CN102311920A - Culture method for chlorella - Google Patents

Culture method for chlorella Download PDF

Info

Publication number
CN102311920A
CN102311920A CN2010102209066A CN201010220906A CN102311920A CN 102311920 A CN102311920 A CN 102311920A CN 2010102209066 A CN2010102209066 A CN 2010102209066A CN 201010220906 A CN201010220906 A CN 201010220906A CN 102311920 A CN102311920 A CN 102311920A
Authority
CN
China
Prior art keywords
days
culture
chlorella
gas
temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN2010102209066A
Other languages
Chinese (zh)
Other versions
CN102311920B (en
Inventor
王领民
金平
师文静
李晓姝
王崇辉
佟明友
黎元生
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
China Petroleum and Chemical Corp
Sinopec Fushun Research Institute of Petroleum and Petrochemicals
Original Assignee
China Petroleum and Chemical Corp
Sinopec Fushun Research Institute of Petroleum and Petrochemicals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by China Petroleum and Chemical Corp, Sinopec Fushun Research Institute of Petroleum and Petrochemicals filed Critical China Petroleum and Chemical Corp
Priority to CN 201010220906 priority Critical patent/CN102311920B/en
Publication of CN102311920A publication Critical patent/CN102311920A/en
Application granted granted Critical
Publication of CN102311920B publication Critical patent/CN102311920B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Landscapes

  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

The invention discloses a culture method for chlorella, which comprises the following contents: the volume of chlorella seed liquid in a bioreactor accounts for 5 to 15 percent of the total volume of a culture medium; a gas containing 5 to 15 volume percent of CO2 is introduced, the volume of introduced gas is 0.1 to 1.0vvm; and the culture temperature is reduced to 0 to 15 DEG C from 25 to 40 DEG C within 0.5 to 10 days, and chlorella is continuously cultured at a constant temperature of 0 to 15 DEG C for 5 to 15 days. The method disclosed by the invention can allow microalgae to grow normally at a low temperature, and the accumulated amount of fat and CO2 utilization rate of microalgae can be increased.

Description

The cultural method of a kind of chlorella
Technical field
The invention belongs to little algae biological technical field, relate to the cultural method of a kind of chlorella.
Background technology
Nutritive ingredients (like tenaculat Habenaria) such as little algae rich in proteins, polysaccharide, unsaturated fatty acids can be used for food, medicine and energy aspect; Can accumulate lipid acid in a large number, having slightly, the algae fatty acid content can account for 30%～60% of dry weight.Utilize culturing microalgae to accumulate oil resource, become the research field of utilizing the solar energy development renewable resources the most popular at present.Not only have powerful market potential, and have outstanding social value.
But most of wild micro algae growth speed is unhappy, and the stabilizing carbon dioxide ability is also limited, accomplish scale production, and needs to consume lot of manpower and material resources, and gets little.Current, little algae research is on the occasion of popular period, and the speed of growth of little algae and oil and fat accumulation amount become the emphasis of research.
The production of little algae will consume CO 2, CO 2Effective utilization absorb, be the key of culture effect of realizing ideal, in the open at present photo bio culturing microalgae technology, many CO are arranged 2Supply mode and device." supplementing CO 2 to micro-algae culture pond device " (application numbers 200610018771.9) such as Lee's noctilucence adopt a kind of CO that links to each other with the breed pond 2Device for supplying can effectively improve CO 2Utilization ratio, but technology is loaded down with trivial details, has increased facility investment." a kind of little algae industrial production is used the photosynthetic organism reactor assembly " (application numbers 03128138.9) such as Liu Jianguo etc. " the pipeline bioreactor that microalgae mass is cultivated " (application number 200410020978.0) and Miao JianRens all is employed in and adds a kind of installation method in the photo-bioreactor system, realizes CO 2Supply, can realize also that simultaneously certain oxygen resolves effect.These all increase facility investment unavoidably, and technological process is loaded down with trivial details.Also has other a kind of CO in the bibliographical information at present 2Supply mode, Cong Wei etc. use stack gas to mend carbon at " mending the method for carbon culturing microalgae through the pH value feedback control " (application number 200410009360.4) and " carbon compensator and the method for use thereof that are used for extensive little algae are share the way " (application number 200510126465.2).
In the prior art, through CO 2Device for supplying is realized higher CO 2Concentration of ordinary dissolution improves CO 2Utilising efficiency.In addition, culture temperature also is to influence CO 2An important factor of concentration of ordinary dissolution.General little algae is cultivated its CO under 25 ℃～35 ℃ conditions at present 2Solubleness is more or less the same, when culture temperature drops to after 5 ℃～15 ℃ CO 2Solubleness in nutrient solution has improved 3～4 times, and this provides priority condition for the cultivation of little algae.But the temperature reduction can have a strong impact on the speed of growth of little algae, even most of little algae can't be grown, and therefore, rarely has report about culturing microalgae at low temperatures in the prior art.
Summary of the invention
To the deficiency of prior art, the present invention provides a kind of little algae high-efficient culture method.The inventive method can make little algae normal growth at low temperatures, improves microalgae grease accumulation volume and CO 2Utilising efficiency.
Microalgae culture method of the present invention comprises following content: the per-cent that the interior algae seedling solution volume of bio-reactor accounts for the substratum TV is 5-15%, feeds CO 2Content is the gas of 5v%-15v%; Air flow is 0.1-1.0vvm (PM feeds the unit volume amount in the unit volume volume); PH is 7-10 between during cultivation, and culture temperature was cooled to 0 ℃-15 ℃ from 25 ℃-40 ℃ in 0.5-10 days, continues constant temperature culture 5-15 days down in 0 ℃-15 ℃.
The inventive method culture temperature can at the uniform velocity be lowered the temperature 1-10 ℃ in every 0.5-5 days from 25 ℃-40 ℃, whenever fell 1-10 ℃ of constant temperature culture 0.5-5 days, until being cooled to 0-15 ℃, also can in 0.5-10 days, at the uniform velocity be cooled to 0 ℃-15 ℃ from 25 ℃-40 ℃.
Stirring velocity is 80-100rpm in the described bio-reactor of the inventive method, and intensity of illumination is 1000-60001x.
The inventive method algae kind is a kind of chlorella, comprises Chlorella vulgaris on the market, also can comprise through carrying out the heterotrophism heterophytic chlorella of growth fast after the domestication.
The essentially consist of the used substratum of the inventive method is following:
NaNO 3Or KNO 3 0.10～0.50g
K 2HPO 4·3H 2O 0.05～0.10g
MgSO 4·7H 2O 0.05～0.10g
CaCl 2·2H 2O 0.01～0.05g
KH 2PO 4 0.05～0.5g
NaCl 0.01～0.05g
Soil?extract *(soil extract) 10～40mL
FeCl 3·6H 2O 0.001～0.005g
Fe-EDTA 0.5～2mL
Water 1L
The inventive method can also add the Na of 0.01～0.05mmol/L concentration in the chlorella substratum 2SO 3Micro-mixed solution with 0.5～1.0mL/L.Above-mentioned every liter of micro-mixed solution composition is following: H 3BO 35.5～6.5g, ZnSO 47H 2O 4.0～5.0g, MnCl 2H 2O 0.5～1.0g, (NH 4) 6Mo 7O 244H 2O0.20～0.40g, CuSO 45H 2O 0.50～1.00g, Co (NO 3) 26H 2O 0.10～0.20g.
In the inventive method, feeding contain CO 2The gas carbon dioxide containing gas that can obtain from any fermentation process; Preferably anaerobically fermenting or micro-aerobe fermentation process discharge tail gas; Tail gas for the aerobic microbiological fermenting process can carry out deoxidation treatment earlier, utilizes then, as adsorbing deoxidation treatment etc.; Oxygen level is preferably and is lower than 8% and (v), most preferably is and is lower than 5% (v).Typical anaerobic process such as Cray doctor pneumobacillus anaerobically fermenting produce 1, ammediol, the initial 99%N that feeds 2, thalline produces a large amount of CO at the anaerobic state bottom fermentation 2Gas contains CO in the general tail gas 2Volumetric concentration be about 10%.Typical aerobic processes such as saccharomycetes to make fermentation system alcoholic acid process, this tail gas can be used after through adsorbing and removing part oxygen.
Compared with prior art, the present invention's method of cultivating chlorella has following characteristics:
1, the synergy of the inventive method through suitable temperature control and suitable medium of selecting for use and culture condition makes the chlorella after the cultivation can be at 0-15 ℃ of following normal growth, and it is slow to have solved in the prior art micro algae growth, even the problem that can't grow.
2, the inventive method is because the chlorella turned out can be at 0-15 ℃ of following normal growth, contained CO in this moment nutrient solution 2Concentration increase greatly, improved CO 2Utilization ratio, particularly in substratum, contain certain density Na 2SO 3During with trace element, make that greasy accumulation volume obviously improves in the chlorella, be suitable as the raw material of biofuel.
3, the inventive method is guaranteeing CO 2Widened the suitable growth temperature of chlorella in the time of utilization ratio; Avoided in the chlorella breeding process to temperature controlling with culture the requirement of chlorella to region, time, for example at all seasons or all can carry out cultivating large area in the lower zone of northern China temperature.
Embodiment
Be further described below by the technical scheme of specific embodiment the inventive method.Among the inventive method embodiment, the concentration of described substance all is to be benchmark with the substratum TV, promptly the content of substance account for the nutrient solution preparation accomplish after total nutrient solution volume percent or mole number or quality etc.The algae kind of setting out is that nature separates the little algae of a kind of autotrophy that obtains, and separation and purification is accredited as Chlorella vulgaris through the laboratory, and the laboratory is numbered FY1#, and used substratum is a minimum medium, composition such as table 1:
Table 1
NaNO 3 0.25g
K 2HPO 4·3H 2O 0.075g
MgSO 4·7H 2O 0.075g
CaCl 2·2H 2O 0.025g
KH 2PO 4 0.175g
NaCl 0.025
Soil extract (soil extract) 40mL
FeCl 3·6H 2O 0.005g
Fe-EDTA 1mL
Zero(ppm) water 1000mL
Embodiment 1
The per-cent that the interior chlorella algae seedling solution volume of bio-reactor accounts for the substratum TV is 10%, and stirring velocity is 90rpm, and intensity of illumination is 30001x, feeds to contain CO 2Gas be anaerobically fermenting or micro-aerobe fermentation process discharge tail gas, CO 2Content is the gas of 10v%, and air flow is 0.5vvm, and pH is 7-10 between during cultivation, and culture temperature is at the uniform velocity lowered the temperature 5 ℃ from 30 ℃ of every days, whenever falls 5 ℃ of constant temperature culture 1 day, reduces to 0 ℃ up to temperature, continues constant temperature culture 10 days down in 0 ℃.Obtain some single algaes and fall, this single algae is fallen behind the picking, draw and in solid inclined-plane minimum medium, cultivate, after waiting to grow, can obtain purebred algae kind FY1T.The purebred algae kind of this chlorella is transferred to shake in the bottle under 5-15 ℃, 100rpm, 30001x illumination and airbath condition from the inclined-plane cultivates, obtain the experimental data under the autotrophy culture condition.Cultivate 10d, analytical test shakes the concentration of frustule in the bottle, measures viable count wherein with cell counter, with the total percentage of contained grease composition in the frustule under the soxhlet extraction analysis dry weight situation.
Embodiment 2
The per-cent that the interior chlorella algae seedling solution volume of bio-reactor accounts for the substratum TV is 15%, and stirring velocity is 100rpm, and intensity of illumination is 20001x, feeds to contain CO 2Gas be the gas after the tail gas deoxidation treatment of aerobic microbiological fermenting process, oxygen level is for being lower than 8v%, CO 2Content is the gas of 5v%, and air flow is 1.0vvm, and pH is 7-10 between during cultivation, and culture temperature at the uniform velocity is cooled to 0 ℃ in 6 days from 25 ℃, continues constant temperature culture 10 days down in 0 ℃.Obtain some single algaes and fall, this single algae is fallen behind the picking, draw and in solid inclined-plane minimum medium, cultivate, after waiting to grow, can obtain purebred algae kind FY1T.The purebred algae kind of this chlorella is transferred to shake in the bottle under 5-15 ℃, 100rpm, 30001x illumination and airbath condition from the inclined-plane cultivates, obtain the experimental data under the autotrophy culture condition.Cultivate 10d, analytical test shakes the concentration of frustule in the bottle, measures viable count wherein with cell counter, with the total percentage of contained grease composition in the frustule under the soxhlet extraction analysis dry weight situation.
Embodiment 3
The per-cent that the interior chlorella algae seedling solution volume of bio-reactor accounts for the substratum TV is 5%, in substratum, adds the Na of 0.02mmol/L 2SO 3With the trace element solution of 0.8mL/L, above-mentioned every liter of micro-mixed solution composition is following: H 3BO 36.0g, ZnSO 47H 2O 4.5g, MnCl 2H 2O 0.75g, (NH 4) 6Mo 7O 244H 2O 0.35g, CuSO 45H 2O 0.75g, Co (NO 3) 26H 2O 0.15g, stirring velocity is 80rpm, intensity of illumination is 30001x, feeds to contain CO 2Gas be anaerobically fermenting or micro-aerobe fermentation process discharge tail gas, CO 2Content is the gas of 15v%, and air flow is 0.1vvm, and pH is 7-10 between during cultivation, and culture temperature at the uniform velocity is cooled to 10 ℃ in 5 days from 35 ℃, continues constant temperature culture 10 days down in 10 ℃.Obtain some single algaes and fall, this single algae is fallen behind the picking, draw and in solid inclined-plane minimum medium, cultivate, after waiting to grow, can obtain purebred algae kind FY1T.The purebred algae kind of this chlorella is transferred to shake in the bottle under 5-15 ℃, 100rpm, 30001x illumination and airbath condition from the inclined-plane cultivates, obtain the experimental data under the autotrophy culture condition.Cultivate 10d, analytical test shakes the concentration of frustule in the bottle, measures viable count wherein with cell counter, with the total percentage of contained grease composition in the frustule under the soxhlet extraction analysis dry weight situation.
Comparative example 1
The algae kind of setting out is autotrophy chlorella FY1, the purebred algae kind of this chlorella is transferred to shake in the bottle under 25-30 ℃, 100rpm, 30001x illumination and airbath condition from the inclined-plane cultivates, and obtains the experimental data under the autotrophy culture condition.Cultivate 10d, analytical test shakes the concentration of frustule in the bottle, measures viable count wherein with cell counter, with the total percentage of contained grease composition in the frustule under the soxhlet extraction analysis dry weight situation.
Comparative example 2
The algae kind of setting out is autotrophy chlorella FY1, the purebred algae kind of this chlorella is transferred to shake in the bottle under 5-15 ℃, 100rpm, 30001x illumination and airbath condition from the inclined-plane cultivates, and obtains the experimental data under the autotrophy culture condition.Cultivate 10d, analytical test shakes the concentration of frustule in the bottle, measures viable count wherein with cell counter, with the total percentage of contained grease composition in the frustule under the soxhlet extraction analysis dry weight situation.
The foregoing description experimental result such as following table:
Wherein, the relation of the conversion typical curve between the cell concn (y) that records of dry weight (x) and cell counting is as follows:
y＝0.6856x×10 8-2.68×10 6
The algae dry weight Fat content Frustule concentration (* 10 8
(g/L) (％) Individual/mL)
Embodiment 1 1.18 37.8 0.7822
Embodiment 2 1.06 38.5 0.6999
Embodiment 3 1.14 40.1 0.7547
Comparative example 1 1.05 17.9 0.6931
Comparative example 2 0.16 10.2 0.0829
Can know that from above-mentioned experimental result embodiment 1-3 is the chlorella algae kind of the synergy of suitable temperature control and suitable medium of selecting for use and culture condition after cultivating, 4-5 be a kind of autotrophy chlorella bath kind of separating acquisition from nature.Both compare, the inventive method obtain chlorella algae kind not only can be in lower temperature range normal growth, and its solid carbon is satisfactory for result, the fat content that accumulates is more than two times of oil and fat accumulation amount of algae kind of setting out.

Claims (9)

1. the cultural method of a chlorella is characterized in that comprising following content: to account for the per-cent of substratum TV be 5-15% to the algae seedling solution volume in the bio-reactor, feeds CO 2Content is the gas of 5v%-15v%, and air flow is 0.1-1.0vvm, and pH is 7-10 between during cultivation, and culture temperature was cooled to 0 ℃-15 ℃ from 25 ℃-40 ℃ in 0.5-10 days, continues constant temperature culture 5-15 days down in 0 ℃-15 ℃.
2. cultural method as claimed in claim 1 is characterized in that: described culture temperature was at the uniform velocity lowered the temperature 1-10 ℃ in every 0.5-5 days from 25 ℃-40 ℃, whenever fell 1-10 ℃ of constant temperature culture 0.5-5 days, until being cooled to 0-15 ℃.
3. cultural method as claimed in claim 1 is characterized in that: described culture temperature at the uniform velocity was cooled to 0 ℃-15 ℃ from 25 ℃-40 ℃ in 0.5-10 days.
4. cultural method as claimed in claim 1 is characterized in that: stirring velocity is 80-100rpm in the described bio-reactor, and intensity of illumination is 1000-60001x.
5. cultural method as claimed in claim 1 is characterized in that: described substratum basic composition is the NaNO that contains 0.10～0.50g in every premium on currency 3Or KNO 3, the K of 0.05～0.10g 2HPO 43H 2O, the MgSO of 0.05～0.10g 47H 2O, the CaCl of 0.01～0.05g 22H 2O, the KH of 0.05～0.5g 2PO 4, the NaCl of 0.01～0.05g, the soil extract of 10～40mL, the FeCl of 0.001～0.005g 36H 2O, the Fe-EDTA of 0.5～2mL.
6. like claim 1 or 4 described cultural methods, it is characterized in that: described substratum contains the Na that concentration is 0.01～0.05mmol/L 2SO 3
7. like claim 1 or 4 described cultural methods, it is characterized in that: described substratum contains the micro-mixed solution that concentration is 0.5～1.0mL/L, and every liter of micro-mixed solution composition is H 3BO 35.5～6.5g, ZnSO 47H 2O 4.0～5.0g, MnCl 2H 2O 0.5～1.0g, (NH 4) 6Mo 7O 244H 2O0.20～0.40g, CuSO 45H 2O 0.50～1.00g, Co (NO 3) 26H 2O 0.10～0.20g.
8. the method for claim 1 is characterized in that: contain CO 2Gas be anaerobically fermenting or micro-aerobe fermentation process discharge tail gas.
9. the method for claim 1 is characterized in that: contain CO 2Gas be the gas after the tail gas deoxidation treatment of aerobic microbiological fermenting process, oxygen level is for being lower than 8v%.
CN 201010220906 2010-07-07 2010-07-07 Culture method for chlorella Active CN102311920B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201010220906 CN102311920B (en) 2010-07-07 2010-07-07 Culture method for chlorella

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201010220906 CN102311920B (en) 2010-07-07 2010-07-07 Culture method for chlorella

Publications (2)

Publication Number Publication Date
CN102311920A true CN102311920A (en) 2012-01-11
CN102311920B CN102311920B (en) 2013-08-28

Family

ID=45425487

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201010220906 Active CN102311920B (en) 2010-07-07 2010-07-07 Culture method for chlorella

Country Status (1)

Country Link
CN (1) CN102311920B (en)

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105087384A (en) * 2014-05-15 2015-11-25 中国石油化工股份有限公司 Method for culturing photosynthetic microbes and method for producing grease
CN105087412A (en) * 2014-05-15 2015-11-25 中国石油化工股份有限公司 Method for cultivating photosynthetic microorganisms and method for producing grease
CN105198635A (en) * 2015-10-22 2015-12-30 湛江恒兴南方海洋科技有限公司 Macro-element nutrient solution for large-scale culture of Chlorella salina
CN106701584A (en) * 2015-07-23 2017-05-24 中国石油化工股份有限公司 Microalgae harvesting method and application thereof
CN107198038A (en) * 2017-05-24 2017-09-26 甘肃凯源生物技术开发中心 The feed of cultivation health pig prepared by chlorella powder and spirulina powder composition and said composition
CN109722407A (en) * 2017-10-30 2019-05-07 北京林业大学 A kind of method of microalgae ice startup temperature compensatory growth
CN110129062A (en) * 2019-06-14 2019-08-16 阿尔格生命科学(江苏)有限公司 A kind of algae competent cell soil conditioning agent producing process
CN112694977A (en) * 2021-01-12 2021-04-23 曹礼刚 Chlorella culture method
WO2023043063A1 (en) * 2021-09-17 2023-03-23 씨제이제일제당 (주) Microalgae strain having effect of promoting plant growth and use thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1418946A (en) * 2002-11-05 2003-05-21 清华大学 Method for semiaseptic culturing heterotrophic chlorella
CN1702167A (en) * 2005-02-28 2005-11-30 张小葵 Culture method for sea micro-algae
CN1724637A (en) * 2004-07-21 2006-01-25 中国科学院过程工程研究所 Mend the method that carbon is cultivated little algae by pH value feedback control
CN1982432A (en) * 2005-12-12 2007-06-20 中国科学院过程工程研究所 Carbon compensator for large-scale culturing micro-algae, its utilization and use

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1418946A (en) * 2002-11-05 2003-05-21 清华大学 Method for semiaseptic culturing heterotrophic chlorella
CN1724637A (en) * 2004-07-21 2006-01-25 中国科学院过程工程研究所 Mend the method that carbon is cultivated little algae by pH value feedback control
CN1702167A (en) * 2005-02-28 2005-11-30 张小葵 Culture method for sea micro-algae
CN1982432A (en) * 2005-12-12 2007-06-20 中国科学院过程工程研究所 Carbon compensator for large-scale culturing micro-algae, its utilization and use

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
周韬等: "耐低温杜氏藻突变株的诱变和鉴定", 《台湾海峡》, 30 November 2006 (2006-11-30), pages 498 - 500 *
栗淑媛: "低温胁迫对螺旋藻脱氢酶活性的影响", 《微生物学通报》, 31 December 2005 (2005-12-31), pages 47 - 50 *
王妮等: "耐低温螺旋藻新品系的诱变选育", 《安徽农业科学》, 31 December 2008 (2008-12-31), pages 12552 - 12553 *

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105087412B (en) * 2014-05-15 2018-02-23 中国石油化工股份有限公司 Cultivate the method for photosynthetic microorganism and the method for production grease
CN105087412A (en) * 2014-05-15 2015-11-25 中国石油化工股份有限公司 Method for cultivating photosynthetic microorganisms and method for producing grease
CN105087384B (en) * 2014-05-15 2018-02-23 中国石油化工股份有限公司 Cultivate the method for photosynthetic microorganism and the method for production grease
CN105087384A (en) * 2014-05-15 2015-11-25 中国石油化工股份有限公司 Method for culturing photosynthetic microbes and method for producing grease
CN106701584A (en) * 2015-07-23 2017-05-24 中国石油化工股份有限公司 Microalgae harvesting method and application thereof
CN106701584B (en) * 2015-07-23 2019-11-15 中国石油化工股份有限公司 A kind of microalgae recovery method and its application
CN105198635A (en) * 2015-10-22 2015-12-30 湛江恒兴南方海洋科技有限公司 Macro-element nutrient solution for large-scale culture of Chlorella salina
CN107198038A (en) * 2017-05-24 2017-09-26 甘肃凯源生物技术开发中心 The feed of cultivation health pig prepared by chlorella powder and spirulina powder composition and said composition
CN107198038B (en) * 2017-05-24 2021-01-15 河西学院 Chlorella powder and spirulina powder composition and feed prepared from composition and used for breeding healthy pigs
CN109722407A (en) * 2017-10-30 2019-05-07 北京林业大学 A kind of method of microalgae ice startup temperature compensatory growth
CN110129062A (en) * 2019-06-14 2019-08-16 阿尔格生命科学(江苏)有限公司 A kind of algae competent cell soil conditioning agent producing process
CN112694977A (en) * 2021-01-12 2021-04-23 曹礼刚 Chlorella culture method
WO2023043063A1 (en) * 2021-09-17 2023-03-23 씨제이제일제당 (주) Microalgae strain having effect of promoting plant growth and use thereof
TWI824648B (en) * 2021-09-17 2023-12-01 南韓商Ｃｊ第一製糖股份有限公司 Microalgae strain having an effect of promoting plant growth and use thereof

Also Published As

Publication number Publication date
CN102311920B (en) 2013-08-28

Similar Documents

Publication Publication Date Title
CN102311920B (en) Culture method for chlorella
Zhang et al. Vertical-algal-biofilm enhanced raceway pond for cost-effective wastewater treatment and value-added products production
Saratale et al. Microalgae cultivation strategies using cost–effective nutrient sources: Recent updates and progress towards biofuel production
Gao et al. A novel algal biofilm membrane photobioreactor for attached microalgae growth and nutrients removal from secondary effluent
Zhang et al. Promising solutions to solve the bottlenecks in the large-scale cultivation of microalgae for biomass/bioenergy production
CN105712490B (en) A kind of method of mixotroph nutrition conversion processing high ammonia-nitrogen wastewater
Zhou et al. Microalgae cultivation and photobioreactor design
JPWO2017217116A1 (en) New microalgae with aggregation ability
CN103103130B (en) Production method for lipid through mixed culture of microbes
CN102465098B (en) Culture medium composition for culturing chlorella
CN103003413A (en) Process for production of euglena containing wax ester at high content, and process for production of wax ester
CN105648023B (en) A kind of method that microalgae mixed culture prepares grease
CN102311921B (en) Method for culturing chlorella
CN109626584A (en) A kind of method of microalgae processing sauce waste water
CN102311924A (en) Method for open-type culture of microalgae
CN103103129A (en) Production method for lipid through synchronous mixed culture of microbes
CN109456905B (en) Cryptococcus rhodochrous for promoting microalgae to utilize sucrose and application thereof
CN105713934B (en) A method of producing microalgae grease
Hariz et al. Growth and biomass production of native microalgae Chlorella sp., chlamydomonas sp. and Scenedesmus sp. cultivated in Palm Oil Mill Effluent (POME) at different Cultivation conditions
Zhao et al. Microalgae cultivation
CN105836890B (en) Application of the small chlorella HB-1 of autoflocculation in the water outlet of purifying pig-raising anerobic sowage
CN105713935B (en) A kind of method of microalgae mixed culture production grease
CN105713951B (en) Method for preparing microalgae grease
CN107699493B (en) Microalgae cultivation method
CN103086582A (en) Methane preparation method

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant