CN105087412B - Cultivate the method for photosynthetic microorganism and the method for production grease - Google Patents
Cultivate the method for photosynthetic microorganism and the method for production grease Download PDFInfo
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- CN105087412B CN105087412B CN201410205803.0A CN201410205803A CN105087412B CN 105087412 B CN105087412 B CN 105087412B CN 201410205803 A CN201410205803 A CN 201410205803A CN 105087412 B CN105087412 B CN 105087412B
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Abstract
The invention discloses a kind of method for cultivating photosynthetic microorganism, methods described is included under conditions of culture photosynthetic microorganism, the culture of photosynthetic microorganism is carried out in the device of culture photosynthetic microorganism, the condition of the culture photosynthetic microorganism includes driving photosynthetic microorganism motion when intensity of illumination shines intensity I more than Critical Light, stop driving when intensity of illumination shines intensity I less than or equal to Critical Light, wherein, I=A × I0, A=0.1 0.6, I0For the maximum photosynthetic efficiency intensity of illumination of the photosynthetic microorganism.A kind of method for producing grease is also disclosed, methods described includes the separation and extraction of both culturing microalgae, microalgae recovery, microalgae grease, and both culturing microalgae is cultivated using the method for culture photosynthetic microorganism as described above.The inventive method can not only ensure the normal growth of photosynthetic microorganism but also reduce energy consumption, the large-scale production suitable for photosynthetic microorganism.
Description
Technical field
The present invention relates to the method for culture photosynthetic microorganism and the method for production grease.
Background technology
Photosynthetic microorganism mainly includes microalgae and photosynthetic bacteria, many useful due to that can be extracted from photosynthetic microorganism
Product, such as grease, protein and pigment etc., therefore it is extensive, high efficiency, low cost are trained as Jiao of concern
Point.
Microalgae is that one kind grows in water, species is various and the extremely extensive rudimentary plant of distribution, and it is driven by sunlight
Dynamic cell factory.Microalgae cell absorbs CO by efficient photosynthesis2, chemical energy is converted light energy into, is stored in fat
Or in the carbohydrate such as starch, and release O2.It can reach " replacementization simultaneously with chemicals using microalgae production bioenergy
The stone energy, reduce CO2The purpose of discharge, purification waste gas and sewage ".The advantage of " Microalgae biotechnology " is following side
Face：1. microalgae is photosynthetic efficiency highest rudimentary plant, compared with crops, the yield of unit area is higher by decades of times.Microalgae
And a kind of plant the most rapid is grown in nature, generally in 24h, biomass contained by microalgae " can be referred to double at it
Its biomass doubling time can shorten to 3.5h in number growth period ".2. microalgae can be grown in high salt, the water body of high alkali environment
In, beach, salt-soda soil, desert can be made full use of to carry out large-scale culture, it is non-that seawater, saline-alkali water, industrial wastewater etc. can also be used
Agricultural water is cultivated, and therefore, microalgae can be striven ground with different crops, strive water.3. oil productivity is high, the oil content of microalgae stem cell
Up to 70%, microalgae does not have the cell differentiations such as the root, stem and leaf of higher plant, and under the conditions of nitrogen stress etc., some unicellular microalgaes can
A large amount of accumulation greases, are most promising oil-producing organisms.4. the culture of microalgae can utilize the CO in industrial waste gas2, alleviate temperature
The discharge of room gas, the NO in industrial waste gas can also be absorbedx, reduce the pollution of environment.5. produce the same of microalgae biodiesel
When, a considerable amount of microalgae biomass can also be produced, can also further obtain the high value pair such as protein, polysaccharide, aliphatic acid
Product.
Microalgae can be divided into protokaryon algae and Eukaryotic Algae, and protokaryon algae containing chlorophyll a, does not form cell based on blue-green algae
Device, photosynthesis can be carried out, protein content is high in cell, and up to the 70% of dry weight, fat content is low, is 5% or so；Eucaryon
Algal kind is relatively more, is the source of main bio-fuel algae kind.Common microalgae is predominantly due to following eight classes：Diatom
Door (Bacillariophyta), Chlorophyta (Chlorophyta), Chrysophyta (Chrysophyta), Cyanophyta
(Cyanophyta), Pyrrhophyta (Pyrroptata), Euglenophyta (Rhodophyta), Cryptophyta (Cryptophyta) and xanthophyta
Door (Xanthophyta).Wherein, Bacillariophyta, Chlorophyta and Chrysophyta are most potential biodiesel algae kind sources.
Photosynthetic bacteria is that occur generally existing in earliest, nature on the earth, have the protokaryon of original luminous energy synthetic system
Biology, it is the general name for not put the photosynthetic bacterium of oxygen under anaerobic, is a kind of leather for not forming gemma ability
Lan Shi negative bacteriums, be it is a kind of using light as the energy, can be under anaerobism illumination or aerobic dark condition using organic in nature
Thing, sulfide, ammonia etc. carry out photosynthetic microorganism as hydrogen donor and carbon source.Photosynthetic bacteria is distributed widely in nature
Soil, paddy field, marsh, lake, Jiang Hai etc., it is distributed mainly on the anoxic zone that light can be transmitted in aquatic environment.It is photosynthetic thin
The suitable water temperature of bacterium is 15 DEG C -40 DEG C, and optimum water temperature is 28 DEG C -36 DEG C.In aquaculture, the nitrous in the water body that can degrade
The noxious materials such as hydrochlorate, sulfide, realization serve as bait, purify water, prevention disease, as functions such as feed addictives.Light
It is strong to close bacterial adaptation, the organic wastewater of high concentration can be restrained oneself, have to poisonous substances such as phenol, cyanogen it is certain endure and capacity of decomposition, tool
There is stronger decomposition and inversion ability.Its many characteristics, make it that there is huge application value in Non-environmental Pollution Aquiculture.
Passing just influences the principal element of photosynthetic microorganism pilot scale culture, directly affects the photosynthetic effect of photosynthetic microorganism
Rate, growth conditions and the efficiency of light energy utilization.In order that photosynthetic microorganism preferably light grows, driving culture liquid movement is very must
Want.For example, closed photo bioreactor is typically using compressed air or pump driving culture liquid movement, raceway pond reactor
Typically using paddle wheel driving culture liquid movement.But driving culture liquid movement inevitably results in the increase of culture energy consumption, therefore,
Because energy consumption caused by driving is very huge during breeding scale, turn into the major reason under aquaculture cost is in not.
In summary, exploitation both can guarantee that photosynthetic microorganism normal growth and can reduced energy consumption, while suitable for extensive raw
The method of the culture photosynthetic microorganism of production is extremely urgent.
The content of the invention
The defects of the invention aims to overcome existing cultivation photosynthetic microorganism cost high, there is provided one kind both can guarantee that
Photosynthetic microorganism normal growth and can reduces energy consumption, the method suitable for the culture photosynthetic microorganism of large-scale production.
The present inventor has found under study for action, when cultivating photosynthetic microorganism, when intensity of illumination is more than Critical Light according to strong
Photosynthetic microorganism motion is driven when spending I, stops driving when intensity of illumination shines intensity I less than or equal to Critical Light, can both ensure
Photosynthetic microorganism normal growth reduces energy consumption again, suitable for the large-scale production of photosynthetic microorganism, wherein, I=A × I0, A=0.1-
0.6, I0For the maximum photosynthetic efficiency intensity of illumination of the photosynthetic microorganism.
Therefore, to achieve these goals, on the one hand, the present invention provides a kind of method for cultivating photosynthetic microorganism, described
Method is included under conditions of culture photosynthetic microorganism, and the training of photosynthetic microorganism is carried out in the device of culture photosynthetic microorganism
Support, the condition of the culture photosynthetic microorganism includes driving photosynthetic microorganism fortune when intensity of illumination shines intensity I more than Critical Light
It is dynamic, stop driving when intensity of illumination shines intensity I less than or equal to Critical Light, wherein,
I=A × I0, A=0.1-0.6,
I0For the maximum photosynthetic efficiency intensity of illumination of the photosynthetic microorganism.
Preferably, A=0.2-0.5.
On the other hand, the invention provides a kind of method for producing grease, methods described includes both culturing microalgae, microalgae is adopted
Receive, the separation and extraction of microalgae grease, both culturing microalgae is cultivated using the method for culture photosynthetic microorganism as described above.
The inventive method can not only ensure the normal growth of photosynthetic microorganism but also reduce energy consumption, suitable for the big of photosynthetic microorganism
Large-scale production.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Brief description of the drawings
Fig. 1 is the top view for the device that photosynthetic microorganism is cultivated in the embodiment of the present invention.
Fig. 2 is the sectional side view for the device that photosynthetic microorganism is cultivated in the embodiment of the present invention.
Fig. 3 is the growth curve of the microalgae of the embodiment of the present invention 1, comparative example 1 and comparative example 2.
Fig. 4 is the growth curve of the microalgae of the embodiment of the present invention 2 and embodiment 3.
Description of reference numerals
1 cultivation region；2 temperature adjustment areas；3 interlayers；4 transparent housings；5 driving paddle wheels；6 nutrient solution entrances；7 nutrient solutions export；8 gas
Body entrance；9 gas vents；10 heat transferring medium entrances；11 heat transferring mediums export；12 dividing plates.
Embodiment
The embodiment of the present invention is described in detail below.It is it should be appreciated that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to limit the invention.
On the one hand, the invention provides a kind of method for cultivating photosynthetic microorganism, this method is included in the photosynthetic micro- life of culture
Under conditions of thing, the culture of photosynthetic microorganism is carried out in the device of culture photosynthetic microorganism, cultivates the condition of photosynthetic microorganism
Including driving photosynthetic microorganism motion when intensity of illumination shines intensity I more than Critical Light, when intensity of illumination is less than or equal to Critical Light
Stop driving during according to intensity I, wherein,
I=A × I0, A=0.1-0.6,
I0For the maximum photosynthetic efficiency intensity of illumination of photosynthetic microorganism.
In the present invention, maximum photosynthetic efficiency intensity of illumination refers to such a intensity of illumination, i.e., in certain range of light intensity
Interior, the photosynthetic efficiency of photosynthetic microorganism increases with the rising of intensity of illumination, photosynthetic when intensity of illumination rises to a certain numerical value
Illumination intensity value when efficiency reaches maximum and do not continue to improve.
It should be appreciated that other operating parameters and condition in addition to intensity of illumination, can also influence the photosynthetic of photosynthetic microorganism
Efficiency.In the present invention, in the described maximum photosynthetic efficiency intensity of illumination of measure, other operating parameters and condition are exactly normal
Operating parameter and condition during culture photosynthetic microorganism, include the motion mode and operating parameter of driving microalgae.
In the present invention, maximum photosynthetic efficiency intensity of illumination I0Chlorophyll fluorescence method or hydrogen photoproduction method can be passed through
Measure, this two methods is known to those skilled in the art, will not be repeated here.
According to the present invention, although the condition of culture photosynthetic microorganism is included when intensity of illumination shines intensity I more than Critical Light
Photosynthetic microorganism motion is driven, stops driving when intensity of illumination shines intensity I less than or equal to Critical Light, wherein, I=A × I0, A
=0.1-0.6, I0For the maximum photosynthetic efficiency intensity of illumination of photosynthetic microorganism, you can realize the purpose of the present invention, that is, ensureing
Energy consumption is reduced in the case of photosynthetic microorganism normal growth, the large-scale production suitable for photosynthetic microorganism.But under preferable case, A
=0.2-0.5, calculates gained I, photosynthetic microorganism motion is driven when intensity of illumination shines intensity I more than Critical Light, when illumination is strong
Degree stops driving when shining intensity I less than or equal to Critical Light, can better ensure that photosynthetic microorganism while energy consumption is reduced
Growth.It is therefore preferred that A=0.2-0.5.
In the present invention, the device for cultivating photosynthetic microorganism can be used commonly used in the art various without particular/special requirement
Device.For example, culture microalgae is carried out typically in bioreactor, it is anti-that bioreactor can be divided into open photo-biological
Answer device and closed photo bioreactor, Race-way photobioreactor, such as raceway pond, closed photo bioreactor, example
Such as tubular type, board-like, pillar.
As those skilled in the known, passing just influences the principal element of photosynthetic microorganism pilot scale culture, directly
Influence photosynthetic efficiency, growth conditions and the efficiency of light energy utilization of photosynthetic microorganism.During photosynthetic microorganism pilot scale culture,
It can utilize the characteristics of microalgae photosynthesis and improve photosynthetic efficiency, for example, for microalgae, when frustule is in light area and dark space
Between displacement (be usually above 1Hz) when reaching certain frequency, " flash effect " (Janssen M, Slenders will occur
P,Tramper J,Mur L R,Wijffels R.Enzyme Microbial Technology,2001,29:298-305),
The photosynthetic efficiency of microalgae can be improved.Therefore, in order to improve displacement of the photosynthetic microorganism between light area and dark space, improve photosynthetic micro-
The photosynthetic efficiency of biology, under preferable case, cultivate to be provided with the device of photosynthetic microorganism and strengthen disturbing for photosynthetic microorganism disturbance
Stream unit.
For turbulator member without particular/special requirement, as long as can strengthen photosynthetic microorganism disturbance, increase photosynthetic microorganism is in Guang Qu
Displacement between dark space, can use the thinkable various turbulator members of those skilled in the art institute, such as can be
The inwall (bottom wall, side wall) for cultivating the device of photosynthetic microorganism sets spoiler etc..
In the present invention, in order that photosynthetic microorganism receives more sufficient illumination, in the device for cultivating photosynthetic microorganism, contain
Thick preferred≤the 20cm, more preferably 3-10cm of the liquid of the nutrient solution of photosynthetic microorganism.
Photosynthetic microorganism of the present invention is selected from microalgae and photosynthetic bacteria.Specifically include green alga, blue-green algae, diatom and chrysophyceae
Etc. microalgae or the green bacterium of photosynthetic original, purple bacteria etc. can be carried out.One common feature of these photosynthetic microorganisms is
Photosynthesis can be carried out.
The inventive method can be used for low energy consumption, efficient culture photosynthetic microorganism, especially suitable for the culture of microalgae, especially
It is the microalgae of Bacillariophyta, Chlorophyta or Chrysophyta, such as chlorella, grid algae, single needle algae etc..
In the present invention, illumination can be artificial light source or natural daylight, in order to further save energy consumption, can use certainly
Right daylight.
As those skilled in the known, required for maintenance microalgae normal growth is also needed to during cultivating microalgae
Other necessary conditions, nutrition necessary to suitable illumination, temperature, and micro algae growth is such as provided, is regulated and controled in algae solution
CO2, dissolved oxygen, water, necessary nutrient matter, pH value etc. in suitable scope, make the fast-growth of its suitable microalgae with it is numerous
Grow.These technologies are well-known to those skilled in the art.For example, the condition of culture photosynthetic microorganism can include：Temperature is
15-40 DEG C, preferably 25-35 DEG C；Light intensity is 2000-200000lux, preferably 5000-100000lux；In incubation
It is passed through CO2, make algae solution pH value in the range of 6-10.
In the present invention, microalgae culture can use classification expand by the way of, such as algae kind pass through 1L, 5L, 50L,
500L ... amplifies culture step by step.The initial concentration of inoculation can be typically controlled in algae solution OD value (OD680Value) for 0.2-1's
In the range of.Culturing time is generally more than 10 days, preferably more than 15 days, and for oil-producing microalgae, culturing time more than 20 days can be with
Obtain more preferably effect.
On the other hand, present invention also offers a kind of method for producing grease, this method includes both culturing microalgae, microalgae is adopted
Receive, the separation and extraction of microalgae grease, both culturing microalgae is cultivated using the method for culture photosynthetic microorganism as described above.
Embodiment
The present invention is further illustrated for following embodiment, but and is not so limited the present invention.
In the following Examples and Comparative Examples：
Maximum photosynthetic efficiency intensity of illumination I0Assay method：Chlorophyll fluorescence method, i.e., determined with chlorophyll fluorescence instrument micro-
The quick photoresponse curve of algae, i.e. Relative electron transport rate with intensity of illumination change curve, so that it is determined that maximum photosynthetic effect
Rate intensity of illumination I0。
Algae solution OD value (OD680Value) measure：Using spectrophotometric determination, compared with distilled water, measure algae solution exists
Light absorption value at wavelength 680nm, the index as microalgae concentration.
The culture medium of microalgae：Media Components are shown in Table 1-2.
The culture medium BG11 of table 1
| Component | Content, mg/L |
| K2HPO4 | 40 |
| Na2CO3 | 20 |
| MgSO4·7H2O | 75 |
| CaCl2·2H2O | 36 |
| Citric acid | 6 |
| Ferric citrate | 6 |
| EDETATE SODIUM | 1 |
| Micro- A5 | 1 |
2 micro- A5 of table
| Component | Content, mg/L |
| H3BO3 | 2860 |
| MgCl2·4H2O | 1810 |
| ZnSO4·7H2O | 22 |
| CuSO4·5H2O | 7.9 |
Using the device of the culture photosynthetic microorganism shown in Fig. 1 and Fig. 2：Cultivation region 1 and temperature adjustment area 2 are cuboid, training
The top surface for supporting the bottom surface and temperature adjustment area 2 in area 1 is in close contact by interlayer 3, and on the contact surface, cultivation region 1 and temperature adjustment area 2 are isometric,
The width in temperature adjustment area 2 is more than the width of cultivation region 1.Cultivation region 1 be provided with transparent housing 4, driving paddle wheel 5, nutrient solution entrance 6,
Nutrient solution outlet 7, gas access 8, gas vent 9, and the widthwise central in cultivation region is provided with dividing plate 12 along its length, makes
Nutrient solution containing chlorella circulates under the driving of driving paddle wheel 5 around dividing plate 12.Temperature adjustment area 2 is provided with heat transferring medium
Entrance 10 and heat transferring medium outlet 11, heat transferring medium is water, full of temperature adjustment area 2.When starting driving paddle wheel 5, make containing microalgae
Flow velocity of the nutrient solution in cultivation region 1 is 1m/s.
Embodiment 1
The present embodiment is used for the method for the culture photosynthetic microorganism for illustrating the present invention.
Chlorella (being purchased from aquatile research institute of the Chinese Academy of Sciences) is cultivated in the device of culture photosynthetic microorganism.Determine bead
The I of algae0=30000lux, coefficient A=0.2 is taken, calculate I=6000lux.Using BG11 culture mediums, nitrogenous fertilizer is 0.3g/L urea,
Nutrient solution liquid thickness is 5cm, and it is 25-35 DEG C to control culture-liquid temp by temperature adjustment area 2, algae kind initial concentration OD680For 0.5, it is passed through
2% (v/v) CO2Ventilation culture, intake make it that algae solution pH value is in the range of 6-10 in incubation.Adopted in incubation
Manually light source and daylight mixed culture, it is 40000lux to control intensity of illumination daytime (12h), night (12h) artificial light source light
It is 6000lux according to intensity.Start driving paddle wheel 5 daytime and drive algae solution shuttling movement, night stops paddle wheel driving.Detection algae daily
The OD of liquid680Value, continuous culture harvest after 20 days, and its growth curve is shown in Fig. 3.
Embodiment 2
The present embodiment is used for the method for the culture photosynthetic microorganism for illustrating the present invention.
Grid algae (being purchased from aquatile research institute of the Chinese Academy of Sciences) is cultivated in the device of culture photosynthetic microorganism.Determine grid algae
I0=30000lux, coefficient A=0.5 is taken, calculate I=15000lux.Using BG11 culture mediums, nitrogenous fertilizer is 0.3g/L urea, training
Nutrient solution liquid thickness is 8cm, and it is 25-35 DEG C to control culture-liquid temp by temperature adjustment area 2, algae kind initial concentration OD680For 0.5, it is passed through
2% (v/v) CO2Ventilation culture, intake make it that algae solution pH value is in the range of 6-10 in incubation.Adopted in incubation
Manually light source and daylight mixed culture, it is 40000lux to control intensity of illumination daytime (12h), night (12h) artificial light source light
It is 15000lux according to intensity.Start driving paddle wheel 5 daytime and drive algae solution shuttling movement, night stops paddle wheel driving.Detection daily
The OD of algae solution680Value, continuous culture harvest after 12 days, and its growth curve is shown in Fig. 4.
Embodiment 3
The present embodiment is used for the method for the culture photosynthetic microorganism for illustrating the present invention.
Single needle algae (being purchased from aquatile research institute of the Chinese Academy of Sciences) is cultivated in the device of culture photosynthetic microorganism.Determine single needle
The I of algae0=30000lux, coefficient A=0.3 is taken, calculate I=9000lux.Using BG11 culture mediums, nitrogenous fertilizer is 0.3g/L urea,
Nutrient solution liquid thickness is 10cm, and it is 25-35 DEG C to control culture-liquid temp by temperature adjustment area 2, algae kind initial concentration OD680For 0.5, lead to
Enter 2% (v/v) CO2Ventilation culture, intake make it that algae solution pH value is in the range of 6-10 in incubation.In incubation
It is mixed using artificial light source and daylight, it is 40000lux to control intensity of illumination daytime (12h), night (12h) artificial light source
Intensity of illumination is 9000lux.Start driving paddle wheel 5 daytime and drive algae solution shuttling movement, night stops paddle wheel driving.Detection daily
The OD of algae solution680Value, continuous culture harvest after 12 days, and its growth curve is shown in Fig. 4.
Comparative example 1
According to the method culture photosynthetic microorganism of embodiment 1, the difference is that, control driving paddle wheel 5 whole day 24h is rotated, often
The OD of its detection algae solution680Value, continuous culture harvest after 20 days, and its growth curve is shown in Fig. 3.
Comparative example 2
According to the method culture photosynthetic microorganism of embodiment 1, the difference is that, take coefficient A=0.7, control on daytime (12h) light
It is 40000lux according to intensity, night (12h) artificial light source intensity of illumination is 21000lux.Start driving paddle wheel 5 daytime and drive algae
Liquid shuttling movement, night stop paddle wheel driving.The OD of detection algae solution daily680Value, continuous culture harvest after 20 days, and it grows bent
Line is shown in Fig. 3.
From figs. 3 and 4 it can be seen that the method for the culture photosynthetic microorganism of the present invention can ensure photosynthetic microorganism just
It is frequently grown, because the inventive method drives photosynthetic microorganism to move only when intensity of illumination shines intensity I more than Critical Light, therefore pole
The earth reduces energy consumption.
In Fig. 3, by embodiment 1 as can be seen that the inventive method can ensure photosynthetic microorganism compared with comparative example 1
Normal growth, and due to driving photosynthetic microorganism motion only when intensity of illumination shines intensity I more than Critical Light, therefore greatly
Reduce energy consumption；Embodiment 1 can be ensured photosynthetic micro- compared with comparative example 2 as can be seen that as A=0.1-0.6
The normal growth of biology.
The inventive method can not only ensure the normal growth of photosynthetic microorganism but also reduce energy consumption, suitable for the big of photosynthetic microorganism
Large-scale production.
The preferred embodiment of the present invention is described in detail above in association with accompanying drawing, still, the present invention is not limited to above-mentioned reality
The detail in mode is applied, in the range of the technology design of the present invention, a variety of letters can be carried out to technical scheme
Monotropic type, these simple variants belong to protection scope of the present invention.
It is further to note that each particular technique feature described in above-mentioned embodiment, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy no longer separately illustrates.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should equally be considered as content disclosed in this invention.
Claims (9)
1. a kind of method for cultivating photosynthetic microorganism, methods described is included under conditions of culture photosynthetic microorganism, in culture light
Close the culture that photosynthetic microorganism is carried out in the device of microorganism, it is characterised in that the condition of the culture photosynthetic microorganism includes
Photosynthetic microorganism motion is driven when intensity of illumination shines intensity I more than Critical Light, when intensity of illumination is less than or equal to Critical Light according to strong
Stop driving when spending I, wherein,
I=A × I0, A=0.2-0.5,
I0For the maximum photosynthetic efficiency intensity of illumination of the photosynthetic microorganism.
2. according to the method for claim 1, wherein, it is photosynthetic micro- to be provided with reinforcing in the device of the culture photosynthetic microorganism
Bioturbated turbulator member.
3. the method according to claim 11, wherein, in the device of the culture photosynthetic microorganism, containing described photosynthetic
Liquid thickness≤20cm of the nutrient solution of microorganism.
4. according to the method for claim 3, wherein, the liquid thickness of the nutrient solution is 3-10cm.
5. according to the method for claim 1, wherein, the photosynthetic microorganism is selected from microalgae and photosynthetic bacteria.
6. according to the method for claim 5, wherein, microalgae is selected from the microalgae of Bacillariophyta, Chlorophyta and Chrysophyta.
7. according to the method for claim 6, wherein, microalgae is selected from least one of chlorella, grid algae and single needle algae.
8. according to the method for claim 7, wherein, the condition of the culture photosynthetic microorganism includes：Temperature is 15-40
DEG C, light intensity 2000-200000lux.
9. a kind of method for producing grease, methods described includes the separation and extraction of both culturing microalgae, microalgae recovery, microalgae grease,
Characterized in that, both culturing microalgae uses the method for the culture photosynthetic microorganism described in any one in claim 1-8 to be supported
Grow.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1201826A (en) * | 1997-06-05 | 1998-12-16 | 华南师范大学 | Spirulina light illuminating tower-tray optic bio-reactor culturing system and its controlling method |
| CN1400303A (en) * | 2002-08-25 | 2003-03-05 | 常军 | Preparation method of photosynthetic bacterium preparation |
| CN101088966A (en) * | 2006-06-13 | 2007-12-19 | 北京新纪元三色生态科技有限公司 | Microbial fertilizer and its prepn process and application |
| CN101525576A (en) * | 2009-04-14 | 2009-09-09 | 中华绿油有限公司 | Method for producing microalgae with domestic sewage and industrial sewage |
| CN101705190A (en) * | 2009-11-29 | 2010-05-12 | 中南大学 | Chlorella sorokiniana CS-01 and culture method thereof for producing grease |
| CN102311920A (en) * | 2010-07-07 | 2012-01-11 | 中国石油化工股份有限公司 | Culture method for chlorella |
-
2014
- 2014-05-15 CN CN201410205803.0A patent/CN105087412B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1201826A (en) * | 1997-06-05 | 1998-12-16 | 华南师范大学 | Spirulina light illuminating tower-tray optic bio-reactor culturing system and its controlling method |
| CN1400303A (en) * | 2002-08-25 | 2003-03-05 | 常军 | Preparation method of photosynthetic bacterium preparation |
| CN101088966A (en) * | 2006-06-13 | 2007-12-19 | 北京新纪元三色生态科技有限公司 | Microbial fertilizer and its prepn process and application |
| CN101525576A (en) * | 2009-04-14 | 2009-09-09 | 中华绿油有限公司 | Method for producing microalgae with domestic sewage and industrial sewage |
| CN101705190A (en) * | 2009-11-29 | 2010-05-12 | 中南大学 | Chlorella sorokiniana CS-01 and culture method thereof for producing grease |
| CN102311920A (en) * | 2010-07-07 | 2012-01-11 | 中国石油化工股份有限公司 | Culture method for chlorella |
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