CN105836890B - Application of the small chlorella HB-1 of autoflocculation in the water outlet of purifying pig-raising anerobic sowage - Google Patents
Application of the small chlorella HB-1 of autoflocculation in the water outlet of purifying pig-raising anerobic sowage Download PDFInfo
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- CN105836890B CN105836890B CN201610213039.0A CN201610213039A CN105836890B CN 105836890 B CN105836890 B CN 105836890B CN 201610213039 A CN201610213039 A CN 201610213039A CN 105836890 B CN105836890 B CN 105836890B
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
- C02F3/322—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae use of algae
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- Environmental & Geological Engineering (AREA)
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The present invention provides a kind of application of small chlorella (Chlorella minutissima) HB-1 in purified treatment pig raising anerobic sowage water outlet.Small chlorella (Chlorella minutissima) HB-1 of the invention has autoflocculation characteristic, has good catharsis to pig raising anerobic sowage water outlet, can effectively remove ammonia nitrogen, TN, TP and COD.Therefore, small chlorella of the invention (Chlorella minutissima) HB-1 can be applied in the purified treatment of pig raising anerobic sowage water outlet.
Description
Technical field:
The invention belongs to sewage treatment fields, and in particular to the small chlorella HB-1 of autoflocculation is in purifying pig-raising anerobic sowage
Application in water outlet.
Background technique:
As China's pig breeding industry is intensive and the fast development of scale, the excrement and urine waste of large-scale pig farm discharge is also in play
Increase.Excrement and urine waste is handled through anaerobic fermentation, and most of organic matter is removed, but the indexs such as N, P and COD are still very high, if greatly
The high concentration anaerobic effluent direct emission without subsequent processing is measured, considerable damage necessarily is caused to environment.Currently, anaerobic effluent
Method for subsequent processing mainly has sequencing batch active sludge or intermittent activated sludge process (SBR) and biological contact oxidation process etc.
Technique, but its complex process, energy consumption and higher cost.Thus, the other deep treatment methods for seeking anaerobic effluent seem especially
It is important and urgent.
Microalgae (Microalgae) has the characteristics that growth rate is fast, photosynthetic efficiency is high, is easy to cultivate, growth cycle is short,
The elements such as N, P in sewage can be absorbed during growth, and clean-up effect is played to water body.In addition, microalgae frond is rich in
The nutriments such as protein, vitamin and amino acid can be used as high-quality bait and feed addictive, have economic value.Pig raising
Rich in nutrients such as C, N, K, P, Cu, Fe, Zn and Ca in anerobic sowage water outlet, supply nutrients for the growth of microalgae.Using micro-
Algae purifies pig raising anerobic sowage water outlet, it can be achieved that sewage harmless treatment, water resource recycle and microalgae biology turns
The multiple effect of change has the characteristics that easy to operate, low energy consumption, less investment and economic benefit height etc., easily promotes and applies.Due to micro-
Frustule is generally less than 30 μm, and negatively charged, for density close to water, these characteristics make microalgae cell in water often in steady
Fixed suspended state is difficult to realize natural separation by gravity precipitating as activated sludge.Currently, microalgae recovery method master
There are centrifugation, filtering, flocculation, air bearing etc., the 20%-30% that harvesting link accounts for entire micro algae biomass cost is even higher, is
Limit the bottleneck of its industrial applications.
Small chlorella (Chlorella minutissima) is one kind of Chlorella (Chlorella).Chlorella
(Chlorella) it is an important category in green alga chlorella section, including small chlorella (Chlorella
Minutissima), chlorella ellipsoidea (Chlorella ellipsoidea), chlorella (Chlorella vulgaris) and egg
About 10 kinds of white nucleus chlorella (Chlorella pyrenoidosa) etc..Biological combustion is carried out by raw material of small chlorella at present
The research report of material production is more, but has no its research report gone out in Water warfare in pig raising anerobic sowage.
Summary of the invention:
The object of the present invention is to provide the small chlorella of autoflocculation (Chlorella minutissima) HB-1 at purification
Application in reason pig raising anerobic sowage water outlet.
Small chlorella (Chlorella minutissima) HB-1 of the invention has autoflocculation characteristic, dirty to pig raising
Water anaerobic effluent has good catharsis, can effectively remove ammonia nitrogen, TN, TP and COD.Therefore, of the invention small small
Ball algae (Chlorella minutissima) HB-1 can go out in purifying water process to apply in pig raising anerobic sowage.
Small chlorella (Chlorella minutissima) HB-1 of the invention was preserved in good fortune on 03 25th, 2015
Build the collection of institute of microbiology, province, deposit number are as follows: FIM-201503250001.The bacterial strain of the collection is external
Sale, therefore those skilled in the art can buy the bacterial strain from the collection from after on 03 25th, 2015.
Small chlorella (Chlorella minutissima) HB-1 of the invention also disclosed on May 19th, 2015 non-
(webpage), network address in patent document are as follows: http://www.fjim.org.cn/kytd/ktz/getNews.jsp? newsid
=220, algae applicant also holds, and guarantees to provide in 20 years to the public from the applying date.
Specific embodiment:
The following examples are further illustrations of the invention, rather than limiting the invention.
The culture of the small chlorella of embodiment 1 (Chlorella minutissima) HB-1 and the analysis of autoflocculation characteristic
Picking small chlorella HB-1 algae on a small quantity, is added to and fills 200mL grown cultures from vacuum freeze drying pipe
In the triangular flask of base, illuminance 5000lx, light dark period be 12h it is bright/12h is dark, 26 DEG C of cultures are shaken every day triangular flask 2~3
It is secondary.Culture solution color gradually greening can be observed after 3~5d.It is to be grown to obtain seed liquor (algae solution) to logarithmic growth phase, it measures
80mL algae solution, concussion 30s are uniformly mixed, and draw 5mL, measure the OD (t of algae solution at this time at 680nm immediately0) value.Remaining algae solution
60min is stood, the algae solution of same liquid level is taken, measures OD (t at this time1) value.If three parallel control groups.As follows
Calculate frond autoflocculation (from settling) activity.
The result shows that being stored at room temperature 60min, the flocculating rate of small chlorella HB-1 is up to 80% or more.
Growth media components: glucose 3.0g, yeast powder 0.2g, peptone 1.5g, sodium acetate 1.6g, sodium chloride
0.6g, dipotassium hydrogen phosphate 0.5g, bitter salt 1.5g, ammonium chloride 1.9g, liquid microelement 1mL, surplus are water, are settled to
1L, NaOH or HCL adjust pH to 6.0~7.5.It is sub-packed in 500mL triangular flask.121 DEG C of high pressure sterilization 15min.Microelement
Mother liquor: MnCl2·4H2O 12.98g, CuSO4·5H2O 1.83g, ZnSO4·7H2O 3.20g, constant volume to 1L.
Domestication tests of the small chlorella HB-1 of 2 autoflocculation of embodiment to pig raising anerobic sowage water outlet
The domestication culture of wastewater from pig farm anaerobic effluent: the small chlorella of Example 1 is carried out to small chlorella HB-1
The seed liquor 20mL, 8000r/min of HB-1 is centrifuged 5min, abandons supernatant and obtains frond, frond is inoculated into 200mL domestication culture medium
1 (volume ratio, 10% pig raising anerobic sowage water outlet+80%H2O+10% growth medium (with embodiment 1)) in, illuminance
5000lx, light dark period be 12h it is bright/12h is dark, 26 DEG C of cultures are shaken every day triangular flask 2~3 times, culture solution color can be observed
Gradually greening grows to logarithmic phase to it and inoculates domestication culture medium 2 (volume ratio, 20% pig raising anerobic sowage water outlet+70%
H2O+10% growth medium) in domestication.Step up the ratio of pig raising anerobic sowage water outlet in domestication culture medium, and synchronous mirror
The presence of inspection observation frond cell.After domestication, small chlorella HB-1 can be using tolerable concentration supporting as volume fraction 40%
The water outlet of pig anerobic sowage.Thus the autoflocculation for obtaining the pig raising anerobic sowage water outlet for being resistant to high concentration by domestication is small small
Ball algae HB-1.
Cleaning test of the small chlorella HB-1 of 3 autoflocculation of embodiment to pig raising anerobic sowage water outlet
The small chlorella HB-1 algae solution of the autoflocculation of the enduring high-concentration anaerobic effluent by domestication is taken, by 1~2% body
Product percentage is added in the triangular flask for filling 200mL growth medium (with embodiment 1), illuminance 5000lx, light dark period
For 12h it is bright/12h is dark, 26 DEG C of cultures are shaken every day triangular flask 2~3 times, culture to logarithmic growth phase.50mL algae solution is taken,
8000r/min is centrifuged 5min, abandons supernatant and obtains frond.Frond accesses 1L treatment fluid and (prepares 40% pig raising by percent by volume
Anerobic sowage water outlet+60%H2O, at the same using the mass percent of 0.1~0.3g/L add glucose) in as experimental group, together
When using do not add frond as blank control group, set up three parallel laboratory tests.Illuminance 5000lx, light dark period 12h
Bright/12h is dark, 26 DEG C of cultures, using intermittent aeration mode, i.e. aeration 2h, stops 1h.After 4d be measured by sampling water body in ammonia nitrogen, TN,
The content of TP and COD.
As a result, it has been found that it is inoculated with the experimental group of the small chlorella HB-1 of autoflocculation, the ammonia in pig raising anerobic sowage water outlet
It is 70%, TP average removal rate be 69%, the COD degradation rate that is averaged is 67% that nitrogen average removal rate, which is 83%, TN average removal rate,.
Frond stands 60min naturally, and flocculating rate is up to 80% or more.Frond crude protein content is 60% or so.Frond biomass is (dry
Weight) up to more than 1.8~2.0g (Ld).And it is not inoculated with the blank control group of the small chlorella HB-1 of autoflocculation, ammonia nitrogen,
The content of TN, TP and COD do not have significant change.
Above-mentioned test result shows that small chlorella HB-1 has good clean-up effect to pig raising anerobic sowage water outlet,
Using the characteristic of the autoflocculation of HB-1, the harvesting of frustule can be fast and effeciently carried out.It can be used as biology after frustule harvesting
Bait or feed addictive realize that pig raising anerobic sowage water outlet harmless treatment, water resource recycle and microalgae bioconversion
Multiple effect.
Claims (1)
1. small chlorella (Chlorella minutissima) HB-1 answering in purified treatment pig raising anerobic sowage water outlet
With the deposit number of the small chlorella (Chlorella minutissima) HB-1 are as follows: FIM-201503250001.
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