.The culture medium raw material that the present invention is suitable for can be laboratory seed culture medium commonly used; When producing photosynthetic bacterium in enormous quantities, can the city organic industrial sewage, industrial waste, agricultural byproducts waste be raw material after comprehensive treating process, carry out fermentation culture after inoculating photosynthetic bacterium; Using at aspects such as pharmaceutical sectors, can analytical pure inorganic salts chemical reagent be raw material, makes substratum, inoculates, cultivates, purifies, makes the photosynthetic bacterium product.
4. inhibition varied bacteria growing.Gas spoilage organism growth that Sorbic Acid can mould fungus inhibition, yeast is become reconciled, and do not suppress photosynthetic bacterium growth, especially under acidic conditions, the Sorbic Acid of 0.02mmol/L can play bacteriostatic action; Be that raw material carries out photosynthetic bacterium when cultivating with the organic waste water of handling by fermentation, can suppress varied bacteria growing, promote photosynthetic bacterium growth, make photosynthetic bacterium become dominant microflora, the effect of bring into play bigger water purification or accumulating active substance.
5. thalline adaptability is good.The present invention all has growth promoting function to commercially available multiple photosynthetic bacterium bacterial strain, and liquid nutrient medium is not had particular requirement, uses safety, to eco-friendly.
Description of drawings
Fig. 1 is that different concns photosynthetic bacterium growth promoter is to the promoter action figure of photosynthetic bacterium growth
Embodiment
Specific embodiments of the present invention is described in detail with reference to the accompanying drawings.
Embodiment 1
Follow these steps to operation:
(1) photosynthetic bacterium actication of culture (indoor):
Preparation photosynthetic bacterium laboratory seed fermentation substratum commonly used is in the transparent glass anaerobism bottle of the 150ml capacity of packing into, through 115 ℃ of autoclavings 30 minutes.After the cooling, strict aseptic technique, insert Rhodopseudomonas palustris (the Rhodopeudomonas palustris culture presevation CGMCC1.2180) bacterial classification of buying, cultivated 3～5 days according to carrying out continuous illumination under the 2500lux at incandescent light, culture temperature 28-32 ℃, make the photosynthetic bacterium bacterial classification through overactivation.Test under microscope photosynthetic bacterial thallus stalwartness, motion are vivaciously, and be pollution-free.
The composed as follows of seed fermentation substratum used in described laboratory always:
Ammonium chloride 1.0g, sodium-acetate 1.0g, sal epsom 0.2g, sodium bicarbonate 1.0g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.2g, ironic citrate 0.005g, yeast extract paste 1.0g, peptone 1.0g, inorganic salts solution 10mL, growth cofactor 0.01g, distilled water 1000mL, PH=7.0.
The cofactor of wherein growing comprises: microbial culture such as VITMAIN B1, amino-benzene methyl alcohol, vitamin H are used material always.
Wherein inorganic salts solution refers to: the inorganic salts solution of trace, contain ironic citrate 5mg, calcium sulfate 0.05mg, boric acid 1mg, manganous chloride 0.05mg, zinc sulfate 1mg in every liter of distilled water.
(2) different concns photosynthetic bacterium growth promoter is to the promoter action (indoor) of photosynthetic bacterium growth:
Under laboratory condition, carry out the photosynthetic bacterium growth promoter and add the preferred of concentration.Preparation contains the photosynthetic bacterium laboratory seed fermentation substratum commonly used of different concns photosynthetic bacterium growth promoter, adds respectively in the transparent glass anaerobism bottle of 250ml capacity, and loading amount is 180ml, through 115 ℃ of autoclavings 30 minutes, cools off standby.
The one-tenth of different concns photosynthetic bacterium growth promoter is grouped into sees design table 1, establish 11 processing altogether, be numbered 1,2,3,4,5,6,7,8,9,10, the 11(contrast), the photosynthetic bacterium bacterial classification that activation is good is according to 10% inoculum size, be linked in the seed fermentation substratum commonly used of the good laboratory of containing the photosynthetic bacterium growth promoter of cooling, cultivated (temperature 28-32 ℃) 4 days at incandescent light according to carrying out continuous illumination under the 2500lux, measure photosynthetic bacterium bacterium number.
Table 1
Different concns photosynthetic bacterium growth promoter compound method is in the test:
The Sorbic Acid of calculated amount and ethanol are put into beaker shown in the weighing table 1, stir 3 minutes under the room temperature, and mixed dissolution adds in the seed fermentation substratum commonly used of photosynthetic bacterium laboratory, fully stirring and dissolving.
Test-results: as table 2, shown in Figure 1.
Fig. 1 be different concns photosynthetic bacterium growth promoter to the promoter action figure of photosynthetic bacterium growth, ordinate zou is that (hundred million/ml), X-coordinate is different photosynthetic bacterium concentration to the bacterium number.
Table 2
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | The 11(contrast) |
| Bacterium number (hundred million/ml) | 16.2 | 16.8 | 18.7 | 19.8 | 20.3 | 19.8 | 19.3 | 17.7 | 16.8 | 16.6 | 15.1 |
Test-results (table 2) shows, test the growth that each concentration Sorbic Acid and alcohol mixture all can promote photosynthetic bacterium, wherein the higher span of bacterium number is 0.01～0.5mmol/L(3～No. 8 processing), the span that the bacterium number is higher is 0.03～0.1mmol/L(4～No. 7 processing), it is the highest wherein to handle adding additive capacity (0.05mmol/L) bacterium numbers with No. 5.
Embodiment 2
Can follow these steps to operation to the present invention:
(1) photosynthetic bacterium actication of culture: with embodiment 1 (1);
(2) method is with embodiment 1 (2).
Difference is that the one-tenth of the photosynthetic bacterium growth promoter of adding is grouped into sees design table 3,
Add Different concentrations of alcohol (its add-on is seen design table 3), establish 7 processing altogether, be numbered 1,2,3,4,5,6, the 7(contrast).
Table 3
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Sorbic Acid (mmol/L) | 0.05 | 0.08 | 0 | 0 | 0 | 0 | 0 |
| Potassium sorbate (mmol/L) | 0 | 0 | 0.05 | 0.08 | 0 | 0 | 0 |
| Sodium sorbate (mmol/L) | 0 | 0 | 0 | 0 | 0.05 | 0.08 | 0 |
| Ethanol (mmol/L) | 0.15 | 0.20 | 0.15 | 0.20 | 0.15 | 0.20 | 0 |
Test-results: as shown in table 4.
Test-results shows, adds the mixture of Sorbic Acid, potassium sorbate, sodium sorbate and the ethanol of 0.05mmol/L or 0.08mmol/L in the seed culture medium commonly used of laboratory respectively, all can promote the growth of photosynthetic bacterium, improves photosynthetic bacterium bacterium number.
Table 4
| Numbering | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| Bacterium number (hundred million/ml) | 19.7 | 19.2 | 19.9 | 20.5 | 20.4 | 20.8 | 16.3 |
Embodiment 3
Can follow these steps to operation to the present invention:
(1) photosynthetic bacterium actication of culture: with embodiment 1 (1);
(2) the photosynthetic bacterium growth promoter is cultivated the influence (under laboratory condition) to growth under different pH condition: working method is with embodiment 1 (2);
Difference is that the composition of the photosynthetic bacterium growth promoter that adds in the seed culture medium commonly used of laboratory consists of the Sorbic Acid of 0.05mmol/L and the ethanol of 0.2mmol/L, regulates the pH value and is respectively 6.5,7.0,7.5,8.0.
Test-results: cultivation results such as the table 5 of different pH value culture condition.
Table 5
| The pH value | 6.5 | 7.0 | 7.5 | 8.0 |
| Photosynthetic bacterium growth promoter (hundred million/ml) | 19.2 | 20.8 | 20.2 | 18.6 |
| Contrast (hundred million/ml) | 16.5 | 16.3 | 15.6 | 14.8 |
Test-results shows that the pH value is added the Sorbic Acid of 0.05mmol/L and the ethanol of 9mmol/L and all can be promoted photosynthetic bacterium growth in various degree, through 4 days cultivation, improves somatic cells concentration greatly in 6.5～8.0 scopes.
Embodiment 4
(1) photosynthetic bacterium actication of culture: with embodiment 1 (1);
(2) enlarge fermentation culture:
Conventional seed fermentation substratum (pH7.5) 10L in configuration laboratory, the mixture that adds 0.015g potassium sorbate (with respect to 0.1mmol/L) and 0.5ml ethanol, stirring and dissolving, method is with embodiment 1 (2), be contrast not add the conventional seed fermentation substratum in potassium sorbate and ethanol laboratory, by the photosynthetic bacterium bacterial classification of 30% inoculum size access through overactivation, the 5000 milliliters of transparent vials (also can adopt the white plastic bucket) of packing into interior (cultivating base unit weight is 70%), place irradiation cultivation (in the temperature 25-32 ℃ of scope) under the nature sunlight, 8 days expansion fermentation culture maturations.
The result shows: through 8 days cultivation, the photosynthetic bacterial thallus cell quantity reached more than 1,800,000,000/milliliter, adds the growth that Sorbic Acid can promote photosynthetic bacterium, and the control group bacterium number that does not more add Sorbic Acid is high by 17.8%.Test under microscope photosynthetic bacterium motion is active, the thalline stalwartness, and pollution-free, no abnormal phenomenon can be applicable to the fertilisings of crops such as vegetables or Chinese medicine, also can be used for livestock and poultry cultivation.
Embodiment 5
(1) photosynthetic bacterium actication of culture: with embodiment 1 (1);
(2) enlarge fermentation culture:
With photosynthetic bacterium seed fermentation nutrient solution (active photosynthetic bacterium number, every m1 reaches more than 1,000,000,000), by 20% be transferred to enlarge expand in the fermention medium numerous, this expansion is selected beer waste water for use with fermention medium, regulating the pH value with sodium hydroxide is 6.5～7.0, the dress beer waste water is 8 kilograms in 10 kilograms of white plastic buckets, and every liter adds the potassium sorbate of 0.1mmol/L and the mixture of 0.5ml ethanol, mixes dissolving.White plastic bucket after the switching can be placed under the nature solar irradiation to be cultivated, 10 days maturations, and its technical indicator can reach as table 6.
Table 6
Embodiment 6
Can follow these steps to operation to the present invention:
(1) photosynthetic bacterium actication of culture: with embodiment 1 (1);
(2) enlarge fermentation culture: photosynthetic bacterium seed fermentation nutrient solution (is contained active photosynthetic bacterium number, every m1 reaches 1,000,000,000), by 30% inoculum size be transferred to enlarge expand in the fermention medium numerous, this expansion fermention medium is selected pig farm organic waste water (pH7.0) for use, 7 kilograms of pig farm organic waste waters are housed in 10 liters of white plastic buckets, every liter of mixture that adds a certain amount of potassium sorbate (addition sees Table 7 in detail) and 1ml ethanol again, be placed on then under the nature solar irradiation and cultivated (25～33 ℃ of temperature) 10 days, its photosynthetic bacterium content can reach as following table 7.
Table 7
Test-results shows, adds the growth that potassium sorbate is conducive to photosynthetic bacterium under the complicated microbial environment.
All commercially available acquisitions of the photosynthetic bacterium bacterial classification that adopts in the embodiment of the invention there is no particular requirement to it.
If use soluble salts such as potassium sorbate, sodium sorbate, ethanol and sorbate directly can be added in the photosynthetic bacterium liquid nutrient medium in the test, be stirred to abundant dissolving.
The selection principle that enlarges fermention medium when the present invention uses is:
Use in agricultural, poultry industry, environmental protection industry, can the city organic industrial sewage, industrial waste, agricultural byproducts waste be raw material after comprehensive treating process, adds additive again; Using at aspects such as pharmaceutical sectors, should be raw material with analytical pure inorganic salts chemical reagent then, makes substratum, inoculation, cultivation.
Additive of the present invention is for the photosynthetic bacterium bacterial classification of advantage, and its effect is more remarkable.
Claims (7)
1. one kind is impelled the photosynthetic bacterium bacterial strain growth promoter of breeding fast, it is characterized in that, described growth promoter is: by weight than with a kind of or mixture in 1 part of Sorbic Acid, potassium sorbate or sodium sorbate, join in 1-4 part ethanol, stirring, mixed dissolution are handled, and obtain this product growth promoter;
The temperature that described stirring, mixed dissolution are handled is-10～45 ℃, and the time is 1～10min;
Described ethanol is medical 75-95% ethanol.
2. one kind is impelled the photosynthetic bacterium bacterial strain using method of the growth promoter of breeding fast, it is characterized in that,
Growth promoter is joined in the photosynthetic bacterium liquid nutrient medium for preparing, be stirred to abundant dissolving, insert the photosynthetic bacterium bacterial classification of the activation of 10-30%, in the airtight or semi-open light transmission container of packing into, under 25～32 ℃, the 1500-6000lux illumination cultivation;
The concentration that adds the sorb acid group of growth promoter in the described photosynthetic bacterium liquid nutrient medium is controlled in 0.005～1.0mmol/L scope.
3. a kind of photosynthetic bacterium bacterial strain using method of the growth promoter of breeding fast of impelling as claimed in claim 2 is characterized in that,
The concentration that adds the sorb acid group of growth promoter in the described photosynthetic bacterium liquid nutrient medium is: 0.01～0.5mmol/L.
4. a kind of photosynthetic bacterium bacterial strain using method of the growth promoter of breeding fast of impelling as claimed in claim 3 is characterized in that,
The concentration that adds the sorb acid group of growth promoter in the described photosynthetic bacterium liquid nutrient medium is: 0.03～0.1mmol/L.
5. a kind of photosynthetic bacterium bacterial strain using method of the growth promoter of breeding fast of impelling as claimed in claim 2 is characterized in that,
Described photosynthetic bacterium liquid nutrient medium refers to city organic industrial sewage, agricultural byproducts waste or the industrial waste of laboratory seed fermentation substratum commonly used or comprehensive treating process in the usual way.
6. a kind of using method of impelling the growth promoter of the quick breeding of photosynthetic bacterium bacterial strain as claimed in claim 5 is characterized in that, described laboratory seed fermentation substratum commonly used is composed as follows:
Ammonium chloride 1.0g, sodium-acetate 1.0g, sal epsom 0.2g, sodium bicarbonate 1.0g, sodium-chlor 0.5g, dipotassium hydrogen phosphate 0.2g, ironic citrate 0.005g, yeast extract paste 1.0g, peptone 1.0g, inorganic salts solution 10mL, growth cofactor 0.01g, distilled water 1000mL, PH=6.5～8.0;
The cofactor of wherein growing comprises: one or more materials in VITMAIN B1, amino-benzene methyl alcohol, the vitamin H;
Wherein inorganic salts solution refers to: contain ironic citrate 5mg, calcium sulfate 0.05mg, boric acid 1mg, manganous chloride 0.05mg, zinc sulfate 1mg in every liter of distilled water.
7. a kind of fast using method of the growth promoter of breeding of photosynthetic bacterium bacterial strain of impelling as claimed in claim 2 is characterized in that the preparation method of the photosynthetic bacterium bacterial classification of described activation is:
With the photosynthetic bacterium laboratory for preparing seed fermentation substratum commonly used, pack in the transparent glass anaerobism bottle of 150ml capacity, through 115 ℃ of autoclavings 30 minutes, after the cooling, strict aseptic technique inserts Rhodopseudomonas palustris Rhodopeudomonas palustris bacterial classification, culture presevation number is CGMCC1.2180,1500-6000lux illumination cultivation 3-5 days, makes the photosynthetic bacterium bacterial classification of activation by culture temperature 28-32 ℃.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104628429A (en) * | 2015-03-04 | 2015-05-20 | 蒋常德 | Preparation method of photosynthetic bacterial manure |
| CN108484237A (en) * | 2018-04-28 | 2018-09-04 | 沈阳师范大学 | A kind of preparation method and application of agricultural photosynthetic bacteria microorganism fertilizer |
| CN108587975A (en) * | 2018-05-16 | 2018-09-28 | 广西青又青生物肥业有限公司 | A kind of Rhodopseudomonas palustris culture medium and cultural method |
| CN110468087A (en) * | 2019-09-24 | 2019-11-19 | 中国热带农业科学院热带生物技术研究所 | A kind of photosynthetic bacteria culture medium and preparation method thereof simply easily made |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1414092A (en) * | 2002-09-30 | 2003-04-30 | 中国科学院沈阳应用生态研究所 | Additive for photosyntheric bacterial culture medium |
| CN102992894A (en) * | 2012-11-20 | 2013-03-27 | 蒋常德 | Preparation method for high-concentration photosynthesis bacterial manure |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1414092A (en) * | 2002-09-30 | 2003-04-30 | 中国科学院沈阳应用生态研究所 | Additive for photosyntheric bacterial culture medium |
| CN102992894A (en) * | 2012-11-20 | 2013-03-27 | 蒋常德 | Preparation method for high-concentration photosynthesis bacterial manure |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104628429A (en) * | 2015-03-04 | 2015-05-20 | 蒋常德 | Preparation method of photosynthetic bacterial manure |
| CN108484237A (en) * | 2018-04-28 | 2018-09-04 | 沈阳师范大学 | A kind of preparation method and application of agricultural photosynthetic bacteria microorganism fertilizer |
| CN108587975A (en) * | 2018-05-16 | 2018-09-28 | 广西青又青生物肥业有限公司 | A kind of Rhodopseudomonas palustris culture medium and cultural method |
| CN110468087A (en) * | 2019-09-24 | 2019-11-19 | 中国热带农业科学院热带生物技术研究所 | A kind of photosynthetic bacteria culture medium and preparation method thereof simply easily made |
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